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Anaerobic Jar Methods and Principles

The document describes various methods for creating anaerobic conditions, including the McIntosh-Fildes jar, candle jar, and gas pack system. Each method utilizes different principles and materials, such as palladium catalysts and chemical reactions, to displace oxygen and support the growth of anaerobic bacteria. The gas pack system is noted for its simplicity and commercial availability, although it is considered expensive.

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51 views4 pages

Anaerobic Jar Methods and Principles

The document describes various methods for creating anaerobic conditions, including the McIntosh-Fildes jar, candle jar, and gas pack system. Each method utilizes different principles and materials, such as palladium catalysts and chemical reactions, to displace oxygen and support the growth of anaerobic bacteria. The gas pack system is noted for its simplicity and commercial availability, although it is considered expensive.

Uploaded by

sre46567
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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a r e Jaw iAlfa

McINTOSH -FILDE'S JAR


Principle
Dsplacement of Oagas with Hz gas and removal of Oh gas by combining
with Ha gas to form water in prescnce at palladium catalyst

Structure
. t consist of stout glass or metal ja
2 Lud is provided with clamp terminals for clectric supply, twO gas tight
valves one for inlet and other for outlet of gases.
3, A groove underside of lid for attachment of catalyst.

Catalyst:
Palladiumized asbestos or palladium coated alluminium pellets,

Method
are kept in to the jar. After
Inoculated plates
and
creating vacuum, mixture of
in
nitrogen,hydrogen Lid
carbon dioxide gases are introduced the jar.
clamped tightly with attached catalyst. Ha gas combines with reduced O gas in
presence of catalyst.

Indicators

Used to verify the anaerobic conditions in the jar.

Reduced methylene blue - colorless changes to blue on exposure to O

gas.
Pscudomonas aeruginosa- negative control.
honor8xdum tetani -positivecontrol
CANDLE JAR

Displacements
of O; with CO
Principle:
gas.

with lid is taken. A


Method: A glass jar inoculated
candle is kept along with
lighted sealed
inside the jar. Lid is
culture plates Vaseline.
with the s m e a r of
immediately
to use all Oxygen
candle is assumed
Burning
it is extinguished. CO
inside before supports the
raised, which
concentration is
PneumococC1.
bacteria e.g.
growth of many
ineffective and incomplete
It is an
m e t h o r anaerobios1s.
met A
honor 8X
GAS PACK SYSTEM

combining it with hydrogen


Principle: Removal of oxygenofby
cold catalyst.
water in the presence
to form
1. Commercially available
foils packet containing
tablets of three chemicals, sodium borohydrate,
citric acid and sodium bicarbonate.

2. Water is added to the packet just before use.

3. Packet is kept in an anaerobic jar, immediately made air


tight by clamps and screw.

4. hydrogen and carbon dioxide gases are generated by the


chemical reaction in presence of catalyst.

5. H2 absorbs 02 of inside the jar. It is a simple method


commercially available.

Gas pack is expensive

honor 8X
GASPACK SYSTEM
Principle: Removal of oxygen by combining it with hydrogen
to form water in the presence of cold catalyst.

1.Commercially available foils packet containing


tablets of three chemicals, sodium borohydrate,
citric acid and sodium bicarbonate.

2.Water is added to the packet just before use.

3. Packet is kept in an anaerobic jar, immediately made air


tight by clamps and screw.

4. hydrogen and carbon dioxide gases are generated by the


chemical reaction in presence of catalyst.

5. H2 absorbs 02 of inside the jar. It is a simple method


commercially available.

Gas pack is expensive

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