Table

Table of
of Contents
Contents

Introduction
Introduction

Analytical Technologies
Analytical Technologies
Glyceride Analysis
Glyceride Analysis
Triglyceride Analysis

Triglyceride AnalysisFatty Acids

Poly-Unsaturated

Antioxidant Additives
Poly-Unsaturated Fatty Acids

References
Antioxidant Additives

Column Selection Guide

Chromatography for Foods and Beverages

Fats and Oils Analysis
Applications Notebook
Solvable Solutions for Hydrophobic Compounds
Table of Contents

Introduction

Analytical Technologies

Glyceride Analysis
Fats, Oils, and Other Lipids
Triglyceride Analysis

Poly-Unsaturated Fatty Acids

Antioxidant Additives

References

Introduction
Many compounds are insoluble in water but are very soluble in organic
solvents. These biological compounds are referred to as lipids. Many
different types of lipids exist, often grouped together into families for
convenience. To the food scientist, perhaps the most familiar lipids are
fats and oils. Both fats and oils have similar structures, being composed
of a glycerol backbone with a variety of fatty acids attached. Depending
upon which fatty acids are present, the result can be either a solid (fat) or
liquid (oil) at room temperature. Fats and oils can be obtained from either
animals (e.g., butter, lard, fish oil) or plants (e.g., peanut butter, vegetable
shortening, olive oil).
Table of Contents

Introduction

Analytical Technologies

Glyceride Analysis
Fats, Oils, and Other Lipids
Triglyceride Analysis

Poly-Unsaturated Fatty Acids

Antioxidant Additives

References
The type of fatty acids present not only affects the physical form of the
fat/oil, but also its health benefits. Saturated fats, including meat fats, milk
fat, butter, lard, coconut oil, and palm oil, are purported to be unhealthy
and associated with increased risk of cardiovascular disease. Unsaturated
fats occur in either trans- or cis-forms. Trans-fats, which are typically man-
made and formed by the partial hydrogenation of oils, are thought not to
be healthy. Fats that contain multiple cis-forms, e.g., omega-3 fatty acids,
typically obtained from the consumption of fish oil or flax-seed oil, may be
associated with health benefits.
Lipids are often measured using gas chromatography (GC) with flame
ionization detection or mass spectrometry. Although GC offers excellent
resolution it does require that the lipids be derivatized in order to render
them volatile prior to analysis.
Lipids can also be separated using HPLC techniques. However, as lipids
typically lack a chromophore, UV absorbance detection cannot be used
effectively. Charged aerosol detection is ideal for a wide range of non-
volatile analytes, whether a chromophore is present or not.
Table of Contents

Introduction

Analytical Technologies

Glyceride Analysis

Triglyceride Analysis
Analytical Technologies
Poly-Unsaturated Fatty Acids

Antioxidant Additives

References

High-Performance
Liquid Chromatography
Thermo Scientific™ Dionex™ UltiMate™ 3000 UHPLC+
systems offer excellent chromatographic performance,
operational simplicity and unrivaled flexibility. Choose
from a wide range of standard and unique specialty
detectors to extend your laboratory’s analytical
capabilities.
 Analytical Technologies

Table of Contents

Introduction UltiMate 3000 UHPLC+ Systems

Analytical Technologies
Best-in-class HPLC systems for all your chromatography needs
Glyceride Analysis
UltiMate 3000 UHPLC+ Systems provide excellent chromatographic performance while maintaining easy, reliable operation. The basic and standard
Triglyceride Analysis analytical systems offer ultra HPLC (UHPLC) compatibility across all modules, ensuring maximum performance for all users and all laboratories.
Covering flow rates from 20 nL/min to 10 mL/min with an industry-leading range of pumping, sampling, and detection modules, UltiMate 3000
Poly-Unsaturated Fatty Acids
UHPLC+ Systems provide solutions from nano to semipreparative, from conventional LC to UHPLC.
Antioxidant Additives Superior chromatographic performance
References • UHPLC design philosophy throughout nano, standard analytical, and rapid separation liquid chromotography (RSLC)
• 620 bar (9,000 psi) and 100 Hz data rate set a new benchmark for basic and standard analytical systems
• RSLC systems go up to 1000 bar and data rates up to 200 Hz
• ×2 Dual System for increased productivity solutions in routine analysis
• Fully UHPLC compatible advanced chromatographic techniques
• Thermo Scientific™ Dionex™ Viper™ and nanoViper™ fingertight fittings—the first truly universal, fingertight fitting system even at UHPLC pressures

Learn more at www.thermoscientific.com/liquidchromatography

 Analytical Technologies

Table of Contents

Introduction UltiMate 3000 UHPLC+ Systems

Analytical Technologies
We are uniquely focused on making UHPLC technology available to all users, all laboratories, and for all analytes.
Glyceride Analysis

Triglyceride Analysis

Poly-Unsaturated Fatty Acids

Antioxidant Additives

References

Rapid Separation LC Systems Standard LC Systems

The extended flowpressure footprint of the RSLC system provides the performance for ultrafast Choose from a wide variety of standard LC systems for demanding LC applications at nano,
high-resolution and conventional LC applications. capillary, micro, analytical, and semipreparative flow rates.

RSLCnano Systems Basic LC Systems

The Rapid Separation nano LC System (RSLCnano) provides the power for high resolution and UltiMate 3000 Basic LC Systems are UHPLC compatible and provide reliable, high performance
fast chromatography in nano, capillary, and micro LC. solutions to fit your bench space and your budget.
 Analytical Technologies

Table of Contents

Introduction Standard HPLC Detectors

Analytical Technologies
UltiMate 3000 Variable Wavelength • Front panel access for quick and easy lamps and flow cells changes
Glyceride Analysis Detectors • Automated qualification monitoring for full regulatory compliance
Triglyceride Analysis The Thermo Scientific Dionex UltiMate 3000 VWD-3000 is a variable • Large front panel display for monitoring the detector status even from
wavelength detector (VWD) series for industry leading UV-Vis detection. a distance
Poly-Unsaturated Fatty Acids The forward optics design and wide range of available flow cells ensure • Maximize uptime using predictive performance–based on monitoring
optimal performance over a flow rate range of five orders of magnitude. the life cycle of detector lamps
Antioxidant Additives
Automated qualification, performance optimization, and instrument wellness
References monitoring deliver maximum uptime, simplify work-flow, and give you full • The detector can be upgraded with the Thermo Scientific Dionex
confidence in your analytical results. The detector is available in a standard pH/Conductivity Monitor (PCM-3000) for accurate and precise pH-
100 Hz (VWD-3100) and a 200 Hz Rapid Separation version (VWD- and conductivity monitoring
3400RS) for the most challenging UHPLC applications. • Unique 45 nL ultra-low dispersion UV monitor for dispersion-free UV
detection in LC/MS
High-Performance UV-Vis Detection
• The VWD-3400RS variant provides data collection rates of up to 200
Hz for optimal support of today’s and tomorrow’s UHPLC separations
• The VWD-3100 standard detector operates at up to 100 Hz data rate
for optimum support of 62 MPa (9000 psi) UltiMate 3000 Standard
systems
• Superior detection of trace analytes with low noise (< –2.0 μAU) and
drift (< 100 μAU/h)
• The detector’s large linearity range of up to 2.5 AU is ideal for appli-
cations with widely varying analyte concentrations
• Up to four absorption channels (VWD-3400RS) and spectral scans
UltiMate 3000 VWD-3400 Variable Wavelength Detector.
support effective method development
• Active temperature control of optics and electronics for data acquisi-
tion independent of ambient conditions

Learn more at www.thermoscientific.com/liquidchromatography

 Analytical Technologies

Table of Contents

Introduction Standard HPLC Detectors

Analytical Technologies
UltiMate 3000 Diode Array and • The detector can be upgraded with the UltiMate PCM 3000 for accu-
Glyceride Analysis Multiple-Wavelength Detectors rate monitoring pH gradients
Triglyceride Analysis The Thermo Scientific Dionex UltiMate DAD 3000 detector is a high-res- • Excellent reliability and reproducibility with low baseline drift (typically
olution, 1024-element diode array detector (DAD) available in Rapid < 500 μAU/h)
Poly-Unsaturated Fatty Acids Separation (200 Hz) and Standard (100 Hz) versions. It operates with • Simplified routine maintenance with front access to pre-aligned cells
the Thermo Scientific™ Dionex™ Chromeleon™ Chromatography Data and lamps
Antioxidant Additives
System (CDS) software to provide a variety of spectra views, including • ID chips on flow cells and lamps for identification and life-span moni-
References 3-D plotting and automated chromatogram handling. The high resolution toring
and low-noise performance of the DAD-3000 family makes it ideal for
the most sensitive and accurate library searches and peak purity analyses. • Chromeleon CDS software for full control and flexible data handling
The detector is also available as a multiple wavelength detector (MWD) in • Front-panel display for easy monitoring of detector status to maximize
Standard (100 Hz) and Rapid Separation (200 Hz) versions. uptime
• Data collection at up to 200 Hz using a maximum of eight sin- • Flow cells for semi-micro, semi-analytical, analytical, and semi-prepar-
gle-wavelength data channels and one 3-D field (3-D only with ative applications
DAD-3000 (RS)) for best support of ultrafast separations • Flow cells available in stainless steel and biocompatible versions
• Standard versions operate at up to 100 Hz data collection rate for
optimum support of 62 MPa (9000 psi) UltiMate 3000 Standard
systems
• Accurate compound confirmation with a 1024-element, high resolu-
tion photodiode array
• Flexibility in both UV and Vis applications with 190–800 nm wave-
length range
• Low-noise over the full spectral range using deuterium and tungsten
lamps
• Fast and accurate wavelength verification using a built-in holmium
oxide filter UltiMate 3000 DAD-3000 Diode Array Detector

Learn more at www.thermoscientific.com/liquidchromatography

 Analytical Technologies

Table of Contents

Introduction Standard HPLC Detectors

Analytical Technologies
RefractoMax 521 Refractive Index Detector • The extended flow rate range from 1 mL/min up to 10 mL/min and
Glyceride Analysis the operating range of 1.00 to 1.75 RIU enable the use of this detec-
The Thermo Scientific RefractoMax 521 Refractive Index Detector from
ERC Inc. This detector, in combination with the UltiMate 3000 system, is tor for a wide range of applications
Triglyceride Analysis
the right choice for the isocratic analysis of sugars, polymers, and fatty • Applications include the analysis of all compounds with low UV-Vis
Poly-Unsaturated Fatty Acids acids. It features fast baseline stabilization and excellent reproducibility, activity, such as alcohols, mono- and polysaccharides, esters, fatty
combined with high sensitivity. The RefractoMax 521 is fully controlled by acids, or polymers
Antioxidant Additives
the Chromeleon CDS, and can also operate in stand-alone mode. • An Auto Set-up function automates purging, equilibration, autozero,
References • The detector is highly sensitive and applicable universally. It provides and the control baseline stability and noise
very stable baselines with a drift of 0.2 µRIU/h and a noise specifica- • Operation with Chromeleon CDS makes the detector easy to use and
tion of 2.5 nRIU or less ensures maximum productivity in instrument control, data processing,
• The optical bench, thermostatically regulated from 30 °C to and reporting of results
55 °C, and the superior signal-to-noise ratio ensure highly precise
measurement results

RefractoMax 521 Refractive Index Detector

Learn more at www.thermoscientific.com/RI

 Analytical Technologies

Table of Contents

Introduction Specialty HPLC Detectors

Analytical Technologies
Corona Veo Charged Aerosol Detector
Glyceride Analysis
Charged Aerosol Detection provides near universal detection indepen-
Triglyceride Analysis dent of chemical structure for non- or semi-volatile analytes with HPLC
and UHPLC. A Thermo Scientific™ Dionex™ Corona™ Veo™ Charged
Poly-Unsaturated Fatty Acids Aerosol detector is ideally suited as a primary detector for any laboratory,
while providing complementary data to UV or MS methods. No other
Antioxidant Additives
LC detector available today can match the performance of a Corona Veo
References detector.
• High sensitivity – single-digit nanogram on column
• Consistent response – independent of chemical structure
• Wide dynamic range – to four orders of magnitude or greater
• Simple to use – easy to integrate with any HPLC/UHPLC system
The Corona Veo detector gives the simplicity, reproducibility and perfor- Corona Veo Charged Aerosol Detector
mance required for a full range of applications from basic research to
manufacturing QC/QA. With charged aerosol detection you get predict-
able responses to measure analytes in direct proportion to their relative
amounts for quantitation without actual standards.
This detector offers the flexibility to use reversed-phase gradients, as well
as normal phase and HILIC modes of separation on any LC system. And,
in many cases eliminates the need for derivatization or sample pre-treat-
ment to provide real dilute-and-shoot simplicity.

