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Alternaria brassicicola and Xanthomonas campestris

pv. campestris in organic seed production of
Brassicae: Epidemiology and seed infection

Jürgen Köhl & Jan van der Wolf

Note 363
Alternaria brassicicola and Xanthomonas
campestris pv. campestris in organic seed
production of Brassicae: Epidemiology and
seed infection

Jürgen Köhl & Jan van der Wolf

Plant Research International B.V., Wageningen

September 2005 Note 363
© 2005 Wageningen, Plant Research International B.V.
All rights reserved. No part of this publication may be reproduced, stored in a retrieval system or transmitted, in
any form or by any means, electronic, mechanical, photocopying, recording or otherwise, without the prior written
permission of Plant Research International B.V.

This literature study has been carried out in the context of the Policy Support Research Programme 'Improvement of
the quality of plant reproductive material for organic and sustainable agriculture' ('Verbetering van de kwaliteit van
plantaardig uitgangsmateriaal voor biologische en duurzame landbouw (BO-04-003)'). This Programme is financed by
the Netherlands Ministry of Agriculture, Nature and Food Quality.

Plant Research International B.V.

Address : Droevendaalsesteeg 1, Wageningen, The Netherlands
: P.O. Box 16, 6700 AA Wageningen, The Netherlands
Tel. : +31 317 47 70 00
Fax : +31 317 41 80 94
E-mail : [email protected]
Internet : www.plant.wur.nl
Table of contents

page

Epidemiology of Alternaria brassicicola in organic seed production of Brassica

Jürgen Köhl

1. Introduction 1

2. Brassica seed production: Pathogens and seed production systems 3

2.1 Role of fungal pathogens in Brassica seed production 3

2.2 Seed production systems 3

3. Damage caused by Alternaria brassicicola in Brassica 5

3.1 Seed production 5

3.2 Seedling production 5

4. Epidemiology of Alternaria brassicicola in Brassica seed production 7

4.1 Infestation of vegetative crops 7

4.2 Spread of disease 7
4.3 Survival in crop debris 8
4.4 Transmission by insects and other invertebrates 8

5. Prevention and control in organic seed production 9

6. Detection of A. brassicicola 11

7. Conclusions 13

8. References 15
Table of contents

page

Infection of Brassica seed with Xanthomonas campestris pv. campestris

Jan van der Wolf

1. Introduction 19

2. Epidemiological features 21

2.1 Initial infections 21

2.2 Dispersal of the pathogen 21

3. Seed infections 23

3.1 Invasion of the seed via systemic movement of Xcc through the vascular tissue 23
3.2 Invasion of seeds via flowers 23
3.3 Seed contamination during harvest and storage 23
3.4 Population in and on seed 24

4. Xcc in organic seed production 25

5. Literature 27
 1

Epidemiology of Alternaria brassicicola in organic

seed production of Brassica

Jürgen Köhl

1. Introduction

Alternaria brassicicola and A. brassicae are causing dark leaf spot of cultivated and wild crucifers (Smith et al.,
1988). Seeds, seedlings, leaves and pods can be damaged. Both pathogenic fungi can be seed-borne. Mycelium
can be found growing superficially on the seeds but also internally. Saprophytically surviving mycelium on crop
debris is another major inoculum source.
For the production of healthy seed, the prevention of dark leaf spot in seed crops is a pre-requisite. Foliar sprays of
fungicides are common to prevent colonization of flowers and pods by the pathogens. For seed production in
organic systems, the disease is a major threat. The use of organically produced seed not free from the disease may
lead to an increased incidence of dark leaf spot in organic production of Brassica crops.
In this literature survey, knowledge on the epidemiology of dark leaf spot in seed production of Brassica is
evaluated. This knowledge can be used for optimizing cropping systems for organic seed production with lower risks
for seed contamination by Alternaria spp. and to develop critical control points for disease management.
2
 3

2. Brassica seed production: Pathogens and

seed production systems

2.1 Role of fungal pathogens in Brassica seed

production
In vegetable Brassica seeds, A. brassicicola is the dominating Alternaria spp. (Maude & Humpherson-Jones, 1980;
Humpherson-Jones, 1985; Maude et al., 1984), whereas in oilseed rape A. brassicae is more common
(Humpherson-Jones, 1989). In Brassica vegetable production, both Alternaria spp. can occur and cause dark leaf
spot. More A. brassicae is found on Brassica vegetables in areas with oilseed rape production. Corlett & MacLatchy
(1996b) stress that A. brassicicola causes serious disease problems in vegetable production but not in oilseed rape.
Babadoost & Gabrielson (1979) surveyed 85 seed production fields of cabbage or Brussels sprouts in Western
Washington. They found A. brassicicola in 67 fields, but A. brassicae only in 6 fields. Humpherson-Jones (1983,
1985) assessed commercial Brassica seed lots in UK for infection of by Alternaria spp. and Leptosphaeria
maculans, the causal organism of blackleg, another seedborne disease. Seed lots of oilseed rape were heavily
infested by L. maculans, whereas seed lots of B. oleracea often were not infested. Since pathogenic as well as non-
pathogenic L. maculans isolates can occur in seeds, pathogenicity tests were carried out. Virulent isolates were
found on oilseed rape seed but not on seed of B. oleracea.

2.2 Seed production systems

Most research on pathogenic Alternaria spp. in seed production of Brassica is reported from England during a
period between 1980 and 1990. Crops were established by direct drilling in June or July (Maude & Humpherson-
Jones, 1980) and matured in June to August of the following year. Mature crops were cut, windrowed for drying
during several weeks and threshed.
In organic production of cabbage and other Brassica species under Dutch conditions, diseases caused by
Alternaria spp. and Mycosphaerella brassicicola are a major threat. Seed transmitted Alternaria can form a
substantial risk in seed production (Lammerts van Buren, 1994). Pathogen-free seeds can successfully be produced
in organic systems when disease-free areas are chosen for production.
Currently, Brassica seeds are produced in organic systems by a few Dutch seed companies. Various cropping
systems are used which differ in production costs and the risks for disease problems envisaged in the crop during
seed production and on the harvested seeds. Based on their experience and expectations, the representatives of
the relevant seed companies advised to focus in the project on epidemiology of diseases in organic seed production
on A. brassicicola in field grown Brassica seed crops. Special interest was expressed to obtain insight in critical
periods during which the pathogen can potentially contaminate the developing seeds externally and, more important,
internally. This knowledge can subsequently be used to develop preventive measures for use at such critical control
points. Depending on the ‘critical periods’, preventive measures may aim at manipulation of microclimatic conditions
within the crop, sanitation measures, such as removal of plant debris, and application of plant protection products
registered in organic farming or other means.
4
 5

