Company Information

© Copyright 2021. Epredia. All rights reserved. These instruments conform to the essential
requirements of:
Epredia makes every attempt to ensure that the information
contained in this supporting document is correct and clearly • In Vitro Diagnostic Directive 98/79/EC
stated, but does not accept responsibility for any errors or • Machinery Directive 2006/42/EC
omissions. The development of Epredia products and services is
an ongoing process. Please ensure that any published Symbols
information you use as a reference is up to date and relates to
the condition of the product. If necessary, check with your local The following symbols and conventions may be
Epredia representative. used throughout this document and on the
instrument:
This document may not, in whole or in part, be copied,
photocopied, reproduced, translated, or converted to any This symbol is used on the equipment, or
electronic or other form without prior written consent of Epredia. in a document, to indicate that instructions
All information contained in this manual is proprietary and must be followed for safe and correct
confidential, and the exclusive property of Epredia and is operation.
protected by copyright. This symbol is also used on the instrument,
or in a document, to indicate that irritants
or potentially harmful chemicals are
present. Refer to the Material Safety Data
Sheets for the products, and always use
Good Laboratory Practice.

If this symbol appears on the instrument

always refer to the operator guide.

This symbol is utilised on the instrument, or

Contact address in a document, to indicate that there are
potential biological risks associated with
Shandon Diagnostics Limited, a subsidiary of Epredia
the instrument and / or with instrument
Tudor Road, Manor Park, Runcorn
use. Always use Good Laboratory
Cheshire, WA7 1TA, UK Practice.
Tel: +44 (0) 1928 534000
Fax: +44 (0) 1928 534001 Single Use.
Web: www. epredia.com
This symbol is used on the instrument, or in
the document, to indicate that instructions
for use must be consulted

Manufacturer

This symbol indicates the product is an In

Vitro Diagnostic Medical device according
USA Distributor to European Directive 98/79/EC.

Richard-Allan ScientificLLC, a subsidiary of Epredia A warning is given in the documentation if there is a

4481 Campus Drive potential risk of injury, equipment failure or poor
Kalamazoo, MI 49008, USA tissue sample processing outcome.
Tel: 1-800-522-7270
Note
Fax: +1 269-372-2674
Web: www. epredia.com Notes give additional information about a job or
instruction, but do not form part of the instruction.
Contents
Company Information 3 Out-Of-Balance Tests ................................19

Symbols ...................................................3 Suspension System Test ............................19

Contact address.........................................3 Lid Lock Test ...........................................19

USA Distributor ..........................................3 Processor And Interface PCB Test ...............19

Display PCB And Keyboard Test..................20
Contents 4
EMC Statement..........................................6
Chapter 5 - Troubleshooting 22
General ...................................................22
Safety Information 7 Flowchart 1..............................................29
General Safety ...........................................7 Flowchart 2..............................................30
Chemical Safety .........................................8 Flowchart 3..............................................31
Environment ..............................................8 Flowchart 4..............................................32
Warranty Statement ....................................8 Flowchart 5..............................................33

Chapter 1 – Introduction to Flowchart 6..............................................34

Cytospin 4 9 Flowchart 7..............................................35

IVD Intended Use........................................9 Chapter 6 – Parts Replacement 36

Introduction to the Cytospin 4 .......................9
Tool/Material Requirements ........................36
Safety.......................................................9
Torque settings ........................................36
Service .....................................................9
Plinth ......................................................37
Operation................................................ 10
To separate and re-join the case and plinth (1)38
Chapter 2 – Technical Description11 To separate and re-join the case and plinth (2)39

General................................................... 11 To replace the moulded plinth .....................40

Basic Structure ........................................ 11 To replace the Out-of-Balance microswitches 41

Electronics Assembly................................. 12 To replace the motor controller....................42

To replace the motor .................................43
Chapter 3 – Production Services15 To replace the suspension frame .................44
Introduction ............................................. 15 To replace the wire rope mounts..................45
Production Services .................................. 15 To replace the centralising springs ...............46
Mode 1 - Out-of-Balance checks................. 15 To replace the Processor and Interface PCB ..47
Mode 2 - Speed Control and Acceleration..... 17 To replace the mains inlet assembly .............48
Software Updates..................................... 17 To replace the Power Supply Unit ................49
Case ......................................................50
Chapter 4 - Functional Tests 18
To replace the Display PCB ........................51
Mains Input and PSU Test.......................... 18
To replace the keyboard.............................52
Motor Test .............................................. 18
To remove the splash guard........................53

Cytospin 4 Service Manual Issue 5 4

 To replace the lid lock assembly .................. 54
To change the lid lock assembly components 55
To replace the bump stops......................... 56
Lid ......................................................... 57
To remove and replace the lid ..................... 58
To replace the inner liner and seal assembly .. 59
To replace the inner liner seal ...................... 60
Sealed Head............................................ 61
To replace the Sealed Head Hub ................. 61
To replace the Sealed Head lock assembly.... 62

Chapter 7 – Specification,
Accessories and Spares 63
Specification............................................ 63
Accessories............................................. 65
Spares List .............................................. 67

Chapter 8 – Drawings 72
Electrical Drawings.................................... 72

Chapter 9 - Routine Service

Instructions 77
Annual Service ......................................... 77

Appendices 82
Appendix A Transportation Instructions......... 82
Appendix B Approved Reagent List.............. 83
Appendix C Cleaning and Maintenance......... 83
Appendix D Technical Tips and Service Bulletins89

Index 90

Cytospin 4 Service Manual Issue 5 5

EMC Statement
This IVD equipment complies with the emissions
and immunity requirements of IEC 61326-2-
6:2006.
This equipment has been designed and tested
to CISPR 11 Class A.
It is intended for use in a laboratory environment
by qualified and trained laboratory personnel
only. In a domestic environment it may cause
radio interference, in which case it may be
necessary to take measures to mitigate the
interference.

Cytospin 4 Service Manual Issue 5 6

Safety Information
Epredia instruments are designed for convenient General Safety
and reliable service; however, improper use or
handling by a user may damage the instrument This instrument, as supplied,
or cause a hazard to health. The instrument conforms to IEC61010-1 and
must not be used in a manner not specified by IEC61010-2-101; however, the
Epredia Correct maintenance procedures are addition of chemicals introduces
essential for consistent performance. It is potential hazards. Good Laboratory
recommended that users secure a maintenance Practice must be employed and
contract with our service department. consideration must be given to the
To remain compliant with regulatory potential for hazard when dealing
requirements, and to ensure that mandatory with these chemicals.
safety upgrades are performed at the earliest
opportunity, it is strongly recommended that all Do not use this instrument in close
service activities are performed by Epredia- proximity to strong electromagnetic
factory trained Engineers. Warranty may be radiation as this may interfere with
voided if service is performed by non-factory the proper operation. The
trained Engineers. electromagnetic environment should
be evaluated prior to operation of the
Maintenance or repairs that are not performed
device.
by Epredia trained Engineers with proven
training may affect the safety, performance and Good Laboratory Practice must be
compliance of the equipment. used when handling tissue samples
Please consult your local sales or support teams to prevent cross contamination and
for more information about service contracts. infection. The user should complete
a risk assessment to determine any
potential hazards related to tissue
The following sections contain
handling.
important information for the safe
setup and use of the instrument, The Cytospin bioseals and related
and should be read and understood components are intended to be part
by the user before using the of the bio-containment systems such
instrument. as are specified in International and
National bio-safety guidelines, and
cannot be relied upon as the only
means of safeguarding workers and
the environment when handling
pathogenic microorganisms.

Cytospin 4 Service Manual Issue 5 7

Chemical Safety Environment
The introduction of chemicals creates potential This instrument is required to comply with the
hazards. Epredia has adopted the following European Union's Waste Electrical and
position with regard to the subject of volatile Electronic Instrument (WEEE) Directive
chemicals used in laboratories: 2012/19/EU. It is marked with the following
symbol:
• Customers using non-specified
chemicals in the instrument do so
at their own risk.
• All chemicals recommended by
Epredia have auto-ignition
temperatures considerably above
Epredia has contracts with one or more
any surface temperature that can
be reached during a single fault recycling / disposal companies in each EU
failure on the instrument. Member State, and this product should be
disposed of or recycled through them. For
• The instrument contains no
further information contact your Epredia service
source of ignition in any areas of representative
the instrument where chemicals
are stored, or are likely to leak
into in a single fault condition.
Warranty Statement
• The operator is fully aware of the Epredia is proud of their quality, reliability and
contents of the specification after-sales services. We continuously strive to
documents detailing the improve our service to our customers.
properties of the chemicals they Please ask your distributor or Epredia
are using. representative about service contracts which
• The operator has carried out any can help maintain your instrument in an optimal
legally required assessment of operating condition.
chemicals used and is using good
Warranty provisions necessarily vary to comply
laboratory practice.
with differences in national and regional
legislation. Specific details can be found in the
delivery documentation or from your dealer or
representative.
Please note that your warranty may be
invalidated if:
• This instrument is modified in any way, or not
used as intended by Epredia
• Accessories and reagents which have not
been approved by Epredia are used.
• The instrument is not operated or maintained
in accordance with instructions.
• The installation of the instrument was not
conducted by a certified Epredia
representative.

