BD FACSLyric Flow Cytometry TM
System Technical Specifications
The BD FACSLyricTM System includes The software that controls the
the BD FACSLyricTM Flow Cytometer, BD FACSLyricTM Flow Cytometry System
the optional BD FACSTM Universal is comprised of two applications: BD FACSLyric
Loader and workstation that runs the • The BD FACSuiteTM Clinical Application
software. All these components supporting BD CE-IVD Assays with
combine to create an integrated assay templates:
system with a compact footprint.
BD TritestTM CD3/CD4/CD45 Kit
The BD FACSLyric Flow Cytometer is
TM
BD TritesTM CD4/CD8/CD3 Kit with
available in 4, 6, 8, 10 or 12 colors and BD TrucountTM Tubes
equipped with a blue, red and violet BD MultitestTM CD3/CD8/CD45/CD4 Kit
laser depending on the configuration. BD MultitestTM CD3/CD16+CD56/
The BD FACSLyricTM Flow Cytometer is • The BD FACSuiteTM Application
CD45/CD19 Kit
upgradeable up to 12 colors. supporting BD IVD Single Color
BD MultitestTM IMK Kit
Sample acquisition can be manual or Reagents and user-defined panels.
BD MultitestTM 6-Color TBNK Kit
automated via the BD FACSTM Functions within the application
BD® Stem Cell Enumeration Kit
Universal Loader. The Loader provides BD LeucocountTM Kit facilitate instrument-to-instrument and
walkaway operation with samples BD TrucountTM Controls site-to-site standardisation.
loaded in either microtiter plates or 12 BD OneFlowTM LST,
x 75-mm tube racks. B-CLPD T1, PCST, PCD and ALOT
The BD FACSLyricTM Flow Cytometer BD MultitestTM and BD TritestTM CD3/
integrated with the BD FACSDuetTM CD4/CD45 are also available with
Sample Preparation System provides BD TrucountTM Tubes.
consistency and workflow efficiency
support for pre-analytical sample
preparation.
BD
BD FACSLyricTM Flow Cytometry System
Technical Specifications
Optics Software
Available system configurations Sheath fluid consumption • Integrated bi-directional LIS interface
4-color: 2-laser (blue, red) (3-1) Normal: 13.6 mL/min using BD FACSTM Workflow Manager
6-color: 2-laser (blue, red) (4-2) High sensitivity: 6.6 mL/min • Support for 21 CFR Part 11 workflow
8-color: 3-laser (blue, red, violet) (4-2-2) Supported tubes, plates and tube with audit trail and e-signature
10-color: 3-laser (blue, red, violet) (4-3-3) racks • Universal setup for fast and
12-color: 3-laser (blue, red, violet) (4-3-5) convenient instrument setup and
• With BD FACSTM Universal Loader standardization
Solid-state laser specifications Tubes • Single-tube QC with BD® CS&T Beads
Blue laser: 488 nm, 20 mw 30-tube rack (12 x 75-mm tubes) • QC module with Levey-Jennings plots
Red laser: 640 nm, 40 mw 40-tube rack (12 x 75-mm tubes) • Two applications
Violet laser: 405 nm, 40 mw
Plates BD FACSuiteTM Application
Beam spot size (all lasers) 96 FalconTM standard height, round, • User-defined assays
9 μm x 63 μm polystyrene • User-defined plots
Optical alignment 96 FalconTM standard height, • User-defined worksheets and reports
Auto alignment on demand flat, polystyrene • User-defined tube/reference settings
• Expression editing
Flow-cell lens 96 FalconTM standard height, round,
1.2 NA polypropylene BD FACSuiteTM Clinical Application
Pre-configured workflow and pre-set
FSC detector 96 FalconTM standard height, conical, templates for the following BD CE-IVD
Photodiode polypropylene assays:
SSC and FL detectors 384 Greiner standard height, • BD TritestTM
PMT flat, polystyrene • BD MultitestTM 4-Color
