Automated Hematology Analyzer

XN-L series

XN-550/XN-450
/XN-350
Troubleshooting

Read this manual when you encounter a problem, and to perform instrument maintenance.
The explanations in this manual assume that you have already read "General Information".

The following manuals are provided as Instructions for Use:

• General Information
• Basic Operation
• Troubleshooting

Code No. CF904657 en-eu

PRINTED IN JAPAN
Date of Last Revision: 04/2020
© SYSMEX CORPORATION 2014-2020 Software Version: Ver. 5 onwards
 Revision History

Revision History

07/2014
Initial issue
Software version: 00-00

10/2014
Software version: 00-04

12/2014
Software version: Ver. 3

06/2015
Software version: Ver. 3

08/2015
Software version: Ver. 4

03/2016
Software version: Ver. 5

06/2016
Software version: Ver. 5

02/2017
Software version: Ver. 5

05/2017
Software version: Ver. 5

01/2018
Software version: Ver. 5

08/2018
Software version: Ver. 5

06/2019
Software version: Ver. 5

04/2020
Software version: Ver. 5
Updated manuals: Basic Operation, General Infomation

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Table of Contents

Revision History 3

Chapter 1 Troubleshooting 7
1.1 Error message list (in alphabetical order) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 [Help] dialog box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
1.3 Causes of errors and remedial actions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Chapter 2 Maintenance 69
2.1 List of maintenance tasks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
2.2 Displaying maintenance screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
2.3 Maintenance screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
2.4 Executing routine cleaning (XN-550) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
2.5 Executing routine cleaning (XN-450) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
2.6 Executing routine cleaning (XN-350) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
2.7 Replenishing the reagent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
2.8 Replacing the waste container . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
2.9 Testing instrument operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
2.10 Checking the cycle count (counter) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
2.11 Checking the instrument status (sensor) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
2.12 Replacing the reagent. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
2.13 Replacing the piercer (XN-550/XN-450) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
2.14 Replacing the air pump. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
2.15 Replacing the fuse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
2.16 Cleaning the aspiration unit tray (XN-550/XN-450) . . . . . . . . . . . . . . . . . . . . . 94
2.17 Cleaning the aspiration unit tray (XN-350) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
2.18 Adjusting the monitor image quality (XN-550) . . . . . . . . . . . . . . . . . . . . . . . . . 98
2.19 Maintenance and inspection checklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

Chapter 3 Calibration 101

3.1 Overview of calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
3.2 Performing calibrator calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
3.3 Performing precision check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
3.4 Checking the calibration log/precision check log . . . . . . . . . . . . . . . . . . . . . . 113
3.5 [Calibration History] screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
3.6 [Precision Check History] screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119

Chapter 4 Checking Logs 121

4.1 Logs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
4.2 Log screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124

Chapter 5 IP Messages 129

5.1 Overview of IP messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129
5.2 List of IP messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133
5.3 IP message judgment conditions and judgment methods . . . . . . . . . . . . . . . 135

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Index 137

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Chapter 1 Troubleshooting

1.1 Error message list (in alphabetical order)

The following is an alphabetical list of error messages.

Note:
In the situations below, remove the sample tubes from the instrument and turn OFF the power.
• An error message marked with ☎ (phone sign) appears in the error message list.
• An error persists after the indicated action is taken.
Instrument repair or replacement of parts is necessary. Contact your Sysmex service representative.

Error message Action

Numeric -0.03 MPa pressure error  13

0.06 MPa pressure error  12
A Abnormal pressure loss  12
Analysis item not specified  30
Analysis result is high  46

☎ Aspiration Sensor error  29

Aspiration unit front-back motor error  25
Aspiration unit up-down motor error  25
B Background check error  33
Barcode reader communication error  31
Blood cannot be aspirated.  26
Bubbles in RBC detector  40
C Cannot recognize Fluorocell RET information  63
Cannot recognize Fluorocell WDF information  63
Cannot recognize sampler adapter  67
CELLPACK DCL has expired  57
CELLPACK DFL has expired  57
Check Measurement Mode  33
Control has expired.  32
Control is not entered.  32
D Data Errors  46
E Environment temperature is high  15
Environment temperature is low  15

☎ Environment temperature thermistor error  15

Execute Routine Cleaning  51

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Error message Action

Execute Routine Cleaning (warning)  51

F Failed to acquire items.  30
Failed to read sample number.  30

☎ FCM cover is open.  50

FCM reaction chamber temperature is high  15
FCM reaction chamber temperature is low  15

☎ FCM reaction chamber thermistor error  15

FCM sheath aspiration error  19

☎ FCM sheath thermistor error  15

Fluorocell RET aspiration error  19
Fluorocell RET has already been used  61
Fluorocell RET has expired  58
Fluorocell RET is not installed  60
Fluorocell RET RFID tag error  64
Fluorocell WDF aspiration error  19
Fluorocell WDF has already been used  61
Fluorocell WDF has expired  58
Fluorocell WDF is not installed  60
Fluorocell WDF RFID tag error  64
H Hand F-B motor error  67
Hand L-R motor error  67
Hand open/close error  67
Hand up-down error  67
HGB error  42
I Insufficient blood volume (short sample)  26

☎ Internal Error  32
Invalid analysis item is specified  31
Invalid analysis item is specified (sampler analysis)  31
L L-J Control Error  32

☎ Laser life  31

☎ Laser output error  31

Low count error  47
Lysercell WDF has expired  57
M Mixing error  67
O Out of CELLPACK DCL  16

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Error message Action

Out of CELLPACK DFL  16

Out of diluted CELLPACK DST  24
Out of Fluorocell RET  17
Out of Fluorocell WDF  17
Out of Lysercell WDF  16
Out of SULFOLYSER  16
P Piercer replacement is required.  51
PLT channel error  43
PLT sampling error  34
Press Start SW  60

☎ Pressure Sensor Error  14

Q QC not executed.  33
R RBC channel error  43
RBC detector clog  40
RBC sampling error  34
RBC sheath fluid aspiration error  19
RBC/HGB chamber not draining  22
Reagent heater temperature is high  15
Reagent heater temperature is low  15

☎ Reagent heater thermistor error  15

Replace air pump.  54
RETchamb not draining  23
RET channel error  43
RET sampling error  37
RET Scattergram sensitivity error  33
RFID communication error  32
Right cover is open  50
Right cover is opened  50
Rinse cup pinch valve move error  23
S Sample number not input  30
Sampler Adapter holder is open.  66
Sampler Adapter holder is opened.  67
Sampler adapter is not placed  67
Sampler cover (front) is open.  66
Sampler cover (front) is opened.  66

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Error message Action

Sampler cover (manual unit) is open.  66

Sampler cover (manual unit) is opened.  66
Sheath motor error  25
SULFOLYSER has expired  57
T ☎ Temperature stabilizing error  15
The dye holder is open.  50
The tube holder is opened.  50
Tube holder motor move error  25
Tube remains in tube holder  30
Two tubes are in tube holder  30
V ☎ Vacuum Sensor Error  14
W Waste chamber 1 not draining  20
Waste chamber1 pinch valve move error  23
Waste chamber 2 not draining  20
Waste chamber2 pinch valve move error  23
Waste container is full  23

☎ Water leak detected  24

☎ Water leak detected (analysis not possible)  24

☎ Water leak sensor error  24

WB aspiration motor error  25
WBCchamb not draining  23
WDF channel error  43
WDF sampling error  37
WDF Scattergram sensitivity error  33
Wrong reagent installed in Fluorocell RET holder  60
Wrong reagent installed in Fluorocell WDF holder  60
X X-bar control error  32
X-barM control error  32

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1.2 [Help] dialog box

When an error occurs, the [Help] dialog box appears.
If an error occurs while another dialog box is displayed, [Error] will appear in the control menu at the bottom of the
screen.
Close the dialog box that appear, and touch [Error]. The [Help] dialog box appears.
You can check details on the error and what action to take in the [Help] dialog box. Respond to the error according to
the message shown in the [Action] field.

[Help] dialog box

[Error Message Displays the current errors. If multiple errors exist, errors that have higher priority are
List] displayed at the top.
[Action] Displays the troubleshooting actions for the selected error. Depending on the type of
error, this field may be blank.
[Reset Alarm] Touch to stop the alarm.
[Instruction manual] Touch to display the section of the manual that explains the selected error. This
button cannot be selected if there are no relevant sections.
[Execute]/[Accept] Depending on the error type, either the [Execute] button or the [Accept] button
appears.
Touch [Execute] to execute the action indicated in the [Action] field.
Touch [Accept] to clear the error. Execute the action indicated in the [Action] field.
[Close] Touch to close the [Help] dialog box.

Note:
• After you have closed the [Help] dialog box by touching [Close], you can re-display the dialog box by
touching [Error] in the control menu.
• Even if the [Help] dialog box does not open, you can stop the alarm by touching any part of the screen.

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1.3 Causes of errors and remedial actions

When an error occurs, the [Help] dialog box appears. Refer to the causes and actions described below and take
appropriate action.
If there is more than 1 possible cause, take action in order from case 1.

Risk of infection
When taking remedial action to handle an error, wear adequate personal protective equipment, such as
protective gloves, a protective mask, protective eyewear, and a lab coat. Wash your hands after completing
the task.
There is a risk of infection.

Errors related to the pressure

Abnormal pressure loss

Probable cause Actions

The air pump has malfunctioned. Touch [Execute] in the [Help] dialog box.
An air pump test is executed.

0.06 MPa pressure error

Probable cause Actions

The 0.06 MPa relief valve has malfunctioned. Touch [Execute] in the [Help] dialog box.
An air pump test is executed.

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-0.03 MPa pressure error

Probable cause Actions

Case 1: Touch [Execute] in the [Help] dialog box.

The -0.03 MPa relief valve has malfunctioned. An air pump test is executed.

Case 2: Drain the pneumatic trap chamber. For details, see the
The pneumatic trap chamber is full of water. following.

Draining the pneumatic trap chamber

3 Open the right cover.
Release the lock by turning to the left with a flathead
screwdriver, and open the right cover.
Caution!
If water collects every day, it is possible that a failure
has occurred in the analyzer.
Contact your Sysmex service representative.

1 Shut down the instrument.

1 Make sure the instrument is not in
operation.
2 Remove the sample tube from the
sample tube holder.
(XN-550/XN-450 only)
3 Touch the [Menu] button on the toolbar.
The [Menu] screen appears.

4 Touch the [Shutdown] icon. Right cover

The [Shutdown] dialog box appears.

5 Touch [OK]. Example: XN-550

The instrument power automatically turns OFF.

2 Turn OFF the main power switch.

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4 Turn the pneumatic trap chamber in the 6 Remove the float, and insert into the
direction of the arrow to remove. pneumatic trap chamber.
Insert the float into the pneumatic trap chamber in the
same orientation as when you removed it.

Float
Pneumatic
trap
chamber
Loosen

7 Turn the pneumatic trap chamber in the

direction opposite to that of step 4 to
attach.

8 Close the right cover and lock.

9 Turn ON the main power switch.

Example: XN-550
10 Press the power switch.

1
5 Dispose of the water in the chamber.

Pressure Sensor Error

Probable cause Actions

The pressure sensor has malfunctioned. Remove the sampler adapter (XN-550 only) and sample tubes
from the instrument, and then touch [Execute] in the [Help]
dialog box.
Instrument shutdown is executed. When shutdown is finished,
turn OFF the main power switch. The instrument requires
servicing. Contact your Sysmex service representative.

Vacuum Sensor Error

Probable cause Actions

The vacuum sensor has malfunctioned. Remove the sampler adapter (XN-550 only) and sample tubes
from the instrument, and then touch [Execute] in the [Help]
dialog box.
Instrument shutdown is executed. When shutdown is finished,
turn OFF the main power switch. The instrument requires
servicing. Contact your Sysmex service representative.

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Errors related to the temperature

FCM reaction chamber temperature is high, FCM reaction chamber temperature is low,
Reagent heater temperature is high, Reagent heater temperature is low

Probable cause Actions

The temperature of the unit has fallen out of the monitored Touch [Execute] in the [Help] dialog box.
range. While checking the [Sensor Display] dialog box that appears,
wait for the temperature to return within the monitored range.
(➤P.83 "Chapter 2: 2.11 Checking the instrument status
(sensor)")
If the error has not cleared after 30 minutes, contact your
Sysmex service representative.

Environment temperature is high, Environment temperature is low

Probable cause Actions

The ambient temperature of the instrument has fallen out of Touch [Execute] in the [Help] dialog box.
the usable range (7 to 40°C). Check [Environment Temp.] in the [Sensor Display] dialog box
that appears and adjust the room temperature.
(➤P.83 "Chapter 2: 2.11 Checking the instrument status
(sensor)")

FCM reaction chamber thermistor error, Reagent heater thermistor error,

Environment temperature thermistor error, FCM sheath thermistor error

Probable cause Actions

The thermistor in the unit has malfunctioned, or there is a Remove the sampler adapter (XN-550 only) and sample tubes
circuit break. from the instrument, and then touch [Execute] in the [Help]
dialog box.
Instrument shutdown is executed. When shutdown is finished,
turn OFF the main power switch. The instrument requires
servicing. Contact your Sysmex service representative.

Temperature stabilizing error

Probable cause Actions

The temperature of the unit is not stabilizing. Remove the sampler adapter (XN-550 only) and sample tubes
from the instrument, and then touch [Execute] in the [Help]
dialog box.
Instrument shutdown is executed. When shutdown is finished,
turn OFF the main power switch. The instrument requires
servicing. Contact your Sysmex service representative.

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Errors related to the reagents and chambers

Out of CELLPACK DCL, Out of SULFOLYSER, Out of Lysercell WDF, Out of CELLPACK DFL

Probable cause Actions

Case 1: Remove the object that is pressing on the tubing, and

There is a foreign object pressing on the tubing connected to straighten the tubing.
the reagent container, or there is a kink in the tubing.

Case 2: Touch [Execute] in the [Help] dialog box.

The reagent has run out. The [Reagent Replacement] dialog box appears. Replace the
reagent. For details, see the following.

Replacing the diluent and hemolytic

agent
Note:
In case the reagent outer box label shows a "XN
1 Prepare a new reagent. Reagent Code" or "Reagent Code 2" barcode,
Make sure the reagent has not expired. please scan this barcode.

When the reagent code is entered,

Caution! the reagent in the dialog box shows
• Place the reagent container at a level no more than [Received]. The lot number and
1 meter above or below the bottom of the analyzer.
expiration date show the
Do not place reagents on top of the instrument.
information of the new reagent.
• The new reagent must be allowed to sit for at least
24 hours at room temperature (15 to 35°C) before
use.
• If reagent spills, immediately wipe up the spill
using a moistened cloth.
Note:
When inputting manually, touch the name of the
2 Touch [Execute] in the [Help] dialog reagent to be replaced in the [Reagent Replacement]
box. dialog box. The dialog box below appears.
The [Reagent Replacement] dialog box appears.
(➤P.84 "Chapter 2: 2.12.1 [Reagent Replacement]
dialog box")

3 Input the reagent code (barcode) of the

new reagent.
Input by reading the barcode
Scan the reagent code (barcode) on the outer box of
the new reagent with the hand-held barcode reader.
The reagent code is as shown below.
Make sure the barcode scanning surface is flat before
scanning.
Touch [Replace the reagent.] to select the checkbox,
enter the [Reagent Code :], and touch [OK].

4 Remove the cap from the new reagent

container.

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5 Remove the cap from the old reagent

container. Caution!
Do not touch the aspiration nozzle on the spout set.
Take care that dust does not get on the spout set.

7 Insert the spout set straight into the new

reagent container and close the cap.

Example: Lysercell WDF (2 L)

8 Touch [Execute].
Reagent replacement starts. Wait until this process
finishes. When replacement finishes, the [Reagent
6 Pull the spout set straight up and out. Replacement] dialog box closes.

Time guidelines for reagent replacement are shown

below.

Reagent name Time

CELLPACK DCL Approx. 1 minute
CELLPACK DFL Approx. 3 1/2 minutes
SULFOLYSER Approx. 2 1/2 minutes
Lysercell WDF Approx. 3 1/2 minutes

Out of Fluorocell WDF, Out of Fluorocell RET

Probable cause Actions

The reagent has run out. Replace the reagent. For details, see the following.

Replacing the dye 2 Touch the [Exchange] icon.

The [Exchange] dialog box appears.

3 Touch [Reagent Replacement].

Caution! The [Reagent Replacement] dialog box appears.
While performing replacement, always wear
adequate personal protective equipment.
2 Prepare a new reagent cartridge.
Make sure the reagent has not expired.
1 Touch [Execute] in the [Help] dialog
box.
The [Reagent Replacement] dialog box appears.
(➤P.84 "Chapter 2: 2.12.1 [Reagent Replacement]
dialog box")

When touching [Close] in the [Help] dialog box:

Follow the steps below to display the [Reagent Example: Fluorocell WDF
Replacement] dialog box.

1 Touch the [Maintenance] icon in the

[Menu] screen.
The [Maintenance] menu screen appears.

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3 Pull out the dye holder. 5 Slowly remove the dye cover, taking
care that the dye does not drip.
Dye holder

Dye cover

Caution!
Dye may drip from the nozzle on the dye cover.
If dye drips onto the instrument, immediately wipe it off
using a moistened cloth. Otherwise, the coated surface
of the instrument may become discolored.

6 Remove the old reagent cartridge from

the holder.

Example: XN-550

4 Remove the entire dye holder.

Old reagent cartridge
Fluorocell WDF

7 Insert the new reagent cartridge in the

holder.

Fluorocell RET

Holder
New reagent cartridge

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8 Place the dye cover. 9 Place the holder in the dye holder.

Dye cover 10 Close the dye holder.

The information on the RFID tag of the cartridge is
read, and if normal, a beep sounds. The lot number
and expiration date show the information of the new
reagent.

Information
Be sure to push the dye holder all the way in.

FCM sheath aspiration error, RBC sheath fluid aspiration error

Probable cause Actions

• The tubing connected to the reagent container is clogged. Check the condition of the tubes, and then touch [Execute] in
• There is a foreign object pressing on the tubing connected the [Help] dialog box.
to the reagent container, or there is a kink in the tubing. Reagent replacement takes place.

Fluorocell WDF aspiration error, Fluorocell RET aspiration error

Probable cause Actions

• The dye cover opened. Check the condition of the dye cover, and then touch
• Air bubbles have formed in the tubing connected to the [Execute] in the [Help] dialog box.
cartridge. Reagent replacement takes place.

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Waste chamber 1 not draining, Waste chamber 2 not draining

Probable cause Actions

The drain tubing is clogged. Touch [Execute] in the [Help] dialog box.
The waste chamber is drained.
If the error does not clear, rinse the waste chamber. For
details, see the following.
XN-550
(➤P.20 "Rinsing the waste chamber (XN-550)")
XN-450
(➤P.21 "Rinsing the waste chamber (XN-450)")
XN-350
(➤P.22 "Rinsing the waste chamber (XN-350)")

Rinsing the waste chamber (XN-550)

5 Place CELLCLEAN AUTO in the sample
tube holder.
1 Touch the [Maintenance] icon in the
[Menu] screen. Place in the regular sample tube holder.
The [Maintenance] menu screen appears.

CELLCLEAN
2 Touch the [Rinse Instrument] icon. AUTO

The [Rinse Instrument] dialog box appears.

3 Touch [Rinse Waste Fluid Chamber]. Regular sample

tube holder
The [Rinse Waste Fluid Chamber] dialog box appears.

[Rinse Waste Fluid Chamber] dialog box

6 Press the start switch.
4 Open the sampler cover (manual unit).

The sample tube holder retracts into the instrument

and aspiration starts. When aspiration finishes,
cleaning starts and the sample tube holder is ejected.

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7 Remove CELLCLEAN AUTO. 5 Place CELLCLEAN AUTO in the sample

tube holder.
8 Close the sampler cover (manual unit). Place in the regular sample tube holder.

9 Wait until cleaning finishes.

When the process finishes, the dialog box closes.

Rinsing the waste chamber (XN-450) CELLCLEAN

AUTO
1 Touch the [Maintenance] icon in the Regular
[Menu] screen. sample tube
The [Maintenance] menu screen appears. holder

2 Touch the [Rinse Instrument] icon.

The [Rinse Instrument] dialog box appears.
6 Press the start switch.
3 Touch [Rinse Waste Fluid Chamber].
The [Rinse Waste Fluid Chamber] dialog box appears.

The sample tube holder retracts into the instrument

and aspiration starts. When aspiration finishes,
cleaning starts and the sample tube holder is ejected.

[Rinse Waste Fluid Chamber] dialog box 7 Remove CELLCLEAN AUTO.

4 If the sample tube holder is not ejected, 8 Wait until cleaning finishes.
press the sample tube holder open/close When the process finishes, the dialog box closes.
switch.
The sample tube holder is ejected.