Cytospin 4 Service Manual Issue 5 8

Chapter 1 – Introduction to
Cytospin 4
IVD Intended Use health. It is important for you to obey the
following safety precautions:

This paragraph details important

safety information. please read this
section carefully

The Cytospin 4, as supplied, conforms with

The Cytospin 4 is an in vitro diagnostic device. IEC1010-2-020.
The Cytospin 4 is a self-contained cyto- Anybody who uses or services the instrument
centrifuge whose primary function is to produce must read and understand the Operator Guide
a monolayer of cells onto a glass slide from any (A78310250) that is supplied with the
fluid suspension. equipment. The instrument must be operated in
The Cytospin 4 is designed for use in Pathology accordance with the instructions given in the
Operator Guide.
Laboratories and only trained and qualified
laboratory personnel may operate the The instrument must be serviced by a Epredia
Cytospin 4. trained engineer in accordance with the
instructions contained in this Service manual
Introduction to the Cytospin 4 (A78310251).

The purpose of the Service Manual is to enable Understand the functions and construction of
a qualified Epredia trained engineer to maintain the Cytospin 4 before you carry out any work on
the Cytospin® 4 cytocentrifuge in good the instrument.
condition. If the instrument has been used with materials
The features, components and modules of the that are toxic or contaminated with pathogenic
Cytospin® 4 cytocentrifuge are detailed in micro-organisms, follow the cleaning
Section 2. instructions given in Appendix C. The Product
Return Certificate (found in Chapter 9 – Routine
Diagrams and illustrations are used in this
Service Instructions) must be completed and
manual to help you locate components and
signed by the user before the instrument is
instructions are given about how to replace
serviced or returned to Epredia.
parts. Use the Fault Finding Charts to help you
to locate problems and repair a fault.
Service
Safety • Hazardous and potentially lethal voltages
above 110V a.c. or 50V d.c. are present
Epredia products are designed for convenient
inside the instrument. Do not undo or
and reliable operation and to accepted
remove any access covers unless you are
standards of safety. The use of the Cytospin 4
trained to do so. Before you do any work
does not entail any hazard if operated in
inside an area enclosed by an access panel,
accordance with the instructions.
make sure that the mains power is switched
However, incorrect actions by a user may off at the supply and at the instrument and
damage the equipment, or cause a hazard to the mains lead is disconnected. Always
follow the safety instructions.
• Wear earthing (grounding) wrist straps and • Lifting Positions
earth (ground) yourself before you handle Cytospin 4 weighs approximately 12
connectors or electronic components. Some kilograms (26.5 lbs.). If necessary, get help
electronic components will fail if damaged by to safely move or lift the instrument without
static electricity from your body. Damage risk of injury.
from static electricity may take weeks or
months to develop. To lift or move Cytospin 4, hold the
instrument securely under both sides.
• Make sure that the instrument is properly
connected to a good earth (ground) contact.
All earth (ground) straps and connections
MUST be replaced after service and before
the instrument is powered up.
• Make sure that no fingers or articles of
clothing are allowed within the area defined
by the plinth if the Cytospin 4 has to be
operated while the cover is removed.
Components may suddenly move under
computer control and cause injury.
• Use only factory approved accessories or
replacement parts with the Cytospin 4.

Operation
• It is very important that you use normal
standards of safety and good laboratory
practices at all times. Think about your
actions: use common sense and the best
known practice when operating the
instrument.
• Cytospin 4 is not intended for use with
flammable materials or solvents, nor with
materials which could be explosive or
chemically reactive.
• Always load and unload the Sealed Head in
a biologically safe fume cupboard. Assume
that the samples are biologically hazardous.
• Bioseals and other biosafety components
cannot be relied upon as a sole safeguard
against contamination by pathogenic
microorganisms.
• Refer to laboratory procedures and
manufacturer’s data sheets when using
reagents. Only use reagents recommended
in Appendix D of the Operator Guide and
Appendix B of this Service Manual.

Cytospin 4 Service Manual Issue 5 10

Chapter 2 – Technical Description
General Basic Structure
The Cytospin 4 cytocentrifuge is a precision The basic structure consists of a motor
engineered, self-contained cytocentrifuge mounted on a suspension frame within a plastic
whose primary function is to produce a moulded enclosure. The motor provides direct
monolayer of cells onto a glass slide from any drive to the Sealed Head which contains the
fluid suspension under the safest conditions specimens to be processed.
possible The different components of the
Cytospin 4 centrifuge are shown in the diagram Plinth Assembly
below..
The plinth assembly consists of a moulded
plinth onto which are mounted the Power
Supply, Processor and Interface PCB, Motor
Controller, Out-of-Balance sensors, and the
Suspension Frame and Motor.

Suspension Frame and Motor

The motor is directly mounted on the
suspension frame and provides direct drive to
the Sealed Head through a tapered drive boss.

The suspension frame is mounted on 4 wire

rope anti-vibration mounts - one at each corner.
There are also 4 tension springs running
between the suspension frame and plinth. The
wire rope anti-vibration mounts provide the
Special design features of the Cytospin 4 damping between the Sealed Head /
include: suspension frame and the plinth. The tension
springs make sure the suspension frame sits in
• Lid release mechanism that allows for easy
a central position.
one-handed opening of the Cytospin 4 to
load and unload the sealed head. Case and Lid Assembly
• New control panel for easy user interface.
The case and lid are both plastic Mouldings
• An intuitive way to set programs and interact which are easily removed from the Plinth
with all Cytospin 4 applications. assembly to allow easy access for servicing.
• Additional visual and audible indicators that
The case houses the Lid Lock assembly and
help to trouble-shoot the instrument.
Keyboard / Display unit.
• Fully programmable memory - up to 23
programs can be easily stored and recalled. The lid has an inner liner that provides an easy
clean surface as well as housing the lid seal.
• A Pull-out Program Card allows you to easily
The lid latch is also mounted in the lid. 2 large
reference each program.
• Power Save feature. torsion springs provide the damping for the lid.
Lid Lock Assembly crystal. Integrated memory provides program
storage for up to 23 user programs. Programs
The Lid Lock assembly consists of 2 sensors
can be loaded, modified and saved using the
and a solenoid mounted on a bracket. The
keyboard / display. The software program is
position of the bracket within the case can be
stored in flash memory and can be updated by
adjusted to ensure correct alignment with the
downloading from a PC via a serial link, refer to
latch.
Production Service section.
A small switch is located under the locking pin,
The microcontroller has RAM available for
which is actuated by the lid latch when the lid is
temporary memory storage, watchdog
closed, and provides the feedback to the
monitoring and Power On Reset.
software that the lid is closed.
The +24V d.c. is fused (T3.15A) on the PCB and
The solenoid is actuated whenever the motor is
is supplied to the on-board regulators, and via a
started and locks the lid latch under the locking
transistor to the motor controller. The transistor
pin. The optical sensor at the rear of the
enables the microcontroller to control the power
solenoid detects the position of the solenoid
to the motor. The power to the motor is cut if
plunger and provides feedback that the lid is
out-of-balance, overspeed or lid open is
actually locked. The solenoid automatically
detected whilst the motor is running.
unlocks when the motor stops.
An on-board 1A switched regulator circuit is fed
Electronics Assembly from the +24V d.c. supply from the switched
mode power supply, and generates the +5V for
Overall Service Schematic A783 - 0105
all the logic circuitry within the instrument An
This assembly comprises the following elements on-board 100mA regulator is fed from the +24V
- Mains Input and Power Supply, Processor and d.c. supply from the switched mode power
Interface PCB, Display PCB, Keyboard, Motor supply and generates the +12V d.c. for the D/A
Controller and Motor, Lid Lock Assembly, O ut- converters for the motor speed control output,
of-Balance Sensors. and the frequency to voltage converter for the
overspeed circuit
The Mains Input and Power Supply, Processor
and Interface PCB, Motor Controller and Motor The speaker / alarm is fed directly from the
and Out-of-Balance Sensors are all located processor via a level shift circuit.
within the plinth (base) of the instrument.
The overspeed detection circuit uses a
The Display PCB, Keyboard and Lid Lock frequency to voltage converter circuit with a
assembly are located on the case assembly. precision capacitor for the ‘charge pump’ and
They are connected to the Processor and precision resistors for the load and comparator
Interface PCB via a 26 way ribbon cable. switch level. The frequency of switching is
controlled by these components, and provision
Processor and Interface PCB for a variable resistor has been made if the
Epredia part number A78310023 tolerance of these components does not give a
controlled switching range - overspeed should
Circuit Diagram A783-0107 Issue 2 be detected between 2300 and 2500 rpm. The
The Processor PCB contains the overspeed is latched to the processor and
microcontroller, voltage regulation, overspeed motor power control, and can only be cleared
detection, speaker / alarm and input / output by processor control.
control circuits to interface with the Motor The ‘actual’ speed signal is also read by the
Controller, Display PCB, Keyboard and Out-of- processor enabling it to control acceleration to
Balance sensors The microprocessor system is the set speed and maintain speed within ± 20
a 16 bit microcontroller driven by a 16 MHz

