Glycolysis MCQs with Answer Key (BS
Level)
This document contains 100 multiple-choice questions (MCQs) related to the process of
glycolysis, specifically designed for BS-level students. Each question includes four options
and the correct answer is provided.
1. 1. Glycolysis means:
A) Breaking down protein
B) Breaking down glucose
C) Synthesizing glucose
D) Synthesizing ATP
**Correct Answer: B**
2. 2. Literal meaning of 'glycolysis':
A) Sugar production
B) Fat metabolism
C) Sugar splitting
D) ATP consumption
**Correct Answer: C**
3. 3. Site of glycolysis:
A) Mitochondria
B) Nucleus
C) Cytoplasm
D) Ribosome
**Correct Answer: C**
4. 4. Number of steps in glycolysis:
A) 8
B) 9
C) 10
D) 12
**Correct Answer: C**
5. 5. First enzyme in glycolysis:
A) Enolase
B) Hexokinase
C) Pyruvate kinase
D) Aldolase
**Correct Answer: B**
6. 6. Hexokinase adds a phosphate to glucose forming:
A) Glucose-6-phosphate
B) ATP
C) Pyruvate
D) Fructose
**Correct Answer: A**
7. 7. Conversion of glucose-6-phosphate to fructose-6-phosphate is done by:
A) Hexokinase
B) Aldolase
C) Phosphoglucoisomerase
D) Pyruvate kinase
**Correct Answer: C**
8. 8. Which enzyme uses ATP in glycolysis step 3?
A) Enolase
B) Hexokinase
C) Triose phosphate isomerase
D) Phosphofructokinase
**Correct Answer: D**
9. 9. Fructose-1,6-bisphosphate splits into:
A) Glucose and fructose
B) Pyruvate and lactate
C) DHAP and GAP
D) ATP and water
**Correct Answer: C**
10. 10. DHAP and GAP are:
A) Identical
B) Isomers
C) Opposites
D) Proteins
**Correct Answer: B**
11. 11. The enzyme that interconverts DHAP and GAP is:
A) Triose phosphate isomerase
B) Enolase
C) Aldolase
D) Kinase
**Correct Answer: A**
12. 12. Glyceraldehyde phosphate is also known as:
A) DHAP
B) Glucose
C) F-1,6-BP
D) GAP
**Correct Answer: D**
13. 13. In step 6, hydrogen is transferred to:
A) FAD+
B) NAD+
C) ADP
D) Oxygen
**Correct Answer: B**
14. 14. The first energy-yielding step in glycolysis is:
A) Step 5
B) Step 6
C) Step 7
D) Step 9
**Correct Answer: C**
15. 15. How many ATPs are generated in step 7 per glucose?
A) 1
B) 4
C) 2
D) 0
**Correct Answer: C**
16. 16. Phosphoglycerokinase catalyzes:
A) 1,3-BPG to 3PG + ATP
B) DHAP to GAP
C) Enolase reaction
D) Pyruvate to lactate
**Correct Answer: A**
17. 17. 2-phosphoglycerate is formed by:
A) Pyruvate kinase
B) Phosphoglyceromutase
C) Enolase
D) Aldolase
**Correct Answer: B**
18. 18. Enolase removes what from 2-phosphoglycerate?
A) ATP
B) NADH
C) Carbon
D) Water
**Correct Answer: D**
19. 19. Final product of glycolysis:
A) Glucose
B) ATP
C) Pyruvate
D) Citrate
**Correct Answer: C**
20. 20. Net ATP produced per glucose molecule in glycolysis:
A) 4
B) 2
C) 1
D) 3
**Correct Answer: B**
21. 21. Total ATP generated in glycolysis:
A) 2
B) 1
C) 4
D) 6
**Correct Answer: C**
22. 22. NADH produced in glycolysis:
A) 4
B) 2
C) 3
D) 1
**Correct Answer: B**
23. 23. How many pyruvate molecules per glucose?
A) 1
B) 3
C) 0
D) 2
**Correct Answer: D**
24. 24. Glycolysis is an example of:
A) Catabolic pathway
B) Anabolic pathway
C) Amphibolic pathway
D) None
**Correct Answer: A**
25. 25. Enzyme that catalyzes final ATP-generating step:
A) Hexokinase
B) Pyruvate kinase
C) Aldolase
D) TPI
**Correct Answer: B**
26. 26. Step 3 is important because it is:
A) Reversible
B) Energetically neutral
C) A rate-limiting step
D) Final step
**Correct Answer: C**
27. 27. NADH produced in glycolysis is used in:
A) Krebs cycle
B) Glycogenesis
C) Pentose pathway
D) Electron Transport Chain
**Correct Answer: D**
28. 28. Triose phosphate dehydrogenase creates:
A) NADH and 1,3-BPG
B) GAP and DHAP
C) Glucose
D) Enzymes
**Correct Answer: A**
29. 29. What is 1,3-bisphosphoglycerate classified as?
A) Amino acid
B) High-energy intermediate
C) Sugar
D) Lipid
**Correct Answer: B**
30. 30. Water removal in glycolysis occurs at:
A) Step 3
B) Step 7
C) Step 9
D) Step 1
**Correct Answer: C**