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Rinsing the waste chamber (XN-350)

1 Touch the [Maintenance] icon in the Caution!

• Always wear adequate personal protective
[Menu] screen.
equipment when opening CELLCLEAN AUTO.
The [Maintenance] menu screen appears. • Press down slowly so that the fluid does not spill.
• Store opened CELLCLEAN AUTO standing on the
rack with the opener attached. If CELLCLEAN
2 Touch the [Rinse Instrument] icon. AUTO is tilted, the fluid may leak even with the
The [Rinse Instrument] dialog box appears. opener attached.

3 Touch [Rinse Waste Fluid Chamber]. 5 Insert the pipette all the way to the
The [Rinse Waste Fluid Chamber] dialog box appears. bottom of the CELLCLEAN AUTO, and
press the start switch.

[Rinse Waste Fluid Chamber] dialog box

CELLCLEAN AUTO Start switch

4 Open CELLCLEAN AUTO with the
special CELLCLEAN AUTO opener.
Aspiration starts.
With CELLCLEAN AUTO held straight as shown,
During aspiration, the analysis status indicator LED
press the opener down until you hear a “pop” sound.
blinks green and the beeper sounds repeatedly.
When aspiration finishes, the analysis status indicator
LED turns off and the beeping stops. Cleaning starts.
Opener
6 Remove CELLCLEAN AUTO from the
pipette.
Remove CELLCLEAN AUTO without bending the
pipette.

7 Wait until cleaning finishes.

Keep the opener attached, and remove immediately
before you use CELLCLEAN AUTO. When the process finishes, the dialog box closes.

RBC/HGB chamber not draining

Probable cause Actions

The drain tubing is clogged. Touch [Execute] in the [Help] dialog box.
The sample is drained from the reaction chamber.

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WBCchamb not draining

Probable cause Actions

The drain tubing is clogged. Touch [Execute] in the [Help] dialog box.
The sample is drained from the reaction chamber.

RETchamb not draining

Probable cause Actions

The drain tubing is clogged. Touch [Execute] in the [Help] dialog box.
The sample is drained from the reaction chamber.

Rinse cup pinch valve move error, Waste chamber1 pinch valve move error,
Waste chamber2 pinch valve move error

Probable cause Actions

The pinch valve has malfunctioned. Touch [Execute] in the [Help] dialog box.
A pinch valve test is executed.
If the error does not clear, the device requires servicing.
Contact your Sysmex service representative.

Waste container is full

Probable cause Actions

The waste container is full. Replace the waste container, and then touch [Accept] in the
[Help] dialog box. To replace the waste container, see the
following.

Replacing the waste container

3 Lift the cap with the tube connected
straight up.
Risk of infection Dispose of the full waste container in accordance with
local laws and regulations.
Take care that waste fluid does not splash.

1 Prepare an empty waste container and

remove the cap.

2 Turn the cap on the full waste container

in the direction of the arrow to loosen.

Waste container 4 Insert the cap with the tube connected

straight into the new waste container.

5 Close the cap by turning it in the

direction opposite to that of step 2.

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6 Touch [Accept] in the [Help] dialog box.

Caution!
Install the waste container below the bottom of the
instrument.

Water leak detected

Probable cause Actions

There is a water leak inside the instrument. Touch [Execute] in the [Help] dialog box.
Instrument shutdown is executed. When shutdown is finished,
turn OFF the main power switch, and wipe off the water when
the instrument is wet. The instrument requires servicing.
Contact your Sysmex service representative.

Water leak detected (analysis not possible)

Probable cause Actions

There is a water leak inside the instrument. Touch [Execute] in the [Help] dialog box.
Instrument shutdown is executed. When shutdown is finished,
turn OFF the main power switch. The instrument requires
servicing. Contact your Sysmex service representative.

Water leak sensor error

Probable cause Actions

The water leak sensor has malfunctioned. Remove the sampler adapter (XN-550 only) and sample tubes
from the instrument, and then touch [Execute] in the [Help]
dialog box.
Instrument shutdown is executed. When shutdown is finished,
turn OFF the main power switch. The instrument requires
servicing. Contact your Sysmex service representative.

Out of diluted CELLPACK DST

Probable cause Actions

CELLPACK DST has run out. Check the RU-20. If being prepared, wait briefly.
If the error does not clear, the replacement of CELLPACK DST is
required. For details, see "RU-20 Instructions for Use".
(➤RU-20 Instructions for Use, "Chapter 6: 6.3 Replacing the
reagent")

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Errors related to the motors

WB aspiration motor error

Probable cause Actions

The whole blood aspiration motor has malfunctioned. Touch [Execute] in the [Help] dialog box.
A whole blood aspiration motor test is executed.
If the error does not clear, the instrument requires servicing.
Contact your Sysmex service representative.

Sheath motor error

Probable cause Actions

The sheath motor has malfunctioned. Touch [Execute] in the [Help] dialog box.
A sheath motor test is executed.
If the error does not clear, the instrument requires servicing.
Contact your Sysmex service representative.

Aspiration unit up-down motor error

Probable cause Actions

The aspiration unit up-down motor has malfunctioned. Touch [Execute] in the [Help] dialog box.
Instrument shutdown is executed. When shutdown is finished,
turn the main power switch OFF and then ON.
If the error does not clear, the instrument requires servicing.
Contact your Sysmex service representative.

Aspiration unit front-back motor error

Probable cause Actions

The aspiration unit front-back motor has malfunctioned. Touch [Execute] in the [Help] dialog box.
An aspiration unit motor test is executed.
If the error does not clear, the instrument requires servicing.
Contact your Sysmex service representative.

Tube holder motor move error

Probable cause Actions

The sample tube holder motor has malfunctioned. Remove the sample tubes, and then touch [Execute] in the
[Help] dialog box.
A sample tube holder motor test is executed.
If the error does not clear, the instrument requires servicing.
Contact your Sysmex service representative.

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Errors related to the blood aspiration

Insufficient blood volume (short sample)

Probable cause Actions

Case 1: Touch [Accept] in the [Help] dialog box.

Insufficient blood volume. Prepare sufficient amount of blood.

Case 2: Touch [Accept] in the [Help] dialog box.

The blood is very thin. (The aspiration sensor cannot detect Stop use of the aspiration sensor temporarily in the settings
the blood.) and repeat analysis. For details on the aspiration sensor
settings, see "Basic Operation". (➤Basic Operation, "Chapter
7: 7.9.1 Aspiration sensor settings")

Case 3: Touch [Accept] in the [Help] dialog box.

The piercer (XN-550/XN-450), pipette (XN-350), or whole Execute auto rinse. For details, see the following.
blood aspiration line tubing is clogged. (➤P.27 "Executing auto rinse")

If the error persists

Execute routine cleaning. For details, see the following.
XN-550
(➤P.27 "Executing routine cleaning (XN-550)")
XN-450
(➤P.28 "Executing routine cleaning (XN-450)")
XN-350
(➤P.29 "Executing routine cleaning (XN-350)")

If the error persists

Replace the piercer (XN-550/XN-450). Contact your Sysmex
service representative.

Blood cannot be aspirated.

Probable cause Actions

Case 1: Touch [Accept] in the [Help] dialog box.

The density of the sample is inconsistent. Mix the sample well and then analyze.

Case 2: Execute auto rinse. For details, see the following.

The piercer (XN-550/XN-450), pipette (XN-350), or whole (➤P.27 "Executing auto rinse")
blood aspiration line tubing is clogged.
If the error persists
Execute routine cleaning. For details, see the following.
XN-550
(➤P.27 "Executing routine cleaning (XN-550)")
XN-450
(➤P.28 "Executing routine cleaning (XN-450)")
XN-350
(➤P.29 "Executing routine cleaning (XN-350)")

If the error persists

Replace the piercer (XN-550/XN-450). Contact your Sysmex
service representative.

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Executing auto rinse

3 Touch [Routine Cleaning].
1 Touch the [Auto Rinse] icon in the The [Routine Cleaning] dialog box appears.
[Menu] screen.
1 The following dialog box appears.

[Routine Cleaning] dialog box

2 Touch [Yes].
Auto rinse starts and the [Auto Rinse] dialog box 4 Open the sampler cover (manual unit).
appears.
5 Place CELLCLEAN AUTO in the sample
tube holder.
Place in the regular sample tube holder.

CELLCLEAN
AUTO

[Auto Rinse] dialog box

Wait until this process finishes. When the process Regular sample
finishes, the dialog box closes and a background tube holder
check starts.

Executing routine cleaning (XN-550)

1 Touch the [Maintenance] icon in the

[Menu] screen.
The [Maintenance] menu screen appears.

2 Touch the [Rinse Instrument] icon.

The [Rinse Instrument] dialog box appears.

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6 Press the start switch. 4 If the sample tube holder is not ejected,
press the sample tube holder open/close
switch.
The sample tube holder is ejected.

The sample tube holder retracts into the instrument

and aspiration starts. When aspiration finishes,
cleaning starts and the sample tube holder is ejected.

5 Place CELLCLEAN AUTO in the sample

7 Remove CELLCLEAN AUTO. tube holder.
Place in the regular sample tube holder.
8 Close the sampler cover (manual unit).

9 Wait until cleaning finishes.

When the process finishes, the dialog box closes, and
the instrument power automatically turns OFF.
CELLCLEAN
AUTO
Executing routine cleaning (XN-450)
Regular
1 Touch the [Maintenance] icon in the sample tube
holder
[Menu] screen.
The [Maintenance] menu screen appears.

2 Touch the [Rinse Instrument] icon. 6 Press the start switch.

The [Rinse Instrument] dialog box appears.

3 Touch [Routine Cleaning].

The [Routine Cleaning] dialog box appears.

The sample tube holder retracts into the instrument

and aspiration starts. When aspiration finishes,
cleaning starts and the sample tube holder is ejected.

7 Remove CELLCLEAN AUTO.

[Routine Cleaning] dialog box
8 Wait until cleaning finishes.
When the process finishes, the dialog box closes, and
the instrument power automatically turns OFF.

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Executing routine cleaning (XN-350)

1 Touch the [Maintenance] icon in the Caution!
[Menu] screen. • Always wear adequate personal protective
The [Maintenance] menu screen appears. equipment when opening CELLCLEAN AUTO.
• Press down slowly so that the fluid does not spill.
• Store opened CELLCLEAN AUTO standing on the
2 Touch the [Rinse Instrument] icon. rack with the opener attached. If CELLCLEAN
AUTO is tilted, the fluid may leak even with the
The [Rinse Instrument] dialog box appears.
opener attached.

3 Touch [Routine Cleaning].

The [Routine Cleaning] dialog box appears.
5 Insert the pipette all the way to the
bottom of the CELLCLEAN AUTO, and
press the start switch.

[Routine Cleaning] dialog box

4 Open CELLCLEAN AUTO with the CELLCLEAN AUTO Start switch

special CELLCLEAN AUTO opener.
With CELLCLEAN AUTO held straight as shown, Aspiration starts.
press the opener down until you hear a “pop” sound. During aspiration, the analysis status indicator LED
blinks green and the beeper sounds repeatedly.
When aspiration finishes, the analysis status indicator
Opener LED turns off and the beeping stops. Cleaning starts.

6 Remove CELLCLEAN AUTO from the

pipette.
Remove CELLCLEAN AUTO without bending the
pipette.

Keep the opener attached, and remove immediately 7 Wait until cleaning finishes.
before you use CELLCLEAN AUTO.
When the process finishes, the dialog box closes, and
the instrument power automatically turns OFF.

Aspiration Sensor error

Probable cause Actions

The aspiration sensor has malfunctioned. Touch [Accept] in the [Help] dialog box.
The instrument requires servicing. Contact your Sysmex
service representative.

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Errors related to the sample tube holder

Tube remains in tube holder

Probable cause Actions

A sample tube was still in the sample tube holder when Remove the sample tube, and then touch [Accept] in the
analysis started. The hand did not work properly during [Help] dialog box.
sampler analysis.

Two tubes are in tube holder

Probable cause Actions

Both a regular sample tube and a micro collection tube were Remove the sample tube that will not be analyzed, and then
set for manual analysis. touch [Accept] in the [Help] dialog box.

Failed to acquire items.

Probable cause Actions

The set amount of time (60 seconds) elapsed after the host Pull out the sampler adapter holder and remix the sample
computer was queried for analysis information during sampler tube in the starting tube position (adapter number – test
analysis. tube position) shown in the instrument status on the left side
of the control menu. Replace the sample tube in its original
position, push in the sampler adapter holder, and touch
[Accept] in the [Help] dialog box.

Errors related to the sample numbers

Sample number not input

Probable cause Actions

No sample number was specified at the time of manual Touch [Accept] in the [Help] dialog box.
analysis. Enter the sample number and then execute the analysis.

Failed to read sample number.

Probable cause Actions

The barcode label is dirty. Touch [Accept] in the [Help] dialog box. Check the position
The print condition of the barcode label is poor. and orientation of the barcode label, and if the barcode label is
The position or orientation of the barcode label is poor. dirty.

Errors related to the orders

Analysis item not specified

Probable cause Actions

No analysis item was specified at the time of manual analysis. Touch [Accept] in the [Help] dialog box.
Specify the analysis item and then execute analysis.

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Invalid analysis item is specified

Probable cause Actions

An invalid analysis item is specified. Touch [Accept] in the [Help] dialog box.
Change the analysis item and then execute analysis.

Invalid analysis item is specified (sampler analysis)

Probable cause Actions

During sampler analysis, a sample had an analysis parameter Touch [Accept] in the [Help] dialog box. The error sample is
that cannot be analyzed. skipped and sampler analysis continues. When sampler
analysis finishes, change the analysis parameter of the
skipped sample and then analyze the sample.

Errors related to the laser

Laser life

Probable cause Actions

It is time to replace the laser. Laser replacement is required. Contact your Sysmex service
representative.

Laser output error

Probable cause Actions

• The FCM cover opened. Remove the sampler adapter (XN-550 only) and sample tubes
• The FCM cover sensor has malfunctioned. from the instrument, and then touch [Execute] in the [Help]
dialog box.
Instrument shutdown is executed. When shutdown is finished,
turn OFF the main power switch. The instrument requires
servicing. Contact your Sysmex service representative.

Errors related to the system

Barcode reader communication error

Probable cause Actions

There was a communication error with the barcode reader. Remove the sampler adapter (XN-550 only) and sample tubes
from the instrument, and then touch [Execute] in the [Help]
dialog box.
Instrument shutdown is executed. When shutdown is finished,
turn the main power switch OFF and then ON.
If the error persists, contact your Sysmex service
representative.

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RFID communication error

Probable cause Actions

There was a communication error with the RFID reader/writer. Remove the sampler adapter (XN-550 only) and sample tubes
from the instrument, and then touch [Execute] in the [Help]
dialog box.
Instrument shutdown is executed. When shutdown is finished,
turn the main power switch OFF and then ON.
If the error persists, contact your Sysmex service
representative.

Internal Error

Probable cause Actions

An error occurred in the operation of the program. Remove the sampler adapter (XN-550 only) and sample tubes
from the instrument, and then touch [Execute] in the [Help]
dialog box.
Instrument shutdown is executed. When shutdown is finished,
turn OFF the main power switch. The instrument requires
servicing. Contact your Sysmex service representative.

Errors related to the quality control (QC)

X-barM control error, L-J Control Error, X-bar control error

Probable cause Actions

A QC error (value out of the control limit range) occurred. Touch [Accept] in the [Help] dialog box.
Check the analysis results in the [QC Chart] screen, and
perform calibration if necessary. For details on checking the
QC results, see "Basic Operation".
(➤Basic Operation, "Chapter 3: 3.7 Checking analysis results")

Control has expired.

Probable cause Actions

The control blood has expired. Touch [Accept] in the [Help] dialog box.
Use new control blood to execute QC analysis.
When using a new lot, the lot information must be registered in
a new QC file. For details on registering lot information, see
"Basic Operation".
(➤Basic Operation, "Chapter 3: 3.5 Registering lots")

Control is not entered.

Probable cause Actions

Control blood with an unregistered lot number was used. Touch [Accept] in the [Help] dialog box.
Register the control blood lot information. For details on
registering lot information, see "Basic Operation".
(➤Basic Operation, "Chapter 3: 3.5 Registering lots")

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QC not executed.

Probable cause Actions

It is time to execute QC analysis. Touch [Accept] in the [Help] dialog box.

Execute QC. For details on QC, see "Basic Operation".
(➤Basic Operation, "Chapter 3: Performing Quality Control (QC)")

WDF Scattergram sensitivity error, RET Scattergram sensitivity error

Probable cause Actions

A numerical value for a parameter in the scattergram is Touch [Accept] in the [Help] dialog box.
outside the specified range. Check the scattergram in the [Data Browser] screen. For
details on the [Data Browser] screen, see "Basic Operation".
(➤Basic Operation, "Chapter 6: Checking Detailed Analysis
Information (Data Browser)")

Check Measurement Mode

Probable cause Actions

The analysis mode is not compatible with the control blood Touch [Accept] in the [Help] dialog box.
type. Change the analysis mode and then analyze.

Errors related to the analysis

Background check error

Probable cause Actions

Air bubbles have formed in the detector. Touch [Accept] in the [Help] dialog box.
The detector is clogged. Execute auto rinse. For details, see the following.
The detector is dirty.

Executing auto rinse

2 Touch [Yes].
1 Touch the [Auto Rinse] icon in the Auto rinse starts and the [Auto Rinse] dialog box
[Menu] screen. appears.
1 The following dialog box appears.

[Auto Rinse] dialog box

Wait until this process finishes. When the process

finishes, the dialog box closes and a background
check starts.

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RBC sampling error, PLT sampling error

Probable cause Actions

Case 1: Touch [Accept] in the [Help] dialog box.

The density of the sample is inconsistent. Mix the sample well and then re-analyze.

Case 2: [PLT sampling error]

The detector suddenly became clogged. Drain the sample from the RBC isolation chamber. For details,
see the following.
(➤P.34 "Draining the sample from the RBC isolation
chamber")

If the error persists

Remove the clog from the RBC detector. For details, see the
following.
(➤P.34 "Removing the RBC detector clog")

If the error persists

Rinse the RBC detector aperture. For details, see the
following.
(➤P.35 "Rinsing the RBC detector aperture")

Draining the sample from the RBC Removing the RBC detector clog
isolation chamber
1 Touch the [Maintenance] icon in the
1 Touch the [Maintenance] icon in the [Menu] screen.
[Menu] screen. The [Maintenance] menu screen appears.
The [Maintenance] menu screen appears.
2 Touch the [Rinse Instrument] icon.
2 Touch the [Rinse Instrument] icon. The [Rinse Instrument] dialog box appears.
The [Rinse Instrument] dialog box appears.
3 Touch [Remove RBC Detector Clog].
3 Touch [Drain RBC Isolation Chamber]. Clog removal starts, and the [Remove RBC Detector
Clog] dialog box appears.
Draining starts, and the [Drain RBC Isolation
Chamber] dialog box appears.

[Remove RBC Detector Clog] dialog box

[Drain RBC Isolation Chamber] dialog box Wait until this process finishes. When the process
Wait until this process finishes. When the process finishes, the dialog box closes.
finishes, the dialog box closes.

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Rinsing the RBC detector aperture

3 Turn OFF the main power switch.

Warning!
Never touch the detector when the power of the
instrument is turned ON.
An electrical shock could occur.

Caution!
• Be sure to use CELLCLEAN AUTO only.
• When closing the detector cover, take care not to
kink the tube. It may lead to incorrect analysis.
• When rinsing the detector aperture, use the
supplied unclogging brush and lightly tap on the
detector aperture.
Excessive force will damage the detector aperture.

1 Open CELLCLEAN AUTO with the 4 Open the right cover.

special CELLCLEAN AUTO opener. Release the lock by turning to the left with a flathead
With CELLCLEAN AUTO held straight as shown, screwdriver, and open the right cover.
press the opener down until you hear a “pop” sound.

Opener

Keep the opener attached, and remove immediately

before you use CELLCLEAN AUTO.

Caution!
• Always wear adequate personal protective Right cover
equipment when opening CELLCLEAN AUTO.
• Press down slowly so that the fluid does not spill.
• Store opened CELLCLEAN AUTO standing on the Example: XN-550
rack with the opener attached. If CELLCLEAN
AUTO is tilted, the fluid may leak even with the
opener attached.

2 Shut down the instrument.

1 Touch the [Menu] button on the toolbar.
The [Menu] screen appears.

2 Touch the [Shutdown] icon.

The [Shutdown] dialog box appears.

3 Touch [OK].
The instrument power automatically turns OFF.

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5 Loosen the screw that fastens the 7 Turn the lid of the detector chamber in
detector cover. the direction of the arrow and pull out.

