MASTERING ELECTROPHYSIOLOGY
IN FREE-MOVING ANIMALS
Equipment
Equipment for
for Biomedical
Biomedical Research
Research
Animal
Animal Behavior
Behavior &
& Electrophysiology
Electrophysiology
www.evolocus.com
Neurologger ®
records behavior and
electrophysiological data
For laboratory
and field studies
• EEG/LFPs up to 256 channels
• Neurons up to 64 channels
• ECG
• Vocalization up to 100 kHz
• Activity
• Temperature
• LFPs
• Heart rate & SpO2
• EMG
• EOG
• Expandable by optical or
electrical brain stimulation,
motorized microdrive, GPS Fits to mice and larger animals
New 3rd
generation!
Verified:
70+
publications!
Neurologger ®
The second generation - for mice and larger animals
Neurologger 2A
Infrared (IR) receiver
for synchronization 4 neurophysiological channels with sampling rate up to 19.2 kHz
for EEG, ECG, EOG, EMG, LFPs and neuronal activity.
3-D accelerometer 3-D accelerometer
Infrared (IR) synchronization with external equipment.
The recorder weight in the most complete configuration is 1.71 g
Connector for + ~1 g typical battery weight makes total weight 2.71 g.
electrophysiological The battery holder can add 0.63 g.
signals
Neurologger 2A other variants
Only The lightest version: The same as before Micro board with
3-D accelerometer, electrodes are soldered but with the connector 3-D accelerometer
256 MB memory, directly to the board, for the electrodes, is added, weight of
weight 1.28 g clip connector for data weight 1.05 g recording combination
downloading, 1 GB, 1.61 g
weight 0.95 g
Water-proof ECG recorder for marine birds
IR receiver is replaced by magnetic sensor.
Putting permanent magnet in the vicinity
of the sensor creates a label in the logger record.
Memory 1 GB, rechargeable battery 3.7V, 240 mAh.
The battery is sufficient for 4 days.
Total weight of encapsulated logger 20 g.
Neurologger 2B
Enhanced version with increased data rates
4 channels with sampling rate up to 33.3 kHz for sound and neuronal activity recording.
Single-channel 200 kHz mode for ultrasound recording.
Microphone IR receiver
Microphone
Contact
microphone
Version for zebra finches, marmosets Version for bats
Neurologger 2A/2B options complementing brain activity recording
4-channel optogenetic stimulator Enhanced accelerometer board Pulse oximeter
Activates or suppress up to 16-bit 3-D accelerometer, Records heart rate and
4 brain areas independently 3-D gyroscope, 3-D magnetic blood oxygenation (SpO2)
and wirelessly compass, altimeter, thermometer non-invasively
The third generation - multichannel neuronal recording in mice, rats, etc.
Bluetooth for configuring Ultrasonic (<100 kHz)
and data access microphone
3-D accelerometer, 3-D gyroscope,
Expansion connector IR receiver for 3-D magnetic compass for locomotion recording.
for optical or electrical precise Weight of depicted combination
brain stimulation, synchronization with 32-channel Omnetics connector 1.96 g.
motorized microdrive,
It can collect 1.25 h of neuronal data being
GPS Micro SDXC powered from 1.05 g battery.
32 neuronal chanels, data storage
sampling rate
up to 20.8 kHz Available neuronal headstages
Ultrasonic recorder with 3-D accelerometer, 16-channel 32-channel 16-channel 32-channel
3-D gyroscope, 3-D magnetic sensor. Hirose Hirose Omnetics Omnetics
Weight 1.29 g 0.35 g 0.44 g 0.46 g 0.67 g
Scalable architecture: from 16 to 256 channels
Neurologger 3
64 channels Neurologger 3, 256 channels
Neurologger Synchronizer
The tool for precise synchronization of Neurologgers of all generations with external equipment
Possible synchronization sources
Analog input with adjustable threshold for
synchronization by a pattern of TTL or audio pulses
IR emitter IR emitter
USB interface
seen as a serial
communication
port in software
Analog output for optional monitoring or
recording of synchronizing events
Standard serial interface is supported in Matlab, Labview, Python and all classical programming languages.
We provide Matlab examples illustrating interaction with Neurologger Synchronizer. Analog input with
adjustable threshold also can be used with a push button. Pressing on this button will initiate transmission
of a predefined pattern of IR pulses to the Neurologgers. Software for conversion of Neurologger data to
Matlab and other environments is provided.