Learn more at www.thermoscientific.com/veo

 Analytical Technologies

Table of Contents

Introduction Specialty HPLC Detectors

Analytical Technologies
Ultimate 3000 Electrochemical Detector Features include:
Glyceride Analysis
Electrochemical detection delivers high sensitivity for neurotransmitter • Detection Modes – choose from DC and PAD for optimum analyte
Triglyceride Analysis analysis, simplicity and robustness for pharmaceutical or clinical diagnos- response
tics, and the selectivity for the characterization of complex samples such • Choice of sensors – both coulometric and amperometric sensors to
Poly-Unsaturated Fatty Acids as natural products, biological tissues and fluids. For today’s researcher, meet the demands of any application
there is a continuing need for detecting vanishingly small quantities of
Antioxidant Additives • UHPLC compatibility – ultralow peak dispersion and high data acquisi-
analyte and often in complex samples. Because electrochemical detection
tion rates for conventional or fast, high resolution chromatography
References measures only compounds that can undergo oxidation or reduction it is
both highly sensitive and very selective. • Modularity – easily expandable to multiple independent sensors for
unrivaled flexibility
The Thermo Scientific Dionex UltiMate 3000 Electrochemical Detec-
tor, designed by the pioneers of coulometric electrochemical detection, • Autoranging – simultaneously measure both low and high levels of
delivers state-of-the-art sensor technologies complete with an entire range analytes without losing data
of high performance and ultra-high performance LC systems optimized • SmartChip™ technology – easy operation with automatic sensor
for electrochemical detection. The UltiMate 3000 ECD-3000RS takes recognition, event logging and electrode protection
electrochemical detection to the next level with UHPLC compatibility, total
system integration, and selection of detection mode, all with unprecedented
operational simplicity.

UltiMate 3000 Electrochemical Detector

Learn more at www.thermoscientific.com/ECDetection

 Analytical Technologies

Table of Contents

Introduction Specialty HPLC Detectors

Analytical Technologies
CoulArray Multi-electrode Array Detector
Glyceride Analysis
The Thermo Scientific™ Dionex™ CoulArray™ Multi-electrode Array de-
Triglyceride Analysis tector is the only practical multi-channel electrochemical detection system
that allows you to measure multiple analytes simultaneously, including
Poly-Unsaturated Fatty Acids those that are chromatographically unresolved. The CoulArray detec-
tor delivers the widest dynamic range of any available electrochemical
Antioxidant Additives
detector with unmatched selectivity for detection of trace components in
References complex matrixes, even when used with aggressive gradients.
• Measures analytes from femtomole to micromole levels
• Greatly simplify sample preparation and eliminate interferences
• Simultaneously analyze multiple analytes in very complex samples
• Easily produce qualitative information for compound identification
Multiple system configurations offer 4, 8, 12, or 16 channels that can be
CoulArray Multi-electrode Array Detector
upgraded anytime. The unique data acquisition and processing software
uses automatic signal ranging and a unique patented baseline correction
algorithms to provide identification and quantitation of single or multiple
analytes and powerful 3D data for quick sample fingerprint confirmation
with integration to pattern recognition platforms.
With the power of coulometric array technology, the CoulArray detector
can give you the qualitative data of a optical PDA with 1,000 fold greater
sensitivity to profile the characteristic qualities of products, determine
integrity, identify adulteration and even evaluate competitors’ products.

Learn more at www.thermoscientific.com/coularray

 Analytical Technologies

Table of Contents

Introduction Specialty HPLC Detectors

Analytical Technologies
Ultimate 3000 Fluorescence Detector • Unsurpassed reproducibility with active flow cell temperature control for
Glyceride Analysis
The Thermo Scientific Dionex UltiMate 3000 FLD-3000 is a high-sensitivity stable fluorophore activity independent of changes in ambient temperature
Triglyceride Analysis fluorescence detector series for UltiMate 3000 HPLC systems. It is available in • Long-life xenon flash lamp for highest sensitivity and long-term operation
Rapid Separation (RS) and Standard (SD) versions. The optics of the FLD- without the need for frequent lamp changing
Poly-Unsaturated Fatty Acids 3000 series provide maximum stray-light suppression for best detection • Optional second photomultiplier (PMT) for unique Dual-PMT operation,
sensitivity. Operated with the Chromeleon CDS software, the detector provides offering an extended wavelength range up to 900 nm without sacrificing
Antioxidant Additives
automated qualification, various tools for method development, and instrument sensitivity in the standard wavelength range
References wellness monitoring for ease of use, maximum uptime, and the highest degree
• Two-dimensional (2D) or three dimensional (3D) excitation, emission, or
of regulatory compliance.
synchro scans to provide the highest degree of flexibility for method devel-
• Data collection at up to 200 Hz for optimal support of even the fastest opment or routine sample characterization
UHPLC separations (FLD-3400RS)
• Innovative Variable Emission Filter for real-time compound-related sensitivity
• Standard detectors operate at up to 100 Hz data rate for optimum support optimization (FLD-3400RS only)
of 62 MPa (9,000 psi) UltiMate 3000 standard systems
• Large front-panel display for easy monitoring of the detector status
• Lowest limits of detection with a Raman signal-to-noise ratio
• Two flow-cell sizes for easy optimization to application requirements: the
(S/N): > 550 ASTM (> 2100 using dark signal as noise reference)
8 µL flow cell is ideal for trace analysis, and the 2 µL flow cell offers best
peak resolution with narrow-bore HPLC and UHPLC columns

Ultimate 3000 Fluorescence Detector

Learn more at www.thermoscientific.com/liquidchromatography

Table of Contents

Introduction

Analytical Technologies

Glyceride Analysis

Triglyceride Analysis
Analytical Technologies
Poly-Unsaturated Fatty Acids

Antioxidant Additives

References

Ion Chromatography

Thermo Scientific Dionex IC systems have led the

analytical instrument industry for over 30 years with
solutions that represent state-of-the art technological
advancements and patented technologies.
 Analytical Technologies

Table of Contents

Introduction IC and RFIC Systems

Analytical Technologies
Innovative Ion Chromatography Solutions water to deliver unmatched sensitivity. RFIC-ER systems are designed to use
Glyceride Analysis
Our High-Pressure™ Ion Chromatography (HPIC™) systems include the carbonate, carbonate/ bicarbonate, or MSA eluents for isocratic separations.
Triglyceride Analysis Thermo Scientific Dionex ICS-5000+ HPIC system, which is optimized for At the heart of our ion chromatography portfolio is a unique set of column
flexibility, modularity, and ease-of-use, combining the highest chromato- chemistries that provide high selectivities and efficiencies with excellent
Poly-Unsaturated Fatty Acids graphic resolution with convenience. In addition, the Thermo Scientific peak shape and resolution. Thermo Scientific™ Dionex™ IonPac™ chroma-
Dionex ICS-4000 Capillary HPIC system is the world’s first commercially tography columns address a variety of chromatographic separation modes
Antioxidant Additives including ion exchange, ion exclusion, reversed-phase ion pairing, and ion
available dedicated capillary high-pressure Reagent-Free™ (RFIC™) IC
References system. The Dionex ICS-4000 system is always ready for the next analysis, suppression. Our column chemistries are designed to solve specific appli-
delivering high-pressure IC on demand. cations, and we offer a variety of selectivities and capacities for simple and
Reagent-Free IC systems eliminate daily tasks of eluent and regenerant complex samples. Additionally, our Dionex IonPac column line is available in
preparation in turn saving time, preventing errors, and increasing conve- standard bore, microbore and capillary formats for the ultimate application
nience. RFIC-EG systems use electrolytic technologies to generate eluent flexibility.
on demand from deionized water, and to suppress the eluent back to pure

Thermo Scientific Dionex IC instrument family

Learn more at www.thermoscientific.com/IC

Table of Contents

Introduction

Analytical Technologies

Glyceride Analysis

Triglyceride Analysis
Analytical Technologies
Poly-Unsaturated Fatty Acids

Antioxidant Additives

References

Mass Spectrometry

Thermo Fisher Scientific provides advanced integrated

IC/MS and LC/MS solutions with superior ease-of-use
and modest price and space requirements. UltiMate
3000 System Wellness technology and automatic MS
calibration allow continuous operation with minimal
maintenance. The Dionex ion chromatography family
automatically removes mobile phase ions for effort-free
transition to MS detection.
 Analytical Technologies

Table of Contents

Introduction Mass Spectrometry Instruments

Analytical Technologies
Single-Point Control and Automation • Chromeleon CDS software for single-point method setup, instrument
Glyceride Analysis
Thermo Fisher Scientific provides advanced integrated IC/MS and control, and data management compatible with existing IC and LC
LC/MS solutions with superior ease-of-use and modest price and space methods
Triglyceride Analysis
requirements. UltiMate 3000 System Wellness technology and automatic • The complete system includes the MSQ Plus mass spectrometer, PC
Poly-Unsaturated Fatty Acids MS calibration allow continuous operation with minimal maintenance. The data system, electrospray ionization (ESI) and atmospheric pressure
Dionex ion chromatography family automatically remove mobile phase chemical ionization (APCI) probe inlets, and vaccum system
Antioxidant Additives
ions for effort-free transition to MS detection. Now, you no longer need two software packages to operate your
References • Thermo Scientific MSQ Plus mass spectrometer, the smallest and
™ ™ LC/MS system. Chromeleon CDS software provides single-software
most sensitive single quadrupole on the market for LC and IC method setup and instrument control; powerful UV, conductivity, and MS
• Self-cleaning ion source for low maintenance operation data analysis; and fully integrated reporting.

MSQ Plus Mass Spectrometer

Learn more at www.thermoscientific.com/MS

Table of Contents

Introduction

Analytical Technologies

Glyceride Analysis

Triglyceride Analysis
Analytical Technologies
Poly-Unsaturated Fatty Acids

Antioxidant Additives

References

Chromatography Data Systems

Tackle chromatography management challenges with

the world’s most complete chromatography software.
Whether your needs are simple or complex or your
scope is a single instrument, a global enterprise, or
anything in between – the combination of Chromeleon
CDS’ scalable architecture and unparalleled ease-
of use, makes your job easy and enjoyable with one
Chromatography Data System for the entire lab.
 Analytical Technologies

Table of Contents

Introduction Chromeleon CDS Software

Analytical Technologies
The Fastest Way from Samples to Results • Enjoy a modern, intuitive user interface designed around the principle
Glyceride Analysis
The 7.2 release of Chromeleon Chromatography Data System software of operational simplicity
Triglyceride Analysis is the first CDS that combines separation (GC/IC/LC) and Mass Spec- • Streamline laboratory processes and eliminate errors with eWork-
trometry (MS) in an enterprise (client/server) environment. By extending flows™, which enable anyone to perform a complete analysis perfectly
Poly-Unsaturated Fatty Acids Chromeleon 7.2 CDS beyond chromatography into MS, lab technicians with just a few clicks
can now streamline their chromatography and MS quantitation workflows • Access your instruments, data, and eWorkflows instantly in the Chro-
Antioxidant Additives
with a single software package. MS support in Chromeleon 7.2 CDS is meleon Console
References focused on routine and quantitative workflows, which provides access to
• Locate and collate results quickly and easily using powerful built-in
rich quantitative data processing and automation capabilities — ultimately
database query features
boosting your overall lab productivity and increasing the quality of your
analytical results. • Interpret multiple chromatograms at a glance using MiniPlots
• Find everything you need to view, analyze, and report data in the
Chromatography Studio
• Accelerate analyses and learn more from your data through dynamic,
interactive displays
• Deliver customized reports using the built-in Excel compatible spead-
sheet

Learn more at www.thermoscientific.com/Chromeleon

Table of Contents

Introduction

Analytical Technologies

Glyceride Analysis

Triglyceride Analysis
Analytical Technologies
Poly-Unsaturated Fatty Acids

Antioxidant Additives

References

Process Analytical Systems

Thermo Scientific Dionex process analytical systems

provide timely results by moving chromatography-based
measurements on-line.
 Analytical Technologies

Table of Contents

Introduction Process Analytical Systems and Software

Analytical Technologies
Improved Process Monitoring with On-line • Characterize your samples completely with multicomponent analysis
Glyceride Analysis Chromatography IC and LC Systems • Reduce sample collection time and resources with automated multi-
Triglyceride Analysis Information from the Thermo Scientific Dionex Integral process analyzer point sampling
can help reduce process variability, improve efficiency, and reduce down- • Improve your process control with more timely results
Poly-Unsaturated Fatty Acids time. These systems provide comprehensive, precise, accurate informa- • See more analytes with unique detection capabilities
tion faster than is possible with laboratory-based results. From the lab to
Antioxidant Additives • The Thermo Scientific Integral Migration Path approach lets you
the factory floor, your plant’s performance will benefit from the information
provided by on-line LC. choose the systems that best meets your needs
References

Integral process analytzer

Learn more at www.thermoscientific.com/chromatography

Table of Contents

Introduction

Analytical Technologies

Glyceride Analysis

Triglyceride Analysis
Analytical Technologies
Poly-Unsaturated Fatty Acids

Antioxidant Additives

References

Automated Sample Preparation

Solvent extractions that normally require labor-intensive

steps are automated or performed in minutes, with
reduced solvent consumption and reduced sample
handling using the Thermo Scientific™ Dionex™ ASE™
Accelerated Solvent Extractor system or Thermo
Scientific™ Dionex™ AutoTrace™ 280 Solid-Phase
Extraction instrument.
 Analytical Technologies

Table of Contents

Introduction Accelerated Solvent Extractor System

Analytical Technologies
Complete Extractions in Less Time Using ing process that makes it inert to chemical attack by acids and bases at
Glyceride Analysis Less Solvent elevated temperatures.
Triglyceride Analysis Thermo Scientific Dionex ASE systems extract of solid and semisolid Dionex ASE systems are dramatically faster than Soxhlet, sonication, and
samples using common solvents at elevated temperature and pressure. other extraction methods, and require significantly less solvent and labor.
Poly-Unsaturated Fatty Acids The Dionex ASE 150 and 350 systems feature pH-hardened pathways Accelerated solvent extraction methods are accepted and established
with Dionium™ components to support extraction of acidic or alkaline in the environmental, pharmaceutical, foods, polymers and consumer
Antioxidant Additives product industries. Accelerated solvent extraction methods are accepted
matrices, and combine pretreatment, solvent extraction, and cleanup into
References one step. Dionium is zirconium that has undergone a proprietary harden- and used by government agencies worldwide.

