12.
BIOTECHNOLOGY
MULTIPLE CHOICE QUESTIONS ( 1 MARK EACH)
1 The technique which involves addition or deletion of genes is….
A genetic engineering
B gene therapy
C gene splicing
D gene piracy
2 ECoRI is obtained from…
A Escherichia coli R13
B Escherichia coli Ry13
E
C Escherichia coli R225
D Escherichia coli RC
N
3 The enzyme restriction endonuclease …
A cuts double strand of DNA
B joins strand of DNA
C cuts RNA strand
4
D cuts single stranded DNA U
Ti plasmid being used for introducing genes in plants obtained from….
A Agrobacterium rhizogenes
,P
B Escherichia coli
C Agrobacterium T20
D Agrobacterium tumefaciens
TM
Polymerase chain reaction is most useful in….
A DNA amplification
B DNA synthesis
C protein synthesis
D selective replication of DNA
5 In Bt cotton a transgenic plant, Bt refers to….
ER
A bold cotton
B Bacillus thuringiensis
C beta carotene
D tumor inducing bacteria
7 In transgenic crop substance provitamin A is obtained in….
SC
A rice
B tomato
C canola
D sugarcane
8 In Anaemia the Recombinant protein….is produced by r-DNA technology.
A Relasein
B Insulin
C Erythroprotein
D Antoitrpsin
9 In biotechnology GMO refers to….
A generation mediated organisms
B genetically modified organisms
C good modified organisms
D gross modified organisms
10 First biopatent to genetically engineered bacterium….
A Pseudomonas
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B Agrobacterium
C Azatobacter
D E. coli.
VERY SHORT ANSWER TYPE QUESTIONS ( 1 MARK EACH )
1 In which transgenic plant the substance Flavonoids obtained as antioxidants.
2 What is Germline therapy?
3 Which Recombinant proteins is obtained for Hepatitis-B by r-DNA technology.
4 What is plasmid?
5 What is Palindromic sequence?
6 Alu-I is obtained from which organism?
7 What is the role of Taq-polymerase in PCR technology?
8 Bt-cotton shows adverse effect on the population of which butterfly?
E
SHORT ANSWER TYPE QUESTIONS(SA-I) (2 MARKS)
N
1 What is Biopiracy? Explain it with respect to Turmeric.
2 How Biotechnology is applicable with respect to Genomics?
3 Explain how transgenic fish is commercially beneficial.
4
5
produced?
U
Write any two human disorders and to cure which recombinant proteins are
For production of edible vaccines plants are used. Explain this any one
,P
example.
6 Write a note on uses of somatic cell gene therapy.
7 Define vector? write any two examples.
TM
SHORT ANSWER TYPE QUESTIONS(SA-II) (3 MARKS)
1 Explain traditional use of Biotechnology.
2 Define biotechnology? Which are the basic principles and process of
biotechnology?
ER
3 What is gene cloning? Explain different tools used for it.
4 Explain types of enzymes used in biotechnology?
5 What is Recognition sequence? Explain in brief.
6 Define Biotechnology? How it is used in production of Human insulin.
7 What is GM plant? Write its different advantages.
SC
LONG ANSWER TYPE QUESTIONS (LA) (4 MARKS)
1 What is PCR? Explain different steps involved in it.
2 Explain the following terms with respect to rDNA technology
i) passanger DNA
ii) Chimeric DNA
iii) Transformed cell
iv) restriction site
3 Define biotechnology. Give any three application of it?
4 Which are different adverse effect of biotechnology on human health and
environment?
5 Explain biopatent and Biopiracy with different examples?
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