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Chapter

Natural Antioxidants: An Update

Muhammad Alamzeb, Behramand Khan, Ihsan Ullah,
Muhammad Omer and Adnan Rauf

Abstract

Antioxidants are the body's defensive mechanism against reactive oxygen species
damage, which is typically caused by the different physiological activities that take
place within the body. These antioxidants can be obtained from a variety of sources,
including the body's own endogenous antioxidants and exogenous dietary sources.
Generally, food items and several types of medicinal plants are considered as the
sources of natural antioxidants. Natural antioxidants possess wide variety of bioas-
say properties like anti-cancer, anti-aging, anti-inflammatory etc. The substitution
of artificial dietary antioxidants with natural ones in recent decades has increased
interest in low-cost raw materials, particularly agricultural-based products, for the
discovery of new antioxidants. For both natural and synthetic antioxidants, reports
of biological features such as anti-allergic, anti-mutation, anti-cancer and anti-aging
activity have been reported. The most significant natural antioxidants come from
regularly eating fruits and vegetables, although other plant materials and agricultural
waste are also major sources of antioxidants.

Keywords: antioxidants, vegetables, plants, fruits, herbs

1. Introduction

Reactive nitrogen and oxygen species (RNS and ROS), including nitric oxide
radicals, hydroxyl, and superoxide, can harm DNA in biological systems and cause
oxidation of proteins and lipids in cells [1]. Free radicals can typically be scavenged
by the body’s antioxidant system, which helps to keep oxidation and anti-oxidation
in the right proportion. But when the body produces too many ROS and RNS due
to exposure to toxins from the environment, radiation, alcohol, or cigarette smoke,
the body’s natural balance of oxidation and anti-oxidation is disturbed, which can
result in a number of chronic and degenerative illnesses [2, 3]. Intake of exogenous
antioxidants could be increased to minimize the consequences of oxidative stress by
scavenging free radicals, quenching singlet oxygen, and acting as reducing agents.
These antioxidants also operate as scavengers of free radicals and quenchers of singlet
oxygen [4].
Plants are the ultimate sources of natural antioxidants that are consumed or used
medicinally. Antioxidants are obtained from vegetables, mushrooms, fruits, spices,
cereals, flowers and herbs [5]. Additionally, antioxidants can also be obtained from
businesses that deal with agricultural byproducts [6]. Flavonoids, lignans, stilbenes,
anthocyanins and several other polyphenolic compounds, vitamins and carotenoids
1
Medicinal Plants - Chemical, Biochemical, and Pharmacological Approaches

like carotenes and xanthophylls are obtained and derived from plants [7]. The natural
antioxidants possess many pharmacological properties such as anti-cancer, anti-viral,
anti-inflammatory and anti-bacterial [2, 7, 8].
Antioxidants can be divided into two major classes Antioxidants can be divided
into two primary categories: Natural antioxidants and synthetic antioxidants. Free
radical damage predominantly affects the cellular level of the body, and antioxidants
protect against it there. As a result, enzymatic and nonenzymatic types of these anti-
oxidants are also possible. The three primary enzyme-based antioxidants are gluta-
thione peroxidase, catalase, and superoxide dismutase. The serum reflects the body’s
overall capacity for antioxidants, which is influenced by additional enzymes in the
body [9]. The non-enzymatic class of antioxidants can be classified in several classes.
Vitamins like vitamins C, E, A, peptides, enzyme co-factors (Q10) and a few minerals
(selenium and zinc) usually serve as building blocks of the natural antioxidants [10].
The classification of natural antioxidants has been shown in Figure 1.
Some of the unfavourable or detrimental effects of synthetic antioxidant use have
been uncovered by recent toxicological study. Researchers are now focusing their
efforts on locating natural sources with sufficient antioxidant activity as a result
of these studies. Furthermore, substantial concerns are raised regarding the cost
and availability of these natural antioxidants. It is possible to define the numerous
subcategories of natural antioxidants. Antioxidants, however, fall into two main
categories: those that are present in frequently utilized or regular food items (such as

Figure 1.
Classifications of Antioxidants obtained from natural sources [11].