Dionex ASE 150/350 and Dionex AutoTrace 280 SPE instruments

Learn more at www.thermoscientific.com/samplepreparation

Table of Contents

Introduction

Analytical Technologies

Glyceride Analysis
Fats, Oils, and Other Lipids
Triglyceride Analysis

Poly-Unsaturated Fatty Acids

Antioxidant Additives

References

Glyceride Analysis

Glycerides, more correctly known as acylglycerols,

are esters formed from glycerol and fatty acids. As
Red palm fruit glycerol has three hydroxyl functional groups, it can be
esterified with one, two, or three fatty acids forming
monoglycerides, diglycerides, and triglycerides,
respectively. Animal fats and vegetable oils contain
mostly triglycerides, but are degraded by enzymes
called lipases into mono and diglycerides and free
fatty acids.
 Fats, Oils, and Other Lipids

Table of Contents

Introduction Glyceride Analysis

Analytical Technologies
Red Palm Oil
Glyceride Analysis
Column: Thermo Scientific™ Accucore™ C18, 150 × 3 mm, 2.6 µm
Triglyceride Analysis Column Temp.: 40 °C
Injection Volume: 10 µL
Mobile Phase: A. Methanol/water/acetic acid (400:600:4)
Poly-Unsaturated Fatty Acids B. Tetrahydrofuran/Acetonitrile (50:950)
C. Acetone / Acetonitrile (900:100)
Antioxidant Additives Gradient: Time (mL/min) %A %B %C
-10 1.0 90 10 0
0 1.0 90 10 0
References 30 1.5 15 85 0
75 1.5 7 85 8
82 1.5 0 0 100
85 1.0 90 10 0
90 1.0 90 10 0
Samples: 100 µL + 900 µL methanol/chloroform (1:1)

300 Triglycerides

pA Diglycerides

Fatty Acids
Did You Know?
The highly saturated nature of palm oil renders it solid at room temperature,
making it a cheap substitute for butter in uses where solid fat is required, such
0 as the making of pastry dough and baked goods. It is less of a health-hazard
-20 than the alternative substitute of partially hydrogenated trans fat.
0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75
Minutes
Figure 3-1. Separation of fatty acids, diglycerides and triglycerides in red palm oil extracts by
HPLC-Charged Aerosol Detection.

Download Application Note 1039: Simultaneous Analysis of Glycerides (Mono-, Di-, and Triglyerides) and Free Fatty Acids in Palm Oil
 Fats, Oils, and Other Lipids

Table of Contents

Introduction Glyceride Analysis

Analytical Technologies
Glyceride Patterns in Oils
Glyceride Analysis
Column: Accucore C18, Column: Accucore C18, 150 × 3.0 mm,
150 × 3.0 mm, 2.6 µm, 50 °C
Triglyceride Analysis 2.6 µm, 50 °C Flow: 0.8–1.2 mL/min
Flow: 0.8–1.2 mL/min Injection Volume: 5 µL
Poly-Unsaturated Fatty Acids Injection Volume: 5 µL Mobile Phase: A. Acetonitrile
180 B. 2-Propanol
Corn Oil Gradient: Time (mL/min) %A %B
Antioxidant Additives -6.0 1.0 100 0
-0.2 1.2 100 0
References -0.1 0.8 100 0
0.0 0.8 100 0
0.2 1.2 100 0
2.0 1.2 90 10
pA
25.0 1.2 60 40
30.0 1.0 40 60
30.0 1.0 100 0
Samples: 100 µL + 900 µL methanol/
chloroform (1:1)

0
-20
200
Olive Oil

pA

0
-20
0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30
Minutes
Figure 3-2. Glyceride patterns in corn oil and olive oil using HPLC with Charged Aerosol Detection.

Download the Poster Note: Characterization of Used Cooking Oils by HPLC-Charged Aerosol Detection
Download the Poster Note: An Improved Global Method for the Quantitation and Characterization of Lipids by HPLC-Charged Aerosol Detection
 Fats, Oils, and Other Lipids

Table of Contents

Introduction Triglyceride Analysis

Analytical Technologies
Separation of triglycerides in different cooking oils and improved 50 Longer Chain Lipids
Glyceride Analysis resolution using the Accucore C30 column.
A Accucore C30
B C
Triglyceride Analysis 2
System: UltiMate 3000 RS system with LPG-3600-RS dual-ternary pump, 2 D
1 34 1
WPS-3000 RS thermostatted split-loop autosampler, 1 3 2
5 3 1 2
Poly-Unsaturated Fatty Acids and TCC-3000 RS column thermostat, with an aerosol detector mV
Column 1: Accucore C30 2.6 µm, 100 mm × 3 mm
Column 2: Accucore C18 2.6 µm, 100 mm × 3 mm
Antioxidant Additives Accucore C18
Column 3: Accucore C30 2.6 µm, 100 mm × 4.6 mm
Temperature: 30 °C A
B
References Flow: 1.25 mL/min (4.6 mm bore) or 0.50 mL/min (3 mm bore) 2 C
Injection Volume: 5 µL x 1 mg/mL (4.6 mm bore) or 1 µL × 10 mg/mL (3 mm bore) 1 D
1
1 3 2 2 1 2
Mobile Phase: A. Acetonitrile 0
B. Isopropanol
2 4 6 8 10 12 14 16 18
C. 7.7 g/L ammonium acetate Minutes
+ 2.0 g/L acetic acid, pH 5.2
Gradient: Isocratic: 25% A, 70% B, 5% C Figure 3-4. Comparison chromatograms showing the difference in selectivity achieved using an
200 Accucore C30 HPLC column compared to an Accucore C18 HPLC column for the analysis of
peanut oil. See Figure 3-3 for conditions. The Accucore C30 column improved analyte resolution
Canola within peaks clusters A through D.

Sesame
pA
Olive

0 Peanut

0 2 4 6 8 10 12 14 16 18 20
Minutes
Figure 3-3. Comparison of chromatograms showing the difference in constituents amongst four
types of cooking oil using an Accucore C30 HPLC column and charged aerosol detection.

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Table of Contents

Introduction Triglyceride Analysis

Analytical Technologies
Triglyceride Analysis: Milk
Glyceride Analysis Did You Know?
Column: Solid-core C8, 150 × 4.6 mm, 2.7 µm, 40°C • Before milking machines were invented in 1894, farmers could only milk about
Triglyceride Analysis Flow: 0.8 mL/min
6 cows per hour. Now it takes less than 5 minutes to milk a cow using a milking
Injection Volume: 10 µL
Mobile Phase: A. Methanol/Water/Acetic Acid (750:250:4) machine.
Poly-Unsaturated Fatty Acids B. Acetonitrile/Methanol/THF/Acetic Acid (500:375:125:4)
Detector: Charged Aerosol
• The first cow in America arrived in the Jamestown colony in 1611. Until the
Antioxidant Additives 1850s, nearly every family had its own cow.
• Cows drink about 35 gallons of water a day – about the same amount as a
References bathtub full of water.

Figure 3-5. Whole milk was extracted through a modified Association of Official Analytical Chem-
ists (AOAC) method, using ammonium hydroxide, hexane, and ether.
 Fats, Oils, and Other Lipids

Table of Contents

Introduction Triglyceride Speciation

Analytical Technologies
Castor oil is a natural oil that, in its native state, has many uses ranging HPLC System: UltiMate 3000 HPLC system Detector: Corona ultra
Column: Acclaim RSLC 120 C8, Nebulizer Heater: 20 °C
Glyceride Analysis from personal care (laxative, cosmetics, topicals), through chemical (raw 2.2 µm 150 × 2.1 mm Filter: High
materials), to industrial (lubricants, hydraulic fluids, dielectric fluids, textiles, Flow: 0.7 mL/min Sample Solvent: Alcohol, denatured
Triglyceride Analysis Column Temp.: 50 °C Peaks: 7. RRR
paints, coatings). In the food industry, food grade castor oil is used in food Injection Volume: 5 µL at 20 °C 9. RRLs
Poly-Unsaturated Fatty Acids additives, flavorings, chocolates, as a mold inhibitor, and in packaging. Mobile Phase A A. Methanol / Water (900 : 100) 13. RRLn
B. Isopropanol 14. RRL
The composition of castor oil is unique in that it contains a triglyceride, Gradient: Time (min) %B 16. RRO
Antioxidant Additives 0 0 18. RRS
RRR, composed of the omega-9 unsaturated fatty acid, ricinoleic acid (R) 3 15

References
(12-hydroxy-9-cis-octadecenoic acid). Seventy percent of the castor oil 20 20
25 90
is the triglyceride RRR, and the remaining triglycerides contain primarily 28 100
oleic and linoleic acids. 28 0
33 0
24.6
7
14
16

10 11
4 12 15 18
9
6 13
1
8 19 20
23 5 17
Figure 3-6. Structure of triricinoleate. 0

0 1 2 3 4 5 6 7 8 9 10 11 12 13

Figure 3-7. Chromatogram of castor oil A at 500 ng on column.

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Table of Contents

Introduction

Analytical Technologies

Glyceride Analysis
Fats, Oils, and Other Lipids
Triglyceride Analysis

Poly-Unsaturated Fatty Acids

Antioxidant Additives

References

Poly-Unsaturated Fatty Acids

Numerous polyunsaturated fatty acids (PUFAs) are found

in foods. They vary in chain length, position and number
of double bonds, and whether such double bonds
are in a cis- or trans-configuration. Some of the more
important PUFAs include the omega fatty acids such as
the omega-3 (α-linolenic acid (ALA), eicosapentaenoic
Eicosapentaenoic acid
structure acid (EPA) and docosahexaenoic acid (DHA), omega-6
(linoleic acid and arachidonic acid), omega-7 (palmitoleic
acid and vaccenic acid), and omega-9 (oleic acid and
erucic acid) fatty acids. Although omega-3 fatty acids
and omega-6 fatty acids are essential components of the
diet their purported health benefits (or detractions) require
further scientific evaluation.
 Fats, Oils, and Other Lipids

Table of Contents

Introduction Poly-Unsaturated Fatty Acids

Analytical Technologies
System: UltiMate 3000 RSLC Dual Gradient Peaks:
Column: Acclaim C30 3 µm, 250 × 3 mm 4. Stearadonic Acid 15. Arachidonic acid 15. Adrenic acid
Glyceride Analysis Flow: 1 mL/min 8. Eicosapentanoic acid 17. Linoleic acid 27. Oleic acid
Temperature: 30 °C 9. a-Linolenic acid 18. Docosapentanoic acid 30. Eicosadienoic acid
Triglyceride Analysis Inverse Gradient 10. g-Linoleic acid 19. 9E, 14Z- conjugated 36. Erucic acid
Pump: 1 mL/min 14. Docosahexanoic acid linoleic acid
Mobile Phase: A. Water/formic acid/ 100 22. Eicosatrienoic acid
Poly-Unsaturated Fatty Acids mobile phase B (900:3.6:100);
B. Acetone/acetonitrile/tetrahydrofuran/
Antioxidant Additives formic acid (675:225:100:4)
Detector: Charged Aerosol

References Peaks pA
8
1. Stearadonic Acid 8. Docosapentanoic acid 26
2. Eicosapentanoic acid 9. 9E, 14Z- conjugated linoleic acid 14 25 27
50 9
3. a-Linolenic acid 10. Eicosatrienoic acid 11 32
4. g-Linoleic acid 11. Adrenic acid 6 16 19 29
13 17 23 30 33 37
5. Docosahexanoic acid 12. Oleic acid 4 18 20 22 28 31 35
1 23 5 7 10 12 15 21 24 34 36 38
6. Arachidonic acid 13. Eicosadienoic acid
7. Linoleic acid 14. Erucic acid -5
6.0 7.5 8.8 10.0 11.3 12.5 13.8 15.0 16.3 17.5 18.8 20.0 21.3 23.0
pA 14 Minutes
9 Figure 3-9. HPLC chromatogram of 20 µL hydrolyzed fish oil with addition of 200 µL isopropanol
11 13
to aid solubility. A total of 38 peaks were detected including all 14 standards. For conditions see
5 8 10 12
3
6 7
Figure 3-8.
1 4
2
0
-5
6.0 7.5 8.8 10.0 11.3 12.5 13.8 15.0 16.3 17.5 18.8 20.0 21.3 23.0
Minutes
Figure 3-8. Separation of PUFA standards.

