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Albumin liquicolor Quality Control

All control sera with albumin values determined by this method may be
Bromocresol green Method employed.
We recommend the use our HumaTrol quality control sera based on animal
Package Size serum or our SERODOS based on human serum.
[REF] 10560 1 x 1000 ml Complete test kit
156004 4 x 100 ml Complete test kit Automation
Proposals to apply the reagents on analysers are available on request. Each
[IVD]
laboratory has to validate the application in its own responsibility.
Method 1, 2
Notes
Bromocresol green forms with albumin in citrate buffer a coloured
1. The test is not influenced by bilirubin values up to 20 mg/dl. Each
complex. The absorbance of this complex is proportional to the albumin
100 mg/dl hemoglobin will represent an albumin increase of 0.1 g/dl, so
concentration in the sample.
cross hemolysis should be avoided.
Contents 2. Cross hemolysis and marked lipemia will interfere. A sample blank must
[RGT] 4 x 100 or 1 x 1000 ml Colour reagent (R1) be determined by pipetting 10 µl sample to 1000 µl physiological saline
Citrate buffer (pH 4.2) 30 mmol/l and measured against dist. water. The absorbance of the sample blank
Bromocresol green 260 µmol/l has to be subtracted from the absorbance of the sample.
3. The standard contains sodium azide. Do not swallow. Avoid contact with
skin and mucous membranes.
[STD] 1 x 3 ml Standard
Albumin see label Safety Notes
Sodium azide 0.095 %
[RGT]/R1 Warning
[STD] is standardised against the certified reference material CRM-
· Hazard statements
DA470k/IFCC.
H317 May cause an allergic skin reaction.
Reagent Preparation · Precautionary statements
[RGT]/R1 and [STD] are ready for use.
P234 Keep only in original packaging.
Reagent Stability P260 Do not breathe dust/fume/gas/mist/vapours/spray.
[RGT]/R1 and [STD] are stable up to the given expiry date when stored at 2- P262 Do not get in eyes, on skin, or on clothing.
25 °C. P281 Use personal protective equipment as required.
P303+P361+P353 IF ON SKIN (or hair): Take off immediately all
Contamination after opening must be avoided.
contaminated clothing. Rinse skin with water [or shower].
Specimen P305+P351+P338 IF IN EYES: Rinse cautiously with water for several
Serum, heparinised or EDTA-plasma minutes. Remove contact lenses, if present and easy to do. Continue
Stability in serum: at 2-8 °C up to 1 month rinsing.
P337+P313 If eye irritation persists: Get medical advice/attention.
at 15-25 °C up to 1 week
P401 Store in accordance with local/regional/national/international
Assay regulations.
Wavelength: Hg 546 nm, 578 nm P501 Dispose of contents/container in accordance with local/ regional/
national/international regulations.
Optical path: 1 cm
All patient specimens, calibrators and controls should be handled as
Temperature: 20-25 °C potentially infectious. All material of animal origin should still be treated as
Measurement: Against reagent blank. Only one reagent blank per series potentially infectious material.
is required.
References
Pipetting Scheme 1. Rodkey F. L., Clin. Chem. 10, 606 (1964)
HUMAN AUTOCAL, REF 13160, is recommended for the calibration of 2. Doumas B. T. et al., Clin. Chim. Acta 31, 87 (1971)
automatic analysers.
Pipette into cuvettes Reagent blank Sample or [STD] SU-ALBU INF 156001 GB 02-2024-21 |
Sample/ [STD] --- 10 µl
[RGT]/R1 1000 µl 1000 µl
Mix, incubate for 5 min. at 20-25 °C. Measure the absorbance of the
sample and the standard against the reagent blank within 30 min. (A).

Calculation of the Albumin Concentration

Conversion factor: 1 g/dl = 10 g/l

Asample
C = [STD] x ⎯⎯⎯⎯⎯⎯ g/dl; g/l]
A[STD]

Performance Characteristics
Linearity
The test is linear up to an albumin concentration of 6,5 g/dl or 65 g/l. Dilute
samples with a higher concentration 1 + 1 with physiological saline (0.9%).
Multiply the result by 2.
The albumin values in [STD] are traceable to AUTOCAL, which are traceable
to the reference material ERM-DA470k/IFCC.
Typical performance data can be found in the Verification Report,
accessible via:
www.human.de/data/gb/vr/su-albu.pdf or
www.human-de.com/data/gb/vr/su-albu.pdf

Normal Range in Serum or Plasma

3.8 - 5.1 g/dl or 38 - 51 g/l
Human Gesellschaft für Biochemica und Diagnostica mbH
Max-Planck-Ring 21 · 65205 Wiesbaden · Germany
Telefon +49 6122-9988-0 · Telefax +49 6122-9988-100 · e-Mail [email protected]