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                Biological Molecules
                   Question Paper 4
Level                            IGCSE
Subject                          Biology
Exam Board                       CIE
Topic                            Biological Molecules
Sub-Topic
Paper Type                       Alternative to Practical
Booklet                          Question Paper 4
Time Allowed:       56 minutes
Score:              /46
Percentage:         /100
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1   Fig. 2.1 shows an insect-pollinated flower, cut in half longitudinally.
                                                 Fig. 2.1
    (a) (i) Make a large drawing of the cut surface of the half-flower shown in Fig. 2.1.
                                                                                                 [4]
        (ii) On your drawing, label each of the following with a label line and the letter X, Y or Z:
             X for the part of the flower in which the pollen grains are produced,
             Y for the part of the flower to where the pollen grains are transferred during
             pollination,
             Z for the part of the flower through which the pollen tube grows, shortly after
             pollination.
                                                                                         [3]
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(b) (i) Insects such as the honey bee, Apis mellifera, collect nectar to make into honey.
        Describe how you could test a sample of honey for the presence of each of the
        following:
        reducing sugar;
        starch.
                                                                                          [3]
   (ii) Honey contains reducing sugar.
        State the colour change you would observe during the reducing sugar test in (b) (i).
                                                                                           [1]
(c) Fig. 2.2 shows one pollen grain, as seen with the aid of an electron microscope. This
    pollen grain has been magnified 200 times.
                                            Fig. 2.2
    (i) Calculate the actual size of this grain.
        Show your working.
                                                       actual size                         [2]
   (ii) State one feature visible in Fig. 2.2, that suggests that this pollen grain is from an
        insect-pollinated flower.
                                                                                          [1]
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(d) It has been suggested that petal colour is important to attract insects to collect nectar
    and to pollinate the flowers.
    (i) Outline how you would carry out an investigation to find out which petal colour
        would attract most insects.
                                                                                          [4]
   (ii) Some insect-pollinated flowers do not have brightly coloured petals to attract
        insects to collect nectar.
        Suggest how insects might be attracted to these flowers.
                                                                                          [1]
                                                                                   [Total 19]
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2   Seeds from the plant family Papilionaceae form an important part of the human diet.
    Fig. 1.1 shows three different types of seed that have been soaked in water for 24 hours.
        lentils                            chickpeas                      soya beans
                                              Fig. 1.1
    (a) Describe the differences in shape and appearance of the seed coat (testa) between
        the three types of seed.
        Write your answers in Table 1.1.
                                              Table 1.1
             feature                 lentil               chickpea              soya bean
     shape of seed
     appearance of
     seed coat
                                                                                                [3]
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(b) A group of students were planning an investigation into the effect of temperature on the
    germination of seeds.
    The teacher gave them a list of possible variables.
                 temperature            number of seeds germinated
                      intensity of light        time
                   length of seedling               volume of water
    From this list, select the most suitable:
                              variable to change;
                            variable to measure.
                                                                                         [2]
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    Fig. 1.2 shows the same three seeds after they have been germinated in suitable
    conditions.
           lentil                          chickpea                  soya bean
                                            Fig. 1.2
    (c) (i) Make a large, labelled drawing of the lentil seedling.
                                                                                 [4]
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    (ii) You are going to calculate the magnification of your drawing.
         Measure the length of the line ST on Fig. 1.2.
         length of line ST                                                           mm
         Draw line ST on your drawing in the same position as in Fig. 1.2.
         Measure the corresponding length of ST on your drawing.
         length of ST in drawing                                                     mm
         Calculate the magnification of your drawing.
         Show your working.
                                              magnification ×                        [4]
Lentils contain protein and a small quantity of fat.
(d) Describe the food tests you could carry out to show that lentil seeds contain:
    (i) protein;
                                                                                     [2]
    (ii) fat.
                                                                                     [3]
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(e) The percentage of protein and fat in five types of seed, are shown in Table 1.2.
                                       Table 1.2
         type of seed             percentage of protein / %          percentage of fat / %
           chickpea                           8.0                             2.5
             lentil                           9.0                             0.6
          lima bean                           8.0                             0.4
         mung bean                            7.0                             0.4
          soya bean                          16.0                             8.0
    (i) Construct a bar chart to show the percentages of protein and fat in the five types of
        seed. Use the same axes for the two sets of data.
                                                                                          [5]
   (ii) Meat is a good source of protein.
        Name the type of seed in Table 1.2 that would be a good alternative to meat in the
        human diet.
                                                                                             [1]
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Fig. 1.3 shows part of a label from a packet of soya bean seeds. The label shows the
energy content measured in kilojoules.
                                 Soya Beans
                          Nutrition
                          Typical            50 g serving
                          composition         provides
                          Energy                 230 kJ
                          Protein                 8.5 g
                          Carbohydrate            4.5 g
                          Fat                     4.0 g
                                      Fig. 1.3
Fig. 1.4 shows a simple calorimeter.
This apparatus can be used to find the energy content of a soya bean seed.
The soya bean seed is burned and the energy released is absorbed by the water in the
test-tube.
                               thermometer
                       water
                    burning soya
                    bean seed
                                      Fig. 1.4
(f) Suggest how you could safely carry out a simple investigation to find the energy
    content of a sample of soya bean seeds.
   State what you would need to measure and control.
                                                                                  [3]
                                                                          [Total: 27]