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 Fats, Oils, and Other Lipids

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Introduction Poly-Unsaturated Fatty Acids

Analytical Technologies
148 Peaks: Table 3-1. Percent compositions of HPLC-Charged Aerosol Detection analysis of hydrolyzed samples.
1. α-Linolenic acid
Glyceride Analysis 5. Linoleic acid Sample Omega-3 (%) Omega-6 (%) Omega-9 (%) 3:6 Ratio
15
6. Eicosatrienoic acid
8. Adrenic acid Fish Oil 92.0 3.2 4.5 29.0
Triglyceride Analysis
9. Oleic acid Fish, Flax, Borage 81.0 13.0 5.9 6.1
pA 10. Eicosadienoic acid Supplement
Poly-Unsaturated Fatty Acids 15. Erucic acid
Other peaks are unidentified
Flax Oil 59.0 22.0 19.0 2.7
Antioxidant Additives 1213 Beef, Grass-fed 23.0 24.0 52.0 0.95
5 9
1
14 16
23 4 6 7 8 10 11 Avocado Oil 15.0 22.0 64.0 0.68
References 0
Chicken, Pastured 25.0 40.0 35.0 0.62
12.9 14.0 15.0 16.0 17.0 18.0 19.0 20.0 21.0 22.0 23.0 24.1
Minutes Mustard Oil 14.0 23.0 63.0 0.62
Figure 3-10. HPLC chromatogram of hydrolyzed mustard oil using a C30 150 x 4.5 mm, 5 µm column. Canola Oil 13.2 33.2 53.6 0.39
Olive Oil 4.8 13.0 82.0 0.37
Walnut Oil 12.0 75.0 13.0 0.16
160 Peaks Castor Oil 4.2 62.0 34.0 0.067
3
3. Rincinoleic acid
7. Linolenic acid Safflower Oil 0.71 17.0 82.0 0.041
8. Linoleic acid Sesame Oil 1.5 61.0 38.0 0.024
9. Adrenic acid
10. Oleic acid Corn Oil 1.6 72.0 26.0 0.022
pA 11. Eicosadienoic acid

Other peaks are unidentified

1314
1 8 10 12
2 4 5 6 7 9 11

–20
6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Minutes
Figure 3-11. HPLC chromatogram of hydrolyzed castor oil with ricinoleate-free fatty acid at
8.54 min.

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Table of Contents

Introduction

Analytical Technologies

Glyceride Analysis
Fats, Oils, and Other Lipids
Triglyceride Analysis

Poly-Unsaturated Fatty Acids

Antioxidant Additives

References

Antioxidant Additives to
Prevent Rancidity
Polyunsaturated fatty acids are unstable, undergoing
an uncontrolled chain reaction called lipid
peroxidation, where the result is rancidity.
The bad odor associated with rancid oils and fats
comes from the formation of oxidation products
Omega-3 oil capsules including potentially toxic aldehydes (e.g.,
malondialdehyde and 4-hydroxynonenal).
Antioxidants prevent lipid peroxidation and are added
to foods and food packaging in order to prevent
rancidity and extend product shelf life.
 Fats, Oils, and Other Lipids

Table of Contents

Introduction Antioxidant Additives

Analytical Technologies
CO2CH2CH2CH3 OH
COCH2CH2CH3
Glyceride Analysis
OH (CH3)3C C(CH3)3
Triglyceride Analysis

Poly-Unsaturated Fatty Acids HO OH HO

Antioxidant Additives OH OH CH3

Propyl gallate (PG) 2,4,5-Trihydroxy-butyrophenone (THBP) 3,5-Di-tert-butyl-4-hydroxytoluene (BHT)
References
OH C(CH3)3 CO2(CH2)11CH3
C(CH3)3

C(CH3)3

HO OH

OH OH OH
tert-Butylhydroquinone (TBHQ) 2 and 3-tert-Butyl-4-hydroxyanisole (BHA) Lauryl gallate (dodecyl gallate)

OH CO2(CH2)7CH3
CH3
(CH3)3C C(CH3)3

HO CH2CH
HO OH

HO 2 OH
CH2OH

Nordihydroguaiaretic acid (NDGA) Octyl gallate

2,6-Di-tert-4-hydroxymethylphenol (lonox 100)

Figure 3-12. Chemical structures of various antioxidant additives.

 Fats, Oils, and Other Lipids

Table of Contents

Introduction Antioxidant Additives

Analytical Technologies
The gradient analytical system for this application consisted of two pumps,
Glyceride Analysis an autosampler, a thermostatic chamber, an 8-channel CoulArray detector
and UV detector. In addition, a guard cell was placed after the mixer to
Triglyceride Analysis
oxidize contaminants in the mobile phase that co-eluted with BHT.
Poly-Unsaturated Fatty Acids
Column: C18 (150 × 4.6 mm, 5 µm)
Antioxidant Additives Flow: 1.75 mL/min
Temperature: 40 °C
Injection Volume: 20 µL
References Mobile Phase A: Water containing 25 mM sodium acetate and
25 mM citric acid-methanol; 95:5 (v/v).
Mobile Phase B: Water containing 25 mM sodium acetate and
25 mM citric acid-methanol-ACN; 20:40:40 (v/v/v).
Gradient Conditions: Initial conditions of 25% B with linear increase to 100% B
over 12 minutes; hold at 100% B for 8 minutes;
return to initial conditions of 25% B; and hold for 10 minutes
Electrochemical Detector: Model 5600A, CoulArray Figure 3-14. Chromatogram of an extracted lard sample.
Applied Potentials: -50, 0, 70, 250, 375, 500, 675, 825 mV (vs. Pd).
UV Detector: Model 520 or 522.
Wavelength: 280 nm (0.01 AUFS).

Figure 3-13. Gradient HPLC-CoulArray electrochemical detector chromatogram showing resolution

of nine commonly used antioxidant additives.

Download Application Brief 159: Direct Determination of Phenolic Antioxidants in Food Oils and Hand Creams
Table of Contents

Introduction

Analytical Technologies

Glyceride Analysis

Triglyceride Analysis References

Poly-Unsaturated Fatty Acids

Antioxidant Additives

References

Technical Collateral and

Peer Reviewed Journals
Here you’ll find a multitude of references using our
HPLC, ion chromatography and sample preparation
solutions.
Table of Contents