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Natural Antioxidants: An Update
DOI: http://dx.doi.org/10.5772/intechopen.112462

beans, fruits, vegetables and cereals) and those that are present in plants or herbs that
have some antioxidant potential but are not frequently consumed (such as medicinal
plants and wild herbs) [12, 13]. Until now, researchers from all around the world have
concentrated on discovering inexpensive, more natural sources of antioxidants. These
findings will be utilised by the food, pharmaceutical, and beauty industries as an
improved option for produced supplements. Despite the fact that synthetic supple-
ments have not yet been proven to have substantial detrimental effects, supplement-
ing should generally emphasise getting back to nature. In the next ten years, natural
products-based items are going to get more and more important, and research in
the area of naturally occurring antioxidants will be more and more emphasized and
pursued [14, 15].
The current chapter’s major goal is to provide an overview and summary of the
natural sources having antioxidant potential.

2. Oxidative stress

Oxygen, which is essential for sustaining cell viability and metabolism and is
associated with aerobic living conditions, is also dangerous due to its paramagnetism.
The paramagnetic nature of oxygen results in creation of very reactive intermedi-
ates chemicals. These chemicals are referred to as “reactive oxygen species” (ROS).
These ROS are free radicals (FRs) in nature. Maximum natural stability refers to
the coupling of the electrons in the corresponding molecular orbitals of stable
neutral compounds. Because of this, when an orbital is having un-paired electrons,
extremely reactive chemical entities are formed. The chemicals have the natural
inclination to obtain an electron form neighboring molecules to account for their
electron deficiency [16]. The main free radical is the triplet state of oxygen which
possess two unpaired electrons. The rate of the reaction of triplet state is usually
slow, however, due to metabolic transformation into one or more very reactive
species it can dangerously interact with biological systems. This kind of metabolic
activation is generally preferred in biological systems due to conversion of O2 to H2O
during the phenomena of electron transport chain. During electron transport chain
ROS and FRs are produced due to transfer of electrons [17]. External stimuli like
sun radiation can induce free radicals to develop in biological systems since UV light
exists. UV rays causes the homolytic bonds between molecules to disintegrate. As a
disease worsens, FR can also manifest. For example, during heart attack, many FRs
are produced when supply of glucose and oxygen are interrupted to cardiac muscles.
Another outside factor which enhances the rate of formation of FR is known as
chemical intoxication. The organism promotes FR release because it needs to convert
toxic substances into less toxic ones. The toxicity of numerous numbers of drugs is
due to their inclination to produce FRs and interference with processes for the forma-
tion of FR. Similarly, food contamination with herbicides and chemicals may also act
a source of FR formation [18].
Inflammations are induced due to endogenous components which ultimately
results in the promotion of FR. The FR occur in immune system’s cleaning cells and are
responsible for removing dangerous microbes. Tissue damage comes from excessive FR
during this phase. Superoxide ions (O2•−) are generated NADPH oxidase in the phago-
cytic cells. O2•− which is thought of as the main ROS. It may produce secondary ROS
after interacting with other molecules through enzymatic processes. The protonation of
O2•− may result in the formation of H2O• and H2O2. When water is exposed to UV light,
3
Medicinal Plants - Chemical, Biochemical, and Pharmacological Approaches

molecular oxygen is exposed to cellular free radicals produced inside living cells, and
water is photolyzed, O2•− is produced. as demonstrated by hemoproteins, NAD•, FpH•,
semiquinone radicals, pyridinium cation radicals, etc. The phagocytic cells, during the
process of respiration and oxygen ingestion, also produce O2•− . The superoxide radical
does not undergo rapid reaction with nucleic acids, polypeptides or carbohydrates [18].
Cells create •NO as a defense mechanism when nitric oxide synthase interacts with
intracellular arginine. Lipid peroxidation in lipoproteins results from the creation
of ONOO, which is created when O2 and •NO mix. Autoimmune disorders which
clearly demonstrate this phenomenon include vitiligo, Graves’ disease, biliary cir-
rhosis, systemic lupus erythematosus, Hashimoto’s disease, Rheumatoid arthritis,
type 1 diabetes, inflammatory bowel syndrome, celiac disease, scleroderma, multiple
sclerosis and psoriasis.
These chemical species are necessary for many of the chemical reactions that
occur throughout metabolic activities, hence FR is required at all times. For instance,
FR plays a role in the polymerization of glucose and amino acids to make glycogen
and proteins.FR catalytically activates a variety of intermediary metabolic enzymes
such as lipoxygenase, cyclooxygenase, and monoamine oxidase etc [18]. Antioxidant
enzymes often successfully regulate these free radicals. Irreversible structural changes
in essential macromolecules such as lipids, DNA and proteins also act as sources of
ROS. Malonaldehyde and hydroperoxide, two substances that cause oxidative damage,
are produced by these mechanisms. Neutral species like N2O3 and ONOOH as well as
NO• , ONOOCO2− and NO2• ONOO− are all RNS. RNS are produced in small amounts
during cellular growth, production of cellular energy, signaling, blood pressure
modulation, relaxation of muscles, aggregation of platelets, neurotransmission and
phagocytosis etc. [19, 20].

