Experiment No. 2 Osmotic Behavior of Potato Slices
Experiment No. 2 Osmotic Behavior of Potato Slices
Experiment No. 2
INTRODUCTION:
The process of moving water across a semi-permeable membrane from a low
concentrated solution to a high concentrated solution is called osmosis. A semi-
permeable membrane is a thin sheet of tissue or layer of cells acting as a wall that allows
only some molecules to pass through.
In plants, water enters the roots by osmosis. The plants have a higher
concentration of solutes in their roots than in the soil. This causes water to move into the
roots. The water then travels up the roots to the rest of the plant. Osmosis works in both
directions. If you put a plant into water with a higher salt concentration than the
concentration inside its cells, water will move out of the plant. If this happens then the
plant shrinks and will eventually die.
Potatoes are a great way to demonstrate the process of osmosis. As you are
conduct the experiment you will discover which potato pieces will expand and shrink in
size as the water moves from a low concentration to a high concentration.
OBJECTIVES:
Through conducting the experiment, you should be able to:
1. Observe the process of osmosis
2. Predict the effects of isotonic, hypotonic and hypertonic environments on osmosis
in potato
MATERIALS:
3 beakers Salt
Distilled Water Stirring Rod
Pocari Sweat Tablespoon
Potato
PROCEDURE:
1. Peel and then cut your potato into three equal pieces.
2. Measure the length of the three pieces of potato and take note of your
observations on the table below.
3. Fill your two beakers with 100 ml distilled water.
4. Mix 3 spoonful of salt into one of the beakers and stir.
5. Fill one beaker with 100 ml pocari sweat.
6. Label each beaker with the respective solutions.
7. Place three pieces of potato into each beaker and wait.
8. Compare the potatoes after 15 minutes, 30 minutes, 45 minutes and 60 minutes
then write your observations.
Data Gathered:
Before After 15 After 30 After 45 After 60
(cm) minutes minutes minutes minutes
Potato in
Hypotonic
Solution
Potato in
Hypertonic
Solution
Potato in
Isotonic
Solution
QUESTIONS:
1. What happened to the potato piece in the hypertonic solution? hypotonic solution?
isotonic solution?
2. How a potato can demonstrate the process of osmosis?
CONCLUSION:
Why is osmosis important to the cell?