complex Acinetobacter baumannii Acinetobacter calcoaceticus Acinetobacter nosocomialis Acinetobacter pitti

oris Aeromonas sp Aeromonas allosaccharophila Aeromonas caviae Aeromonas culicicola Aeromonas hydrop
ichthiosmia Aeromonas veronii Aeromonas sobria Alcaligenes faecalis Bacteroides_ovatus group Burkholderia
kholderia multivorans Burkholderia cepacia complex Burkholderia ambifaria Burkholderia anthina Burkholderia c
Burkholderia cepacia Burkholderia diffusa Burkholderia dolosa Burkholderia lata Burkholderia latens Burkholder
Burkholderia pyrrocinia Burkholderia seminalis Burkholderia stabilis Burkholderia vietnamiensis Candida dublini
glabrata Citrobacter amalonaticus complex Citrobacter amalonaticus Citrobacter farmeri Citrobacter freundii com
braakii Citrobacter freundii Citrobacter gillenii Citrobacter murliniae Citrobacter werkmanii Citrobacter youngae C
dentium Citrobacter sedlakii Citrobacter koseri Corynebacterium bovis Corynebacterium amycolatum Delftia_a
Eikenella corrodens Enterobacter aerogenes Enterobacter cloacae complex Enterobacter asburiae Enterobacte
Enterobacter cloacae Enterobacter hormaechei Enterobacter kobei Enterobacter ludwigii Enterococcus gallinar
coli Finegoldia magna Geotrichum capitatum Haemophilus haemolyticus Haemophilus influenzae Haemophilu
Hafnia alvei Klebsiella pneumoniae Klebsiella_oxytoca / Raoultella_ornithinolytica Kingella kingae Kocuria kristin
la_sg_Branhamella catarrhalis Moraxella_sg_Moraxella osloensis Morganella morganii Pantoea agglomerans P
distasonis Parabacteroides distasonis Pasteurella multocida Pediococcus pentosaceus Propionibacterium acne
bilis Proteus vulgaris group Proteus hauseri Proteus penneri Proteus vulgaris Providencia rettgeri Providencia st
monas aeruginosa Pseudomonas fluorescens group Pseudomonas oryzihabitans Pseudomonas azotoformans
brenneri Pseudomonas cedrina Pseudomonas congelans Pseudomonas corrugata Pseudomonas extremorient
nas fluorescens Pseudomonas gessardii Pseudomonas libanensis Pseudomonas mandelii Pseudomonas marg
monas migulae Pseudomonas mucidolens Pseudomonas orientalis Pseudomonas poae Pseudomonas rhodes
nas synxantha Pseudomonas tolaasii Pseudomonas trivialis Pseudomonas veronii Pseudomonas putida group
fulva Pseudomonas monteilii Pseudomonas mosselii Pseudomonas plecoglossicida Pseudomonas putida Roth
Rothia mucilaginosa Salmonella sp Salmonella enterica ssp arizonae Salmonella enterica ssp bongori Salmone
diarizonae Salmonella enterica ssp enterica Salmonella enterica ssp houtenae Salmonella enterica ssp indica S
terica ssp salamae Serratia rubidaea Serratia liquefaciens Serratia marcescens Stenotrophomonas maltophilia S
agalactiae Streptococcus anginosus Streptococcus constellatus Streptococcus dysgalactiae Streptococcus gal
coccus gordonii Trichosporon asahii Vibrio parahaemolyticus Vibrio vulnificus Achromobacter xylosoxidans Acin
Acinetobacter radioresistens Acinetobacter ursingii Acinetobacter baumannii complex Acinetobacter baumann
calcoaceticus Acinetobacter nosocomialis Acinetobacter pittii Actinomyces oris Aeromonas sp Aeromonas all
Aeromonas caviae Aeromonas culicicola Aeromonas hydrophila Aeromonas ichthiosmia Aeromonas veronii Ae
Alcaligenes faecalis Bacteroides_ovatus group Burkholderia gladioli Burkholderia multivorans Burkholderia cepa
kholderia ambifaria Burkholderia anthina Burkholderia cenocepacia Burkholderia cepacia Burkholderia diffusa B
Burkholderia lata Burkholderia latens Burkholderia metallica Burkholderia pyrrocinia Burkholderia seminalis Burk
Burkholderia vietnamiensis Candida dubliniensis Candida glabrata Citrobacter amalonaticus complex Citrobacte
Citrobacter farmeri Citrobacter freundii complex Citrobacter braakii Citrobacter freundii Citrobacter gillenii Citrob
Citrobacter werkmanii Citrobacter youngae Citrobacter rodentium Citrobacter sedlakii Citrobacter koseri Coryne
Corynebacterium amycolatum Delftia_acidovorans group Eikenella corrodens Enterobacter aerogenes Enterob
complex Enterobacter asburiae Enterobacter cancerogenus Enterobacter cloacae Enterobacter hormaechei En
Enterobacter ludwigii Enterococcus gallinarum Escherichia coli Finegoldia magna Geotrichum capitatum Haem
icus Haemophilus influenzae Haemophilus parainfluenzae Hafnia alvei Klebsiella pneumoniae Klebsiella_oxytoc
nithinolytica Kingella kingae Kocuria kristinae Moraxella_sg_Branhamella catarrhalis Moraxella_sg_Moraxella os
ella morganii Pantoea agglomerans Parabacteroides distasonis Parabacteroides distasonis Pasteurella multocid
pentosaceus Propionibacterium acnes Proteus mirabilis Proteus vulgaris group Proteus hauseri Proteus penner
Providencia rettgeri Providencia stuartii Pseudomonas aeruginosa Pseudomonas fluorescens group Pseudomo
Pseudomonas azotoformans Pseudomonas brenneri Pseudomonas cedrina Pseudomonas congelans Pseudom

Changing Microbiology

The MALDI Biotyper® System

Microbial identification with
unequaled speed and accuracy
Innovation with Integrity

RUO/GP
 In microbiology,
speed and
accuracy matter

A powerful technology Identifying microorganisms

for better results by their molecular fingerprint

To help answer key challenges in microbiology, Bruker has utilized The MALDI Biotyper System identifies micro-
its many years of experience to create the truly groundbreaking organisms using MALDI-TOF (Matrix-Assisted
MALDI Biotyper System (MBT). In the past decade, this revolutionary Laser Desorption / Ionization Time-of-Flight)
technology has been adopted in many microbiology laboratories mass spectrometry to determine the unique
worldwide, for reliable, fast and efficient identification of a wide range proteomic fingerprint of an organism. Specif-
of gram-negative and gram-positive bacteria, yeasts and molds, by an ically, the MALDI Biotyper System measures
easy to operate, yet powerful benchtop system. highly abundant proteins that are found in all
microorganisms, and takes advantage of their
Accuracy comparable to Nucleic Acid Sequencing specific differences.
Much faster than traditional methods
Cost-effective The characteristic patterns of these proteins
Robust and easy to use give the unique fingerprint of an organism and
A true benchtop system are used to reliably and accurately identify
Easy to implement a particular microorganism by matching the
Optional workflow improvement tools respective pattern with an extensive reference
library.

The outstanding capabilities of the system go

well beyond microbial identification and Bruker
is continuously working on further innovations.

2
A simple procedure
for a sophisticated and fast platform

Bacteria, yeast or mold:

one workflow for all 01

1 isolate ~20 seconds, 95 isolates + 1 QC sample < 20 min

The MALDI Biotyper System workflow has
been designed to be efficient and easy. No
previous experience with mass spectrome-
try is required. As shown, the fully traceable Add target plate to a MALDI Biotyper project list
workflow has been streamlined and requires
only a few simple steps to generate high qual- 02
ity microorganism identifications. Typically,
no more than an isolated single colony from a
culture plate is required. The hands-on time
per isolate is only 20 seconds for 95% of
the microorganisms. Select an isolated colony

Hands-on time:
Our dedicated microbiology software 03
automates the process of acquiring the
mass spectrum and performing the match
against the extensive reference library.
The identification results, presented using
a ‘traffic light’ color scheme, are effortless
Transfer sample onto the target plate and add matrix
to interpret.

The MALDI Biotyper simplifies microbial 04

identification, and facilitates and harmonizes
the workflow with only one system.

95 isolates + 1 QC sample ~5 min

Faster than ever
MALDI-TOF spectrum
automatically generated by the software
The new MBT Compass HT software
dramatically shortens the time-to-result;
analysis of 95 isolates and 1 QC sample
LIBRARY
05
results in a complete identification report
within ~5 minutes.

Spectrum instantly matched against the

reference library to give identification
Sample preparation hands-on time:
1 isolate ~20 seconds
06
95 isolates < 20 min
Final review and
System analysis time to ID result:
95 isolates + 1 QC sample ~ 5 min
result validation

3
 Easy-to-use software
dedicated to microbiology
In just a few steps, the simple-to-use software guides users through the set-up of samples for analysis.
The MALDI Biotyper System is automatically checked using Bacterial Test Standard (BTS) before each use.
When the check is successful, the system automatically begins the measurement process of the samples.

Range Interpretation

2.00 - 3.00 High Confidence Identification

Instantly after the acquisition of the spectral data of the first sample,
1.70 - 1.99 Low Confidence Identification
the identification results pop up consecutively. The results are clearly listed
0.00 - 1.69 No Organism Identification Possible
under ‘Detected Species’ accompanied by the resulting Log(score) and
appropriate ‘traffic light’ color. When the entire sample run has been com-
pleted, a report is generated.

Easy reviewing and result validation Open microbiology concept - easy

implementation in your laboratory

The informative MALDI Biotyper report facilitates easy validation The MALDI Biotyper allows for smooth
by the microbiologist. Subsequently, a simple click in the software integration with existing AST systems,
enables the export of the MALDI Biotyper results in a LIS or AST laboratory automation systems and laboratory
system compatible form. informatics.

4
More than a comprehensive library

Candida auris

The main spectra concept capturing x104

Intens. [a.u.]
true biological variability
4

Reference library entries in the MALDI

Biotyper system are stored as Main Spectra
(MSP). These MSPs are based on multiple 3
measurements of a single defined strain to
ensure that the true biological variability of an
organism is reflected in the library.
2

Unknowns are then compared to the MSP

library using a superior pattern-matching
approach. This includes peak positions and 1

intensities, ensuring the highest possible

levels of accuracy and reproducibility across
the complete range of microorganisms. 0

0 4000 6000 8000 10000 12000 14000 16000

m/z

A continuously updated reference library High confidence mycobacteria

covering over 4,000 species identification
Bruker is fully committed to the steady development of the reference The optional MBT HT Mycobacteria Module
library. An active program of reference spectra generation culminates for the MALDI Biotyper is the comprehensive
in regular library updates for MALDI Biotyper users. solution for highly reliable and fast myco-
These updates are focused on recommendations from our bacteria identification. It is composed of a
collaboration partners from the industrial, veterinary and clinical software module and a specific reference
field, including certified strain collections. spectrum library covering the majority of the
currently known mycobacteria species.
Taxonomy becomes easy
Create your own libraries and run
The metadata of the MALDI Biotyper Reference Library facilitate
your data comparison
the access to taxonomical information, such as synonyms and
taxonomical modifications. Laboratories that need to create their own
libraries can make use of software tools to
Reduce the need for mycology expertise easily compile customized reference library
entries which can be exported and shared.
The MALDI Biotyper is perceived as the most promising For further investigations, software tools –
alternative for the identification of molds. A dedicated MBT HT such as dendrogram analysis – are available.
Filamentous Fungi Module, including a software module and a
specific reference spectrum library, is available to facilitate the
identification of this challenging group of microorganisms.

5
 The best technology from
the experts in mass spectrometry

A platform suited to your needs Resolution meets sensitivity

Being the leader in MALDI-TOF technology, it is of great importance Resolution and sensitivity are tailored to
to Bruker to design robust, compact, high performance platforms the needs of microbiologists. Due to Bruker’s
intended for extensive and routine usage in the microbiology labora- patented PAN™ resolution the MALDI
tory. Continuous hardware development has led to the 4th generation Biotyper achieves optimal results from a
of Bruker‘s benchtop MALDI Biotyper systems. compact benchtop instrument.

