BASIC BIOTECHNOLOGY
LONG QUIZ
Direction: Read the items carefully. Choose the letter of the correct answer.
1. Capacity of mature cell to return to meristematic condition and development of a new growing point.
a. Dedifferentiation c. Totipotency
b. Competency d. Regeneration
2. Ability of a cell or tissue or organ to grow and develop into a fully differentiated organism.
a. Dedifferentiation c. Totipotency
b. Competency d. Regeneration
3. The art and science of plant multiplication in vitro (outside of the organism)
a. Micropropagation c. Shoot tip culture
b. Grafting d. Callus culture
4. The following are the methods or procedure use in cell lysis except
a. Chemical c. Mechanical
b. Enzymatic d. Inorganic
5. It helps break down the cell wall membrane
a. Detergent c. Cologne
b. Zonrox d. Mouth wash
6. What can be the substitute for phenol and chloroform in classroom labs.
a. Salt c. Downy
b. Liquid Detergent d. Joy
7. The following are the apparatus used to check the extracted DNA except
a. Capillary electrophoresis c. Vertical electrophoresis
b. Spectophotometer d. Gel electrophoresis
8. Genomic extraction should result in ______________ in the very high base pair range of a gel
electrophoresis.
a. Blunt band c. Bright band
b. Straight band d. Stripe band
9. The recombinant DNA enters the host cell and proliferate
a. Recombination c. Transpiration
b. Transformation d. Reconciliation
c.
10. A method involves the termination of newly synthesized DNA Strands at specific sites, the used of
restricted enzymes
a. Chain-termination method c. Restriction method
b. Maxam- Gilbert chemical degradation method d. Cutting method
11. A method uses a specialized purpose, such as chemical cleavage protocol
a. Chain-termination method c. Restriction method
b. Maxam- Gilbert chemical degradation method d. Cutting method
12. A technique used in molecular biology, by detection of RNA in a sample
a. Northern blotting c. Western blotting
b. Southern blotting d. Eastern blotting
13. A common method to detect and analyze proteins.
a. Northern blotting c. Western blotting
b. Southern blotting d. Eastern blotting
14. A method used in molecular biology for detection of a specific DNA sequence
a. Northern blotting c. Western blotting
b. Southern blotting d. Eastern blotting
15. If F1 is crossed with dominant parent all progenies will be
a. Dominant c. Haploid
b. Recessive d. Equal proportion
16. If F1 is crossed with recessive parent all progenies will be
a. Dominant c. Haploid
b. Recessive d. Equal Proportion
17. The cross of F1 offspring with one of its parents
a.Crisscross c. Backcross
b. Hybrid d. Inbreed
18. No room for punctuation is a property of genetic code that means
a. Comma less c. Nonoverlapping
b. Universal d. Polarity
19. A dictionary that corresponds with sequence of nucleotides and amino acids
a. Genetic code c. Gene Bank
b. Genetic Library d. Gene structure
20. A series of three nucleotides that determine a single
a. Proteins c. Vitamins
b. Sugar d. Amino acids
Identification
Direction: Read the items carefully. Identify what is being asked.
Haploid 1. Contains a single set of chromosomes or half of chromosome
Auxin 2. Stimulates root development
Cytokinin 3. Stimulates shoot development
4. MS medium stands for Murashige and Skoog
Sterile 5. Inability of an organism to produced functional gametes
Cell lysis/ Lysis 6. Step refers to the breaking of the cell wall & cellular membranes
Precipitation Series of step where DNA is separated from the rest of cellular components
Gene library 8. A large collection of cloned DNA sequence from a single genome
Southern blotting9. Named after DR. Southern
Fred Sanger 10. He invented the chain-termination method
Maxam Gilbert 11. He invented the chemical degradation method
60 12. How many codons codes
21 13. How many amino acids
Backcross Breeding 14. A cross between a hybrid (F1) and one of its parents
Backcross ratio 15. The ratio of progenies produced during back cross is called
____________ 16. ANAA stands for (Alpha Naphthalene Acetic Acid)
_____________17. NAA stands for (Naphthalene Acetic Acid)
_____________18. IAA stands for (Indole – 3-Acetic Acid)
_____________19. IBA stands for (Indole – 3 – Butyric Acid)
_____________20. BA stands for (Benzyl adenine)
Enumeration
Direction: Read the items carefully. Give what is being asked.
1-3. Basic steps of DNA extraction
Cell Lysis
Protein Removal
DNA Precipitation
4-7. Basic media that frequently used in Tissue culture
MS-Murashige and Skoog, LS- Linsmaier and Skoog
B5 (Gamborg),
8-10. Give at least 3 options used in storing of DNA
Salt solution, Alcohol, Freezing, Dried
11-15. Steps in Micropropagation
Selection & preparation of the mother plant
Initiation of culture
Multiplication
Rooting
Transfer to soil
16-20. Amino acids
1. I n p l a n t c u l t u r e s
whole plants. Answer: Regeneration
2. Most popular among the media
3. It is a media with high levels of nitrates, potassium and ammonium compared to other medium formula
4. This media used for the purpose of organogenesis, callus culture and cell suspension Answer: Linsmaier and
Skoog
5. This media used for the purpose of protoplast culture Answer: Gamborg Medium
6. This media used for establishing in vitro anther culture Answer: Nitsch and Nitsch medium
7. What chemical added to make cut for the fragment at Adenine position Answer: Dimethyl sulfate + diluted HCL
8. What chemical added to make cut for the fragment at Cytosine position Answer: Hydrazine+ Piperidine+ NaCL
9. The following are the methods or procedure use in cell lysis except
c. Chemical c. Mechanical
d. Enzymatic d. Inorganic
10. It helps break down the cell wall membrane
c. Detergent c. Cologne
d. Zonrox d. Mouth wash
11. What can be the substitute for phenol and chloroform in classroom labs.
c. Salt c. Downy
d. Liquid Detergent d. Joy
12. The following are the apparatus used to check the extracted DNA except
A. Capillary electrophoresis c. Vertical electrophoresis
B. Spectophotometer d. Gel electrophoresis
13. A biological unit that determines an organism’s inherited characteristics.
a. Gene
14. Any technique that makes use of organisms (or parts thereof) to make or modify products
a. Biotechnology
15. Also known as transgenic crop—plant that has a novel combination of genetic material obtained through the use
of modern biotechnology
a. Genetically modified crops
16. The following the fundamental abilities of plants except
a. Differentiation c. Genetics
b. Competency d. Environmental
17. The following are the factors affecting Plant Tissue Culture except
a. Genetics c. Environmental factors
b. Growth Media d. Explant Source
18. A technique used in molecular biology, by detection of RNA in a sample
a. Northern blotting c. Western blotting
b. Southern blotting d. Eastern blotting
19. A common method to detect and analyze proteins.
a. Northern blotting c. Western blotting
b. Southern blotting d. Eastern blotting
20. A method used in molecular biology for detection of a specific DNA sequence
a. Northern blotting c. Western blotting
b. Southern blotting
20.
