Genomix Bovine Pregnancy • Use suitable protective clothing (gloves, lab coat, safety glasses)

when handling samples. Avoid any contact between hands, eyes,

RAPID TEST KIT nose or mouth during specimen collection and testing. Do not
pipette any material by mouth.
Please read instructions before use
• Do not smoke, eat or drink in areas where specimens or kit
materials are kept.
INTENDED USE • After the completion of the assay, autoclave all materials for 1
hour at 121ºC. Alternatively, materials can be treated with 10%
solution of bleach.
The Genomix Bovine Pregnancy Rapid Test is a lateral flow • Dispose of materials carefully in biohazard bags.
Immuno chromatographic assay for Bovine pregnancy. Early diagnosis
of pregnancy is of great importance in modern animal industry due to STORAGE AND STABILITY
the high cost of feed and labor. Especially after artificial insemination
is widely used, the earlier the pregnancy is determined, the earlier • Test device in the sealed pouch can be stored at 2-26ºC up to the
feeding plans will be performed. The general methodology to expiration date.
determine bovine pregnancy is by observation or ultrasound • Do not freeze the test device.
scanners, either of which is not quite economic in small or middle • The test device should be kept away from direct sunlight, moisture
size farms. The current bovine pregnancy rapid test kit is a 15min and heat.
lateral flow immunoassay kit that utilizes the high affinity of
monoclonal antibody against bovine pregnancy associated
SPECIMEN COLLECTION AND PREPARATION
glycoprotein (PAG), which can easily identify the concentration and
presence of PAGs.
• This test can be performed using serum or plasma. It is
DO NOT INTERPRET RESULTS
recommended that fresh samples to be used if possible. AFTER 20 MINUTES.
SUMMARY AND PRINCIPLE OF THE ASSAY
• Collect the whole blood by using the suitable anti-coagulant.
• Do not use the hemolysis blood. For serum sample, collect blood in
The Genomix Bovine Pregnancy Rapid Test is a lateral flow INTERPRETATION OF THE RESULTS
a tube without anticoagulant and allow clotting. For plasma
Immuno chromatographic assay. The test cassette consists of: a
samples, collect the blood in a tube containing anticoagulant.
nitrocellulose membrane strip containing a test band (T band) and a
control band (C band). When an adequate volume of test specimen is • Separate serum or plasma from blood as soon as possible to avoid
dispensed into the sample well of the test Cassette along with the hemolysis. Use only clear, non-hemolyzed specimens. *
Caution: Please ignore the test results after 20
buffer, the specimen migrates by capillary action across the cassette. • Testing should be performed immediately after the specimens have minutes.
The “Control Lines” is used for procedural control. Control line should been collected. Do not leave the specimens at room temperature Positive:
always appear if this procedure is performed properly and the test for prolonged periods. Distinct pink color bands appear at the control and test line regions.
reagents of control line works. A purple “Test Line” will be visible in • If the specimen cannot be tested on the day of collection, store the Negative:
the result window if there are enough Bovine Pregnancy specific specimen in a refrigerator at 2 to 4˚C for up to 3 days. If testing Only one pink color band appears on the control region. There is no
protein in the specimen. The specially selected antibody is used in test cannot be done within 3 days, specimens should be stored in a apparent color band on the test region.
line. Genomix Bovine Pregnancy Rapid Test Kit to identify Bovine freezer (-20˚C or colder). Invalid:
Pregnancy Antibodies in specimens. • Make sure to stir and bring the specimen to room temperature No visible band at control region. Repeat with a new test device. If
before testing. Do not freeze and thaw the specimen repeatedly. test still fails, please contact the distributor with lot number.
PACKAGE CONTENT • Specimens containing precipitate may yield inconsistent test results
such specimens must be clarified before assaying.
LIMITATIONS
The Genomix Bovine Pregnancy Rapid Detection Test Kit
contains the following items to perform the assay: TEST PROCEDURES
The Genomix Bovine Pregnancy Rapid Detection Test Kit will
• Pouch content: Test Cassette, Dropper, Desiccant.
only indicate the antibody presence against Bovine Pregnancy species
• Sample Diluent 1. Remove the testing device from the foil pouch by tearing at the
in the specimens. As with all diagnostic tests, all results must be
• Test instruction “notch”. Then place the testing device on a level surface.
interpreted together with other clinical information available to the
2. Holding the Sample Dropper vertically, add 60µl of specimen veterinarian. If the test result is negative and clinical symptom is
MATERIAL REQUIRED BUT NOT PROVIDED without air bubbles into the sample well and add one drop of persisting, additional testing using other clinical methods is
Sample Diluent marked with an arrow on the testing device. recommended.
• Clean, dry specimen collection container (plastic or glass). 3. Wait for the Purple colored test band to appear then read the
• Clock or timer. results. Positive results can be read as soon as it appears.
Negative results may be confirmed in 15 minutes. Ensure that the
PRECAUTIONS background of the test window is white before interpreting the
results.
• For in vitro diagnostic use only.
• Do not reuse.
• Test device should remain sealed until use.
• Do not use after the expiration date printed on the pouch.
• Keep out of children’s reach.