BIOSTAT® B-DCU II

Advanced Additive Flow

Culture vessel
Jacketed culture vessel fully equipped with:
– Sensors for Temperature, DO, pH and Foam
– Stirrer shaft with single mechanical seal
BIOSTAT® Fermentors | Bioreactors

– 3-blade segment impeller

– Aeration tube with micro Sparger,
Overlay aeration fitting, sterile filters
and exhaust cooler
– Manual sampler with sampling pipe
– Removable addition bottle support
– Addition bottles with stainless steel head
piece and sterile filters
– Inoculation|addition septum port
– Multiple way addition port
– Tube, O-ring and tool kit

SCADA Software MFCS/DA

To accelerate your research activities, a
powerful supervisory software MFCS/DA
for extended visualization, data acquisition
and trend display is included.
The BIOSTAT® B-DCU II is the second genera- Supply Tower – Plug and Play configuration
tion of a well proven Fermentor |Bioreactor – Batch-oriented software package
system, designed for meeting demanding Advanced Additive Flow Gassing System – Online data acquisition
requirements in both research and process – Sparger and Overlay or optional individual – Sample Data Management
development. Unrivalled for scale-down gas out lets – Enhanced Plotting
and scale-up modelling of various culture – Gasmixing of Air, O2, N2, CO2 for – Export functions
processes, the BIOSTAT® B-DCU II provides Sparger gassing via solenoid valves – Easy to use programming interface
a new level of power and flexibility. – Air for Overlay gassing
The BIOSTAT® B-DCU II Advanced Additive – Additional optional gas flow path Features
Flow packages are specially configured for – Optional gas switch Sparger to Overlay – Powerful industry rated BioPAT® DCU control
cell culture. The integrated automatically- – Up to 6 optional mass flow controller system with 15” TFT color touch screen
controlled gas mixing system for up to 6 gas – Independent process control for up to
flow path provides Sparger and Overlay or Pumps six culture vessels
optional individual gas out lets. Each gas has – 3 integrated pumps, expandable up to 6 – UniVessel® from 0.5 L to 10 L working
its own rotameter for individual flow rate – Up to 4 feeding pumps volume
adjustment. – Up to six integrated peristaltic pumps with
Temperature System choices for fixed and analogue speed pumps
Control Tower – Powerful heater (1 kW) – Choice of polarographic or optical
– Graphical user interface with color display – Integrated controlled cooling water valve pO2-sensors
and touch screen operation – Circulation pump – Superior gas mixing with up to 6 Rotameter
– Measurement and control for Temperature, – Temperature range 8°C above cooling and Mass Flow Controller
pH, DO, agitation and Foam water up to 80°C – Culture vessel pressure control
– User configurable 5-stage DO cascade – Easy on-site Supply Tower|Culture vessel
– Up to 4 feed controller per vessel Agitation System upgrade
– Automatic gas composition controlled – Speed range 20 up to 2,000 rpm – Improved connectivity of utilities and
by pH & pO2 controller – Maintenance free probes
– Level control via Level probe balance – Gear-free for quiet operation – Easy upgrade of cell culture packages
– Totalizers with digital calibration for for multi-purpose use
valves and pumps – Inclusive Supervisory Process control
– In-process pH-recalibration software
– Trend display for up to 8 process values – Validation support available, inclusive
– Up to 4 balance connections per vessel Logbook and 3-Level password protection
– Culture vessel pressure measurement
and control
Technical Specification
Space requirements|Enviromental conditions:
Space requirement 1-fold|2-fold|3-fold|4-fold|5-fold|6-fold [W+H+D] (without options) 800|1200|1700|2050|2550|3000+780 (10L: 820) +800 [mm
Space requirement autoclave d H (with BB-8844593 flexible adaptor for 170+340 (N/A)|240+500 (340)|270 +550 (400)|300 +700 (510)|
exhaust cooler) 0.5 L|1 L|2 L|5 L|10 L without tray for storage bottles 350+820 (620) [mm]
Ambient temperature|relative humidity (non-condensating) 5–40°C|85%
Control Tower
Housing material Stainless steel AISI 304
Display Touch Screen 15"
Resolution 1024+768 dpi
Communication Control Tower/Supply Tower |Control Tower/Host Ethernet|Ethernet
Measurement ranges|resolution
Stirrer speed 0.5 L|1 L|2 L|5 L|10 L tbd|20–2000|20–2000|20–1500|20–800 [rpm]|1 rpm