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Analytical Technologies

Glyceride Analysis

Triglyceride Analysis References

Poly-Unsaturated Fatty Acids

Antioxidant Additives

References

HPLC and UHPLC References

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Analytical Technologies HPLC and UHPLC Methods
Glyceride Analysis Carbohydrates
Triglyceride Analysis
Title Authors Publication Publication Date
Poly-Unsaturated Fatty Acids Carbohydrate and oligosaccharide analysis with a universal HPLC detector Asa, D. American Laboratory 38, 16. 2006
Determination of levoglucosan in atmospheric aerosols using high performance liquid J. Chromatogr., A. 1109 (2),
Dixon, R. W.; Baltzell, G. 2006 Mar 24
Antioxidant Additives chromatography with aerosol charge detection 214–221
Composition of structural carbohydrates in biomass: Precision of a liquid
References Godin, B.; Agneessens, R.; Gerin, P. A.;
chromatography method using a neutral detergent extraction and a charged aerosol Talanta 85 (4), 2014–2026 2011 Sep 30
Delcarte, J.
detector
Selectivity issues in targeted metabolomics: Separation of phosphorylated Hinterwirth, H.; Lämmerhofer, M.; J. Sep. Sci. 33 (21), 3273–
carbohydrate isomers by mixed-mode hydrophilic interaction/weak anion exchange Preinerstorfer, B.; Gargano, A.; Reischl, R.; 2010 Nov
3282
chromatography Bicker, W.; Trapp, O.; Brecker, L.; Lindner, W.
Investigation of polar organic solvents compatible with Corona charged aerosol Hutchinson, J. P.; Remenyi, T.; Nesterenko, P.; Anal. Chim. Acta. 750,
detection and their use for the determination of sugars by hydrophilic interaction liquid Farrell, W.; Groeber, E.; Szucs, R.; Dicinoski, G.; 2012 Oct 31
199–206
chromatography Haddad, P. R.
Characterization of an endoglucanase belonging to a new subfamily of glycoside Appl. Environ. Microbiol. 74
Igarashi, K.; Ishida, T.; Hori, C.; Samejima, M. 2008 Sep
hydrolase family 45 of the basidiomycete Phanerochaete chrysosporium (18), 5628–5634
Direct detection method of oligosaccharides by high-performance liquid Biomed. Chromatogr. 21 (4),
Inagaki, S.; Min, J. Z.; Toyo’oka, T. 2007 Apr
chromatography with charged aerosol detection 338–342
Kim, J. H.; Lim, B. C.; Yeom, S. J.; Kim, Y. S.;
Differential selectivity of the Escherichia coli cell membrane shifts the equilibrium for Appl. Environ. Microbiol. 74 (8),
Kim, H. J.; Lee, J. K.; Lee, S. H.; Kim, S. W.; 2008 Apr
the enzyme-catalyzed isomerization of galactose to tagatose 2307–2313
Oh, D. K.
Elution strategies for reversed-phase high-performance liquid chromatography J. Chromatogr., A. 1311,
Lie, A.; Pedersen, L. H. 2013 Oct 11
analysis of sucrose alkanoate regioisomers with charged aerosol detection 127–133
Design of experiments and multivariate analysis for evaluation of reversed-phase
J. Chromatogr., A. 1281,
high-performance liquid chromatography with charged aerosol detection of sucrose Lie, A.; Wimmer, R.; Pedersen, L. H. 2013 Mar 15
67–72
caprate regioisomers
Solvent effects on the retention of oligosaccharides in porous graphitic carbon liquid Melmer, M.; Stangler, T.; Premstaller, A.; J. Chromatogr., A 1217 (39)
2010 Sep 24
chromatography Lindner, W. 6092–6096
Biosci., Biotechnol., Biochem.
Practical preparation of lacto-N-biose I, a candidate for the bifidus factor in human milk Nishimoto, M.; Kitaoka, M. 2007 Aug
71 (8), 2101–2104
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Analytical Technologies HPLC and UHPLC Methods
Glyceride Analysis Carbohydrates
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Title Authors Publication Publication Date
Poly-Unsaturated Fatty Acids Cellotriose and cellotetraose as inducers of the genes encoding cellobiohydrolases in Appl. Environ. Microbiol. 76
Suzuki, H.; Igarashi, K.; Samejima, M. 2010 Sep
the basidiomycete Phanerochaete chrysosporium (18), 6164–6170
Antioxidant Additives Wada, J.; Honda, Y.; Nagae, M.; Kato, R.;
1,2-alpha-l-Fucosynthase: A glycosynthase derived from an inverting FEBS Lett. 582 (27),
Wakatsuki, S.; Katayama, T.; Taniguchi, H.; 2008 Nov 12
alpha-glycosidase with an unusual reaction mechanism 3739–3743
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Westereng, B.; Agger, J. W.; Horn, S. J.;
Efficient separation of oxidized cello-oligosaccharides generated by cellulose J. Chromatogr., A. 1271 (1),
Vaaje-Kolstad, G.; Aachmann, F. L.; 2013 Jan 4
degrading lytic polysaccharide monooxygenases 144–152
Stenstrøm, Y. H.; Eijsink, V. G.
Xiao, J. Z.; Takahashi, S.; Nishimoto, M.;
Distribution of in vitro fermentation ability of lacto-N-Biose I, a major building block of Appl. Environ. Microbiol. 76 (1),
Odamaki, T.; Yaeshima, T.; Iwatsuki, K.; 2010 Jan
human milk oligosaccharides, in bifidobacterial strains 54–59
Kitaoka, M.
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Analytical Technologies HPLC and UHPLC Methods
Glyceride Analysis Food, Nutrition, Natural Products, and Supplements
Triglyceride Analysis
Title Authors Publication Publication Date
Poly-Unsaturated Fatty Acids Characterization of phenolic compounds in strawberry (Fragaria x ananassa)
J. Agric. Food Chem. 55 (11),
fruits by different HPLC detectors and contribution of individual compounds to Aaby, K.; Ekeberg, D.; Skrede, G. 2007 May 30
4395–4406
Antioxidant Additives total antioxidant capacity
Analysis of flavonoids and other phenolic compounds using high-performance
J. Agric. Food Chem. 52 (15),
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Aqueous extract of Astragali Radix induces human natriuresis through Ai, P.; Yong, G.; Dingkun, G.; Qiuyu, Z.; J. Ethnopharmacol. 116 (13),
2008 Mar 28
enhancement of renal response to atrial natriuretic peptide Kaiyuan, Z.; Shanyan, L. 413–421
Antioxidant, α-amylase inhibitory and oxidative DNA damage protective property Akhter, F.; Hashim, A.; Khan, M. S.; Ahmad, S.;
S. Afr. J. Bot. 88, 265–272 2013 Sep
of Boerhaavia diffusa (Linn.) root Iqbal, D.; Srivastava, A. K.; Siddiqui, M. H.
Ameho, C. K.; Chen, C. Y. O.; Smith, D.;
J. Nutr. Biochem. 19 (7),
Antioxidant activity and metabolite profile of quercetin in vitamin-E-depleted rats. Sánchez-Moreno, C.; Milbury, P. E.; Blumberg, 2008 Jul
p.467–474
J. B.
Evaluation of tolerable levels of dietary quercetin for exerting its antioxidative Food Chem. Toxicol. 48 (4),
Azuma, K.; Ippoushi, K.; Terao, J. 2010 Apr
effect in high cholesterol-fed rats 1117–1122
J. Ginseng Res. 36 (2),
Recent methodology in ginseng analysis Baek, S.; Bae, O.; Park, J. 2012 Apr
119–134
Sensitive determination of saponins in radix et rhizoma notoginseng by charged J. Liq. Chromatogr. Relat.
Bai, C.; Han, S.; Chai, X.; Jiang, Y.; Li, P.; Tu, P. 2010 Aug 27
aerosol detector coupled with HPLC Technol. 32 (2), 242–260
Comprehensive analysis of polyphenols in 55 extra virgin olive oils by HPLC-ECD Bayram, B.; Esatbeyoglu, T.; Schulze, N.; Plant Foods Hum. Nutr. (N. Y.,
2012 Dec
and their correlation with antioxidant activities Ozcelik, B.; Frank, J.; Rimbach, G. NY, U.S.) 67 (4), 326–336
Benavides, G. A.; Squadrito, G. L.; Mills, R. W.;
Proc.Natl. Acad. Sci. U.S.A.
Hydrogen sulfide mediates the vasoactivity of garlic Patel, H. D.; Isbell, T. S.; Patel, R. P.; 2007 Nov
104 (46), 17977–17982
Darley-Usmar, V. M.; Doeller, J. E.; Kraus, D. W.
Analysis of selected stilbenes in Polygonum cuspidatum by HPLC coupled with Benová, B.; Adam, M.; Onderková, K.; Královský, J. Sep. Sci. 31 (13), 2404–
2008 Jul
CoulArray detection J.; Krajícek, M. 2409
Rapid and complete extraction of phenols from olive oil and determination by J. Agric. Food Chem. 48 (11),
Brenes, M.; García, A.; García, P.; Garrido, A. 2000 Nov
means of a coulometric electrode array system 5178–5183
The real nature of the indole alkaloids in Cortinarius infractus: Evaluation of Brondz, I.; Ekeberg, D.; Høiland, K.; Bell, D.;
J. Chromatogr., A 1148 (1), 1–7 2007 Apr 27
artifact formation through solvent extraction method development Annino, A.
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Analytical Technologies HPLC and UHPLC Methods
Glyceride Analysis Food, Nutrition, Natural Products, and Supplements
Triglyceride Analysis
Title Authors Publication Publication Date
Poly-Unsaturated Fatty Acids Chemotaxonomic differentiation between Cortinarius infractus and Cortinarius
subtortus by supercritical fluid chromatography connected to a multi-detection Brondz, I.; Høiland, K. Trends Chromatogr. 4, 79–87 2008
Antioxidant Additives system
Brown, M. J.; Ferruzzi, M. G.; Nguyen, M. L.;
Carotenoid bioavailability is higher from salads ingested with full-fat than with Am. J. Clin. Nutr. 80 (2),
References Cooper, D. A.; Eldridge, A. L.; Schwartz, S. J.; 2004 Aug
fat-reduced salad dressings as measured with electrochemical detection 396–403
White, W. S.
Bugianesi, R.; Catasta, G.; Spigno, P.; D’Uva, A.;
Naringenin from cooked tomato paste is bioavailable in men J. Nutr. 132 (11), 3349–3352 2002 Nov
Maiani, G.
“Dilute-and-shoot” triple parallel mass spectrometry method for analysis of vitamin Anal. Bioanal. Chem. 401 (10),
Byrdwell, W. C. 2011 Dec
D and triacylglycerols in dietary supplements 3317–3334
Human skeletal muscle ascorbate is highly responsive to changes in vitamin C Carr, A. C.; Bozonet, S. M.; Pullar, J. M.; Am. J. Clin. Nutr. 97 (4),
2013 Apr
intake and plasma concentrations Simcock, J. W.; Vissers, M. C. 800–807
Utilization of RP-HPLC fingerprinting analysis for the identification of diterpene Int. J. Res. Phytochem.
Chaturvedula, V.; Prakash, I. 2011 Jun 9
glycosides from Stevia rebaudiana Pharmacol. 1 (2), 88–92
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Acid and alkaline hydrolysis studies of stevioside and rebaudioside A Chaturvedula, V.; Prakash, I. 2011 Oct
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Spectral analysis and chemical studies of the sweet constituent, rebaudioside A Chaturvedula, V.; Prakash, I. 2012 Feb
57–65
Flavonoids from almond skins are bioavailable and act synergistically with Chen, C.; Milbury, P. E.; Lapsley, K.;
J. Nutr. 135 (6), 1366–1373 2005 Jun 1
vitamins C and E to enhance hamster and human LDL resistance to oxidation Blumberg, J. B.
J. Agric. Food Chem. 56 (18),
Photostability of rebaudioside A and stevioside in beverages Clos, J. F.; Dubois, G. E.; Prakash, I. 2008 Sep 24
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CoulArray electrochemical evaluation of tocopherol and tocotrienol isomers in Nat. Prod. Commun. 4 (2),
Colombo, M. L.; Marangon, K.; Bugatti, C. 2009 Feb
barley, oat and spelt grains 251–254
Correa, C. R.; Li, L.; Aldini, G.; Carini, M.;
Composition and stability of phytochemicals in five varieties of black soybeans Food Chem. 123 (4),
Oliver Chen, C. Y.; Chun, H.; Cho, S.; Park, K.; 2010 Dec 15
(Glycine max) 1176–1184
Russell, R. M.; Blumberg, J. B.; Yeum, K.
Effect of UV-B light and different cutting styles on antioxidant enhancement of Du, W.; Avena-Bustillos, R. J.; Breksa, A. P., III.; Food Chem. 134 (4),
2012 Oct 15
commercial fresh-cut carrot products McHugh, T. H. 1862–1869
 References

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Introduction Peer Reviewed Journals:

Analytical Technologies HPLC and UHPLC Methods
Glyceride Analysis Food, Nutrition, Natural Products, and Supplements
Triglyceride Analysis
Title Authors Publication Publication Date
Poly-Unsaturated Fatty Acids Durazzo, A.; Turfani, V.; Azzini, E.; Maiani, G.; Food Chem. 140 (4),
Phenols, lignans and antioxidant properties of legume and sweet chestnut flours 2013 Oct 15
Carcea, M. 666–671
Antioxidant Additives alpha-Lipoic acid in dietary supplements: development and comparison of Durrani, A. I.; Schwartz, H.; Schmid, W.; J. Pharm. Biomed. Anal. 45 (4),
2007 Nov 30
HPLC-CEAD and HPLC-ESI-MS methods Sontag, G. 694–699
References Eom, H. Y.; Park, S. Y.; Kim, M. K.; Suh, J. H.;
Comparison between evaporative light scattering detection and charged aerosol J. Chromatogr., A. 1217 (26),
Yeom, H.; Min, J. W.; Kim, U.; Lee, J.; 2010 Jun 25
detection for the analysis of saikosaponins 4347–4354
Youm, J. R.; Han, S. B.
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Assessment of microcystin purity using charged aerosol detection Edwards, C.; Lawton, L. A. 2010 Aug 6
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Poly-Unsaturated Fatty Acids Henning, S. M.; Niu, Y.; Liu, Y.; Lee, N. H.;
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HPLC in natural product analysis: The detection issue Wolfender, J. L. Planta Med. 75 (07), 719–734 2009 Jun
Simultaneous determination of isoflavones and bisphenol A in rat serum by high- Yasuda, S.; Wu, P. S.; Hattori, E.; Tachibana, H.; Biosci., Biotechnol., Biochem.
2004 Jan
performance liquid chromatography coupled with coulometric array detection Yamada, K. 68 (1), 51–58
Impurities from polypropylene microcentrifuge tubes as a potential source of
Clin. Chem. Lab. Med. 42 (4),
interference in simultaneous analysis of multiple lipid-soluble antioxidants by Yen, H. C.; Hsu, Y. T. 2004 Apr
390–395
HPLC with electrochemical detection
 References

Table of Contents

Introduction Peer Reviewed Journals:

Analytical Technologies HPLC and UHPLC Methods
Glyceride Analysis Natural Products and Supplements
Triglyceride Analysis
Title Authors Publication Publication Date
Poly-Unsaturated Fatty Acids Simultaneous determination of triterpenoid saponins from pulsatilla koreana
Bull. Korean Chem. Soc.
using high performance liquid chromatography coupled with a charged aerosol Yeom, H.; Suh, J. H.; Youm, J. R.; Han, S. B. 2010
31 (5), 1159–1164
Antioxidant Additives detector (HPLC-CAD)
DPPH radical scavenging activities of 31 flavonoids and phenolic acids and Zhongguo Zhongyao Zazhi
Yuan, Y.; Chen, C.; Yang, B.; Kusu, F.; Kotani, A. 2009 Jul
References 10 extracts of Chinese materia medica 34 (13), 1695–1700
Determination of residual clenbuterol in pork meat and liver by HPLC with Yaoxue Xuebao 39 (4),
Zhang, X. Z.; Gan, Y. R.; Zhao, F. N. 2004 Apr
electrochemical detection 276–280
Identification of equol producers in a Japanese population by high-performance Zhao, J. H.; Sun, S. J.; Arao, Y.; Oguma, E.; Phytomedicine 13 (5),
2006 May
liquid chromatography with coulometric array for determining serum isoflavones Yamada, K.; Horiguchi, H.; Kayama, F. 304–309
Zhu, L.; Hu, Z.; Gamez, G.; Law, W. S.; Chen, H.;
Simultaneous sampling of volatile and non-volatile analytes in beer for fast Anal. Bioanal. Chem. 398 (1),
Yang, S.; Chingin, K.; Balabin, R. M.; Wang, R.; 2010 Sep
fingerprinting by extractive electrospray ionization mass spectrometry 405–413
Zhang, T.; Zenobi, R.
Zitka, O.; Sochor, J.; Rop, O.; Skalickova, S.;
Comparison of various easy-to-use procedures for extraction of phenols from
Sobrova, P.; Zehnalek, J.; Beklova, M.; Krska, B.; Molecules 16 (4), 2914–2936 2011 Apr 4
apricot fruits
Adam, V.; Kizek, R.
 References

Table of Contents

Introduction Peer Reviewed Journals:

