The Implications of Sports Biomechanics Studies On The Research and Development of Running Shoes: A Systematic Review
The Implications of Sports Biomechanics Studies On The Research and Development of Running Shoes: A Systematic Review
Systematic Review
The Implications of Sports Biomechanics Studies on the Research
and Development of Running Shoes: A Systematic Review
Shuangshuang Lin 1 , Yang Song 1,2 , Xuanzhen Cen 1,2 , Kovács Bálint 1 , Gusztáv Fekete 3 and Dong Sun 1, *
Abstract: Although various sports footwear demonstrated marked changes in running biomechanical
variables, few studies have yielded definitive findings on the underlying mechanisms of shoe
constructions affecting running-related performance and injuries. Therefore, this study focused
on examining the effect of basic shoe constructions on running biomechanics and assessing the
current state of sports shoe production in terms of injury and efficiency. Relevant literature was
searched on five databases using Boolean logic operation and then screened by eligibility criteria. A
total of 1260 related articles were retrieved in this review, and 41 articles that met the requirements
were finally included, mainly covering the influence of midsole, longitudinal bending stiffness,
heel-toe drop, shoe mass, heel flare, and heel stabilizer on running-related performance and injuries.
The results of this review study were: (1) The functional positioning of running shoe design and
the target groups tend to influence running performance and injury risk; (2) Thickness of 15–20 mm,
hardness of Asker C50-C55 of the midsole, the design of the medial or lateral heel flares of 15◦ , the
curved carbon plate, and the 3D printed heel cup may be beneficial to optimize performance and
Citation: Lin, S.; Song, Y.; Cen, X.;
reduce running-related injuries; (3) The update of research and development concepts in sports
Bálint, K.; Fekete, G.; Sun, D. The
biomechanics may further contribute to the development of running shoes; (4) Footwear design and
Implications of Sports Biomechanics
optimization should also consider the influences of runners’ strike patterns.
Studies on the Research and
Development of Running Shoes: A
Systematic Review. Bioengineering
Keywords: running shoes; biomechanics; performance; injuries; development
2022, 9, 497. https://doi.org/
10.3390/bioengineering9100497
ineffective in reducing runners’ lower limb and foot injuries. In addition, among the most
striking patterns arising from studies of sports shoe biomechanics, many results are the
indirect product of shoe-induced kinematic changes. Since a comprehensive upgrade in
athletic footwear is warranted, recognizing biomechanical adaptations aligned with shoe
technologies is vital to understanding the mechanism of any potential outcomes.
As one of the most notable footwear elements, the sole has undergone massive adjust-
ments (e.g., sole geometry and properties). In the previous articles, the role of sole shoe
characteristics on running biomechanics was discussed. Chambon [8] stated that increased
midsole thickness had no effect on foot-strike patterns or kinematics but influenced stance
phase duration. A broad range of heel-to-toe drops applied in running shoes has been
found to stimulate distinct foot-strike patterns and injury potentials among different run-
ning crowds (i.e., regular, occasional, or untrained) [9]. Moreover, Willwacher et al. [10]
deduced that variable stiffness shoes could increase anterior ground reaction force, which
supports athletes’ forward propulsion and potentially improves running efficiency. By
offloading foot joints during locomotion, an enhanced forefoot bending stiffness can serve
a preventative role in running injuries [11]. On the other hand, Law et al. [12] concluded
that individuals might respond inconsistently to the modification of sole stiffness owing to
the disparity of gait and muscle activation patterns.
Although there are also various sports shoe constructions that have demonstrated
marked changes in running biomechanical variables, such as shoe mass, heel flare, and heel
stabilizer [1,4], few studies have yielded definitive findings on the mechanisms underlying
shoe features affecting running-related performance and injuries. To underpin modern
sports shoe designs, this study focused on examining the effect of differing sole features on
running biomechanics and assessing the current state of the production of sports shoes in
terms of injury and efficiency.
Figure
Figure PRISMA
1. 1. Flow
PRISMA Chart
Flow for Systematic
Chart review.
for Systematic review.
