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Enzymes
Question Paper 3
Level IGCSE
Subject Biology
Exam Board CIE
Topic Enzymes
Sub-Topic
Paper Type Alternative to Practical
Booklet Question Paper 3
Time Allowed: 36 minutes
Score: /30
Percentage: /100
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1 Catalase, an enzyme, is present in all living cells including those of potato and liver. It
speeds up the breakdown of hydrogen peroxide as shown by the equation:
catalase
hydrogen peroxide oxygen + water
The oxygen is given off as a gas which can be collected over water, as shown in Fig. 1.1.
1
2
3
4
5
6
7
8
9
10
cm3
hydrogen
peroxide
potato or
liver tissue
Fig. 1.1
Two different tissues, potato and liver, were used for this investigation. Samples, each of one
gram, were prepared from both tissues. Some of the samples were left raw and others were
boiled. Some samples were left as one cube and others were chopped into small pieces as
shown in Table 1.1 on page 4.
2 cm3 hydrogen peroxide was added to each sample. The volume of oxygen produced in five
minutes was collected in the measuring cylinders, as shown in Table 1.1.
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Table 1.1
sample A B
treatment
raw raw boiled boiled
results for
potato 2 2 2 2
4 4 4 4
6 6 6 6
8 8 8 8
10 10 10 10
cm3 cm3 cm3 cm3
results for
liver 2 2 2 2
4 4 4 4
6 6 6 6
8 8 8 8
10 10 10 10
cm3 cm3 cm3 cm3
(a) (i) Complete Table 1.2, by reading the values for oxygen collected in the measuring
cylinders in Table 1.1.
Table 1.2
volume of oxygen collected from each sample / cm3
tissue
A B C D
potato
liver
[2]
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(ii) Plot the volumes of oxygen collected from the samples as a bar chart on the grid.
[4]
(iii) Describe the difference in results between sample A for potato and sample A for
liver.
[2]
(iv) There is a difference between the samples for A and B for liver.
Suggest an explanation for this difference.
[2]
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(b) State the importance of samples C and D in this investigation.
[1]
(c) Suggest how you could test that the gas given off was oxygen.
[1]
[Total 12]
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2 A protein is used to hold other chemicals onto the clear plastic backing of photographic film,
as shown in Fig. 1.1.
clear plastic
chemicals of photographic film backing
held in a layer of protein
Fig.1.1
Trypsin is an enzyme which will digest the protein so that the coating on the photographic
film is removed and the film becomes clear.
Table 1.1 shows the results obtained by two students who investigated the effect of pH on
the activity of this enzyme. They made up the solutions, set up the experiment and timed
how long the enzyme took to digest the protein and clear the film.
Table 1.1
time for the protein to be digested / mins
pH
student 1 student 2
2 12.0 14.0
4 8.0 9.0
6 2.0 3.0
8 0.5 1.0
10 8.0 9.0
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(a) (i) Plot the results obtained by student 2 in the form of a suitable graph.
[5]
(ii) Describe and explain the effect of pH on the activity of the enzyme.
[5]
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(b) (i) Suggest reasons for the difference in the results for the two students.
[3]
(ii) If you were to carry out this investigation, describe what steps you would take to
ensure that your results were as reliable and valid as possible.
[5]
[Total:18]