3. Damage caused by Alternaria brassicicola

in Brassica

3.1 Seed production

Dark leaf spot disease is not restricted to leaves but can also damage fruit bearing branches and pods which turn
black when colonised by A. brassicicola. Pre-mature ripening of the pods may lead to shedding of seeds (Maude &
Humpherson-Jones, 1980). Seeds in infected pods tend to be shrunken and have low viability.
Chirco & Harman (1979) inoculated cauliflower and broccoli plants at petal fall (of oldest flowers of the plants,
flowering started at apex), mid-pod (immature pods) or ripe pod stage (seeds fully formed in ripe pods) with
A. brassicicola. Plants were inoculated at relatively high temperature (20 or 27 °C) and pods were enclosed in
plastic bags for 7 days (plastic bags opened during 7 h per day). Inoculation at petal fall stage resulted in reduced
seed weight. Germination under laboratory conditions as well as field conditions were significantly reduced for seeds
obtained from pods treated with A. brassicicola at petal fall or mid-pod stages. Inoculation of ripe pods did not affect
field emergence of seeds. Seed infection by A. brassicicola was increased after inoculation of plants with the
pathogen in comparison to seed from plants only treated with water. Highest seed infections were found on plants
inoculated early at petal fall. Surface sterilisation reduced seed infection in many, but not in all cases. The
experiment of Chirco & Harman (1979) was carried out under conditions of artificially high humidity combined with
high temperatures during and after inoculation with A. brassicicola. Furthermore, seeds were harvested and
threshed after pod maturation. It cannot be concluded from the data at which stage seeds within pods were infested
by A. brassicicola during maturation.
Superficial contamination of seed surfaces by necrotrophic pathogens is more common than internal colonisation of
seeds after infection (Maude, 1996). External inoculum can be located in cracks of the seed coats so that conidia
are protected from adverse environmental conditions but also from physical seed treatments. More external
colonisation is found in the area of the seed hilum which can be explained by mycelial growth of the pathogen
through the suture of silicas (pods) and the funicles (Maude, 1996).
Internal infection of seeds by A. brassicicola was found in 89 out of 139 samples of basic seeds during an inventory
carried out in 1976-1978 in the UK (Maude & Humpherson-Jones, 1980). On average 4.9% of the seeds were
internally infected. In the same study, 96% out of 112 assessed commercial seed lots were infested, on average
18% of the seeds were internally infected. Seed lots of cabbage with internal infection by A. brassicicola were also
assessed for external inoculum. Up to 500 conidia per seed were found. There was a strong correlation between the
number of external conidia per seed and the incidence of internally infected seeds.
Within internally infected seeds, A. brassicicola is mainly found in seed coats (Maude & Humpherson-Jones, 1980). In
heavily infested seed lots, also the cotyledon tissue of the embryo can be infected. Interestingly, the incidence of
internal infection was similar for small, shriveled seeds and large, round seeds, so that a selection of healthy seeds
on the basis of appearance and size was not possible. Both internal and external inoculum can survive for several
years. Maude & Humpherson-Jones (1980) state that longevity of internal inoculum is much higher than of external
inoculum.

3.2 Seedling production

Germinating Brassica seeds are susceptible to infection by A. brassicicola by conidia contaminating the seed
surface (Knox-Davies, 1979). After rupture of the testa, germination of conidia is stimulated and especially the hilum
area and damaged parts of the testa can be infected by the pathogen. Immature seeds are more vulnerable than
mature seeds.
Seedlings developed from infected seeds show typical symptoms of small discrete dark spots on the under-surface
of the cotyledons or dark stripes on the hypocotyls. Seedlings from heavily infected seeds often die when tested in
the laboratory. Under greenhouse or field conditions, symptom development is generally less severe than under lab
conditions and damping-off caused by A. brassicicola is less common, e.g. 19% of seedlings developed from
6

contaminated seeds showed disease symptoms when sown in the greenhouse and 6% showed symptoms under
field conditions. Cotyledon infection under field conditions was often associated with seed coats sticking to
cotyledons during emergence (Maude & Humpherson-Jones, 1980).
Maude & Humpherson-Jones (1980) found a significant correlation between internal seed infestation and symptom
development on seedling under field conditions. However, surface sterilisation reduced the number of infected
seedlings. The experimental data do not allow a clear conclusion on the importance of external versus internal
inoculum.
Infection of seedlings by A. brassicicola after artificial inoculation of hypocotyls or cotyledons or naturally infested
seeds depended on incubation temperature (Bassey & Gabrielson, 1983a). Optimum temperature for development
of wirestem symptoms was 25 °C. Little wirestem symptoms occurred at temperatures below 20 °C.
 7

4. Epidemiology of Alternaria brassicicola in

Brassica seed production

4.1 Infestation of vegetative crops

Crops for seed production can be infested by A. brassicicola from infected seeds, infested crop debris present in
the field or transferred by wind from infested mature crops. Especially during cutting and trashing of windrowed
crops huge amounts of conidia were released and captured at distances of up to 1800 m in spore traps consisting
of Petri dishes containing a selective medium (Humpherson-Jones & Maude, 1982a). It was concluded that sufficient
distances and optimum regional distribution of vegetative and maturing seed crops may prevent early infestation of
vegetative seed crops. In modern production systems, especially in areas with low temperatures during winter,
vegetative crops are not established by seeding in the field, but transplants are produced in greenhouses or tunnels
during winter and transplanted in the field in spring. In such a system, inoculum produced on mature crops is no
threat for the new crops since there are no overlapping growing seasons. Conidia of A. brassicicola formed on crop
residues or alternative hosts such as weeds may play an important role as inoculum source in such cropping
systems but no quantitative data are available on this aspect. The disease may also enter the vegetative crop phase
during seedling production from infested basic seed. Transmission of A. brassicicola from infested seed to seedling
was investigated by Maude & Humpherson-Jones (1980). Under glasshouse conditions, 12-19% of infested seeds
produced seedlings with symptoms. However, the rate of disease transmission was lower under field conditions.
Even from seed which was affected by the pathogen for 100%, only 6% of the developing seedlings showed
symptoms of disease.

