USER’S MANUAL

SP-8001 UV-VIS Spectrophotometer

Metertech Inc.
Version 1.08
Metertech Inc. provides this publication “as is” without warranty of any kind, either express or implied,
including, but not limited to the implied warranties of merchantability or fitness for a particular purpose.

All rights are reserved, including those to reproduce this publication or parts thereof in any form without
permission in writing from Metertech Inc.

Information in this publication is subject to change without notice and does not represent a commitment on
the part of the vender.

Any errors or omissions which may have occurred in this publication despite the utmost care taken in its
production will be corrected as soon as possible, but not necessarily immediately upon detection.

Copyright© Metertech Inc. 1999

Metertech Inc. ‧ 63-2 Cheng Gong Rd. Sec. 1 ‧ Nangang, Taipei, Taiwan, R.O.C.
E-Mail: [email protected] ‧ Web Site: www.metertech-inc.com
Tel.: 886-2-2783-2854 ‧ Fax.: 886-2-2783-1764
 Metertech Inc.

Contents

Chapter 1 INTRODUCTION -------------------- 4

§1-1 Specification -------------------- 4
§1-2 Safety Notes -------------------- 5
Chapter 2 INSTALLATION -------------------- 6
§2-1 Unpacking -------------------- 6
§2-2 Installation -------------------- 7
Chapter 3 GENERAL -------------------- 8
§3-1 User Interface -------------------- 8
§3-2 General Function Description -------------------- 10
§3-3 Initialization -------------------- 12
Chapter 4 SYSTEM SETUP -------------------- 14
§4-1 System Setup Menu -------------------- 14
Chapter 5 PHOTOMETRIC -------------------- 18
§5-1 Photometric Parameter Setup -------------------- 18
§5-2 Photometric Function Structure -------------------- 19
§5-3 Basic Photometric Measurement -------------------- 19
Chapter 6 SPECTRUM -------------------- 23
§6-1 Spectrum Parameter Setup -------------------- 23
§6-2 Spectrum Function Structure -------------------- 24
§6-3 Basic Spectrum Measurement -------------------- 25
Chapter 7 TIMESCAN -------------------- 30
§7-1 TimeScan Parameter Setup -------------------- 30
§7-2 TimeScan Function Structure -------------------- 31
§7-3 Basic TimeScan Measurement -------------------- 32
Chapter 8 KINETIC -------------------- 34
§8-1 Kinetic Parameter Setup -------------------- 34
§8-2 Kinetic Function Structure -------------------- 36
§8-3 Basic Kinetic Measurement -------------------- 37
Chapter 9 QUANTITATIVE -------------------- 39
§9-1 Quantitative Parameter Setup -------------------- 39
§9-2 Quantitative Function Structure -------------------- 40
§9-3 Basic Quantitative Measurement -------------------- 41
Chapter 10 ACCESSORIES -------------------- 44
§10-1 Multi-Cell Holder -------------------- 44
§10-2 Sipper -------------------- 46
Chapter 11 MAINTENANCE -------------------- 48
§11-1 Routine Maintenance -------------------- 48
§11-2 Replacement of Lamps -------------------- 49
Appendix A Thermal Controller Operation -------------------- 51
Instruction
Appendix B Sipper Operation Instruction -------------------- 53
Appendix C Setting of USB Serial Port -------------------- 56

2
 Metertech Inc.

3
 Metertech Inc.

Chapter 1 INTRODUCTION

The SP-8001 UV-VIS Spectrophotometer is an instrument designed and manufactured by Metertech Inc. It
can meet the wide-ranging needs of prospective users. The main unit features fundamental photometric
functions that are ready expandable using various application software packages and attachments for
enhanced convenience and productivity. It is designed to be user-friendly and require as little engineering
knowledge as possible to adopt in users’ application.

In order to obtain maximum performance and trouble-free operation with the SP-8001, It is recommended
that you carefully read this manual before using the SP-8001, and thereafter keep it close at hand for future
reference.

§1-1 Specifications

Wavelength 190-1100nm
Bandwidth pass 2nm
Wavelength accuracy +/- 1nm
Monochromator split beam
Wavelength repeatability +/- 0.2nm
Photometric range -0.300-3.000Abs
Photometric accuracy +/- 0.005Abs at 1.000Abs by NIST SRM930e
Stability drifting < 0.0003Abs/hr at 500nm after 1 hour warm-up
Baseline flatness +/- 0.002Abs (at 210-1000nm)
Stray light < 0.05% at 340nm & 220nm by NIST SRM2031
Light source deuterium lamp (UV range) & halogen lamp (Vis range)
Source lamp change automatic
Detector Silicon photodiodes
Wavelength scanning speed 100-5000nm/min (user-programmable)
Path-length variable at 10-100 mm
Display backlight LCD – adjustable contrast
Interface serial & parallel
Power/fuse AC input: 100/120/220/240V 50/60Hz,
Fuse: T2A for 220/240V, T4A for 100/120V
Dimension 506(W)X430(D)X220(H)mm
Weight approx. 18kgs
Ambient temp/humidity room temperature 15-35℃, humidity < 80%

Specifications subject to change without notice.

4
 Metertech Inc.

§1-2 Safety Notes

Although this SP-8001 UV-VIS Spectrophotometer is user friendly, the instrument and its accessories
described in this manual are designed to be used by properly trained persons only. READ THIS SECTION
CAREFULLY BEFORE INSTALLING AND USING THE INSTRUMENT AND ITS ACCESSORIES.

9 Adjustment, maintenance, and repair of equipment must be carried out only by qualified persons who
are aware of possible hazards involved. Wherever indicated in this manual, certain maintenance may
be carried out by users, who must be fully understand and apply the following safety precautions.
9 For the correct and safe use of this instrument and its accessories it is essential that both operating and
servicing persons follow generally accepted safety procedures in addition to the safety precautions
specified in this manual.
9 Some of the chemicals used in spectrophotometer are corrosive, and/or flammable and samples may be
radioactive, toxic or potentially infective. Care should be taken to follow the normal laboratory
procedures for handling chemicals.
9 Only the persons who have been trained to avoid the risk of electric shock are qualified to remove the
instrument covers and accessories. The mains electricity supply to the instrument must be
disconnected at the mains supply connector and at least three minutes allowed for capacitors to
discharge.
9 The UV radiation from a deuterium lamp can be harmful to the skin and eyes. Always view the lamp
through protective glasses that will absorb the UV radiation and avoid looking directly at the deuterium
arc. Do not expose the skin to direct or reflected UV radiation.
9 Whenever it is likely that safety protection has been impaired, the instrument and/or accessories must
be made inoperative and secured against any unintended operation. The matter should then be
referred to appropriate service authority.

5
 Metertech Inc.

Chapter 2 INSTALLATION

BEFORE installation please read safety notes in previous page carefully. Failing to abide by the regulations
may cause damages to the instrument and may obtain incorrect results.

§2-1 Unpacking

The SP-8001 (Figure 2.1 and Figure 2.2) should be delivered with intact packaging that contains one carton
with items listed in the packing list. Unpack the carton and inspect carefully. If any part is damaged or
missing, contact your local dealer immediately.

lamp compartment sample compartment

Figure 2.1 Top view of the SP-8001

power switch power receptacle RS-232 port

waste bottle alarm fan printer port LCD contrast adjuster

Figure 2.2 Back panel of the SP-8001

Save the carton with packing materials in a proper place in case that the instrument may need to be
repacked or to be returned for service.

6
 Metertech Inc.

§2-2 Installation

Before the first installation, please inspect carefully whether any damages or suspicious defects you may be
aware of. Do the followings before turn on the instrument. Contact your local agent for help if
encountering any difficulties during installation and operation.

A. Always place the instrument on a stable and vibration-free table. Allow two inches behind the rear
panel of the instrument for air circulation and power cord, and protect the instrument from being
located:
♦ in direct sunlight
♦ in drought from window, air conditioner or heater
♦ near copy machine
♦ near refrigerator or oven
♦ near ultrasonic cleaner
♦ near X-ray equipment
♦ near centrifuge
B. Check and set the voltage. The SP-8001 can be performed with selective power supply at 100, 120,
220, or 240Vac 50/60Hz from a properly wired and grounded single phase power outlet. Check the
voltage shown in the frame above power inlet. If it is not the desired, use flat screw driver to open the
protective plastic cover. Push inside the voltage wheel and turn it into the desired position accordingly.
Close the protective plastic cover.
C. The room temperature of operating site should be between 15 - 30℃, and humidity less than 80%.
D. Install all desired accessories and connect necessary wires and tubes properly.
E. Plug the power cord into the instrument inlet and to an appropriate wall outlet.
F. Now the system is ready for performance. Turn power on and refer details of operation to specific
sections.

7
 Metertech Inc.

Chapter 3 GENERAL

The SP-8001 is designed to be a self-contained instrument and can work as a standalone machine or can
work with other peripherals together.

The SP-8001 has one membrane-type keyboard and a 320X240 LCD display as the user interface. User
may key in data through the keyboard and proper messages will show on the display. A lamp compartment
facilitates a tungsten halogen lamp and a deuterium lamp that users are allowed to replace those lamps
whenever needed. READ SAFETY NOTES in section §1-2 carefully and instructions in section §11-2 before
changing lamps. A sample compartment is the area for users manipulating their samples. Also it allows
users to install proper accessories for specific operations.