Cytospin 4 Service Manual Issue 5 12

rpm. Variances outside of this cause an error The speed and time displays are also used to
condition. show error codes. There are seven LEDs to
indicate selected acceleration rate, save
The motor controller requires two mode signals
program, lid locked and closed status and
to select disable, brake and drive direction. The
standby mode. The keyboard connections are
mode signals are gated with the lid lock sense
routed through the display PCB.
signal, so if for any reason the lid is unlocked,
the controller is forced to the brake. The Display PCB has interfaces to the lid lock
assembly consisting of:
The control voltage to the motor controller is
generated via two 8 bit DAC’s, the ‘hi’ byte DAC • Lid lock solenoid - the drive circuit takes the
generates an output voltage of 0 - 10 volts and +24V via a resettable fuse. The +24V is then
the ‘lo’ byte DAC generates a voltage of 0 - pulsed via a capacitor to the solenoid. The
2.56 volts to give fine adjustment. The two DAC solenoid is a ‘latching’ type, in that it remains
outputs are subtracted to give a control voltage in either position without power and requires
of 0 - 10 volts, the control range of the motor a +ve or -ve pulse to move from one position
controller. to the other.
The Processor and Interface PCB has interfaces • Lid lock sensor - this is an optical sensor to
to the following PCBs and assemblies: detect the position of the solenoid bolt
• Lid closed sensor - this is a microswitch
Motor Controller - 2 bit mode control output to
activated by the lid latch engaging if the
select disable, brake and drive direction; voltage
closed position
control output to set speed 0 – 10 volts; actual
speed input - 12 pulses per revolution; power to Keyboard
the motor controller is under the control of
processor, overspeed, out of balance and lid Epredia part number A78330038
closed sensors. The keyboard is a 3 row by 5 column matrix
Display PCB - 7 segment display and LED scanned by the microcontroller via I/O circuits
drivers; keyboard scanning interface 5 x 3; lid on the Processor and Interface PCB.
lock and closed, sense and control. The keyboard enables default and user
Out-of-Balance Sensors - dual circuits to programs to be selected, modified and saved,
process sensor states, two normally closed with scroll buttons to select program, speed
microswitches wired in series, so that if either and time. Separate buttons select the
one or both switches are activated, an open acceleration rate (low, medium or high). The
circuit condition is latched to the processor and [START] button activates the selected program
power control for the motor. which may be stopped with the [STOP] button.
The Standby button [ ] selects standby mode
Display PCB whereby all displays are turned off.
Epredia part number A78310022
Circuit Diagram A783-0106 Issue 1
General Circuit Overview:
The Display PCB contains the 7-segment
displays for program number (1), speed (4) and
time (2). The segment drive circuits for these are
on the Processor PCB; the display and LED
common anodes are driven on the Display PCB.

Cytospin 4 Service Manual Issue 5 13

 variation in specification. The ground terminal is
Motor Controller and Motor Assembly
connected to the metal casework of the PSU
• Epredia part numbers AP14339 (Motor and is designated as the ‘Protective Earth’ for
Controller) the unit.
• A78310014 (Motor Assembly)
The motor controller is a bought-in part
Autofixation
manufactured by Papst, designed to drive d. c. Original units had an Autofixation feature that
brushless motors. The controller requires a could be enabled from the key panel. For a
+24V supply and two mode inputs to select number of reasons, Epredia decided not to
disable, drive in anticlockwise or clockwise proceed with this feature (which also required
directions, and braking. special cytofunnels).
A voltage of 0 to +10V selects the required Note
speed of the motor. The controller generates That any replacement displays and keyboards
the required three phases to the motor and that are supplied will not have the Autofixation
power for the three hall effect sensors within the feature on them and the program to run this
motor. The hall effect devices sense the feature is disabled.
direction and rotation of the motor, and return
pulse streams relative to the speed of rotation.
The controller has an in built control loop w hich
senses the difference between requested and
actual speed and compensates the drive
phases accordingly to attain and control speed.
The three hall sensor signals are also combined
within the controller to give an ‘actual’ speed
signal out (12 pulses per revolution with the
frequency directly related to the speed of
rotation).
The brake mode selects an ‘active braking’
system which short circuits a current monitored
winding and is designed to reduce deceleration
times. The unit is fused and protected from
excessive currents and voltage surges during
braking. A power diode on the Processor PCB
protects the +24V supply.

Mains Input and Power Supply

The mains input is a standard IEC320 module
with a 10A filter, double fuse (2 x T5A) and
double pole switching.
The switched mains is fed directly to the switch
mode power supply, 24V, 3 amps with 6A
surge capability.
The power supply is capable of accepting 100-
240 volts nominal, 50/ 60Hz without any
Chapter 3 – Production Services
D Hardware
Introduction OOB Latch
Production Services is used to analyse and test
the Cytospin 4. E Lid Closed Switch

F Latched OOB1 Live OOB1

Production Services
G Mode 1 Mode 1
The following tests use the segments of the 7
segment display to show information. The
segments are annotated as shown below: Mode 1
The G segments on both digits light when the
instrument is in Mode 1 for the Out-of-B alance
checks.

Mode 1 - Out-of-Balance Lid Closed Switch

checks Segment E on the right hand digit lights when
Switch the instrument on and press [LOW] the software senses that the lid has been
Acceleration button at the same time. closed. The segment turns off when the
software senses that the lid is open.
The Program display will be blank, the Speed
display will show 0000 at start up and the Time
display will show 2 dashes in position G to
signify that the instrument in in Mode 1:

Each segment in the two Time Display digits

has a specific meaning detailed in the follow ing
table:

Segment Left Hand Digit Right Hand Digit

B Latched OOB2 Live OOB2

C Lid Locked Opto

Lid Locked Opto Hardware OOB Latch
Segment C on the right hand digit lights when Segment D of the left hand digit lights to show
the software senses that the lid has been that an Out-of-Balance has occurred. This
locked. The segment turns off when the signal is from the section of circuitry on the
software senses that the lid is unlocked. When Processor and Interface PCB that prevents the
this segment is lit, the Lid Closed Switch Motor from running when an Out-of-Balance
segment (right hand digit segment E) should occurs during normal operation, and shows that
also be lit. it is operating correctly.

Live OOB1 and OOB2

Segments B and F of the right hand digit act as
a live display showing the status of the Summary
horizontal or vertical Out-of-Balance switches. When the Out-of-Balance switches are first
When either switch is triggered, both segments triggered, four things happen:
will light. When the switches return to normal,
• The Live OOB1 and Live OOB2 segments
both segments will turn off again.
(right hand digit segments B and F) will light
momentarily until the switches are returned
to normal.

Latched OOB1 and OOB2

Segments B and F of the left hand digit give a
• The Latched OOB1 and OOB2 segments
permanent display to show that the Out-of-
(left hand digit segments B and F) light.
Balance switches have been triggered. The
speed display will also show the speed at which
the Out-of- Balance occurred.
These will only be cleared when the instrument
is turned off and on again.

• The speed display shows the speed at which

the Out-of-Balance occurred

• Left hand digit segment D lights.

Cytospin 4 Service Manual Issue 5 16

 Note
The actual speed is displayed during steps 2 to
5.

