Thakur College of Science & Commerce (Autonomous) Library Question papers T.Y.B.Sc — BT Sem—V (R) (October 2023) (2023-24)THOT TY | Molecular biolegy 18) 10]22 Code IEXBTSM B32 y_ ‘Time : 2.00 hrs Max Marks : 60 Instructions: 9) All the questions are compulsory and carry equal marks. 10) Draw neat and labeled diagrams wherever necessary. 11) For Ql, (2, Q3 and Q4 attempt either A or B and Cor D. 12) Figures to the right indicate maximum marks. QUA. Justify: Ti plasmid is a large plasmid having a number of important components. oM oR QIB. Justify: Process of agrobacterium based gene transfer requires a number of co-ordinated 6M. steps. QUC. © What is microprojectile bombardment? Discuss its advantages and limitations’as a ™ Oo method for gene transfer. oR QUD. —_ What types of cells are formed after protoplast fusion? How can we select cells of o™M interest from the common pool of cells? QA. How is :DNA technology used for improving aquaculture? oM OR Q2B. What is molecular pharming? Explain with an example, 6M QC, Discuss PCR method for selection of engineered embryonic stem cells. o™ OR CHD. _ Discuss development ofa transgenic mice as a model for Alzheimer’s disease. oM QBA. _ Elaborate on the key features of yeast artificial chromosomes, oM OR QB. Elaborate on different major types of ‘vectors used in recombinant DNA technology. 6M GC. Mustrate the methodology to create chromosomal library. OM OR Q8D. Compare and contrast between the chromosome walking and chromosome jumping ™M strategy.Q4B. Q4c. Q5. eS rXaAWwALN Explain Automated DNA sequencing method and its advantages. OR Explain the classical chain termination method for DNA sequencing. Discuss limitations and advantages of different types of targeted nucleases. OR Explain the technology used for gene silencing, Attempt any four of the following. . Give use of PEG, CaClp and enzyme mixture in process of protoplast formation and fasion. ‘What are the voltages used for electroporation? }. Give limitations of microinjection method of gene transfer. ‘What is Cre recombinase? Give its use. . Elaborate on the method and application of homopolymer tailing’. Explain what is “mmunoscreening’ /. Elaborate on the significance of new generation sequencing. , Discuss major applications of CRISPR. om 6M 6M 12MTH eT jv | Mechical Bictechudogy Code: FXO TSM 3, Tipe: 2:00 Hrs Max Marks: 60 Roll No. Instructions: OLL1L, 1) Figures tothe right indicate full marks. All questions are compulsory and carry equal mats. 2) Draw neat and labeled diagrmas wherever necessary, 3) For Q1, Q2 ,Q3 and Q4 a tempt any one question from A and B and any one question from C and D. Q.1A_ Explain the different types of structures of viruses 6 OR Q. 1B Explain the one step growth curve experiment on bacteriophage. 6 Q.1C Discuss the adsoption, entry & synthesis of proteins of T4 phage infecting E.coli 6 OR Q. 1D _ Discuss the replication of Influenza virus 6 Q.2A Blaborate on any one cell wall synthesis inhibitor w.r.t types, mode of action, ADME. 6 properties and chemical uses, oR Q.2B_ Blaborate on tetracyclines as chemotherapeutic agents. 6 Q.2C Which antifungal agents are used for systemic mycoses? Discuss any three. 6 OR Q.2D What are the types of drugs used for treatment of HIV? 6 Q.3A Justify: Enzymes can be used as therapeutic agents. (Elaborate on any two) 6 OR Q.3B Justify: The isolation of interferons in the 1980s was a long and tedious process. 6 Q.3C__ What are chimeric monoclonal antibodies? Explain their structure, properties and 6 drawbacks, OR Q.3D What are the types and limitations of traditional vaccines? 6 Q.4A Describe any one technique used for assay of anti-microbial activity. 6 OR Q.4B Discuss the types of toxicity studies carried out during drug development. 6 Q.4C Elaborate on the differences in preclinical and clinical phases of drug development. 6 oR Q.4D Compare and contrast between Phase II and Phase Ill clinical trials, 6Q5. A) B) °) D) E) Fr) 9) H) Attempt any FOUR of the following, Illustrate the mechanism of choice between Lytic and Lysogenic Cycles Illustrate the Baltimore system of virus classification Enlist any three mechanisms of resistance. Describe mode of action and mechanism of resistance of chloramphenicol. Give significance of glycosidases in context of blood grouping. ‘What are humanized monoclonal antibodies? Highlight the importance of Phase IV clinical studies. Comment on the GCP guidelines for clinical trials. 12IOxBT SC BOTOI2> ee TOT [Be 16 (0 [2% ‘Time: 2hrs ‘Max marks: 60 Instructions: 1, All questions are compulsory and carry equal marks, 2. For Qt, Q2, Q3 & Q4 attempt either A or and C or D. 3. Figures on right indicate maximum marks. 