§3-1 User Interface

The SP-8001 keyboard with LCD display module is shown in Figure 3.1. Operations of those keys are
designed to be user friendly. In most of cases, users press keys according to instructions shown in the
corresponding screens. Otherwise, refer to specific sections of different operation modes for more details.

Figure 3.1 Keyboard with LCD display module

For operations with optional accessories, refer to proper sections in Chapter 10.

General explanation of keys is given below:

Function keys Selecting and performing supporting functions shown in the screens.
Numerical keys For entering numbers, minus, and decimal point
λ For entering desired wavelength.
CLEAR To clean up unwanted input digits in the entry field.
ESC To quit the current screen and go back to the previous screen, or to quit from the
current process, or to go back to upper level of function key screen.
QUICK RUN To pop up a Parameter File List (Figure 3.2) for users to select operation settings

8
 Metertech Inc.

stored in memory. In this file list, users can view all parameter settings in
different operation modes that is distinguished by the first 2 letters in the first
column. For example, PH represents for “Photometric” mode. Filenames are
shown in the second column, while the corresponding time in the third column.

READ To start measuring process according to the current settings.

AUTO ZERO To perform blank correction when needed. It sets the photometric value to 0ABS

12:00:00 QUICK RUN 500.0nm 0.000A

Parameter file list
--------------------------------------
No Name Time
PH-01 ART01 07/01/99 10:12:32
PH-02
PH-03
PH-04
SP-05 HOLMIUM 07/12/99 09:30:12
SP-06
SP-07
SP-08
TS-09 LIGHT01 06/24/99 13:22:43
TS-10 LIGHT02 06/25/99 15:32:33
TS-11
TS-12
KI-13 GOT01 03/21/99 16:37:21
KI-14
KI-15
KI-16
Press UP DOWN key to select...

Figure 3.2 Quick Run Parameter File List screen

(100%T) after AUTO ZERO being pressed.

ENTER To confirm selections or entries of fields or parameters.
Arrow keys To move the cursor between entry fields or parameters. Left/right arrow keys
are also used for switching Yes/No as instructed.
CELL ∧ To move sample holder one position up as needed.
CELL ∨ To move sample holder one position down as needed.

9
 Metertech Inc.

§3-2 General Function Description

Functions being used in the SP-8001 are listed in the corresponding modes and are shown in structured
maps. For those functions being widely used in most of the modes will be described below.

Load Param For loading existing parameter settings stored in memory. When this
function is pressed, a parameter file list screen (Figure 3.3) shows up
and users can choose from the list by using arrow keys and followed
by an ENTER key. Only the mode-relevant parameter setting files
will appear when function Load Param is pressed.

Save Param For saving a current settings into memory. Filename is entered

12:00:00 PHOTOMETRIC 500.0nm 0.000A

Parameter file list
--------------------------------------
No Name Time
01 ART01 07/01/99 10:12:32
02
03
04
05
06
07
08
09
10
11
12
13
14
15
Press UP DOWN key to select...

Figure 3.3 Parameter file list in photometric mode

through a 4 by 9 array-type table (Figure 3.4). By using up/down

arrow keys for choosing rows and then numerical keys for choosing
column, the desired alphabet or number will be picked up. 10
alpha-numerical keys are the maximum for a filename. Using left
arrow key to delete the input. An ENTER key will close the input
procedure and the Parameter File List screen will show up. Using
up/down arrow keys to choose a place for the filename and followed
by an ENTER key will complete the Save Param procedure. A time
tag will be automatically attached to the saved filename. Press
function Load Param and you may see what has just been done.

Print Data To print data or graph from the current screen.

Sample Control To be functioning only when certain accessory being used.
10
 Metertech Inc.

12:00:00 500.0nm 0.000A

1 2 3 4 5 6 7 8 9

A B C D E F G H I

J K L M N O P Q R

>>> S T U V W X Y Z 0

1 2 3 4 5 6 7 8 9

File Name:ART

Press UP DOWN key to select...

Figure 3.4 Filename input screen

Next Screen Accept the current settings and switch into the measuring display
screen. Once get the sample(s) ready, press READ for proceeding
the measurement according to the setup. Sometimes, it is used for
tracing back to the previous level of functions or for showing the
previous graph on the screen.
Prev Screen Leave the current display screen and switch back to a previous display
screen.
Manipulate For further manipulation of current data either from new
measurement or from existing data files.
Clear Graph To clean up the current screen. This will result in loss of data in
previous measurement. Only those data for the most current
measurement can be rebuilt with function Original under function
Manipulate.
Load Data To load data with determined parameter setup from the associated
Data File List.
Save Data To save the data of current measurement or to save the data of
chosen point in function Show Track. This will also save the settings
of parameters in that measurement. Message “No data present …”
will show if there is no data in the screen.

11
 Metertech Inc.

§3-3 Initialization

Turn on the power of the SP-8001, a start-up screen (Figure 3.5) shows up and the initialization of system
will be automatically done. The version of software coming up with the instrument is also shown in the
start-up screen. User-installed accessory will be automatically detected during the initialization.

12:00:00 0.0nm 0.000A

Metertech
Metertech Inc. SP-8001
UV/VIS SPECTROPHOTOMETER
All rights reserved.
Version 1.3

Detect Accessory...Single-Cell
Initial System.....

Figure 3.5 Start-up screen

Following the initialization, the System Main Menu screen will show up (Figure 3.6). Six items representing
six operation modes are available for users to select as required. Users may use numerical keys or arrow
keys, followed by an ENTER key, to complete the selection of desired mode.

12:00:00 500.0nm 0.000A

System Main Menu

1.Photometric
2.Spectrum
3.Timescan
4.Kinetic
5.Quantitative
6.System Setup
7.Applications

Select Number:1

Quick Sample
Run Control
Figure 3.6 System Main Menu screen

Two functions, Quick Run and Sample Control, are available in this screen. Quick Run is a function the

12
 Metertech Inc.

same as described before by using the QUICKRUN key. Sample Control will be functioning only when the
multi-cell holder or the sipper is used.

13
 Metertech Inc.

Chapter 4 SYSTEM SETUP

In System Main Menu, selecting item 6 will get into the System Setup Menu screen (Figure 4.1). In this
screen, users are allowed to alter several parameters described below. In this screen only one function,
Load B-Line, is available.

12:00:00 500.0nm 0.000A

System Setup Menu

1.Setup Clock
2.Setup Lamp
3.Setup Printer
4.Setup RS-232
5.Setup Hardware
6.Measure Bandwidth
7.Measure Base-line
8.Search Zero Position

Select Number:1

Load
B-Line
Figure 4.1 System Setup Menu screen

Load B-Line Will reload the results of Base-line measurement.

§4-1 System Setup Menu

Setup Clock To set values (Figure 4.2) for Month, Date, Year, Hour, Minute, and

12:00:00 500.0nm 0.000A

Clock Parameter Setup
1.Month :08
2.Date :04
3.Year :99
4.Hour :15
5.Minute :28
6.Second :00

Number from 01 to 12...

Print Prev
Data Screen
Figure 4.2 Clock Parameter Setup screen
14
 Metertech Inc.

Second as necessary.
Setup Lamp Use function keys to set up parameters for visible and UV lamps
(Figure 4.3). Switch UV is for switching ON/OFF of UV lamp. Reset
Vis Hr is for resetting the usage time of the tungsten lamp. Reset UV
Hr is for resetting the usage time of the UV lamp. Energy Vis is for

12:00:00 500.0nm 0.000A

Lamp Parameter Setup
1.Visible lamp status :ON
2.Visible lamp used hours :20
3.Visible lamp energy :100%
4.UV lamp status :ON
5.UV lamp used hours :20
6.UV lamp energy :100%

Switch Reset Reset Energy Energy

UV Vis Hr UV Hr Vis UV
Figure 4.3 Lamp Parameter Setup screen

measuring the power of the tungsten lamp. Energy UV is for

measuring the power of the UV lamp.
Setup Printer Four printers (SII DPU-41X, Epson 9/24 Pin, Epson Stylus and HP
Deskjet/Laserjet) can be selected through arrow keys as instructed
(Figure 4.4).

15
 Metertech Inc.

12:00:00 500.0nm 0.000A

Serial Port Parameter Setup
1.Baud rate :38400
2.Bit length :8
3.Parity check :NONE
4.Stop bit :1
5.Remote status :OFF

Press < > key to select...

Print Prev
Data Screen
Figure 4.5 Serial Port Parameter Setup screen

Setup RS-232 Parameters for RS-232 can be set up through instructions shown in
the screen (Figure 4.5).
Setup Hardware In this option, three adjustable items are shown (Figure 4.6). Use
arrow keys to move cursor to proper position and proceed as

12:00:00 500.0nm 0.000A

Accessory Parameter Setup
1.UV/VIS change wavelength :363.0
2.Switch to Single Cell :No
3.Scan base line at POWER ON :No

12:00:00 500.0nm 0.000A

Printer Parameter Setup
1.Printer Type :EPSON STYLUS

Available printer:
Number from 330.0 to 365.0...
1. SII DPU-411(or
PrintDPU-414) Prev
2. Epson 9/24 Data
Pin series Screen
3. Epson Stylus series
Figure
4. HP4.6 Accessory Parameter
Deskjet/Laserjet Setup screen
series

Press < > key to select...

