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negative corona

Mr. Deepak Kumar Kashyap tested positive for SARS-CoV-2 via RT-PCR with detected levels of specific RNA genes. The test was conducted on May 1, 2021, with a final report issued the same day. Clinical correlation is advised due to the potential for false positives and negatives, and further testing may be required if symptoms persist.

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41 views2 pages

negative corona

Mr. Deepak Kumar Kashyap tested positive for SARS-CoV-2 via RT-PCR with detected levels of specific RNA genes. The test was conducted on May 1, 2021, with a final report issued the same day. Clinical correlation is advised due to the potential for false positives and negatives, and further testing may be required if symptoms persist.

Uploaded by

DeepakKumarKashyap
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Patient Name : Mr.

DEEPAK KUMAR KASHYAP Collected : 01/May/2021 10:10AM


Age/Gender : 26 Y 2 M 27 D/M Received : 01/May/2021 03:01PM
UHID/MR No : CVIM.0000145106 Reported : 01/May/2021 07:24PM
Visit ID : CVIMOPV363903 Status : Final Report
Ref Doctor : Dr.SELF

DEPARTMENT OF MOLECULAR BIOLOGY.


COVID 19
Test Name Result Unit Bio. Ref. Range Method

COVID-19(SARS-CoV 2)-REAL TIME PCR(RT-PCR)


SAMPLE TYPE NASOPHARYNGEAL
SWAB AND
OROPHARYNGEAL
SWAB
SARS-CoV-2 DETECTED
RdRp Gene CT VALUE -
N-Gene CT VALUE 19
ORF1ab Gene CT VALUE 21

Comment:
Please Note:
*Not Detected (Negative) Result indicate CT Value of >35 as per the testing kit used.
*The cut-off of the reported CT value is as per the manufacturer.
*CT values vary with the type of kit used, the types of samples collected and the various pre-analytical factors.
*The published studies are conflicting to definitively prove a direct correlation between disease severity/infectiousness and CT
values therefore; relying on CT values for determining infectiousness of COVID-19 patients and deciding management protocols
is left to clinical discretion.

Kit used: CoviPath ™ COVID-19 RTPCR KIT

Result Interpretation
Indicates presence of detectable levels
Detected (Positive) Result
SARS-CoV-2 specific RNA (ORF1ab gene and/ or N gene) in patient’s sample.

Indicates absence of detectable levels


Not Detected (Negative) Result
SARS-CoV-2 specific RNA (ORF1ab gene and/ or N gene) in patient’s sample.

Indicates the target concentration is too low, or presence of interfering substances


Indeterminate Result
leading to PCR inhibition.

NOTE:

If either of the genes cannot be determined or will give indeterminate results, further testing maybe required using another kit
Page 1 of 2
Patient Name : Mr.DEEPAK KUMAR KASHYAP Collected : 01/May/2021 10:10AM
Age/Gender : 26 Y 2 M 27 D/M Received : 01/May/2021 03:01PM
UHID/MR No : CVIM.0000145106 Reported : 01/May/2021 07:24PM
Visit ID : CVIMOPV363903 Status : Final Report
Ref Doctor : Dr.SELF

DEPARTMENT OF MOLECULAR BIOLOGY.


COVID 19
Test Name Result Unit Bio. Ref. Range Method

with a different primer and probe.


A Not Detected (Negative) Resultdo not preclude SARS-CoV-2 and should not be used as the sole basis for patient
management decisions. Kindly repeat test after 48 to 72 hrs if clinically suspected.
Lower respiratory tract specimens are more representative and are preferred.
If at a later date, suspicion of COVID-19 is strong, a fresh sample for Real Time PCR after a gap of 2-4 days after collection
of first sample maybe considered.
Please contact your Physician for necessary action to be taken and to limit the spread of infection.
Clinical Correlation and Correlation with the history of the patient is required before arriving at any conclusion. Presence of
Non-Specific interfering substances during this assay to be kept in mind. Please correlate clinically before arriving at any
conclusion.
False positive and false negative results can be due to multiple factors including sampling technique, transport & interference in
the assay can affect the result.
Presence PCR inhibitors in sample may lead to false negative or invalid results.
Mutation in the target sequence of SARS-CoV-2 or change in the sequence due to virus evolution may lead to false negative
results.
Invalid Result: There is no typical S-shape amplification curve or Ct >35 or No Ct detected for target genes and internal
control, indicating the specimen concentration is too low, or there are interfering substances that inhibit the reaction. If upon
retest, the result is invalid again, another fresh sample should be collected and tested.

Disclaimer:
This Test is based on real-time reverse transcriptase PCR technology for the qualitative detection of severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2) specific RNA

*ICMR ID-AHLS001- HYDERABAD *ICMR ID-AHLVDLA- VIJAYAWADA *ICMR ID- DLUAHTMH- MUMBAI
*ICMR ID-AHLLKDLKAP- KURNOOL *ICMR ID-ADNTKLKWB - KOLKATA *ICMR ID-AHLLDLDD-DELHI
*ICMR ID-AHLLKDIALBK- BENGALURU *ICMR ID-APHLILKDLCTN- CHENNAI *ICMR ID-AHHHSPPMH- PUNE
(For kits determining RdRp gene or ORF1b, sensitivity / specificity of such kits shall be mentioned).

*** End Of Report ***

Page 2 of 2

SIN No:SWBL083222
This test has been performed at Apollo Health and Lifestyle ltd- Sadashiv Peth Pune, Diagnostics Lab

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