4, Draw neat & Iabeled diagrams wherever necessary. Qi Qu Qu Qu oe Q2 Q2 Q2 Q3 Q4 Q4 Qs A wrue a wr bow Poo ° eI AUR Illustrate the relationship of cyclins and MPF levels during cell cycle. OR Discuss cyclin-cdk combinations in mammalian cell cycle. Ilustrate the extrinsic pathway for apoptosis. Elaborate on the andr of vents in programmed cell death and its biological importance. Describe any one signaling pathway initiated through RTKs. Desoribe the atvation and cellular response given by PKB. ‘What is the role of PKA in cell signaling? Explain with any one example. OR What is the role of IP3? Elaborate on the pathway that leads to its formation. What are germ layers? Describe the process that leads to their formation. How can fate hit be carried out using chimeric organisms? How is autonomous specification different from conditional specification? How do snorphogene gradients influence embryonic development in Drosophila? Justify, Meta, felon cancer is a multistep process.” Justify, “Activation of an oncogene in different ways can transform a normal cell to malignant state.’ Elaborate on the genetic basis for breast cancer. OR Elaborate on the characteristics of cancer celis. Answer any FOUR of the following Elaborate on the role of edks. Discuss the role of cytochrome C. What are the types of intercellular signaling? Give the role of GRK and arrestin. Enlist the stages of embryonic development. Define any two. Comment on Drosophila melanogaster as a model organism for studying development. Comment on Knudson Two-hit model. Explain the term “Guardian of genome’ and its relevance.Code: LOXAT 541505) 7 Time: ‘Max marks: Instructions: All questions carry equal marks and are compulsory. Figures on right indicate maximum marks. Qa Qa Qu Ow Q2 Q3 A Elaborate on biosafety levels, OR Elaborate on biological risk assessment. Justify. “A risk assessment will determine the degree of correlation between an agents risk group classification”. oR Elaborate on predominant probable routes of transmission. Discuss GLP principles involved in microbiology lab layout, equipment’s & maintenance of cultures OR Discuss documentation as per GLP Elaborate on SOP writing as per GLP OR Elaborate on steps involved in media preparation and storage as per GLP Explain the factors affecting microbial spoilage of pharmaceutical products oR Explain the effects of microbial attack on pharmaceutical products Summarize the various ways in which pharmaceutical products can get contaminated 6Q3 Q4 a4 Q4 Q4 Qs oR ‘Summarize various food bome pathogens & food borne illness Elaborate on the role of Chymosin, OR Elaborate on controversy about labeling genetically modified foods, Who benefits from molecular biotechnology? OR Elaborate on the impacts of genetically modified organisms on environment. Attempt any 4 from following Explain the basic pathway of risk assessment. Write a note on regulating rDNA technology. Demonstrate role of calibration & validation Ilustrate objectives of GLP Ilustrate the methods of control of contamination of pharmaceutical products ‘Demonstrate various quality control procedures of pharmaceutical products Explain biosafety level 1 and 4 ‘Write a note on containment,enh Ty $7 7-4 code: TRBT 5 MBJOY LL, — Timer dhrs ‘Max marks: 60 Instructions: Qi Qi Qu Qi Q2 Q2 7 (2 Qs Q3 Q3 GB Q4 Q4 Q4 Q4 Qs All questions are compulsory and carry equal marks, Figures on right indicate maximum marks, Draw neat & Iabeled diagrams wherever necessary, A. Describe the coral reef community OR B_ Describe the neretic community CC. Explain the culture dependent dilution to extinction assay & microdroplet encapsulation methods of bioprospecting OR D_ Explain the gene targeted approach of bioprospecting A. Discuss importance of various marine polysaccharides as fimetional food ingredients. OR Discuss importance of marine fungi, fish and fish waste nutraceutical purpose. Give an overview of approved drugs developed from marine source. OR Discuss the process of Hypothesis testing in case of t test OR B c D_ Give an account of marine anti-cancer agents. A B_ Discuss the process of Hypothesis testing in case of chi-square test for association C Solve: Out of 180 children examined for hearing disability, 20 were found to have some type of hearing abnormality. Does it confirm with the statement that 20% of school children have hearing abnormality? The population is normally distributed, OR D Solve: the weight of 10 rats (gms) when brought to lab & after 1 month were recorded & the following difference in weight was obtained- 4 4342 3, -1 21,5 Can it be concluded that there is no significant difference in weight when the guinea pigs are lab fed. (t 9.05, 9d-f. = 1.833) Explain the applications of IR spectroscopy and AAS R A or B_ Explain the advantages é limitations of liquid scintillation counter C_ Explain the solid scintillation radioactivity detectors OR D_ Explain the principle of IR spectroscopy Answer any FOUR of the following Illustrate the method of bioprospecting using isotope labeled substrateauAUN oN Demonstrate the type of life exists in deep sea zone Comment on marine active ingredients with potential use in skin care cosmetics. Discuss muttaceutical importance of lipids obtained from maarine origin. Illustrate the type I & type Ul errors Solve: Average height of 49 gitls, 19yr old, is 140 cm & standard deviation is 7. Find 95% confidence limit within which height of all 18 yr old girls would lie. Illustrate the construction of fluorescence spectroscopy Demonstrate applications of fluorescence spectroscopy ~ FRET, BRET & FRAP.