Print Prev
Data Screen
Figure 4.4 Printer Parameter Setup screen

instructed at the bottom of this screen. UV/VIS Change Wavelength

16
 Metertech Inc.

12:00:00 BANDWIDTH 676.0nm

100.
0
%

50.0

0.0
636 656 676 nm
1=655.59nm
=657.44nm
Bandwidth=1.85nm
max=656.12nm
Measurement completed...
Print Prev
Data Screen
Figure 4.7 Bandwidth measurement screen

allows users to set switching point for UV/VIS lamps, while 363nm is
set as default. Switch to Single Cell allows users to switch
accessories between Single Cell and accessories; e.g., Multi-Cell or
Sipper. Scan Base Line at Power On allows users to determine
whether to execute base-line measurement or not right after turning
on the power.
Measure Bandwidth To perform bandwidth measurement by pressing READ. This
measurement detects spectrum on UV lamp and the result is used for
calibration (Figure 4.7). If the calibration for this instrument is
correct, there is a peak shown around wavelength 656.1nm and the
bandwidth is less than 2nm. After calibration, two functions are
available for users to choose: Print Data for printing the results, and
Prev Screen for going back to the System Setup Menu screen.
Measure Base line To execute the base-line measurement immediately after the ENTER
key is pressed.
Search Zero Position To perform Zero Position test for hardware.

17
 Metertech Inc.

Chapter 5 PHOTOMETRIC

This is a simple measurement mode with fixed wavelengths. According to the parameter setup, users can
repeat a measurement and a table of the measurement results will be shown. The results can be printed
with proper settings.

In System Main Menu, selecting item 1 will get into this mode. Instrument and analysis parameters are set
up in the PHOTOMETRIC Parameter Setup screen (Figure 5.1). Move the cursor to required parameter by
using up/down arrow keys and change values of parameters. Once parameters have been set, press F5
Next Screen to get into the PHOTOMETRIC array-typed screen. When samples are prepared ready, press
READ to proceed the measurement. Measuring results will be shown in the screen.

12:00:00 PHOTOMETRIC 500.0nm

Parameter Setup
1.Input the Number of :1
1 :500.0

2.Measure mode :ABS

Have unit(Yes/No)? :Yes
Unit :
Factor(default=1) :1.000
3.Cycle number :1
4.Cycle interval(sec) :0
5.Print every cycle :No

Number from 1 to 5...

Load Save Print Sample Next
Param Param Data Control Screen
Figure 5.1 Photometric Parameter Setup screen

Five functions are available in the first screen of this mode for users to select: Load Param, Save Param,
Print Data, Sample Control, and Next Screen.

§5-1 Photometric Parameter Setup

Input the Number of For entering the number of wavelength (1 to 5) and the value(s) of
wavelength to be used in the Photometric mode.
Measure Mode Selecting measurement results in ABS as for absorbance, in %T as for
transmittance, or in Conc as for concentration.
Cycle Number For entering the number of cycles (1 to 999) that a sample is required
to be measured. One sample being measured through all desired
wavelengths is called as one cycle.
Cycle Interval (sec) For entering the time (0 to 9999) between the end of previous cycle

18
 Metertech Inc.

and the beginning of next cycle.

Print Every Cycle Selecting Yes or No by pressing left/right arrow keys to print at the
end of every cycle.

§5-2 Photometric Function Structure

Photometric Load Param

Save Param
Print Data
Sample Control
Next Screen Print Data
Prev Screen

For functions not described below, please refer to General function description section.

§5-3 Basic Photometric Measurement

The following is a description of the basic procedure for carrying out measurement in the SP-8001
Photometric measurement mode. For example, five samples are ready to proceed photometric
measurement in 4 wavelengths: 400, 450, 500, and 550.

Step 1. In System Main Menu, select 1; then, press ENTER to get into the Parameter Setup screen.
Step 2. In Parameter Setup screen (Figure 5.2), key in the number of and the corresponding values
of . Each entry is followed by an ENTER key. Then go through other items (2 to 5) with proper
entries as instructed. If any setting needs correction, use up/down arrow keys to move the cursor
12:00:00 PHOTOMETRIC 500.0nm
Parameter Setup
1.Input the Number of :4
1 :400 2 :450
3 :500 4 :550
2.Measure mode :ABS
Have unit(Yes/No)? :Yes
Unit :
Factor(default=1) :1.000
3.Cycle number :1
4.Cycle interval(sec) :0
5.Print every cycle :No

Number from 1 to 5...

Load Save Print Sample Next
Param Param Data Control Screen
Figure 5.2 Setting of parameters
19
 Metertech Inc.

to the proper position. When all is done, press F5 Next Screen to next step.
Step 3. Get a sample ready and press READ. The reading of that sample will be displayed on the
screen. Repeat this step for other four samples. Readings of measurements will be displayed in
the screen (Figure 5.3).
Step 4. Data obtained in every measurement can be printed by pressing F3 Print Data. To get back to
Parameter Setup screen, press ESC or F5 Prev Screen.

12:00:00 PHOTOMETRIC 550.0nm

400.0 450.0 500.0 550.0

No ABS1 ABS2 ABS3 ABS4

1 0.855 1.223 1.501 1.802

2 0.855 1.224 1.502 1.803

3 0.854 1.223 1.502 1.801

4 0.855 1.224 1.503 1.803

5 0.854 1.223 1.501 1.802

Press READ when ready...

Print Prev
Data Screen
Figure 5.3 Photometric readings of samples

A very simple way to perform photometric measurement if users only need to get readings of samples in a
specific wavelength.
Step 1. In System Main Menu, press in the keyboard to get into the Lambda screen (Figure 5.4).
Step 2. Get the sample ready for measurement. In the Lambda screen, key in the value for wavelength

12:00:00 LAMBDA 500.0nm

: 500.0 nm

Data: 0.000 A

Press & keyin wavelength...

View Save Mode Sample Toggle
Data Data A/C/%T Control Digits
Figure 5.4 Lambda screen

20
 Metertech Inc.

and press ENTER. The reading of the measurement will show up in the screen.
Step 3. After the measurement, press F3 Mode A/C/%T can switch reading into different formats: A as
for Abs, C as for concentration, and %T as for transmittance. Value of factor will be asked for those
choosing Mode in C (Figure 5.5).
Step 4. When the reading is in ABS format, user may press F5 Toggle Digits to show five digits for more
precise reading (Figure 5.6). This function can only work under the ABS format.
Step 5. After the measurement, pressing F2 Save Data can save the current reading. The saved data

12:00:00 LAMBDA 500.0nm

: 500.0 nm

Data: 100.0 %T

Factor:1.000
From 1 to 9999 ...
Press & keyin wavelength...
View Save Mode Sample Toggle
Data Data A/C/%T Control Digits
Figure 5.5 Reading in %T format

will be displayed at the bottom of the screen (Figure 5.7). Up to 60 sets of data can be saved.

12:00:00 LAMBDA 500.0nm

: 500.0 nm

Data: 0.0000 A

Press & keyin wavelength...

View Save Mode Sample Toggle
Data Data A/C/%T Control Digits
Figure 5.6 Five-digit reading

21
 Metertech Inc.

Step 6. After data saved, pressing F1 View Data can get into the Data File List screen (Figure 5.8). It
will show the first 15 sets of data being saved. In this screen, functions F1 Prev Page is for viewing

12:00:00 LAMBDA 500.0nm

: 500.0 nm

Data: 0.000 A

Data01 500.0nm 0.000A

Press & keyin wavelength...
View Save Mode Sample Toggle
Data Data A/C/%T Control Digits
Figure 5.7 Saving data of measurement

previous page and F2 Next Page is for viewing next page. Pressing F3 Print Data will print out all
sets of data (60 sets the maximum). The saved data will be erased once users exit from this mode.

12:00:00 LAMBDA 600.0nm

Data File List
--------------------------------------
Data01 500.0nm 0.000A
Data02 500.0nm 100.0%T
Data03 400.0nm 1.200A
Data04 500.0nm 72.0%T
Data05 600.0nm 0.000A

Prev Next Print Prev

Page Page Data Screen
Figure 5.8 Data File List screen

22
 Metertech Inc.

Chapter 6 SPECTRUM

This is the mode in which spectral measurement is performed. Measured spectra can be saved to memory
and be subjected to various data manipulation described in details below. The measured spectra can be
zoomed by changing the interested range. Interested point of the spectra can be tracked manually and the
corresponding data will be shown in the screen. Peaks or valleys of measured spectra can be automatically
picked up and corresponding data can be shown in the screen. The number of interested peaks or valleys
can be changed in the same spectra. Multiple spectra can be viewed in the same graph for comparison
purpose.

In System Main Menu, selecting item 2 will get into this mode. Instrument and analysis parameters are set
up in the SPECTRUM Parameter Setup screen (Figure 6.1). Move the cursor to required parameter by using
arrow keys and change values of parameters as needed. Once parameters have been set, press F5 Next
Screen to get into the SPECTRUM measuring graph screen. When samples are prepared ready, press READ
to proceed the measurement. Measuring results will be shown in the screen.
Five functions are available in the first screen of this mode for users to select: Load Param, Save Param,
12:00:00 SPECTRUM 500.0nm
Parameter Setup
1.Start wavelength(nm) :400.0
2.Stop wavelength(nm) :800.0
3.Measure mode :ABS
4.Low value(ABS) :-0.30
5.High value(ABS) :3.00
6.Scan speed(nm/min) :1000
7.Overlay screen :Yes

Number from 190 to 1098...