Analytical Technologies HPLC and UHPLC Methods
Glyceride Analysis Lipids
Triglyceride Analysis
Title Authors Publication Publication Date
Poly-Unsaturated Fatty Acids Development of analytical procedures to study changes in the composition of J. Chromatogr., A 1120 (1–2),
Cascone, A.; Eerola, S.; Ritieni, A.; Rizzo, A. 2006 Jul 7
meat phospholipids caused by induced oxidation 211–220
Antioxidant Additives Chapter 5. In Hyphenated
and Alternative Methods of
References Detection in Chromatography,
Evaporative light scattering and charged aerosol detector. Chaminade, P. Chromatographic Science 2012
Series; Shalliker, A., Ed.; Taylor
& Francis Group, LLC: Boca
Raton, FL.; 145–160
Simple and efficient profiling of phospholipids in phospholipase D-modified soy J. Am. Oil Chem. Soc. 90 (7),
Damnjanovic´, J.; Nakano, H.; Iwasaki, Y. 2013 Jul
lecithin by HPLC with charged aerosol detection 951–957
de la Mata-Espinosa, P.; Bosque-Sendra, J. M.; Anal. Bioanal. Chem. 399 (6),
Discriminating olive and non-olive oils using HPLC-CAD and chemometrics 2011 Feb
Bro, R.; Cuadros-Rodríguez, L. 2083–2092
Olive oil quantification of edible vegetable oil blends using triacylglycerols de la Mata-Espinosa, P.; Bosque-Sendra, J. M.;
Talanta 85 (1), 177–182 2011 Jul 15
chromatographic fingerprints and chemometric tools Bro, R.; Cuadros-Rodríguez, L.
de la Mata-Espinosa, P.; Bosque-Sendra, J.; Food Analytical Methods 4 (4),
Quantification of triacylglycerols in olive oils using HPLC-CAD 2011 Dec
Cuadros-Rodriguez, L. 574–581
Quantification of pegylated phospholipids decorating polymeric microcapsules
Díaz-López, R.; Libong, D.; Tsapis, N.; Fattal, E.; J. Pharm. Biomed. Anal.
of perfluorooctyl bromide by reverse phase HPLC with a charged aerosol 2008 Nov 4
Chaminade, P. 48 (3), 702–707
detector
Molecules 14 (9),
Squalene emulsions for parenteral vaccine and drug delivery Fox, C. B. 2009 Sep 1
3286–3312
Gonyon, T.; Tomaso, A.; Kotha, P.; Owen, H.; PDA J. Pharm. Sci. and Tech.
Interactions between parenteral lipid emulsions and container surfaces 2013 May–Jun
Patel, D.; Carter, P.; Cronin, J.; Green, J. 67 (3), 247–254
Composition analysis of positional isomers of phosphatidylinositol by high- Iwasaki, Y.; Masayama, A.; Mori, A.; Ikeda, C.; J. Chromatogr., A 1216 (32),
2009 Aug 7
performance liquid chromatography Nakano, H. 6077–6080
Chinese Pharmaceutical
Determination of phospholipid and its degradation products in liposomes for Journal (Zhongguo Yaoxue
Jiang, Q.; Yang, R.; Mei, X. 2007
injection by HPLC-charged aerosol detection (CAD) Zazhi, Beijing, China) 42 (23),
1794–1796
 References

Table of Contents

Introduction Peer Reviewed Journals:

Analytical Technologies HPLC and UHPLC Methods
Glyceride Analysis Lipids
Triglyceride Analysis
Title Authors Publication Publication Date
Poly-Unsaturated Fatty Acids Rapid quantification of yeast lipid using microwave-assisted total lipid extraction Khoomrung, S.; Chumnanpuen, P.; Jansa-Ard, S.; Anal. Chem. 85 (10),
2013 May 21
and HPLC-CAD Ståhlman, M.; Nookaew, I.; Borén, J.; Nielsen, J. 4912–4919
Antioxidant Additives A new liquid chromatography method with charge aerosol detector (CAD) for
Kieibowicz, G.; Micek, P.; Wawrzenczyk, C. Talanta 105, 28–33 2013 Feb 15
the determination of phospholipid classes. Application to milk phospholipids
References An LC method for the analysis of phosphatidylcholine hydrolysis products and Kiełbowicz, G.; Smuga, D.; Gładkowski, W.;
Talanta 94, 22–29 2012 May 30
its application to the monitoring of the acyl migration process Chojnacka, A.; Wawrzen´czyk, C.
Separation of acylglycerols, FAME and FFA in biodiesel by size exclusion Eur. J. Lipid Sci. Technol.
Kittirattanapiboon, K.; Krisnangkurá, K. 2008 Mar 17
chromatography 110 (5), 422–427
Quantitation of triacylglycerols from plant oils using charged aerosol detection J. Chromatogr., A. 1176 (1–2),
Lísa, M.; Lynen, F.; Holcˇapek, M.; Sandra, P. 2007 Dec 28
with gradient compensation 135–142
Quantitative study of the stratum corneum lipid classes by normal phase liquid Merle, C.; Laugel, C.; Chaminade, P.; J. Liq. Chromatogr. Relat.
2010 Mar
chromatography: comparison between two universal detectors Baillet-Guffroy, A. Technol. 33, 629–644
The analysis of lipids via HPLC with a charged aerosol detector Moreau, R. A. Lipids 41 (7), 727–34 2006 Jul
Lipid Technol. 21 (8–9),
Lipid analysis via HPLC with a charged aerosol detector Moreau, R. A. 2009 Oct 23
191–194
Chapter 6 in Methods of
Analysis of Food Components
and Additives, Second Edition;
Extraction and analysis of food lipids Moreau, R. A.; Winkler-Moser, J. K. 2011 Nov
Ötles, S., Ed.; Taylor & Francis
Group, LLC: Boca Raton, FL.;
115–134
Aerosol based detectors for the investigation of phospholipid hydrolysis in a J. Pharm. Biomed. Anal.
Nair, L.; Werling, J. 2009 Jan 15
pharmaceutical suspension formulation 49 (1), 95–99
Pang, X. Y.; Cao, J.; Addington, L.; Lovell, S.;
Structure/function relationships of adipose phospholipase A2 containing a cys- J. Biol. Chem. 287 (42),
Battaile, K. P.; Zhang, Rao, J. L.; Dennis, E. A.; 2012 Oct 12
his-his catalytic triad 35260–35274
Moise, A. R.
Simultaneous assessment of lipid classes and bile acids in human intestinal fluid Persson, E.; Löfgren, L.; Hansson, G.;
J. Lipid Res. 48 (1), 242–251 2007 Jan
by solid-phase extraction and HPLC methods Abrahamsson, B.; Lennernäs, H.; Nilsson, R.
 References

Table of Contents

Introduction Peer Reviewed Journals:

Analytical Technologies HPLC and UHPLC Methods
Glyceride Analysis Lipids
Triglyceride Analysis
Title Authors Publication Publication Date
Poly-Unsaturated Fatty Acids Methods Mol. Biol. (Totowa,
The use of charged aerosol detection with HPLC for the measurement of lipids Plante, M.; Bailey, B.; Acworth, I. 2009
NJ, U.S.) 579, 469–482
Antioxidant Additives Comparison between charged aerosol detection and light scattering detection Ramos, R. G.; Libong, D.; Rakotomanga, M.; J. Chromatogr., A. 1209 (1–2),
2008 Oct 31
for the analysis of Leishmania membrane phospholipids Gaudin, K.; Loiseau, P. M.; Chaminade, P. 88–94
References Authentication of geographical origin of palm oil by chromatographic Ruiz-Samblás, C.; Arrebola-Pascual, C.; Tres, A.;
Talanta. 116, 788–793 2013 Nov 15
fingerprinting of triacylglycerols and partial least square-discriminant analysis van Ruth, S.; Cuadros-Rodríguez, L.
Simple and precise detection of lipid compounds present within liposomal Schönherr, C.; Touchene, S.; Wilser, G.; J. Chromatogr., A. 1216 (5),
2009 Jan 30
formulations using a charged aerosol detector Peschka-Süss, R.; Francese, G. 781–786
Determination of intralumenal individual bile acids by HPLC with J. Lipid Res. 49 (12),
Vertzoni, M.; Archontaki, H.; Reppas, C. 2008 Dec
charged aerosol detection 2690–2695
Am. Biotechnol. Lab. 26 (1),
Neurolipids and the use of a charged aerosol detector Waraska, J.; Acworth, I. 2008
12–13
 References

Table of Contents

Introduction Technical Collateral:

Analytical Technologies HPLC and UHPLC Methods
Glyceride Analysis Product Number Technique Title
AB 119 UV Rapid Separation of Paclitaxel and Related Compounds in Paclitaxel Injection
Triglyceride Analysis
AB 134 MS LC-MS Analysis of Anthocyanins in Bilberry Extract
Poly-Unsaturated Fatty Acids AB 139 UV Separation of Schizandrin, Schizandrin A, and Schizandrin B in a Tablet Sample
AB 153 UV Save the Flavor – Robust Iso-α-Acids Assaying in Beer within Ten Minutes
Antioxidant Additives AB 155 UV Monitor the Brewing Process with LC-Transformation of Hop alpha-Acids into Beer Iso-alpha-Acids
AN 109 FLD Determination of Glyphosate by Cation-Exchange Chromatography with Postcolumn Derivatization
References
AN 156 UV The Everlasting Paradigm-Keep Beer Tradition or Prevent Beer from a Skunky Off-Flavor?
AN 196 FLD Determination of Polycyclic Aromatic Hydrocarbons (PAHs) in Edible Oils by Donor-Acceptor Complex Chromatography (DACC)-HPLC with Fluorescent Detection
AN 207 UV Chromatographic Fingerprinting of Flos Chrysanthema indici Using HPLC
AN 213 UV/FLD Determination of Polycyclic Aromatic Hydrocarbons (PAHs) in Tap Water Using on-Line Solid-Phase Extraction Followed by HPLC with UV and Fluorescence Detections
AN 216 UV Determination of Water- and Fat-Soluble Vitamins in Functional Waters by HPLC with UV-PDA Detection
AN 224 UV Determination of Melamine in Milk Powder by Reversed-Phase HPLC with UV Detection
AN 232 UV Determination of Anthraquinones and Stilbenes in Giant Knotweed Rhizome by HPLC with UV Detection
AN 236 UV Determination of Iodide and Iodate in Seawater and Iodized Table Salt by HPLC-UV Detection
AN 245 UV Fast Analysis of Dyes in Foods and Beverages
AN 251 UV Determination of Water- and Fat-Soluble Vitamins in Nutritional Supplements by HPLC with UV Detection
AN 252 UV HPLC Assay of Water-Soluble Vitamins, Fat-Soluble Vitamins, and a Preservative in Dry Syrup Multivitamin Formulation
AN 261 UV Sensitive Determination of Microcystins in Drinking and Environmental Waters
AN 264 UV Fast Determination of Anthocyanins in Pomegranate Juice
AN 266 FLD Determination of Sialic Acids Using UHPLC with Fluorescence Detection
AN 272 FLD Faster Yet Sensitive Determination of N-Methylcarbamates in Rice, Potato, and Corn by HPLC
AN 275 UV Sensitive Determination of Catechins in Tea by HPLC
AN 287 UV Two-Dimensional HPLC Combined with On-Line SPE for Determination of Sudan Dyes I–IV in Chili Oil
AN 292 UV Determination of Aniline and Nitroanilines in Environmental and Drinking Waters by On-Line SPE
AN 293 CAD and UV Steviol Glycoside Determination by HPLC with Charged Aerosol and UV Detections Using the Acclaim Trinity P1 Column
AN 299 UV HPLC Analysis of Six Active Components of Caulis lonicerae Using a Phenyl-1 Column
AN 1008 UV Determination of Nitidine Chloride, Toddalolactone, and Chelerythrine Chloride by HPLC
 References

Table of Contents

Introduction Technical Collateral:

Analytical Technologies HPLC and UHPLC Methods
Glyceride Analysis Product Number Technique Title
AN 1020 EC, UV Chalcinoids and Bitter Acids in Beer by HPLC with UV and ECD
Triglyceride Analysis
AN 1023 UV Determination of Sudan Dyes I–IV in Curry Paste
Poly-Unsaturated Fatty Acids AN 1026 CAD Fatty Acid Esters at Low Nanogram Levels
AN 1027 CAD Ginseng
Antioxidant Additives AN 1028 CAD Ginkgo biloba
AN 1029 CAD Black Cohosh
References
AN 1030 CAD Soy Saponins
AN 1032 CAD Unsaturated Fatty Acid: Arachidonic, Linoleic, Linolenic and Oleic Acids
AN 1033 CAD Corn Syrup
AN 1034 CAD Honey Sugars
AN 1035 CAD Phenolic Acids
AN 1036 CAD Water-Soluble Antioxidants: Ascorbic Acid, Glutathione and Uric Acid
AN 1037 CAD Artificial Sweeteners-Global Method
AN 1039 CAD Simultaneous Measurement of Glycerides (Mono-, Di- and Triglycerides) and Free Fatty Acids in Palm Oil
AN 1040 CAD Analysis of Commercially Available Products Containing Stevia
AN 1041 CAD Phytosterols
AN 1042 UV Rapid Separation of Anthocyanins in Cranberry and Bilberry Extracts Using a Core-Shell Particle Column
AN 1045 UV Determination of Phthalates in Drinking Water by UHPLC with UV Detection
AN 1046 UV Determination of Phenylurea Compounds in Tap Water and Bottled Green Tea
AN 1055 CAD Determination of Virginiamycin, Erythromycin, and Penicillin in Dried Distillers Grains with Solubles
AN 1063 ECD Targeted Analyses of Secondary Metabolites in Herbs, Spices, and Beverages Using a Novel Spectro-Electro Array Platform
AN 1064 ECD Product Authentication and Adulteration Determination Using a Novel Spectro-Electro Array Platform
AN 1067 UV Determination of Carbendazim in Orange Juice
AN 1069 UV Two-Dimensional HPLC Determination of Water-Soluble Vitamins in a Nutritional Drink
AN 1070 UV Determination of Inositol Phosphates in Dried Distillers Grains and Solubles
AN 20583 UV Determination of Catechins and Phenolic Acids in Red Wine by Solid Phase Extraction and HPLC
AN 20610 UV Fast Analysis of Coffee Bean Extracts Using a Solid Core HPLC Column
AN 20663 CAD Comparative Analysis of Cooking Oils Using a Solid Core HPLC Column
AN 20847 CAD Analysis of a Sports Beverage for Electrolytes and Sugars Using Multi-Mode Chromatography with Charged Aerosol Detection
 References

