JIWAM UNIVERSITY
GWALIOR (M.P.)
SYLLABUS
FOR
M.Sc. (BOTANY)
SEMESTER SYSTEM
SESSION
2024 — 2026
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BOT 101 | Bacteriology. Virology & General Microbiology (00 Marks)
BOT 102 | Biology and Diversity of Fungi and Plant Patholog, (100 Marks)
BOT 103 | Biology and Diversity of Algae, Bryophytes and Lichens | (100 Marks)
BOT 104 | Biology and Diversity of Pteridophytes and Gymnosperms [ (100 Marks)
BOT 105_| Practical Course Based on Theory Course 101 & 102 {00 Marks) |
BOT 106 | Practical Course Based on Theory Course 103 & 104 | (100 Marks) |
MSe. Il SEMESTER
[BOT 201 | Eeology-1 Climatology. S nd Autecol
jence [ (100 Marks)
BOT 202 | Angiosperm Anatomy. Embryology and Palynolog [100 Marks)
[BOT 203 | Water Relations. Growth and Development [C00 Marks)
| BOT 204 Plant Biochemistry and Metabolism, | (100 Marks)
BOT 205__ Practical Course Based on Theory Course 201 & 202 [C100 Marks)
BOT 206 | Practical Course Based on Theory Course 203 & 204 [C100 Marks)
M.Sc. IT SEMESTER
[BOT 301 | Angiosperm Morphology and Taxonomy (100 Marks)
‘BOT 302 | Cytology and Molecular Biology of Plants (100 Marks)
BOT 303 _| Biometry. Bioinformatics and Instrumentation (100 Marks)
[BOT 304 | Ecology-II Synecology. Ecosystematology & Phytogeography (100 Marks)
| BOT 305 | Practical Course Based on Theory Course 301 € 302 (100 Marks)
BOT 306 | Practical Course Based on Theory Course 303 & 304 (100 Marks)
M.Sc. IV SEMESTER
BOT 401 | Genetics, Plant Breeding and Evolution (OO Marks)
BOT 402 | Plant Biotechnology: In Vitro Culture, Genetic Engineering and IPR | (100 Marks)
Issue
BOT 403 | ELECTIVE PAPERS (OPTIONAL) ~ (Any two)
BOT E01 | Industrial Microbiology (100 Marks)
BOT E02 | Biochemistry and Metabolism in Plants _ (100 Marks)
BOT E03 | Ethnobotany and Isolation of Natural Products (100 Marks)
BOT £04 | Stress Physiology | (100 Marks
BOT £05 | Plant Pathology and Mycotoxicolozy (100 Marks
BOT E06 | Agro ecosystem (100 Marks)
BOT 404 | Practical Course Based on Theory Course 401 & 402 (100 Marks
BOT 405 | Practical Course Based on Theory Course from E110 F6 (any two) | (100 Marks)
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@ scanned with OKEN ScannerM Sc. Botany 2024-2026 (For Colleges only)
BOT 101: BACTERIOLOGY, VIROLOGY & GENERAL MICROBIOLOGY
UNIT 1:
Bacterial taxonomy;
Identification of bacteria,
General characters of Rickettsia and Chlamydia.
Diseases caused by Rickettsia and Chlamydia
Mode of nutrition in bacteria; autotrophy, heterotrophy
UNIT IT
General account of sterilization culture media, pure culture techniques;
A general idea about bacterial toxins and enzymes;
Bacteriophage:
Bacterial diseases: caused by Escherichia coli, Shigella.
UNIT TL
General properties and evolution of viruses;
Cultivation of virus and viral assay;
Transmission of plant viruses and control measures.
Oncogeneic viruses and tumorogenesi
Viral diseases: Encephalitis, Hepatitis AIDS and Rabies.
UNITIV
Biological nitrogen fixation: symbiotic and non symbiotic nitrogen fix
Fermentation technology: principle and types of fermentation,
‘Microbial degradation of pesticides and hydrocarbons.