Load Save Print Sample Next
Param Param Data Control Screen
Figure 6.1 Spectrum Parameter Setup screen

Print Data, Sample Control, and View Graph.

§6-1 Spectrum Parameter Setup

Start Wavelength (nm) For entering the Start Wavelength in Spectrum mode between 190nm
and 1098nm.
Stop Wavelength (nm) For entering the Stop Wavelength in Spectrum mode between 192nm
and 1100nm. The Stop Wavelength must be at least 2nm greater
than the Start Wavelength.
23
 Metertech Inc.

Measure Mode Selecting measurement results in ABS as for absorbance or in %T as

for transmittance.
Low Value For entering the lower limit of measurement result between –0.3 and
2.99 for ABS and between 0 and 119.9 for %T, respectively.
High Value For entering the upper limit of measurement result between –0.29
and 3.00 for ABS and between 0.1 and 120.0 for %T, respectively.
The High Value must be at least 0.1 greater than the Low Value
regardless the mode.
Scan Speed (nm/min) For entering the Scan Speed in Spectrum mode between 100 and
5000 (nm/min).
Overlay Screen Selecting Yes or No to overlay the current measurement with previous
measurements. Note that only data for the current measurement
can be manipulated.

§6-2 Spectrum Function Structure

Load Data
Spectrum Load Param
Save Data
Save Param
Access Data Print Data
Print Data Prev Page
Sample Control Smooth Data View Data Next Page
Next Screen Manipulate Zoom Data Save Data Print Data
Show Track Print Data Prev Screen
Peak Valley View Data Prev Page
Original Search Peak Next Page
Clear Graph Search Valley Print Data
Prev Screen Less Point Prev Screen
More Point

For functions not described below, please refer to General function description section.

Access Data To load existing data from memory or to save data of current
measurement into memory.
Smooth Data To smooth the data of current measurement in the screen. Note that
only the current measurement will go through this function and will
be shown after completion of this function.
Zoom Data To zoom in the selected range of data in the current screen. Key in
proper values at blinking cursor followed by ENTER keys and the
selected range of data can be zoomed.
Show Track A vertical dotted line will show up as a tracking cursor. Left/right

24
 Metertech Inc.

arrow keys can be used to move the cursor and the corresponding
data will be displayed at the upper right of the screen. In this
function, users press Save Data to temporarily save data of the points
being tracked. Those data can only be used as pressing F1 View
Data or F3 Print Data and will be vanished once jumping out from this
function.
View Data To review data being saved under function Show Track or being found
under function Peak Valley.
Prev Page To review data in the previous page. Only 15 sets of data can be
displayed in one screen.
Next Page To review data in the next page. Only 15 sets of data can be
displayed in one screen.
Peak Valley For users to find peaks or valleys in the current graph.
Search Peak To search peaks in the current graph. Tiny squares show in the
graph to represent the peaks found under this function. The
corresponding data can be viewed under View Data function.
Search Valley To search valleys in the current graph. Tiny squares show in the
graph to represent the valleys found under this function. The
corresponding data can be viewed under View Data function.
Less Point To reduce the number of peaks or valleys found in the current graph.
The corresponding data can be viewed under View Data function.
More Point To find more peaks or valleys in the current graph. The
corresponding data can be viewed under View Data function.
Original To redraw the graph from data obtained in the current measurement.

§6-3 Basic Spectrum Measurement

The following is a description of the basic procedure for carrying out measurement in the SP-8001 Spectrum
measurement mode. For example, a spectrum of sample is to be measured from 400nm to 800nm and the
results should be bounded between 0 and 1.5Abs. Scanning speed is set at 1000nm/min.

Step 1. In System Main Menu, select 2; then, press ENTER to get into the Parameter Setup screen.
Step 2. In Parameter Setup, key in desired values or select proper choice in those 7 items step by step,
followed by an ENTER (Figure 6.2). If any setting needs correction, use up/down arrow keys to
move the cursor to the proper position. When all is done, press F5 Next Screen to get into the
Spectrum screen.

25
 Metertech Inc.

12:00:00 SPECTRUM 500.0nm

Parameter Setup
1.Start wavelength(nm) :400.0
2.Stop wavelength(nm) :800.0
3.Measure mode :ABS
4.Low value(ABS) :0.00
5.High value(ABS) :1.50
6.Scan speed(nm/min) :1000
7.Overlay screen :Yes

Number from 190 to 1098...

Load Save Print Sample Next
Param Param Data Control Screen
Figure 6.2 Setting of parameters

Step 3. Make the sample ready and press READ. The measurement result will be displayed in the
Spectrum screen (Figure 6.3) in real-time mode. The upper right corner of the screen will show the
current wavelength and the current reading.
Step 4. When parameter Overlay Screen is set Yes, every measurement result will be displayed on the
Spectrum screen. Pressing F3 Clear Graph can clear all graphs in the screen.

12:00:00 SPECTRUM 800.0nm

1.50

ABS

0.75

0.00
400 nm 605 800

Press READ when ready...

Access Mani- Clear Prev
Data pulate Graph Screen
Figure 6.3 Measuring result in Spectrum screen

Step 5. If users want to manipulate data of results, press F2 Manipulate to get into another layer of
function keys (Figure 6.4). Then, press F1 Smooth Data and it will smooth the current graph to
reduce the effect of noise. To zoom data, go to Step 6; to show track, go to Step 7; and to search
peaks and valleys of the graph, go to Step 9.

26
 Metertech Inc.

12:00:00 SPECTRUM 800.0nm

1.50

ABS

0.75

0.00
400 nm 605 800

Press READ when ready...

Smooth Zoom Show Peak Origi-
Data Data Track Valley nal
Figure 6.4 Functions for manipulating graph

Step 6. If users want to view part of the graph, function Zoom Data is designed for this purpose. Press
F2 Zoom Data, then cursor will sequentially move through 1.50 (High Value), 0.00 (Low Value) of the
vertical axis and 400 (Start Wavelength), 800 (Stop Wavelength) of the horizontal axis in the graph.
Key in the desired value followed by an ENTER key, cursor will move on to the next input position.
After all inputs done, the instrument will display a new graph subject to the newly defined
parameters (Figure 6.5).

12:00:00 SPECTRUM 800.0nm

1.50

ABS

0.75

0.00
400 nm 500 600

Press hot key to process...

Smooth Zoom Show Peak Origi-
Data Data Track Valley nal
Figure 6.5 Graph with zoomed data

Step 7. If users want to trace readings of measurement result, function Show Track is designed for this
purpose. Pressing F3 Show Track will make a dotted vertical line showing up in the graph (Figure
6.6). Use left/right arrow keys to move the dotted line one pixel in the corresponding direction.
Use up/down arrow keys to move dotted line ten pixels in right and left directions respectively.

27
 Metertech Inc.

12:00:00 SPECTRUM 533.2nm

1.50

ABS

0.75

0.00
400 nm 500 600

Press < > key to move...

View Save Print
Data Data Data
Figure 6.6 Graph under Show Track function

During the tracing process, corresponding values for traced point will be displayed at the up right
corner in the screen.
Step 8. Under Show Track mode, data of the traced point can be saved by pressing F2 Save Data. And
a number will be assigned automatically to that traced point. The number will be displayed at the
lower right corner in the screen (Figure 6.7). Total 60 sets of data can be saved. In this mode,
pressing F3 Print Data will print out this screen with the dotted line. Pressing F1 View Data will
show the Data File List screen that displays all saved data in the Show Track mode.

12:00:00 SPECTRUM 562.4nm

1.50

ABS

0.75

0.00
400 nm 500 600
Data No.=05
Press < > key to move...
View Save Print
Data Data Data
Figure 6.7 Saving data in Show Track mode

Step 9. In the Spectrum screen, pressing function Manipulate and then function Peak Valley will get into
the mode that peaks and valleys of the graph can be picked up automatically. In this searching
mode, pressing F2 Search Peak will mark all peaks in the graph (Figure 6.8). Functions F4 and F5
will let users to adjust the number of points being picked. Detailed data of those points can be
28
 Metertech Inc.

12:00:00 SPECTRUM 800.0nm

1.50

ABS

0.75

0.00
400 nm 605 800

Press hot key to processing...

View Search Search Less More
Data Peak Valley Point Point
Figure 6.8 Searching peaks of the spectrum

viewed by pressing F1 View Data to see the wavelength and the corresponding reading (Figure 6.9).

12:00:00 SPECTRUM 800.0nm

Data File List
--------------------------------------
Data01 413.2nm 0.344A
Data02 498.8nm 1.415A
Data03 587.1nm 0.352A
Data04 714.3nm 1.065A
Data05 787.5nm 0.534A

Prev Next Print Prev

Page Page Data Screen
Figure 6.9 Data File List for peaks

29
 Metertech Inc.

Chapter 7 TIMESCAN

This is the mode in which absorbance versus time measurement of a sample is performed for the accurate
study of continuous changes in reaction rates. Delay Time can be set before the start of Scan Time.
Results can be shown in a graph and also be manipulated in various ways as needed.