Table of Contents

Introduction Technical Collateral:

Analytical Technologies HPLC and UHPLC Methods
Glyceride Analysis Product Number Technique Title
AN 70158 CAD Novel Universal Approach for the Measurement of Natural Products in a Variety of Botanicals and Supplements
Triglyceride Analysis
AN 70277 CAD Simultaneous Analysis of Glycerides and Fatty Acids in Palm Oil
Poly-Unsaturated Fatty Acids AU 144 UV Determination of Hexavalent Chromium in Drinking Water Using Ion Chromatography
AU 170 UV Fast Determination of Vanillin and its Synthesis Precursor by HPLC
Antioxidant Additives AU 182 CAD Measuring Lactose in Milk: A Validated Method
AU 184 CAD, UV Mogroside V Determination by HPLC with Charged Aerosol and UV Detections
References
CAN 106 UV Determination of the Punicalagins Found in Pomegranate by High Performance Liquid Chromatography
CAN 111 CAD Determination of Triterpenes in Centella asiatica (Gotu Kola) by HPLC-CAD
CAN 112 CAD Determination of Ginsenosides in Panax ginseng by HPLC-CAD
CAN 115 FLD Clean-Up and Analysis of Aflatoxins and Ochratoxin A in Herbs and Spices
LPN 2062 MS Profiling Analysis of 15 Prominent Naturally Occurring Phenolic Acids by LC-MS
LPN 2069 FLD Fast and Effective Determination of Aflatoxins in Grains or Food Using Accelerated Solvent Extraction followed by HPLC
LPN 2421 UV Achieving Maximum Productivity by Combining UHPLC with Advanced Chromatographic Techniques
LPN 2818 CAD Analysis of Fat-Soluble Vitamins and Antioxidants in Supplements by RP-HPLC
LPN 2870 FLD Benefits of High-Speed Wavelength Switching in UHPLC Methods Using Fluorescence Detection
LPN 2930 CAD Determination of the Composition of Natural Products by HPLC with Charged Aerosol Detection
LPN 2923 CAD Simple and Direct Analysis of Falcarinol and Other Polyacetylenic Oxylipins in Carrots by Reversed-Phase HPLC and Charged Aerosol Detection
LPN 2931 CAD Quantification of Underivatized Omega-3 and Omega-6 Fatty Acids in Foods by HPLC CAD
LPN 2932 ECD A Versatile Detector for the Sensitive and Selective Measurement of Numerous Fat-Soluble Vitamins and Antioxidants in Human Plasma and Plant Extracts
LPN 2934 CAD Sensitive Analysis of Commonly Used Artificial and Natural Sweeteners Including Stevia and Their Impurities and Degradation Products
Evaluation of Methods for the Characterization and Quantification of Polysorbates and Impurities Along with Other Surfactants and Emulsifiers Used in the Food
LPN 2991 CAD
and Pharmaceutical Industries
PN 70026 CAD Carbohydrate Analysis Using PAD, FLD, CAD and MS Detectors
PN 70037 CAD Sensitive HPLC Method for Triterpenoid Analysis Using Charged Aerosol Detection with Improved Resolution
PN 70055 CAD Direct Analysis of Surfactants using HPLC with Charged Aerosol Detection
PN 70138 UV Rapid Determination of Polyphenol Antioxidants in Green Tea and Cranberry Extract Using Core Shell Columns
PN 70538 CAD Analysis of Silicone Oils by HPLC-CAD
PN 70540 CAD, ECD Profiling Hoodia Extracts by HPLC with CAD, ECD, Principal Component Analysis
Table of Contents

Introduction

Analytical Technologies

Glyceride Analysis

Triglyceride Analysis References

Poly-Unsaturated Fatty Acids

Antioxidant Additives

References

Ion Chromatography References

 References

Table of Contents

Introduction Technical Collateral:

Analytical Technologies Ion Chromatography Methods
Glyceride Analysis Product Number Technique Title
AB 127 IC-PAD Determination of Carbohydrates in Fruit Juice Using Capillary High-Performance Anion-Exchange Chromatography
Triglyceride Analysis
AB 135 IC-SC Determination of Anions and Organic Acids in Brewed Coffee Samples Using Capillary IC
Poly-Unsaturated Fatty Acids AB 137 IC-SC Determination of Inorganic and Organic Acids in Apple and Orange Juice Samples Using Capillary IC
AN 25 IC-SC Determination of Inorganic Ions and Organic Acids in Non-Alcoholic Carbonated Beverages
Antioxidant Additives AN 37 IC-PAD Determination of Iodide and Iodate in Soy- and Mil-Based Infant Formulas
AN 46 IC-PAD Ion Chromatography: A Versatile Technique for the Analysis of Beer
References
AN 54 IC-PAD Determination of Total and Free Sulfite in Foods and Beverages
AN 67 IC-PAD Determination of Plant-Derived Neutral Oligo- and Polysaccharides
AN 81 IC-SC Ion Chromatographic Determination of Oxyhalides and Bromide at Trace Level Concentrations in Drinking Water Using direct Injection
AN 82 IC-PAD Analysis of Fruit Juice Adulterated with Medium Invert Sugar from Beets
AN 87 IC-PAD Determination of Sugar Alcohols in Confections and Fruit Juices by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection
AN 101 IC-SC Trace Level Determination of Bromate in Ozonated Drinking Water Using Ion Chromatography
AN 112 IC-UV Determination of Nitrate and Nitrite in Meat Using High-Performance Anion-Exchange Chromatography
AN 121 IC-SC Analysis of Low Concentrations of Perchlorate in Drinking Water and Ground Water by Ion Chromatography
AN 123 IC-SC Determination of Inorganic Anions and Organic Acids in Fermentation Broths
AN 133 IC-SC Determination of Inorganic Anions in Drinking Water by Ion Chromatography
IC-SC and Determination of Inorganic Oxyhalide Disinfection Byproduct Anions and Bromide in Drinking Water Using Ion Chromatography with the Addition of a Postcol-
AN 136
IC-UV umn Reagent for Trace Bromate Analysis
AN 140 IC-SC Fast Analysis of Anions in Drinking Water by Ion Chromatography
AN 143 IC-SC Determination of Organic Acids in Fruit Juices
Determination of Chlorite, Bromate, Bromide, and Chlorate in Drinking Water by Ion Chromatography with an On-Line-Generated Postcolumn Reagent for
AN 149 IC-SC
Sub-µg/L Bromate Analysis
AN 150 IC-PAD Determination of Amino Acids in Cell Cultures and Fermentation Broths
AN 154 IC-SC Determination of Inorganic Anions in Environmental Waters Using a Hydroxide-Selective Column
AN 155 IC-PAD Determination of Trans-Galactooligosaccharides in Foods by AOAC Method 2001.02
 References

Table of Contents

Introduction Technical Collateral:

Analytical Technologies Ion Chromatography Methods
Glyceride Analysis Product Number Technique Title
AN 165 IC-SC Determination of Benzoate in Liquid Food Products by Reagent-Free Ion Chromatography
Triglyceride Analysis
Determination of Trace Concentrations of Oxyhalides and Bromide in Municipal and Bottled Waters Using a Hydroxide-Selective Column with a Reagent-Free
AN 167 IC-SC
Ion Chromatography System
Poly-Unsaturated Fatty Acids
Determination of Trace Concentrations of Disinfection By-Product Anions and Bromide in Drinking Water Using Reagent-Free Ion Chromatography Followed by
AN 168 IC-UV
Postcolumn Addition of IoI-Dianisidine for Trace Bromate Analysis
Antioxidant Additives
AN 169 IC-SC Rapid Determination of Phosphate and Citrate in Carbonated Soft Drinks Using a Reagent-Free Ion Chromatography System
References AN 172 IC-SC Determination of Azide in Aqueous Samples by Ion Chromatography with Suppressed Conductivity Detection
AN 173 IC-PAD Direct Determination of Cyanide in Drinking Water by Ion Chromatography with Pulsed Amperometric Detection (PAD)
Improved Determination of Trace Concentrations of Perchlorate in Drinking Water Using Preconcentration with Two-Dimensional Ion Chromatography and
AN 178 IC-SC
Suppressed Conductivity Detection
IC-SC and Determination of Biogenic Amines in Alcoholic Beverages by Ion Chromatography with Suppressed Conductivity and Integrated
AN 182
IC-PAD Pulsed Amperometric Detections
IC-SC and Determination of Biogenic Amines in Fermented and Non-Fermented Foods Using Ion Chromatography with Suppressed Conductivity and Integrated Pulsed
AN 183
IC-PAD Amperometric Detections
Determination of sub-µg/L Bromate in Municipal Waters Using Preconcentration with Two-Dimensional Ion Chromatography and
AN 187 IC-SC
Suppressed Conductivity Detection
AN1 88 IC-PAD Determination of Glycols and Alcohols in Fermentation Broths Using Ion-Exclusion Chromatography and Pulsed Amperometric Detection
AN 197 IC-PAD Determination of Glucosamine in Dietary Supplements Using HPAE-PAD
Determination of Total Cyanide in Municipal Wastewater and Drinking Water Using Ion-Exclusion Chromatography with Pulsed Amperometric Detection
AN 227 ICE-PAD
(ICE-PAD)
AN 248 IC-PAD Determination of Lactose in Lactose-Free Milk Products by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection
AN 253 IC-PAD HPAE-PAD Determination of Infant Formula Sialic Acids
AN 270 IC-PAD Determination of Hydroxymethylfurfural in Honey and Biomass
AN 273 IC-SC Determination of Organic Acids in Fruit Juices and Wines by High-Pressure IC
AN 279 IC-SC Time Savings and Improved Reproducibility of Nitrate and Nitrite Ion Chromatography Determination in Milk Samples
AN 280 IC-PAD Carbohydrates in Coffee: AOAC Method 995.13 vs a New Fast Ion Chromatography Method
AN 295 IC-SC Determination of Phytic Acid in Soybeans and Black Sesame Seeds
AN 1007 IC-SC Determination of Mono-, Di-, and Triphosphates and Citrate in Shrimp by Ion Chromatography
 References

Table of Contents

Introduction Technical Collateral:

Analytical Technologies Ion Chromatography Methods
Glyceride Analysis Product Number Technique Title
AN 1044 IC-SC Determination of Anions in Dried Distillers Grains with Solubles
Triglyceride Analysis
AN 1068 IC-SC Determination of Organic Acids in Fruit Juices and Wines by High-Pressure IC
Poly-Unsaturated Fatty Acids AU 132 IC-UV Determination of Nitrite and Nitrate in drinking Water by Ion Chromatography with Direct UV Detection
AU 144 IC-UV Determination of Hexavalent Chromium in Drinking Water Using Ion Chromatography
Antioxidant Additives AU 148 IC-SC Determination of Perchlorate in Drinking Water Using Reagent-Free Ion Chromatography
AU 150 IC-PAD Determination of Plant-Derived Neutral Oligo- and Polysaccharides Using the CarboPac PA200
References
AU 151 IC-PAD Determination of Sucralose in Reduced- Carbohydrate Colas using High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection
AU 189 IC-SC Determination of Choline in Infant Formula and Other Food Samples by IC
LPN 2982 IC-SC Determination of Inorganic Anions and Organic Acids in Beverages Using a Capillary IC on a Monolith Anion-Exchange Column
PN 70743 IC-SC Determination of Perchlorate Levels in Food and Soil Samples Using Accelerated Solvent Extraction and Ion Chromatography
TN 20 IC-PAD Analysis of Carbohydrates by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD)
TN 126 IC-SC Determination of Organic Acids in Beer Samples Using a High-Pressure Ion Chromatography System
TN 135 IC-PAD Determinations of Monosaccharides and Disaccharides in Beverages by Capillary HPAE-PAD
Table of Contents