‘Mycoplasma: general account and important diseases caused by them.
UNITV
Microbial conversion of waste product with particular reference to alcohol and biogas
General account of Immunity, properties of antigens and antibos
Allergy and types of allergies.
Mycotoxins and their harmful effects.
PRACTICALS 101
ymbiosis,
1. Preparation of culture media.
2. Isolation of Bacillus and Rhizobium spp form soil and nodules.
3. Various methods of bacterial staining to study cell wall, endospore, capsule ella,
4. Identification of important genera by using biochemical tests: Escher wobacter,
Staphylococcus, Bacillus, Pseudomonas, Rhizobium, Streptomyces, Nanthomonas.
5. Construction of bacterial growth curve.
6. Quantitative estimation of bacteria in milk.
7. Isolation of streptomycin — resistant mutants of bacteria,
8. Sensitivity test of bacteria using different antibiotic:
9. Purification of TMV and study of thermal inactivation point and dilution point,
10. Virus concentration determination by local lesion on host.
11. Study of common vectors of plant virus: Nematodes, fungi and insects.
12, Bacteriophage isolation
13, Isolation and enumeration of bacteria, actinomycetes
different techniques.
14, Use of selective media for isolating micro- organisms.
15, Fermentation of alcohol and biogas from waste materials (Demonstration)
and fungi from soil, thizosphere and seed using
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ANT PATHOLOGY
BOT 102: BIOLOGY AND DIVERSELY OF FUNGE AND T
UNITL
Recent trends on the elassitioation of fingi with reference to morphological and
paramorphologieal etiteria:
Comparative study of following subativision;
Mastigomyeotina: d2dugo.Peronaypone, Plamopont
uNiTn
Comparative staty of tatloving subs
Zyyomyootina: Macon Naizypus, Nvrcephalasirum
Ascomyootina: Yarnina, Boterivella, Penicillium, Chactomium, Morchella
sions
unit
Comparative study of following sub-division:
widiomycotina: Paccinia, Melampsona, Ustilag
Deuteromyeotina: Fusarium, Cersaspana, Colletotr
Mushroom cultivation: Mycorrhizal application in agriculture and forestry;
Fungal cytology and genetics: Heterothallism, heteroka :, parasexual cycle, mutation,
Polyporus, Cvathus
un,
UNITIV
Symptomatology in fungal, bacterial and vital infection of plants
Exiology and control of the following erop diseases
1. Paddy: paddy blast, paddy blight
2. Wheat: Black stem rust, Bunt of wheat
3. Bajara: green eat and Ergot
4. Sugarcane: Red rot disease of sugarcane,
$. Ground nut: Tikka diseas
6 Maize Smut
UNITY
Role of enzymes and toxins in pathogenesis;
Disease control by physical, cliemical and biological methods,
Crop rotation, plant quarantines, seed certification
PRACTICAL 102:
Study of the m
theory. Study of sympto
{in culture) vitamin requirement, staining techniques, induction
Study of diseased specimens of plants with reference to symp
Isolation, purification and single spore culture of pathogens.
Measurement of the activity of enzymes of fungal pathogens: Cellulose, Pectinases.
Laboratory testing of fungicides (systemic and non-systemic) against pathogenic fungi
Demonstration of biological control of pathogenic fungi in vine
phological characters and reproductive structures of the genera mentioned in the
aitology of diseased species. Carbon and nitrogen utilization by fungi
mu isolation of mutants.
Kolo,
2
3
4
5
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BOT 103: BIOLOGY AND DIVERSITY OF ALGAE, BRYOPIYTES AND LICHENS
UNITI
Comparative survey of important systems of cl
Criteria for algal classification and modern trends
Diagnostic features of algal phyla, tange of thallus and reproductive diversity; life history patterns,
parallelism and evolution,
UNIT IT
Comparative account of algal pigments ; light microscopic structure, ultra structure and function of
cell wall, flagella, chloroplast, pyrenoids and eyespots and their importance in taxonomy.