In System Main Menu, selecting item 3 will get into this mode. Instrument and analysis parameters are set
up in the TIMESCAN Parameter Setup screen (Figure 7.1). Move the cursor to required parameter by using
arrow keys and change values of parameters. Once parameters have been set, press function Next Screen
to get into the TIMESCAN measuring graph screen. When samples are prepared ready, press READ to
proceed the measurement. Measuring results will be shown in the screen.

12:00:00 TIMESCAN 500.0nm

Parameter Setup
1.Wavelength(nm) :500.0
2.Scan time(sec) :30
3.Delay time(sec) :0
4.Measure mode :ABS
5.Low value(ABS) :-0.30
6.High value(ABS) :3.00
7.Overlay screen :Yes

Number from 190 to 1100…

Load Save Print Sample Next
Param Param Data Control Screen
Figure 7.1 TimeScan Parameter Setup screen

Five functions are available in the first screen of this mode for users to select: Load Param, Save Param,
Print Data, Sample Control, and Next Screen.

§7-1 TimeScan Parameter Setup

Wavelength (nm) For entering the value of wavelength to be used in the TimeScan
mode. The value should range from 190 to 1100.
Scan Time (sec) For setting the time in second over which the sample will be
measured. The value should range from 5 to 3000.
Delay Time (sec) For setting the time in second between pressing READ and the start
of the measurement. The value should range from 0 to 50.
Measure Mode Selecting measurement results in ABS as for absorbance or in %T as
for transmittance.

30
 Metertech Inc.

Low Value For entering the lower limit of measurement result between –0.3 and
2.99 for ABS and between 0 and 119.9 for %T, respectively.
High Value For entering the upper limit of measurement result between –0.29
and 3.00 for ABS and between 0.1 and 120.0 for %T, respectively.
The High Value must be at least 0.1 greater than the Low Value
regardless the mode.
Overlay Screen Selecting Yes or No to overlay the current measurement with previous
measurements. Note that only data for the current measurement
can be manipulated.

§7-2 TimeScan Function Structure

TimeScan Load Param

Load Data
Save Param
Save Data
Print Data
Access Data Print Data
Sample Control Prev Page
Smooth Data View Data Next Page
Next Screen Manipulate Zoom Data Save Data Print Data
Show Track Print Data Prev Screen
Peak Valley View Data Prev Page
Original Search Peak Next Page
Clear Graph Search Valley Print Data
Prev Screen Less Point Prev Screen
More Point

For functions not described below, please refer to General Function Description section.

Access Data To load existing data from memory or to save data of current
measurement into memory.
Smooth Data To smooth the data of current measurement in the screen. Note that
only the current measurement will go through this function and will
be shown after completion of this function.
Zoom Data To zoom in the selected range of data in the current screen. Key in
proper values at blinking cursor followed by ENTER keys and the
selected range of data can be zoomed.
Show Track A vertical dotted line will show up as a tracking cursor. Right/left
arrow keys can be used to move the cursor and the corresponding
data will be displayed at the upper right of the screen. In this
function, users press Save Data to temporarily save data of the points
31
 Metertech Inc.

being tracked. Those data can only be used as View Data or Print
Data being pressed and will be vanished once jumping out from this
function.
View Data To review data being saved under function Show Track or being found
under function Peak Valley.
Prev Page To review data in the previous page. Only 15 sets of data can be
displayed in one screen.
Next Page To review data in the next page. Only 15 sets of data can be
displayed in one screen.
Peak Valley For users to find peaks or valleys in the current graph.
Original To redraw the graph from data obtained in the current measurement.

§7-3 Basic TimeScan Measurement

The following is a description of the basic procedure for carrying out measurement in the SP-8001 TimeScan
measurement mode. For example, users want to know the measurement change of a sample in a time
span of 300 seconds at wavelength 500nm.

Step 1. In System Main Menu, select 3; then, press ENTER to get into the Parameter Setup screen.
Step 2. In Parameter Setup screen (Figure 7.2), key in required values in the corresponding parameters.
Each entry is followed by an ENTER key. If any setting needs correction, use up/down arrow keys to
move the cursor to the proper position. When all is done, press F5 Next Screen to next step.
Step 3. Make the sample ready and press READ. The measurement result will be displayed in the
TimeScan screen (Figure 7.3) in real-time mode.

12:00:00 TIMESCAN 500.0nm

Parameter Setup
1.Wavelength(nm) :500.0
2.Scan time(sec) :300
3.Delay time(sec) :0
4.Measure mode :%T
5.Low value(ABS) :99.0
6.High value(ABS) :101.0
7.Overlay screen :Yes

Number from 190 to 1100...

Load Save Print Sample Next
Param Param Data Control Screen
Figure 7.2 Setting of parameters
32
 Metertech Inc.

Step 4. After the measurement, functions can be selected in the same way as in Spectrum mode.

12:00:00 TIMESCAN 500.0nm

101.
0
%T

100.
0

99.0
0 sec 150 300

Press READ when ready...

Access Mani- Clear Prev
Data pulate Graph Screen
Figure 7.3 Measurement in TimeScan screen

33
 Metertech Inc.

Chapter 8 KINETIC

In this mode, the time-dependent changes in absorbance (ABS) at a fixed wavelength are measured and
recorded. A typical application in this mode is the measurement of an enzyme activity. The time changes
in absorbance will be displayed on screen and the absorbance can be found at each sampling interval, set
according to Lag Time plus with Rate Time. The segment after measurement has started which is not
subject to activity computation is set as Lag Time. The amount of change in absorbance is calculated as
Rate Time that is evenly divided. The rate of change in absorbance is calculated with the least square
method and can be recalculated for different segments by resetting the Lag Time and Rate Time for the
same graph.

In System Main Menu, selecting item 4 will get into this mode. Instrument and analysis parameters are set
up in the KINETIC Parameter Setup screen (Figure 8.1). Move the cursor to required parameter by using
arrow keys and change values of parameters. Once parameters have been set, press function View Graph
to get into the KINETIC measuring graph screen. When samples are prepared ready, press READ to
proceed the measurement. Measuring results will be shown in the screen.

12:00:00 KINETIC 500.0nm 0.000A

Parameter Setup
1.Wavelength(nm) :500.0
2.Sampling Number :5
3.Lag Time(sec) :2
4.Rate Time(sec) :12
5.Delay Time(sec) :0
6.Have unit(Yes/No)? :No

7.Factor(default=1) :1.0
8.Low Value(ABS) :-0.30
9.High Value(ABS) :3.00
10.Measure Temperature :None
11.Overlay Screen :Yes
12.Print every cycle :No

Number from 2 to 99…

Load Save Print Sample Next
Param Param Data Control Screen
Figure 8.1 Kinetic Parameter Setup screen

Five functions are available in the first screen of this mode for users to select: Load Param, Save Param,
Print Data, Sample Control, and Next Screen.

34
 Metertech Inc.

§8-1 Kinetic Parameter Setup

Wavelength (nm) For entering the value of wavelength to be used in the Kinetic mode.
The value should range from 190 to 1100.
Sampling number For entering the sampling number of a measurement in the Kinetic
mode. This number of data is used for the activity calculations.
The value should range from 2 to 20.
Lag Time (sec) For setting the time in second from the start of measurement during
which data will not be acquired for activity calculations. The value
should range from 2 to 9999.
Rate Time (sec) For setting the time in second after the Lag Time during which data
will be acquired for activity calculations. The value should range
from 3 to 9999.
Delay Time (sec) For setting the time in second between pressing READ and the start
of the measurement. The value should range from 0 to 50.
Have Unit (Yes/No)? Selecting the unit option for measurement result. When Yes is
selected, pick the right unit out of the thirteen build-in units in table.
Factor (default=1) For entering the value of Factor. This factor is used for converting
the absorbance rate of change into the activity value.
Activity Value = Factor X Abs Rate of Change ( ABS/min)
The value should range from 0 to 9999.
Low Value (ABS) For entering the lower limit of measurement result between –0.3 and
2.99 for ABS.
High Value (ABS) For entering the upper limit of measurement result between –0.29
and 3.00 for ABS. The High Value must be at least 0.1 greater than
the Low Value.
Measure Temperature Selecting temperature control over the measurement. Four
selections are available: None, 25, 30, and 37 in Celsius. It can be
functional only when a temperature controlled accessory being
installed.
Overlay Screen Selecting Yes or No to overlay the current measurement with previous
measurements. Note that only data for the current measurement
can be manipulated.
Print Every Cycle Selecting Yes or No to print at the end of every cycle.

