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Class No 6 : HISTO- & CYTO TECHNIQUES

EXAMINATION CONTENT AREAS:

Sample types, Processing, Embedding, Cutting, stains, Frozen section,
Sample storage and disposal, Molecular Pathology

Histo:

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1. Inspect the specimen with naked eyes & note description is

A. Tissue Processing
B. Surgery
C. Grossing

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D. Visual Description

Answer : C. Grossing
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2. Which knife is used for Hard tissue?

A. plane wedge
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B. Tool edge
C. Biconcave
D. planoconcave
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Answer : B. Tool edge

3. Histopathology slide store for future references at least up to ?

A. 1 year
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B. 3 days
C. 15 days
D. 10 year

Answer : D. 10 year

4. Which of the following is write step for histological technique?

A. Grossing - Mounting - staining

B. Dehydration - Cleaning - Embedding - Sectioning
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C. Clearing - Embedding - Decalcification - mounting
D. All above are wrong

Answer : B. Dehydration - Cleaning - Embedding - Sectioning

5. Increasing grade of alcohol used for ?

A. Hydration
B. Dehydration
C. Clearing
D. Embedding

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Answer : B. Dehydration

6. Which of the following fixative used for Electron Microscopy?

A. Formaldehyde

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B. Potassium dichromate
C. Picric Acid
D. Osmium tetroxide
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Answer : D. Osmium tetroxide

7. Which of the following are not dehydrating agent?

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A. Alcohol
B. Acetone
C. Xylene
D. Dioxane
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Answer : C. Xylene

8. Which of the following is mix with paraffin?

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A. Alcohol
B. Water
C. Xylene
D. Acetone

Answer : C. Xylene

9. Rough cutting should be done at?

A. 20um
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B. 10um
C. 5um
D. 3um

Answer : 10um

10. Which method required for histopathology disposable blade sharping?

a. Honing
b. Stropping
c. Honing & stroppig

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d. None of above

Answer : D. None of above

11. Which hematoxylin is used for demonstration of glycogen?

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A. PAS
B. Harris Hematoxylin
C. Mayer’s Hematoxylin
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D. All of above

Answer : D. Mayer’s Hematoxylin

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12. Which of the following not demonstrate through frozen section ?
A. Rapid diagnosis of cancer
B. EHC
C. Protein
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D. Fat

Answer : C. protein

13. In CNS nissel substance mostly contain?

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A. RNA
B. Protein
C. Enzymes
D. Carbohydrate

Answer : A. RNA

14. The ripening of stain due to?

A. Oxidation and Reduction
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B. Oxidation
C. Ultra-oxidation
D. Reduction

Answer : B. Oxidation

15. Which of the following methods could be used to remove mercury pigment, a
fixation artifact?
a. Treat the specimen with an iodine solution followed by bleaching with
sodium thiosulfate

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b. Treat the specimen with saturated alcoholic picric acid
c. Treat the specimen with 10% ammonium hydroxide in 70% ethyl alcohol
d. Treat the specimen with 1% acid alcohol

Answer : A. Treat the specimen with an iodine solution followed by bleaching with

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sodium thiosulfate

16. Section cutting best?

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A. At 37 degrees
B. At 4 degrees
C. After warming the block
D. At RT
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Answer : D. At RT

17. Tissue appear cloudy & opaque due to?

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A. Over Dehydration
B. Under dehydration
C. Inaccurate staining
D. Insufficient Clearing
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Answer : D. Insufficient Clearing

18. Masson-Fontana stain used for?

a. Iron
b. Copper
c. Melanin
d. Fat

Answer : C. Melanin
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19. Which stain used for mainly fungi in histopathology?

a. Grocott’s
b. Gram
c. LCB
d. All of above

Answer : A. Grocott’s

20. L shaped mould used for?

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a. Tissue cutting
b. Knife sharping
c. Embedding
d. Infiltration

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Answer : C. Embedding

21. Clearing step in tissue processing also know as?

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a. Refresh
b. Alcoholization
c. Dealcoholization
d. Transparent
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Answer : C. Dealcoholization

22. Black colour pigment leaved by which fixative solution?

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a. Glutaraldehyde
b. Mercuric chloride
c. Formalin
d. All of above
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Answer : B. Mercuric chloride

23. Bone marrow is fixed in ?

a. Zenker fluid
b. Formalin
c. Carnoy’s fluid
d. Glutaraldehyde

Answer : B. Formalin
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24. Which method is expensive to check decalcification?

a. Needling
b. Chemical
c. Biological
d. X-ray

Answer : D. X-ray

25. Acetone is a?

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a. Clearing agent
b. Dehydrating agent
c. Embedding media
d. None of above

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Answer : A. Clearing agent

26. Which hematoxylin used for endocrine cells?

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a. Mayer’s
b. Lead
c. Iron
d. Harris
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Answer : B. Lead

27. Which stain not used for fat’s?

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a. Oil red o
b. Sudan black b
c. Dichromate acid hematin
d. Fite wade
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Answer : D. Fite wade

28. Which type of mounting media used commonly?

a. Aqueous mounting media
b. Resinous mounting media
c. Natural mounting media
d. Synthetic mounting media

Answer : B. Resinous mounting media

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29. S-100 protein marker used for?

a. Lymphoma
b. Nerve cell
c. Breast cancer
d. Prostate

Answer : B. Nerve cell

30. Thyroglobulin is marker for

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a. Thyroid cancer
b. Multiple myeloma
c. Prostate
d. All of above

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Answer : A. Thyroid cancer

31. How many wax bath present in automatic tissue processor?

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a. 1
b. 2
c. 3
d. 4
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Answer : A. 1