Study of Cyanophyta, Chlorophyta, Xanthophyta, Bacillariophyta, Phacophyta and Rhodophyta up to
the order level with reference to the following genera: Anabaena, Gonium, Chlorella, Enteromorpha,
Bulbochaete, Clostridium, Acetabularia, Nitella, Botrydium, Navicula, Cyclotella, Batrachospermum
and Gracilaria
UNIT IIT
General characteristics of the division: Diophyta, Chrysophyta and Cryptophyta.
Distribution of algae in soil, fresh water and marine environment, role of algae in soil fertility,
productivity in fresh water and marine environment algae role in fisheries, algae in symbiotic
association, algae in polluted habitats, algae as indicator of pollution, fossil algae, algae in
biotechnolo:
UNITIV
Origin of Bryophytes: Primitive vs. advanced characters, derived features: evolutionary lines.
Classification.
Comparative morphological, anatomical and cytological studies of gametophyte and sporophytes of
Calobryales, Jungermanniales, Sphaerocarpales, Marchantiales, Takakiales, Sphagnales, Andreales
and Bryeles.
UNITV
Experimental studies in Bryophytes
Spore germination, Protonemal differentiation, bud formation
Parthenogenesis, apogamy, apospory and regeneration.
Bryogeographical regions of India with reference to central India,
Lichens: General account, structure and reproduction
fication of algae:
PRACTICAL 103:
1. Collection and study of algae mentioned in theory, identifi
monographs.
2. Preparation of synthetic medium and cultivation of algae, unialgal and axenic culture and their
maintenance.
3. Collection, preservation of algal herbarium (10 specimens).
4, Preparation of pigments.
5. Staining techniques of cytology studies.
6
7.
ion up to generic level using algal
Study of electron microscopy of some algae.
Morphology and structural study of representative member of the following group using cleared whole
amount preparation, dissection and section: Jungermanniales ~ ellia and Porella (or any other lealy
liverwort); Marchantiales-Plagiochasma, Dumortiera, Fimbriaria, (dstralla, Reboulia, Targionia,
Conocephalum/ Weisnerella, Sphagnales/ Sphagnum! Bryales
Experiments to study spore germination, formation of protonema and bud d
Study of Bryophytes in their natural habitats i
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BOT 104: BIOLOGY AND DIVERSITY OF PTERIDOPHYTES AND GYMNOSPERMS
UNITI
Evolution of Pteridophytes; Soral and stealer evolution
Classification of Pteridophytes
UNIT
Comparative organography. systematics; reproduction and phylogeny of the following:
Psilophytales, Rhyniales, Zosterophyllophytales.
Psilotales.
Lycopdiales, L
‘Sphenophytlales
Ophioglossales, Marattiales, Osmundales, Filicales, Marsileates, Salviniales.
idodendrales
UNIT II
‘Speciation and evolutionary trends in ferns;
Cytology
Polyploidy and hybridization;
Preridophytic life — cycle, apospory, vegetative apomixes,
Recent trends in the classification of Gymnosperms
UNITIV
Morphology and anatomy of vegetative and reproductive organs, fossil representatives and
interrelationship of Cycadales, Ginkgoales, Coniferales, Taxales, Ephedrales, Welwitschiales and
Gnetales.
UNITV
Structure and evolution of archegonium in Bryophytes, Pteridophytes and Gymnosperms
Distribution of living and fossil Gymnosperm in India.
Economic importance of Gymnosperms.
PRACTICALS 104
1. Study of morphology and anatomy of vegetative and reproductive tissues and organs using cleared
whole mounts, dissections, sections, macerations and permanent preparations of living and fossil
forms covered under theory.
Experiments on spore germination of prothallus, induction of sporophytes.
Preparation of models (Plasticine! thermocol) to demonstrate stealer evolution.
