The Chemistry and Biochemistry of
Brewing Beer
Glucose
H2 Glycolysis
NAD+
C 2 ATP
NADH NADH NAD+
H3C OH Pyruvate
PDC
Acetaldehyde
ADH
Ethanol
Respiration Alcoholic Fermentation
Beer Composition
Water 92%
Ethyl alcohol 4.4%
Carbohydrates 3.0%
Carbon dioxide 0.5%
Protein 0.3%
plus hundreds of others
Required Ingredients of Beer
• Water • Malt Barley
• Hops • Yeast
The Brewing Process
Milling
Mashing
Lautering
Boiling
Fermenting
Conditioning
The Brewing Process
Milling – Choice of Malt
Milling – SRM Color
Milling – SRM Color
Milling
• The dried malt is milled to crack open the grains, exposing starches for sugar
conversion during mashing.
Malt Grist
The Brewing Process
Mashing – Mash Tun
Mashing - Gelatinization of Starch
Mashing - Gelatinization of Starch
Mashing - Gelatinization of Starch
Mashing - Saccharification
• The α- and β-amylases convert the starch molecules
of the malt into sugars used in fermentation.
gelatinization liquefaction saccharification
A close-up view of grains
steeping in warm water during Optimal rest temperatures for major mashing enzymes
the mashing stage of brewing
Mashing – Protein Denaturation and Cleavage
• The proteases and heat convert the protein molecules of the malt into
amino acids used in fermentation.
Heat
Heat
Mashing – Protein Denaturation and Cleavage
How is Beer Made? - Easy Guide to Mashing KPU Brewing School
https://youtu.be/6xHYNak64bY
The Brewing Process
Lautering – Vorlaufing
Lautering – Vorlaufing and Sparging
How is Beer Made? - Lautering (Wort Separation) Process - KPU Brewing School
https://youtu.be/UOcOiBe6pa8?si=jvyYxh_aFCQXkXEo
Lautering – Spent Mash
The Brewing Process
Wort Boiling Objectives
• Wort Sterilization
• Enzyme Deactivation
• Protein Coagulation
• Color and Flavor Development
• Wort Concentration by Evaporation
• Hop extraction and Isomerization
• Evaporation of Volatiles
• Ph Reduction
Wort Boiling – Hops Addition
Boiling – Isomerization of alpha Acids
Boiling – Isomerization of alpha Acids
Boiling – Isomerization of alpha Acids
Boiling
How is Beer Made? - Wort Boiling Process (Brew Kettle & Whirlpool) – KPU Brewing School
https://youtu.be/D164GVcN4Bo
Specific Gravity
Specific Gravity - Hydrometer
How to Use a Hydrometer for Homebrewing - NorthernBrewerTV
https://youtu.be/jNVePBvI2ps
The Brewing Process
Chilling
How is Beer Made? - Wort Cooling & Transfer to FV = KPU Brewing School
https://youtu.be/d07IfHO5k20