Introduction

Analytical Technologies

Glyceride Analysis

Triglyceride Analysis References

Poly-Unsaturated Fatty Acids

Antioxidant Additives

References

Sample Preparation References

 References

Table of Contents

Introduction Peer Reviewed Journals:

Analytical Technologies Sample Preparation Methods
Glyceride Analysis Title Authors Publication Publication Date
Accelerated, microwave-assisted, and conventional solvent extraction methods
Triglyceride Analysis Abdel-Aal el-SM; Akhtar, H.; Rabalski, I.; Bryan, M. J. Food Sci. 79 (2), C138–46 2014 Feb
affect anthocyanin composition from colored grains
Multiresidue method for the analysis of pesticide residues in fruits and vegetables J. Agric. Food Chem. 49 (9),
Poly-Unsaturated Fatty Acids Adou, K.; Bontoyan, W. R.; Sweeney, P. J. 2001 Sep
by accelerated solvent extraction and capillary gas chromatography 4153–4160
J. Chromatogr. B Analyt. Technol.
Antioxidant Additives The development of an optimized sample preparation for trace level detection of Al-Ansari, A. M.; Saleem, A.; Kimpe, L. E.; Trudeau,
Biomed. Life Sci. 879 (30), 2011 Nov
17α-ethinylestradiol and estrone in whole fish tissue V. L.; Blais, J. M.
3649–52
References
Determination of polyphenolic profiles of basque cider apple varieties using accel- Alonso-Salces, R. M.; Korta, E.; Barranco, A.; J. Agric. Food Chem. 49 (8),
2001
erated solvent extraction Berrueta, L.A.; Gallo, B.; Vicent, F. 3761–376
Alonso-Salces, R. M.; Korta, E.; Barranco, A.; J. Chromatogr., A. 933 (1–2),
Pressurized liquid extraction for the determination of polyphenols in apple 2001 Nov
Berrueta, L. A.; Gallo, B.; Vicente, F.; 37–43
Methods for extraction and determination of phenolic acids in medicinal plants: a Nat. Prod. Commun. 8 (12),
Arceusz, A.; Wesolowski, M.; Konieczynski, P. 2013 Dec
review 1821–9
Study of an accelerated solvent extraction procedure for the determination of
J. Food Protection 65 (1),
acaricide residues in honey by high-performance liquid chromatography-diode Bakkali, A.; Korta, E.; Berrueta, L. A. 2002
161–166
array detector
J. Chromatogr., A. 837 (1-2),
Pressurized liquid extraction of medicinal plants Benthin, B.; Danz, H.; Hamburger, M. 1999 Apr
211–9
Comparison of the chemical composition of extracts from Scutellaria lateriflora us-
J. Agric. Food Chem. 53 (8),
ing accelerated solvent extraction and supercritical fluid extraction versus standard Bergeron, C.; Gafner, S.; Clausen, E.; Carrier, D. J. 2005 Apr
3076–80
hot water or 70% ethanol extraction
Polybrominated diphenyl ethers (PBDEs) in Mediterranean mussels (Mytilus gallo- Bianco, G.; Novario, G.; Anzilotta, G.; Palma, A.; J. Mass Spectrom. 45 (9),
2010 Sep
provincialis) from selected Apulia coastal sites evaluated by GC-HRMS Mangone, A.; Cataldi, T. R. 1046–55
Free and bound phenolic compounds in barley (Hordeum vulgare L.) flours. evalu-
J. Chromatogr., A.19; 1057
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Analytical Technologies Sample Preparation Methods
Glyceride Analysis Title Authors Publication Publication Date
Cervera, M.I.; Medina, C.; Portolés, T.; Pitarch, E;
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Poly-Unsaturated Fatty Acids Influence of extraction methodologies on the analysis of five major volatile
Chanthai, S.; Prachakoll, S.; Ruangviriyachai, C.;
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Antioxidant Additives Luthria, D. L.
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Development of a liquid chromatography-tandem mass spectrometry with pres- Chen, D.; Tao, Y.; Zhang, H.; Pan, Y.; Liu, Z.; J. Chromatogr. B Analyt. Technol.
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Determination of 88 pesticide residues in tea using gas chromatography-tandem
Chen, H.; Liu, X.; Wang, Q.; Jiang, Y. Se Pu. 29 (5), 409–16 2011 May
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Optimization of accelerated solvent extraction for the determination of chlorinated Chen, S.; Gfrerer, M.; Lankmayr, E.; Quan, X.;
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Chitescu, C. L.; Nicolau, A. I.; Stolker, A. A. Anal. Control Expo. Risk Assess. 2013
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Cicchetti, E.; Chaintreau, A.. J. Sep. Sci. 32 (11), 1957–64 2009 Jun
traction for the authentication of natural vanilla flavors
Development of a fast and convenient method for the isolation of triterpene sapo-
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Introduction Peer Reviewed Journals:

Analytical Technologies Sample Preparation Methods
Glyceride Analysis Title Authors Publication Publication Date
Pressurized fluid extraction of carotenoids from Haematococcus pluvialis and Anal. Chim. Acta. 501 (2),
Triglyceride Analysis Denery, J. R.; Dragull, K.:Tang, C. S.; Li, Q. X. 2004 Jan
Dunaliella salina and kavalactones from Piper methysticum 175–181
Development and comparison of two multiresidue methods for the analysis of
Poly-Unsaturated Fatty Acids Desmarchelier, A.; Oberson, J. M.; Tella, P.; J. Agric. Food Chem. 58 (13),
17 mycotoxins in cereals by liquid chromatography electrospray ionization tandem 2010 Jul
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Identification, extraction and quantification of the synthetic cannabinoid JWH-018 Forensic Sci. Int. 223 (1-3),
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 References

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Analytical Technologies Sample Preparation Methods
Glyceride Analysis Title Authors Publication Publication Date
Multianalysis of 35 mycotoxins in traditional Chinese medicines by ultra-high-per-
Triglyceride Analysis Han, Z.; Ren Y.; Zhu, J.; Cai, Z.; Chen, Y.; Luan, L.; J. Agric. Food Chem. 60 (33),
formance liquid chromatography-tandem mass spectrometry coupled with 2012 Aug
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Pressurized liquid extraction-capillary electrophoresis-mass spectrometry for the J. Chromatogr., A.1084 (1-2),
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Accelerated solvent extraction of alkylresorcinols in food products containing Holt, M D.; Moreau, R A,.;DerMarderosian, A.; J. Agric. Food Chem. 60 (19),
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extraction of antioxidant compounds from sage (Salvia officinalis L.), basil (Ocimum Food Funct. 1(3), 269–77 2010 Dec
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A review of modern sample-preparation techniques for the extraction and analysis Anal. Bioanal. Chem. 373 (1-2),
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 References

Table of Contents

Introduction Peer Reviewed Journals:

Analytical Technologies Sample Preparation Methods
Glyceride Analysis Title Authors Publication Publication Date
Juan, C.; González, L.; Soriano, J. M.; Moltó, J. C.; J. Agric. Food Chem. 53 (24),
Triglyceride Analysis Accelerated solvent extraction of ochratoxin A from rice samples 2005
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Antioxidant Additives Determination of polybromodiphenyl ethers (PBDEs) in milk cream by gas chroma- Kinani, S.; Bouchonnet, S.; Abjean, J.;
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Table of Contents

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Analytical Technologies Sample Preparation Methods
Glyceride Analysis Title Authors Publication Publication Date
J. Agric. Food Chem. 54 (1),
Triglyceride Analysis Influence of sample preparation on assay of phenolic acids from eggplant Luthria, DL.; Mukhopadhyay, S. 2006
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Poly-Unsaturated Fatty Acids Pressurised solvent extraction for organotin speciation in vegetable matrices Marcic, C.; Lespe,s G.; Potin-Gautier, M. 2005 Aug
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Analytical Technologies Sample Preparation Methods
Glyceride Analysis Title Authors Publication Publication Date
Determination of organophosphorus pesticides in foods using an accelerated
Triglyceride Analysis Obana, H.; Kikuchi, K.; Okihashi, M.; Hori, S. Analyst 122 (3), 217–20 1997 Mar
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 References

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Introduction Peer Reviewed Journals:

Analytical Technologies Sample Preparation Methods
Glyceride Analysis Title Authors Publication Publication Date
An improved clean-up strategy for simultaneous analysis of polychlorinated diben-
Triglyceride Analysis Anal. Bioanal. Chem. 372 ( 2),
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Tian, S.; Mao, X.; Miao, S.; Jia, Z.; Wang, K.; Ji, S. Se Pu. 30 (1), 14–20. 2012 Jan
chromatography tandem mass spectrometry
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Table of Contents

Introduction Peer Reviewed Journals:

Analytical Technologies Sample Preparation Methods
Glyceride Analysis Title Authors Publication Publication Date
A consecutive preparation method based upon accelerated solvent extraction and
Triglyceride Analysis high-speed counter-current chromatography for isolation of aesculin from Cortex Tong, X.; Zhou, T, Xiao, X.; Li, G. J. Sep. Sci. 35 (24), 3609–14 2012 Dec
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Antioxidant Additives toes by HPLC-DAD/ESI-MS/MS 404–10
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Fat extraction from acid- and base-hydrolyzed food samples using accelerated Ullah, S. M.; Murphy, B.; Dorich, B.; Richter, B.; J. Agric. Food Chem. 59 (6),
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Subcritical water extraction of alkaloids in Sophora flavescens Ait. and determina- J. Pharm. Biomed .Anal. 58,
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Evaluation of Soxhlet extraction, accelerated solvent extraction and microwave-as-
Wang, P.; Zhang, Q.; Wang, Y.; Wang, T.; Li X.; Ding,
sisted extraction for the determination of polychlorinated biphenyls and polybromi- Anal. Chim. Acta. 663 (1), 43–8. 2010 Mar
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nated diphenyl ethers in soil and fish samples
Determination of ten pesticides of pyrazoles and pyrroles in tea by accelerated Xu, D.; Lu, S.; Chen, D.; Lan, J.; Zhang, Z.; Yang,
Se Pu.; 31 (3), 218–22. 2013 Mar
solvent extraction coupled with gas chromatography-tandem mass spectrometry F.; Zhou, Y.
Online cleanup of accelerated solvent extractions for determination of adenosine
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phosphate (AMP) in royal jelly using high-performance liquid chromatography
Identification and quantitation of eleven sesquiterpenes in three species of
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Curcuma rhizomes by pressurized liquid extraction and gas chromatography– 2005 Sep
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spectrometry with accelerated solvent extraction 150–9.
 References

Table of Contents

Introduction Peer Reviewed Journals:

Analytical Technologies Sample Preparation Methods
Glyceride Analysis Title Authors Publication Publication Date
Determination of pentachlorophenol residue in meat and fish by gas chromatogra-
Triglyceride Analysis phy-electron capture detection and gas chromatography-mass spectrometry with Zhao, D. J. Chromatogr. Sci. 2013 May
accelerated solvent extraction
Poly-Unsaturated Fatty Acids Response surface modeling and optimization of accelerated solvent extraction of Zhao, L. C.; He, Y, Deng.; X, Yang, G. L.; Li, W.;
Molecules. 17 (4), 3618–29 2012 Mar
four lignans from fructus schisandrae Liang, J.; Tang, Q. L.
Antioxidant Additives
Determination of acetanilide herbicides in cereal crops using accelerated solvent
extraction, solid-phase extraction and gas chromatography-electron capture Zhang, Y.; Yang, J.; Shi, R.; Su, Q.; Yao, L.; Li, P. J. Sep. Sci. 34 (14), 1675–82 2011 Jul
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constituents from Hypericum perforatum L
Analysis of volatile components in Qingshanlvshui tea using solid-phase microex- Zhan, J.; Lu, S.; Meng, Z.; Xiang, N.; Cao, Q.;
Se Pu. 26 (3), 301–5. 2008 May
traction/accelerated solvent extraction-gas chromatography-mass spectrometry Miao, M.
 References

Table of Contents

Introduction Technical Collateral:

Analytical Technologies Sample Preparation Methods
Glyceride Analysis Product Number Technique Title
AN 326 HPLC-UV Extraction of Drugs from Animal Feeds Using Accelerated Solvent Extraction (ASE)
Triglyceride Analysis
AN 335 HPLC-UV Accelerated Solvent Extraction (ASE) of Active Ingredients from Natural Products
Poly-Unsaturated Fatty Acids AN 356 IC-conductivity Determination of Perchlorate in Vegetation Samples Using Accelerated Solvent Extraction and Ion Chromatography
AN 357 HPLC Extraction of Phenolic Acids from Plant Tissue Using Accelerated Solvent Extraction (ASE)
Antioxidant Additives AN 363 HPLC Extraction of Herbal Marker Compounds Using Accelerated Solvent Extraction Compared to Traditional Pharmacopoeia Protocols

References
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