4.2 Spread of disease

In overwintering Brassica seed crops, A. brassicicola can survive on the litter. Humpherson-Jones & Maude (1982a)
found several hundred up to a maximum of 40.000 conidia of A. brassicicola produced per g of overwintered litter
when sampled in April. These conidia are considered the primary inoculum inciting epidemics in seed crops.
Conidia were also sampled in the air above crops during two growing seasons. There was a slow increase of air load
with A. brassicicola conidia during the season with significant peaks when the crops were cut and harvested with a
maximum spore load of 12.000 conidia per m3 air. During the growing season conidia were mainly found on dry
days following periods of rain with long leaf wetness periods. Most spores were released during the day when
relative humidity decreased in the crop, the minimum number of conidia was released during the moist periods at
night. This diurnal periodicity of spore release was also found by Kennedy et al. (1999) and Chen et al. (2003). In a
study in oilseed rape, spore release was observed mainly during 7.00 and 21.00 h, with a maximum spore release
during 13.00 and 15.00 h (Kennedy et al., 1999). Sporulation and spore release in the field could be predicted by
using models based on temperature and humidity. Chen et al. (2003) found diurnal periodicity of spore flights with
peaks at 10:00 h. Spore release and flights were mainly affected by rain and wind. Spores were spread over short
distances, so that it can be assumed that they retain within the crop canopy (Chen et al., 2003). Spread over longer
distances of up to 1800 m was found only during trashing of infested crops (Humpherson-Jones & Maude, 1982a).
Spore trapping at different heights showed that most conidia were transported over short distances of several
centimeters resulting in a gradient in spore load within the canopy from bottom to top (Humpherson-Jones & Maude,
1982a; Chen et al., 2003). Disease development started on pods close to the soil earlier in the season and
progression was faster compared to pods at 1.25 or 2.5 m canopy height. This can be explained by the different
inoculum level in combination with a more favourable micro-climate in the lower canopy levels. The effect of early or
late infection of pods on the infection of seeds has not been followed in this study.
The spread of dark leaf spot caused by A. brassicicola in cabbage crops was studied by Fontem et al. (1991). They
found steep gradients of disease severity in distance from infected plants used as inoculum source. This pattern of
the spread of the disease within the crop indicated a short distance dispersal of conidia. Also Chen & Price (2002)
found a short distance spread of the disease in Chinese cabbage.
8

A detailed study was carried out by Humpherson-Jones & Maude (1982a) to follow the disease development in the
period between cutting and threshing after windrowing. In general, seeds threshed at cutting showed less infection
compared to seeds of windrowed plants. Surface sterilisation of seeds was not included in this study. Thus, it cannot
be concluded from the data whether external contamination increased on seeds from windrowed plants due to a
higher inoculum load produced on the pods during windrowing and contaminating seeds externally during threshing.
Seeds may also be infected internally during the period of windrowing.

4.3 Survival in crop debris

The capacity of leaf and stem debris of cabbage to serve as inoculum source of A. brassicicola was assessed by
Humpherson-Jones (1989). Leaves were collected in seed production crops of cabbage infested with A. brassicicola
and A. brassicae, placed on soil outdoors for several weeks and the potential of both Alternaria spp. to produce
conidia on the leaves in moist chamber was measured regularly. Conidia were produced on leaves which had been
exposed to field soil for up to 12 weeks. On average approximately 105 conidia of both Alternaria spp. were formed
per leaf. On infested stem segments, conidia production was observed even after 23 weeks of exposure to field soil.
Approximately 103 conidia per cm stem segment were produced. The viability of conidia produced on leaf or stem
debris was higher than 75%. Since no observations on sporulation capacity on cabbage stems were made after field
exposure for periods longer than 23 weeks, there is no information provided on the maximum period during which
Alternaria spp. are able to produce conidia on stem debris. Survival of Alternaria spp. in stem debris may be
different for stems on soil surface or ploughed in the soil. Stem debris ploughed up to the soil surface in subsequent
years may still contain Alternaria inoculum. It is concluded by Humpherson-Jones (1989) that crop debris is the
major inoculum source of A. brassicicola and A. brassicae in areas in which the disease is established. However,
long-term survival of both pathogens in debris has not been investigated.

4.4 Transmission by insects and other invertebrates

Various pest insects, such as pollen beetle and seed pod weevil, are feeding on Brassica spp. or may lay eggs in
flowers and young pods which later serve as feed for larvae. Flowers of Brassica spp. are also visited by various
beneficial insects playing an important role in pollination. In seed production, bees, bumble bees, but also flies are
used to assure optimum pollination in production systems in the open field, tunnels or greenhouses.
Such beneficial or pest insects may act as vectors of fungal spores. Quak (1956) investigated under field conditions
the possible role of pollen beetles (Ceuthorrhynchus assimilis) and seed pod weevils (Meligethes aeneus) as vectors
of Alternaria spp. in oilseed rape. Presence of the pests in cages placed in the crop did not increase disease
development on pods in comparison to pods from plants in cages without pests. Similar results were obtained at
two locations.
Dillard et al. (1998) observed flea beetles (Phsyllotreta cruciferae) in cabbage fields feeding on plants infected by
A. brassicicola. A brassicicola could be isolated from 20 to 30% of beetles caught in such fields. Viable pathogen
conidia were found externally on the insect surface, mainly in the cavities of the exoskeleton, but also in the
digestive tracts and beetle feces. The percentage of beetles with A. brassicicola increased during the growing
season. Beetles collected in diseased fields were kept in cages with healthy cabbage plants. Disease was
transmitted to plants grown under greenhouse. Development of leaf spots was as severe as on plants artificially
inoculated with conidial suspensions of A. brassicicola, whereas untreated plants were almost free of disease
symptoms.
Transmission of A. brassicicola by slugs has also been reported (Hasan & Vago, 1966) who observed feeding of
slugs on infected cabbage leaves. Viable conidia of A. brassicicola were found in the excrements of slugs during a
period of one week after being fed with infected leaves.
 9