2.4.Eligibility
2.3. Data Extraction
Criteria and Management
Eligibility criteriain
All the articles ofthis
the study
literature
were in downloaded
this study: (1)by Original
the sameresearch
authorfrom
and peer-re-
imported into
viewed
MendeleyEnglish journalsManagement
Reference was included. Conference
Software papers,
(Elsevier review
Ltd., papers, master
Amsterdam, (doc-
The Netherlands)
toral) graduation
for duplicate papers, casearticles
elimination, studies, and non-full and
management, text articles
citation.were
Theexcluded.
other two(2) The con-
authors
research
ductedmust
data be related toand
extraction the midsole
analysis(hardness, thickness,
of the literature, and material),
mainly including bending stiff-
the first author’s
ness,
name,heel-to-toe
researchdrop, shoe mass,
publication heelcountry,
year, flare, andresearch
heel stabilizer
design,(heel cup, heel
athletic counter) of
performance-related
running
and/orshoes. (3) The research
injuries-related must involve
biomechanics the corresponding
variables, and the primarystatistical analysis
results. and
Disagreements
offer quantitative
regarding data results on the
extraction influences
were resolved of by
shoe construction
the correspondingin biomechanical changes The
authors if happened.
during
PRISMA running that are
checklist was associated
followedwith performance
to synthesize and/or running injuries; non-biome-
data.
chanical related studies were excluded.
2.5. Quality Assessment
2.4. Data Extraction and Management
Cochrane Risk of Bias Assessment Tool was used to assess the risk of bias in each study
All the
by two articlesDisagreements
authors. in this study were downloaded
of quality by the same
assessment were author andbyimported
resolved into
the corresponding
Mendeley Reference Management Software (Elsevier Ltd., Amsterdam, The Netherlands)
authors if happened. Seven domains were evaluated (random sequence generation, alloca-
for duplicate
tion elimination,
concealment, articles
blinding management,
of participants andand citation. The
personnel, other of
blinding two authorsassessment,
outcome con-
ducted data extraction and analysis of the literature, mainly including the first author’s
incomplete outcome data, selective reporting, and other biases) and each domain has three
name, research publication year, country, research design, athletic performance-related
grades, i.e., low risk of bias, unclear risk of bias, and high risk of bias.
and/or injuries-related biomechanics variables, and the primary results. Disagreements
regarding data extraction were resolved by the corresponding authors if happened. The
3. Results
PRISMA checklist was followed to synthesize data.
3.1. Basic Characteristics of Included Studies
The comprehensive research generated 1230 articles (as shown in Figure 1). After
removing duplicated articles, a total of 596 articles was included. After two authors
Bioengineering 2022, 9, 497 4 of 29
conducted a title and abstract screening, a total of 125 articles was included. The articles
were further screened through the three eligibility criteria, and 41 related articles were
finally used in this study.
According to the authors’ country/region, 17 studies were from Europe, including
4 from France, 3 from Germany, 3 from Luxembourg, 2 from the United Kingdom, 2
from Austria, 1 from Belgium, 1 from Spain, and 1 from Switzerland; a total of 19 studies
were from North America, including 10 from Canada and 9 from the United States, and
6 from Asia, including 5 from China and 1 from South Korea. The proportion of research
literature published in 1980–1999, 2000–2009, and 2010–2021 was 11.90%, 23.81%, and
64.29%, respectively, which clearly shows that the interest in this area is increasing. The
effects of various shoe characteristics on running performance and/or injury will be further
discussed below.
Theresult
Figure2.2.The
Figure result
ofof
thethe risk
risk of bias
of bias assessment.
assessment.
3. The
Figure 3.
Figure Themain
maindesign
designaspects
aspectsofof
running shoes.
running shoes.
3.3.1.The
Midsole
otherHardness
three studies emphasized the impact of hardness in different midsole areas
In the running,
on running the increase ininjuries.
performance/sports midsoleSterzing
hardnesset(i.e., from
al. [6] Asker C40
evaluated thetoeffect
AskerofC65)
running
was linked
shoes to sportsmidsole
with varying performance as specified
hardness by less and
on the rearfoot energy expended
forefoot. at thesuggested
Findings metatar- that
sophalangeal
the cushioning and less peak of
properties rearfoot eversion
the rearfoot velocity
almost [13,20].determine
entirely In contrast, theother studies
vertical ground
found that
reaction increased
forces midsoleextremity
and lower hardness kinematics.
of running shoes had no significant
Moreover, running shoes effectthat
on peak
combine
atibial
softacceleration,
forefoot andrunning speed, walking
a stiff rearfoot midsole time,
canand lower limb
effectively musclethe
improve activity (medial char-
cushioning
femoris, biceps
acteristics. femoris, and
By examining thegastrocnemius)
effects of the [5,16].
