Product Data Sheet

COROLASE® 8000
Rev. No.: 03
Valid from: 24/02/2019

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DESCRIPTION GMO STATUS

COROLASE® 8000 is a thermotolerant, food-grade, fungal, COROLASE® 8000 and its constituent enzyme are not
alkaline protease enzyme preparation ideal for hydrolyzing genetically modified.
proteins in mildly alkaline conditions. Specifically, it is a serine
The enzyme component is produced by fermentation of a
endo-protease (thermomycolin) and can also be described as
genetically modified microorganism, which is removed and
an endopeptidase.
not present in the final product.
PROPERTIES Only agricultural raw materials of non-GM origin are used for
the fermentation process and final formulation.
Declared enzyme Fungal alkaline protease
Declared activity 100,000 BPU/g STORAGE CONDITIONS AND SHELF LIFE
Production strain Trichoderma reesei
IUB number EC 3.4.21.65 Recommended storage: Store cool below 10 °C.
CAS number 52233-31-5 The best before date is displayed on the certificate of analysis
Physical form Liquid and product label.
Color Light brown
Density 1.1 The product should be used within 24 months of the
production date. Thereafter, reanalysis is recommended.
Color and appearance may vary from batch to batch. Color intensity is
not an indication of enzyme activity.
PACKAGING
APPLICATION AND BENEFITS
SAP no. Package size Material
12294 25 kg PE canister
COROLASE® 8000 can be used for many sorts of protein 12293 1,000 kg IBC container
hydrolysis, regardless of whether they are vegetal or animal in
origin. It can be used to achieve a high degree of hydrolysis. Palletized with 24 units per pallet (dimensions 120x80x110 cm).

COROLASE® 8000 can hydrolyze a broad range of proteins at The packaging is in conformity with the requirements for raw
and above neutral pH. It may be used either on its own or in materials used in the packaging of foods as defined by the
combination with other proteases or enzyme activities. Commission Directives (EU) and FDA (USA) regulations.

Typical applications include the hydrolysis of wheat gluten,

COMPLIANCE AND LEGAL STATUS
soya protein, gelatin, and milk proteins, recycling of meat off
cuts, and recovery of fish protein from by-products.
Our enzyme products are used as processing aids in the food
DOSAGE manufacturing process and are thus free from any labeling
provisions in the European Union.

The dosage of the enzyme depends on the raw materials used, The product does not fall within the scope of EU regulations
reaction conditions such as pH, temperature and time, and the (EC) 1829/2003 and (EC) 1830/2003 on genetically modified
desired degree of hydrolysis. The optimal dosage should be food and feed.
determined in application trials. The product fulfills the purity specifications of the Joint
FAO/WHO Expert Committee on Food Additives (JECFA) and
pH range: 6.0–10.0 Food Chemicals Codex (FCC) for food-grade enzymes.
Temperature range: up to 80°C
COROLASE® 8000 is approved in all EU Member States. Advice
For initial trials, typical dosages are: 0.1–2.0%, based on the on the legal status for other countries is available upon
protein content. request.

PRODUCT COMPOSITION SPECIAL DIET INFORMATION

Glycerol (q.s.), sorbitol (q.s.), protease concentrate, water, Kosher Certified
sodium benzoate (0.35%). Halal Certified
Constituent Amount in % Vegans We guarantee that no raw materials or
(Ovo)-(Lacto)- processing aids derived from animal origin
Enzyme concentrate 25–30 Vegetarians were utilized, and that all other
Glycerol 30 formulation components of the enzyme
product are not of animal origin.
Sorbitol 30
Certificates are provided upon request.
Sodium benzoate 0.35
Water Remainder

© AB Enzymes
COROLASE® 8000, Rev. No.: 03
AB Enzymes GmbH
Feldbergstr. 78
64293 Darmstadt
Germany
Tel. +49 6151 3680 – 100
Fax +49 6151 3680 – 120

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ALLERGENS CERTIFICATION

Ingredients referred to in Present Remark AB Enzymes GmbH and its production site ROAL Oy have a
Article 9, Article 21 and (as added certified quality management system according to ISO
Annex II of Regulation (EU) component) 9001:2015 and HACCP. AB Enzymes complies with the
No 1169/2011 (Yes/No)
“Guidance on Social Responsibility” ISO 26000:2010 standard.
Cereals containing gluten 1) No Wheat bran used as RM
in fermentation, but
considered consumed
and absent in the final ROAL Oy in Finland holds ISO 9001:2015 quality management,
product ISO 50001:2011 energy management, and ISO 14001:2015
Crustaceans and products No environmental management certification.
thereof
In addition, Food Safety Management System certification
Eggs and products thereof No
according to FSSC 22000 is in place and reviewed in regular
Fish and products thereof No
Peanuts and products No audits.
thereof
Soybeans and products No SAFETY AND HANDLING
thereof
Milk and products thereof No
Avoid unnecessary contact with enzyme preparations when
(including lactose)
Nuts 2) No
handling, and avoid the formation of aerosol and dust of the
Celery and products thereof No product. Repeated inhalation of enzyme aerosol or dust may
Mustard and products No cause sensitization, or allergic type reactions in sensitized
thereof individuals.
Sesame seeds and products No
thereof For further details on the safe handling of our products, please
Sulfur dioxide and sulfites at No consult our Safety Data Sheet.
concentrations of more than
10 mg/kg or 10 mg/l
expressed as SO 2
Lupine and products thereof No
Mollusks and products No
thereof
1)
i.e., wheat, rye, barley, oats, spelt, kamut
2)
i.e., almond, hazelnut, walnut, cashew, pecan, Brazil nut, pistachio, macadamia, and
Queensland nut

NUTRITIONAL VALUES

Typical calculated values per 100 g

Energy 983 kJ
Total fat <1 g
Saturated fat 0g
Total carbohydrate 63 g
Sugars 0g
Added sugars 0g
Sugar alcohol 30 g
Protein 5g
Salt 0g
Potassium 10 mg
Minerals <1 g
Vitamin D 0 mg

PURITY CRITERIA

Total viable count < 50,000 / g

Coliforms < 30 / g
E. coli absent in 25 g
Salmonella absent in 25 g
Antibiotic activity negative by test
Heavy metals (as Pb) < 30 mg / kg
Arsenic < 3 mg / kg
Lead < 5 mg / kg
Cadmium < 0.5 mg / kg
Mercury < 0.5 mg / kg

© AB Enzymes

COROLASE® is a registered trademark of AB Enzymes GmbH. Use and/or sale of these products may be covered by one or more patents as well as patent applications in EU, US and other countries.
Our technical advice on the uses of our products is given without obligation. AB Enzymes is not responsible for the application and processing of the products by the customer or any third party.
The customer is solely liable to comply with the applicable laws and regulations, and intellectual property rights of third parties.
This document contains product specifications that may be altered without prior notice.