Study of Pteridophytes in their natural habitats
Comparative study of the anatomy of vege
Picea, Cupressus, Cryptomeria, Taxodium, Podocarpus, Cephalotaxus,
Ephedra and Gnetum.
6. Study of the important reproductive stages through speci
7. Preparation of models (Plasticine! thermocol) (0 demo
microsporangia of Cycas, Pinus, Taxus, Ephedra, Gnetum. See
Pinus, embryo of Pinus.
ce and reproductive parts of Ginkgo, Cedrus,
aucaria, Agathis
BAL
Taxus,
ind permanent slides.
‘ale the position and structure of
cale complex in female cone of
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ELECTIVE PAPERS (OPTIONAL)
INDUSTRIAL MICROBIOLOGY
poT EO!
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Development and scope of
Oner fa seape of a Microbiology. Use of Fermentation equipments: Design and
iene dearer te nd Continuous fermenters. Computer control of fermentation
is of fermentation media, Raw materials (substrates).
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Use of microorganisms in industries through
Strategies for isolation and screening of industrially important microorganism.
iprovement of industrially important microbial strains.
uNIT HIT
Industrial product of vinegar.
Industrial product of citric acid,
Industrial product of antibiotics; penicillin and streptomycin,
Industrial product of amino acids; glutamic acid and lysine.
UNITIV
Microbes as a source of Single Cell protein (SCP).
Mushrooms and food value of mushrooms.
Dairy product from microorganisms; butter, yogurt and cheese
Hygine and safety in fermentation industries
UNITV
Biopesticides: bacterial, fungal and viral control of insect pests.
Biofertilizer: production and method of application.
Bioremediation.
PRACTICALS E01:
1. Isolation and ide
2. Inoculation of fungi
the different types ©
‘ast and fungi from bakeries and fermenters of
ierose solutions and identifi
during different incubation periods.
ntification of bacteria, ye
‘and bacteria on steril
f amino acids and organic
lized glucose and su
acids in filtrate
curd, rotten fruits and
thromatograph: ; cf one
3, ne a PY) cation of different types of fungi and bacteria from
4 reeetabies ¢ ifferent types of mushrooms from focal arew region: inventory and analysis of their
. Collection of
ts. (Chromatography)
tents. tivation of ei
im of three antibiotics a
$ vsti YW, yay pu
le mushrooms,
amino acid cor
ainst common plant pathogens in Petri plates
5. Preparation of spawn for cut
6 Observation of the antagon's
(dise methods). aw
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joT £02: BIOCHEMISTRY AND ME
ont ;
Lipid: General structure, e
Synthesis of fatty acid,
B-Oxidation
Synthesis of carbohydrates from ff
Protein: Amino acid frauen ir
‘TABOLISM IN PLANTS
lassification,
y acids,
and Biosynthesi formati
Biosynthesis; protein conformation, protein synthesis.
ales ieneral structure of important i i
7 at ortant phenoli gr in
shikimie acid pathway and phenolic compound synthesis ene
Geer ssroctre and synthesis of alkaloids from amino acids.
-gulators: structure and biosynthesis of Ethylene, Jasmonates and Brassinolides.
UNTO
Cell wall components.
Cellulose: structure and model for biosynthesis
plasma membrane levi
structure and plants.
‘anogenesis: a general account of cyanogenic compounds as glycosides.
structure and operation of the model for synthesis at
UNTIV
DNA and RNA: structure
DNA replication.
Transcription in prokaryotes and eukaryotes. transcript modification.
Regulation of translation.
UNITV
Nitrate metabolism in plants.
Nitrogen fixation, Nitrogenase system.
‘Ammonium assimilation.
GS-GOGAT system.
PRACTICALS: 204
1. Estimation of total nitrogen by Wilda method.
2. Estimation of titrable and total acidity. El
3 Estimation ‘of seed germination as ‘affected by red and Infrared radiatior
5. Radioisotops ;
ciples of counters
tation and pri
Poncicn mn of stare.
