ميحرلا نمحرلا هللا بسم
Omdurman Islamic University جامعة ام درمان االسالمية
Faculty of Pharmacy كلية الصيدلة
Department of Pharmacology قسم علم األدوية
E-mail: [email protected]
Tel/Fax: +249-187-511573, P.O. Box 382
الفرقة الرابعة
)5 (عملي علم األدوية رقم
Behavioral Pharmacological experiments(3)
Assessment of local anesthetic activity
Introduction:
LAs are group of drugs that affect the CNS by blocking the action
potential generation and sometimespropagation by blocking Na+
channel. They are amphiphilic molecules with hydrophobic aromatic
group, and basic amine group.
Objectives:
To be able to carry out the preparation of spinal frog.
To compare practically between LAs in onset time.
To develop the ability to analyze, summarize and represent
scientific data.
Materials and Methods:
Animals:Wister albino SWR rats, weighing (70 – 200)g.
Chemicals:
Lidocaine 30mg/10ml, and 60mg/10ml
Procaine 10mg/10ml, and 50mg/10ml
Diluted HCl 0.05 M, 0.1 M, and 0.2 M
Normal saline (0.9%)
Equipment:
Cork board, forceps, dissection set, beaker, syringes and pin.
Experimental protocol:
Preparation of spinal frog: Stunning and decapitate the frog.
Preparation of sac: Remove the exposed viscera from the
abdominal pouch with fine forceps (take care not to damage the
nerves at the base of the spinal cord).
Mounting: Pin the frog by its front limbs to a vertically mounted
cork board so that the hind legs are suspended freely below it.
N.B: It is important to begin the experiment immediately.
Procedure:
1- Dip both hind legs into a beaker containing 0.05 M HCl and note
the time taken for the frog to withdraw its foot. If no response is
observed after 10 seconds, try 0.1 M HCl and 0.2 M respectively.
If this is ineffective exclude the frog from the experiment.
2- Immediately following a response (i.e. foot withdrawal) wash the
legs by immersing them in a beaker containing 0.9 % normal
saline.
3- Administer 1 ml of the local anesthetic to be tested into the
abdominal pouch and start the stop clock.
4- Test at 1 minute intervals the foot withdrawal reflex using the
lowest conc. of HCl that elicits a response. If a response is not
observed increase the HCl conc. (0.05 M to 0.1 M to 0.2 M). The
end point (i.e. a full local anesthetic effect) is taken as failure to
remove the feet from 0.2 M HCl after 10 seconds contact.
5- Repeat 3 and 4 for at least two concentrations of procaine and
lignocaine using a new frog for each determination.
Results:
Display your result as follow:
Analysis of data using descriptive type of statistical analysis
(Mean, SD and SEM)
References:
Range and Dale’s Pharmacology
Experimental Pharmacology and Toxicology.
Basic and Clinical Pharmacology-Katzung.SS