See filter guide for optical configurations. • BD MultitestTM 6-Color TBNK
96 FalconTM, half deep, conical,
polypropylene • BD TrucountTM Controls
Fluidics • BD OneFlowTM LST,
Flow cell 96 FalconTM, deep, conical,
B-CLPD T1, PCST, PCD and ALOT
Stainless steel with low coefficient of polypropylene
• BD® Stem Cell Enumeration
thermal expansion for predictable, 96 Milipore, filter bottom, • BD LeucocountTM
stable performance polypropylene
Report in 26 languages
Cuvette internal cross-section • With manual tube port
430 μm x 180 μm FalconTM 5 mL (12 x 75-mm) polystyrene QC
and polypropylene Automated single-tube QC
Sample flow rates
BD TrucountTM Tubes 5 mL (12 x 75 mm) with BD® CS&T Beads
Low: 12 μL/min
Medium: 60 μL/min Falcon 15 mL
High: 120 μL/min Falcon 50 mL
High sensitivity: 50 μL/min Microcentrifuge 2 mL
Fluid capacity Sample dead volume
Standard 5-L tanks 30 μL (12 x 75 mm tubes)
Optional 10-L tanks Cytometer schedule settings
Adapter available for 20-L Pre-programmed startup and idle
BD FACSFlowTM Cubitainer shutdown
Sheath core stream fluid velocity
Normal: 5.4 m/s
High sensitivity: 2.7 m/s
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BD FACSLyricTM Flow Cytometry System
Performance
Acquisition rate
Up to 35,000 events per second. No Parameters Installation requirements
limit on number of events acquired in
Area (A), Width (W), Height (H) for Operating temperature
a single FCS file
all channels and Time (T). Total of 43 15°C (59°F) to 30°C (86°F)
Carryover* parameters available. Maximum of ±2.5°C/day fluctuation
≤0.1% with default SIT flush** 3 scales: recommended
<0.05% with 3 or more SIT flushes Linear (A, W, H) Humidity
with 500 µL of sample.** Logarithmic (A, H) 15% to 85% relative humidity
Biexponential (A, W, H) (noncondensing)
Sensitivity
Compensation Dimensions (W x D x H)
FITC: <85 MESF
Full inter-beam matrix, during or post Cytometer
PE: <20 MESF
acquisition 63.3 x 57.9 x 57.9 cm
Channel Qr (x1,000) 24.9 x 22.8 x 22.8 in.
Threshold
FITC 20 Any single parameter or logical With standard tanks
PE 133 combination of multiple parameters 85.2 x 57.9 x 57.9 cm
PerCP-Cy 5.5 13 33.5 x 22.8 x 22.8 in.
PE-Cy7 17 Data management
APC 10 With standard tanks and loader
Workstation specifications
BD HorizonTM APC-R700 8 107.2 x 57.9 x 57.9 cm
(minimum required)
APC-Cy7 7 42.2 x 22.8 x 22.8 in.
Clock speed of 3.2 GHz
BD HorizonTM V450 47 16 GB RAM Weight
BD HorizonTM V500-C 17 Cytometer: 56.0 kg (123.5 lb)
BD HorizonTM BV605 133 Hard drive and data storage
Loader: 13.2 kg (29 lb)
BD HorizonTM BV711 43 1 TB Solid State HD
BD HorizonTM BV786 16 Power specifications
Operating system
Voltage: 100–240 ±10% VAC
Fluorescence precision MicrosoftTM WindowsTM 10 IoT
Frequency: 50–60 ±10% Hz
<3% CV for chicken erythrocyte 64-bit OS
Current: 2 A
nuclei (CEN) Peripheral devices Power: 200 W
Fluorescence linearity At least 3 USB ports
Operational heat dissipation
2 ±0.05% for CEN HPTM USB Keyboard US
<498 BTU/hour
HPTM USB Optical Mouse
Data resolution Noise under normal operating
Uncompensated data has a range of Networking conditions
0–262,143 <55 dBA over 8 hours under normal
Ethernet LA 10/ 100 /1000
SSC and FSC resolution Signal Processing operating conditions
Enables separation of 0.2-μm 18-bit dynamic range with IEEE Altitude
beads from noise 32 bit floating-point resolution ≥0.8 atm (approximately
System throughput Monitor 2,000 meters)
≤50 minutes for a 40-tube rack with a LCD flat panel, 23 in.