3. Importance of antioxidants

Both biochemical and biological defense systems have been developed by

biological systems. in oxygenated circumstances. A microvascular system regulates
the tissues’ oxygen levels as far as physiological level is concerned, while at biochemi-
cal level, an enzymatic or non-enzymatic antioxidant defense system operates for the
repair of the molecules.

3.1 Primary enzymatic type systems

Aerobic species have produced antioxidant enzymes such catalase, glutathione per-
oxidase, superoxide dismutase, and DT-diaphorase. SOD is responsible for the dismu-
tation reaction that converts oxygen into hydrogen oxide, which is then converted back
into oxygen and water in subsequent reactions that are catalyzed by catalase or GPx.
The detoxification of a cell is performed by SOD. Because SOD requires a metal as a co-
factor to detoxify a cell. Depending on the kind of metal ion required by SOD as a co-
factor, different forms of the enzyme exist [21, 22]. CAT completes the detoxification
process that SOD began by catalyzing the reduction of H2O2. Iron or manganese act
as co-factor during the reduction of H2O2 and results in the production of water and
oxygen molecules [23]. CAT is so efficient that a very large number of H2O2 molecules
can be destroyed in a single second. Its main function is to eliminate the H2O2 created
when fatty acids are oxidised. Peroxisomes are where CAT is mainly found. A vital
intracellular enzyme called GPx breaks down lipid peroxides to corresponding alcohols
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Natural Antioxidants: An Update
DOI: http://dx.doi.org/10.5772/intechopen.112462

and H2O2 in water; this predominantly occurs in the mitochondria and occasionally in
the cytoplasm [24]. Selenium is necessary for the function of GPx. At least eight GPx
enzymes (GPx1 to GPx8) are found in human beings [25].
Almost all cells contain GPx1, the most common selenoperoxidase among gluta-
thione peroxidases. The enzyme is necessary to stop the oxidation of lipids, resulting
in protecting the cells from oxidative stress [26]. When GPx activity is low, the func-
tioning proteins and fatty acids in the cell membrane experience oxidative damage.
The production and prevention of GPx, particularly GPx1, have been associated to a
variety of diseases [27]. DT-diaphorase participates in the reduction of compounds
with a quinone structure and catalyzes the conversion of quinone to quinol. Cells
manufacture these enzymes under the direction of DNA [28].

3.2 Non-enzymatic type system

The antioxidants which capture FR, they constitute non-enzymatic type systems.
They catch FR in order to prevent the radical initiation reaction. However, they are not
as much reactive as that of the original FR. Antioxidants become free radicals in the
process of neutralizing or trapping the radicals by donating electrons. The FR from
antioxidants can be immediately and effectively neutralized by other antioxidants of
this family. The cells utilize antioxidants and FR like α-tocopherol (vit E), ferritin,
selenium, GSH, co-enzyme Q , zinc, bilirubin, cysteine, ascorbic acid (vit C), ubiqui-
none, melatonin and flavonoids. In some foods, the extracted flavonoids work with the
ROS directly to form non-reactive or less reactive complexes, but in other foods, the
flavonoids take part in the specific enzymatic catalysis as co-substrates [29].