Lid of the detector

chamber

Detector
cover

8 Soak the supplied unclogging brush in

the CELLCLEAN AUTO fluid, and wash
the detector aperture by lightly tapping it.

Detector aperture
Example: XN-550

6 Remove the detector cover.

Lift and pull out toward you.

Detector cover

Unclogging brush

Note:
If fluid spills, wipe off the spilled fluid with a piece of
tissue paper.

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9 Insert the lid of the detector chamber 10 Install the detector cover, and fasten the
straight in, and turn in the direction of screw with the flathead screwdriver.
the arrow to lock.
Insert the lid of the detector chamber all the way in, 11 Close the right cover and lock.
and turn to the position shown.

12 Turn ON the main power switch.

13 Press the power switch.

Information
Wash the brush and opener well and store in a clean
state.
There is a risk of instrument malfunctioning if there
are small particles or other contaminants on the brush
or opener.
Caution!
When the lid of the detector chamber is not properly
attached, correct results are not obtained.
The instrument may be damaged if leakage occurs.

WDF sampling error, RET sampling error

Probable cause Actions

Case 1: Touch [Accept] in the [Help] dialog box.

The density of the sample is inconsistent. Mix the sample well and then re-analyze.

Case 2: Rinse the flowcell. For details, see the following.

The flowcell has suddenly become clogged. XN-550
(➤P.37 "Rinsing the flowcell (XN-550)")
XN-450
(➤P.38 "Rinsing the flowcell (XN-450)")
XN-350
(➤P.39 "Rinsing the flowcell (XN-350)")

Rinsing the flowcell (XN-550)

3 Touch [Rinse Flowcell].
1 Touch the [Maintenance] icon in the The [Rinse Flowcell] dialog box appears.
[Menu] screen.
The [Maintenance] menu screen appears.

2 Touch the [Rinse Instrument] icon.

The [Rinse Instrument] dialog box appears.

[Rinse Flowcell] dialog box

4 Open the sampler cover (manual unit).

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Rinsing the flowcell (XN-450)

5 Place CELLCLEAN AUTO in the sample
tube holder. 1 Touch the [Maintenance] icon in the
Place in the regular sample tube holder. [Menu] screen.
The [Maintenance] menu screen appears.

CELLCLEAN
AUTO 2 Touch the [Rinse Instrument] icon.
The [Rinse Instrument] dialog box appears.

Regular sample 3 Touch [Rinse Flowcell].

tube holder
The [Rinse Flowcell] dialog box appears.

[Rinse Flowcell] dialog box

6 Press the start switch.
4 If the sample tube holder is not ejected,
press the sample tube holder open/close
switch.
The sample tube holder is ejected.

The sample tube holder retracts into the instrument

and aspiration starts. When aspiration finishes,
cleaning starts and the sample tube holder is ejected.

7 Remove CELLCLEAN AUTO. 5 Place CELLCLEAN AUTO in the sample

tube holder.
8 Close the sampler cover (manual unit). Place in the regular sample tube holder.

9 Wait until cleaning finishes.

When the process finishes, the dialog box closes.

CELLCLEAN
AUTO

Regular
sample tube
holder

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Rinsing the flowcell (XN-350)

6 Press the start switch.
1 Touch the [Maintenance] icon in the
[Menu] screen.
The [Maintenance] menu screen appears.

2 Touch the [Rinse Instrument] icon.

The [Rinse Instrument] dialog box appears.

3 Touch [Rinse Flowcell].

The [Rinse Flowcell] dialog box appears.

The sample tube holder retracts into the instrument

and aspiration starts. When aspiration finishes,
cleaning starts and the sample tube holder is ejected.

7 Remove CELLCLEAN AUTO.

8 Wait until cleaning finishes.

When the process finishes, the dialog box closes.

[Rinse Flowcell] dialog box

4 Open CELLCLEAN AUTO with the

special CELLCLEAN AUTO opener.
With CELLCLEAN AUTO held straight as shown,
press the opener down until you hear a “pop” sound.

Opener

Keep the opener attached, and remove immediately

before you use CELLCLEAN AUTO.

Caution!
• Always wear adequate personal protective
equipment when opening CELLCLEAN AUTO.
• Press down slowly so that the fluid does not spill.
• Store opened CELLCLEAN AUTO standing on the
rack with the opener attached. If CELLCLEAN
AUTO is tilted, the fluid may leak even with the
opener attached.

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5 Insert the pipette all the way to the 6 Remove CELLCLEAN AUTO from the
bottom of the CELLCLEAN AUTO, and pipette.
press the start switch. Remove CELLCLEAN AUTO without bending the
pipette.

7 Wait until cleaning finishes.

When the process finishes, the dialog box closes.

CELLCLEAN AUTO Start switch

Aspiration starts.
During aspiration, the analysis status indicator LED
blinks green and the beeper sounds repeatedly.
When aspiration finishes, the analysis status indicator
LED turns off and the beeping stops. Cleaning starts.

RBC detector clog, Bubbles in RBC detector

Probable cause Actions

The detector is clogged. Touch [Execute] in the [Help] dialog box.

Air bubbles have formed in the detector. Remove the clog from the RBC detector.
If the error persists, rinse the RBC detector aperture. For
details, see the following.

Rinsing the RBC detector aperture

1 Open CELLCLEAN AUTO with the Caution!
special CELLCLEAN AUTO opener. • Always wear adequate personal protective
With CELLCLEAN AUTO held straight as shown, equipment when opening CELLCLEAN AUTO.
• Press down slowly so that the fluid does not spill.
press the opener down until you hear a “pop” sound.
• Store opened CELLCLEAN AUTO standing on the
rack with the opener attached. If CELLCLEAN
AUTO is tilted, the fluid may leak even with the
Opener opener attached.

2 Shut down the instrument.

1 Touch the [Menu] button on the toolbar.
The [Menu] screen appears.

Keep the opener attached, and remove immediately

2 Touch the [Shutdown] icon.
before you use CELLCLEAN AUTO. The [Shutdown] dialog box appears.

3 Touch [OK].
The instrument power automatically turns OFF.

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3 Turn OFF the main power switch. 5 Loosen the screw that fastens the
detector cover.

Detector
cover

4 Open the right cover.

Release the lock by turning to the left with a flathead
screwdriver, and open the right cover.

Example: XN-550

6 Remove the detector cover.

Lift and pull out toward you.

Detector cover

Right cover

Example: XN-550

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7 Turn the lid of the detector chamber in 9 Insert the lid of the detector chamber
the direction of the arrow and pull out. straight in, and turn in the direction of
the arrow to lock.
Insert the lid of the detector chamber all the way in,
and turn to the position shown.
Lid of the detector
chamber

Caution!
When the lid of the detector chamber is not properly
attached, correct results are not obtained.
The instrument may be damaged if leakage occurs.

8 Soak the supplied unclogging brush in

the CELLCLEAN AUTO fluid, and wash 10 Install the detector cover, and fasten the
the detector aperture by lightly tapping it. screw with the flathead screwdriver.

Detector aperture
11 Close the right cover and lock.

12 Turn ON the main power switch.

13 Press the power switch.

Information
Wash the brush and opener well and store in a clean
state.
Unclogging brush There is a risk of instrument malfunctioning if there
are small particles or other contaminants on the brush
or opener.

Note:
If fluid spills, wipe off the spilled fluid with a piece of
tissue paper.

HGB error

Probable cause Actions

The HGB blank value or the HGB sample value has fallen out Touch [Accept] in the [Help] dialog box.
of the specified range. Re-analyze.

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RBC channel error, PLT channel error, WDF channel error

Probable cause Actions

Case 1: Touch [Accept] in the [Help] dialog box.

The density of the sample is inconsistent. Mix the sample well and then re-analyze.

Case 2: Make sure there are no sources of noise near the instrument.
Because of external noise, the particle count has exceeded Move any noise sources away from the instrument and re-
the limit of the display range. analyze.

RET channel error

Probable cause Actions

Case 1: Touch [Accept] in the [Help] dialog box.

The density of the sample is inconsistent. Mix the sample well and then re-analyze.

Case 2: Rinse the flowcell. For details, see the following.

The flowcell is clogged or dirty. XN-550
Air bubbles have formed in the flowcell. (➤P.43 "Rinsing the flowcell (XN-550)")
XN-450
(➤P.44 "Rinsing the flowcell (XN-450)")
XN-350
(➤P.45 "Rinsing the flowcell (XN-350)")

If the error persists

Remove air bubbles from the flowcell. For details, see the
following.
(➤P.46 "Removing air bubbles from the flowcell")

Rinsing the flowcell (XN-550)

3 Touch [Rinse Flowcell].
1 Touch the [Maintenance] icon in the The [Rinse Flowcell] dialog box appears.
[Menu] screen.
The [Maintenance] menu screen appears.

2 Touch the [Rinse Instrument] icon.

The [Rinse Instrument] dialog box appears.

[Rinse Flowcell] dialog box

4 Open the sampler cover (manual unit).

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Rinsing the flowcell (XN-450)

5 Place CELLCLEAN AUTO in the sample
tube holder. 1 Touch the [Maintenance] icon in the
Place in the regular sample tube holder. [Menu] screen.
The [Maintenance] menu screen appears.

CELLCLEAN
AUTO 2 Touch the [Rinse Instrument] icon.
The [Rinse Instrument] dialog box appears.

Regular sample 3 Touch [Rinse Flowcell].

tube holder
The [Rinse Flowcell] dialog box appears.

[Rinse Flowcell] dialog box

6 Press the start switch.
4 If the sample tube holder is not ejected,
press the sample tube holder open/close
switch.
The sample tube holder is ejected.

The sample tube holder retracts into the instrument

and aspiration starts. When aspiration finishes,
cleaning starts and the sample tube holder is ejected.

7 Remove CELLCLEAN AUTO. 5 Place CELLCLEAN AUTO in the sample

tube holder.
8 Close the sampler cover (manual unit). Place in the regular sample tube holder.

9 Wait until cleaning finishes.

When the process finishes, the dialog box closes.

CELLCLEAN
AUTO

Regular
sample tube
holder

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6 Press the start switch. 4 Open CELLCLEAN AUTO with the

special CELLCLEAN AUTO opener.
With CELLCLEAN AUTO held straight as shown,
press the opener down until you hear a “pop” sound.

Opener

The sample tube holder retracts into the instrument

and aspiration starts. When aspiration finishes,
cleaning starts and the sample tube holder is ejected. Keep the opener attached, and remove immediately
before you use CELLCLEAN AUTO.

7 Remove CELLCLEAN AUTO.

Caution!
8 Wait until cleaning finishes. • Always wear adequate personal protective
equipment when opening CELLCLEAN AUTO.
When the process finishes, the dialog box closes.
• Press down slowly so that the fluid does not spill.
• Store opened CELLCLEAN AUTO standing on the
Rinsing the flowcell (XN-350) rack with the opener attached. If CELLCLEAN
AUTO is tilted, the fluid may leak even with the
opener attached.
1 Touch the [Maintenance] icon in the
[Menu] screen.
The [Maintenance] menu screen appears. 5 Insert the pipette all the way to the
bottom of the CELLCLEAN AUTO, and
2 Touch the [Rinse Instrument] icon. press the start switch.
The [Rinse Instrument] dialog box appears.

3 Touch [Rinse Flowcell].

The [Rinse Flowcell] dialog box appears.

CELLCLEAN AUTO Start switch

Aspiration starts.
During aspiration, the analysis status indicator LED
blinks green and the beeper sounds repeatedly.
[Rinse Flowcell] dialog box When aspiration finishes, the analysis status indicator
LED turns off and the beeping stops. Cleaning starts.

6 Remove CELLCLEAN AUTO from the

pipette.
Remove CELLCLEAN AUTO without bending the
pipette.

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7 Wait until cleaning finishes. 3 Touch [Remove Flowcell Air Bubbles].

When the process finishes, the dialog box closes. Bubble removal starts and the [Remove Flowcell Air
Bubbles] dialog box appears.

Removing air bubbles from the flowcell

1 Touch the [Maintenance] icon in the
[Menu] screen.
The [Maintenance] menu screen appears.

[Remove Flowcell Air Bubbles] dialog box

2 Touch the [Rinse Instrument] icon.
The [Rinse Instrument] dialog box appears. Wait until this process finishes. When the process
finishes, the dialog box closes.

Data Errors

Probable cause Actions

An analysis value was outside the set upper/lower limit range. Touch [Accept] in the [Help] dialog box. Check the analysis
data and review the set upper and lower limit values.

Analysis result is high

Probable cause Actions

When body fluid analysis was executed, analysis data with Remove the sample tubes from the instrument, and then
high values that may affect the next analysis results were touch [Execute] in the [Help] dialog box.
obtained. A background check is executed.

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Low count error

Probable cause Actions

The piercer (XN-550/XN-450), pipette (XN-350), or whole Touch [Accept] in the [Help] dialog box. Execute auto rinse.
blood aspiration line tubing is clogged. For details, see the following.
(➤P.47 "Executing auto rinse")

If the error persists:

Execute routine cleaning. For details, see the following.

XN-550
(➤P.47 "Executing routine cleaning (XN-550)")
XN-450
(➤P.48 "Executing routine cleaning (XN-450)")
XN-350
(➤P.49 "Executing routine cleaning (XN-350)")

If the error persists

Replace the piercer (XN-550/XN-450). Contact your Sysmex
service representative.

Executing auto rinse Executing routine cleaning (XN-550)

1 Touch the [Auto Rinse] icon in the 1 Touch the [Maintenance] icon in the
[Menu] screen. [Menu] screen.
1 The following dialog box appears.
The [Maintenance] menu screen appears.

2 Touch the [Rinse Instrument] icon.

The [Rinse Instrument] dialog box appears.

3 Touch [Routine Cleaning].

The [Routine Cleaning] dialog box appears.

2 Touch [Yes].
Auto rinse starts and the [Auto Rinse] dialog box
appears.

[Routine Cleaning] dialog box

[Auto Rinse] dialog box 4 Open the sampler cover (manual unit).
Wait until this process finishes. When the process
finishes, the dialog box closes and a background
check starts.

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5 Place CELLCLEAN AUTO in the sample 2 Touch the [Rinse Instrument] icon.
tube holder. The [Rinse Instrument] dialog box appears.
Place in the regular sample tube holder.
3 Touch [Routine Cleaning].
CELLCLEAN The [Routine Cleaning] dialog box appears.
AUTO

Regular sample
tube holder

[Routine Cleaning] dialog box

4 If the sample tube holder is not ejected,

press the sample tube holder open/close
switch.
The sample tube holder is ejected.
6 Press the start switch.

5 Place CELLCLEAN AUTO in the sample

The sample tube holder retracts into the instrument tube holder.
and aspiration starts. When aspiration finishes, Place in the regular sample tube holder.
cleaning starts and the sample tube holder is ejected.

7 Remove CELLCLEAN AUTO.

8 Close the sampler cover (manual unit).

CELLCLEAN
AUTO
9 Wait until cleaning finishes.
When the process finishes, the dialog box closes, and Regular
the instrument power automatically turns OFF. sample tube
holder

Executing routine cleaning (XN-450)

1 Touch the [Maintenance] icon in the
[Menu] screen.
The [Maintenance] menu screen appears.

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6 Press the start switch. 4 Open CELLCLEAN AUTO with the

special CELLCLEAN AUTO opener.
With CELLCLEAN AUTO held straight as shown,
press the opener down until you hear a “pop” sound.

Opener

The sample tube holder retracts into the instrument

and aspiration starts. When aspiration finishes,
cleaning starts and the sample tube holder is ejected. Keep the opener attached, and remove immediately
before you use CELLCLEAN AUTO.

7 Remove CELLCLEAN AUTO.

Caution!
8 Wait until cleaning finishes. • Always wear adequate personal protective
equipment when opening CELLCLEAN AUTO.
When the process finishes, the dialog box closes, and
• Press down slowly so that the fluid does not spill.
the instrument power automatically turns OFF.
• Store opened CELLCLEAN AUTO standing on the
rack with the opener attached. If CELLCLEAN
AUTO is tilted, the fluid may leak even with the
Executing routine cleaning (XN-350) opener attached.

1 Touch the [Maintenance] icon in the

[Menu] screen. 5 Insert the pipette all the way to the
The [Maintenance] menu screen appears. bottom of the CELLCLEAN AUTO, and
press the start switch.
2 Touch the [Rinse Instrument] icon.
The [Rinse Instrument] dialog box appears.

3 Touch [Routine Cleaning].

The [Routine Cleaning] dialog box appears.

CELLCLEAN AUTO Start switch

Aspiration starts.
During aspiration, the analysis status indicator LED
blinks green and the beeper sounds repeatedly.
[Routine Cleaning] dialog box When aspiration finishes, the analysis status indicator
LED turns off and the beeping stops. Cleaning starts.

6 Remove CELLCLEAN AUTO from the

pipette.
Remove CELLCLEAN AUTO without bending the
pipette.

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7 Wait until cleaning finishes.

When the process finishes, the dialog box closes, and
the instrument power automatically turns OFF.

Errors related to the covers

FCM cover is open.

Probable cause Actions

The FCM detector cover is open. Contact your Sysmex service representative.

The dye holder is open.

Probable cause Actions

The dye holder has been pulled out. Push the dye holder into the instrument.

Right cover is open

Probable cause Actions

The right cover is open. Close the right cover.

Right cover is opened

Probable cause Actions

The right cover opened while the instrument was in operation. Close the right cover and then touch [Execute] in the [Help]
dialog box.
When shutdown is finished, turn OFF the main power switch.
Turn ON the main power switch again and press the power
switch.

The tube holder is opened.

Probable cause Actions

The sample tube holder opened while the instrument was in Touch [Execute] in the [Help] dialog box.
operation. When shutdown is finished, turn OFF the main power switch.
Turn ON the main power switch again and press the power
switch.

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Errors related to the user maintenance and warnings

Piercer replacement is required.

Probable cause Actions

It is time to replace the piercer. Replace the piercer (XN-550/XN-450). Contact your Sysmex
service representative.

Execute Routine Cleaning

Probable cause Actions

It is time to execute routine cleaning. Touch [Accept] in the [Help] dialog box.
(The cumulative analysis count since the previous routine Execute routine cleaning. For details, see the following.
cleaning was performed has exceeded 1,000, or 1 week has XN-550
elapsed.) (➤P.51 "Executing routine cleaning (XN-550)")
XN-450
(➤P.52 "Executing routine cleaning (XN-450)")
XN-350
(➤P.53 "Executing routine cleaning (XN-350)")

Execute Routine Cleaning (warning)

Probable cause Actions

Routine cleaning must be executed. Touch [Execute] in the [Help] dialog box.
(The cumulative analysis count since the previous routine The [Routine Cleaning] dialog box appears. Execute routine
cleaning was performed has exceeded 1,400.) cleaning. For details, see the following.
XN-550
(➤P.51 "Executing routine cleaning (XN-550)")
XN-450
(➤P.52 "Executing routine cleaning (XN-450)")
XN-350
(➤P.53 "Executing routine cleaning (XN-350)")

Executing routine cleaning (XN-550)

When [Execute Routine Cleaning (warning)] appears,
3 Touch [Routine Cleaning].
touch [Execute] in the [Help] dialog box. The [Routine The [Routine Cleaning] dialog box appears.
Cleaning] dialog box appears. Follow the procedure from
step 4.

1 Touch the [Maintenance] icon in the

[Menu] screen.
The [Maintenance] menu screen appears.

2 Touch the [Rinse Instrument] icon.

The [Rinse Instrument] dialog box appears.

[Routine Cleaning] dialog box

4 Open the sampler cover (manual unit).

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Executing routine cleaning (XN-450)

5 Place CELLCLEAN AUTO in the sample When [Execute Routine Cleaning (warning)] appears,
tube holder. touch [Execute] in the [Help] dialog box. The [Routine
Place in the regular sample tube holder. Cleaning] dialog box appears. Follow the procedure from
step 4.

CELLCLEAN
AUTO
1 Touch the [Maintenance] icon in the
[Menu] screen.
The [Maintenance] menu screen appears.

Regular sample
tube holder 2 Touch the [Rinse Instrument] icon.
The [Rinse Instrument] dialog box appears.

3 Touch [Routine Cleaning].

The [Routine Cleaning] dialog box appears.

6 Press the start switch.

[Routine Cleaning] dialog box

4 If the sample tube holder is not ejected,

press the sample tube holder open/close
switch.
The sample tube holder is ejected.

The sample tube holder retracts into the instrument

and aspiration starts. When aspiration finishes,
cleaning starts and the sample tube holder is ejected.

7 Remove CELLCLEAN AUTO.

8 Close the sampler cover (manual unit).

9 Wait until cleaning finishes.

When the process finishes, the dialog box closes, and
the instrument power automatically turns OFF.