medialInand terms of running-related
lateral stiffness of theinjuries,
midsole on
findings remain inconclusive. Contradicting the general concept that
lower limb running biomechanics, Dixon et al. [21] found that the hindfoot valgus softer midsole shoesangle
and peak loading rate of ground reaction force were significantly increased whenthat
can prevent damage in running by dispersing impact mass, Baltich et al. [20] claimed running
thethe
in peak valuehardness
lateral of the vertical ground
shoes. Oriolreaction forceevaluated
et al. [17] and the stiffness of the
the effect ofknee and ankle
randomly varying
joints were
medial found reduced
dual-density as the
midsole midsole in
elements hardness of running
the running. Theyshoes increased.
concluded thatSimilarly,
although the
after tracking
midsole 247 runners
hardness for 5 the
varies from months,
lengthTheisen et al. [18]
and position, deduced
there is no that the injury
significant inci- in
difference
dence associated with applying a softer midsole might be higher
the biomechanical parameters, i.e., vertical impact force peak during running. than using a more rigid
midsole. However, the present finding indicated that regardless of the injury’s location,
type, or
3.3.2. severity,
Midsole there are no statistically significant differences between running shoes
Thickness
with varying midsole hardness.
Contrary to the previous hypothesis, different running shoe midsole thicknesses
The other three studies emphasized the impact of hardness in different midsole areas
did not change the running patterns because of the impact of relevant biomechanical
on running performance/sports injuries. Sterzing et al. [6] evaluated the effect of running
parameters (e.g., landing angle, ground reaction force). Chambon et al. [8] found that
shoes with varying midsole hardness on the rearfoot and forefoot. Findings suggested
greater midsole
that the cushioning thickness has little
properties impact
of the on kinematics,
rearfoot force,determine
almost entirely and acceleration variables,
the vertical
but
ground reaction forces and lower extremity kinematics. Moreover, running shoes that Law
it will significantly enhance the stance-phase duration. In line with this view,
et al. [12]areported
combine thatand
soft forefoot increased midsolemidsole
a stiff rearfoot thicknesscan might reduce
effectively the vertical
improve loading rates
the cushioning
and growth in By
characteristics. theexamining
contact time. Moreover,
the effects of theit medial
appears that
and footwear
lateral with
stiffness of varying midsole
the midsole
thickness has little
on lower limb effect
running on foot strike
biomechanics, pattern,
Dixon et al. stride duration,
[21] found or hindfoot
that the cadence.valgus an-
gle and peak loading rate of ground reaction force were significantly increased when run-
3.3.3.
ning inMidsole Material
the lateral hardnessand Structure
shoes. Oriol et al. [17] evaluated the effect of randomly varying
medial dual-density midsole
Ethylene-vinyl elements in(EVA)
acetate copolymer the running. They concluded
and polyurethane (PU)that
arealthough
currentlythe
widely
midsole
used hardness materials
in midsole varies fromofthe length shoes
running and position, there is no
[24]. Different significant
materials difference
have varyingincush-
the biomechanical
ioning parameters,
and resilience i.e., which
properties, verticalmay
impact force
affect peak during
running running.
efficiency and injuries. Wang
et al. [22] compared the sports performance of midsole running shoes made of EVA and
3.3.2.respectively.
PU, Midsole Thickness
The results showed that PU midsole running shoes lead to higher durabil-
Contrary
ity, while EVAtomidsole
the previous hypothesis,
running different running
shoes reinforced energyshoe midsole
returns morethicknesses did in a
than PU ones
not change
running the running
distance patterns
of 0–500 km. because of the impact of relevant biomechanical param-
eters (e.g., landing angle, ground reaction force). Chambon et al. [8] found that greater
midsole thickness has little impact on kinematics, force, and acceleration variables, but it
will significantly enhance the stance-phase duration. In line with this view, Law et al. [12]
Bioengineering 2022, 9, 497 7 of 29
Table 1. The influence of running shoes’ midsole on running performance and related injuries.
