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Tel: 609-228-6898
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Red Nucleic Acid Gel Stain(10,000 ×)
1 Contents 2) Staining DNA after electrophoresis
a. Perform electrophoresis in an agarose or nondenaturing polyacrylamide
Cat.No. Product name Package gel without any stain.
b. Dilute the Red Stain stock reagent with 0.1 M NaCl at a ratio of 1:3300, for
HY-K1007 Red Nucleic Acid Gel Stain (10,000 ×) 500 μL
example: add 15 μL Red Stain stock reagent into 50 mL 0.1 M NaCl and
gently shake to form a staining solution.
2 General Information c. Cover the gel with staining solution in a suitable container such as a
polypropylene staining tray and incubate at room temperature for 30
Red Nucleic Acid Gel Stain (10,000 ×) (Red Stain) is a nucleic acid stain that
minutes with shaking. Staining time will vary depending on the thickness of
can be used as a safer alternative to the traditional ethidium bromide (EB)
the gel and the percentage of agarose or polyacrylamide.
stain for detecting nucleic acids in agarose gels or polyacrylamide gels.
d. Detect the bands under UV illuminator.
Compared to EB, Red Stain can’t penetrate the cell membrane into cells well
Note: Staining solution can be reused at least 2-3 times. Store staining
above the working concentrations used in gel staining. Red Stain has the
solution at room temperature protected from light.
same spectrum as EB and can be used exactly the same way in imaging
system. Additionally, Red Stain is higher in sensitivity than EB.
5 Storage
3 Features 4°C 1 year
Protect from light
1) Impenetrable to cell membrane.
2) High sensitivity: more sensitively than EB.
6 Precautions
3) High signal-to-noise ratio: strong fluorescence signal of the sample, low
background signal. 1) Since Red Stain is very sensitive, reduce the amount of DNA loaded by
4) Strong applicability: staining dsDNA, ssDNA or RNA. one-third to one-fifth.
5) Easy to use: similar to EB; no need to change the buffers or testing 2) TBE buffer has a higher buffering capacity than TAE, because
equipments. borate-containing reagents have better electrical conductivity.
3) Red Stain can be used to stain ssDNA and RNA, but it is twice as sensitive
4 Protocol as for dsDNA than for ssDNA or RNA.
4) Red Stain may irritate skin and eyes. Please wear gloves while handling.
Because of strong DNA binding affinity, Red Stain can be used to stain DNA
5) This product is for R&D use only, not for drug, household, or other uses.
before electrophoresis (pre-staining), as well as after electrophoresis
Please consult the Material Safety Data Sheet for information regarding
(post-staining).
hazards and safe handling practices.
1) Staining DNA before electrophoresis
a. Precast agarose gels with Red stain by diluting the Red Stain stock reagent
1:10,000 into the gel solution prior to pouring the gel.
b. When the gel is solid, load the samples and perform electrophoresis.
c. Detect the bands under UV illuminator.
Note:
a). The pre-staining protocol is not recommended for polyacrylamide gels.
MedChemExpress USA
Polyacrylamide gels can be stained using the post-stain protocol. Tel: 609-228-6898 Fax: 609-228-5909
b). Red Stain has good thermal stability, it can be added directly to hot E-mail:
[email protected]agarose solution. Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA
