GHANA COLLEGE

Departments: Laboratory technology and public health

Introduction To Microbiology and Parasitology
Duration 2 Hours
Maximum marks 90
Instructions: Answer all questions, read questions carefully before answering, each question has four
options, out of which ONLY one is correct, select the correct one by entering it on the answer sheet
provided

1. Is chain forming, &round shaped C.Neisseria

bacteria. D. Salmonella
A. Pneumococcus 8. Antibiotic penicillin was introduced by:
B. Streptococcus A. Pasteur
C. Staphylococcus B. Fleming
D. Diplococcus C. Jenner
2. Which of the following is obligate anaerobe? D. Lister
A. Clostridium 9. Prokaryotic cell lacks:
B. Bacillus A. DNA
C. Staphylococcus B. Ribosomes
D. Klebsiella C. Mitochondria
3. Fungi that possess a capsule is: D. Plasma membrane
A. Candida
B. Aspergillus 10.All structures are external to cell wall
C. Cryptococcus EXCEPT:
D. Mucor A. Glycocalyx
4. Viruses do not contain: B. Flagella
A. DNA C. Pilli
B. RNA D. Spores
C. Enzyme 11.All of the following bacteria are members of
D. Cell wall family Enterobacteriaceae EXCEPT:
5. Viruses are: A. E. coli

A. Obligate intracellular parasites B. Shigella

B. Have their own metabolism C. Staphylococcus

C. Divide by binary fission D. Salmonella

D. Have both DNA and RNA 12.Mesophilic bacteria CANNOT grow at

6. Which one is an essential part of life cycle of temperature:
Toxoplasma gondii is spread by: A. 16oC
o
A. Dog B. 25 C
o
B. Cat C. 35 C
o
C. Human D. 38 C

D. Sheep 13.Facultative anaerobic bacteria grow in

7. Gram negative cocci are: presence of:
A. Staphylococcus A. Only O2

B. Streptococcus B. O2 and also CO2

 C. Only CO2 C. Bacillus
D. N2 D. Diplococcus

14. Protein particles which can cause disease 22. Temperatureofautoclaveat15psiwillbe .

o
are: A. 110 C
o
A. Virions B. 115 C
o
B. Nucleoida C. 121 C
o
C. Bacteriophages D. 125 C

D. Prions 23. Can cause food INTOXICATION?

15. Bacteria are more sensitive to A. Staphylococcus aureus

antibiotics at which phase of growth B. Streptococcus pyogenes

curve? C. E. coli

A. Decline phase D. Salmonella

B. Lag phase 24. Reproduction in bacteria occurs by:

C. Log phase A. Budding
D. Stationary phase B. Bursting
16. Syphilis is a bacterial STD, caused by . C. Binary Fission

A. Trichomonas vaginalis D. Fragmentation

B. Treponema pallidum 25. Bacteria eating viruses are known as:

C. Leptospira A. Phagocytes
D. Neisseria gonorrhoea B. Viricides

17. Which of the following has C. Prophages

optimum growth temperature D. Bacteriophages

greater than 45oC? 26. The Kingdom of recyclers is known as

A. Mesophilic
.
A. Algae
B. Psychrophiles
B. Bacteria
C. Psychrophilic
C. Fungi
D. Thermophiles
D. Embryophyte
18. Helicobacter pylori possess that
helps to neutralize stomach acid (HCl). 27. Timetakenforabacteriumtomultiplefrom1t
A. Coagulase
o2?
A. Incubation time
B. Urease
B. Growth rate
C. Hyaluronidase
C. Gene ration time
D. Catalase
D. Both “B” and “C”
19. Mycotoxins are produced by:
A. Bacteria
28. First phase of a bacterial growth curve is
A. Log phase
B. Viruses
B. Lag phase
C. Fungi
C. γ-phase
D. Protozoa
D. Exponential
20. Is the killing of all microorganisms both
in vegetative and sporing states. 29. Bacterial cells multiply rapidly during:
A. Disinfection A. Lag phase