5. Prevention and control in organic seed

production

Transmission of the disease from mature crops to vegetative crops can be prevented if crops are not established by
drilling in the field but by planting transplants in spring. If crops are established by seeding, the distance between
mature crops and newly seeded crops should be more than 1800 m (Humpherson-Jones & Maude, 1982a).
In integrated seed production, seeds are treated by fungicides (Maude et al., 1984) and repeated foliar applications
of fungicides, e.g. iprodione, are common to protect pods from infection. Seeds obtained from treated plants are
significantly less infected by A. brassicicola if the disease was present in the field (Humpherson-Jones & Maude,
1982b). Since synthetic fungicides are not available in organic production, basic seed should be produced in
disease-free areas (Lammerts van Buren, 1994) and tested for absence of the pathogen. However, if only pathogen-
infested basic seed is available, seed treatments, e.g. with hot water, have to be developed.
Given the high temperature optimum for seedling infection (Bassey & Gabrielson, 1983a), climate control in
production of seedlings is important. Temperatures above 20 °C should not occur in combination with humid
conditions.
Humpherson-Jones & Phelps (1989) found that the sporulation process of A. brassicicola is inhibited by dry periods
of at least 3 h. Experiments carried out under controlled conditions showed that A. brassicicola can sporulate in a
wide temperature range between 5 and 30 °C, if the relative humidity is above 90% (Humpherson-Jones & Phelps,
1989). A moist period of at least 12 h is needed to complete sporulation. If a moist period was interrupted by a dry
period at 70 or 80% r.h. for a few hours, the sporulation process was disturbed and was not continued in a following
moist period. Several generations of spores can be produced on tissues given sufficiently long wetness periods of
>12 h. Dry periods between such long moist periods did not affect the spore yield in subsequent sporulation
periods. Climate control in the seed crop can thus be an important means to prevent sporulation of the pathogen on
infected tissue by achieving short durations of wetness periods within the crop.
In field experiments with artificially inoculated plants serving as inoculum source in a crop of Chinese cabbage,
Chen & Price (2002) found that the disease spread was driven by wind. More disease was found downwind of the
inoculum source. The disease spread faster within rows than across rows. Orientation of rows not parallel but in
right angles to the main wind direction was suggested to reduce the risks of disease development.
Protection of Brassica leaves from infection by A. brassicicola using antagonistic fungi has been investigated by
Pace & Campbell (1974). Applications of Aureobasidium pullulans and Epicoccum nigrum on leaves reduced
infection under controlled conditions. However, biological control of A. brassicicola under field conditions has not
been reported.
10
 11

6. Detection of A. brassicicola

A. brassicicola can be identified and distinguished from A. brassicae and A. alternata based on the appearance of
the conidia (Corlett & MacLatchy, 1996a, b; Mathur & Kongsdal, 2003; Simmons, 1995). Maude & Humpherson-
Jones (1982a) used prune lactose agar on which A. brassicicola can be distinguished from A. brassicae based on
colony characteristics.
Prune lactose yeast agar supplemented with benomyl and DuPont exp fungicide DPX 3217 (Humpherson-Jones &
Maude, 1982a) has been used as semi-selective medium, e.g. for spore trapping in the field. For seed testing,
Wu & Chen (1999) developed a semi-selective medium. On this CW-medium, growth of various fungi is suppressed.
Colonies of A. brassicicola have a powdery appearance and can easily be distinguished macroscopically from other
Alternaria spp., including A. alternata (M. Asma, pers. communication) which is not possible on ARSA (Pryor et al.,
1994), another medium semi-selective for Alternaria spp. (C. van Tongeren, pers communication).
Seeds can be assessed using various tests, e.g. blotter method, deep freezing blotter method, agar plate method,
tests on filter paper based on the use of 2,4-D to prevent seed germination, or germination tests (Anon. 1966;
Bassey & Gabrielson, 1983b; Vannacci, 1981). Optimum temperature to detect A. brassicicola on infested seeds
was 20 to 25 °C.
PCR-based detection of A. brassicicola on cruciferous seeds was developed by Iacomi-Vasilescu et al. (2002).
The designed primer pairs were highly specific for the pathogen and could be used for detection of presence of
A. brassicicola in seeds with infection levels of 10% or higher. The quantitative detection of A. brassicicola in host
tissue of Arabidopsis by real-time PCR has been demonstrated by Brouwer et al. (2003) and Gachon & Saindrenan
(2004). However, in these studies other Alternaria spp. were not present in the plant tissues and no information is
given on the specificity of the used primers. Blast analysis of sequences of primers used by Brouwer et al. (2003)
showed that these were not specific for A. brassicicola but were also found in strains of various Alternaria spp.,
Ulocladium spp. and other fungi (P. Bonants, pers. communication). Primers used by Gachon & Saindrenan (2004)
are based on sequences of Cutinase A (ABU03393) gene of A. brassicicola. A blast analysis does not allow an
estimation of the specificity of these primers because of the limited data in the data base. It is likely that other
Alternaria spp. may contain cutinase genes too. For the application of real-time PCR for quantification of
A. brassicicola in plant tissues sampled in the field or in seeds, primers with a high specificity are needed because
various fungi, including those closely related to A. brassicicola, may be present. The primers published by Iacomi-
Vasilescu et al. (2002) for A. brassicicola are not suitable for real-time TaqMan-PCR because the amplicon is too
large and the forward primer partly overlaps with an ITS area most specific and thus ideal for probe development
(R. van Hoof, pers. communication). For A. brassicae, such specific primers for specific and quantitative detection
using real-time PCR have been developed by Guillemette et al. (2004).
12
 13

7. Conclusions

Little information is available on organic production systems for seed production of Brassica, especially on specific
problems caused by diseases and their prevention. From the information collected from literature in this review, the
following measures for prevention of seed contamination by A. brassicicaola can be advised:
• Production of pathogen-free basic seed in disease-free areas;
• Thorough seed testing to ensure pathogen-free basic seeds;
• Climate control during seedling production avoiding temperatures above 20 °C in combination with humid
conditions;
• Seed production fields at distance of at least 2 km from other Brassica crops or crop residues including
oilseed rape;
• Establishment of crops with open canopy to reduce risks of long wetness periods;
• Control of pollen beetle, flea beetle and slugs as potential vectors;
• Control of weeds within the crop and wild plants in neighbourhood of crop which may serve as host;
• Monitoring of diseases in crop and removal of infected plants or plant parts;
• Removal of crop residues.

Epidemiological research on A. brassicicola in organic seed production of Brassica should be directed at

contamination of seed during their development in the field by locally produced inoculum. For such studies, the
quantitative specific detection of A. brassicicola in the crop will be an important tool. Based on the results of such
studies, measures can be developed for disease prevention or control in organic seed production of Brassica, such
as climate control in the crop, disease control with fungicides available in organic farming during critical periods or
vector control.
14
 15

8. References

Literature was collected from the following sources: Biological Abstracts, Current Contents, CABI abstracts, organic
eprints (www.orgprints.org) and the literature collection of C.J. Langerak.