4. Fruits and vegetables as sources of natural anti-oxidants

A class of chemicals with low and high molecular weights known as polyphenols is
found in fruits and vegetables and has the ability to inhibit lipid oxidation. In addition
to being functional derivatives like esters and methyl esters, most of them are the
conjugates either mono or polysaccharides with one or more phenol linkages. This
important class of natural antioxidants can be found in fruits like grapes, green and
red teas, and other teas, especially those that are caffeine-free [30].
Yet, the polyphenols in teas are more important than those in fruits because of
higher blood bioavailability. 15% to 20% of consumed polyphenols are absorbed by
the human circulation (Table 1). This absorption is enhanced when no sugar mol-
ecules are present. Teas absorb polyphenols at a rate that is higher than that of fruits
since fruits have a high sugar content [41, 42].
Flavonoids are also very rich sources of antioxidants. Food items like peaches,
potatoes, berries, wheat and almonds are the richest sources of flavonoids [43, 44].
A subgroup of flavonoids called anthocyanin is found in berries and red wine. It is a
potent antioxidant and has a lesser bioavailability than other flavonoids. Polyphenols
can display their antioxidant properties and prevent the growth of plaque through
low-density lipoprotein (LDL) oxidation [45]. Furthermore, it has been revealed that
particular types of polyphenols can stop some important enzymes from oxidising,
keeping their proper function. The family of carotenoids comes in second place to
polyphenols as a significant class of phytochemical antioxidants present in fruits and
vegetables. Veggies including potatoes, carrots, papayas, and apricots are the main
sources of them [11].
5
Medicinal Plants - Chemical, Biochemical, and Pharmacological Approaches

S. Common Antioxidant present ORAC value Reference

no. name (mmolTE/g)

1 Plum Flavonoids, Phenolic Acids, Proanthocyanidins, 94.8 [31]

Hydroxychalcones, isoprenoid glycosides.

2 Pomegranate Polyphenols and Vitamin C and 1250

3 Guava Carotenoids, Lycophene, Vit C, anthocynin [32]

4 Pears Vit C, betalains, tauline, total carotenoids, 140 [33]

flavonoids and total phenolics

5 Beet root Carotenoids, Flavonoids 4100 (dry [34]

Vit C and Vit E, extract)

6 Apple Flavonoids, Proanthocyanidins, , Phenolic 17.0 [31]

acids, Isoprenoid-glycosides, Flavanols,
Hydroxychalcones etc.

7 Papaya β-sitosterol, Quercetin 300 [35]

8 Pea Carotenoids, Flavonoids, Vit C, Vit E, 0.019 [34]
Thio compounds

9 Spinach Carotenoids, Flavonoids, Vit C α-tocopherol, 0.152 [34]

10 Carrot Carotenoids, Flavonoids, Vit C and E, Thio 0.060 [34]

compounds

11 White onion Carotenoids, Flavonoids, Vit C and E, Thio 0.085 [34]

compounds

12 White cabbage Carotenoids, Flavonoids, Vit C and E, Thio 0.061 [34]

compounds

14 Tomato Carotenoids, Flavonoids, Vit C and E, Thio 0.067 [34]

compounds

15 Cauliflower Carotenoids, Flavonoids, Vit C and E, Thio 0.102 [34]

compounds

16 Grape juice Anthocyanins 255.6–460 [36]

17 Coriander Monoterpenoid, [37]

18 Ginger Phenols 1870.1 [38]

19 Nigella sativa 4-terpineol Thymoquinone, Carvacrol 1.0 [39]

20 Walnut Phenolics 1320.6 [40]

Table 1.
Antioxidants obtained from various fruits, vegetables and natural sources.

The water-soluble antioxidant vitamin C, commonly referred to as ascorbic acid,

is typically found in citrus fruits and vegetables including oranges, lemons, and
tomatoes. It is a vitamin that is obtained from fruits and vegetables and serves as an
antioxidant. It is advisable to consume vitamin C-containing fruits and vegetables in
tiny, spaced-out portions rather than all at once because it demonstrates poor absorp-
tion when consumed in larger doses [46].
Vitamin E is reported to possess excellent antioxidant properties. It is a naturally
occurring, nonpolar, fat-soluble vitamin that is present in lipid-rich foods including
olives, almonds, and sunflower seeds. Vitamin E has a higher bioavailability than
vitamin C because of its solubility in fat and potential for improvement when ingested
with fatty meals [47].
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Natural Antioxidants: An Update
DOI: http://dx.doi.org/10.5772/intechopen.112462