Software Updates
To update the software on the Cytospin 4, the
Mode 2 - Speed Control and following are required:
Acceleration PC with a working serial port (COM 1)
Switch the instrument on and press [MED] Software update disc (Epredia part number
Acceleration button at the same time. A78310260)
Segment G of the Program display will be lit, the Serial connection lead (Epredia part number
Speed display will show 0000 at start up and 75220418)
the Time display will show the time taken to
Disconnect the Cytospin from the power.
accelerate up to the programmed speed (in
Remove the power cord from the instrument.
numbers of ½ seconds).
Separate the case and plinth as described in
For the automatic program, the instrument w ill
Chapter 6, To separate and re-join the case and
use the speed, acceleration and time of the last
plinth section to access the Processor and
program run.
Interface PCB.

Use the serial lead (75220418) to connect the

COM1 serial port of your PC to the serial port of
the Processor and Interface PCB (PL4).
Re-connect the instrument to the mains pow er
supply but do not switch on the instrument.
The software update program can only be run
from the MS-DOS prompt on your PC, so either
boot the computer into MS-DOS mode, or
access the command prompt window from
within Windows.
Change the directory to access the floppy disc
and run the program HC12RCU.EXE. The
program will prompt you to switch on the
The automatic program will do the following: Cytospin and will display the status as the
• Start software update takes place.
• Accelerate up to the set Speed (note that the Once the update is complete, switch off the
Time display shows the time taken to reach instrument and disconnect the power cord from
the set speed in numbers of ½ seconds) the supply and from the instrument. Remove the
• Hold for the set Time serial lead and reassemble the instrument (see
• Decelerate Chapter 6 To separate and re-join the case and
plinth section).
• Stop
• Increment the set Speed by 100 rpm (up to Carry out the Processor and Interface PCB test
a maximum of 2000 rpm) as detailed in Chapter 4 - Functional Tests.

Cytospin 4 Service Manual Issue 5 17

Chapter 4 - Functional Tests
Mains Input and PSU Test Motor Test
If the Mains Input socket assembly and/or the If the Motor and/or Motor Controller has been
PSU have been replaced, carry out the following serviced, carry out the following tests:
test:
Test 1
Check that the correct fuses (2 x T5A) are fitted
Load an empty Sealed Head into the Cytospin.
to the mains socket.
Program the Cytospin 4 as follows:
Check the polarity of the connections to the
PSU and between the PSU and Processor and • Acceleration High
Interface PCB - • Speed 2000 rpm
• PL3 / 1 +V • Time 2 minutes
• PL3 / 2 -V Press [START] and check that the speed
Carry out the Earth Continuity and Insulation reaches 2000 rpm within 25 seconds ± 5
Resistance Electrical Safety Tests. Use the PSU seconds.
frame or the fixing screw underneath the Monitor the speed with a Tachometer and make
instrument for the earth connection. Earth sure that the speed is maintained at 2000 rpm ±
Continuity should measure less than 0.1Ω; 20 rpm for 1 minute.
Insulation Resistance should measure greater
Press [STOP] and check the instrument
than 2MΩ.
decelerates to 0 rpm within 32 seconds ± 5
Switch the instrument on. Check that there is seconds.
+24V ± 200mV between TP3 (+24V) and TP2
Test 2
(0V).
Load an empty Sealed Head into the Cytospin.
If necessary, adjust the potentiometer on the
PSU to give the correct voltage. However, note Program the Cytospin 4 as follows:
that the PSU is calibrated when supplied and • Acceleration Low
the potentiometer is very sensitive.
• Speed 200 rpm
Load an empty Sealed Head into the Cytospin. • Time 1 minute
Program the Cytospin 4 as follows: Switch the instrument off.
• Acceleration High Select Production Services Test Mode 2 -
• Speed 2000 rpm switch the instrument on again while pressing
• Time 2 minutes [MED.] at the same time.

Press [START] and check that the speed Press [START]. The instrument will run a cycle
reaches 2000 rpm within 25 seconds ± 5 using speeds from 200 to 2000 rpm in steps of
seconds. 100 rpm, stopping and restarting at each speed
change. This will take approximately 15 minutes
Monitor the speed with a Tachometer and make
to reach 1000 rpm.
sure that the speed is maintained at 2000 rpm ±
20 rpm for 1 minute. Check that there are no errors and that the
instrument does not stop.
Press [STOP] and check the instrument
decelerates to 0 rpm within 32 seconds ± 5 Press [STOP]. Switch the instrument off when
seconds. the Sealed Head stops spinning.
Note
Suspension System Test
The test can be stopped once the speed has
If the suspension system has been serviced,
reached 1000 rpm.
carry out the following test:
Out-Of-Balance Tests Fit 1 Cytoclip with a glass slide and funnel and
If the Out-of-Balance sensors have been another with only a funnel (no slide). Load these
serviced, carry out the following tests: Cytoclips into a Sealed Head in opposite
positions (for example positions 1 and 7). The
Out-of-Balance Switches difference between the two Cytoclips will give
approximately 5g Out-of-Balance.
Enter Production Services Test Mode 1.
Check that the instrument operates smoothly up
Move the suspension frame by hand to operate to 2000 rpm on low acceleration.
each Out-of-Balance sensor in turn. Make sure
that segments B and F of the right hand digit
light.
Lid Lock Test
If the Lid Lock Assembly or Lid Latch has been
Switch the instrument off and back on again to
serviced, carry out the following test:
exit from Test Mode 1.
Close the Lid and press [START] to start a run.
Acceptable Out-of-Balance
Try to open the Lid. The Lid should be locked
Fit 1 Cytoclip with a glass slide and funnel and
and the Motor should continue to run.
another with only a funnel (no slide). Load these
Cytoclips into a Sealed Head in opposite Stop the run and repeat 6 times.
positions (for example positions 1 and 7). The
difference between the two Cytoclips will give Processor And Interface PCB
approximately 5g Out-of-Balance which is
acceptable.
Test
If the Processor and Interface PCB has been
Check that the instrument operates correctly up
replaced or the software has been updated,
to 2000 rpm on low acceleration. No Error
carry out the following test:
Codes should be displayed.
Unacceptable Out-of-Balance Check that all the connections to the Processor
and Interface PCB are in place.
Load a Sealed Head with 1 empty Cytoclip.
Check the links:
Start the instrument and check that the
• LK1 Not Fitted
instrument stops due to an Out-of-Balance error
(Err 03). • LK2 Not fitted
Carry out a System Reset - switch the
If the instrument does not pass the tests, re-
instrument on while pressing [STOP] at the
check the Out-of-Balance switch settings.
same time. This will clear all programs. The left
The horizontal Out-of-Balance switch hand digit of the Speed display will show '8'
typically detects over the speed while all the other displays are blank.
range 350-500 rpm
When [STOP] is released, check that the Speed
The vertical Out-of-Balance switch display shows '8888'.
typically detects over the speed Check that all LEDs are ON, and that:
range 1200-1450 rpm
• Program display 8
• Speed display 8888

Cytospin 4 Service Manual Issue 5 19

• Time display 88 After approximately 1 second, the displays
After approximately 1 second, the displays should change to:
should change to: • Program display 0
• Program display 0 • Speed display 0000
• Speed display 0000 • Time display nn
• Time display nn where 'nn' is the software version number.
where 'nn' is the software version number. After approximately 1 second,
After approximately 1 second, [MED.] should be EITHER - the last program used will be
lit, [LID OPEN] LED should be flashing and the displayed, OR [MED.] will be lit, [LID OPEN] LED
displays should again change to: will be flashing and the displays will again
• Program display 1 change to:
• Speed display 1000 • Program display 1
• Time display 01 • Speed display 1000
Open and close the Lid and check that the [LID • Time display 01
OPEN] LED turns OFF.
Use the PROGRAM [ ] and [ ] buttons to
Fit a Sealed Head, close the Lid and press check that the Program numbers change.
[START]. Check the following sequence of
Use the SPEED [ ] and [ ] buttons to check
events:
that the Speed increases and decreases
The Lid locks correctly. If the [ ] button is pressed once, the
speed should increase by 10 rpm; if the [ ]
The instrument accelerates to 1000 rpm w ithin
button is held down, the speed will increase by
15 seconds,
50 rpm. When the [ ] button is pressed, the
• 1000 rpm is held for 1 minute speed will decrease in a similar manner.
• The instrument decelerates to 0 rpm Use the TIME [ ] and [ ] buttons to check that
• The Lid unlocks the Time increases and decreases correctly. If
Check the Out-of-Balance switches operate the [ ] button is pressed once, the time should
correctly, refer to Out-of- Balance Tests. increase by 1 minute; if the [ ] button is held
down, the time will increase by 10 minutes.
Display PCB And Keyboard When the [ ] button is pressed, the time will
decrease in a similar manner.
Test
Press each Acceleration button in turn - [LOW],
If the Display PCB and/or Keyboard have been [MED.] and [HIGH].
replaced, carry out the following test:
Check that each LED lights when the button is
Check that all the connections to the Lid Lock pressed, and then switches off when the next
Assembly and Processor and Interface PCB are button is pressed.
in place.
Select an unused program. Change the Speed.
Switch the instrument ON. Check that the [SAVE PROGRAM] LED flashes.
Check that all LEDs are ON, and that: Press [SAVE PROGRAM] and check that the
LED switches off.
• Program display 8
• Speed display 8888 Open the Lid and check that the [OPEN LID]
LED is on. Close the Lid and check that the
• Time display 88
[OPEN LID] LED switches off.

