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DNA Bird Sexing - Brochure

The Institute of Biochemistry and Biotechnology at the University of Veterinary & Animal Sciences has developed a reliable method for avian DNA sexing using molecular markers, achieving 99.9% accuracy. The process involves PCR analysis of DNA from the sex chromosomes, distinguishing between male (ZZ) and female (ZW) birds based on gene size differences. Instructions for blood sample collection and a molecular test request form are provided for those seeking to utilize this service.

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19 views3 pages

DNA Bird Sexing - Brochure

The Institute of Biochemistry and Biotechnology at the University of Veterinary & Animal Sciences has developed a reliable method for avian DNA sexing using molecular markers, achieving 99.9% accuracy. The process involves PCR analysis of DNA from the sex chromosomes, distinguishing between male (ZZ) and female (ZW) birds based on gene size differences. Instructions for blood sample collection and a molecular test request form are provided for those seeking to utilize this service.

Uploaded by

sayemkhanmarwat
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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University of Veterinary & Animal Sciences, Lahore-Pakistan

Institute of Biochemistry and Biotechnology

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Avian DNA Sexing

In the past, phenotypic identification of sex was very


difficult in various species of birds causing problems in pairing for
breeders and bird lovers. IBBT scientists took this challenge and
after an extensive lab work developed molecular markers that
could detect gender using DNA information with 99.9% reliability.
DNA sexing uses the process PCR to analyze the DNA from the
sex chromosomes of each bird. Bird gender can be determined by
the sex chromosomes. Females have two different sex
chromosomes (called ZW) while males have two of the same sex
chromosomes (called ZZ). The gene CHD is found on both the Z
and W chromosomes but the size of the gene on the W
chromosome is smaller than the gene on the Z chromosome. The
PCR process essentially duplicates the CHD gene thousands of
times until there is so much DNA that they can actually be
photographed on a special instrument. Since the sizes of the two
genes are different, males and females are easily identified. A
female will have a larger Z-size gene and a smaller size W gene.
Males will have only the Z gene.

Institute of Biochemistry and Biotechnology University of Verterinary and Animal Sciences Lahore
University of Veterinary & Animal Sciences, Lahore-Pakistan
Institute of Biochemistry and Biotechnology

• Hold the bird comfortably so that you can access the


bird’s toe.
• Remove the new sterile needle and prick the bird’s
toe just above the claw, after a few seconds a visible
drop of blood will appear.

• Collect the blood in collecting tube.

• Complete the form given below with all the requested


information regarding the birds that are to be sexed.

• Post the bags/ envelope having blood sample to us.

Institute of Biochemistry and Biotechnology University of Verterinary and Animal Sciences Lahore
University of Veterinary & Animal Sciences, Lahore-Pakistan
Institute of Biochemistry and Biotechnology

OWNERS INFORMATION ( Please complete all sections)


DATE: OWNERS NAME:

VET PRACTICE/VETERINARIAN:
Address:
Post Code:
Tel: Fax:
Email:

MOLECULAR TEST REQUEST FORM

Please print clearly Please indicate the test you require under TEST CODE and 9 if you require a certificate (optional)
MDS REF No. YOUR REFERENCE (Sample details) TEST
CERT. COST
(Office use only) Breed / Species (Must be completed) Name/Ring No. CODE
9

TOTAL

Contact Detail:
Dr. Ali Raza Awan
Assistant Professor, Institute of Biochemistry & Biotechnology
University of Veterinary & Animal Sciences, Lahore
Phone: +92-42-99211449-50 Ext: 302
Email: [email protected]

Institute of Biochemistry and Biotechnology University of Verterinary and Animal Sciences Lahore

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