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5 Place CELLCLEAN AUTO in the sample 3 Touch [Routine Cleaning].

tube holder. The [Routine Cleaning] dialog box appears.
Place in the regular sample tube holder.

CELLCLEAN
AUTO

Regular
sample tube
holder

[Routine Cleaning] dialog box

6 Press the start switch. 4 Open CELLCLEAN AUTO with the

special CELLCLEAN AUTO opener.
With CELLCLEAN AUTO held straight as shown,
press the opener down until you hear a “pop” sound.

Opener

The sample tube holder retracts into the instrument

and aspiration starts. When aspiration finishes,
Keep the opener attached, and remove immediately
cleaning starts and the sample tube holder is ejected.
before you use CELLCLEAN AUTO.

7 Remove CELLCLEAN AUTO.

Caution!
8 Wait until cleaning finishes. • Always wear adequate personal protective
equipment when opening CELLCLEAN AUTO.
When the process finishes, the dialog box closes, and • Press down slowly so that the fluid does not spill.
the instrument power automatically turns OFF. • Store opened CELLCLEAN AUTO standing on the
rack with the opener attached. If CELLCLEAN
AUTO is tilted, the fluid may leak even with the
Executing routine cleaning (XN-350) opener attached.
When [Execute Routine Cleaning (warning)] appears,
touch [Execute] in the [Help] dialog box. The [Routine
Cleaning] dialog box appears. Follow the procedure from
step 4.

1 Touch the [Maintenance] icon in the

[Menu] screen.
The [Maintenance] menu screen appears.

2 Touch the [Rinse Instrument] icon.

The [Rinse Instrument] dialog box appears.

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5 Insert the pipette all the way to the 6 Remove CELLCLEAN AUTO from the
bottom of the CELLCLEAN AUTO, and pipette.
press the start switch. Remove CELLCLEAN AUTO without bending the
pipette.

7 Wait until cleaning finishes.

When the process finishes, the dialog box closes, and
the instrument power automatically turns OFF.

CELLCLEAN AUTO Start switch

Aspiration starts.
During aspiration, the analysis status indicator LED
blinks green and the beeper sounds repeatedly.
When aspiration finishes, the analysis status indicator
LED turns off and the beeping stops. Cleaning starts.

Replace air pump.

Probable cause Actions

It is time to replace the air pump. Touch [Accept] in the [Help] dialog box.
Replace the air pump. For details, see the following.

Replacing the air pump 3 Touch the [Menu] button on the toolbar.
The [Menu] screen appears.

4 Touch the [Shutdown] icon.

Caution, Hot! The [Shutdown] dialog box appears.
The surface of the air pump is hot. After turning OFF
the main power, make sure that the air pump has 5 Touch [OK].
cooled sufficiently before replacing it. The instrument power automatically turns OFF.

Air pump that is used

Part number Item name

05104711 Air pump set No. 1

1 Shut down the instrument.

1 Make sure the instrument is in the
ready state.
If the instrument status at the bottom left of the
screen is not green, wait until it becomes green.

2 Remove the sample tube from the

sample tube holder.
(XN-550/XN-450 only)

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2 Turn OFF the main power switch, and 4 Remove the screws (x2) with a Phillips
unplug the power cable. head screwdriver, and remove the upper
cover.

Upper cover

Main power
ON switch

OFF

Plug

Screws (x2)

Example: XN-550

3 Open the right cover.

Release the lock by turning to the left with a flathead
screwdriver, and open the right cover.

Right cover

Example: XN-550

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5 Loosen the screws (x2) with a Phillips 7 Remove the connectors and tubes (x2),
head screwdriver. and remove the air pump unit.
Screws (x2) Tube (P1)

Connector

Tube (V1)

8 Connect the connectors and tubes (x2)

to the new air pump.
See the illustration of step 7.

Information
Connect the tube (P1) to the upper side.

9 Place the air pump unit in the

instrument, and fasten with the screws
Example: XN-550 (x2) with a Phillips head screwdriver.

10 Attach the upper cover, and fasten with

Caution! the screws (x2) with a Phillips head
Take care not to drop the screws. screwdriver.
It may be very difficult to remove a screw that falls into
the instrument.
11 Close the right cover and lock.
Turn the lock to the right with a flathead screwdriver.
6 Pull up the air pump unit.
12 Turn ON the main power switch.

13 Press the power switch.

Start the instrument and log on.

14 Reset the counter of the air pump to "0".

Display the [Unit] tab of the [Counter] dialog box, and
reset the count.
For details on the [Unit] tab and the reset procedure,
see the following.
Air pump unit (➤P.82 "2.10 Checking the cycle count (counter)")

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CELLPACK DCL has expired, CELLPACK DFL has expired,

SULFOLYSER has expired, Lysercell WDF has expired

Probable cause Actions

The reagent has expired. Touch [Execute] in the [Help] dialog box.
The [Reagent Replacement] dialog box appears. Replace the
reagent. For details, see the following.

Replacing the diluent and hemolytic When the reagent code is entered,
the reagent in the dialog box shows
agent [Received]. The lot number and
expiration date show the
1 Prepare a new reagent. information of the new reagent.
Make sure the reagent has not expired.

Caution! Note:
• Place the reagent container at a level no more than
When inputting manually, touch the name of the
1 meter above or below the bottom of the analyzer.
reagent to be replaced in the [Reagent Replacement]
Do not place reagents on top of the instrument.
dialog box. The dialog box below appears.
• The new reagent must be allowed to sit for at least
24 hours at room temperature (15 to 35°C) before
use.
• If reagent spills, immediately wipe up the spill
using a moistened cloth.

2 Touch [Execute] in the [Help] dialog

box.
The [Reagent Replacement] dialog box appears.
(➤P.84 "Chapter 2: 2.12.1 [Reagent Replacement]
dialog box")

3 Input the reagent code (barcode) of the Touch [Replace the reagent.] to select the checkbox,
new reagent. enter the [Reagent Code :], and touch [OK].
Input by reading the barcode
Scan the reagent code (barcode) on the outer box of
the new reagent with the hand-held barcode reader. 4 Remove the cap from the new reagent
The reagent code is as shown below.
container.
Make sure the barcode scanning surface is flat before
scanning.
5 Remove the cap from the old reagent
container.

Note:
In case the reagent outer box label shows a "XN
Reagent Code" or "Reagent Code 2" barcode, Example: Lysercell WDF (2 L)
please scan this barcode.

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6 Pull the spout set straight up and out. 7 Insert the spout set straight into the new
reagent container and close the cap.

8 Touch [Execute].
Reagent replacement starts. Wait until this process
finishes. When replacement finishes, the [Reagent
Replacement] dialog box closes.

Time guidelines for reagent replacement are shown

below.

Caution! Reagent name Time

Do not touch the aspiration nozzle on the spout set. CELLPACK DCL Approx. 1 minute
Take care that dust does not get on the spout set.
CELLPACK DFL Approx. 3 1/2 minutes
SULFOLYSER Approx. 2 1/2 minutes
Lysercell WDF Approx. 3 1/2 minutes

Fluorocell WDF has expired, Fluorocell RET has expired

Probable cause Actions

The reagent has expired. Replace the reagent. For details, see the following.

Replacing the dye

2 Prepare a new reagent cartridge.
Make sure the reagent has not expired.
Caution!
While performing replacement, always wear
adequate personal protective equipment.

1 Touch [Execute] in the [Help] dialog

box.
The [Reagent Replacement] dialog box appears.
Example: Fluorocell WDF
(➤P.84 "Chapter 2: 2.12.1 [Reagent Replacement]
dialog box")

When touching [Close] in the [Help] dialog box:

Follow the steps below to display the [Reagent
Replacement] dialog box.

1 Touch the [Maintenance] icon in the

[Menu] screen.
The [Maintenance] menu screen appears.

2 Touch the [Exchange] icon.

The [Exchange] dialog box appears.

3 Touch [Reagent Replacement].

The [Reagent Replacement] dialog box appears.

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3 Pull out the dye holder. 5 Slowly remove the dye cover, taking
care that the dye does not drip.
Dye holder

Dye cover

Caution!
Dye may drip from the nozzle on the dye cover.
If dye drips onto the instrument, immediately wipe it off
using a moistened cloth. Otherwise, the coated surface
of the instrument may become discolored.

6 Remove the old reagent cartridge from

the holder.

Example: XN-550

4 Remove the entire dye holder.

Old reagent cartridge
Fluorocell WDF

7 Insert the new reagent cartridge in the

holder.

Fluorocell RET

Holder
New reagent cartridge

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8 Place the dye cover. 9 Place the holder in the dye holder.

10 Close the dye holder.

Dye cover The information on the RFID tag of the cartridge is
read, and if normal, a beep sounds. The lot number
and expiration date show the information of the new
reagent.

Information
Be sure to push the dye holder all the way in.

Press Start SW

Probable cause Actions

This appears when the instrument is in the sleep state. Press the start switch. The fluid path is rinsed and the
instrument wakes up from the sleep state.
On the XN-550, when the instrument is in sampler analysis
mode, it can also be woken from the sleep state by pressing
the sampler start/stop switch.

Errors related to the dye holder

Wrong reagent installed in Fluorocell WDF holder, Wrong reagent installed in Fluorocell RET holder

Probable cause Actions

An incorrect dye type has been installed. Remove the incorrect reagent, wipe the nozzle of the dye
holder, and then install the correct reagent.
Dispose of the incorrect reagent.

Fluorocell WDF is not installed, Fluorocell RET is not installed

Probable cause Actions

The dye is not installed. Install the reagent. For details, see the following.
(➤P.61 "Replacing the dye")

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Fluorocell WDF has already been used, Fluorocell RET has already been used

Probable cause Actions

A used dye has been installed. Replace the reagent. For details, see the following.

Replacing the dye

3 Pull out the dye holder.

Caution! Dye holder

While performing replacement, always wear
adequate personal protective equipment.

1 Touch [Execute] in the [Help] dialog

box.
The [Reagent Replacement] dialog box appears.
(➤P.84 "Chapter 2: 2.12.1 [Reagent Replacement]
dialog box")

When touching [Close] in the [Help] dialog box:

Follow the steps below to display the [Reagent
Replacement] dialog box.

1 Touch the [Maintenance] icon in the

[Menu] screen.
The [Maintenance] menu screen appears.

2 Touch the [Exchange] icon.

The [Exchange] dialog box appears.

3 Touch [Reagent Replacement].

The [Reagent Replacement] dialog box appears.

2 Prepare a new reagent cartridge.

Make sure the reagent has not expired.
Example: XN-550

4 Remove the entire dye holder.

Fluorocell WDF

Example: Fluorocell WDF

Fluorocell RET

Holder

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5 Slowly remove the dye cover, taking 8 Place the dye cover.
care that the dye does not drip.

Dye cover

Dye cover

9 Place the holder in the dye holder.

Caution!
Dye may drip from the nozzle on the dye cover. 10 Close the dye holder.
If dye drips onto the instrument, immediately wipe it off The information on the RFID tag of the cartridge is
using a moistened cloth. Otherwise, the coated surface read, and if normal, a beep sounds. The lot number
of the instrument may become discolored.
and expiration date show the information of the new
reagent.

6 Remove the old reagent cartridge from

the holder.
Information
Be sure to push the dye holder all the way in.

Old reagent cartridge

7 Insert the new reagent cartridge in the

holder.

New reagent cartridge

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Cannot recognize Fluorocell WDF information, Cannot recognize Fluorocell RET information

Probable cause Actions

The RFID tag on the reagent cartridge is damaged. Touch [Execute] in the [Help] dialog box.
The [Reagent Replacement] dialog box appears. Touch the
error dye and register the reagent information in the dialog
box that appears. For details, see the following.

Registering reagent information

3 After checking or entering the lot
number, touch [Accept].
1 Touch [Execute] in the [Help] dialog If the lot number displayed in the dialog box is invalid,
box. touch the [Lot No.] field and enter the correct lot
The [Reagent Replacement] dialog number.
box appears. The error dye shows
After [Accept] is touched, a dialog box appears for
[Exchange], as shown on the right.
confirmation of the serial number.

Note:
[Accept] is enabled 5 seconds after the dialog box
2 Touch the dye that indicates appears. If the lot number is invalid and appears in
[Exchange]. red, [Accept] will not be enabled.

The dialog box for lot number confirmation appears.

4 Continue to check or enter the

displayed parameters and touch
[Accept].
Complete the procedure with entering the address of
the manufacturer or vendor and touch [Register]. The
dialog box closes and [Exchange] changes to
[Received].
A

[Reagent Replacement] (lot number confirmation)

dialog box
One of the messages below will appear (A) above to
indicate whether the lot number was properly read
from the RFID tag.
• Normal: [Check the contents.]
• Error: [Reagent information read from tag is
invalid.]

[Reagent Replacement] dialog box

5 Touch [Execute] in the [Reagent

Replacement] dialog box.
Reagent replacement starts.

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Fluorocell WDF RFID tag error, Fluorocell RET RFID tag error

Probable cause Actions

Unable to write the information on the RFID tag of the reagent Replace the reagent (a reagent with a normal RFID tag). For
cartridge. details, see the following.

Replacing the dye

3 Pull out the dye holder.

Caution! Dye holder

While performing replacement, always wear
adequate personal protective equipment.

1 Touch [Execute] in the [Help] dialog

box.
The [Reagent Replacement] dialog box appears.
(➤P.84 "Chapter 2: 2.12.1 [Reagent Replacement]
dialog box")

When touching [Close] in the [Help] dialog box:

Follow the steps below to display the [Reagent
Replacement] dialog box.

1 Touch the [Maintenance] icon in the

[Menu] screen.
The [Maintenance] menu screen appears.

2 Touch the [Exchange] icon.

The [Exchange] dialog box appears.

3 Touch [Reagent Replacement].

The [Reagent Replacement] dialog box appears.

2 Prepare a new reagent cartridge.

Make sure the reagent has not expired.
Example: XN-550

4 Remove the entire dye holder.

Fluorocell WDF

Example: Fluorocell WDF

Fluorocell RET

Holder

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5 Slowly remove the dye cover, taking 8 Place the dye cover.
care that the dye does not drip.

Dye cover

Dye cover

9 Place the holder in the dye holder.

Caution!
Dye may drip from the nozzle on the dye cover. 10 Close the dye holder.
If dye drips onto the instrument, immediately wipe it off The information on the RFID tag of the cartridge is
using a moistened cloth. Otherwise, the coated surface read, and if normal, a beep sounds. The lot number
of the instrument may become discolored.
and expiration date show the information of the new
reagent.

6 Remove the old reagent cartridge from

the holder.
Information
Be sure to push the dye holder all the way in.

Old reagent cartridge

7 Insert the new reagent cartridge in the

holder.

New reagent cartridge

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Errors related to the sampler analysis operation

Sampler cover (front) is open.

Probable cause Actions

The sampler cover (front) is open. Close the sampler cover (front).

Sampler cover (front) is opened.

Probable cause Actions

The sampler cover (front) opened while the sampler analysis Remove the sample tubes from the instrument. When the
was in operation. hand is gripping a sample tube, open the sampler cover (front
or manual unit) and remove the sample tubes. Close the
sampler cover (front and manual unit) and then touch
[Execute] in the [Help] dialog box. The sampler test is
executed.
If an abnormality occurs during the operation test, the sensor
that detects the cover has failed. Contact your Sysmex service
representative.
Sampler analysis cannot be performed, but manual analysis is
possible.

Sampler cover (manual unit) is open.

Probable cause Actions

The sample cover (manual unit) is open. Close the sample cover (manual unit).

Sampler cover (manual unit) is opened.

Probable cause Actions

The sampler cover (manual unit) opened while the sampler Remove the sample tubes from the instrument. When the
analysis was in operation. hand is gripping a sample tube, open the sampler cover (front
or manual unit) and remove the sample tubes. Close the
sampler cover (front and manual unit) and then touch
[Execute] in the [Help] dialog box. The sampler test is
executed.
If an abnormality occurs during the operation test, the sensor
that detects the cover has failed. Contact your Sysmex service
representative.
Sampler analysis cannot be performed, but manual analysis is
possible.

Sampler Adapter holder is open.

Probable cause Actions

The sampler adapter holder (right)/(left) is open. Close the sampler adapter holder (right)/(left).

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Sampler Adapter holder is opened.

Probable cause Actions

The sampler adapter holder (right)/(left) opened while the Remove the sample tubes from the instrument. When the
sampler analysis was in operation. hand is gripping a sample tube, open the sampler cover (front
or manual unit) and remove the sample tubes. Close the
sampler cover (front and manual unit) and then touch
[Execute] in the [Help] dialog box. The sampler test is
executed.
If an abnormality occurs during the operation test, the sensor
that detects the cover has failed. Contact your Sysmex service
representative.
Sampler analysis cannot be performed, but manual analysis is
possible.

Sampler adapter is not placed

Probable cause Actions

Sampler adapter is not placed. Place the sampler adapter and press the sampler start/stop
switch.

Cannot recognize sampler adapter

Probable cause Actions

An unregistered sampler adapter was placed. Place an appropriate sampler adapter, and then execute
The sampler adapter is damaged. sampler analysis.
If the error persists, execute manual analysis, or execute
sampler analysis using another sampler adapter.

Errors related to the hand and sample tube holders

Hand F-B motor error, Hand L-R motor error, Hand up-down error, Hand open/close error, Mixing error

Probable cause Actions

The hand did not work properly. Remove the sample tubes from the instrument. When the hand
is gripping a sample tube, open the sampler cover (front or
manual unit) and remove the sample tubes. Close the sampler
cover (front and manual unit) and then touch [Execute] in the
[Help] dialog box. The sampler test is executed.
If an abnormality occurs during the operation test, the hand
has failed. Contact your Sysmex service representative.
Sampler analysis cannot be performed, but manual analysis is
possible. Before executing manual analysis, check if the hand
is touching the sample tube holder. If so, move the hand.

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Chapter 2 Maintenance

This chapter describes the procedures for maintenance, replacing and replenishing supplies, and checking
instrument operation.

2.1 List of maintenance tasks

To maintain optimum instrument performance, periodic maintenance must be executed.
When using the instrument on a continuous basis, execute "Daily maintenance" once every 24 hours.
Execute maintenance according to the schedule below. Record the maintenance tasks that are executed in the
maintenance and inspection checklist. (➤P.100 "2.19 Maintenance and inspection checklist")

Risk of infection
While working, always wear adequate personal protective equipment, such as protective gloves, a
protective mask, protective eyewear, and a lab coat. Wash your hands after completing the task.
There is a risk of infection.

Maintenance task Pages

Daily Shutdown ➤Basic Operation, "Chapter
maintenance 1: 1.3 Shutdown"
Weekly Executing routine cleaning XN-550 ➤P.74
maintenance XN-450 ➤P.75
XN-350 ➤P.76
Maintenance Replacing the air pump ➤P.89
once every 2 For product codes, see "General Information".
years (➤General Information, "Chapter 6: 6.1 Supplies")
As needed Replenishing the reagent ➤P.77
Replacing the waste container ➤P.78
Replacing the reagent ➤P.84
Replacing the piercer (XN-550/XN-450) ➤P.88
Replacing the fuse ➤P.92
For product codes, see "General Information".
(➤General Information, "Chapter 6: 6.1 Supplies")
Cleaning the aspiration unit tray XN-550 ➤P.94
XN-450 ➤P.94
XN-350 ➤P.96
Adjusting the monitor image quality (XN-550) ➤P.98

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2.2 Displaying maintenance screens

Maintenance tasks, checking of operation, and operation tests are executed in the maintenance screens that are
opened from the [Maintenance] menu screen.

1 Make sure the instrument is in the ready

state.
If the instrument status at the bottom left of the
screen is not green, wait until it becomes green.

2 Touch the [Maintenance] icon in the

[Menu] screen.
The [Maintenance] menu screen appears.

[Maintenance] menu screen

3 Touch the icon of the desired

maintenance category.
The maintenance item menu dialog box appears.

Example: [Operation Confirmation] dialog box

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2.3 Maintenance screens

Touch the [Maintenance] icon in the [Menu] screen to display the [Maintenance] menu screen.
Open the menu dialog box of the desired maintenance items from the [Maintenance] menu screen.