Cytospin 4 Service Manual Issue 5 20

Press [START]. Check that the [LID LOCK] LED
is on.
Press [STOP]. Check that the [LID LO CK] LED
switches off when the speed is below 20 rpm.
Press [STANDBY]. Check that the LED is on,
and that all other LEDs and displays are off.
Press [STANDBY] again. Check that the LED is
off, and that all other displays revert to normal.

Cytospin 4 Service Manual Issue 5 21

Chapter 5 - Troubleshooting
General
Correct service and maintenance is essential for the long term serviceability of precision engineered
products such as Cytospin 4. We strongly recommend that an Epredia Service Contract is used to
ensure future reliability, and consistency of performance.
Table 1 shows remedial action to be taken if Cytospin fails to operate.
Table 2 details the error codes and the actions required to clear the errors.
Tables 3 to 6 relate to processing problems and suggested solutions with respect to the preparation of
cells by cytocentrifugation.
The flowcharts show the remedial action to be taken if the Cytospin 4 does not operate correctly.

Be aware of samples used. they may pose a bio hazard

Table 1 - Instrument Function

Symptom Cause Remedy

No power supply Check the mains supply

Fuse blown in plug Replace the mains fuse

Replace the instrument fuse

Displays not lit on control panel Note

Instrument fuse blown
Only a technically competent person
should replace fuses

Instrument is in standby
Press [ ]
mode – red LED is on

Stopped at instrument Press [START]

Check ranges
Programs do not run Incorrect programming 200 -2000 r.p.m.
1 - 99 minutes

Lid not closed Close lid

Table 2 -Error Codes
Audible warning tones will sound and error codes will be displayed if the Cytospin 4 detects a situation
that prevents it from working safely and efficiently.
The speed and time windows will display Err 0X where X is the error number detailed in Table 2. For
example, Error 3 would be displayed -

To clear an error, press [STOP] to silence the alarm. When the sealed head has stopped spinning, press
[STOP] again to clear the error. When an error has been cleared, the lid of the instrument must be
opened and closed.
If an error code is displayed that is not listed in Table 2, turn the instrument off and then on again to clear
them.
When an error has been cleared, the lid of the instrument must be opened and closed.
If any error does not clear, follow the appropriate flow chart.

Symptom Cause Remedy

Err 01 Lid Error - the lid switch has not • Press [STOP], then open and close
operated correctly the lid
Err 02 Speed Error - the Cytospin 4 cannot • Press [STOP], then open and close
currently maintain the normal the lid
tolerance of ±20 rpm, or has been • Check that the Sealed Head is
unable to reach the programmed installed correctly and turns freely
speed within 1 minute
Err 03 Out of Balance Error - the Cytospin • Turn the Cytospin off and then on
detects that there is a balance error again, then open and close the lid
• Check cytofunnels are evenly
distributed
• Check Sealed Head for damage
• Check that the Sealed Head turns
freely
Err 04 No longer used
Err 05 Lid Lock released manually during • Press [STOP], then open and close
run or the lid solenoid has not the lid
operated correctly
Err 06 Lid was not opened and closed Press [STOP], then open and close
when the power switched on or after the lid
an error
Table 3 - Unsatisfactory Cytocentrifugation Results – Quality

Problem Cause Solution

Before Cytocentrifugation

Poorly preserved cells Preserved poorly in vivo Request repeat specimen

Lengthy delays between collection Minimize delays (e.g. less than 4

and preparation hours. Refrigerate if longer)

Cells suspended in normal saline Use balanced electrolyte solution

Cells small in diameter; Cells collected in high proportion of Collect unfixed fresh specimens, or
optically dense mix with equal volume of 50% ethanol
strong alcohol

Alcohol added to sample chamber Add less alcohol; add alcohol carefully
begins to rise through the cell
suspension and causes cell
shrinkage when it mixes

RBCs haemolysed; Alcohol was mixed with cell Add alcohol carefully to sample
ghosts remain suspension chamber

During Cytocentrifugation

Cells air dried Cell suspension medium absorbed Fill cylindrical sample chamber
completely by filter card before cytocentrifugation; us e

Cytospin Collection Fluid; reduce the

cytocentrifugation time and / or speed

Cells air dried around Cell suspension medium almost Increase specimen volume up to
periphery of collection completely absorbed by filter card 0.5ml; use Cytospin Collection Fluid;
area reduce cytocentrifugation time and/or
speed

After Cytocentrifugation

Cells air dried Film of liquid over the cells allowed Move quickly to avoid evaporation of
to evaporate during brief interval protective film from over the cells
between unloading and immersion in
alcohol

Cells air dried around Film of liquid is thinnest at its edges Immerse cells in alcohol before air
periphery of collection and evaporates before the thick
drying progresses on to periphery of
area central area
cell area

Disrupted air dried pale Fragile cells air dried and Request repeat specimen. Do not
cells, resemble 'basket exaggerated by centrifugal force allow to air dry
cells' of haematology

Cytospin 4 Service Manual Issue 5 24

Table 4 - Unsatisfactory Cytocentrifugation Results - Quantity (number of cells)

Problem Cause Solution

Before Cytocentrifugation

Exit port blocked by filter card (TPX

No cells Request repeat specimen
only)
Abnormal cells, usually larger and
Abnormal cells in heavier than normal cells, sediment Apply centrifuge tube with cell
specimen but not on to bottom of concentrate. May be concentrate and several ml of
cytocentrifuged missed if not resuspended balanced electrolyte solution to vortex
preparations completely following conventional mixer and completely re-suspend cells
centrifugation
Enrich by conventional centrifugation.
Resuspend cells in 1-2ml balanced
electrolyte solution. Combine contents
of multiple centrifuge tubes of same
specimen when possible.
Too few cells in raw specimen
microscopically examine drop of
resuspended cell concentrate;
Base sample size on cell count.
Request repeat specimen; suggest
ways to increase cellular harvest

Too few cells added to sample Base sample size on cell count of
chamber drop of resuspended cell concentrate
Sparsely populated specimen may
have filled the cylindrical and conical Enrich specimen as described above
portions of the sample chamber
Too few cells
Partially filled cone raised level of Do not allow distal boundary of
specimen in cylinder to filter card specimen to touch filter card before
level where cells can be absorbed cyto-centrifugation

Cells crowded out by precipitated Dissolve hyaluronic acid precipitate

hyaluronic acid in joint fluid with pinch of hyaluronidase

Mix several drops of glacial acetic acid

Cells crowded out by precipitated to lower the pH and re-dissolve the
phosphate salts in urine alkaline pH dependent precipitated
phosphate salts
Use saponin to haemolyse
Cells crowded out by erythrocytes
erythrocytes
Slide is between exit port and filter Load in correct sequence; sample
card (TPX only) chamber filter card side

Cytospin 4 Service Manual Issue 5 25

 During Cytocentrifugation

Check alignment as seen through

Exit port blocked by misaligned filter
window from back of slide clip; seat
card (TPX only)
Too few cells filter card

Cells lost through gap between exit Unlikely; though check assembled unit
port and filter card for alignment

After Cytocentrifugation

Suspension medium absorbed Centrifuge specimen at 3000 r.p.m.

incompletely as a result of filter card for 10 min. to sediment cells and leave
becoming clogged by debris in debris in suspension to be discarded
previously non-centrifuged specimen with supernatant. Do not wet blotter
and / or pores collapse from too before cytocentrifugation.
much pressure from spring or Cytocentrifuge specimen at 1000 rpm
Too few cells excessive centrifugal force for 6-10 min

Unload horizontal sample chamber,

cell side up. Allow blotter to absorb
Unabsorbed suspension medium
excess liquid. Lift chamber and blotter
can induce cell wash-off
away from slide. Lay slide flat until a
thin film remains; immerse in fixative

