2L glass standard fermentor (Off-site sterilization by autoclave, top mechanical drive) KNIK-2GJ

QUOTATION

TTL
UNIT
SEQ. ITEM DESCRIPTION QTY SUM
PRICE(USD$)
(USD$)
10L glass standard fermentor
1
KNIK- 10GJ (Off-site sterilization by 6 16,000 96,000
autoclave)
Remarks:
1. The price is CIF Manzanillo Mexico(Sea freight).
2. The price includes installation, debugging and training charge for customer.
3. Delivery time: It will take 45-60 working days after signing a contract and receiving
deposit.
4. Payment item: 50%TT as deposit, 50%TT after FAT and before shipment.
5. Guarantee: 1 year. We will solve the quality problem by the ways as internet training,
changing parts and engineer arriving customer site for repairing for free.
 The below typical combination 4 unit parallel 2L glass fermentor system with
one control cabinet is only for your reference.

Part 1:10L glass standard fermentor (Off-site sterilization by autoclave)

No Item Description
1 Vessel system Total volume: 10L, Working volume: 40-70%

Stainless steel cylinder combines with glass partly. It uses a large

area of highly refractory glass to observe material easily. Magnetic
force stirrer without axes sealing. There is no possibility to leak
out the bacteria. The view is spacious. The agitation system is
hanged up. There is no thimble at the bottom.
Ratio of diameter to high: 1:2
2 Sterilization method Off-site sterilization by autoclave
3 Aeration system Include rotameter, aeration filter
4 Agitation system GJ：Mechanical sealing system at the top of the fermenter;
high-powered stirring oar, antifoam oar; AC electrical machine:
infinite speed variation; rpm in the 1L germ fermenter: 70~1000rpm±1%.
The height of the stirring oar can be adjusted, too.
5 Temperature Automatically control .
control Interlayer electric calefaction
6 Airflow control Manual control, Flow meter display, aeration with 1 gas
7 Antifoam control Automatically check foam, automatically add antifoam by peristaltic
pump.
8 Feeding control Control by peristaltic pump switch, automatically feeding and
computation.
9 pH control Automatically add acid, alkali by peristaltic pump and control pH
accurately.
10 DO control Automatically control with rotating speed relatively.
11 Pressure control Manual control
12 KNIK-B Biology Control parameter: temp, pH, DO, antifoam, stirrer rev, feeding, liquid
process controller level
13 KNIK B Control System: on-site controller, the next crew for the
Siemens S7-200 series PLC control system, it is a mature, stable,
universal type of PLC, to suit a variety of automation applications,
especially in manufacturing process control Application. Its modular,
easy to implement distributed configuration and easy to master and so
on, Siemens PLC control center has become a biological reaction
process control implementation is economical and advanced control
system, the system uses the touch screen display, on-site direct
operations (menu type), all English menu and interface; also with the
host computer for data transmission, data sampling and display tank,
the system view of data analysis, reading settings.
Control system 10-inch LCD touch-sensitive screen as the display screen to display
complete content-rich, user-friendly screen is simple, easy to operate.
And disturbing each other. The control system can adapt to different
range of sensors and actuators the output of the input signal with the
signal anti-jamming system.
Software: Siemens S7+ FORCE CONTORL
Data collection and control module: Siemens S200 PLC controller and
data collection and control module
1. Control
① Manual control: You can set the percentage of open valve or a valve
opening ② automatic control mode: You can select continuous or PID
PID control the switch ③ sequence control: the control of all
 parameters can be pre-set at least 10 control section, ④ for automatic
segmentation remotely control: PC control ⑤ acceptable correlation
control: the dissolved oxygen can select speed, air flow rate, tank
pressure, control of feeding, etc.; pH can choose to control with acid and
alkali

2. Measurement function: can feeding, bubble enemy, such as

measurement of pH
3. XY-axis to chart real progress can be any expansion and contraction
of graphics display, to help the operator determine the parameters of the
interaction between the effects of the fermentation process to quickly
modify and adjust
4. Data Processing
1) to show that all the parameters of the trend curve analysis
2) to store, display, analyze all the historical curve parameters
3) can display all the parameters of the batch report
4) copy the data directly with the USB

5) data storage format is compatible with EXCEL, EXCEL platform can

be to deal with
5. Controller self-protection function: to set the password, others can not
modify the fermentation parameters; and the controller is not lost due to
power settings for each parameter
6. Can the process control parameters, each parameter can have 10
block can be set
7. Time display of each parameter recording interval can be adjusted
8. Two parameters can be carried out with the screen settings and
calibration
9. show the controller is really running against, the panel instructed the
state process system, process hardware, and motion status indicator for
understanding the various devices (such as peristaltic pumps,
circulating pumps, heating, mixing, etc.) work status, Fault identification
can be used as display
10. Each parameter of the transmission are independent modules,
without disturbing each other, for easy maintenance
11.PH, DO and other parameters of control and associated controls can
be adjusted

Part 2: Technical parameter:

