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Investigating The Rate of Transpiration Using A Potometer

The document outlines an experiment to investigate the rate of transpiration using a potometer, detailing the necessary apparatus and method. It emphasizes the importance of airtight connections and describes how to set up the apparatus, introduce an air bubble, and measure its movement over time. Additionally, it suggests varying environmental conditions such as light, wind, humidity, and temperature to observe their effects on transpiration rates.

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37 views1 page

Investigating The Rate of Transpiration Using A Potometer

The document outlines an experiment to investigate the rate of transpiration using a potometer, detailing the necessary apparatus and method. It emphasizes the importance of airtight connections and describes how to set up the apparatus, introduce an air bubble, and measure its movement over time. Additionally, it suggests varying environmental conditions such as light, wind, humidity, and temperature to observe their effects on transpiration rates.

Uploaded by

adam.memi7
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as ODT, PDF, TXT or read online on Scribd
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Investigating the Rate of Transpiration Using a Potometer

Apparatus:

Cut shoot: A leafy shoot freshly cut underwater.

 Rubber bung: Ensures airtight connection.

Reservoir: Allows the air bubble to be reset for repeated trials.

Capillary tube with scale: Measures the movement of the air bubble.

Air bubble: Used as a marker to track water uptake.

Beaker of water: Maintains hydration and connects to the capillary tube.

Method:
Set up the apparatus as shown in the diagram. Ensure all joints are airtight using Vaseline or similar sealant.

Fill the entire potometer with water (including the capillary tube).

Cut the shoot underwater at a slant to increase surface area and prevent air entering the xylem.

Insert the shoot into the rubber bung securely.

Introduce a single air bubble into the capillary tube by briefly lifting the end out of the beaker.

Start timing using a stopwatch and record the position of the air bubble against the volume scale.

Allow the air bubble to move along the scale as the plant transpires.

Record the distance moved over a set time (e.g 5 minutes).

Use the reservoir tap to flush the system and reset the air bubble.

Repeat under different environmental conditions:

 Increase light with a lamp

 Increase wind using a fan

 Increase humidity using a plastic bag and damp paper towel

 Raise temperature gently with a heater

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