Cytospin 4 Service Manual Issue 5 26

Table 5 - Unusual Pattern of Cell Population Distribution
Problem Cause Solution

Before Cytocentrifugation

Crescent shaped
Cylinder filled incompletely Fill cylinder completely
distribution

Cells settle in cylinder if prolonged Rapidly load sample chambers and

delay before cytocentrifugation begin cytocentrifugation immediately

Display area displaced Slide not seated to foot of

Seat slide to foot of Cytofunnel
from label end Cytofunnel

Display area displaced Slide placed in Cytofunnel label end

Insert slide label end up
towards label end down

Cells on underside of
Slides loaded backwards Orient slide with label facing exit port
slide

After Cytocentrifugation

Thinly layered cells too wet and

Cell population
are either pushed up the slide upon Let the suspension medium evaporate
streams towards label
almost completely
end or to opposite end immersion in alcohol, or slide down
the slide following immersion

Circular band of cells, Thickly layered cells, margins nearly

acellular centre, 'Bull' s dry but centre remains very wet and Add less cell suspension
Eye' distribution promotes cell wash-off

Cytospin 4 Service Manual Issue 5 27

Table 6 – Summary
To predictably produce cytocentrifuged preparations that exhibit within a 28mm² circle a representative
sample of randomly distributed, uncrowded, monolayered, flattened cells that are well preserved and
displayed, the materials and methods that follow are recommended:

Before Cytocentrifugation

Do Do Not

Use Unfixed fresh cell suspension Use cell suspensions collected in alcohol

Saponinise bloody cell suspensions Use bloody cell suspensions

Equalize differences in cell suspension Cytospin un-processed cell suspensions

Control the number of cells Estimate number of cells

Use clean Micro slides Use frosted or albumenized micro slides

Use balanced electrolyte solution Use normal saline

Keep cell suspension from the filter card Let the cell suspension touch the filter card

Fill sample chambers with similar volumes Use significantly different volumes

During Cytocentrifugation

Do Do Not

Select a speed, time and acceleration suitable for Centrifuge too rapidly
the specimen type, for example, use lower speeds
and acceleration for fragile cells Centrifuge too briefly, or for too long

After Cytocentrifugation

Do Do Not

Keep the cells slightly wet Allow the cells to air dry unless intended

Immerse immediately the cytological preparation in Let too much liquid remain on the cells
95% ethanol for ethanol preps

Allow the cellular monolayer to air-dry before Immerse in 95% ethanol until the cell monolayers
staining when using Cytospin Collection Fluid prepared with Cytospin Collection Fluid have dried
sufficiently – unless otherwise specified

Cytospin 4 Service Manual Issue 5 28

Flowchart 1
Err 01 – Lid Error

Cytospin 4 Service Manual Issue 5 29

Flowchart 2
Err 02 – Speed Error

Cytospin 4 Service Manual Issue 5 30

Flowchart 3
Err 03 – Out of Balance Error

Cytospin 4 Service Manual Issue 5 31

Flowchart 4
Program Data Corrupted
If there is a memory corruption after turning the instrument on, follow the flow chart.
Note
Err 8 or Err 16 will occur if the system crashes while the instrument is running. If either of these errors is
displayed, press [STOP]. This will cause the instrument to reset back to the factory settings and it return
to Program 1. All user defined programs will be erased.

Cytospin 4 Service Manual Issue 5 32

Flowchart 5
Err 05 – Lid Lock Error
Flowchart 6
Err 06 – Lid Not Cycled Error
Flowchart 7
Err 07 – Overspeed Error

Cytospin 4 Service Manual Issue 5 35

Chapter 6 – Parts Replacement
This chapter gives instructions to replace Note
serviceable items in the Cytospin 4. After any Some individual items may only be available as
parts have been serviced or replaced, carry out part of a kit - details are given in the Spares List
the appropriate Functional Tests detailed in in Chapter 7.
Chapter 4.

Remove the mains connector from

the instrument before you start
work

Make sure that all earth

connections are correctly replaced

Make sure that the instrument has

been decontaminated and the
product return safety declaration
has been signed by the customer
before you start work

Tool/Material Requirements
When the Cytospin 4 is serviced or repaired,
you will require the following tools and materials
as well as the usual Service Engineer’s tool kit.
M3 Torque driver
M4 Torque driver

Torque settings
Use the following standard torque settings,
unless otherwise stated:
• M3 0.5 Nm
• M4 1.8 Nm
• M5 3 Nm
Note
Available spare parts are listed in Chapter 7.
The drawings in this Chapter only list the part
numbers that can be ordered from Epredia. If
numbers are not listed in this chapter, they are
not available as spares. 

Cytospin 4 Service Manual Issue 5 36

Plinth
To separate and re-join the case and plinth (1)
• Remove the plastic cap from the drive boss.
• Hold the drive boss and unscrew the screw by 2 turns. Lift the drive boss and give the screw head a
sharp tap to release the collet. The drive boss can then be removed.

Item Part Number Quantity Description

A78310017 1 Drive Boss Kit - comprises items 1 - 6

1 1 Drive boss

1 1 Split collet

1 1 Dome plug - black plastic

1 1 Washer 2BA brass

1 1 Screw - M5 x 10 cap head

1 1 Washer M5 spring

Cytospin 4 Service Manual Issue 5 38

To separate and re-join the case and plinth (2)
• Remove the 6 fixings from the bottom of the plinth. Carefully lift up the front of the case and release
the ribbon cable from the Processor PCB.
• Carefully lift the case away from the plinth.
• To remove the pull out reference card, carefully feed it out of the runners towards the back of the
instrument. Replace the card in the same way, from the back of the instrument.
• To re-join the case and plinth, follow steps in paragraphs 1 and 2 on page 42 in reverse order.

Item Part Number Quantity Description

1 A78310008 2 M4 x 12 SEM (hex) (part of Fastener Kit)

2 A78310008 4 Spacer - M4 male / male threaded (part of

Fastener Kit)

3 A78310008 4 Washer - M4 nylon retaining (part of

Fastener Kit)

4 A78330014 1 Pull out reference card

Cytospin 4 Service Manual Issue 5 39

To replace the moulded plinth
• To replace the moulded plinth, follow page 42 to separate the case from the plinth.
• Refer to the Plinth section to remove the components from the old plinth and replace them in the new
plinth.
• Refer to the Plinth section (in reverse order) to re-join the case to the new plinth.

Item Part Number Quantity Description

1 A78310018 1 Moulded plinth

Cytospin 4 Service Manual Issue 5 40

To replace the Out-of-Balance microswitches
• Remove the brackets that secure the microswitches to the plinth.
• Change the Out-of-Balance detect cableform.
• To set the microswitch positions, fit the drive boss and a full Sealed Head.
• Use feeler gauges to set the horizontal and vertical microswitches to activate 1.8mm away from the
frame.
• Tighten the screws that secure the Out-of-Balance sensor brackets to 0.5Nm.
• Remove the Sealed Head and drive boss.

Item Part Number Quantity Description

A78310013 1 Out-of-Balance Sensor Kit - comprises

items 1 & 2

1 2 Microswitch mounting bracket

2 1 Out-of-Balance detect cableform (includes

switches)

Cytospin 4 Service Manual Issue 5 41

To replace the motor controller
• Disconnect the connectors from the motor controller.
• Loosen the 4 screws that secure the controller to the bracket, then lift the motor controller away.
• Follow the above instructions in reverse order to refit the motor controller.

Item Part Number Quantity Description

1 AP14339 1 Motor Controller

2 A78320011 1 Motor Controller mounting bracket

3 A78320017 1 Motor Controller cableform

Cytospin 4 Service Manual Issue 5 42

To replace the motor
• Disconnect the motor connector from the motor controller.
• Cut off the cable ties that secure the motor cable to the suspension frame.
• Remove the 4 screws that secure the motor to the suspension frame. Remove the motor.
• Follow points 1 to 3 in reverse order to fit the new motor. Tighten the motor mounting screws to
1.8Nm.
Make sure the motor wires are routed through the slot in the suspension frame and are secured to
the 2 mounting points on the suspension frame and on the motor bracket.
Make sure that there is enough slack in the wires.
Make sure that the large cable tie (item 2) secures the wires close to the motor.