● [Maintenance] menu screen ● [Operation Confirmation] dialog box

[Maintenance] menu screen [Operation Confirmation] dialog box

[Operation Opens the [Operation [Whole Blood Touch to start the whole blood
Confirmation] Confirmation] dialog box. Select Aspiration Motor aspiration motor test.
the unit you want to check. Test] (➤P.79 "2.9.1 Motor test")
(➤P.71 "● [Operation
[Sheath Motor Touch to start the sheath motor
Confirmation] dialog box")
Test] test.
[Exchange] Opens the [Exchange] dialog (➤P.79 "2.9.1 Motor test")
box. Select the item you want to
[Aspiration Unit Touch to start the aspiration unit
replace.
Motor Test] motor test.
(➤P.72 "● [Exchange] dialog
(➤P.79 "2.9.1 Motor test")
box")
[Air Pump Test] Touch to start the air pump test.
[Rinse Opens the [Rinse Instrument]
(➤P.79 "2.9.2 Air pump test")
Instrument] dialog box. Select the unit to be
rinsed and the rinsing method. [Pinch Valve Touch to start the pinch valve test.
(➤P.72 "● [Rinse Instrument] Test] (➤P.80 "2.9.3 Pinch valve test")
dialog box") [Tube Holder Touch to start the sample tube
[Status Opens the [Status Confirmation] Motor Test]*1 holder motor test.
Confirmation] dialog box. Select the (➤P.79 "2.9.1 Motor test")
information (analysis or cycle [Barcode Use to test the barcode reader in
count/unit status) you want to Reading Test]*2 the instrument.
check. (➤P.80 "2.9.4 Barcode reader
(➤P.73 "● [Status Confirmation] reading test (XN-550)")
dialog box")
[Sampler Touch to start the sampler test.
[Back] Touch to return to the [Menu] Operation Test]*2 (➤P.81 "2.9.5 Sampler operation
screen. test (XN-550)")
[Close] The dialog box closes.

*1 Does not appear on the XN-350.

*2 XN-550 only.

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● [Exchange] dialog box [Rinse Flowcell] Touch to rinse the flowcell.

XN-550
(➤P.37 "Chapter 1: Rinsing the
flowcell (XN-550)")
XN-450
(➤P.38 "Chapter 1: Rinsing the
flowcell (XN-450)")
XN-350
(➤P.39 "Chapter 1: Rinsing the
flowcell (XN-350)")
[Drain Reaction Touch to drain the sample that has
Chamber] collected in the reaction chamber.

[Exchange] dialog box [Drain RBC Touch to drain the sample that has
Isolation collected in the RBC isolation
[Reagent Touch to display the [Reagent Chamber] chamber.
Replacement] Replacement] dialog box. Use this (➤P.34 "Chapter 1: Draining the
dialog box to replace a reagent. sample from the RBC isolation
(➤P.84 "2.12 Replacing the chamber")
reagent") [Remove RBC Touch to remove a clog from the
[Close] The dialog box closes. Detector Clog] RBC detector.
(➤P.34 "Chapter 1: Removing the
RBC detector clog")
● [Rinse Instrument] dialog box
[Routine Touch to execute routine cleaning.
Cleaning] XN-550
(➤P.74 "2.4 Executing routine
cleaning (XN-550)")
XN-450
(➤P.75 "2.5 Executing routine
cleaning (XN-450)")
XN-350
(➤P.76 "2.6 Executing routine
cleaning (XN-350)")
[Reagent Use to replace the reagent in the
Replenishment] fluid line.
[Rinse Instrument] dialog box (➤P.77 "2.7 Replenishing the
reagent")
[Drain Waste Touch to drain the waste fluid that [Close] The dialog box closes.
Fluid Chamber] has collected in the waste fluid
chamber.
[Rinse Waste Touch to rinse the waste fluid
Fluid Chamber] chamber.
XN-550
(➤P.20 "Chapter 1: Rinsing the
waste chamber (XN-550)")
XN-450
(➤P.21 "Chapter 1: Rinsing the
waste chamber (XN-450)")
XN-350
(➤P.22 "Chapter 1: Rinsing the
waste chamber (XN-350)")
[Remove Touch to remove bubbles from the
Flowcell Air flowcell.
Bubbles] (➤P.46 "Chapter 1: Removing air
bubbles from the flowcell")

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● [Status Confirmation] dialog box

[Status Confirmation] dialog box

[Counter] Use to check the analysis count

and cycle count of each unit.
(➤P.82 "2.10 Checking the cycle
count (counter)")
[Sensor] Use to check the status of each
unit.
(➤P.83 "2.11 Checking the
instrument status (sensor)")
[Close] The dialog box closes.

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2.4 Executing routine cleaning (XN-550)

The routine cleaning must be executed once every 1,000 analyses, or once a week. Routine cleaning consists of
aspirating CELLCLEAN AUTO and rinsing the fluid line.

1 Touch the [Maintenance] icon in the 5 Place CELLCLEAN AUTO in the sample
[Menu] screen. tube holder.
The [Maintenance] menu screen appears. Place in the regular sample tube holder.

2 Touch the [Rinse Instrument] icon. CELLCLEAN

The [Rinse Instrument] dialog box appears. AUTO

3 Touch [Routine Cleaning].

The [Routine Cleaning] dialog box appears. Regular sample
tube holder

[Routine Cleaning] dialog box

6 Press the start switch.

4 Open the sampler cover (manual unit).

The sample tube holder retracts into the instrument

and aspiration starts. When aspiration finishes,
cleaning starts and the sample tube holder is ejected.

7 Remove CELLCLEAN AUTO.

8 Close the sampler cover (manual unit).

9 Wait until cleaning finishes.

When the process finishes, the dialog box closes, and
the instrument power automatically turns OFF.

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2.5 Executing routine cleaning (XN-450)

The routine cleaning must be executed once every 1,000 analyses, or once a week. Routine cleaning consists of
aspirating CELLCLEAN AUTO and rinsing the fluid line.

1 Touch the [Maintenance] icon in the 5 Place CELLCLEAN AUTO in the sample
[Menu] screen. tube holder.
The [Maintenance] menu screen appears. Place in the regular sample tube holder.

2 Touch the [Rinse Instrument] icon.

The [Rinse Instrument] dialog box appears.

3 Touch [Routine Cleaning]. CELLCLEAN

The [Routine Cleaning] dialog box appears. AUTO

Regular
sample tube
holder

6 Press the start switch.

[Routine Cleaning] dialog box

4 If the sample tube holder is not ejected,

press the sample tube holder open/close
switch.
The sample tube holder is ejected.
The sample tube holder retracts into the instrument
and aspiration starts. When aspiration finishes,
cleaning starts and the sample tube holder is ejected.

7 Remove CELLCLEAN AUTO.

8 Wait until cleaning finishes.

When the process finishes, the dialog box closes, and
the instrument power automatically turns OFF.

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2.6 Executing routine cleaning (XN-350)

The routine cleaning must be executed once every 1,000 analyses, or once a week. Routine cleaning consists of
aspirating CELLCLEAN AUTO and rinsing the fluid line.

1 Touch the [Maintenance] icon in the 5 Insert the pipette all the way to the
[Menu] screen. bottom of the CELLCLEAN AUTO, and
The [Maintenance] menu screen appears. press the start switch.

2 Touch the [Rinse Instrument] icon.

The [Rinse Instrument] dialog box appears.

3 Touch [Routine Cleaning].

The [Routine Cleaning] dialog box appears.

CELLCLEAN AUTO Start switch

Aspiration starts.
During aspiration, the analysis status indicator LED
blinks green and the beeper sounds repeatedly.
When aspiration finishes, the analysis status indicator
LED turns off and the beeping stops. Cleaning starts.
[Routine Cleaning] dialog box

6 Remove CELLCLEAN AUTO from the

4 Open CELLCLEAN AUTO with the pipette.
special CELLCLEAN AUTO opener.
Remove CELLCLEAN AUTO without bending the
With CELLCLEAN AUTO held straight as shown,
pipette.
press the opener down until you hear a “pop” sound.

7 Wait until cleaning finishes.

Opener When the process finishes, the dialog box closes, and
the instrument power automatically turns OFF.

Keep the opener attached, and remove immediately

before you use CELLCLEAN AUTO.

Caution!
• Always wear adequate personal protective
equipment when opening CELLCLEAN AUTO.
• Press down slowly so that the fluid does not spill.
• Store opened CELLCLEAN AUTO standing on the
rack with the opener attached. If CELLCLEAN
AUTO is tilted, the fluid may leak even with the
opener attached.

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2.7 Replenishing the reagent

If an incorrect reagent is accidentally connected, the reagent in the fluid line can be replenished.

1 Make sure that the correct reagent is

connected.

2 Touch the [Maintenance] icon in the

[Menu] screen.
The [Maintenance] menu screen appears.

3 Touch the [Rinse Instrument] icon.

The [Rinse Instrument] dialog box appears.

4 Touch [Reagent Replenishment].

The [Reagent Replenishment] dialog box appears.
The content that appears depends on your system
configuration.

[Reagent Replenishment] dialog box

5 Select the checkbox of the reagent you

want to replenish.
Touch the reagent name to select or unselect the
checkbox.
The selected reagent will be replenished.

6 Touch [OK].
Reagent replenishing starts.
Wait until this process finishes. When the process
finishes, the dialog box closes.

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2.8 Replacing the waste container

When a waste container is installed, this procedure is used to replace the waste container. When the waste container
becomes full, replace the waste container.
If you are using a waste container full sensor and the waste container becomes full, [Waste container is full] appears
in the [Help] dialog box. Promptly replace the waste container.
Follow the procedure below to replace the waste container when using a waste container full sensor.

6 Touch [Accept] in the [Help] dialog box.

Risk of infection
Take care that waste fluid does not splash.
Caution!
Install the waste container below the bottom of the
1 Prepare an empty waste container and instrument.
remove the cap.

2 Turn the cap on the full waste container

in the direction of the arrow to loosen.

Waste container

3 Lift the cap with the tube connected

straight up.
Dispose of the full waste container in accordance with
local laws and regulations.

4 Insert the cap with the tube connected

straight into the new waste container.

5 Close the cap by turning it in the

direction opposite to that of step 2.

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2.9 Testing instrument operation

The operation of each unit can be tested. These procedures can be used to identify the cause of an error.
To execute an operation test, the instrument must be in the ready state. Operation tests cannot be executed in any
other state. Analysis is not possible while an operation test is in progress.

2.9.1 Motor test 2.9.2 Air pump test

The following motor operations can be tested. Activate the air pump and execute the operation test.

Name Operation
Whole blood Aspirates and discharges
1 Touch the [Maintenance] icon in the
aspiration motor samples. [Menu] screen.
The [Maintenance] menu screen appears.
Sheath motor Feeds reaction sample to the
FCM detector or RBC/PLT
detector. 2 Touch the [Operation Confirmation]
Aspiration unit motor Moves the piercer (XN-550/ icon.
XN-450) or pipette (XN-350) The [Operation Confirmation] dialog box appears.
that aspirates the sample.
Sample tube holder Ejects and retracts the 3 Touch [Air Pump Test].
motor* sample tube holder.
The operation test starts, and the [Air Pump Test]
* XN-550/XN-450 only.
dialog box appears.

1 Touch the [Maintenance] icon in the

[Menu] screen.
The [Maintenance] menu screen appears.

2 Touch the [Operation Confirmation]

icon.
The [Operation Confirmation] dialog box appears. Displays the current pressure value.
[Air Pump Test] dialog box
3 Touch the motor test button. Wait until this process finishes. When the process
finishes, the dialog box closes.
The operation test starts, and the dialog box appears.
If an error occurred during the test, an error message
appears.

Example: [Whole Blood Aspiration Motor Test]

dialog box
Wait until this process finishes. When the process
finishes, the dialog box closes.
If an error occurred during the test, an error message
appears.

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2.9.3 Pinch valve test

4 Touch [Barcode Reading Test].
There is a pinch valve in the waste chamber and in the The [Barcode Reading Test] dialog box appears.
rinse cup aspiration unit.

1 Touch the [Maintenance] icon in the

[Menu] screen.
The [Maintenance] menu screen appears.

2 Touch the [Operation Confirmation] icon.

The [Operation Confirmation] dialog box appears.

3 Touch the [Pinch Valve Test] icon.

The operation test starts, and the [Pinch Valve Test]
dialog box appears.

Reading result
[Barcode Reading Test] dialog box

[Sample No.] Displays the sample number that

was read from the barcode.
[CD] Displays the check digit of the
[Pinch Valve Test] dialog box barcode.
Wait until this process finishes. When the process [Type] Displays the type of barcode.
finishes, the dialog box closes. Reading result Displays the read result. One of
If an error occurred during the test, an error message the following symbols is
appears. displayed, depending on the
result. If there was no problem
2.9.4 Barcode reader reading test with reading, nothing is
displayed.
(XN-550) [E]: Reading error or invalid
check digit.
When a barcode read error occurs, execute the barcode
[+]: A value that is longer than
reader reading test to identify the cause of the error.
the specified number of
digits was read.
1 If the button at the right edge of the [-]: A value that is shorter than
control menu is [Manual], press the the specified number of
digits was read.
mode switch.
Change to sampler analysis mode.

5 Pull out the sampler adapter holder

2 Touch the [Maintenance] icon in the (left), and remove the sampler adapter.
[Menu] screen.
The [Maintenance] menu screen appears. 6 Place a sample tube with a barcode
label in the sampler adapter.
3 Touch the [Operation Confirmation]
icon. 7 Place the sampler adapter in the
The [Operation Confirmation] dialog box appears. sampler adapter holder (left).

8 Push in the sampler adapter holder (left).

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9 Touch [Start]. 4 Touch [Sampler Operation Test].

The reading test starts. The operation test starts, and the [Sampler Operation
If a previous test result was displayed, it is cleared Test] dialog box appears.
when the test starts.
Wait until this process finishes. When reading finishes,
the result is displayed.

10 Touch [Cancel].
The dialog box closes.

[Sampler Operation Test] dialog box

11 Pull out the sampler adapter holder
(left), and remove the sampler adapter. Wait until this process finishes. When the process
finishes, the dialog box closes.
If an error occurred during the test, an error message
12 Remove the sample tube from the appears.
sampler adapter.

13 Place the sampler adapter in the

sampler adapter holder (left).

14 Push in the sampler adapter holder

(left).
15

2.9.5 Sampler operation test

(XN-550)
The sampler operation test is used to test the sequence
of operation from removing a sample tube from the
sampler adapter and mixing the sample to replacing the
sample tube in the sample tube holder, and reverse
operation.

1 If the button at the right edge of the

control menu is [Manual], press the
mode switch.
Change to sampler analysis mode.

2 Touch the [Maintenance] icon in the

[Menu] screen.
The [Maintenance] menu screen appears.

3 Touch the [Operation Confirmation]

icon.
The [Operation Confirmation] dialog box appears.

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2.10 Checking the cycle count (counter)

Use to check the analysis count of each analysis mode and the cycle count of each unit.

[Pump] tab
1 Touch the [Maintenance] icon in the
[Menu] screen.
The [Maintenance] menu screen appears.

2 Touch the [Status Confirmation] icon.

The [Status Confirmation] dialog box appears.

3 Touch [Counter].
The [Counter] dialog box appears.

[Counter] dialog box ([Pump] tab)

[Pump Counter] Displays the cycle count of each

pump.

[Unit] tab

[Counter] dialog box ([Measurement] tab)

Select the tabs to check the analysis count, pump

cycle count, and counts of other units.

[Measurement] tab

[Measurement Displays the analysis count of

Mode Counter] each analysis mode. [Counter] dialog box ([Unit] tab)
[Maintenance] shows the count
of background check (including [Unit Counter] Displays the cycle count of each
background checks for body unit.
fluid analysis). [Cleaning(Cycle, Day)] shows
the analysis count and the
[Test Counter] Displays the analysis count of
number of days elapsed since
each discrete and test.
the last time routine cleaning
[Total] sums up the [Repeat],
was executed.
[Rerun], and [Reflex] counts.
[Piercer] appears when the
On models other than the
instrument is the XN-550 or
XN-550, there is no function for
XN-450.
Repeat analysis, Rerun analysis,
or Reflex analysis, and thus this [Air Pump Reset] Resets the air pump counter to
field is blank. 0.

4 Touch [Close].
The dialog box closes.

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2.11 Checking the instrument status (sensor)

You can verify the temperature, pressure, and sensor status of each unit. The information displayed is updated every
0.5 seconds.
You can also execute analysis with the [Sensor Display] dialog box open and showing the information.

1 Touch the [Maintenance] icon in the 4 Touch [Close].

[Menu] screen. The dialog box closes.
The [Maintenance] menu screen appears.

2 Touch the [Status Confirmation] icon.

The [Status Confirmation] dialog box appears.

3 Touch [Sensor].
The [Sensor Display] dialog box appears.
Sensor status display area

[Sensor Display] dialog box

[Pressure Displays the pressure of each

(units: MPa)] unit.
[Temperature Displays the temperature of
(units: °C)] each unit in the instrument and
the ambient temperature.
[HGB] Displays the conversion value
for hemoglobin. Nothing is
displayed during analysis.
[Aspiration Displays the conversion value
Sensor] for the aspiration sensor.
Nothing is displayed during
analysis.
[Laser Current] Displays the output current of
the laser.
Sensor status Displays the status of each
display area sensor.
Red: ON
White: OFF
The [Sampler] sensor status is
only displayed on the XN-550.

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2.12 Replacing the reagent

When the replacement of reagent is required, the error message appears. Promptly replace the indicated reagent.

2.12.1 [Reagent Replacement] Lot No. Displays the lot number of the
dialog box reagent.
Reagent state Displays the remaining number
The [Reagent Replacement] dialog box allows you to of tests that can be executed
check how much reagent remains and replace reagents. with the reagent.
For CELLPACK DCL, the
remaining volume appears.
1 Touch the [Maintenance] icon in the The remaining number of tests is
[Menu] screen. an approximate value. The value
The [Maintenance] menu screen appears. will vary depending on
conditions of use. The value is
2 Touch the [Exchange] icon. not displayed if the reagent has
not been registered. When the
The [Exchange] dialog box appears.
reagent runs low, the
background becomes yellow.
3 Touch [Reagent Replacement]. During diluent or hemolytic
agent replacement, progress is
The [Reagent Replacement] dialog box appears.
indicated as "0 to 100%".
Reagent name Displays the reagent name.
Remaining The remaining volume of the
volume graph reagent is shown as a graph.
This is not displayed if the
reagent has not been registered,
or if the reagent has run out.

Note:
The [Reagent Replacement] dialog box can also be
displayed by touching the remaining reagent display
in the control menu.
In addition, touching [Execute] in the [Help] dialog box
when a reagent replacement error message appears
also displays the [Reagent Replacement] dialog box.
In this case, only the reagent for which the error
[Reagent Replacement] dialog box occurred can be replaced.

The dialog box shows information on each reagent

and how much remains.
Shelf life after opening

Lot No.
Remaining volume
graph
Reagent state
Reagent name

Shelf life after Displays the shelf life of the

opening reagent after opening.
The value is not displayed if the
reagent has not been registered.
When the shelf life after opening
has expired, it appears in white
on a red background.

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2.12.2 Replacing the diluent and

hemolytic agent
Note:
When the replacement of reagent such as CELLPACK
When inputting manually, touch the name of the
DCL, CELLPACK DST, SULFOLYSER, Lysercell WDF, or reagent to be replaced in the [Reagent Replacement]
CELLPACK DFL is required, follow the steps below to dialog box. The dialog box below appears.
replace it.

1 Prepare a new reagent.

Make sure the reagent has not expired.

Caution!
• Place the reagent container at a level no more than
1 meter above or below the bottom of the analyzer.
Do not place reagents on top of the instrument.
• The new reagent must be allowed to sit for at least
24 hours at room temperature (15 to 35°C)* before
use.
Touch [Replace the reagent.] to select the checkbox,
• If reagent spills, immediately wipe up the spill
enter the [Reagent Code :], and touch [OK].
using a moistened cloth.
* Use CELLPACK DST at 15 to 30°C.

4 Remove the cap from the new reagent

2 Display the [Reagent Replacement] container.
dialog box.
(➤P.84 "2.12.1 [Reagent Replacement] dialog box")
5 Remove the cap from the old reagent
3 Input the reagent code (barcode) of the container.
new reagent.
Input by reading the barcode
Scan the reagent code (barcode) on the outer box of
the new reagent with the hand-held barcode reader.
The reagent code is as shown below.
Make sure the barcode scanning surface is flat before
scanning.

Example: Lysercell WDF (2 L)

6 Pull the spout set straight up and out.

Note:
In case the reagent outer box label shows a "XN
Reagent Code" or "Reagent Code 2" barcode,
please scan this barcode.

When the reagent code is entered,

the reagent in the dialog box shows
[Received]. The lot number and
Caution!
Do not touch the aspiration nozzle on the spout set.
expiration date show the
Take care that dust does not get on the spout set.
information of the new reagent.

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7 Insert the spout set straight into the new 3 Pull out the dye holder.
reagent container and close the cap.
Dye holder
8 Touch [Execute].
Reagent replacement starts. Wait until this process
finishes. When replacement finishes, the [Reagent
Replacement] dialog box closes.

Time guidelines for reagent replacement are shown

below.