B. Pasteurization B. Log phase

C. Sterilization C. Death phase

D. Antisepsis D. Stationary phase

21. Is Gram positive spherical &chain 30. Most human pathogenic bacteria are:
forming. A. Psychrophiles

A. Streptococcus B. Psychrotrophes

B. Staphylococcus C. Thermophiles
 D. Mesophiles C. Candida albicans
31. Malaria is caused by pathogen known as D. Penicillium
. 40. Compound microscope was discovered by
A. Plasmodium A. Antony von
B. Paramecium
B. Pasteur
C. Pseudomonas
C. Johnsen & Hans
D. Pasteurella
D. None of these
32. Viruses are:
A. Living 41. Father of Medical Microbiology is
B. Non-Living A. Pasteur
C. Only living inside cells B. Jenner
D. Capsular
C. Koch
D. A. L. Hock
33. Bacterium is type of organism.
A. Prokaryotic
42. According to Pasteur statements
which one of the following is true
B. Eukaryotic
C. Acellular
A. Living organisms discriminate between
D. May be prokaryotic or eukaryotic
stereoisomers
34. Tuberculosis is spread by: B. Fermentation is a aerobic process
A. Contaminated fomites C. Living organisms doesn’t discriminate
B. Food between stereoisomers
C. Respiratory droplets D. Both a and b
D. Vectors 43. Who demonstrated that open
35. Clostridium difficile is associated with: tubes of broth remained free of bacteria
when air was free of dust.
A. Myonecrosis
B. Food poisoning
A. Spallanzani
C. Antibiotic-induced colitis B. John Tyndall
D. Gas gangrene C. Francisco Redi
36. Gram positive bacteria are characterized D. Pasteur
by all of the following EXCEPT:
44. The principal light- trapping pigment
A. Thicker
molecule in plants, Algae, and cyanobacteria
B. Absent lipids is
C. Absent Teichoic acid
A. Chlorophyll
D. Absence of aromatic amino acids
B. Chlorophyll
37. For virus, choose the
WRONGSTATEMENT: C. Porphyrin
A. The viral envelope contains lipoprotein D. Rhodopsin
B. Viruses can replicate in non-living media 45. Tuberculosis is a
C. Viruses need live cells to grow
A. Water borne disease
D. Viruses have no ribosome
B. Air borne disease
38. After Gram’s staining, Gram positive
C. Food borne disease
bacteria are:
D. Arthropod borne disease
A. Green
B. Yellow 46. The main feature of prokaryotic organism
is
C. Red
D. Blue/violet A. Absence of locomotion
39. Example of yeast is B. Absence of nuclear envelope
A. Mucor C. Absence of nuclear material
B. Rhizopus D. Absence of protein synthesis
47. Mesosomas are also known as B. Morphology
A. Mitochondria C. Glucose fermentation Vs Respiration
B. Endoplasmic reticulum D. All of the above
C. Plasmids 55. Staining material of gram-
D. Chondroids p o s i t i v e bacterium is
48. Antiseptic methods were first introduced A. Fast green
by
B. Haematoxylin
A. Lord Lister
C. Crystal violet
B. Iwanowski

C. Beijernick
D. Safranin
D. Edward Jenner 56. Virion means
49. The capacity of a given strain of A. Infectious virus particles
microbial species to produce disease is B. Non-infectious particles
known as C. Incomplete particles
A. Pathogen D. Defective virus particles
B. Virulence 57. A facultative anaerobic is
C. Infection A. Only grow anaerobically
D. None of these B. Only grow in the presence of O2
50. The inventor of Microscope is C. Ordinarily an anaerobe but can grow
A. Galileo with O2
B. Antony von D. Ordinarily an aerobe but can grow in
absence of O2
C. Pasteur
58. The percentage of O 2 required
D. Koch by moderate anaerobe is
51. Neisseria gonorrhoeae was first A. 0%
described by
B. < 0.5%
A. Neisser in 1879
C. 2 – 8%
B. Pasteur in 1878
D. 5 – 10%
C. Robert Koch
59. The differences between Gram
D. None of these positive and Gram-negative bacteria is
52. B. anthracis was isolated by shown to reside in the
A. Cell wall
A. Louis Pasteur
B. Robert Koch B. Nucleus