Item Part Number Quantity Description

A78310014 1 Motor Spares Kit - comprises items 1 & 2

1 1 Motor assembly

2 1 Large cable tie

Cytospin 4 Service Manual Issue 5 43

To replace the suspension frame
• Disconnect the motor connector from the motor controller and cut the cable tie on the motor
controller bracket.
• Release the 4 centralising springs from the frame and unscrew the 4 screws that secure the
suspension frame to the wire rope mounts (A).
• Refer to Replace the Motor section to remove the motor.
• Follow the above instructions in reverse order to refit the frame.
• Refit the motor, refer to Replacing the Motor section and set the Out-of-Balance microswitches.

Item Part Number Quantity Description

1 A78320016 1 Suspension frame

2 A78310015 4 Centralising spring (part of Centralising

Spring Kit)

3 A78310016 4 Wire rope mount (part of Wire Rope Mount

Kit)

Cytospin 4 Service Manual Issue 5 44

To replace the wire rope mounts
• To remove the wire rope mounts, undo the screws that secure the wire rope mount to the Plinth (A1)
and suspension frame (A2).

Always replace the wire rope mounts as a set of 4

• To replace the wire rope mounts, orientate the mount as shown in the illustration, and sit the base of
the wire rope mount against the protrusion on the plinth (B).
• Tighten the screws (A1 and A2) to 1Nm. Repeat steps 2 and 3 for each wire rope mount in turn.

Item Part Number Quantity Description

1 A78310016 4 Wire rope mount (part of Wire Rope Mount

Kit)

Cytospin 4 Service Manual Issue 5 45

To replace the centralising springs
• To remove the centralising springs, cut off the retaining rings (A) and then release the other end from
the suspension frame.
• To replace the centralising springs, attach one end to the suspension frame and then attach the other
end to the mounting pillar.
• Fit the retaining ring (A) to secure the spring (B).
• Set the Out-of-Balance switches, refer to Replace the Out-of-Balance microswitches section.

Item Part Number Quantity Description

1 A78310015 4 Centralising spring (part of Centralising

Spring Kit)

Cytospin 4 Service Manual Issue 5 46

To replace the Processor and Interface PCB
• Disconnect all the cableforms from the Processor and Interface PCB.
• Use pliers to gently push in the protrusions on the plastic mounting pillars (A1 - A4).
• Gently pull the Processor and Interface PCB off the mounting pillars.
• Follow the above instructions in reverse order to refit the PCB.

Item Part Number Quantity Description

1 A78310023 1 Processor and Interface PCB

Cytospin 4 Service Manual Issue 5 47

To replace the mains inlet assembly
Remove the mains connector from the instrument before you start work

• Disconnect the blue and brown wires from the mains inlet assembly and then remove the green and
yellow earth lead from the Power Supply Unit.
• Unclip the mains inlet assembly from the plinth.
• Orientate the new mains inlet assembly as shown in the illustration and fit the new mains inlet
assembly following points 1 and 2 in reverse order. Fit the brown wire to terminal A (bottom terminal)
and the blue wire to terminal D (top terminal) of the mains inlet assembly.
• Fit 2, T5A fuses to the mains inlet assembly.
Make sure that the primary earth connector (green and yellow wire) is reconnected as shown
below

Item Part Number Quantity Description

1 A78320021 1 Mains inlet to Power Supply Unit

cableform

2 77520137 1 Mains inlet assembly (no fuses)

3 A78320018 1 PSU to Processor and Interface PCB

cableform

4 A78310021 2 T5A (240V) fuse (part of Fuse Spares kit)

Cytospin 4 Service Manual Issue 5 48

To replace the Power Supply Unit
Remove the mains connector from the instrument before you start work

• Remove the 3 screws that secure the Power Supply Unit to the plinth. Disconnect the wires from the
PSU terminals.
• Follow the above instructions in reverse order to fit the new Power Supply Unit.
Make sure that the primary earth connector (green and yellow wire) is reconnected as shown
below

Item Part Number Quantity Description

1 AP14341 1 Power Supply Unit

Cytospin 4 Service Manual Issue 5 49

Case

Cytospin 4 Service Manual Issue 5 50

To replace the Display PCB
• Disconnect all connectors from the Display PCB and remove the 5
• screws that secure the PCB. 2 Fit the new Display PCB and reconnect all the cableforms (see
photograph)

Item Part Number Quantity Description

1 A78310022 1 Display PCB

2 A78310008 5 M3 x 8 SEM (pan pozi) (part of Fastener

Kit)

Cytospin 4 Service Manual Issue 5 51

To replace the keyboard
• Remove the case from the plinth.
• Disconnect the keyboard connector from the Display PCB (PL1). Gently lever the keyboard away from
the moulding.
• To replace the keyboard, degrease the area and clean away any debris.

Do not use xylene, toluene or any other similar solvent

• Insert the flexible cable through the aperture in the moulding and align the panel with the three bumps
in the moulding (2 on the lower edge of the recess and 1 on the left hand side of the recess).
• Remove the backing from the new keyboard and stick it in place.
• Reconnect the keyboard to the Display PCB.

Do not fold or damage the keyboard flexible cable

Item Part Number Quantity Description

1 A78330038 1 Keyboard

Cytospin 4 Service Manual Issue 5 52

To remove the splash guard
• Remove the plastic rivets (item 2).
• Remove the splash guard (item 1).

Item Part Number Quantity Description

A78310009 1 Lid Lock Spares Kit - includes items 1 & 2

1 1 Splash guard

2 4 Plastic snap rivet (also part of Fastener Kit

- A78310008)

Cytospin 4 Service Manual Issue 5 53

To replace the lid lock assembly
• Remove the splash guard.
• Disconnect the lid lock assembly connectors from the Display PCB (A) and unscrew the 4 screws that
hold the lock to the case.
• To replace the lid lock assembly, refit the components in the reverse order. Make sure that the pin on
the lock bracket mates with the face of the latch in the lid.
• Operate the solenoid to make sure that the solenoid shaft does not foul the hook and that it locks the
latch in place.

Item Part Number Quantity Description

1 A78310009 1 Lid lock assembly (part of Lid Lock Spares

Kit)

2 A78310008 4 M4 x 10 SEM (pan pozi) (part of Fastener

Kit)

Cytospin 4 Service Manual Issue 5 54

To change the lid lock assembly components
• Remove and replace each part as shown in the diagram.

Item Part Number Quantity Description

1 A78320013 1 Lock solenoid

2 A78320005 1 Lid lock sensor assembly - solenoid

position

3 A78310008 2 Cable tie (part of Fastener Kit)

4 1 Catch plate

5 A78310008 2 Snap-in rivets (black) (part of Fastener Kit)

6 A78310008 4 M3 8 SEM (pan pozi) (part of Fastener Kit)

7 A78310010 1 Microswitch (part of Lid Closed Spares Kit)

Cytospin 4 Service Manual Issue 5 55

To replace the bump stops
• To remove, gently lever the bump stops away from the moulding.
• To replace the bump stops, degrease the area and clean away any debris.

Do not use xylene, toluene or any other similar solvent

Item Part Number Quantity Description

1 A78330030 2 Bump stop

2 A78310004 1 Case assembly

3 A78310003 1 Lid assembly

Cytospin 4 Service Manual Issue 5 56

Lid

Cytospin 4 Service Manual Issue 5 57

To remove and replace the lid

Open the lid before you start to fit the lid to the case

• To remove the hinge pins from the lid and case Mouldings, remove the retainers and carefully ease
out the pin from the middle of the Mouldings.
• Use pliers to pull each pin from the moulding.
• To fit the new lid, work from both sides and insert the pins as far as the spring area.
• Fit the springs - make sure that the ends of the springs are fitted into the grooves in the lid.
• Fit the spacers and push in the hinge pins as far as they will go.
• Fit the 2 push on retainers.

Item Part Number Quantity Description

A78310011 1 Lid Hinge Spares Kit - comprises items 1 -

4

1 2 Hinge pin

2 2 Spring - torsion

3 2 Push on retainer

4 2 Spacer

Cytospin 4 Service Manual Issue 5 58

To replace the inner liner and seal assembly
• Pull out the 10 plastic clips and remove the liner and seal assembly.
• Replace the liner and seal assembly.
• Push in the new clips. Do not reuse the old clips.

Item Part Number Quantity Description

A78310012 1 Lid Seal Assembly Spares Kit - comprises

items 1 - 3

1 1 Inner liner

2 1 Seal

3 10 Clip

Cytospin 4 Service Manual Issue 5 59

To replace the inner liner seal
• To replace the seal, remove the old seal from the inner liner.
• Fit the seal around the liner - start at the hinge end of the liner. Make sure the seal lip faces inw ards
(see diagram).
• Press the seal into the liner.