standard BD TritestTM Assay stopping LCD flat panel, 27 in. (recommended)
rule on samples with normal CD4
counts (approximately 1190 cells/μl). Data management options
≤40 minutes for a 96-well plate, using BD FACSTM Workflow Manager for LIS
default mix settings, a two-second connectivity.
acquisition, and a SIT flush BD Remote Support Services for
in between each well and no remote troubleshooting capability
preview before acquiring or report
review delay.
*Carryover may vary based on the application and population of interest. ** Determined for CD45 population using CD45 PerCP.
3
BD FACSLyricTM Flow Cytometry System
Specifications for System Options
BD FACSTM Universal Loader Handheld barcode scanner
Compatible with 30 (barcoded) or 40 Handheld barcode scanner with stand
(non-barcoded) tubes (12 x 75 mm). supporting GS-1 standard 1-D and 2-D formats
Equipped with an orbital shaker for in
Extended-use fluidics
place mixing and resuspension of cells.
Optional tanks and connectors to allow
Optimized for all supported plate
for use with 10-L waste tanks and
and tube formats. Includes internal barcode
BD FACSFlowTM Cubitainers
reader for positive sample identification.
BD FACSDuetTM Sample Preparation System
Supported barcode formats
Integrated with the BD FACSLyricTM Flow Cytometer
Codabar
provides pre-analytical automation.
Code 128
Code 3 of 9
Interleaved 2 of 5
BD FACSLyricTM Flow Cytometers and BD FACSDuetTM Sample Preparation System are Class 1 Laser Products.
BD Tritest™ CD3/CD4/CD45, BD Tritest™ CD3/CD4/CD45 with BD Trucount™ Tubes, BD Tritest™ CD4/CD8/CD3 with BD Trucount™ Tubes, BD
Multitest™ CD3/CD8/CD45/CD4, BD Multitest™ CD3/CD8/CD45/CD4 with BD Trucount™ Tubes, BD Multitest™ CD3/CD16+CD56/CD45/CD19, BD
Multitest™ CD3/CD16+CD56/CD45/CD19 with BD Trucount™ Tubes, BD Multitest™ 6-Color TBNK, BD Multitest™ 6-Color TBNK with BD Trucount™
Tubes, BD Multitest™ IMK Kit, BD Multitest™ IMK Kit with BD Trucount™ Tubes, BD Leucocount™ Kit, BD® Stem Cell Enumeration Kit, BD Trucount™
Controls, BD OneFlow™ LST, BD OneFlow™ B-CLPD T1, BD OneFlow™ PCST, BD OneFlow™ PCD, BD OneFlow™ ALOT are in vitro diagnostic medical
devices bearing a CE mark and are CE certified by BSI Group The Netherlands B.V. (Notified Body Number = 2797).
BD FACSLyric™ Flow Cytometer with the BD FACSuite™ Clinical and BD FACSuite™ applications, BD FACS™ Universal Loader, BD FACSDuet™ Sample
Preparation System, BD FACSuite™ Clinical, BD Tritest™ 3-Color Assays, BD FACSuite™ Clinical BD Multitest™ 6-Color Assays, BD FACSuite™ Clinical
BD Multitest™ 4-Color Assays, BD FACSuite™ Clinical BD Trucount™ Controls, BD OneFlow Assay Installer I, BD OneFlow™ Assays Installer II, BD®
Stem Cell Enumeration Assay Module and BD Leucocount™ Assay Module are in vitro diagnostic medical devices bearing a CE mark.
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