Guru Nanak Institute of Pharmaceutical Sciences and Technology
Sub: Lab on Biochemistry Code: PT 297
Sl. No. - EXPERIMENT No.- 5 Date:
Aim: Detection of presence of ketose sugar by Seliwanoff’s’s test
Objective: 1. To detect the presence of ketohexoses in a given sample.
2. To distinguish ketoses from aldoses.
Pretest Question:
1. Glucose tests positive for Seliwanoff’s test. T/F
2. Aldoses may test positive for Seliwanoff’s test. T/F
3. When heated ketoses are more rapidly dehydrated than aldoses. T/F
4. Furfurals can be produced by tetroses and higher sugars. T/F
Principle:
It is a colour reaction specific for ketoses. One can distinguish aldoses from
ketoses based on their ability to form furfurals. When conc. HCl is added, ketoses
undergo dehydration to yield furfural derivatives more rapidly than aldoses.
These derivatives from complexes with resorcinol to yield deep red colour. The
test reagent causes the dehydration of ketohexoses to form 5-
hydroxymethylfurfural. 5-hydroxymethyfurfural reacts with resorcinol present in
the test reagent to produce a red product within two minutes. Aldohexoses reacts
so more slowly to form the same product within two minutes. Aldoses generally
exist in solution as pyranoses, whereas ketoses generally exist as furanoses, hence
the ability of ketoses to rapidly dehydrate to yield furfurals.
Guru Nanak Institute of Pharmaceutical Sciences and Technology
Sub: Lab on Biochemistry Code: PT 297
Reagents:
1. Seliwanoff’s reagent- 0.05% (w/v) resorcinol in 3 (N) HCl
2. 1% solution of different carbohydrates (Glucose, Fructose, Lactose,
Maltose, Sucrose, Starch)
Procedure:
2ml of Seliwanoff’s reagent was taken in a test tube and 0.5ml of test solution
was added to this. Test tube was place in boiling water bath. Test was performed
with different carbohydrates and with water as blank. A cherry red condensation
product will be observed indicating the presence ketoses in the test sample. There
will be no significant change in colour produced for aldose sugar.
Observation Table:
SL. Sample Initial observation Final observation Interpretatio
No. n
1% Glucose
1% Fructose
1% Lactose
1% Maltose
1% Sucrose
1% Starch
Guru Nanak Institute of Pharmaceutical Sciences and Technology
Sub: Lab on Biochemistry Code: PT 297
Results & Conclusions:
Critical thinking questions:
1. What is the difference between ketose & aldose?
2. Which acid is being used in Seliwanoff’s test & why?
3. Why fructose tests positive in Seliwanoff’s test?
4. What are the similarities and dissimilarities between Molisch’s test &
Seliwanoff’s test?
Remarks:
Attendence(10) Viva(1 Performance(20) Total(40)
0)
Cleanliness & Conduct & Methode Critical thinking
manual involvement(4) Accuracy(4) question(8)
maintainance(4)
Signature of the teacher