Bruker offers laboratories the opportunity to choose the MALDI-TOF Highly reproducible results
mass spectrometer that best fits their needs:
The quick and simple Bacterial Test Standard
The new MALDI Biotyper sirius one RUO or GP System with quality check, performed before each run,
Bruker’s proprietary lifetime* smartbeam™ solid state laser ensures the highest standard of run-to-run
technology at 200 Hz repetition rate, and positive ion mode. reproducibility.
System improvements, including the newest electronics and a high
performance vacuum system, generate fast target exchange times Continuous operation
for accelerated time-to-result - even faster than before.
The MALDI Biotyper sirius RUO or GP System, with the same The integrated ion source cleaning permits
innovative improvements, smartbeam™ 200 Hz laser and positive as continuous high performance with minimized
well as negative ion detection. The additional capability of analysis in maintenance requirements. Cleaning the
negative ion mode broadens the research applications, such as the source using the separate IR-laser is perfor-
analysis of lipids for e.g. resistance detection. med easily by a few clicks in the software,
without breaking vacuum.

Even shorter Time-to-Result

With Smart Spectra Acquisition™, data generation is accelerated

by minimizing the number of laser shots per sample needed to
acquire a meaningful spectrum. An additional benefit of this function
is the optimal exploitation of the laser lifetime.
The Time-to-Result is further shortened dramatically by the power
of the new MBT Compass HT software, resulting in identification
results popping up instantly after spectra acquisition, one by one,
without delay.
An entirely filled MBT Biotarget 96, holding 95 isolates and 1 QC
sample, results in a complete identification report in ~5 minutes.

6
Compact benchtop systems –
no performance compromise
Optimal performance secured by zero-button IDealTune

Machine Learning (ML) based approaches are opening the door for mining deeper information from MALDI-TOF
spectra, which is also of great interest for antimicrobial resistance prediction. For this kind of promising applications,
it is of utmost importance that the MALDI-TOF system always operates in optimal conditions, to deliver
standardized mass spectra of the highest quality. As Bruker endeavours providing high-level future-proof solutions,
the automated zero-button IDealTune procedure has been developed, fine-tuning the detector, baseline settings and
laser power, without any user intervention.
IDealTune is performed systematically in the background; forget about tedious preparation of dedicated tuning
samples, forget about time-consuming manual tuning.

MALDI Biotyper sirius one RUO System MALDI Biotyper sirius RUO System
MALDI Biotyper sirius one GP System MALDI Biotyper sirius GP System

Speed of 95 isolates + 1 QC sample ~ 5 min to identification

analysis Identification of 600 samples/hr
Identification results popping up instantly after spectra acquisition,
one by one, without delay

Laser Bruker’s proprietary lifetime* smartbeam laser

200 Hz repetition rate
At least 500 million laser shots are guaranteed

Polarity Positive ion mode only Positive and negative ion mode

Mass range 0-500.000 Da; with MALDI Biotyper applications focused to:
0-1.000 Da (resistance detection)
2.000-20.000 Da (microorganism identification)

Vacuum Oil-free membrane pre-vacuum pump and high capacity turbomolecular pump
system high pumping capacity, which in combination with a clever source design results
in very fast target exchange
minimal downtime after maintenance

LED strip to remotely observe system status

Perpetual Ion Source™ with IR-laser self-cleaning functionality
Other
Whispermode™ <60 dB under normal operating conditions
features
Patented PAN™ technology for high mass resolution over a wide mass range
Voltage: 220 V

L x W x H: 500 x 710 x 1070 mm / 19.7 x 28.0 x 42.2”

Dimensions & Net weight: 75 kg / 165.4 lb
Operating Noise: < 60 dB
Parameters Temp Range: 16 - 30°C / 61 - 86°F
Operating Humidity: 20 - 75%, non-condensing

* Lifetime means: 500 million laser shots or seven years (whichever occurs first)