5. Fruits and vegetable wastes as source of natural antioxidants

Producing, managing industrially, processing, preserving, and distributing

fruits and vegetables all result in the generation of waste products. Over the past few
decades, researchers have been experimenting with techniques to reuse these wastes
in order to obtain medicinal benefits [48]. Vegetable and fruit wastes consists of the
peelings, trimmings, seeds, shells, stems and pulp leftovers from juice extraction and
starch or sugar processing. Between 25 to 30% of it is trash. These discarded scalps
apparently contain more phenols and ascorbic acids than their pulp [49] likewise
preferable in their unripe form than that of ripeness. Frequently, fruit peels have 2–27
times as much antioxidants as fruit pulp [50].
Only 25% as much phenolic compounds are present in banana pulp
(232 mg/100 g) as there are in banana peels [51]. Cucumis sativus peel has been
discovered to be an excellent source of flavonoids, which are regarded to have
antioxidant potential. These wastes include a variety of bioactive components that
can be gathered and used to create both culinary preparations and pharmaceutical
preparations. The bioactive phytochemicals carotenes, tocopherols, terpenes, sterols,
and polyphenols, all of which have strong antioxidant properties, are abundant in the
tomato wastes. These natural antioxidants, which were derived from food waste, can
be used to enhance food or make useful foods [52]. The mango peel is rich in dietary
fibre, vitamin C, phenolic compounds, and carotenoids, among other antioxidants.
These compounds have been proven to affect a variety of degenerative conditions,
including cancer, Parkinson’s disease, cataracts, and Alzheimer’s disease [53]. Among
the waste materials generated by the wine industry are degradable solids. These
substances contain high levels of antioxidants, which have been shown to slow down
a number of degenerative processes and have other advantageous impacts on health.
Polyphenols make up around 6% of the waste produced by the coffee industry,
whereas tannins make up about 4% [54, 55].

6. Important characteristics of antioxidants

A chemical or antioxidant system’s main job is to stabilize the generated radical

in order to prevent or detect a chain of oxidative propagation, which minimizes
the body’s exposure to oxidative damage [56]. Gordon categorized antioxidants
according to that characteristic. Primary antioxidants (which halt a chain reaction
and scavenge free radicals) and secondary, or preventive, antioxidants fall into two
fundamental groups. A few examples of secondary antioxidant mechanisms include
the deactivation of metals, stopping the formation of unfavorable volatiles, inhibit-
ing lipid hydroperoxides, regenerating primary antioxidants, and removing singlet
oxygen. Antioxidants are therefore “those substances that, in low quantities, act by
preventing or greatly retarding the oxidation of easily oxidizable materials such as
fats” [57].

7. Conclusion

Over the previous ten years, there has been an increase in interest in studying
natural ingredients for usage in food and food products. Because natural sources
are more useful and secure to use as dietary supplements than manufactured ones,
7
Medicinal Plants - Chemical, Biochemical, and Pharmacological Approaches

researchers from all over the world are concentrating on them. Despite the fact that
there have never been any cases of harm associated with the use of synthetic antioxi-
dants, there is still a considerable desire from consumers for products that are close to
nature meal artificial antioxidants and preservatives may also result in peroxidation
of lipids and thus deterioration of quality and flavor of the food items.
Since ancient times, natural herbs, spices, and plant-based ingredients have been
employed in traditional food preparation as flavorings, fragrances, and preservatives.
A general overview of the possible benefits of several natural sources with respect-
able antioxidant capacity is what this chapter aims to deliver. The literature research
gathered here will be useful to establish the relevance, active components, antioxidant
potential and availability of various sources. This work will help the people to priori-
tize their daily requirements of natural antioxidants keeping in mind the cost-effec-
tiveness and availability of natural sources because 70–80% of the world’s population
cannot afford current supplements and pharmaceuticals.

Author details

Muhammad Alamzeb1*, Behramand Khan2, Ihsan Ullah3, Muhammad Omer3

and Adnan Rauf3

1 Department of Chemistry, University of Kotli, Kotli, Azad Jammu & Kashmir,

Pakistan

2 Department of Chemistry, Islamia College University, Peshawar, KPK, Pakistan

3 Institute of Chemical Sciences, University of Swat, Swat, Pakistan

*Address all correspondence to: [email protected]

© 2023 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of
the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided
the original work is properly cited.
8
Natural Antioxidants: An Update
DOI: http://dx.doi.org/10.5772/intechopen.112462