Anonymous, 1966.
International rules for seed testing. Proceedings of the International Seed Testing Association 31: No. 1,
p. 111.
Babadoost, M. & R.L. Gabrielson, 1979.
Pathogens causing Alternaria diseases of Brassica seed crops in Western Washington. Plant Disease
Reporter 63: 815-820.
Bassey, E.O. & R.L. Gabrielson, 1983a.
The effects of humidity, seed infection level, temperature and nutrient stress on cabbage seedling disease
caused by Alternaria brassicicola. Seed Science and Technology 11: 403-410.
Bassey, E.O. & R.L. Gabrielson, 1983b.
Factors affecting accuracy of 2,4-D assays of crucifer seed for Alternaria brassicicola and relation of assays
to seedling disease potential. Seed Science and Technology 11: 411-420.
Brouwer, M., B. Lievens, W. van Hemelrijk, G. van den Ackerveken, B.P.A. Cammue & B.P.H.J. Thomma, 2003.
Quantification of disease progression of several microbial pathogens on Arabidopsis thaliana using real-time
fluorescence PCR. FEMS Microbiology Letters 228: 241-248.
Chen, L.Y. & T.V. Price, 2002.
Dark leaf spot (Alternaria brassicicola) on Chinese cabbage: temporal spread and its influencing factors.
Australian Journal of Agricultural Research 53: 1095-1103.
Chen, L.Y., T.V. Price & Z. Park-Ng, 2003.
Conidial dispersal by Alternaria brassicicola on Chinese cabbage (Brassica pekinensis) in the field and under
simulated conditions. Plant Pathology 52: 536-545.
Chirco, E.M. & G.E. Harman, 1979.
The effects of Alternaria brassicicola infection on Brassica seed vigor and viability. Journal of Seed
Technology 3: 12-22.
Corlett, M. & I.A. MacLatchy, 1996a.
Fungi Canadenses No. 334: Alternaria brassicae. Canadian Journal of Plant Pathology 18: 482-483.
Corlett, M. & I.A. MacLatchy, 1996b.
Fungi Canadenses No. 335: Alternaria brassicicola. Canadian Journal of Plant Pathology 18: 484-485.
Dillard, H.R., A.C. Cobb & J.S. Lamboy, 1998.
Transmission of Alternaria brassicicola to cabbage by flea beetles (Phyllotreta cruciferea). Plant Disease
82: 153-157.
Fontem, D.A., R.D. Berger, D.P. Weingartner & J.A. Bartz, 1991.
Progress and spread of dark leaf spot in cabbage. Plant Disease 75: 269-274.
Guillemette, T., B. Iacomi-Vasilescu & P. Simoneau, 2004.
Conventional and real-time PCR-based assay for detecting pathogenic Alternaria brassicae in cruciferous seed.
Plant Disease 88: 490-496.
Hasan, S. & C. Vago, 1966.
Transmission of Alternaria brassicicola by slugs. Plant Disease Reporter 50: 764-767.
Humpherson-Jones, F.M., 1983.
The occurrence of Alternaria brassicicola, Alternaria brassicae and Leptosphaeria maculans in brassica seed
crops in south-east England between 1976 and 1980. Plant Pathology 32: 33-39.
16

Humpherson-Jones, F.M., 1985.

The incidence of Alternaria spp. and Leptosphaeria maculans in commercial brassica seed in the
United Kingdom. Plant Pathology 34: 385-390.
Humpherson-Jones, F.M., 1989.
Survival of Alternaria brassicae and Alternaria brassicicola on crop debris of oilseed rape and cabbage.
Annals of Applied Biology 115: 45-50.
Humpherson-Jones, F.M. & R.B. Maude, 1982a.
Studies on the epidemiology of Alternaria brassicicola in Brassica oleracea seed production crops. Annals of
Applied Biology 100: 61-71.
Humpherson-Jones, F.M. & R.B. Maude, 1982b.
Control of dark leaf spot (Alternaria brassicicola) of Brassica oleracea seed production crops with foliar sprays
of iprodione. Annals of Applied Biology 100: 94-104.
Humpherson-Jones, F.M. & K. Phelps, 1989.
Climatic factors influencing spore production in Alternaria brassicae and Alternaria brassicicola. Annals of
Applied Biology 114: 449-458.
Gachon, C. & P. Saindrenan, 2004.
Real-time PCR monitoring of fungal development in Arabidopsis thaliana infected by Alternaria brassicicola and
Botrytis cinerea. Plant Physiology and Biochemistry 42: 367-371.
Iacomi-Vasilescu, B., D. Blancard, M. Guénard, V. Molinero-Demilly, E. Laurent & P. Simoneau, 2002.
Development of a PCR-based diagnostic assay for detecting pathogenic Alternaria species in cruciferous
seeds. Seed Science and Technology 30: 87-95.
Kennedy, R., K. Phelps & A.J. Turner, 1999.
Prediction of sporulation by Alternaria brassicae and A. brassicicola on Brassica napus. Proceedings of the
10th International Rapeseed Congress, Canberra, Australia; www.regional.org.au/au/gcirc/3/390.htm.
Knox-Davies, P.S., 1979.
Relationships between Alternaria brassicicola and Brassica seeds. Transactions of the British Mycological
Society 73: 235-248.
Lammerts van Buren, E., 1994.
Zaaizaadvermeerdering in de biologische groenteteelt. Louis Bolk Instituut, Driebergen, the Netherlands.
Mathur, S.B. & O. Kongsdal, 2003.
Common laboratory seed health testing methods for detecting fungi. ISTA, Copenhagen, Denmark.
Maude, R.B., 1996.
Seedborne diseases and their control: principles and practice. CAB International, Wallingford.
Maude, R.B., F.M. Humpherson-Jones & C.G. Shuring, 1984.
Treatments to control Phoma and Alternaria infections of brassica seeds. Plant Pathology 33: 525-535.
Maude, R.B. & F.M. Humpherson-Jones, 1980.
Studies on the seed-borne phases of dark leaf spot (Alternaria brassicicola) and grey leaf spot (Alternaria
brassicae) of brassicas. Annals of Applied Biology 95: 311-319.
Pace, M.A. & R. Campbell, 1974.
The effect of saprophytes on infection of leaves of Brassica spp. by Alternaria brassicicola. Transactions of
the British Mycological Society 63: 193-196.
Pryor, B.M., R.M. Davis & R.L. Gilbertson, 1994.
Detection and eradication of Alternaria radicina on carrot seed. Plant Disease 78: 452-456.
Quak, F., 1956.
De biologie en de bestrijdingsmogelijkheden van de veroorzakers van spikkelziekte (Alternaria spec.) in
koolzaad (Brassica napus L.). Verslagen van Landbouwkundige Onderzoekingen No. 62.8, Staatsdrukkerij
Uitgeversbedrijf, ‘s-Gravenhage.
Simmons, E.G., 1995.
Alternaria themes and variations (112-114). Mycotaxon 55: 55-163.
Smith, I.M., J. Dunez, R.A. Lelliott, D.H. Phillips & S.A. Archer, 1988.
European Handbook of Plant Diseases. Blackwell Scientific Publications, Oxford, UK.
 17

Vannacci, G., 1981.