Results
Author and Year Country Experimental Shoes Participants Methods
Performance Injury
1. The influence of midsole hardness on running performance/injury
1. There is no
difference between
1. Shaw C 52 (CON) knee abduction
2. Shaw C52 + lateral peak torque and
midsole Shaw C60 10 Running speed: 3 m/s; Ground hindfoot valgus
1. LAT1 knee adduction
Dixon et al. [21] Britain (LAT1) Female running angle peak
angle ↓ (CON)
3. Shaw C52 + over 50 years old NA 2. LAT2 ground
midsole and reaction force
outsole loading rate and
hindfoot valgus
angle (CON)
1. Shaw A 40 (soft)
24 1. There is no difference in
2. Shaw A55 Running speed: 3.4 m/s; Treadmill
Hardin et al. [5] America Male peak acceleration of the NA
(medium) downhill running; Heel strike
NA tibia
3. Shaw A70 (hard)
12 1. Peak velocity of
1. Shaw A 40 (soft) Running speed: 3.4 m/s; Treadmill
Hardin et al. [14] America Male dorsiflexion of stiff ankle NA
2. Shaw A70 (hard) running; Heel strike
NA ↑ (soft)
Bioengineering 2022, 9, 497 8 of 29
Table 1. Cont.
Results
Author and Year Country Experimental Shoes Participants Methods
Performance Injury
Table 1. Cont.
Results
Author and Year Country Experimental Shoes Participants Methods
Performance Injury
1. Midsole 50 ± 1
1. SH, SS, & MM first
Asker C
peak loading rate
Soft-RF/soft-FF 1. The softer the maximum
(HH & HS)
(SS) metatarsal flexion and
2. SH second peak
2. Medium- the internal rotation
loading rate “(MM,
RF/medium-FF 28 speed ↓ (the harder)
Running speed: 3.3 ± 0.1 m/s; HH, & HS)
Sterzing et al. [6] German (MM) Male 2. MM sagittal plane
Ground running; Heel strike 3. SS second peak
3. Hard-RF/hard-FF 23.8 ± 2.0 years old landing angle ↓
loading rate” (HH
(HH) (SH&HS)
& HS)
4. Soft-RF/hard-FF 3. There is no difference in
4. MM second peak
(SH) stance time
loading rate ↓
5. Hard-RF/soft-FF
(HH)
(HS)
1. There is no
1. Shaw C, 57.02 ± difference in the
2.96 (soft) 247 male 136, female 111, Running speed: 2.61–2.69 m/s; location, type, and
Theisen et al. [18] Luxembourg 2. Shaw C, 64.47 ± NA severity of
41.8 years old Ground running; NA
2.22 (hard) running-related
injuries
Table 1. Cont.
Results
Author and Year Country Experimental Shoes Participants Methods
Performance Injury
1. Normal midsole
1. Medium-high overall
(0.76 ± 0.01
support period time &
N/mm)
pedal time ↑ (ordinary)
2. Medium hardness
2. High
midsole (7.11 ±
metatarsophalangeal
0.22 N inch mm,
19 male 25.3 ± 2.2 years Running speed: 3.5 ± 5% m/s; joint energy negative
Willwacher et al. [10] Germany 1.9 mm carbon NA
old Ground running; Heel strike function ↓ positive
fiberboard)
function ↑
3. High hardness
(normal-medium)
midsole (16.16 ±
3. There is no difference
0.20 N inch mm,
between contact time
3.2 mm carbon
and braking time
fiberboard)
Table 1. Cont.
Results
Author and Year Country Experimental Shoes Participants Methods
Performance Injury
2. The influence of midsole thickness on running performance/injury
1. There is no
1. Barefoot difference between
2. 0 mm midsole the peak value of
1. Barefoot and 0 mm
3. 2 mm midsole 15 males 23.9 ± 3.2 years Running speed: 3.3 m/s vertical ground
Chambon et al. [8] France midsole stance time ↓
4. 4 mm midsole old Ground running; NA reaction force and
(16 mm midsole)
5. 8 mm midsole the peak
6. 16 mm midsole acceleration of the
tibia
Table 1. Cont.
Results
Author and Year Country Experimental Shoes Participants Methods
Performance Injury
Note. NA: not available; LAT: lateral hardness; CON: control group; SS: soft rearfoot/ soft forefoot, soft heel/soft front palm; MM: medium rearfoot/medium forefoot, medium
heel/medium front palm; HH: hard rearfoot/hard forefoot, hard heel / hard front palm; SH: soft rearfoot/hard forefoot, soft heel/hard front palm; HS: hard rearfoot/soft forefoot, hard
heel/soft front palm, ↑indicates higher while↓indicates lower.
Bioengineering 2022, 9, 497 13 of 29
In addition to EVA and PU materials, more attention has been paid to the leaf spring-
structured midsole shoe (which replaces the traditional midsole construction by leaf spring)
in recent years. By comparing the difference between leaf spring-structured midsole
shoe (LEAF) and standard foam shoe (FOAM) on Spatio-temporal variables and running
economy, Wunsch et al. [24] found that the midsole running shoes with leaf spring structure
can significantly increase the step length, which accounts most for an improved running
economy. In the same year, the effect of a LEAF on joint mechanics and lower limb muscle
forces during overground running was contrasted to a FOAM. Wunsch et al. [23] stated that
LEAF could improve running performance by substantially reducing energy absorption at
the hip joint and energy production at the ankle joint.