References

[1] Li S, Tan HY, Wang N, Zhang ZJ, [8] Zheng J, Zhou Y, Li Y, Xu DP,
Lao L, Wong CW, et al. The role of Li S, Li HB. Spices for prevention and
oxidative stress and antioxidants in treatment of cancers. Nutrients.
liver diseases. International Journal of 2016;8:495. Epub 2016/08/17
Molecular Sciences. 2015;16:26087-26124
Epub 2015/11/06 [9] Anwar H, Rahman ZU,
Javed I, Muhammad F. Effect of protein,
[2] Wang F, Li Y, Zhang YJ, Zhou Y, probiotic, and symbiotic supplementation
Li S, Li HB. Natural products for the on serum biological health markers
prevention and treatment of hangover of molted layers. Poultry Science.
and alcohol use disorder. Molecules 2012;91:2606-2613 Epub 2012/09/20
(Basel, Switzerland). 2016;21:64 Epub
2016/01/12 [10] Carocho M, Ferreira IC. A review on
antioxidants, prooxidants and related
[3] Zhou Y, Zheng J, Li S, Zhou T, controversy: Natural and synthetic
Zhang P, Li HB. Alcoholic beverage compounds, screening and analysis
consumption and chronic diseases. methodologies and future perspectives.
International Journal of Environmental Food and Chemical Toxicology: An
Research and Public Health. 2016;13:522. International Journal Published for the
Epub 2016/05/28 British Industrial Biological Research
Association. 2013;51:15-25 Epub
[4] Baiano A, Del Nobile MA. 2012/09/29
Antioxidant compounds from vegetable
matrices: Biosynthesis, occurrence, [11] Haseeb A, Ghulam H, Imtiaz M.
and extraction systems. Critical Antioxidants from natural sources.
Reviews in Food Science and Nutrition. In: Emad S, Ghada Mostafa A,
2016;56:2053-2068 Epub 2015/03/10 editors. Antioxidants in Foods and Its
Applications. Rijeka: IntechOpen; 2018.
[5] Zhang JJ, Li Y, Zhou T, Xu DP, Zhang P, p. Ch. 1
Li S, et al. Bioactivities and health benefits
of mushrooms mainly from China. [12] Asif M. Chemistry and antioxidant
Molecules (Basel, Switzerland). activity of plants containing some
2016;21:938. Epub 2016/07/23 phenolic compounds. Chemistry
International. 2015;1:35-52
[6] Deng GF, Shen C, Xu XR, Kuang RD,
Guo YJ, Zeng LS, et al. Potential of fruit [13] Ramalakshmi K, Rahath Kubra I,
wastes as natural resources of bioactive Jagan Mohan Rao L. Antioxidant
compounds. International Journal of potential of low-grade coffee beans. Food
Molecular Sciences. 2012;13:8308-8323 Research International. 2008;41:96-103
Epub 2012/09/04
[14] Shebis Y, Iluz D, Kinel-Tahan Y,
[7] Manach C, Scalbert A, Morand C, Dubinsky Z, Yehoshua Y. Natural
Rémésy C, Jiménez L. Polyphenols: antioxidants: Function and sources. Food
Food sources and bioavailability. and Nutrition Sciences. 2013;04:643-649
The American Journal of Clinical
Nutrition. 2004;79:727-747 Epub [15] Uddin G, Khan A, Alamzeb M, Ali S,
2004/04/29 Rashid MU, Alam M, et al. Biological
9
Medicinal Plants - Chemical, Biochemical, and Pharmacological Approaches

screening of ethyl acetate extract of [24] Góth L, Rass P, Páy A. Catalase

Hedera nepalensis stem. African Journal enzyme mutations and their association
of Pharmacy and Pharmacology. with diseases. Molecular Diagnosis: A
2012;6:2934-2937 Journal Devoted to the Understanding
of Human Disease through the Clinical
[16] Davies KJ. Oxidative stress: The Application of Molecular Biology.
paradox of aerobic life. Biochemical 2004;8:141-149 Epub 2005/03/18
Society Symposium. 1995;61:1-31 Epub
1995/01/01 [25] Ighodaro OM, Akinloye OA. First
line defence antioxidants-superoxide
[17] Valko M, Leibfritz D, Moncol J, dismutase (SOD), catalase (CAT)
Cronin MT, Mazur M, Telser J. Free and glutathione peroxidase (GPX):
radicals and antioxidants in normal Their fundamental role in the entire
physiological functions and human antioxidant defence grid. Alexandria
disease. The International Journal Journal of Medicine. 2018;54:287-293
of Biochemistry & Cell Biology.
2007;39:44-84 Epub 2006/09/19 [26] Gill SS, Tuteja N. Reactive oxygen
species and antioxidant machinery in
[18] Fridovich I. The biology of oxygen abiotic stress tolerance in crop plants.
radicals. Science. 1978;201:875-880 Epub Plant Physiology and Biochemistry: PPB.
1978/09/08 2010;48:909-930 Epub 2010/09/28