Comparison of three generations of Neurologgers®
Neurologger 1 Neurologger 2A/2B Neurologger 3
Primary usage EEG, EMG and LFPs EEG, EMG and LFPs recording in mice Multichannel neuronal recording in mice and
recording in large and larger animals; ECG, vocalization larger animals; vocalization recording including
animals: marine recording including ultrasonic ultrasonic
mammals, ruminants
Number of channels 8 differential channels 4 channels freely assigned to two 16, 32, 64, 96, 128, 192, 256 channels with one
referent electrodes; from them one referent electrode; or 16-128 differential
can be used for temperature recording channels; up to 3 channels for temperature
ADC resolution 10 bit; 2x oversampling in 10 bit; 2x-8x oversampling in low- 16 bit
low-frequency modes frequency modes
Sampling rate 8 channels up to 800 Hz; Version 2A: Number of channels: Maximal rate
or 2 channels up to 10 4 channels up to 20 kHz 16: 40 kHz; 32: 20.8 kHz; 64: 10.4 kHz; 96: ~7 kHz
kHz; or 1 channel 20 kHz; 128: 5.2 kHz; 192: ~3.5 kHz; 256: ~1.5 kHz
higher sampling rates by Version 2B:
request 4 channels up to 33.3 kHz; or 1 channel Starting from 32 channels: free selection of
up to 200 kHz channel sequence; selected channels can be
sampled more often than others
Locomotion recording Optional analog 3-D Optional 3-D accelerometer; optional 3-D accelerometer, 3-D gyroscope, 3-D magnetic
accelerometer occupies 3-D accelerometer, 3-D gyroscope, 3-D compass
three channels magnetic compass, altimeter
Vocalization recording - Optional microphone and contact Dedicated 12-bit 200 ksps microphone ADC
microphone are connected to works simultaneously with neuronal 16-bit ADC;
neurophysiological channels; optional optional dynamic range expansion; attachment
dynamic range expansion of a microphone to one 16-bit channel is also
possible
Expansion possibilities Asynchronous serial bus Dedicated communication controller Inter-integrated circuit (I2C) communication bus
up to 1.5 Mbps (UART) with different peripheral interfaces is 400 kbps makes possible chained connection of
and digital input/output connected to 8 Mbps synchronous bus multiple custom-developed peripheral devices.
lines at the main CPU can (SPI). It can be custom programmed by Development of the following peripheral units is
be custom programmed request. Current expansions include: 4- planned or will be done by request: optical and
by request. channel optogenetic stimulator, heart electrical brain stimulators, motorized
rate and blood oxygenation (SpO2) microdrive, GPS. 32-ch version has 3 auxiliary
sensor. analog inputs and one digital output.
Data memory Micro SD high-capacity Soldered memory chip 1-2 GB Micro SD high-capacity (4-32 GB) or extended
(4-32 GB) memory card capacity (64-256 GB, Micro SDXC) memory card
Maximal memory filling 30 kBps (2 channels, 10 300 kBps (1 channel, 200 kHz) 1.77MBps (32 channels 20.8 kHz, sound 125 kHz,
speed kHz) motion sensors 580 Hz)
Maximal recording duration Limited by the battery 1 GB will be filled when 4 channels are In most cases limited by the battery. 128 GB card
sampled with the frequency: is sufficient for recording during 20 hours in the
100 Hz: 20 days 17 h highest data rate mode listed above. If only 32
400 Hz: 5 days 4 h channels are sampled with the frequency 15.625
1600 Hz: 1 day 7 h kHz, 128 GB is sufficient for 31 hours.
9.6 kHz: 5 h 10 min
19.2 kHz: 2 h 35 min
33.3 kHz: 1 h 29 min
One channel at 200 kHz: 59 min 39 s
3D accelerometer increases volume by
50% in low-frequency modes.
Current consumption ~5.5 mA in EEG mode Version 2A: 1.5 - 4.3 mA, mode- 11-25 mA, mode-dependent;
dependent; In EEG mode with 3D All neuronal modes ~25 mA.
accelerometer. 2.0 mA
Version 2B: in high-frequency modes
( ≥ 3 3 . 3 k H z ) : 6 . 0 - 1 1 . 7 m A , m o d e -
d e p e n d e n t
Logger size (w/o battery) 36x31x6 mm From 18x15x3 mm to 22x15x8 mm From 20x15x6 mm to 24x15x8 mm
Logger weight (w/o battery) 5.31 g 0.95 - 1.71 g, version-dependent 1.29 – 1.96 g, version-dependent
Recommended batteries Lithium-polymeric 3.7V A couple of non-rechargeable Zn-Air Lithium-polymeric 3,7V
and their weights 240 mAh 9.0 g 1.4V batteries ZA 10 (75 mAh), ZA312 20 mAh, 0.63 g
rechargeable battery will (175 mAh), ZA13 (305 mAh) with the 40 mAh, 1.05 g
be sufficient for 1 day weights per pair 0.635, 1.02, 1.66 g 50 mAh, 1.58 g
19h. Non-rechargeable respectively will be sufficient for 1, 2 Will provide duration of neuronal recording of 15
3.6V 1200 mAh 8.9 g and 4 days of EEG recording. Lithium- min, 1 h 15 min and 2 h 15 min respectively.
LS14250 will be sufficient polymeric batteries, for instance 3.7V
for 9 days. 12 mAh 0.38 g GM300910, also can be
used.
Evolocus LLC
Evolocus
Equipment for Biomedical Research
177 White Plains Road, 42B
Tarrytown, NY 10591, USA
Tel.: +1 (914) 329-0035
Neurologger is a registered trademark. Fax: +1 (914) 631-3421
Protected by U.S. patents #8,160,688; Animal Behavior & Electrophysiology
#9,492,085. Other patents pending. www.evolocus.com E-mail: [email protected]