35
 Metertech Inc.

§8-2 Kinetic Function Structure

Kinetic Load Param

Save Param Change Scale
Print Data Repeat Measure
Sample Control Print Data
Next Screen Data List Sample Control
Prev Screen
Manipulate Linear Fit
Clear Graph Show Track Print Data
Print Data Original
Prev Screen

Data List Switch to the KINETIC Data File List screen for users to review data
obtained in previous measurements after setup of parameters.
Change Scale For users to perform measurements with different values of Lag Time
and Rate Time. Pressing this function key will switch input fields for
users to key in values. Measurement results can still been shown in
the same KINETIC Data File List screen.
Repeat Measure To repeat the measurement once under the current parameter setup.
Linear Fit To perform linear fitting for the current measurement.
Show Track A vertical dotted line will show up as a tracking cursor. Left/right
arrow keys can be used to move the cursor and the corresponding
data will be displayed at the upper right of the screen. In this
function, users press F2 Save Data to save data of the points being
tracked. Those data can only be used as F1 View Data or F3 Print
Data being pressed and will be vanished once jumping out from this
function.
View Data To review data being saved under function Show Track or being found
under function Peak Valley.
Prev Page To review data in the previous page. Only 15 sets of data can be
displayed in one screen.
Next Page To review data in the next page. Only 15 sets of data can be
displayed in one screen.
Original To redraw the graph from data obtained in the current measurement.
§8-3 Basic Kinetic Measurement

The following is a description of the basic procedure for carrying out measurement in the SP-8001 Kinetic
measurement mode. For example, users want to know the reaction of a reagent in a time span of 60
36
 Metertech Inc.

seconds after 60-second lag time by sampling 5 times at wavelength 405nm, assuming that factor is 3253
and unit is set as U/L.

Step 1. In System Main Menu, select 4; then, press ENTER to get into the Parameter Setup screen.
Step 2. In Parameter Setup screen (Figure 8.2), key in required values in the corresponding parameters.
Each entry is followed by an ENTER. If any setting needs correction, use up/down arrow keys to
move the cursor to the proper position. In item 6, select Yes followed by an ENTER key and in the
unit list, choose 3 (U/L) followed by an ENTER key. When all is done, press F5 Next Screen to next
step.
Step 3. Make the sample ready and press READ. The measurement result will be displayed in the
Kinetic screen (Figure 8.3) in real-time mode. The measuring curve shows the measurements taken
by the instrument. After completion of the measurement, the instrument will take the pre-set
number of sampling measurements for calculation to perform linear fitting. The small blocks shown
on the curve represent sample measurements during the rate time. Those blocks are used for linear

12:00:00 KINETIC 500.0nm 0.000A

Parameter Setup

1.Sampling Number :5
2.Wavelength(nm) :405.0
3.Lag Time(sec) :60
4.Rate Time(sec) :60
5.Delay Time(sec) :0
6.Have unit(Yes/No)? :Yes
7.Factor(default=1) :3253
8.Low Value(ABS) :-0.30
9.High Value(ABS) :1.00
10.Measure Temperature :None
11.Overlay Screen :Yes
12.Print every cycle :No

Number from 2 to 99...

Load Save Print Sample Next
Param Param Data Control Screen
Figure 8.2 Setting of parameters

fitting and the equation of regression is shown at the bottom of this screen. T shows the rate
time. ABS T shows the absorbance rate of change. C/ T shows the concentration rate of
change after conversion by using the factor. In this screen users can to view the data of sampling
point by pressing F1 Data List. Pressing F2 Manipulate will go to next step.
Step 4. In this screen, press F2 Linear Fit to get the regression line and it will show on the screen
(Figure 8.4). Here, only the regression line and the sampling blocks will show up. Users can also
switch back to the original measuring curve by pressing F5 Original.

37
 Metertech Inc.

12:00:00 KINETIC 405.0nm 0.540A

1.00
ABS

0.35

-0.3
0
60 90 120 sec
ΔT=60.0 Factor=3253.000
ΔABS/ΔT=0.11308( ABS/min)
ΔC /ΔT=367.86414( U/L/min)
ABS =0.00188T + 0.27194
Press READ when ready…
Data Mani- Clear Print Prev
List pulate Graph Data Screen
Figure 8.3 Kinetic measuring screen

12:00:00 KINETIC 405.0nm 0.540A

1.00
ABS

0.35

-0.3
0
60 90 120 sec
ΔT=60.0 Factor=3253.000
ΔABS/ΔT=0.11308( ABS/min)
ΔC /ΔT=367.86414( U/L/min)
ABS =0.00188T + 0.27194
Press hot key to processing…
Linear Show Origi-
Fit Track nal
Figure 8.4 Kinetic Manipulate screen

Step 5. In the Kinetic Manipulate screen, users can press F3 Show Track to view data of the measuring
curve or the regression line by moving the dotted tracing line.

38
 Metertech Inc.

Chapter 9 QUANTITATIVE

This is the mode in which the concentration of an unknown sample under a specific wavelength can be
determined by creating a calibration curve from standard samples. Four methods for curve fitting can be
selected: straight line with or without passing through origin and 2nd order polynomial with or without
passing through origin.

In System Main Menu, selecting item 5 will get into this mode. Instrument and analysis parameters are set
up in the QUANTITATIVE Parameter Setup screen (Figure 9.1). Move the cursor to required parameter by
using arrow keys and change setting of parameters. Once parameters have been set, press F5 Next Screen
to get into the QUANTITATIVE Standard Table screen. When standard samples are prepared ready, press
READ to proceed the measurement. Once the standard table has been built up, press function Manipulate
to move on. Users then can view the corresponding equation or measure new samples as needed.
Measuring results will be shown in the Measure Sample screen.

12:00:00 QUANTITATIVE 500.0nm 0.000A

Parameter Setup
1.Wavelength(nm) :500.0
2.Standard number(Conc.) :3
S 1:0.0 S 2 :0.0
S 3:0.0

3.Have Unit(Yes/No) :No

4.Method :1’ord.(Origin)
5.Keyin ABS :No
6.Print every cycle :No

Number from 190 to 1100...

Load Save Print Sample Next
Param Param Data Control Screen
Figure 9.1 Quantitative Parameter Setup screen

Five functions are available in the first screen of this mode for users to select: Load Param, Save Param,
Print Data, Sample Control, and Next Screen.

§9-1 Quantitative Parameter Setup

Wavelength (nm) For entering the value of wavelength to be used in the Quantitative
mode. The value should range from 190 to 1100.
Standard Number (Conc.) For entering the number of standard samples used in the process.
The value should range from 1 to 10. Concentration for each
standard sample can also be entered in the corresponding fields.

39
 Metertech Inc.

Have Unit (Yes/No) Selecting the unit option for measurement results. When Yes is
selected, pick the right unit out of the thirteen build-in units in table.
Method Four methods of data fitting are available: 1’ord.(Origin) for straight
line through origin, 1’ord.(No Org) for straight line not passing
through origin, 2’ord.(Origin) for 2nd ordered polynomial through
origin, and 2’ord.(No Org) for 2nd ordered polynomial without passing
through origin.
Keyin ABS Selecting Yes for users to manually key in values of ABS. Selecting
No for instrument to read ABS values of standard samples
automatically. When first time get into the QUANTITATIVE Standard
Table screen, press READ to initiate this procedure.
Print Every Cycle Selecting Yes or No to print at the end of every measurement.

§9-2 Quantitative Function Structure

Quantitative Load Param

Load Data
Save Param
Save Data
Print Data Keyin Factor
Access Data Print Data
Sample Control Print Data
View Equati Prev Screen
Next Screen Manipulate Sample Measure Print Data
Sample Control
Prev Screen
View Curve Print Data
Prev Screen Prev Screen Prev Screen

For functions not described below, please refer to General function description section.

Access Data To load existing data from memory or to save data of current status
into memory. Here data refers to those factors of fitted equation
with parameter setup in the Quantitative mode. Use ESC key to get
back to the function level.
View Equati To view the fitted equation and associated factors under determined
fitting method.
View Curve To view the fitted equation in graph representation with tiny squares
representing data for standard samples.
Keyin Factor For entering factor values for the fitted equation.
Sample Measure To get into the QUANTITATIVE Measure Sample screen in order to
40
 Metertech Inc.

read samples whenever ready.

§9-3 Basic Quantitative Measurement

The following is a description of the basic procedure for carrying out measurement in the SP-8001
Quantitative measurement mode. In this mode, users use standard samples to determine unknown
samples. For example, there are three standard samples with known concentration and absorbance. If
the absorbance values are unknown, users can also use the SP-8001 to find out the corresponding
absorbance values. Using those data, the relationship between concentration and absorbance can be build
up through curve fitting technique. Once the equation being constructed, users can use it to determine
concentrations of unknown samples by reading the absorbance from the instrument.

Step 1. In System Main Menu, select 5; then, press ENTER to get into the Parameter Setup screen.
Step 2. In Parameter Setup screen (Figure 9.2), key in required values in the corresponding parameters.
Each entry is followed by an ENTER. If any setting needs correction, use Up/Down arrow keys to
move the cursor to the proper position. Here, set wavelength at 500 nm. In item 2, input 3
followed by an ENTER key. Then, in the corresponding fields key in concentration values followed by
ENTER keys. Select U/L as the unit in item 3. Select 1'ord.(Origin) in item 4 Method. In item 5,
set Yes for later keying in absorbance value manually. After all parameters set up, press F5 Next
Screen to next step.
Step 3. In the Quantitative Standard Table screen (Figure 9.3), press READ to start to key in absorbance
value for corresponding concentration. After finishing the key-in operation, press ESC to exit. Now,
12:00:00 QUANTITATIVE 500.0nm 0.000A
Parameter Setup
1.Wavelength(nm) :500.0
2.Standard number(Conc.) :3
S 1:1.0 S 2 :2.0
S 3:3.0

3.Have Unit(Yes/No) :Yes

4.Method :1’ord.(Origin)
5.Keyin ABS :Yes
6.Print every cycle :No

Number from 190 to 1100...