Reagent name Time

CELLPACK DCL Approx. 1 minute
CELLPACK DFL Approx. 3 1/2 minutes
SULFOLYSER Approx. 2 1/2 minutes
Lysercell WDF Approx. 3 1/2 minutes

2.12.3 Replacing the dye

When the replacement of reagent such as Fluorocell
WDF or Fluorocell RET is required, follow the steps
below to replace it.

Caution!
While performing replacement, always wear
adequate personal protective equipment.
Example: XN-550

1 Display the [Reagent Replacement] 4 Remove the entire dye holder.

dialog box.
(➤P.84 "2.12.1 [Reagent Replacement] dialog box") Fluorocell WDF

2 Prepare a new reagent cartridge.

Make sure the reagent has not expired.

Fluorocell RET

Example: Fluorocell WDF Holder

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5 Slowly remove the dye cover, taking 8 Place the dye cover.
care that the dye does not drip.

Dye cover

Dye cover

9 Place the holder in the dye holder.

Caution!
Dye may drip from the nozzle on the dye cover. 10 Close the dye holder.
If dye drips onto the instrument, immediately wipe it off The information on the RFID tag of the cartridge is
using a moistened cloth. Otherwise, the coated surface read, and if normal, a beep sounds. The lot number
of the instrument may become discolored.
and expiration date show the information of the new
reagent.

6 Remove the old reagent cartridge from

the holder.
Information
Be sure to push the dye holder all the way in.

Old reagent cartridge

7 Insert the new reagent cartridge in the

holder.

New reagent cartridge

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2.13 Replacing the piercer (XN-550/XN-450)

When the piercer operation count exceeds 120,000, [Piercer replacement is required.] appears in the [Help] dialog
box. Contact your local Sysmex representative to request a piercer replacement immediately. A Sysmex technical
representative will replace the part.
Continuing to use the old piercer will cause tip wear and may result in problems such as blood aspiration related
errors, compromised data integrity, or needle breakage.

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2.14 Replacing the air pump

Caution, Hot!
The surface of the air pump is hot. After turning OFF the main power, make sure that the air pump has
cooled sufficiently before replacing it.

Air pump that is used

Part number Item name

05104711 Air pump set No. 1

The air pump must be replaced once every 30,000 analyses, or once every 2 years. Continuing to use the old air
pump may result in breakage or other problems.
When [Replace air pump.] appears, promptly replace the air pump.
A replacement air pump is not included with the instrument. Contact your local Sysmex representative to purchase a
replacement air pump.

1 Shut down the instrument. 2 Turn OFF the main power switch, and
unplug the power cable.
1 Make sure the instrument is in the
ready state.
If the instrument status at the bottom left of the
screen is not green, wait until it becomes green.

2 Remove the sample tube from the

sample tube holder. Main power
ON
(XN-550/XN-450 only) switch

3 Touch the [Menu] button on the toolbar. OFF

The [Menu] screen appears.

Plug
4 Touch the [Shutdown] icon.
The [Shutdown] dialog box appears.

5 Touch [OK].
The instrument power automatically turns OFF.

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3 Open the right cover. 5 Loosen the screws (x2) with a Phillips
Release the lock by turning to the left with a flathead head screwdriver.
screwdriver, and open the right cover.
Screws (x2)

Right cover

Example: XN-550

4 Remove the screws (x2) with a Phillips

head screwdriver, and remove the upper
cover.

Upper cover
Example: XN-550

Caution!
Take care not to drop the screws.
It may be very difficult to remove a screw that falls into
the instrument.

6 Pull up the air pump unit.

Screws (x2)

Example: XN-550

Air pump unit

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7 Remove the connectors and tubes (x2),

and remove the air pump unit.

Tube (P1)

Connector

Tube (V1)

8 Connect the connectors and tubes (x2)

to the new air pump.
See the illustration of step 7.

Information
Connect the tube (P1) to the upper side.

9 Place the air pump unit in the

instrument, and fasten with the screws
(x2) with a Phillips head screwdriver.

10 Attach the upper cover, and fasten with

the screws (x2) with a Phillips head
screwdriver.

11 Close the right cover and lock.

Turn the lock to the right with a flathead screwdriver.

12 Turn ON the main power switch.

13 Press the power switch.

Start the instrument and log on.

14 Reset the counter of the air pump to "0".

Display the [Unit] tab of the [Counter] dialog box, and
reset the count.
For details on the [Unit] tab and the reset procedure,
see the following.
(➤P.82 "2.10 Checking the cycle count (counter)")

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2.15 Replacing the fuse

If a fuse blows, replace the fuse.

Warning!
• Always unplug the power cable before replacing a fuse.
An electrical shock could occur.
• Use only a fuse of the specified type and rating.
Doing so could cause a smoke emission.

Fuse that is used

Part number Item name Rating Type Number of fuse

26677681 Fuse 50T100H 250 V 10 A Time lag 2

1 Turn OFF the main power switch, and 2 Remove the old fuse.
unplug the power cable.
1 Lift the tab on the fuse holder with a
flathead screwdriver and pull out the
fuse holder.

Fuse holder

Main power Tab

ON switch

OFF

Plug

2 Remove the old fuse from the fuse

holder.

Fuse

Fuse holder

3 Place the new fuse in the fuse holder

and insert the fuse holder into the
instrument.

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4 Plug in the power cable.

5 Turn ON the main power switch.

6 Press the power switch.

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2.16 Cleaning the aspiration unit tray (XN-550/XN-450)

If the aspiration unit tray becomes dirty, clean the aspiration unit tray.

Risk of infection
Always wear adequate personal protective equipment, such as protective gloves, a protective mask,
protective eyewear, and a lab coat when cleaning the aspiration unit tray. Wash your hands after
completing the task.
There is a risk of infection if the tray is contaminated with blood or other material.

1 Shut down the instrument. 3 Open the right cover.

Release the lock by turning to the left with a flathead
1 Make sure the instrument is in the screwdriver, and open the right cover.
ready state.
If the instrument status at the bottom left of the
screen is not green, wait until it becomes green.

2 Remove the sample tube from the

sample tube holder.

3 Touch the [Menu] button on the toolbar.

The [Menu] screen appears.

4 Touch the [Shutdown] icon.

The [Shutdown] dialog box appears.

5 Touch [OK].
The instrument power automatically turns OFF.

2 Turn OFF the main power switch. Right cover

Example: XN-550

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4 Remove the screw (x1) with a Phillips 7 Make sure no dirt remains, and wipe off
head screwdriver. the water.
Screw
8 Install the aspiration unit tray in the
instrument, and fasten with the screw
(x1) with a Phillips head screwdriver.
See the illustrations of steps 4 and 5.

9 Close the right cover and lock.

Turn the lock to the right with a flathead screwdriver.

10 Turn ON the main power switch.

11 Press the power switch.

Start the instrument and log on.

Example: XN-550

Caution!
Take care not to drop the screws you removed.
It may be very difficult to remove a screw that falls
into the instrument.

5 Remove the aspiration unit tray.

Aspiration unit tray

6 Clean the aspiration unit tray using tap

water or alcohol.

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2.17 Cleaning the aspiration unit tray (XN-350)

If the aspiration unit tray becomes dirty, clean the aspiration unit tray.

Risk of infection
Always wear adequate personal protective equipment, such as protective gloves, a protective mask,
protective eyewear, and a lab coat when cleaning the aspiration unit tray. Wash your hands after
completing the task.
There is a risk of infection if the tray is contaminated with blood or other material.

1 Shut down the instrument. 3 Open the right cover.

Release the lock by turning to the left with a flathead
1 Make sure the instrument is in the screwdriver, and open the right cover.
ready state.
If the instrument status at the bottom left of the
screen is not green, wait until it becomes green.

2 Touch the [Menu] button on the toolbar.

The [Menu] screen appears.

3 Touch the [Shutdown] icon.

The [Shutdown] dialog box appears.

4 Touch [OK].
The instrument power automatically turns OFF.

2 Turn OFF the main power switch.

Right cover

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4 Remove the aspiration unit tray.

Aspiration unit tray

5 Clean the aspiration unit tray using tap

water or alcohol.

6 Make sure no dirt remains, and wipe off

the water.

7 Install the aspiration unit tray in the

instrument.
See the illustration of step 4.

Information
Install so that the protrusion on the aspiration unit
tray is on the right side.

8 Close the right cover and lock.

Turn the lock to the right with a flathead screwdriver.

9 Turn ON the main power switch.

10 Press the power switch.

Start the instrument and log on.

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2.18 Adjusting the monitor image quality (XN-550)

When you want to change the monitor image (brightness, color, position, etc.), adjust the image with the OSD
(on-screen display) buttons.

OSD (on-screen display) buttons

2.18.1 Displaying the OSD screen 2.18.2 Closing the OSD screen

1 Press the MENU button. 1 Press the EXIT button.

The OSD screen (page 1) The OSD screen closes.
appears on the monitor.
To display page 2 (Option
Setting), press the  or  Note:
button to move the cursor
• The OSD screen can be closed by moving the
(gray background) to
cursor to [Cancel] in the OSD screen (page 1) and
[Option] and press the pressing the MENU button.
MENU button. • If the OSD buttons are not used for more than 10
seconds when changing settings, the settings are
automatically saved and the OSD screen closes.
This time can be set using [OSD Timeout].

2.18.3 Changing the monitor settings

Page 1

1 Move the cursor to the desired setting item.

To return to page 1, press Press the  or  button to move the cursor (gray
the  or  button to move background).
the cursor to [Cancel] and For a description of each item, see the following.
press the MENU button. (➤P.99 "2.18.4 Setting items and functions in the
OSD screen")

2 Press the MENU button.

The item is selected and the cursor background
changes to yellow.
When an item that does not have settings
([AutoAdjust], [Save Data], etc.) is selected, the
function of the item is executed.

Page 2
3 Change the setting.
• To increase a value, press the  button.
• To decrease a value, press the  button.

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4 Press the MENU button. 5 Move the cursor to [Save Data].

The item is unselected and the cursor background
changes to gray. 6 Press the MENU button.
The settings are saved.

2.18.4 Setting items and functions in the OSD screen

Setting item/
Pages Description Setting range Default setting
function
1 Contrast Adjustment of contrast of all RGB colors 0 to 255 55
H Position Horizontal position adjustment 28 to 228 128
V Position Vertical position adjustment 105 to 151* 128
HTotal (Clock) Horizontal total count setting 64 to 192 128
Phase Phase setting (flickering adjustment) 0 to 63 0
Contrast Red Red contrast adjustment (digital setting) 0 to 255 50
Contrast Green Green contrast adjustment (digital setting) 0 to 255 50
Contrast Blue Blue contrast adjustment (digital setting) 0 to 255 50
Backlight Brightness adjustment 0 to 31 31
AutoAdjust Auto position adjustment -
AutoColor Auto color adjustment -
Default Initial setting -
Save Data Data save -
Cancel Cancel or OSD screen closing -
Option Display of page 2 -
2 Red Gain Red ADC gain adjustment (analog setting) 0 to 255 128
Green Gain Green ADC gain adjustment (analog setting) 0 to 255 128
Blue Gain Blue ADC gain adjustment (analog setting) 0 to 255 128
Red Offset Red ADC offset adjustment (analog setting) 0 to 255 128
Green Offset Green ADC offset adjustment (analog setting) 0 to 255 128
Blue Offset Blue ADC offset adjustment (analog setting) 0 to 255 128
Filter Space filter setting Non Non
Gamma Gamma value setting 0.1 to 3.0 1.0
OSD HPosition OSD horizontal position adjustment 3 to 255 128
OSD VPosition OSD vertical position adjustment 41 to 211 128
OSD Effect OSD transparency effect setting 0 to 7 0
OSD Timeout Timeout setting for lights-out of OSD Non, 5 to 10, 15, 10
20, 30, 40, 50, 60
Default Initial setting -
Save Data Data save -
Cancel Display of page 1 -

* Value when SVGA 60 Hz is input. The range varies depending on the input resolution and vertical frequency.

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2.19
Chapter 2

Daily maintenance tasks Month Yr.

Day
Maintenance task
Shutdown
Maintenance

Signed

Weekly maintenance tasks

Maintenance task M/D Signed M/D Signed M/D Signed M/D Signed M/D Signed M/D Signed M/D Signed M/D Signed

Routine cleaning

Maintenance tasks once every 2 years Reagents and supply parts replacement
Maintenance task M/D Signed M/D Signed Maintenance task M/D Signed M/D Signed

Replace the air pump Replace the reagent (CELLPACK DCL)

Replace the reagent (CELLPACK DST)
Maintenance tasks to be performed as needed Replace the reagent (CELLPACK DFL)
Maintenance task M/D Signed M/D Signed Replace the reagent (SULFOLYSER)
Replenish the reagent Replace the reagent (Lysercell WDF)
Maintenance and inspection checklist

Replace the waste container Replace the reagent (Fluorocell WDF)

Adjust the monitor image quality Replace the reagent (Fluorocell RET)
Clean the aspiration unit tray Replace the air pump
Replace the fuse

* We recommend that our customers prepare a checklist that suits their operating environment.

XN-L series XN-550/XN-450/XN-350 Troubleshooting

 Chapter 3 Calibration

Chapter 3 Calibration

3.1 Overview of calibration

Calibration is performed to maintain the accuracy of the system. Calibration compensates variances of the air
pressure system, fluid system and electrical system, which affect the accuracy of analysis results.

Calibrator calibration
A special calibrator is used to calibrate the instrument.
Manually analyze the same calibrator 11 times, and check the repeatability and accuracy of the analysis
parameters.
At the same time, the compensation rate can be updated.

Precision check
Check the instrument's repeatability using a normal sample.

Note:
• Before performing calibration, check the level of the reagent connected to the instrument.
If the reagent runs out, calibration will stop.
• The following sample numbers are automatically assigned by the analyzer.
• Calibrator calibration: CAL-CAL-01 to CAL-CAL-11
• Precision check: PRE-CHK-01 to PRE-CHK-11

3.1.1 Calibration standards

The initial calibration is done by your Sysmex technical representative, at the time of installation.
Perform calibration when needed; for example, when the QC data fluctuates. However, if the abnormality in the
QC analysis data is caused by an error in the analyzer, degradation of the reagent, or degeneration of the
control blood, do not perform calibration.

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3.1.2 Calibrators and samples to be used

Use the following calibrators and samples for calibrator calibration and precision check.

● Calibrator calibration
XN CAL: use for WBC, RBC, HGB, HCT, PLT, and RET calibration.

● Precision check
For a precision check, use fresh normal blood that meets the following requirements.
• Blood of a healthy person who is not taking any medicine
• Blood with an appropriate amount of anticoagulant added
• Whole blood volume of 2.0 mL or more per sample

Information
Control blood is not suitable for calibrator calibration. Control blood is intended for quality control, not for
calibration.

3.1.3 Main calibration functions

The instrument supports the following calibration functions.

Functions Description Pages

Print Calibration results (log) can be output to the printer. ➤P.113
CSV output Calibration results (log) can be output to a file in CSV format. ➤P.114
Save Calibration results (log) can be saved. (Backup) ➤P.115
Restore Calibration results (log) can be restored. (Restore) ➤P.115
Delete A calibration log can be deleted. ➤P.116

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3.2 Performing calibrator calibration

Follow the steps below to perform a calibrator calibration.

1 Make sure the instrument is in the ready 3 Touch the [Calibrator Calibration] icon.
state. The [Calibrator Calibration] (analysis) dialog box
If the instrument status at the bottom left of the appears.
screen is not green, wait until it becomes green.
Calibration parameters

2 Touch the [Calibration] icon in the

[Menu] screen.
The [Calibration] menu screen appears.

[Calibrator Calibration] (analysis) dialog box

Calibration The analysis parameters to be

[Calibration] menu screen
parameters calibrated appear. The parameters
that appear depend on your system
configuration.
[No. 1] to For each calibration parameter, the
[No. 11] analysis results of 11 analysis cycles
appear.
The results of [No. 1] are shown with
a strike-through because they are
not included in [Mean Value], [SD],
and [CV (%)].
[Mean Value] For each calibration parameter, the
mean value of the analyzed values
from [No. 2] to [No. 11] appears.
[SD] For each calibration parameter, the
standard deviation for the analyzed
values from [No. 2] to [No. 11] appears.
[CV (%)] The coefficient of variation of the
analysis results of each calibration
parameter appears. After the 11th
analysis is completed, if the
coefficient of variation is greater than
the [Limit (%)], it appears in white
characters on a red background.
[Limit (%)] The standard value (allowable value)
for the coefficient of variation of each
calibration parameter appears.
[Switch Display] Touch to display the details. Touch
again to close the details.
[Calibration] Touch to display the [Calibrator
Calibration] (confirmation) dialog box.

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3 Press the start switch.

4 Mix the vial containing the calibrator as
shown.
For details on the mixing method, see the package
insert for the calibrator.

The sample tube holder retracts into the instrument

and manual analysis starts.
When aspiration finishes, the sample tube holder is
ejected.

5 Execute manual analysis. 4 Repeat mixing and analysis (total of

11 times).

Note: Information
• Calibrator calibration is performed using manual If an error occurs during analysis, stop calibrator
analysis. calibration. After clearing the error, repeat the
• The discrete parameters to be analyzed are procedure from the beginning.
specified automatically, and cannot be changed.
• If you have the RET channel analysis license:
CBC+DIFF+RET
• If you do not have the RET channel analysis XN-450:
license:
CBC+DIFF
1 If the sample tube holder is not ejected,
press the sample tube holder open/
XN-550: close switch.
The sample tube holder is ejected.
1 Open the sampler cover (manual unit).
2 Place the vial in the sample tube holder.
Place in the regular sample tube holder.

Vial

Regular sample
tube holder

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2 Place the vial in the sample tube holder. XN-350:

Place in the regular sample tube holder.
1 Open the vial cap.
When removing the cap, take care that the calibrator
does not spill.

2 Insert the pipette all the way to the

bottom of the vial, and press the start
Vial switch.

Regular sample
tube holder

3 Press the start switch.

Vial Start switch

When analysis finishes, the result is shown in [No. 1]

in the [Calibrator Calibration] (analysis) dialog box.

3 Repeat mixing and analysis (total of

The sample tube holder retracts into the instrument 11 times).
and manual analysis starts.
When aspiration finishes, the sample tube holder is
ejected.
Information
4 Repeat mixing and analysis (total of If an error occurs during analysis, stop calibrator
calibration. After clearing the error, repeat the
11 times). procedure from the beginning.

Information 6 If you need to repeat analysis, select the

If an error occurs during analysis, stop calibrator
analysis number and repeat the manual
calibration. After clearing the error, repeat the analysis.
procedure from the beginning. The results from the analysis in step 5 appear in the
[Calibrator Calibration] (analysis) dialog box.
If the analysis results do not meet the following
requirements, the analysis must be repeated.
• All analysis results are normal.
• All calibration parameters are below the [Limit (%)]
value.
When the analysis results meet the requirements,
[Calibration] in the [Calibrator Calibration] (analysis)
dialog box can be touched. Proceed to the next step.

7 Remove the vial. (XN-550/XN-450 only)

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[Delta Percent When the target value is entered, a

8 Touch [Calibration]. (%)] value that is equal to "|Target value -
The [Calibrator Calibration] (confirmation) dialog box Mean Value|/Mean Value x 100 (%)" is
appears. displayed.
If this value is greater than the
Acceptable Limit and less than the
Service Limit, the background is
displayed in yellow. If the value is
greater than the Service Limit, the
value is displayed in white characters
on a red background.
[Acceptable Displays a numeric value for
Limit (%)] determining whether calibration is
necessary. If the Delta Percent is less
than this value, no calibration is
necessary.
[Service Limit Displays the maximum value of the
Data display area (%)] Delta Percent when performing
calibrator calibration. If the Delta
[Calibrator Calibration] (confirmation)
Percent is greater than this value,
dialog box
calibration cannot be performed for
that parameter.
[Analysis Result] Touch to display the [Calibrator
[Current Rate Displays the compensation rate for
Calibration] (analysis) dialog box.
(%)] each calibration parameter before
[Lot No.] The lot number can be entered. calibrator calibration.
Up to 8 characters can be entered.
[New Rate (%)] Displays the new compensation rate,
Double-byte characters cannot be
which is calculated from "Target value
entered.
x Current Rate/Mean Value". The rate
[Read] This is used if the target values of appears when [Target] and [Mean
calibration parameters are imported Value] are entered.
from a server or a memory medium
with a saved display value file.
Touch after entering the [Lot No.] to
read in the target values.
Data display area
[Target] Referring to the target sheet supplied
with the XN CAL, manually enter the
target value of each calibration
parameter.
• You can also use [Lot No.] and
[Read] to read in the values from a
server or a memory medium with a
saved display value file.
[Range Value] Displays the difference between the
maximum and the minimum values for
each calibration parameter. If the
difference is greater than the
maximum range value, it is displayed
in white characters on a red
background.
[Max Range] When the target value is entered, a
value that is equal to "Target value x
Fixed ratio for each calibration
parameter" is displayed.
[Mean Value] Displays the average value of the
analysis data.