Seed-borne Alternaria brassicicola: Detection by means of symptoms on seedlings. Acta Horticulturae
111: 123-129.
Wu, W.-S. & T.-W. Chen, 1999.
Development of a new semiselective medium for detecting Alternaria brassicicola in cruciferous seeds.
Seed Science and Technology 27: 397-409.
18
 19

Infection of Brassica seed with Xanthomonas

campestris pv. campestris
Jan van der Wolf

1. Introduction

Black rot of crucifers is characterized by blackened vascular tissues and foliar marginal V-shaped chlorotic or
necrotic lesions (Cook et al., 1952a). As the disease progresses, parenchyma cells surrounding vessels in the main
stem turn black, and the plant becomes wilted, stunted and finally rots. The disease is found throughout the world
and is one of the most destructive diseases of crucifers which cause considerable economic damage.

The disease is caused by the Gram-negative bacterium Xanthomonas campestris pv. campestris, (Xcc), a pathogen
that can infect a wide range of plants within the crucifer family Brassiceae, including cabbage, cauliflower, kale,
rape, radish, and black mustard, but also Arabidopsis, a plant used in molecular model studies. Epidemics caused
by Xcc are polycyclic. The pathogen, which once established, repeatedly multiplies and spreads when conditions are
favorable (Figure 1).

Seed bed Production field

Planting

(Symptomless)
spread in seedbed Secundary
Infected plants infection
survival in Seed
weeds in the field
infection
seedling symptoms
Survival in plant
debris or winter
seed cabbage
Survival in plant debris or
winter cabbage
Seed infection

Figure 1. Disease cycle of black rot caused by Xanthomonas campestris pv. campestris in cabbage. For initial
infections, debris of cabbage plants, weeds and introduction via irrigation water are most important.
Dispersal in nurseries and in the fields are mainly due to splash dispersal.

The pathogen has a high degree of variability with respect to the virulence on host plants, serology, genetics, and
with respect to physiological and biochemical properties (Alvarez et al., 1994). A leaf spot disease of crucifers is
caused by a closely related seedborne pathogen, X. c. pv. armoraciae (McCulloch 1929) Dye 1980, which is
equivalent to X. campestris pv. raphani (Machmud, 1982).

In this survey, literature is extracted concerning infection of Brassica seeds with Xcc. First, important
epidemiological features of Xcc are outlined briefly. Thereafter, routes for seed infections and the localization of Xcc
within seeds in relation to disease incidence are summarized. The survey finalizes with some remarks concerning
the risks for seed infections with Xcc in organic agriculture.
20
 21

2. Epidemiological features

2.1 Initial infections

Diseases often start with infected seed as the initial inoculum, even though infected seeds often appear healthy
(Cook et al., 1952b). Other primary sources of infection can be soil, splashes and aerosols dispersed from adjacent
infected fields, infected (perennial) weeds, infected machineries and materials and possibly insects.

Soil is a well-known source of inoculum. Xcc can only survive for 20-50 days free in soil, but for ca. two year in
cabbage residues in soil (Schaad & White, 1974; Dzhalilov & Tiwari, 1995; Kocks et al., 1998)

Aerosols were taken from a field with Xcc infected Brassica campestris weed plants using a particle sampler, both
during rainy and dry periods. The number of cfu per cubic meter of air ranged from ca. 14 during rain to ca. 1
during dry periods indicating that Xcc can be dispersed from infected weeds (Kuan et al., 1986).

Several weeds, in particular cruciferous weeds, have been found to host Xcc (Schaad and Dianese, 1981; Kuan
et al., 1986; Dane and Shaw, 1996). During a survey in the USA (Georgia and California) it was found that Xcc is
readily transmitted from infected weeds to cabbage under field conditions. Infection of weeds was not always
associated with cultivated crop of crucifers. They were even found 32 km from the nearest cultivated crucifer crop.
Epiphytic populations on weeds were lower after periods of heavy rainfall and low temperatures at night (0.6-6 oC).

The role of insects is largely unknown. Only some transmission studies were done with flea beetles (Phyllotreta
cruciferae) (Shelton and Hunter, 1985). Beetles infected by feeding them for 48 h on cabbage plants showing black
rot symptoms, were able to transfer Xcc to broccoli plants in greenhouse experiments with a high efficiency.
However, black rot did not develop when immigrating flea beetles were collected in cabbage fields and transferred
to healthy broccoli plants in the green house. Insects are attracted by the nectar of blooming plants and may easily
infect the flowers and from there the developing seed.

2.2 Dispersal of the pathogen

During germination of infected seeds, the pathogen invades the mesophyll and the vascular tissues of the epicotyl,
infects foliage and is released through guttation droplets at leaf margins. During the cropping period, inoculum is
spread by watersplash, wind-driven rain, aerosols, possibly insects and by mechanical injury during cultivation
(Williams, 1980; Kuan et al., 1986). In particular Xcc will rapidly spread in misted seedbeds from infected seedlings
(Roberts et al., 1999; Shigaki et al., 2000).

After bacteria are deposited on the leaf surface, the epiphytic populations may build up, such as have been
demonstrated for other Xanthomonads (Rudolph et al., 1994). They can remain on the leaf surface at relatively high
densities (> 100 cfu/cm2) for more than 40 days (Dane & Shaw, 1993; Arias et al., 1996). Xcc is protected from
photobiological damage by the yellow pigment, xanthomonadin (Poplawsky and Chun, 1998). Xcc colonizes plant
surfaces in aggregates.