6. Extraction and estimat! aan
7. Determination of reducing: SOE op vests)
E. Tdenuication of different kinds oF
9, Estimation of amin acids 2 urconaning amino acids with aromatic ring (sp tes
10. Identification of proline, SP an
11, Determination of Isoelect™ ane prepress: .
3e ans ft Amylase oF phosphorylase =) \ ¢
\v
ON oi 4
13, Extraction of amylas eal
1a, Determination of Km and V"=X © 4 ie
a oS ae
af ow ARE
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(Til
UNTIV
UNITV
PRACTICAL E03:
M.Sc. Botany 2024-2026 (For Colleges only)
: ETHNO y
or BOs: BOTANY AND ISOLATION OF NATURAL PRODUCTS
Ethnobotany. its scope, interdiscipli
interdisciplinary approa
Ethnic groups of India : major and minor tb ea
biodiversity, taboos and totems.
World centers of Ethnobotany with special reference to India.
styles of ethnic tribes, conservation practices of
ole of Ethnobotan; ic 4
Sieh tnd principles phic prints, health care and development of cottage industries in India
weeneral idea of Save mie f Homeopathy. Allopathy, Unani and Siddha system of medicines:
alas les of plants 7 i
A cSiines in different systems. plants and plant parts their extraction and preparation of
gents.
Scope and uses of essential oil from plants as perfumes, cosmeties and as flavorin|
1g Lemon grass.
Preparation of perfumes from aromatic plants with special reference to the followin;
Palm-rosa, Mint, Lavender, Rose, Eucalyptus and Vetiver.
Plants used in medicine with special reference to following.
‘tdhatoda vasica, Asparagus racemosus, Hollarhina antidysenterica, Tinosport cordifolia
Torminalia arjuna, Terminalia bellerica, Terminalia chebula, Prerocarpus ‘marsupium, Commiphora
wight.
Regional relevance and credibility of medicinal plans used by tribals of M. P-
Vlans used in medicine with special reference to following
‘Argemone mexicana, Boerhaavia diffusa, Eclipta prostrata,
Tylophora indica, Rauwolfia serpentina, ‘Dioscorea deltoids. : :
Plants used in scarcity, emergency and as supplementary foods by tribals of India.
Psoralia coralifolia, Withania sominifera.
ibals.
4 study of plant material used tribal :
aren ito of ethno botanical importance.
1. Visit to tribal pl
‘scription of important plant
2. Identification and de lants of
3. Mentification of important aromallc plants of the locality.
4. Extraction of active ingredients of pla and plant parts.
5. Extraction of perfumes of arom ie MS cd 7
6 Pharmacognostie method of identi OF AMY
7. Methods of preparation ° Kant Ae locality.
Hara sam a ina igs used y tribal fr household purpose.
lentification an‘ :
wer hotteD pwn
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jor 04 STRESS PHYSIOLOGY
itl ee A
0 prs ad water: Chemica an water oni i
Determ of water tential gradients,
Potential of plants and sissies. by Chardakov's, pressure chamber and
psychometric methods,
Siomatal size, frequency and measureme
opening and closing, ‘
: ological iples of dry land farming.
in icient, w: ici
t et water use efficiency, stress - degree - day c
thee relationship to several plant physiological processes, Py concent gaa
“availability of soil water and determination of soil water potential
nts of stomatal aperture, porometry, Mechanism of stomatal
stress index and
UNIT cand d
ight and drou: i
Drougl ight tolerance mechanisms: drought escape, drought tolerance with high tissue water
rental; rought tolerance with low tissue water potential
Morphological, physiological and biochemic: a
og al parametet
Screening methods to study drought resistance ata
wT
“Antitranspirants : Different types, mode of action and their use in alleviation
Nitrogen fixation and drought.
Ultra structural consequences of drought
of water stress.
UNTIV
‘C