[19] Limón-Pacheco J, Gonsebatt ME. [27] Rayman MP. Selenium in cancer

The role of antioxidants and antioxidant- prevention: A review of the evidence and
related enzymes in protective responses mechanism of action. The Proceedings of
to environmentally induced oxidative the Nutrition Society. 2005;64:527-542
stress. Mutation Research. 2009;674:137- Epub 2005/11/30
147 Epub 2008/10/29
[28] Chen S, Wu K, Knox R. Structure-
[20] Nakamura H, Nakamura K, Yodoi J. function studies of DT-diaphorase
Redox regulation of cellular activation. (NQO1) and NRH: Quinone
Annual Review of Immunology. oxidoreductase (NQO2). Free Radical
1997;15:351-369 Epub 1997/01/01 Biology & Medicine. 2000;29:276-284
Epub 2000/10/18
[21] Dringen R, Pawlowski PG,
Hirrlinger J. Peroxide detoxification [29] Foti MC. Antioxidant properties of
by brain cells. Journal of Neuroscience phenols. The Journal of Pharmacy and
Research. 2005;79:157-165 Epub Pharmacology. 2010;59:1673-1685
2004/12/02
[30] Carr AC, Zhu BZ, Frei B. Potential
[22] Fridovich I. Superoxide radical and antiatherogenic mechanisms of ascorbate
superoxide dismutases. Annual Review (vitamin C) and alpha-tocopherol
of Biochemistry. 1995;64:97-112 Epub (vitamin E). Circulation Research.
1995/01/01 2000;87:349-354 Epub 2000/09/02

[23] Chelikani P, Fita I, Loewen PC. [31] Navarro M, Moreira I, Arnaez E,

Diversity of structures and properties Quesada S, Azofeifa G, Vargas F, et
among catalases. Cellular and Molecular al. Polyphenolic characterization and
Life Sciences: CMLS. 2004;61:192-208 antioxidant activity of Malus domestica
Epub 2004/01/28 and Prunus domestica cultivars from
10
Natural Antioxidants: An Update
DOI: http://dx.doi.org/10.5772/intechopen.112462

Costa Rica. Foods (Basel, Switzerland). of a ginger extract (Zingiber officinale).

2018;7:15. Epub 2018/02/02 Food Chemistry. 2007;102:764-770

[32] Nantitanon W, Yotsawimonwat S, [39] Burits M, Bucar F. Antioxidant

Okonogi S. Factors influencing activity of Nigella sativa essential
antioxidant activities and total oil. Phytotherapy research: PTR.
phenolic content of guava leaf extract. 2000;14:323-328 Epub 2000/08/05
LWT – Food Science and Technology.
2010;43:1095-1103 [40] Oliveira I, Sousa A, Ferreira IC,
Bento A, Estevinho L, Pereira JA. Total
[33] Fernández-López JA, Almela L, phenols, antioxidant potential and
Obón JM, Castellar R. Determination of antimicrobial activity of walnut (Juglans
antioxidant constituents in cactus pear regia L.) green husks. Food and Chemical
fruits. Plant Foods for Human Nutrition Toxicology: An International Journal
(Dordrecht, Netherlands). 2010;65:253- Published for the British Industrial
259 Epub 2010/09/03 Biological Research Association.
2008;46:2326-2331 Epub 2008/05/02
[34] Ou B, Huang D,
Hampsch-Woodill M, Flanagan JA,
[41] Parr AJ, Bolwell GP. Phenols in the
Deemer EK. Analysis of antioxidant
plant and in man. The potential for
activities of common vegetables
possible nutritional enhancement of the
employing oxygen radical absorbance
diet by modifying the phenols content
capacity (ORAC) and ferric reducing
or profile. Journal of the Science of Food
antioxidant power (FRAP) assays:
and Agriculture. 2000;80:985-1012
A comparative study. Journal of
Agricultural and Food Chemistry.
[42] Quintavalla S, Vicini L.
2002;50:3122-3128 Epub 2002/05/16
Antimicrobial food packaging in meat
[35] Oloyede O, Franco J, Roos D,
industry. Meat Science. 2002;62:373-380
Rocha JB, Linde Athayde M, Boligon A. Epub 2002/11/01
Antioxidant properties of ethyl acetate
[43] Tain Y-L, Hsu C-N. Oxidative stress-
fraction of unripe pulp of Carica papaya
in mice. Journal of Microbiology, induced hypertension of developmental
Biotechnology and Food Sciences. origins. Preventive Aspects of
2011;1:409-425 Antioxidant Therapy. 2022;11:511