Load Save Print Sample Next
Param Param Data Control Screen
Figure 9.2 Setting of parameters

press F2 Manipulate to go to next step. After F2 Manipulate being pressed, the SP-8001 will proceed
calculation for the input data and an equation describing the relation of input data will be build up.

41
 Metertech Inc.

Step 4. Here, different function keys will show in the bottom of this screen (figure 9.4). Press F2
12:00:00 QUANTITATIVE 500.0nm
Standard Table
--------------------------------------
Sample No. Conc.( G/L) ABS
1 1.0000 0.1
2 2.0000 0.2
3 3.0000

Number from –0.3 to 3.0...

Access Mani-Re Prev
Data pulate Screen
Figure 9.3 Standard Table screen

Sample Measure to start measurement for unknown samples and it is described in step 5. Pressing
F1 View Equati will allow users to view details of the fitted curve and it is described in step 6.
Step 5. In the Quantitative Measure Sample screen (Figure 9.5), get the unknown sample ready and
press READ, then the SP-8001 will measure the absorbance of the sample and calculate the

12:00:00 QUANTITATIVE 500.0nm

Standard Table
--------------------------------------
Sample No. Conc.( G/L) ABS
1 1.0000 0.1
2 2.0000 0.2
3 3.0000 0.3

Press hot key to process...

View Sample View Prev
Equati Measure Curve Screen
Figure 9.4 More functions in Standard Table

corresponding concentration by using the equation obtained before. Results will be shown in the
screen and can also be printed by pressing F3 Print Data.
Step 6. In the Quantitative Linear Fitting screen (Figure 9.6), details of the fitted curve will be shown.
The equation describes the relation between concentration and absorbance value. K1 and K0 are
the coefficients in the equation and depend on the selected method of curve fitting. r2 shows the
correlation coefficient for the fitted equation.
42
 Metertech Inc.

12:00:00 QUANTITATIVE 500.0nm

Measure Sample
--------------------------------------
Sample No. ABS Conc.( G/L)
1 0.521 5.2100
2 1.137 11.370
3 2.325 23.250

Press READ when ready...

Print Sample Prev
Data Control Screen
Figure 9.5 Quantitative Measure Sample screen

Chapter 10 ACCESSORIES

12:00:00 QUANTITATIVE 500.0nm

Linear Fitting
--------------------------------------

Conc. = K1*ABS + K0
K1 = 10.00000
K0 = 0.00000

r² = 1.00000

Keyin Print Prev

Factor Data Screen
Figure 9.6 quantitative Linear Fitting screen

Metertech. Inc. designed several optional accessories to meet users’ specific purposes while the Single cell
holder is a standard accessory. When an optional accessory is to be used, users should turn off the
instrument first and then do the hardware installation as well as the cable connection. The SP-8001 will
automatically detect attached accessory. When the instrument is turned on, the detected accessories will
be shown during initialization.

After initialization, pressing function Sample Control will get into screens to set up the parameters of
accessories. Whenever function Sample Control shows up in various screen, it can be pressed to run the
accessory accordingly. A function named Prev Screen will close up the operation of accessories and back to
43
 Metertech Inc.

the previous screen. The optional accessory can be disabled in the System Setup by selecting item 5 Setup
Hardware; however, it has to be detected during the initialization process. Hot-plug is not recommended
and it may cause damage

§10-1 Multi-Cell Holder

The Multi-Cell Holder is an optional accessory that enables up to 6 cells to be presented for measurement
sequentially. Put the Multi-Cell Holder into the sample compartment and fix it with 4 screws as shown in
Figure 10.1. Plug the 25-pin male connector into the corresponding female connector in the sample
compartment. Turn on the instrument and it will be automatically detected.

Slider Cell holder

25-pin male connector

Figure 10.1 Multi-Cell Holder

When the Multi-Cell Holder is installed, pressing Read will perform measurements of all cells being set
according to Drive Cell Number. Also, at the right bottom corner of every screen, a note shows the current
Cell number that is in the measurement position.

A Multi-Cell Parameter Setup screen (Figure 10.2) will show up when function Sample Control being pressed.
Detailed settings and respective functions will be described below.

44
 Metertech Inc.

12:00:00 500.0nm 0.000A

Multi-Cell Parameter Setup
1.Driver cell Number(1-6) :2
2.Reagent blank corr.(cell 1) :Yes

Cell from 1 to 6... Cell=6

Cell Cell Cell Prev
Home Standby Blank Screen
Figure 10.2 Multi-Cell Parameter Setup screen

Driver Cell Number (1-6) For entering the number of cells to be measured. The value should
range from 1 to 6.
Reagent Blank Corr. (Cell 1) Selecting Yes to confirm that Cell 1 is used as Blank and No for using
base-line as blank.

Three functions are available in this screen: Cell Home, Cell Standby, and Cell Blank.

Cell Home This will make the Multi-Cell Holder in such a position that Cell 1 is
ready for the measurement.
Cell Standby This will make the Multi-Cell Holder in such a position that it is
convenient for users to exchange cells.
Cell Blank (not available for the time being)

45
 Metertech Inc.

§10-2 Sipper

The Sipper (Figure 10.3) is an optional accessory that enables samples to be drawn into a flow cell for
automatic measurement. After the measurement is complete the sample may be sent to a waste bottle or
returned to its original vessel.

From cell

To cell

25-pin male connector

Sample inlet

To waste bottle

Figure 10.3 Sipper

A Sipper Parameter Setup screen (Figure 10.4) will show up when function Sample Control being pressed.
Detailed settings and respective functions will be described below:

12:00:00 500.0nm 0.000A

Sipper Parameter Setup
1.Pump speed :Medium
2.Pre air purge time(sec) :2.5
3.Pre sipper time(sec) :2.5
4.Air purge time(sec) :3.5
5.Sipper time(sec) :3
6.Dwell time(sec) :1

Press < > key to select...

Wash Sipper Prev
Cell Test Screen
Figure 10.4 Sipper Parameter Setup screen

Pump Speed Three speeds: Slow, Medium, and Fast can be chosen by using arrow
keys to set up the speed of peristaltic pump. This setting only
46
 Metertech Inc.

applies on the sipping operation for Sipper Time.

Pre Air Purge Time (sec) For entering the value of time during which Sipper will do the air
purge operation for the previous sample. The value should range
from 0 to 99.9.
Pre Sipper Time (sec) For entering the value of time during which Sipper will draw the
residual of previous sample passing through the flow cell. The value
should range from 0 to 99.9.
Air Purge Time (sec) For entering the value of time during which Sipper will do the air
purge operation as required. This usually applies to those samples
that need segmentation by air. The value should range from 0 to
99.9.
Sipper Time (sec) For entering the value of time for the sipping operation. Samples will
be drawn into the flow cell for measurement essentially in this
operation of the Sipper. The value should range from 0 to 99.9.
Dwell Time (sec) For entering the value of time after the sipping operation. This will
allow samples getting into a stable condition for measurement. The
value should range from 0 to 99.9.

Two functions are available in this screen: Wash Cell and Sipper Test.

Wash Cell To drive peristaltic pump running for about 15 seconds to wash flow
cell whenever needed.
Sipper Test To execute one cycle of all operations sequentially according to the
settings.

47
 Metertech Inc.

Chapter 11 MAINTENANCE

The information given in this section deals only with those parts of maintenance or service which can be
safely carried out by users. Work other than those detailed later should be carried out by a trained service
engineer.

Poor instrument performance or failure can usually be attributed to simple failure of the tungsten lamp.
Therefore, as detailed below, replace the old one with a spare lamp provided before seeking further
assistance.

§11-1 Routine Maintenance

Very little maintenance is required to keep the instrument in good working condition. The interior should be
kept as dust free as possible and the sample compartment cleaned regularly; wipe off spilt chemicals
immediately.

CAUTION: Do not allow moisture to leak into the instrument.

The exterior of the instrument can be cleaned periodically as follows:

♦ SWITCH OFF first the instrument and disconnect from the mains supply.
♦ Using a lint free cloth dampened with a weak solution of detergent and water, wipe the exterior surface
of the instrument as necessary.
♦ Wipe over with a soft cloth dampened with clean water.
♦ Dry the surface with another soft cloth.

48
 Metertech Inc.

§11-2 Replacement of Lamps

This instrument is designed for easy access in lamp replacement. Minimum adjustment is required for the
replacement. The tungsten halogen lamp is seated in a specially designed holder that is located in the right
half part in the lamp compartment (Figure 11.1). However the deuterium lamp that comes up with a
ring-typed aluminum seat is located in the left half part respectively.

screw 1 screw 2 screw a screw b

Figure 11.1 Lamp compartment

CAUTION: Switch off and disconnect the instrument from the mains supply and allow the lamp to cool
down at least 15 minutes before proceeding.

WARNING: Do not touch lamps with bare hands. Use glove or cloth to prevent staining of the bulb.

WARNING: Pay extreme attention not to touch parts other than those as instructed.

Replacement of tungsten halogen lamp:

♦ Remove the cover of lamp compartment.
♦ Unscrew the retaining screws (screws a and b in Figure 11.1) of the holder of tungsten halogen lamp.
And pull the holder straightly upward.
♦ Pull out the damaged lamp from the socket to remove.
♦ Use lens paper to hold the new lamp for replacement.
♦ Put the whole set back to the original position and screw it tightly
♦ Remount the cover of lamp compartment.