Plants are mainly invaded through hydathodes and occasionally via leaf and root wounds and other natural openings
such as stomates and the openings at root junctions. Hydathodes (water pores) provide a continual path of water
from vessels to leaf margins. The water contains minerals, carbohydrates and amino acids (glutamine) and has a pH
of 7.0, which is optimal for growth of Xcc. Cells probably loss motility when they colonize the leave surface, but as
soon as free water becomes available, they shift to a motile phase to reach the leaf margins, due to chemotaxis.
When they have colonized the guttating leaf margins they become non-motile again (Kamoun and Kado, 1990).
Stomatal infections are rarely seen in the field, but occasionally are observed in seed beds under conditions that
reduce hydrophobicity of the cells, e.g. after treatment with crop protection agents. Stomatal infection may result in
22

a leaf spot disease. Roots may become colonized from infected plant debris in soils. Root infections have resulted in
extensive colonization of plants and typical V-shaped lesions on the leaves, indicating that this infection route is also
possible (McElhaney et al., 1998). Bacterial growth and symptom expression is strongly favored by temperatures of
25-30 oC and they are masked at 15-20 oC.
 23

3. Seed infections

Cabbage seed is produced primarily in temperate regions, where cool mild winters are found for vernalization. Seed
production starts in midsummer in seed beds and seedlings are transplanted to production fields roughly after a
month. In spring, plants bolt to flower. In summer seeds ripen and are harvested. The seed production is most
susceptible to infection with Xcc during the late summer in seed beds, before transplanting and just before
flowering, because in these periods the temperatures are most favorable for bacterial growth.

3.1 Invasion of the seed via systemic movement of Xcc

through the vascular tissue
On infected seed plants grown under optimal conditions for seed production, black rot symptoms develop in general
after the flowering stage is well advanced (Cook at al, 1952b). At that time the vascular system of branches,
pedicels and pods is systemically infected. Xcc moves through the infected pods to the funiculi. From there, the
invasion of the seed coat is likely, although it has not been demonstrated histologically. In seeds with infected
funiculi and seed coats Xcc likely is able to resist chemical- and hot water treatments.

3.2 Invasion of seeds via flowers

For several plant pathogens flowers have been recognized as an important port of entry. Erwinia amylovora, the fire
blight pathogen, can enter unwounded blossoms through natural openings in the stigma, nectary, anthers and sepals
of pear (Van der Zwet et al., 1988). Pollinating bees and other insects play an important role in these flower
infections. Flower infections have also been proven for bacterial blossom blight of kiwifruit caused by a
Pseudomonas sp., for Pseudomonas syringae in pear and for Acidovorax avenae subsp. citrulli in watermelon
(Ercolani, 1970; Morita, 1995; Walcott et al, 2005).
Surprisingly, no literature on the role of flower infections in the epidemiology of Xcc is available. In most cabbage
varieties, insects play a crucial role in pollination. Therefore it can be hypothesized that contaminated bees, bumble
bees, flies but also pest insects may readily spread the Xcc when visiting flowering cabbage plants. The potential of
insects to transmit bacteria may also be used for delivering of biocontrol agents, as have been demonstrated for
honey bees contaminated with Bacillus subtilis, which pollinated blueberry flowers (Dedej et al., 2004).

3.3 Seed contamination during harvest and storage

External seed contamination probably occurs mainly during threshing, during which contact with infected pods,
stems and leaves may result in seed contaminations. Further smearing may occur during seed grading and storage
handlings. In general this will result in an external seed contamination. It is not exactly known to what extent external
contaminations contribute to seedling infections. Experiments at Bejo Seeds with surface sterilized seeds and
untreated seeds indicated that mainly seeds internally colonized result in disease expression and transmission (V.d
Heijden, personal communication). Bacterial populations superficially present on seed coats will decrease during
storage and in particular after sowing in soil, due to physical factors, predation and antagonism. Bacteria that
persist may however enter and colonize the germinating seeds and emerging seedlings. The exact role of the
externally located seed populations is unknown.
24

3.4 Population in and on seed

According to Clayton (1925), Xcc can survive for a period of 3 years in seed, although the percentage of seeds with
viable bacteria decreased with age of the seed. However, for many other bacteria, including other Xanthomonas
species a survival period of 2-3 years have been described for populations on the seed surface, but prolonged
survival times for bacteria in the seed (Neergaard, 1979). It is therefore likely that in the seed, Xcc may survive for
longer periods than 3 years. It is expected that the Xcc internally present will survive better than on the outside.
The percentage of infected seed is generally small (<0.1%) and rarely exceeds 1%. In the initial stages of infection,
the percentage infected seeds per seed lot relates relatively well with the number of infected plants (Schaad et al.,
1980).

Under favorable conditions for disease expression, a percentage of 0.03% seed infection can cause black rot in the
field already (Schaad et al., 1980). At initial stages of an epidemic, symptom expression is mainly dependent on the
percentage of seed infection and the densities per seed (Roberts et al., 1999). In later stages the environmental
conditions, under which the water regime, determines disease expression more.

The distribution of pathogenic bacteria in seeds seems to follow a lognormal or a Poisson distribution (Gitaitis et al.,
2005). Densities of X. axonopodis pv. vignicola in cowpea, Clavibacter michiganensis subsp. michiganensis in
tomato and Acidovorax avenae subsp. citrulli in watermelon seed ranged from 10 to 106 cfu per seed, but a high
percentage (> 95%) of the infected seed contained relative low cell densities. It was concluded that there is a risk
for an over estimation of the mean populations of the pathogen and thus potentially an under estimation of the seed
sample size required for a desired level of confidence.
 25

4. Xcc in organic seed production

At a workshop held at Ryton Organic Gardens (Ryton, UK, 1 November, 2001), it was black rot was listed as one of
the two important seedborne pathogens in organic cabbage production. Further, no specific literature on the risks
for Xcc infections in organic seed production is known, but overall an increased risk on seed infections can be
expected. It can be hypothesized that the use of organic manures in stead of fertilizers reduces the risk on
oversized succulent transplants, which result in extended periods of leave wetness and which are more susceptible
for Xcc infections (Williams, 1980). On the other hand, it was found that high levels of nitrogen slowed down the
colonization of cabbage plants by Xcc (McElhaney et al., 1998).

High microbial activity in soils may favor rapid decomposting of crucifer residues which reduces risks for
introduction of Xcc in organic seed production compared to conventional systems. The lack of efficient means for
weed control, however will increase the risks for survival of Xcc in the absence of Brassica’s.

Increased risks for damage by pests and diseases due to the avoidance of chemical pesticides will enhance risks on
wounding and infection. The relative high number of insects in organic cabbage plants may also increase the risks
for dissemination of Xcc and flower infections.
26
 27

5. Literature

Remark. Literature was collected from the following sources: Biological Abstracts, Current Contents, CABI abstracts,
IBL kennisbank, the FiBL data base, and literature collections from the former RPVZ (Wageningen, the Netherlands).