32. What should be the ratio between the volume of the tissue and the fixative
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a. 1 ratio 5
b. 1 : 10
c. 1 ratio 20
d. 1 ratio 100
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Answer C. 1 ratio 20

33. What is the usual concentration of the commercial formaldehyde available

a. 7 to 10%
b. 17 to 27%
c. 37 to 40%
d. 40 to 50%

Answer : C. 37 to 40%
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34. Bouin's fluid is yellow because of the presence of.

a. Chloroform
b. Picric acid
c. Formaldehyde
d. Iodine

Answer : B. Picric acid

35. Which of the following acid may be used for decalcification ?

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a. 25 % sulfuric acid
b. 5 % nitric acid
c. 20 % hydrochloric acid
d. 30% orthophosphoric acid

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Answer : B. 5 % nitric acid

36. Tissue processing unit are also known as ?

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a. HistoKinette
b. Histomat
c. Histobath
d. Histogram
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Answer : A. HistoKinette

37. The section cutting machine is known as

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a. Microtome
b. Histomate
c. Histobath
d. Histogram
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Answer : A. Microtome

CYTO

38. What is the primary stain used in cytopathology to visualize cellular

morphology?
a. Hematoxylin and Eosin
b. Papanicolaou stain
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c. wright-Giemsa stain
d. Gram stain

Answer : B. Papanicolaou stain

39. Which cell type characteristic of a benign squamous epithelial lesion in

cervical cytology?
a. Koilocytes
b. Dyskeratocytes
c. Superficial squamous cells

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d. Parabasal cells

Answer : C. Superficial squamous cells

40. Which virus is commonly associated with koilocytosis in cervical cytology?

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a. Herpes simplex virus
b. Epistein-Barr virus
c. Human papillomavirus
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d. Cytomegalovirus

Answer : C. Human papillomavirus

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41. What cytologic feature is typically observed in adenocarcinoma of the lung?
a. Large, pleomorphic cells with scant cytoplasm
b. Glandular formations with mucin production
c. Small, dark nuclei with dense chromatin
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d. Reed-sternberg cells

Answer : B. Glandular formations with mucin production

42. Which cytological finding is most suggestive of bacterial vaginosis?

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A. Clue cells
B. Candida pseudohyphae
C. Trichomonas Vaginalis
D. Reactive lymphocytes

Answer : A. Clue cells

43. What is the typical nuclear feature of malignant cells in cytology?

a. Nuclear enlargement with hyperchromasia
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b. Small nuclei with dense chromatin abnormality
c. Binucleation without chromatin abnormality
d. Cytoplasmic vacuolization

Answer : A. Nuclear enlargement with hyperchromasia

44. In fine-needle aspiration (FNA) cytology of a thyroid nodule, which finding is

most suggestive of a papillary thyroid carcinoma?

a. Colloid

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b. Anisonucleosis
c. Psammoma bodies
d. Hurthle cells

Answer : C. Psammoma bodies

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45. Which finding on cervical cytology for high-grade squamous intraepithelial
lesion (HSIL)?
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a. Koilocytes
b. Multinucleation
c. Increased nuclear-to-cytoplasmic ratio
d. Parabasal cells
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Answer : C. Increased nuclear-to-cytoplasmic ratio

46. Which organism is commonly identified on cytology by the presence of

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pseudohyphae and budding yeast forms?

a. Trichomonus vaginalis
b. Gardinerella vaginalis
c. Candida albicans
d. Chlamydia trachomatis
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Answer : C. Candida albicans

47. What is the cytologic hallmark of a granulomatous inflammation?

a. Multinucleated giant cells
b. Necrosis
c. Reactive lymphocytes
d. Basophilic stippling
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Answer: A. Multinucleated giant cells

48. Which of the following is the most common site for FNAC?
a. Liver
b. Thyroid
c. Breast
d. Lymph node

Answer : B. Thyroid

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49. Which type of cytology is most commonly used for cervical cancer
screening?
a. Urine cytology
b. Sputum cytology
c. Bronchial washings

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d. Pap smear

Answer : D. Pap smear

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50. Which cytological feature benign from malignant breast lesions in FNA
samples:
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a. Cellularity
b. Pleomorphism
c. Myoepithelial cells
d. Hyperchromasis
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Answer : C. Myoepithelial cells

51. Which of the following is a common feature of follicular neoplasms of the

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thyroid on cytology?
a. Psammoma bodies
b. Microfollicles
c. Papillary structures
d. Hurthle cells

Answer : B. Microfollicles
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52. In cervical cytology, what does the presence of atypical glandular cells (AGC)
suggest?
a. Low-grade squamous intraepithelial lesion (LSIL)
b. Adenocarcinoma or precancerous glandular lesions
c. Reactive endocervical cells
d. Atrophic changes

Answer : B. Adenocarcinoma or precancerous glandular lesions

53. Which diagnostic feature differentiates small cell lung carcinoma from

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non-small cell lung carcinoma
a. High nuclear-to-cytoplasmic ratio
b. Mucin production
c. Presence of psammoma bodies
d. Multinucleated giant cells

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Answer : A. High nuclear-to-cytoplasmic ratio
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54. What cytological feature is typically seen in cases of metastatic melanoma?
a. Melanin pigment within cells
b. Reed-sternberg cells
c. Dyskeratosis
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d. Psammoma bodies

Answer : A. Melanin pigment within cells

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55. What type of sample is typically obtained through fine needle Aspiration
cytology (FNAC)?
a. Tissue biopsy
b. Body fluids
c. Solid mass lesions
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d. Blood samples

Answer : C. Solid mass lesions

MD Monowar Hossain
DMLT(Lab medicine), B.Sc(Food and Nutrition Science)
MLT ( Certified by Dubai Health Authority-DHA)
Medical technologist - ZH Sikder Women's Medical College & Hospital