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Exp 5 - Effect of Saline Purgatives

The experiment aims to study the effect of saline purgatives on frog intestine by using different solutions in isolated compartments. Hypotonic solutions cause fluid to move into circulation, shrinking the intestine, while hypertonic solutions swell the intestine by drawing fluid into the lumen. The isotonic solution shows no movement across the intestinal membrane, confirming the purgative effect of the hypertonic solution.

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156 views1 page

Exp 5 - Effect of Saline Purgatives

The experiment aims to study the effect of saline purgatives on frog intestine by using different solutions in isolated compartments. Hypotonic solutions cause fluid to move into circulation, shrinking the intestine, while hypertonic solutions swell the intestine by drawing fluid into the lumen. The isotonic solution shows no movement across the intestinal membrane, confirming the purgative effect of the hypertonic solution.

Uploaded by

kushaldubey48053
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Accurate College of Pharmacy

B. Pharm 3rd Year Pharmacology-III Practical


Experiment – 05

Aim: To study the effect of saline purgatives on frog intestine.

Reference: Ex-Pharm series software.

Theory:

Saline purgatives are the salts comprising of highly charged ions and do not cross cell
membrane freely. They remain inside the lumen and retain water through osmotic forces. They increase
the volume of the contents of the bowel, stretch the colon and produces normal stimulus for contraction
of the muscle that leads to defecation. Saline purgatives are primarily used in the treatment of
constipation.

Procedure:

1. Pith the frog and place it on a dissecting board.


2. Expose the abdominal cavity and carefully trace the small intestine.
3. Make the small intestine into three compartments by tying threads of different colours in such
a way that no fluid can move from one compartment to the other.
4. Inject 0.2 ml of each hypotonic solution (Normal Saline) into first compartment, 0.2 ml of
hypertonic solution (27% MgSO4 solution) to second compartment and 0.2 ml of isotonic
solution (Frog Ringer Solution) into third compartment.
5. Wait for 20 minutes and the observations are to be recorded.

OBSERVATION:

Before injection of Solutions 20 minutes after injection of solutions

Observation description: Hypotonic solution causes the fluid to move from lumen into circulation by
process osmosis thereby shrinks the intestine. Hypertonic solution (saline purgative) moves the fluid
from cells into the lumen and swells the intestine and isotonic solution did not show any fluid movement
across the intestinal membrane.

Report: Hypertonic solution (saline purgative) moves the fluid from cells into the lumen and swells the
intestine and shows purgative effect.

Mayank Sharma (Assistant Professor)


Dept. of Pharmacology

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