[36] Kim MJ, Jun JG, Park SY, Choi MJ, [44] Rashid MU, Alamzeb M, Ali S,
Park E, Kim JI, et al. Antioxidant Khan A, Semaan D, Igoli J, et al. A new
activities of fresh grape juices ceramide along with eight known
prepared using various household compounds from the roots of artemisia
processing methods. Food Science and incisa Pamp. Records of Natural
Biotechnology. 2017;26:861-869 Epub Products. 2015;9:3-294
2017/07/12
[45] Farbstein D, Kozak-Blickstein A,
[37] Wangensteen H, Samuelsen AB, Levy AP. Antioxidant vitamins and their
Malterud KE. Antioxidant activity in use in preventing cardiovascular disease.
extracts from coriander. Food Chemistry. Molecules. 2010;15:8098-8110
2004;88:293-297
[46] McGhie TK, Walton MC. The
[38] Stoilova I, Krastanov A, Stoyanova A, bioavailability and absorption of
Denev P, Gargova S. Antioxidant activity anthocyanins: Towards a better
11
Medicinal Plants - Chemical, Biochemical, and Pharmacological Approaches

understanding. Molecular Nutrition & The chemical composition of exhausted

Food Research. 2007;51:702-713 Epub coffee waste. Industrial Crops and
2007/05/30 Products. 2013;50:423-429

[47] Daniel JW. Metabolic aspects [55] Teixeira A, Baenas N,

of antioxidants and preservatives. Dominguez-Perles R, Barros A, Rosa E,
Xenobiotica: The Fate of Foreign Moreno DA, et al. Natural bioactive
Compounds in Biological Systems. compounds from winery by-products as
1986;16:1073-1078 Epub 1986/10/01 health promoters: A review. International
Journal of Molecular Sciences.
[48] Mirabella N, Castellani V, Sala S. 2014;15:15638-15678 Epub 2014/09/06
Current options for the valorization
of food manufacturing waste: A [56] Namiki M. Antioxidants/
review. Journal of Cleaner Production. antimutagens in food. Critical Reviews
2014;65:28-41 in Food Science and Nutrition.
1990;29:273-300 Epub 1990/01/01
[49] Goulas V, Manganaris GA. Exploring
the phytochemical content and the [57] Norma Francenia S-S, Raúl S-C,
antioxidant potential of Citrus fruits Claudia V-C, Beatriz H-C. Antioxidant
grown in Cyprus. Food Chemistry. compounds and their antioxidant
2012;131:39-47 mechanism. In: Emad S, editor.
Antioxidants. Rijeka: IntechOpen; 2019.
[50] Fatemeh S, Saifullah R, Abbas FM, p. Ch. 2
Azhar ME. Total phenolics, flavonoids
and antioxidant activity of banana pulp
and peel flours: Influence of variety and
stage of ripeness. International Food
Research Journal. 2012;19:1041-1046

[51] Someya S, Yoshiki Y, Okubo K.

Antioxidant compounds from bananas
(Musa Cavendish). Food Chemistry.
2002;79:351-354

[52] Baiano A. Recovery of biomolecules

from food wastes: A review. Molecules
(Basel, Switzerland). 2014;19:14821-
14842 Epub 2014/09/19

[53] Ayala-Zavala JF, Rosas-Domínguez C,

Vega-Vega V, González-Aguilar GA.
Antioxidant enrichment and
antimicrobial protection of fresh-cut
fruits using their own byproducts:
Looking for integral exploitation. Journal
of Food Science. 2010;75:R175-R181 Epub
2011/05/04

[54] Pujol D, Liu C, Gominho J,

Olivella MÀ, Fiol N, Villaescusa I, et al.
12