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9 Touch [OK]. Calibrator Select a checkbox to include that

The [Calibrator Calibration] (execute) dialog box parameter calibration parameter in the
checkboxes calibrator calibration. Clear a
appears.
checkbox to exclude that parameter
The parameters that appear depend on your system from calibrator calibration.
configuration. If a calibration parameter meets all
of the conditions below, the
checkbox for that parameter is
automatically selected when the
screen appears. In addition, you can
select or clear checkboxes
manually.
1) 80% ≤ New Compensation Rate
≤ 120%
2) New Compensation Rate -
Current Compensation Rate ≤
±5%
3) Range Value ≤ Max Range
4) Acceptable Limit ≤ Delta Percent
≤ Service Limit

If a calibration parameter meets all

Calibrator parameter Modify checkbox
checkboxes
of conditions 1) to 3) and the Delta
Percent is less than the Acceptable
[Calibrator Calibration] (execute) dialog box Limit, the parameter is excluded
from calibration, as there is no need
for calibration.
If a calibration parameter does not
meet any of conditions 1) to 3) and
the Delta Percent is greater than the
Acceptable Limit, calibration cannot
be performed. Calibration is
performed with this calibration
parameter excluded.
[Current Displays the compensation rate for
Compensation each calibration parameter before
Rate (%)] calibrator calibration.
[New Displays the new compensation rate
Compensation calculated by the system.
Rate (%)]
Modify You can select a checkbox to
checkbox manually enter a value in [New
Compensation Rate (%)]. A value
within the range 80 to 120% can be
entered. However, a checkbox
cannot be selected for a calibration
parameter with "Delta Percent >
Acceptable Limit". Calibration
parameters with manually entered
values will be displayed with an
asterisk [*] next to its value in the
calibrator calibration log.
When a checkmark is removed, a
value cannot be entered in [New
Compensation Rate (%)]. If a value
was manually entered prior to
removing the checkmark, the value
will revert to the system-calculated
value.
[Back] Touch to display the previous screen.
[Next] Touch to display the next screen.

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10 Touch [Next].
The following dialog box appears.
The parameters that appear depend on your system
configuration.

Calibrator parameter
checkboxes Modify checkbox

[Calibrator Calibration] (execute) dialog box

11 Touch [OK].
The compensation rate is updated.

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3.3 Performing precision check

Follow the steps below to perform a precision check.

1 Make sure the instrument is in the ready 3 Touch the [Precision Check] icon.
state. The [Precision Check] (analysis) dialog box appears.
If the instrument status at the bottom left of the Precision check parameters
screen is not green, wait until it becomes green.

2 Touch the [Calibration] icon in the

[Menu] screen.
The [Calibration] menu screen appears.

[Precision Check] (analysis) dialog box

Precision check The analysis parameters to be

parameters analyzed in the precision check
[Calibration] menu screen appear. The parameters that
appear depend on your system
configuration.
[No. 1] to For each precision check
[No. 11] parameter, the analysis results
of 11 analysis cycles appear.
The results of [No. 1] are shown
with a strike-through because
they are not included in [Mean
Value], [SD], and [CV (%)].
[Mean Value] For each precision check
parameter, the mean value of
the analyzed values from [No. 2]
to [No. 11] appears.
[SD] For each precision check
parameter, the standard
deviation for the analyzed values
from [No. 2] to [No. 11] appears.
[CV (%)] The coefficient of variation of the
analysis results of each
precision check parameter
appears. After the 11th analysis
is completed, if the coefficient of
variation is greater than the
[Limit (%)], it appears in white
characters on a red background.
[Limit (%)] The standard value (allowable
value) for the coefficient of
variation of each precision check
parameter appears.
[Switch Display] Touch to display the details.
Touch again to close the details.

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3 Press the start switch.

4 Mix the vial containing the sample as
shown.

The sample tube holder retracts into the instrument

and manual analysis starts.
When analysis finishes, the sample tube holder is
5 Execute manual analysis. ejected.

4 Repeat mixing and analysis (total of

11 times).
Note:
• The precision check is performed using manual
analysis. Information
• The discrete parameters to be analyzed are If an error occurs during analysis, stop precision
specified automatically, and cannot be changed. check. After clearing the error, repeat the procedure
• If you have the RET channel analysis license: from the beginning.
CBC+DIFF+RET
• If you do not have the RET channel analysis
license:
CBC+DIFF XN-450:

XN-550:
1 If the sample tube holder is not ejected,
press the sample tube holder open/
1 Open the sampler cover (manual unit). close switch.
The sample tube holder is ejected.
2 Place the vial in the sample tube holder.
Place in the regular sample tube holder.

Vial

Regular sample
tube holder

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2 Place the vial in the sample tube holder. XN-350:

Place in the regular sample tube holder.
1 Open the vial cap.
When removing the cap, take care that the sample
does not spill.

2 Insert the pipette all the way to the

bottom of the vial, and press the start
Vial switch.

Regular sample
tube holder

3 Press the start switch.

Vial Start switch

When analysis finishes, the result is shown in [No. 1]

in the [Precision Check] (analysis) dialog box.

3 Repeat mixing and analysis (total of

The sample tube holder retracts into the instrument 11 times).
and manual analysis starts.
When analysis finishes, the sample tube holder is
ejected.
Information
4 Repeat mixing and analysis (total of If an error occurs during analysis, stop precision
11 times). check. After clearing the error, repeat the procedure
from the beginning.

Information 6 If you need to repeat analysis, select the

If an error occurs during analysis, stop precision
analysis number and repeat the manual
check. After clearing the error, repeat the procedure
from the beginning. analysis.
The results from the analysis in step 5 appear in the
[Precision Check] (analysis) dialog box.
If the analysis results do not meet the following
requirements, the analysis must be repeated.
• All analysis results are normal.
• All calibration parameters are below the [Limit (%)]
value.
When the analysis results meet the requirements,
[OK] in the [Precision Check] (analysis) dialog box can
be touched. Proceed to the next step.

7 Remove the vial. (XN-550/XN-450 only)

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8 Touch [OK].
The following dialog box appears.

9 Touch [Yes].
The result of the precision check is recorded.

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3.4 Checking the calibration log/precision check log

You can view the logs of the saved calibrator calibration and precision check.

Note:
Up to 20 logs of each type are saved. Once 20 logs are saved, each new log automatically deletes the
oldest log.

3.4.1 Checking the calibration log/

3 Check the log.
precision check log
For details, see the following.
Follow the steps below to check a log. (➤P.117 "3.5 [Calibration History] screen")
(➤P.119 "3.6 [Precision Check History] screen")
1 Touch the [Calibration] icon in the
[Menu] screen.
The [Calibration] menu screen appears. 3.4.2 Printing the calibration log/
precision check log on a printer
You can print a log on the printer.
Follow the steps below to print a log.

1 Display the log screen you want to print.

(➤P.113 "3.4.1 Checking the calibration log/
precision check log")

2 Select the log you want to print.

[Calibration] menu screen

2 Touch the [Calibration History]/

[Precision Check History] icon.
The [Calibration History]/[Precision Check History]
screen appears.

Example: [Calibration History] screen

3 Touch the [Output] button -

[Ledger (LP)] on the toolbar.
The log is output to the printer.

Example: [Calibration History] screen

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3.4.3 Saving the calibration log/

5 Remove the USB memory stick.
precision check log in CSV
format Save directory and file name
When you save a log, the log is saved using the file name
You can save a log to a USB memory stick as a file in
below in a folder* that has the instrument ID as the folder
CSV format.
name.
Follow the steps below to save a log in CSV format.
Calibrator calibration log:
Instrument ID_Software version_CALIBRATOR_CAL_
1 Display the desired log screen. Execution date_Execution time.csv
(➤P.113 "3.4.1 Checking the calibration log/ Precision check log:
precision check log") Instrument ID_Software version_PRECISION_CHECK_
Execution date_Execution time.csv

2 Insert the USB memory stick.

Example: XN-XXX_00-01_PRECISION_CHECK_
Insert the USB memory stick into a free USB port on 20140505_080808.csv
the back of the instrument.
A USB memory stick with a password lock function * If a folder with the instrument ID as the folder name
cannot be used. does not exist in the USB memory stick, the folder is
automatically created.

USB memory
stick
USB port

3 Select the log you want to save.

4 Touch the [File] button - [Output in CSV

Format] on the toolbar.
The data save starts and the [Waiting to complete
execution] dialog box appears.
The [Waiting to complete execution] dialog box closes
when the data save is complete.

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3.4.4 Saving the calibration log/

5 Remove the USB memory stick.
precision check log
(backup) Backup directory and file name
When you back up a log, the log is saved using the file
Calibration log data can be backed up to a USB memory
name below in a folder* that has the instrument ID as the
stick.
folder name.
Follow the steps below to save a log.
Calibrator calibration log:
[Instrument ID][Software version][Execution date_
1 Display the desired log screen. Execution time].cad
(➤P.113 "3.4.1 Checking the calibration log/ Precision check log:
precision check log") [Instrument ID][Software version][Execution date_
Execution time].pre

2 Insert the USB memory stick.

Example: [XN-XXX][00-01][20140505_080808].pre
Insert the USB memory stick into a free USB port on
the back of the instrument. * If a folder with the instrument ID as the folder name
A USB memory stick with a password lock function does not exist in the USB memory stick, the folder is
cannot be used. automatically created.

3.4.5 Restoring a saved calibration

log/precision check log
(restore)
USB memory
stick You can restore saved log data.
USB port

Note:
If the number of logs exceeds 20 during restoring,
the oldest log is automatically deleted.

Follow the steps below to restore a log.

1 Display the desired log screen.

(➤P.113 "3.4.1 Checking the calibration log/
precision check log")

3 Select the log you want to save.

Select by touching the cursor move buttons to move
the cursor.

4 Touch the [File] button - [Backup] on the

toolbar.
The backup starts and the [Waiting to complete
execution] dialog box appears.
The [Waiting to complete execution] dialog box closes
when the backup is complete.

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2 Insert the USB memory stick that 6 Remove the USB memory stick.
contains the backup log file.
Insert the USB memory stick into a free USB port on
3.4.6 Deleting a calibration log/
the back of the instrument.
A USB memory stick with a password lock function
precision check log
cannot be used.
Follow the steps below to delete a log.

1 Display the desired log screen.

(➤P.113 "3.4.1 Checking the calibration log/
precision check log")

USB memory
stick 2 Select the log you want to delete.
USB port

3 Touch the [Delete] button on the toolbar.

The following dialog box appears.

3 Touch the [File] button - [Restore] on the

toolbar. 4 Touch [Yes].
The [Import] dialog box appears. The dialog box closes, and the log is deleted.

[Import] dialog box

4 Select the checkbox of the file you want

to restore.
Files with checkmarks will be restored.
Each time you touch the checkbox, the checkbox is
selected or unselected.

5 Touch [OK].
The restore starts and the [Waiting to complete
execution] dialog box appears.
The [Waiting to complete execution] dialog box closes
when the restore is complete.

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3.5 [Calibration History] screen

Toolbar

Log list

[Calibration History] screen

Toolbar Displays the function buttons.

[Details] Touch to show the analysis results of the selected calibration log in a dialog box.

[Output] Touch to open a submenu that allows you to output the selected log.
(➤P.113 "3.4.2 Printing the calibration log/precision check log on a printer")

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[File] Touch to open a submenu that allows you to save and restore the log selected in
the list.
[Output in CSV Format]: Use to save a log in CSV format.
(➤P.114 "3.4.3 Saving the calibration log/precision check
log in CSV format")
[Backup]: Use to back up a log.
(➤P.115 "3.4.4 Saving the calibration log/precision check
log (backup)")
[Restore]: Use to restore the backed up data.
(➤P.115 "3.4.5 Restoring a saved calibration log/
precision check log (restore)")
[Delete] Use to delete the log selected in the list.
(➤P.116 "3.4.6 Deleting a calibration log/precision check log")
Log list Displays the calibration logs in the list.
[Calibration Date] Displays the date and time when the calibration was performed.
[Logon Name] Displays the name of the user who was logged on when the calibration was
performed.
[Material] Displays the name of the calibrator.
[Lot No.] Displays the lot number of the calibrator.
Compensation rate Displays the compensation rate of each analysis parameter.
When a new compensation rate has been manually input for a parameter, [*]
appears in front of the compensation rate.

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3.6 [Precision Check History] screen

Toolbar

Log list

[Precision Check History] screen

Toolbar Displays the function buttons.

[Details] Touch to show the analysis results of the selected precision check log in a dialog
box.

[Output] Touch to open a submenu that allows you to output the selected log.
(➤P.113 "3.4.2 Printing the calibration log/precision check log on a printer")

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[File] Touch to open a submenu that allows you to save and restore the log selected in
the list.
[Output in CSV Format]: Use to save a log in CSV format.
(➤P.114 "3.4.3 Saving the calibration log/precision check
log in CSV format")
[Backup]: Use to back up a log.
(➤P.115 "3.4.4 Saving the calibration log/precision check
log (backup)")
[Restore]: Use to restore the backed up data.
(➤P.115 "3.4.5 Restoring a saved calibration log/
precision check log (restore)")
[Delete] Use to delete the log selected in the list.
(➤P.116 "3.4.6 Deleting a calibration log/precision check log")
Log list Displays the precision check logs.
[Execution Date] Displays the date and time when the precision check was performed.
[Logon Name] Displays the name of the user who was logged on at the time of the precision
check.
Analysis results Displays the CV (%) of each precision check parameter.

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Chapter 4 Checking Logs

4.1 Logs
You can check the following logs that are saved on the instrument.
• [Audit Log]: Instrument operation log
• [Error Log]: Errors that occurred, and information on when the errors occurred and when they
were cleared
• [Maintenance Log]: Maintenance tasks executed, and information at the time of execution
• [Reagent Replacement Log]: Reagents replaced, and information entered at the time of replacement

Note:
• Up to 5,000 logs of each type are saved. Once 5,000 logs are saved, each new log automatically deletes
the oldest log.
• The calibration logs (calibrator calibration and precision check) cannot be viewed from the [History] menu
screen. For details on calibration logs, see Chapter 3.
(➤P.113 "Chapter 3: 3.4 Checking the calibration log/precision check log")

4.1.1 Checking a log 4.1.2 Adding a comment to a log

Follow the steps below to check a log. You can add a comment to a log.

1 Touch the [History] icon in the [Menu]

screen. Information
The [History] menu screen appears. Once a comment is entered, it cannot be edited or
deleted. If a comment is entered when a comment
already exists, the new comment is added after the
previous comment.

Follow the steps below to enter a comment.

1 Display the screen of the log you want

to add a comment to.
(➤P.121 "4.1.1 Checking a log")

[History] menu screen

2 Touch the button of the log you want to

view.
The selected log screen appears.

3 Check the log.

For details, see the following.
(➤P.124 "4.2 Log screen")

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4.1.4 Saving a log in CSV format

2 Select the log you want to add a
comment to. A log list can be saved to a USB memory stick as a file in
CSV format.
Follow the steps below to save a log in CSV format.

1 Display the desired log screen.

(➤P.121 "4.1.1 Checking a log")

2 Insert the USB memory stick.

Insert the USB memory stick into a free USB port on
the back of the instrument.
A USB memory stick with a password lock function
cannot be used.
Example: [Audit Log] screen

3 Touch the [Input] button on the toolbar.

The software keyboard appears.

4 Enter a comment. USB memory

stick
You can enter up to 50 characters. USB port

5 Touch [OK].
The comment is added.

4.1.3 Printing a log on a printer

A log list can be output to a printer.
Follow the steps below to print a log.

1 Display the log screen you want to print.

(➤P.121 "4.1.1 Checking a log")
3 Touch the [File] button - [Output in CSV
2 Touch the [Output] button - [Ledger (LP)] Format] on the toolbar.
on the toolbar. The data save starts and the [Waiting to complete
The log is output to the printer. execution] dialog box appears.
The [Waiting to complete execution] dialog box closes
when the data save is complete.

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4 Remove the USB memory stick.

Save directory and file name

When you save a log, the log is saved using the file name
below in a folder* that has the instrument ID as the folder
name.
Audit log:
Instrument ID_Software version_AUDITLOG.csv
Error log:
Instrument ID_Software version_ERRORLOG.csv
Maintenance log:
Instrument ID_Software version_MAINTENANCELOG.csv
Reagent replacement log:
Instrument ID_Software version_REAGENTLOG.csv

Example: XN-XXX_00-01_REAGENTLOG.csv

* If a folder with the instrument ID as the folder name

does not exist in the USB memory stick, the folder is
automatically created.

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4.2 Log screen

Toolbar

Details button

Log list

Details table

Example: [Audit Log] screen

Toolbar Displays the function buttons.

[Input] Touch to display a software keyboard that allows you to enter a comment.
(➤P.121 "4.1.2 Adding a comment to a log")
[Output] Touch to open a submenu that allows you to output the log list.
(➤P.122 "4.1.3 Printing a log on a printer")
[File] Touch to open a submenu that allows you to save the log list.
(➤P.122 "4.1.4 Saving a log in CSV format")
Log list Displays a list of the logs.
Details button Touch to display the details of the log selected in the log list below the log list.
Touch again to close the details.

4.2.1 Contents of the log list and details

● [Audit Log] screen

[Date] Displays the date and time the log was registered.
[Logon Name] Displays the name of the user that was logged on when the log was registered.
[Operation Name] Displays the name of the operation that was performed.
[Details]* Displays the details of the operation that was performed.
[Comments]* Displays the entered comments.

* When the Details button is touched, the details appear below the log list.

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Operation names and display conditions are shown below.

Operation names Display conditions

[Logon] When a user logs on.
[Logoff] When a user logs off.
[Service Logon] When a service technician logs on.
[Service Logoff] When a service technician logs off.
[Modify Sample No.] When the sample number of analysis data is modified.
[Modify Pos. -> Neg.] When the judgment of analysis data is changed from Positive to Negative.
[Modify Neg. -> Pos.] When the judgment of analysis data is changed from Negative to Positive.
[Modify Sample Inf.] When the attributes of sample number of analysis data is modified.
[Modify Patient ID] When the patient ID of analysis data is modified.
[Delete Analysis Data] When analysis data is deleted*1.
[Register QC File] When a QC file is registered.
[Modify QC Lot] When QC lot attributes (expiration date and lot number) are changed.
[Modify QC Target/Limit] When a QC target is changed.
[Delete QC File] When a QC file is deleted.
[Delete QC Plot] When a QC plot is deleted.
[Delete Analysis Registration] When an analysis order is deleted*1.
[Execute Calibration] When a compensation rate is changed.
[Change Settings] When settings are changed.
[Restore Settings] When saved settings are restored.
[Initialize Settings] When settings are initialized.
[Register Rule] When a rule is registered in the rule screen.
[Modify Rule] When a rule is modified in the rule screen.
[Delete Rule] When a rule is deleted in the rule screen.
[Restore Rule] When a saved rule is restored in the rule screen*2.
[Initialize Rule] When a rule is initialized in the rule screen.
[Enable Rule Settings] When a setting for a rule is enabled.
[Disable Rule Settings] When a setting for a rule is disabled.

*1 A deletion is not recorded when an entry is automatically deleted because the maximum number of
registered entries was exceeded.
*2 A log is displayed for each rule type.

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● [Error Log] screen

[Date] Displays the date and time the log was registered.
[Logon Name] Displays the name of the user that was logged on when the log was registered.
[Status] Displays the status of an error that occurred.
[Occurred]: Error
[Clear]: Error cleared
[Error] Displays the message for the error that occurred.
[Error Code]* Displays the error code of the error that occurred.
[Parameter 1]*/ Displays parameter 1 and parameter 2 of the error that occurred.
[Parameter 2]* Depending on the type of error, this field may be blank.
[Comments]* Displays the entered comments.

* When the Details button is touched, the details appear below the log list.

● [Maintenance Log] screen

[Date] Displays the date and time the log was registered.
[Logon Name] Displays the name of the user that was logged on when the log was registered.
[Maintenance] Displays the name of the executed maintenance task.
[Maintenance Displays the properties of the executed maintenance task.
Property]*
[Comments]* Displays the entered comments.

* When the Details button is touched, the details appear below the log list.

A maintenance log is registered when one of the maintenance tasks below is performed.
The maintenance tasks and properties that are displayed are shown below.