Replacement of deuterium lamp:

♦ Remove the cover of lamp compartment.
♦ Find the rapid connector with red, black, and blue wires and disconnect it.
♦ Unscrew the retaining screws (screws 1 and 2 in Figure 11.1) on the white aluminum seat for the
49
 Metertech Inc.

deuterium lamp. Pull the deuterium lamp straightly upward to remove.

♦ Put a new deuterium lamp back to the holder and screw it tightly.
♦ Reconnect the rapid connector of the deuterium lamp.
♦ Remount the cover of lamp compartment.

WARNING: For replacing deuterium lamp or halogen lamp, be sure to contact your local agent, and use
vendor’s designated light sources.

Light source specifications:

Halogen lamp: 12V, 20W, and lifetime 2000 hrs.
Deuterium lamp: ignition voltage 500V, operating voltage 75-95V, operating current 300mA,
and lifetime 500 hrs.

50
 Metertech Inc.

Appendix A THERMAL CONTROLLER OPERATION

§A-1. Installation of thermal controller on SP8001 machine

-Power off SP8001 machine.

-The thermal controller can be applied to SP8001 in two approaches. It can be an
independent unit for rectangular cuvette, or accompanied with sipper for flow
through cell.
-For a standard alone thermal controller, a 24 pin to 9 pin cable adapter is required.
For operating with sipper, please refer to the operation instruction of sipper.
-Power off SP8001 machine.
-Plug the cable adapter into receptacle at the bottom right wall of SP8001 cell
compartment. Remove single cell holder by loosing three M3 screws, locate the
cell holder of thermal controller at the same position as single cell holder did.
Then tighten it with three M3 screws.
-Connect the 9-pin connector of thermal controller with that of cable adaptor.( if
sipper is required, connect 9-pin connector of thermal controller to that of sipper).

§A-2. Operation of thermal controller

-Power on SP8001 machine. For a proper thermal controller installation, a message

“Detect Accessory…..Single cell+TC(or Sipper+TC)” under the company logo
window of LCD display will be shown. If it displays “Detect Accessory….Single
Cell”, please power off machine and check thermal controller installation until
“TC” message is appeared.
-The default temperature setting is 25℃ when the machine is started. If you can not
get the correct reading at the bottom-right corner of LCD after 5 minutes from the
machine was powered, please turn off machine and inspect the cause of problem.
-Select #6 under System Main Menu of LCD display, and go to the System setup
menu. Then pick #5 and go to Accessory parameter setup. Using up/down arrow
key to select #4 (setup temperature), here you can choose one temp setting from
37℃, 30℃, 25℃ and none(no heating or cooling action) by left and right arrow
key. Finally press ENTER key, the temp controller starts to climb up or fall down
to your last temp setting. The settling time from one temp to another is less
than 5 minutes, and temp will be stabilized within +/- 0.1℃.
-Return to system main menu, pick #4 (kinetic mode). Pick #10(measure temp) of
the parameter setup, you can also select the temp setting for your experiment.

51
 Metertech Inc.

§A-3. Important notes

-The thermal controller is designed to adapt for two types of flow cell; aperture
height with 8.5 mm and 15 mm. The default height is 8.5 mm. If 15 mm height is
needed, please remove an aluminum block right down the bottom surface of cell
holder with a cross type screw driver.
-If you are using flow cell for experiment, and want get the best light transmission
through cell aperture. Please fill pure water into cell and make sure no air bubble
inside the aperture. At the Lambda mode of SP8001, key in 500nm, remove cell
from cell holder, and AUTOZERO to get 100%T. Put in flow cell with water,
loose three screws of thermal controller, and move it forward/backward with
respect to front panel until getting reading greater than 50 %T.

52
 Metertech Inc.

Appendix B SIPPER OPERATION

§B-1. Installation of sipper on SP8001 machine

-Power off SP8001 machine.

-Remove the front panel of cell compartment of SP8001 by loosing two M3 screws
behind it.
-A sipper assembly as shown on fig. 1 includes one pumping drive, one pinch
valve(one sample input, and another air input) two connectors( small one for
connecting to electronically thermal controlled cell assembly, large one for
connecting to SP8001 machine), two flexible tubes, and front panel. The latter one
contains sample solution inlet and outlet; thermal controlled water circulation inlet
and outlet, and one button.
-Locate the sipper assembly above the cell compartment, plug its large connector
into receptacle on the bottom-right wall of cell compartment, set connector cable
under sipper assembly.
-Move down the sipper until touching bottom surface, then tighten the sipper on the
bottom surface with one M3 screw located at opposite side of sipper’s front panel,
and one M4 screw beside the pumping wheel.
-Connect two flexible tubes of sipper to those of flow through cell(make sure the
length of cell’s both tubes are cut properly with length 55 mm).

§B-2. Operation of sipper

-Power on SP8001 machine. For a proper sipper installation, a message “Detect

Accessory…..Sipper” under the company logo window of LCD display will be
shown. If it displays “Detect Accessory….Single Cell”, please power off machine
and check sipper installation until “Sipper” message is appeared.
-Press F4 (Sample Control) under System Main Menu of LCD display, and you are
going to the sipper parameter setup mode. For a first time using sipper, there is a
set of default parameters shown on the LCD, these numbers are suitable for a 10
mm-long flow through cell with aperture diameter 2mm and aperture height 8.5
mm. It gives minimum sipped sample volume around 700 ul with reasonable
carryover(depends on the sample viscosity).
-Key F1(wash cell) is for continuously cleaning the cell and tube for 20 seconds.
F2(sipper test) is to test the sipper operation with latest parameters setting. F3 for
printing out the whole parameter setup. F4 for retrieving default parameter saved
in ROM. F5& Esc for going back to last screen. The keyed in parameters will be
saved in memory.

53
 Metertech Inc.

-There are six parameters to be set for the sipper to be properly operated. #1 is
pump speed selection for sampling solution at low , medium or high speed. #2 and
#4 are for air purge times setting, the pump is always preset at high speed. If you
do not need air purge between two samplings, just key in zero value( it is
suggested to have air purge on during operation to remove the possible
accumulated air bubble inside the cell). #3 and #5 is for sample sipping time
setting. These two numbers added together gives the total sampling time for a
complete sipping operation. # 6 is the delay time to read the sample after the
sipping action is stopped.
-In normal operation of the sipper, a button on the sipper front panel has the same
function as key “READ”. When in the sipper parameter setup mode, key “READ”
, “AUTOZERO”, and front panel button are not active. They function after
leaving this mode.
-Sometimes you do not want do measurement with sipper operated, you can pick #6
(system setup) under system main menu. Then pick #5(setup hardware), then pick
#2(switch to single cell). The machine will operate like only one cell installed
without actually removing sipper unit. You can also switch it back to sipper mode
by selecting “NO”.

§B-3. Important notes

-At the first time running sipper, checking the centering of flexible tube sitting on
white roller of pumping wheel is very important. Please enter F1 under sipper
setup mode to start the pump. If the tube gradually moves towards one side of
white roller while wheel is rotating, turn the stop (fig.1) 45/90 degrees cw/cww
until the tube returning to center position.
-The peristaltic tube at abnormal condition may not be tightened enough across
pumping wheel, such that the sipping force is too small to displace proper amount
of solution into the cell. Please check the tube fitness into the stop, or cut the tube
shorter(1mm off at one time) to generate more elastic force.
-If flow through cell is used for measurement, cell alignment for max light to pass through
aperture is required. Fill the flow cell with water either by sipping or other method, then go to
Lambda mode and set wavelength 500 nm, remove flow cell from cell holder, enter
“AUTOZERO” and obtain 100%T. Put cell back to cell holder and cover the cell compartment,
a good reading would be larger than 50%T(for aperture diameter 2 mm). If reading is smaller
than 40 %T, please loose three M3 screws around the cell holder, and move it
forward/backward with respect to front panel(there is only 2 mm max moveable displacement)
until finding the max reading. Finally tighten these screws.

54
 Metertech Inc.

flow cell& tubes

peristaltic tube
pinch valve
button stop 9-pin(small) connector

screw hole
sample inlet 24-pin(large) connector
sample outlet
pumping wheel
thermal water inlet&outlet

Figure 1. Sipper Assembly(with flow cell connected)

55
 Metertech Inc.

Appendix C SETTING OF USB SERIAL PORT

If your Windows(XP/98SE) PC has USB ports only, please install the attached USB to RS232 driver on PC.
Connecting the USB to RS232 cable between PC and the machine, then power it up. Please set up your
Windows(XP) computer as follow.

Under ‘Systems Tasks’ of ‘My Computer’ screen, press ‘View system information’. In System
Properties, select icon ‘Hardware’ and press button ‘Device Manager’(figure C-1).

In Device Manager, double click ‘USB Serial Port’(figure C-2) to enter port setting(figure C-3). Please
remember the com-port number for further use. Fill in ‘57600,8,None,1,None’ in those blanks. Press
button ‘Advanced..’ to enter advanced COM setting(figure C-4). USB transfer size is to be selected as max
number(4096). Latency timer is to be smallest number(1)

(Figure C-1)

56
 Metertech Inc.

(Figure C-2)

(Figure C-3)

57
 Metertech Inc.

(Figure C-4)

58