7
 MALDI Biotyper System overview

Benchtop MALDI-TOF system

MALDI Biotyper sirius one RUO System / MALDI Biotyper sirius one GP System,
with 200 Hz smartbeam™ laser and positive ion mode

or
MALDI Biotyper sirius RUO System / MALDI Biotyper sirius GP System,
with 200 Hz smartbeam™ laser and positive and negative ion detection

Routine identification of Mycobacteria identification Filamentous fungi

gram +/- bacteria, yeasts (optional) identification (optional)

Software Software MBT HT Filamentous Fungi

MBT Compass HT software MBT HT Mycobacteria Module Module
MBT Compass Library Consumables
Security Related Library for MBT Mycobacteria Kit
identification of highly
pathogenic microorganisms
(optional)
Identification directly from positive blood cultures
Consumables (optional)
Matrix HCCA-portioned
Bacterial Test Standard Software
MBT Biotarget 96 MBT HT Sepsityper Module
Consumables
MALDI Sepsityper® Kit 50

Resistance detection Accessories for workflow optimization & automation

(optional) (optional)

Software MBT Shuttle ergonomic target holder

MBT HT Subtyping Module MBT FAST™ Shuttle for standardized and accelerated drying of matrix
MBT HT LipidART Module and other liquids
MBT HT STAR®-BL Module MBT Pilot® for guided sample transfer
Consumables MBT Galaxy® for automated application of matrix and formic acid
MBT Lipid Xtract™ Kit
MBT STAR®-Carba Kit
MBT STAR®-Cepha Kit

Please contact your local Bruker sales representative for availability of the optional MBT system components in your country.

8
9
 MBT consumables for basic identification

Bacterial Test Standard (BTS)

The BTS is an E. coli extract spiked with two

high molecular weight proteins and has been
developed for the quality control process
of the MALDI Biotyper System. Its specific
composition covers the entire mass range of
proteins used for precise identification
of microorganisms.

Content: One box consisting of 5 tubes

providing 50 µL per tube / Part No. 8255343

HCCA Matrix, portioned

The instant HCCA matrix enables easy and

convenient preparation of HCCA matrix
solutions. The matrix is soluble in standard
organic solvent, easy to handle, and enables
highly sensitive measurements.

Content: One box consisting of 10 tubes

providing 250 µL per tube / Part No. 8255344

Disposable MBT Biotargets

The ready-to-use disposable MBT Biotargets

offer 96 positions and a unique barcode for
full traceability in paperless workflows.

MBT Biotarget 96
Set of 20 individually barcoded MALDI
Biotyper target plates, 96 positions each /
Part No. 1840375

MSP adapter for MBT Biotarget 96

Adapter required to use MBT Biotargets with
MALDI Biotyper Systems / Part No. 8267615

10
MBT workflow accessories

MBT Shuttle Target Holder

The MBT Shuttle target holder is used to

securely hold MBT Biotargets during the
sample preparation process. The secure grip,
non-slip rubber feet and ergonomic shape
make sample preparation easier.

One target holder / Part No. 1847032

MBT FAST™ Shuttle

Standardized and accelerated drying of

MALDI Biotyper matrix and other liquid
reagents

Part No. 1872847

MBT Pilot®

The MBT Pilot facilitates correct sample

positioning through patented microprojection
technology by indicating the next free MALDI
target plate position.

Part No. 1822041

MBT Galaxy®

The MBT Galaxy, for automated application of

HCCA matrix and formic acid, frees laboratory
personnel from cumbersome pipetting while
ensuring the highest preparation quality
under controlled conditions and complete
traceability in a paperless workflow.

Part No. 1821269

11
 No. 1800236 © 09-2022 Bruker Daltonics

Not for use in clinical diagnostic procedures.

Please contact your local representative for availability in your country.

MALDI Biotyper ®, MALDI Sepsityper ®, MBT Galaxy ®, MBT Pilot ® and MBT STAR® Online information
are registered trademarks of the Bruker group of companies. bruker.com/microbiology

Bruker Daltonics GmbH & Co. KG Bruker Scientific LLC

Bremen · Germany Billerica, MA · USA
Phone +49 (0) 421-2205-0 Phone +1 (978) 663-3660

[email protected]