Temperature 0–150°C|0.1°C
pH 2–12|0.01 pH
pO2 0–250%|1%
Foam and Level on/off|4 user selecatable sensitivities
Turbidity (option) 0-6 AU|0.01 AU
Redox (option) -2000–2000 mV|1 mV
Pressure (option) 0 - 1000 mbarg|1 mbar
Gassing System "Advanced Additive Flow" with up to 6 gas flow paths 4-gas mixing system with Spager and Overlay gas outlets
Outlet design Hose tube OD 6 mm
Flowmeter Air calibrated @ 1.21 bara 20°C
0.5 L 1L 2L 5L 10 L
Gas flow range "Sparger" for Air & N2 0.53-53 0.53-53 13-133 13-133 26-266 [mL/min]
Gas flow range "Sparger" for O2 & CO2 1.5-15 1.5-15 3.3-33 3.3-33 13-133 [mL/min]
Gas flow range "Overlay" for Air 0.11-1.05 0.11-1.05 0.16-1.6 |0.42-4.2 1.3-13 [L/min]
Additional gas flow path to "Sparge" or "Overlay" Various flow ranges available from 1.5–15 mL/min to 2.3–23 L/min
Accuracy +/– 2% FS
Thermal Mass Flow Controller (option) Calibrated to specific gas
0.5 L 1L 2L 5L 10 L
Gas flow range "Sparger" for Air & N2 0.5-50 0.5-50 3-150 3-150 6-300 [mL/min]
Gas flow range "Sparger" for O2 & CO2 0.15-15 0.15-15 0.6-30 0.6-30 3-150 [mL/min]
Gas flow range "Overlay" for Air 0.02-1.0| 0.02-1.0 0.03-1.5 0.1-5 0.2-10 [L/min]
Additional gas flow path to "Sparge" or "Overlay" Various flow ranges available from 1.5–15 mL/min to 0.4–20 L/min
Accuracy +/– 1% FS
Agitation Maintenance and gear-free servo drive
Motor performance torque 0.5 L|1 L, 2 L, 5 L, 10 L 75/0.28|200/1.03 [W]/[Nm]
Integrated pumps on|off, pulse-width modulated controlled
Pump head Watson Marlow 102R, for tubing with 1.6 mm wall thickness
Rotation speed speed 20 rpm
Flow rate tube dependent (bore+wall tubing) 1.6+1.6/3.2 +1.6 [mm] 0.4-4.4|1.6-16.2 [ml/min]
Integrated feed pump (option) Speed-controlled
Pump head Watson Marlow 102R, for tubing with 1.6 mm wall thickness
Rotation speed speed 1-10|5-50 [rpm]
Flow rate tube dependent (bore+wall tubing) 1.6+1.6/3.2 +1.6 [mm] 0.2–2/0.8-8|1.1–11/4–40 [ml/min]
Temperature control system Thermostat system with recirculation pump and soleniod valve for cooling water
Temperature control range 8°C above cooling water to 80°C
Electrical heater 1000 W
Connections to culture vessel|Exhaust cooler Quick couplings|Quick couplings
External connections
Balance standard|option/interface/connector 2|4/RS232/M12
CO2 exhaust analyzer interface/connector 1/RS232/M12
Feed pump connection qty./interface/connector 2/0–10 V/M12
External inputs qty./interface/connector 2/0-10 V/M12
Culture vessel 0.5 L 1L 2L 5L 10 L
Design Jacketed glass vessel with stainless steel head pleate
Total volume 0.75 1.6 3 6.6 13 [L]
Working volume 0.15-0.5 L 0.4 -1 0.6-2 0.6-5 1.5-10 [L]
Headpleate ports 19 mm|12 mm|6 mm –|6|4 3|2|6 3|2|9 3|3|8 7|2|9
Volume storage bottles 250 250 250 500 500 [mL]
pO2 sensor|connector Polarographic or optical (option)|VP
pH sensor|connector Gel-filled|VP
Temperature sensor|connector Pt100 with pocket|M12
pH|Redox sensor (option) Gel-filled|VP
Turbidity sensor (option) Single Channel NIR Absorption Probe
Pressure transmitter (option) Piezoresistive|M12
Material (product wetted parts) Borosilicate glass|Stainless steel AISI 316L|EPDM
Utility consumption
Power consumption max. DCU Tower |Supply Tower 200|2000 [W]
Water consumtion max. per Power Supply 5 L/min
Gas consumption max. per Power Supply 0.5 L|1 L|2 L|5 L|10 L 1|1.6|4.2|13|20 [L/min]
Utility requirements |Housing connection
Power supply DCU Tower|Supply Tower 110 – 230V/6A GFIC: 32mA|120V/15A or 230V/10A GFIC: 32mA
Gasses Controlled @ 1.5 barg (22 psig); dry, particle and oil-free|hose connector OD 6 mm
Water Controlled @ 2–4 barg (29–58 psig)|hose connector OD 10 mm
Drain Gravity drain with zero backpressure required|hose connector OD 10 mm
Regulatory compliance CE (build according to UL & CSA requirements)
Ordering information
Description BIOSTAT® B-DCU II
Advanced Additive Flow
Culture vessel working volume 0.5 L 1L 2L 5L 10 L
Cat. No. 120 VAC
BIOSTAT® B-DCU II Single RBD1C5DLATSDG1 RBD1C01LATSDG1 RBD1C02LATSDG1 RBD1C05LATSDG1 RBD1C1ALATSDG1
BIOSTAT® B-DCU II Upgrade Kit
(Vessel + Supply Tower) RBD1C5DLATSDG1E RBD1C01LATSDG1E RBD1C02LATSDG1E RBD1C05LATSDG1E RBD1C1ALATSDG1E
Cat. No. 230 VAC
BIOSTAT® B-DCU II Single RBD1C5DLATSDG2 RBD1C01LATSDG2 RBD1C02LATSDG2 RBD1C05LATSDG2 RBD1C1ALATSDG2
BIOSTAT® B-DCU II Upgrade Kit
(Vessel + Supply Tower) RBD1C5DLATSDG2E RBD1C01LATSDG2E RBD1C02LATSDG2E RBD1C05LATSDG2E RBD1C1ALOTSDG2E

