 PHAMACEUTICAL BIOTECHNOLOGY

RGUHS PREVIOUS YEAR QSNs (Dec-2020 to Jun-2024)

UNIT-I
10 MARKS QUESTIONS
1 Describe any three methods of enzyme immobilization. Illustrate the applications of immobilised JUN-2024
enzymes in medicine and production of pharmaceuticals.
2 Explain different methods of enzyme immobilisation with their advantages and disadvantages. Nov-2023
3 Explain different methods of enzyme immobilisation with their advantages and disadvantages Jun-2023
4 What are biosensors? Explain the types with pharmaceutical applications. Nov-2022
5 Explain different types of biosensors and their applications in pharmacy and medicine. July-2022
6 What is enzyme immobilization? Explain covalent bonding and gel entrapment methods with Nov-2021
advantages and disadvantages.
7 Write in detail the different methods of enzyme immobilization. Write its applications. Mar-2021
8 What are biosensors? Write its principle and its functions. Dec-2020

5 MARKS QUESTIONS
1 Describe the production and uses of penicillinase. List out advantages of production of enzymes JUN-2024
from microbial sources.
2 Describe the production and uses of lipase. NOV-2023
3 Describe the production and uses of lipase. JUN-2023
4 Describe the production and uses of amylase. Nov-2022
5 Describe the production and uses of catalase. Jun-2022
6 Describe the production and uses of amylase. Nov-2021
7 Explain the method for determination of immobilized amylase activity. Mar-2021

UNIT-II

10 MARKS QUESTIONS
1 Describe the production of insulin by recombinant DNA technology. JUN-2024
2 Describe the production of Hepatitis B vaccine. Write a note on plasmid vectors. Nov-2023
3 Describe the production of Hepatitis B vaccine. Write a note on plasmid vectors. JUN-2023
4 Write a detailed note on cloning vectors. Nov-2022
5 Describe the production of insulin by r DNA technology. Jun-2022
6 Describe the general method of recombinant DNA technology. Nov-2021
7 Write in detail three different vectors used in genetic engineering. Mar-2021
8 Write in detail the production of Insulin by genetic engineering method. Dec-2020

5 MARKS QUESTIONS
1 Explain the production of hepatitis B vaccine by rDNA technology. JUN-2024
2 What are restriction enzymes? Explain the types and their role in genetic engineering. JUN-2024
3 What are vaccines? Classify the types with examples. JUN-2024
4 Explain Polymerase chain reaction with applications. Jun-2023
5 Outline the production of interferons by rDNA technology. Jun-2023

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6 Explain the production of interferons by rDNA technology. Nov-2022
7 List out the applications of genetic engineering in medicine. Nov-2022
8 What are cloning vectors? Explain the features of pBR322. Jun-2022
9 What are molecular scissors? Explain their role in recombinant DNA technology. Jun-2022
10 Explain the production of insulin by rDNA technology. Nov-2021
11 Describe the technique of polymerase chain reaction (PCR). Nov-2021
12 Write a note on applications of genetic engineering in medicine production. Dec-2020
13 What is PCR? Write the working principle of PCR. Dec-2020
14 What are vaccines? Classify the types with examples. Jun-2023

UNIT-III
5 MARKS QUESTIONS
1 Describe the production of monoclonal antibodies by hybridoma technology JUN-2024
2 Outline the general method for the preparation of bacterial toxoids. JUN-2024
3 Describe the applications and method for production of monoclonal antibodies. Nov-2023
4 Outline the general method for the preparation of antitoxin. Nov-2023
5 Describe the structure of Immunoglobulin Nov-2023
6 Describe the applications and method for production of monoclonal antibodies. Jun-2023
7 Outline the general method for the preparation of live attenuated bacterial vaccines. Jun-2023
8 List out different blood products and their applications. Nov-2022
9 Outline the general method for the production of live viral vaccines. Nov-2022
10 Write the role of lymphocytes in immunity Nov-2022
11 Explain the production of antibodies from B lymphocytes. Jun-2022
12 Classify vaccines giving examples. Write the difference between toxins and toxoids. Jun-2022
13 What are monoclonal antibodies? Describe different methods of purification of MAb. Jun-2022
14 Classify immunity. Write the difference between active and passive immunity Nov-2021
15 Outline the general method for the preparation of live attenuated bacterial vaccines. Nov-2021
16 Describe the structure of an immunoglobulin with a neat labelled diagram. Nov-2021
17 Write a note on Immune stimulation and Immune suppression. Mar-2021
18 Write in brief structure and function of MHC. Mar-2021
19 Write a note on storage and stability of vaccines. Mar-2021
20 Explain the structure of immunoglobulins. Dec-2020
21 Write the production of killed bacterial vaccines. Dec-2020
22 Write the production of monoclonal antibodies. Dec-2020

UNIT-IV

5 MARKS QUESTIONS
1 Explain different types of mutations. Jun-2024
2 Describe the Southern blot test with its applications. Jun-2024
3 What is transduction? Explain the methods Jun-2024
4 What is biotransformation? List out the applications of microbial biotransformation. Nov-2023
5 Describe ELISA with its applications. Nov-2023
6 Explain different types of mutations. Nov-2023
7 What is biotransformation? List out the applications of microbial biotransformation. Jun-2023
8 Describe the Southern blot test with its applications. Jun-2023

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9 Explain transduction and conjugation. Jun-2023
10 Write a note on microbial biotransformation in production of steroidal medicinal agents. Nov-2022
11 Describe the Southern blot test. How does it differ from Western blot test? Nov-2022
12 Explain conjugation. Write its significance. Nov-2022
13 Describe ELISA with its applications. Jun-2022
14 Explain briefly, transformation, transduction and conjugation. Jun-2022
15 Explain different types of mutagenic agents. Jun-2022
16 Describe ELISA with its applications. Nov-2021
17 Explain microbial biotransformation with examples. Nov-2021
18 Write in brief the southern blot technique and mention its applications. Mar-2021
19 Write in brief about transduction. Explain the types. Mar-2021
20 Describe ELISA with its applications. Mar-2021
21 Write in brief the western blotting technique and mention its application. Dec-2020
22 Write in brief about transformation with suitable examples. Dec-2020
23 Write briefly about different types of mutation and give its significance. Dec-2020

UNIT-V
10 MARKS QUESTIONS
1 Describe the different methods of fermentation. Explain the production of griseofulvin by Jun-2024
fermentation technology with a neat labelled flow chart.
2 Describe the production of Penicillin by fermentation technology with a neat labelled flow chart. Nov-2023
3 Describe the production of Penicillin by fermentation technology with a neat labelled flow Jun-2023
chart.
4 Describe the production of citric acid by fermentation technology with a neat labelled flow Nov-2022
chart.
5 Describe the production of Penicillin by fermentation technology with a neat labelled flow chart. Jun-2022
6 Describe the construction and working of a fermenter with a neat labelled diagram Nov-2021
7 Write the design of an industrial fermenter with a neat labelled diagram. Mar-2021
8 Write in detail the different types of fermenters and its applications. Dec-2020

5 MARKS QUESTIONS
1 Explain the production of penicillin G by fermentation technology. Nov-2021
2 Define fermentation and write the production of Vitamin B12 Mar-2021
3 Write in brief the Collection, Processing and Storage of whole human blood. Dec-2020

* How to make sodium alginate beads in calcium chloride solution?

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