Seq. Parameter Guideline Controller data processing
function
1 PH Displaying range: 0.00~14.00±0.01 pH value historical and trend
Auto-control range:2.00~12.00±0.05 curve analysis
 Fermentation process control Acid, alkali dosage curve analysis
(According to the fermentation time, auto- Batch report form analysis
control pH fluent, at least10 parts) Acid, alkali total dosage record
Keep and deliver data
2 DO 0-150±3％，Displaying precision: 0.1％ DO value historical and trend
It can be controlled by rev and feeding. curve analysis
Fermentation process control Batch report form analysis
(According to the fermentation time, auto- Keep and deliver data
control DO fluent, at least10 parts)
3 Antifoam Antifoam value historical and
Automatic PID control and alarm trend curve analysis
Fermentation process control Foam error state record
(According to the fermentation time, auto- antifoam total dosage record
control antifoam fluent, at least10 parts) Batch report form analysis
Keep and deliver data

4 Automatic enactment and control Rev value historical and trend

Rev （50~1200rpm±5‰） curve analysis
Fermentation process control Batch report form analysis
(According to the fermentation time, auto- Keep and deliver data
control rev fluent, at least10 parts)
5 Temperature of cooling water: +5℃-60℃ Temperature value historical and
Temperatu ±0.1℃ trend curve analysis
re Fermentation process control Batch report form analysis
(According to the fermentation time, auto- Keep and deliver data
control temperature fluent, at least10 parts)

6 PID automatic enactment and control Feeding value historical and trend
Feeding Fermentation process control curve analysis
(According to the fermentation time, auto- Feeding total dosage record
control feeding fluent, at least10 parts) Batch report form analysis
Keep and deliver data
7 Pressure Manual control, instrument display

8 Air flux Manual control, instrument display

Part 3: System main parts

Seq. Product Description Quant. Source
GJ: stainless steel cover, Borosilicate glass
tank, stainless steel round jacketed bottom and
1 Tank 5mm stainless steel jacketed tank, control 1 KNIK
temperature together, heating by bottom
heating blanket.
 United
2 Machinery seal John Crane 1
Kingdom
3 Stirring motor Mechanical stirring: TECO 1 TECO
4 Speed governor Mechanical stirring: TECO 1 TECO
5 Silicon pipe Lange 10M Baoding
Germany Siemens S7-200 series PLC control
system +10 inch Kunlun-type LCD touch
screen + circuit control board + electrical boxes
6 PLC system and various types of electrical appliances. 1 KNIK
Including cabinets, control core, signal
acquisition, transmitter, power drive and other
types of actuators
7 Rotor flowmeter LZB-10, 10-600L/H 1 China
8 Air intake filter Sartorius, 0.2μm φ50 1 Sartorius
9 Breathing filter Sartorius φ37 4 Sartorius
Feeding peristaltic
10 matched 1 China
pump
Peristaltic
11 matched 1 China
pump(acid)
Peristaltic
12 matched 1 China
pump(Alkali)
Peristaltic
13 matched 1 China
pump(Defoaming)
14 PH electrode Hamilton or Mettler 1 Switzerland

15 PH cable Hamilton or Mettler 1 Switzerland

16 DO electrode Hamilton or Mettler 1 Switzerland

17 DO cable Hamilton or Mettler 1 Switzerland

Temperature
18 PT100 1 China
electrode
Electromagnetic
19 DN8-UNIT 1 China
valve
Feeding bottle, silicone tube, pipe, pipe rack,
20 Others all kinds of valves, heaters, pressure gauges, 1 Shanghai
bearings, seals, tools, etc.

Part 5: Spare parts list

Seq. Product Specification Quantity Original

1 Breathing filter 0.2μm，φ37 6 pcs Germany

2 Air intake filter 0.2μm φ50 2 pcs Germany

Shanghai
3 Feeding bottle 500ml 2 pcs
Shanghai
4 Feeding bottle 1000ml 2 pcs

Feeding bottle two-way

5 standard 4 pcs Shanghai KNIK
interface
standard
6 PH Standard solution 1 set Shanghai
standard Shanghai KNIK
7 Single hole feeding pin 4 set
standard Shanghai KNIK
8 Silicone plug 30 pcs
Silicone tube
9 16# 5m China
Silicone tube
10 25# 5m China

11 O ring standard 20 pcs Germany

Shanghai
12 Fuse 5A/10A 15 pcs
Shanghai
13 Tool standard 1 set

Equipment operating
14 1 set Shanghai KNIK
instructions

15 Qualification Certificate 1 set Shanghai KNIK

16 Receiving report 1 set Shanghai KNIK

⚫ Working environment:
⚫ Up to the internal &external environment required by fermentation
⚫ Up to the technic-required water supply. The temperature of cooling water is at
least 5℃ below the temperature of fermentation
⚫ Power requirement: AC220/ 400W
⚫ Compressed air: Primarily-processed compressed air, relative
humidity:≤60%,ventilatory capacity is accorded to the technics.