Control Unit
Control Tower
15’’ color display with touch screen operation •
Automatic pH and pO2 calibration routine
Single probe and group calibration •
Control Capabilities per Vessel
Temperature, pH, DO (5-stage cascade),
Stirrer speed, Foam, Substrate •
Vessel Pressure measurement |control • |••
Turbidity measurement •
Gravimetric Feed Control •
Gravimetric Harvest Control •
Extended Password Module •
Logbook Module •
Supply Tower
Gas mixing (integrated) Advanced Additive Flow
Gas outlets
Sparger & Overlay|Individual •|••
Rotameter Sparger • Air, O2, N2, CO2
Rotameter Overlay|Additional gas • Air |•• O2 or CO2 or N2
Solenoid valves for gas mixing of Air, O2, N2, CO2 •
Gas switch Sparger to Overlay •
Mass Flow Controller • (up to 6)
Stirrer Motor •
Peristaltic Pumps (integrated) 3
Feed Pumps speed controlled (integrated |external) • (up to 3)|•• (up to 2)
Thermostat system (integrated)
Jacketed vessel|Single wall vessel •|••
Supervisory Process Control Software
MFCS/DA for data storage •
Culture Vessel UniVessel®
Jacketed vessel|Single wall vessel •|– •|•• •|•• •|•• •|••
Stirrer shaft with Single Mechanical Seal
Direct coupling|Magnetic coupling •|– •|•• •|•• •|•• •|••
Magnetic drive – – • • •
3-blade segment impeller 1
Storage bottles 3 3 3 3 3
Air Inlet and Exhaust filter 3
Aeration tube with μ-Sparger |Overlay port •
Inoculation|Addition port •
Exhaust Cooler •
Addition fitting 2+3-way 4-way 4-way 4-way 4-way
Sample-|Harvest pipe •
Manual sampler •
Tray for storage bottles – • • • •
pH Electrode, cable •
DO Electrode , cable, Clark principle|Optical • |•
Foam sensor, cable •
Level sensor, cable •
Temperature sensor Pt 100 •
Turbidity sensor •
Exhaust CO2 sensor •
Pressure sensor| control • |••
Tubing, O-Ring (spare set) •
Broad range of accessories available; Configurable and customizable solutions are available outside of this package. Please contact us for further information.

• = standard, • = option, – = not available

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Publication No.: SBI2028-e09061 · Order No.: 85034-537-80 · Ver. 06 | 2009

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