Table 2. The influence of longitudinal bending stiffness on running performance and related injuries.
Results
Author and Year Country Experimental Shoes Participants Methods
Performance Injury
Table 2. Cont.
Results
Author and Year Country Experimental Shoes Participants Methods
Performance Injury
Table 2. Cont.
Results
Author and Year Country Experimental Shoes Participants Methods
Performance Injury
Note. NA: not available; CON: control; STI: stiff; STIEST: stiffest; MTPJ: metatarsophalangeal joint, ↑indicates higher while ↓indicates lower.
Bioengineering 2022, 9, 497 17 of 29
Table 3. The influence of running shoes heel-toe drop on running performance and related injuries.
Results
Author and Year Country Experimental Shoes Participants Methods
Performance Injury
1. metacarpal heel
difference 0 mm (D0) 1. BF Loading
2. metacarpal heel Running speed: rate of
12
difference 4 mm (D4) Self-selected speed; ground
Chambon et al. [11] France male NA
3. metacarpal heel Treadmill & ground reaction
21.8 ± 2.0 years old
difference 8 mm (D8) running; Heel strike force ↑ (D8)
4. barefoot (BF)
Bioengineering 2022, 9, 497 18 of 29
Table 3. Cont.
Results
Author and Year Country Experimental Shoes Participants Methods
Performance Injury
1. D6 & D0
Injury risk
of
occasional
1. metacarpal heel
runners ↓,
difference 0 mm (D0)
553 Running speed: 2.64 injury risk
2. metacarpal heel
Malisoux et al. [7] Luxembourg Male 341, female 212 m/s; Outdoor ground NA of regular
difference 6 mm (D6)
18–65 years old running; Heel strike runners ↑
3. metacarpal heel
2. There is no
difference 10 mm (D10)
difference
in overall
damage
risk.
Table 3. Cont.
Results
Author and Year Country Experimental Shoes Participants Methods
Performance Injury
Note. NA: not available; BF: barefoot, ↑indicates higher while ↓indicates lower.
Bioengineering 2022, 9, 497 20 of 29
As a brand-new “barefoot” running shoe, minimalist shoes have favored the public and
scientific researchers in the last decade. A minimalist shoe with a lower drop might result in
a biomechanical change toward a forefoot strike pattern. According to Chambon et al. [11],
the 0 mm drop shoe version generated a striking design comparable to a forefoot strike at
touchdown in the 8 mm drop shoe version. Moreover, an opposite ground reaction was
found on wearing running shoes with different shoe drops on overground and treadmill
running, which may be caused by the kinematic changes at the moment of landing.
It has been suggested that a higher shoe drop may increase knee abduction at the mid-
stance phase, influencing sagittal plane and flexion angle, and decrease tibial acceleration,
metatarsal, and knee extension angle in landing [31–33]. By investigating the influences of
shoe drop on running mechanics, Besson et al. [35] indicated that larger heel-to-toe drop
conditions would increase net knee flexion moment (p < 0.001) in the push-off phase, but
also decrease net joint ankle flexion moment during the braking phase (p < 0.001). Thus, a
more significant drop may benefit women with a stiff Achilles tendon, such as high-heeled
shoe wearers, while a shoe with no drop can be an excellent option for women who suffer
from knee pain or fatigue.
The other two articles included a long-term follow-up survey on the effects of different
shoe drops on sports performance and sports injuries. Among 553 participants examined,
Malisoux et al. [7] found no significant relationship between the overall risk of sports
injury and the difference between shoe drops of running shoes. However, low drop
running shoes were found to reduce the injury rate of occasional runners, but it seems
risky for regular runners. In the following year, Malisoux et al. [34] investigated the
long-term consequences of wearing varying height heel-to-toe drop shoes in the following
year. The results showed that apart from knee abduction during the mid-stance phase,
no discrepancies in spatiotemporal variables or kinematics were observed between shoe
versions of varying drop heights during this 6-month follow-up.
Table 4. The influence of running shoes’ weight on running performance and related injuries.