C. Antony von Leeuwen hook C. Cell membrane

D. None of these D. Mesosomes

53. The bacterial cells are at their 60. Teichoic acids and Teichuronic acids
metabolic peak during are found in
A. Lag phase A. Gram positive bacteria
B. Log B. Gram negative bacteria
C. Fungi
C. Stationary
D. None of these
D. Decline
61. Lipid contents are more in
54. How would you distinguish
pseudomonas species from E-coli? A. Gram negative bacteria
B. Gram positive bacteria
A. Gram staining
C. Same in both
D. None of these 69. Symbionts are
62. Cell-wall is A. Bacteria in symbiotic association
A. Thick in Gram positive than Gram B. The group of fungi in symbiotic
negative association
B. Thick in Gram negative than Gram C. The groups participating in symbiotic
positive association
C. Equal in both D. All of these
D. In Gram negative cell-wall is absent 70. Alkaliphiles grow at pH value
63. Gram negative bacteria appear as between
A. 1 to 6
A. Pink
B. 6 to 9
B. Violet
C. 1 to 11
C. both a & b
D. 7 to 12
D. None of these
71. The micro-organisms grow at
64. The molds obtained nutrition from high salinity are
dead and decaying matter which are called
A. Osmophiles
A. Saprophytes
B. Halophiles
B. Parasites
C. Both a and b
C. Commensals
D. None of these
D. None of these
72. Bacteria which need oxygen for
65. The inorganic forms of nitrogen, growth are called
which are accepted by bacteria are
A. Thermophilic bacteria
A. Nitrates
B. Microaerophilic bacteria
B. Nitrites
C. Facultative anaerobic bacteria
C. Ammonium salts D. Mycobacteria
D. All of these 73. If the source of energy for bacteria is
66. The smallest virus is from chemical compounds they are said to
be
A. Parvo virus
A. Phototrophs
B. Rhabdoid virus
B. Autotrophs
C. Pox virus C. Chemotrophs
D. Adeno virus D. Chemolithotroph
67. Nitrite is converted into nitrate by 74. The reproduction rate is equal to
the bacteria death rate in which stage
A. Nitrosomonas A. Decline phase
B. Neutrocytes B. Stationary phase
C. Nitrobacter C. Lag phase
D. Azotobacters D. Log phase
68. The group of bacteria which depends 75. Optimum growth temperature is
on organic sources in nature for their energy greater that 45oC is
requirements. They are said to be
A. Mesophiles
A. Chemotrophs
B. Thermophiles
B. Phototrophs
C. Psychrophiles
C. Heterotrophs
D. None of these
D. Organotrophs
 76. The organisms which can grow B. Spallanzani
both in presence and absence of oxygen
C. Schwann
A. Aerobes
B. Anaerobes D. John Snow
C. Facultative anaerobes 83. Which microorganism causes gonorrhoea?
D. Strict aerobes
A. Salmonella typhi
77. The organisms which can grow
best in the presence of a low B. Corynebacterium diphtheriae
concentration of oxygen
C. Neisseria gonorrhoea
A. Aerophilic
D. Rickettsia
B. Microaerophilic
C. Aerobic 84. Which scientist proposed the Three-Domain
System based on 16S rRNA sequencing?
D. Anaerobic
78. Who discovered that cowpox virus could A. Carl Linnaeus
protect against smallpox?
A. Robert Koch B. Louis Pasteur

B. Edward Jenner C. Carl Woese

C. Howard Ricketts D. Antonie van Leeuwenhoek
D. Dimitri Ivanovsky
79. What does "ubiquitous microbes" mean? 85. Which of the following is NOT a
A. Microbes are found only in specific characteristic of Archaea?
environments.
B. Microbes are found everywhere on Earth A. Lack peptidoglycan in cell walls
and in/on the body.
B. Possess unique membrane lipids (ether-linked)
C. Microbes are limited to aquatic
environments only. C. Often found in extreme environments
D. None of the above
D. Contain peptidoglycan in their cell walls
80. Which scientist demonstrated that cholera
spreads through contaminated water? 86. Which group of fungi produces sexual
A. John Hunter spores in sac-like structures called asci?

B. John Snow A. Basidiomycota

C. Robert Koch B. Ascomycota

D. Louis Pasteur C. Zygomycota
81. What is the germ theory of disease? D. Chytridiomycota
A Diseases are caused by non-living particles. 87. Based on the Baltimore Classification
B Contagious diseases are transmitted by System, which group includes viruses like
microbes. HIV?
C Diseases arise spontaneously in organic A. Group I (Double-stranded DNA viruses)
matter.
B. Group IV (Positive-sense single-stranded RNA
D All diseases are genetic in origin.
viruses)
82. Who introduced the concept of sterile
culture media? C. Group VI (RNA reverse transcribing viruses)

A. Pasteur D. Group II (Single-stranded DNA viruses)

88. Which of the following criteria is used for
Gram staining classification of bacteria?
A. Oxygen requirements
B. Temperature preferences
C. Cell wall composition
D. Nutritional requirements
89.Which of the following is the smallest known
infectious agent?
A. Viruses
B. Viroids
C. Prions
D. Bacteria
90. Which molecular method is considered the
gold standard for bacterial and archaeal
identification?
A. Whole Genome Sequencing
B. 16S rRNA Gene Sequencing
C. Multilogues Sequence Typing
D. Proteomics