Alvarez, A.M., A.A. Benedict, C.Y. Mizumoto, J.E. Hunter & D.W. Gabriel, 1994.
Serological, pathological, and genetic diversity among strains of Xanthomonas campestris infecting crucifers.
Phytopathology 84: 1449-1457.
Arias, R.S., G. Mochizuki, R. Fukui & A.M. Alvarez, 1996.
Most probable number method to enumerate a bioluminescent Xanthomonas campestris pv. campestris in soil.
Soil Biology and Biochemistry 28: 1725-1728.
Clayton, E.E., 1925.
Second progress report of black rot (Pseudomonas campestris) investigations on Long Island; seed infection
and seasonal development. Phytopathology 15: 48-49.
Cook, A.A., J.C. Walker & R.H. Larson, 1952a.
Studies on the disease cylcle of black rot of crucifers. Phytopathology 42: 162-167.
Cook, A.A., R.H. Larson & J.C. Walker, 1952b.
Relation of the black rot pathogen to cabbage seed. Phytopathology 42, 316-320.
Dane, F. & J.J. Shaw, 1993.
Growth of bioluminescent Xanthomonas campestris pv. campestris in susceptible and resistant host plants.
Molecular Plant Microbe Interactions 6: 786-789.
Dane, F. & J.J. Shaw, 1996.
Survival and persistence of bioluminescent Xanthomonas campestris pv. campestris on host and non-host
plants in the field environment. Journal of Applied Bacteriology 80: 73-80.
Dedej, S., K.S. Delaplane & H. Scherm, 2004.
Effectiveness of honey bees in delivering the biocontrol agent Bacillus subtilis to blueberry flowers to suppress
mummy berry disease. Biological Control 31: 422-427.
Dzhalilov, F.S. & R.D. Tiwari, 1995.
Soil and cabbage plant debris as infection sources of black rot. Archives of Phytopathology and Plant
Protection 29: 383-386.
Ercolani, G.L., 1970.
A study of the individual susceptibility of pear flowers to Pseudomonas syringae. Phytopathologia Mediterranea
9: 35-38.
Gitaitis, R.D., R.R. Walcott, F.H. Sanders & C.C. Block, 2005.
A lognormal distribution of phytopathogenic bacteria in seed health assays. In: Abstracts of the 5th ISTA-SHC
Seed Health Symposium, 10-13 May 2005, Angers. France. Abstract nr. 32.
Kamoun, S. & C.I. Kado, 1990.
Phenotypic switching affecting chemotaxis, xanthan production and virulence in Xanthomonas campestris.
Applied and Environmental Microbiology 56, 3855-3860.
Kocks, C.G., M.A. Ruissen, J.C. Zadoks & M.G. Duijkers, 1998.
Survival and extinction of Xanthomonas campestris pv. campestris in soil. European Journal of Plant Pathology
104: 911-923
Kuan, T.L., G.V. Minsavage & N.W. Schaad, 1986.
Aerial dispersal of Xanthomonas campestris pv. campestris from naturally infected Brassica campestris.
Plant Disease 70: 409-413
McElhaney, R., A.M. Alvarez & C.I. Kado, 1998.
Nitrogen limits Xanthomonas campestris pv. campestris invasion of the host xylem. Physiological and
Molecular Plant Pathology 52: 15-24
Morita, A., 1995.
Occurrence of bacterial blossom blight of kiwifruit and its influence on fruit production in Nagasaki Prefecture.
Annals of the Phytopathological Society of Japan 61: 57-62.
28

Neergaard, P. 1979.
Seed Pathology, Volume 1. page 589-590. The MacMillan Press LTD, London and Basingstoke. 839 pages.
Poplawsky, A.R. & W. Chun, 1998.
Xanthomonas campestris pv. campestris requires a functional pigB for epiphytic survival and host infection.
Molecular Plant Microbe Interactions 11: 466-475.
Roberts, S.J., L.H. Hiltunen, P.J. Hunter & J. Brough, 1999.
Transmission from seed to seedling and secondary spread of Xanthomonas campestris pv. campestris in
Brassica transplants: effects of dose and watering regime. European Journal of Plant Pathology 105: 879-889.
Rudolph, K.W.E., M. Gross, F. Ebrahim-Nesbat, M. Nöllenburg, A. Zomorodian, K. Wydra, M. Neugebauer,
U. Hettwer, W. El-Shouny, B. Sonnenberg & Z. Klement, 1994.
The role of extracellular polysaccharides as virulence factors for phytopathogenic pseudomonads and
xanthomonads. In: Kado, C.I. and Crosa, J.H. (eds). Molecular mechanisms of bacterial virulence. pp. 357-378.
Kluwer, Dordrecht, the Netherlands.
Schaad, N.W. & J.C. Dianese, 1981.
Cruciferous weeds as sources of inoculum of Xanthomonas campestris in black rot of crucifers.
Phytopathology 71: 1215-1220.
Schaad, N.W., W.R. Sitterly & H. Humaydan, 1980.
Relationship of incidence of seedborne Xanthomonas campestris to black rot of crucifers. Plant Disease
formerly Plant Disease Reporter 64: 91-92.
Schaad, N.W. & W.C. White, 1974.
Survival of Xanthomonas campestris in soil. Phytopathology 64: 1518-1520.
Shelton, A.M. & J.E. Hunter, 1985.
Evaluation of the potential of the flea beetle Phyllotreta cruciferae to transmit Xanthomonas campestris pv.
campestris, causal agent of black rot of crucifers. Canadian Journal of Plant Pathology 7: 308-310.
Shigaki, T., S.C. Nelson & A.M. Alvarez, 2000.
Symptomless spread of blight-inducing strains of Xanthomonas campestris pv. campestris on cabbage
seedlings in misted seedbeds. European Journal of Plant Pathology 106: 339-346.
Van der Zwet, T., B.G. Zoller, S.V. Thomson, 1988.
Controlling fire blight on pear and appel by accurate prediction of the blossom blight Phase. Plant Disease
72, 464465.
Walcott, R.R., A. Fessehaie, J.T. Lessl & A.C. Castro, 2005.
The role of blossoms in watermelon seed infestation by Acidovorax avenae subsp. citrulli, causal agent of
bacterial fruit blotch. In: Abstracts of the 5th ISTA-SHC Seed Health Symposium, 10-13 May 2005,
Angers. France. Abstract nr. 24.
Williams, P.H., 1980.
Black rot: a continuing threat to world crucifers. Plant Disease 64: 736-742.