Results
Author and Year Country Experimental Shoes Participants Methods
Performance Injury
1. Barefoot;
2. Ultra-thin diving socks
3. Ultra-thin diving socks
+150 g/piece 12 1. Shoe weight increases oxygen
Running speed: 3.61
Divert et al. [36] France 4. Ultra-thin diving socks male consumption ↑ NA
m/s; Treadmill running
+350 g/piece 24.0 ± 5.0 years old
5. sports shoes 150 g/piece
6. sports shoes 350 g/piece
1. Barefoot
2. Barefoot + 150 g/piece
3. Barefoot + 300 g/piece
1. There is no difference in oxygen
4. Barefoot + 450 g/piece
consumption between bare feet
5. Nike Mayfly 14 Running speed: 3.35 and shoes
Franz et al. [37] America 6. Nike Mayfly + male m/s; Treadmill running; NA
2. Oxygen consumption increases by
150 g/piece 29.8 ± 7.3 years old Heel strike 1% for every 100 g shoe weight
7. Nike Mayfly +
increase
300 g/piece
8. Nike Mayfly +
450 g/piece
Table 5. The influence of running shoes’ heel flare on running performance and related injuries.
Results
Author and Year Country Experimental Shoes Participants Methods
Performance Injury
Table 6. The influence of running shoes’ heel stabilizers on running performance and related injuries.
Results
Author and Year Country Experimental Shoes Participants Methods
Performance Injury
16 1. HC heel
1. No heel cup (N-HC) 1. Load of plantar fascia and
Li et al. [43] China Male 10, Female 6 Ground running pain ↓
2. Heel cup. (HC) calcaneus after HC4 ↓ (N-HC)
NA (N-HC)
Note. NA: not available; NMEC: non-heel cup; HC: heel cup, ↑indicates higher while ↓indicates lower.
Bioengineering 2022, 9, 497 25 of 29
4. Discussion
The purpose of this study was to outline the impact of various sole features on running
biomechanics associated with performance and injury risk.
pattern for heel-to-toe drop since hindfoot running relies more on a positive drop while
forefoot running relies on a negative one. More studies concerning this point are warranted
for further clarification.
5. Conclusions
In summary, most of the studies have focused on investigating the impact of running
shoe midsoles, bending stiffness, and heel-to-toe drop on running performance and injuries,
while few studies on running shoe mass, heel flare, and heel stabilizer have been estab-
lished. Existing studies have initially found the impact of these structural parts on running
economy and stability, and it was found that thickness of 15–20 mm, hardness of Asker
C50-C55 of the midsole, the design of the medial or lateral heel flares of 15◦ , the curved
carbon plate, and the 3D printed heel cup may be beneficial to optimize performance and
reduce running-related injuries. Nevertheless, it is valuable to conduct more examinations
regarding these exclusive features to enhance the credibility of research results and offer
additional insights into running shoe designs. Overall suggestions for future studies are
as follows: (1) More attention on the long-term effects of running shoe constructions on
running and the underlying biomechanical mechanism of running-related injuries. (2)
Concerning the specificity, runners’ basic information should be collected (e.g., anthropo-
Bioengineering 2022, 9, 497 27 of 29
metric parameters, foot morphology, and running experience) for footwear design; (3) The
update of research and development concepts in sports biomechanics (e.g., “teeter-totter
effect” of curved carbon fiber plate) may further contribute to the development of running
shoes; (4) Footwear design and optimization should also consider the influences of runners’
strike patterns.
Author Contributions: Conceptualization, S.L. and D.S.; methodology, Y.S. and X.C.;
writing—original draft preparation, S.L., Y.S., X.C. and D.S.; writing—review and editing, K.B. and
G.F.; supervision, K.B., G.F. and D.S.; funding acquisition, D.S. All authors have read and agreed to
the published version of the manuscript.
Funding: This study was sponsored by Zhejiang Key Research and Development Program (Grant
number: 2021C03130), Zhejiang Province Science Fund for Distinguished Young Scholars (Grant
number: LR22A020002), Philosophy and Social Sciences Project of Zhejiang Province, China (Grant
number: 22NDQN223YB), Public Welfare Science and Technology Project of Ningbo, China (Grant
number: 2021S134), Basic Scientific Research Funds of Provincial Ningbo University (SJWY2022014),
and K. C. Wong Magna Fund in Ningbo University.
Institutional Review Board Statement: Not applicable.
Informed Consent Statement: Not applicable.
Data Availability Statement: All data generated or analyzed during this study are included in this
published article.
Conflicts of Interest: The authors declare no conflict of interest.
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