Maintenance
Maintenance tasks
properties
[Auto Rinse] [As needed]
[Routine Cleaning] [Daily]
(Including routine cleaning at shutdown)
[Drain Waste Fluid Chamber] [As needed]
[Rinse Waste Fluid Chamber] [As needed]
[Remove Flowcell Air Bubbles] [As needed]
[Rinse Flowcell] [As needed]
[Drain Reaction Chamber] [As needed]
[Drain RBC Isolation Chamber] [As needed]
[Remove RBC Detector Clog] [As needed]
[Reagent Replenishment] [As needed]
[Reset Air Pump Count] [Part Replacement]

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 Chapter 4 Checking Logs

● [Reagent Replacement Log] screen

[Date] Displays the date and time the log was registered.
[Logon Name] Displays the name of the user that was logged on when the log was registered.
[Reagent] Displays the name of the replaced reagent.
[Lot No.] Displays the lot number of the replaced reagent.
[Exp. Date] Displays the expiration date of the replaced reagent.
[Serial No.]* Displays the serial number within the lot of the replaced reagent.
[Exp. date after Displays the shelf life of the replaced reagent after it has been opened.
opening]*
[Amounts]* If a diluent or a hemolytic agent was replaced, the amount of the replaced reagent
is displayed.
If a dye was replaced, the number of tests for the replaced reagent is displayed.
[Entry Type]* Displays the method of input used for the replaced reagent.
[Manual]: Manual
[Barcode]: Barcode reader
[RFID]: ID reader of the dye
[ProductCode]* Displays the entered product code.
[Manufacturer]* Displays the entered manufacturer.
[Address]* Displays the entered manufacturer address.
[Comments]* Displays the entered comments.

* When the Details button is touched, the details appear below the log list.

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 Chapter 5 IP Messages

Chapter 5 IP Messages

5.1 Overview of IP messages

When analysis data is analyzed, information that supplements the Positive/Negative sample judgment appears in the
[Data Browser] screen.
Results without an analysis error are classified as Positive or Negative based on preset criteria. The system judges
flags for analysis data based on comprehensive surveys of numerical data, distributions and scattergrams, and
provides easily-to-understand messages indicating the results. These messages are referred to as "IP (Interpretive
Program) messages".
IP messages appear in the flag display area in the following screen.
• Sample information tab in the [Sample Explorer] screen
• [Main] tab in the [Data Browser] screen
• [Graph] tab in the [Data Browser] screen

Caution!
• A Positive or Error judgment indicates the possibility of an abnormality. It is not a diagnosis of the patient.
If a Positive or Error judgment occurs, check the data and repeat the analysis, or examine carefully in
accordance with the protocol of your laboratory.
• IP messages are only intended for use in the clinical laboratory and are not for patient diagnosis. IP
messages provide notification of the possibility of a specific sample abnormality based on examination of
the analysis data.

Positive/Negative judgment

Flag display area

[Main] tab in the [Data Browser] screen

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 Chapter 5 IP Messages

● Message types
There are 2 types of IP messages that may be displayed for WBC, RBC/RET, and PLT: abnormal messages and
suspect messages.

Abnormal messages Indicates that the sample is clearly abnormal.

With some exceptions, the criteria for abnormal message judgment can be
preset.
Suspect messages Indicates a possibility that the sample is abnormal.

● Positive/Negative judgment

[Positive] Indicates that a blood cell analysis value or cell morphology exceeds the preset
criteria for the IP message (abnormal sample).
Displayed on a red background.
A Positive judgment is classified into the 3 types shown below. Touch [Positive]
to display a dialog box.
[Diff. Abnormal]: Indicates an abnormal blood cell differentiation value.
[Morph. Abnormal]: Indicates an abnormal cell morphology.
[Count Abnormal]: Indicates an abnormal blood cell count.
[Negative] Indicates that there were no analysis errors or abnormalities, and that there is no
IP message (normal sample).
Displayed on a green background.

Note:
Only Positive judgment is performed for analysis in [Pre-Dilution] mode and [Body Fluid] mode. Negative
judgment is not performed.

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 Chapter 5 IP Messages

With respect to the following IP messages, when a sample judgment is Positive, the analysis results are regarded as
having low reliability due to the abnormality, and " * " is displayed to the right of the data or "- - - -" which is a data
mask indicating non-analyzable is displayed.

WBC IP messages
WBC-BF
AS-LYMP#*2 TC-BF#
NEUT# LYMPH# MONO# EO# BASO# IG# AS-LYMP%*2 PMN#
WBC
NEUT% LYMPH% MONO% EO% BASO% IG% RE-LYMP#*2 PMN%
RE-LYMP%*2 MN#
MN%
WBC Abn Scattergram
Lymph, Mono   
Neut, Eo   
Lymph, Neut    
Neut, Mono   
Lymph, Baso 
Mono, Eo  
Mono, Baso  
Neut, Baso 
Debris, Neut        
Debris, Baso        
Debris, Lymph        
Debris, WBC-BF*1 
5 DIFF data
calculation ---- ---- ---- ---- ---- ---- ----
impossible
Abnormal IG

clustering
HF-BF high value
Left Shift?   
Blasts/Abn Lympho?     ----
Atypical Lympho?     
NRBC?        
*1 Does not appear when the instrument does not have the body fluid analysis function.
*2 The availability of analysis functions depends on your systems configuration.

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 Chapter 5 IP Messages

RBC/RET IP messages
RET#
RBC
RET%
RET#
HGB IRF
HCT
MCH RDW-SD RDW-CV LFR
MCV
MCHC MFR
MCH
HFR
MCHC
RET-He
RBC Abn Distribution
MP-Flag  ---- ----
Abnormal RDW-SD  ---- 
Other abnormal
  
distributions
Dimorphic Population ---- ----
*1
RET Abn Scattergram 
RBC Agglutination? 
Turbidity/HGB Interf? 
Iron Deficiency?
HGB Defect?
Fragment?
iRBC?*2
*1 Does not appear when the instrument does not have the RET analysis function.
*2 Only appears when the iRBC flag license is registered.

PLT IP messages
PDW
MPV
PLT
P-LCR
PCT
PLT Abn Distribution
Abnormal PDW ----
Other abnormal

distributions
PLT Abn Scattergram*
Foreign particles in PLT
area (serious effect)
Foreign particles in PLT
area
PLT Clumps?  
* Does not appear when the instrument does not have the RET analysis function.

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 Chapter 5 IP Messages

5.2 List of IP messages

Judgment value settings for IP messages can be changed. For details, see "Basic Operation".
(➤Basic Operation, "Chapter 7: 7.6 Data analysis settings")

● WBC IP messages
Messages Meaning Judgment method/equation
Abnormal messages
WBC Abn Scattergram Abnormal WBC scattergram Judged from clustering in the WDF scattergram.
For body fluid analysis, judged from clustering in
the WDF scattergram and the HF-BF value.
Neutropenia Low neutrophil count NEUT# < 1.00 x 103/μL
or NEUT% < 0.0%
Neutrophilia High neutrophil count NEUT# > 11.00 x 103/μL
or NEUT% > 100.0%
Lymphopenia Low lymphocyte count LYMPH# < 0.80 x 103/μL
or LYMPH% < 0.0%
Lymphocytosis High lymphocyte count LYMPH# > 4.00 x 103/μL
or LYMPH% > 100.0%
Monocytosis High monocyte count MONO# > 1.00 x 103/μL
or MONO% > 100.0%
Eosinophilia High eosinophil count EO# > 0.70 x 103/μL
or EO% > 100.0%
Basophilia High basophil count BASO# > 0.20 x 103/μL
or BASO% > 100.0%
Leukocytopenia Low leukocyte count WBC < 2.50 x 103/μL
Leukocytosis High leukocyte count WBC > 18.00 x 103/μL
IG Present Increased immature IG# > 0.10 x 103/μL
granulocyte or IG% > 100.0%
Suspect messages
Left Shift? Possibility of left shift Judged from the distribution of the upper right
area of NEUT in the WDF scattergram.
Blasts/Abn Lympho? Possibility that blasts are Judged from the presence of Blasts/Abn Lympho
present/Possibility of on the WDF scattergram.
abnormal lymphocytes
Atypical Lympho? Possibility of atypical Judged from the distribution of the upper area of
lymphocytes LYMPH in the WDF scattergram.
NRBC? Possibility of nucleated red Judged from the presence of NRBC in the WDF
blood cells scattergram.

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 Chapter 5 IP Messages

● RBC/RET IP messages
Messages Meaning Judgment method/equation
Abnormal messages
RBC Abn Distribution Abnormal RBC distribution Judged from the red blood cell distribution.
Dimorphic Population Double-peak RBC distribution Judged from the shape of the distribution peak
and the gap between the high and low points.
RET Abn Scattergram*1 Abnormal RET scattergram Judged from clustering in the RET scattergram.
Reticulocytosis*1 Reticulocytosis RET% > 5.0%
or RET# > 0.2000 x 106/μL
Anisocytosis Anisocytosis RDW-SD > 65.0 fL
or RDW-CV > 20.0%
Microcytosis Microcytosis MCV < 70.0 fL
Macrocytosis Macrocytosis MCV > 110.0 fL
Hypochromia Hypochromia MCHC < 29.0 g/dL
Anemia Anemia HGB < 10.0 g/dL
Erythrocytosis Erythrocytosis RBC > 6.50 x 106/μL
Suspect messages
RBC Agglutination? Possibility of RBC agglutination Judged from the red blood cell distribution and
hemoglobin related parameters.
Turbidity/HGB Interf? Possibility of effect on HGB Judged from the red blood cell distribution and
by chylemia hemoglobin related parameters.
Iron Deficiency? Possibility of iron deficiency Judged from the red blood cell distribution and
hemoglobin related parameters.
HGB Defect? Possibility of HGB Judged from the red blood cell distribution
abnormality related parameters.
Fragments? Possibility of fragmented red Judged from the red blood cell distribution,
blood cells platelet distribution, and RET scattergram.
iRBC?*2 Possibility of erythrocyte Judged from various analysis data and
inclusions scattergrams.
*1 Does not appear when the instrument does not have the RET analysis function.
*2 Only appears when the iRBC flag license is registered.

● PLT IP messages
Messages Meaning Judgment method/equation
Abnormal messages
PLT Abn Distribution Abnormal PLT distribution Judged from the platelet distribution.
Thrombocytopenia Thrombocytopenia PLT# < 60 x 103/μL
Thrombocytosis Thrombocytosis PLT# > 600 x 103/μL
PLT Abn Scattergram* Abnormal platelet Judged from clustering in the RET (PLT-O)
scattergram scattergram.
Suspect messages
PLT Clumps? Possibility of PLT clumps Judged from the presence of PLT clumps in the
WDF scattergram.
* Does not appear when the instrument does not have the RET analysis function.

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 Chapter 5 IP Messages

5.3 IP message judgment conditions and judgment methods

In the following cases, IP message judgment is not performed.
• QC analysis data
• Calibration analysis data
• Blank data
• Background check data
• Insufficient blood volume
• Adjustment

Blank data
Blank data is data that meets all of the following conditions:
• WBC < 1.00 x 103/μL
• RBC < 0.30 x 106/μL
• HGB < 1.0 g/dL
• PLT < 20 x 103/μL

● Judgment methods
WBC < 0.50 x 103/μL Judgment for a WBC suspect message ([Left Shift?]) is not performed.
(In [Pre-Dilution] mode, when WBC < 0.20 x 103/μL.)
RBC < 0.50 x 106/μL IP message judgment for RBC other than [RBC Abn Distribution] is not performed.
This is displayed as [RBC Abn Distribution], even if analysis of RBC was not
specified.

• If an error or other condition prevents an analysis parameter necessary for judgment from being calculated
("- - - -" or "+ + + +" is displayed), judgments including that parameter will not be performed.
• Parameters for which the user has not specified that analysis be performed (blank " ") are not used for
judgment.

● Analysis in [Pre-Dilution] mode or [Body Fluid]* mode

For IP messages judged in analysis in [Pre-Dilution] mode or [Body Fluid] mode, see the following.
(➤P.136 "● Table of IP message judgments")
Only Positive judgment is performed; Negative judgment is not performed.
* The availability of the body fluid analysis function depends on your system configuration.

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 BF [Body
Positive/Negative WB [Whole Blood]/LW [Low WBC] PD [Pre-Dilution]
Flag Detection Fluid]

*4
*3
*2
*1
Messages judgment flag
No.*1 channel CBC+DIFF CBC+DIFF

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category CBC CBC+DIFF CBC+RET CBC CBC+DIFF CBC+DIFF
+RET +RET
Chapter 5

WBC Abn Scattergram 1 Morph WDF ✓ ✓ ✓ ✓ ✓ ✓ ✓ ✓

Neutropenia 2 Diff WDF - ✓ - ✓ - ✓ ✓ -
Neutrophilia 3 Diff WDF - ✓ - ✓ - ✓ ✓ -
Lymphopenia 4 Diff WDF - ✓ - ✓ - ✓ ✓ -
Lymphocytosis 5 Diff WDF - ✓ - ✓ - ✓ ✓ -
Monocytosis 6 Diff WDF - ✓ - ✓ - ✓ ✓ -
IP Messages

Eosinophilia 7 Diff WDF - ✓ - ✓ - ✓ ✓ -

Basophilia 8 Diff WDF - ✓ - ✓ - ✓ ✓ -

WBC
Abnormal messages
Leukocytopenia 9 Count WDF ✓ ✓ ✓ ✓ ✓ ✓ ✓ -
Leukocytosis A Count WDF ✓ ✓ ✓ ✓ ✓ ✓ ✓ -
IG Present F Morph+Count WDF - ✓ - ✓ - ✓ ✓ -
Left Shift? 3 Morph WDF - ✓ - ✓ - ✓ ✓ -
Blasts/Abn Lympho? 7 Morph WDF - ✓ - ✓ - ✓ ✓ -
Atypical Lympho? 4 Morph WDF - ✓ - ✓ - ✓ ✓ -

Suspect
messages
NRBC? 5 Morph WDF - ✓ - ✓ - ✓ ✓ -
RBC Abn Distribution 1 Morph RBC ✓ ✓ ✓ ✓ - - - -
Dimorphic Population 2 Morph RBC ✓ ✓ ✓ ✓ - - - -
● Table of IP message judgments

Judgements are dependent on the utilized channels.

RET Abn Scattergram*2 9 Count RET - - ✓ ✓ - - - -

Only appears when the iRBC flag license is registered.

Reticulocytosis*2 A Count RET - - ✓ ✓ - - ✓ -
Anisocytosis 3 Morph RBC ✓ ✓ ✓ ✓ ✓ ✓ ✓ -
Microcytosis 4 Morph RBC ✓ ✓ ✓ ✓ ✓ ✓ ✓ -
Macrocytosis 5 Morph RBC ✓ ✓ ✓ ✓ ✓ ✓ ✓ -

Abnormal messages
Hypochromia 6 Morph RBC+HGB ✓ ✓ ✓ ✓ ✓ ✓ ✓ -
Anemia 7 Count HGB ✓ ✓ ✓ ✓ ✓ ✓ ✓ -

RBC/RET
In the [Sample Explorer] screen, IP messages are shown by flag number.
Erythrocytosis 8 Count RBC ✓ ✓ ✓ ✓ ✓ ✓ ✓ -
RBC Agglutination? 1 Count RBC+HGB ✓ ✓ ✓ ✓ - - - -
Turbidity/HGB Interf? 2 Count RBC+HGB - - - -

Does not appear when the instrument does not have the RET analysis function.
✓ ✓ ✓ ✓
Iron Deficiency? 3 Morph RBC+HGB ✓ ✓ ✓ ✓ - - - -
HGB Defect? 4 Morph RBC ✓ ✓ ✓ ✓ - - - -
Fragments? 5 Morph RBC, PLT, RET ✓ ✓ ✓ ✓ - - - -

Suspect messages
iRBC?*3 6 Morph WDF, PLT, RET ✓*4 ✓*4 ✓*4 ✓ ✓*4 ✓*4 ✓ -
PLT Abn Distribution 1 Morph PLT ✓ ✓ ✓ ✓ - - - -
Thrombocytopenia 2 Count PLT, RET ✓ ✓ ✓ ✓ ✓ ✓ ✓ -
Thrombocytosis 3 Count PLT, RET ✓ ✓ ✓ ✓ ✓ ✓ ✓ -

Abnormal
messages
PLT Abn Scattergram*2 4 Count RET - - ✓ ✓ - - - -

PLT
PLT Clumps? 1 Count WDF ✓ ✓ ✓ ✓ - - - -

Suspect
messages

XN-L series XN-550/XN-450/XN-350 Troubleshooting

 Index

Index

A E
Auto rinse..............................................27, 33, 47 Error messages................................................... 7

C H
Calibration........................................................101 [Help] dialog box ............................................... 11
Main functions .............................................102
Overview......................................................101 I
Standards ....................................................101 IP messages
Calibration logs ................................................113 Details table ................................................ 136
[Calibration History] screen .........................117 Displayed items .......................................... 129
Backup.........................................................115 Judgment conditions................................... 135
Check ..........................................................113 Judgment methods ..................................... 135
Delete ..........................................................116 Meaning ...................................................... 133
Output..........................................................113 Types .......................................................... 133
Output in CSV format ..................................114
Restore ........................................................115 L
Calibrator .........................................................102 List of maintenance tasks.................................. 69
Calibrator calibration ........................................103 Logs ................................................................ 121
Calibrators and samples to be used ............102 [Audit Log] screen....................................... 124
Overview......................................................101 [Error Log] screen ....................................... 126
Screens .......................................................117 [Maintenance Log] screen .......................... 126
Causes of errors and remedial actions ..............12 [Reagent Replacement Log] screen ........... 127
Errors related to the analysis.........................33 Check.......................................................... 121
Errors related to the blood aspiration ............26 Comments .................................................. 121
Errors related to the covers ...........................50 Output ......................................................... 122
Errors related to the dye holder .....................60 Output in CSV format.................................. 122
Errors related to the hand and sample tube
holders..............................................67 M
Errors related to the laser ..............................31 Maintenance and inspection checklist............. 100
Errors related to the motors...........................25 Maintenance screens ........................................ 70
Errors related to the orders............................30 [Exchange] dialog box .................................. 72
Errors related to the pressure........................12 [Maintenance] menu screen ......................... 71
Errors related to the quality control (QC).......32 [Operation Confirmation] dialog box ............. 71
Errors related to the reagents and [Rinse Instrument] dialog box ....................... 72
chambers..........................................16 [Status Confirmation] dialog box................... 73
Errors related to the sample numbers ...........30 Monitor
Errors related to the sample tube holder .......30 Changing settings ......................................... 98
Errors related to the sampler analysis Closing the OSD screen ............................... 98
operation...........................................66 Displaying the OSD screen........................... 98
Errors related to the system ..........................31 Image quality adjustment.............................. 98
Errors related to the temperature ..................15 Setting items in the OSD screen................... 99
Errors related to the user maintenance and
warnings ...........................................51 O
Cleaning the aspiration unit tray (XN-350).........96 Operation tests.................................................. 79
Cleaning the aspiration unit tray Air pump ....................................................... 79
(XN-550/XN-450) ............................................94 Aspiration unit motor..................................... 79
Counter ..............................................................82 Barcode reader (XN-550) ............................. 80
Motor............................................................. 79
D Pinch valve ................................................... 80
Draining the pneumatic trap chamber................13 Sampler ........................................................ 81
Draining the sample from the RBC isolation Sheath motor ................................................ 79
chamber ..........................................................34 Whole blood aspiration motor ....................... 79

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 Index

P
Precision check ............................................... 109
Calibrators and samples to be used ........... 102
Overview..................................................... 101
Screens....................................................... 119
Precision check logs ....................................... 113
[Precision Check History] screen................ 119
Backup........................................................ 115
Check.......................................................... 113
Delete ......................................................... 116
Output ......................................................... 113
Output in CSV format.................................. 114
Restore ....................................................... 115

R
Reagent replacement........................................ 84
Dialog box..................................................... 84
Diluent and hemolytic agent .............16, 57, 85
Dye ............................................ 17, 58, 61, 86
Reagent replenishment ..................................... 77
Registering reagent information ........................ 63
Removing air bubbles from the flowcell ............ 46
Removing the RBC detector clog...................... 34
Replacing the air pump ............................... 54, 89
Replacing the fuse ............................................ 92
Replacing the piercer
(XN-550/XN-450) ........................................... 88
Replacing the waste container .................... 23, 78
Rinsing the flowcell (XN-350)...................... 39, 45
Rinsing the flowcell (XN-450)...................... 38, 44
Rinsing the flowcell (XN-550)...................... 37, 43
Rinsing the RBC detector aperture ............. 35, 40
Rinsing the waste chamber (XN-350) ............... 22
Rinsing the waste chamber (XN-450) ............... 21
Rinsing the waste chamber (XN-550) ............... 20
Routine cleaning (XN-350)............. 29, 49, 53, 76
Routine cleaning (XN-450)............. 28, 48, 52, 75
Routine cleaning (XN-550)............. 27, 47, 51, 74

S
Sensor............................................................... 83

X
XN CAL ........................................................... 102

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