Item Part Number Quantity Description

1 1 Inner lid

2 A78330032 1 Lid seal extrusion

Cytospin 4 Service Manual Issue 5 60

Sealed Head
To replace the Sealed Head Hub
• Fit the seal into the inner bowl and then secure the hub to the inner bowl as shown.

Item Part Number Quantity Description

1 59920046 1 Inner bowl

2 59910019 1 Seal (part of Sealed Head Spares Kit)

3 59930093 1 Sealed Head hub

4 1 Washer M3 - flat

5 1 Washer M3 - spring

6 1 Screw - M3 x 8 pan head

Cytospin 4 Service Manual Issue 5 61

To replace the Sealed Head lock assembly
• Carefully place the 3 ball bearings into the locking device body.
• Fit the Sealed Head handle assembly through the lid and assemble as shown.
Note
Do not use Loctite on screws.

Item Part Number Quantity Description

1 P07437 1 Moulded lid

74010104 1 Locking Device Kit - comprises items 2 - 6

2 1 Locking device body

3 1 Sealed Head handle assembly

4 1 Seal

5 3 Balls

6 4 Screw - M3 x 8 countersunk

Cytospin 4 Service Manual Issue 5 62

Chapter 7 – Specification,
Accessories and Spares
Specification
Physical
Width 405 mm (16 ins)

Depth 620 mm (max) (24½ ins)

Height 240 mm (9½ ins) (lid down)

625 mm (25 ins) (lid up)

Weight 12 kg (26½lbs)

Electrical

Power Supply Voltages 100 - 240 V a.c. (~); 5A 50/60 Hz 150VA

Note
Maximum supply voltage fluctuations not to exceed ±10% of nominal voltage.

Sound Power Level

< 53dB (this should not present any hazard to the user).

Fuses
Mains plug fuse (where applicable) 5A 250V

Mains input fuses (x 2) T5A, 250V

(Epredia Part number for Fuse spares kit - A78310021)

Note
Fuses should only be replaced by technically competent personnel.

Program Details
Time 1 - 99 minutes

Speed 200 - 2000 rpm (in increments of 10 rpm)

Acceleration High, Medium, Low

Cytospin 4 Service Manual Issue 5 63

Switch Convention
I Power On

O Power Off

Environment

General Indoor use only

Operating Temperature +2°C to +40°C

Transit/Storage Temperature -25°C to +55°C (+70°C for short exposure)

Humidity 80% max. for temperatures < 31°C

50% max. for temperatures 31°C to 40°C (Non-

condensing environment)

Altitude up to 2000m (6,500 feet)

Pollution degree 2

Over voltage category II

Part Numbers
Cytospin 4 A78300001 (with starter kit)

A78300002 (without starter kit)

Note
Starter kit comprises

EZ Double Cytofunnels with double Cytoslides

EZ Single Cytofunnel (White Filter Card) with single Cytoslides

EZ Megafunnels with slides

Note
Both versions of Cytospin 4 come with a Sealed Head

Cytospin 4 Service Manual Issue 5 64

Accessories
Use only Cytospin accessories from Epredia. Failure to do so may result in unsafe operation
and/or give inaccurate diagnostic information

Description Quantity Epredia Part Number

Centrifuge Accessories

Sealed Head (not including Cytofunnels) single 59910018

Other Accessories

Sample Chambers

EZ Single and Double Cytofunnels: (disposable; integral filter cards; packs include caps)

Single, white filter card pack of 50 A78710003

Single, brown filter card pack of 50 A78710004

Double, white filter card pack of 50 A78710005

EZ Megafunnel - 6ml (includes caps and slides) pack of 25 A78710001

Cytoslides:

76 x 26mm glass slides with outlined specimen area and frosted area at one end for identification

Single circle: coated; circle on back pack of 100 5991056

Single circle non-coated; circle on back pack of 100 5991051

Single circle non-coated; circle on front pack of 100 5991059

Double circle coated pack of 100 5991055

Double circle non-coated pack of 100 5991054

Megafunnel slide coated; rectangle pack of 25 5991026

Double circle blue mask pack of 100 B5991050

Single circle: black mask pack of 100 5991057

Preparation:

Cytospin Collection Fluid

10 litre bottles pack of 2 9990310

4 litre bottle with pump single 6768001

Cytospin 4 Service Manual Issue 5 65

500 ml bottles pack of 2 6768315

120 ml cups (filled to 60 ml) pack of 125 9990323

CytoRich Red Collection Fluid:

4 litre bottle single B9990800

500 ml bottles pack of 2 B9990801

120 ml cups (filled to 60 ml) pack of 125 B9990803

20 ml vials (filled to 10 ml) pack of 180 B9990802

Mucolexx Transport Fluid

4 litre Bag-In-A-Box single 9990371

500 ml bottles pack of 4 9990370

120 ml cups (filled to 60 ml) pack of 50 9990375

Cell-Fixx Spray Fixative

50 ml spray bottle pack of 6 6768326

General Spares

Service Manual single A78310251

Silicone Grease (for Drive Boss) 60g tube P01913

Standard Workflow Diagram Card single A783-1001

Water Soluble Felt Pen single A78330031

For more details, please refer to the latest Catalogue

Cytospin 4 Service Manual Issue 5 66

Spares List
Description Part number Quantity

The Plinth

Cableforms: Mains Inlet to Power Supply Unit A78320021 1

Motor Controller A78320017 1

PSU to Processor and Interface PCB A78320018 1

Centralising Spring Kit A78310015 1

Retainer fixing ring 4

Spring - extension 4

Drive Boss Spares Kit A78310017 1

Domed plug 1

Drive boss 1

Split collet 1

Spring washer - M5 1

Washer - 2BA 1

Screw - M5 x 10 cap head 1

Mains Inlet Assembly (note - no fuses included) 77520137 1

Motor Controller AP14339 1

Motor Controller Mounting Bracket A78320011 1

Motor Spares Kit A78310014 1

Cable tie 1

Motor assembly 1

Moulded Plinth A78310018 1

Out-of-Balance Sensor Kit A78310013 1

Microswitch mounting bracket 2

Nut - M2 4

Out-of Balance Detect cableform 1

Screw - SEM - M3 x 8 pan head pozi 4

Screw - M2 x 10 pan head pozi 4

Cytospin 4 Service Manual Issue 5 67

 Shakeproof washer - M2 4

Washer - M2 4

Processor and Interface PCB A78310023 1

Power Supply Unit AP14341 1

Pull Out Reference Card A78330014 1

Suspension Frame A78320016 1

Wire Rope Mount Spares Kit A78310016 1

Screw - SEM - M3 x 20 pan head pozi 4

Screw - SEM - M3 x 12 pan head pozi 4

Wire rope mount 4

The Case

Bump Stop (lid) A78330030 1

Cableforms - Display PCB Ribbon Cable A78320019 1

Case Assembly - includes A78310004 1

Plastic case

Touch panel

Display PCB

Bump stops

Manual release tube

Display PCB A78310022 1

Lid Closed Spares Kit A78310010 1

Lid Closed Sensor cableform 1

Microswitch 1

Lid Lock Sensor Assembly A78320005 1

Lid Lock Spares Kit A78310009 1

Splash guard 1

Lid lock assembly 1

Panel rivet 4

Lock Solenoid A78320013 1

Cytospin 4 Service Manual Issue 5 68

Keyboard A78330038 1

Lid Assembly - includes A78310003 1

Lid and window assembly

Inner liner and seal

Lid lock

Pivot pins

Lid Hinge Spares Kit A78310011 1

Hinge pin 2

Retainer fixing ring 2

Spacer 2

Spring - torsion 2

Lid Seal Assembly Spares Kit A78310012 1

Clip 10

Inner liner and seal 1

Lid Seal A78330032 1

Cytoclip Slide Clip 59910052 6

Inner Bowl 59920046 1

Lid Assembly 74010103 1

Locking Device Kit 74010104 1

Ball 3

Locking device body 1

Sealed Head handle assembly 1

Seal 1

Screw - M3 x 8 4

Moulded Lid P07437 1

Sealed Head Hub 59930093 1

Sealed Head Seal Kit 59910019 1

(Each Sealed Head requires 3 different seals - this pack
includes 1 of each type of seal required)

Cytospin 4 Service Manual Issue 5 69

 Cone Seal 1

Lid Seal 1

O-Ring Seal 1

Support Plate 59920047 1

Thumb Nut 59930098 1

Emergency Access Tool A78310007 1

Fastener Kit A78310008 1

Cable tie 3

Cable tie holder 8

Domed plug - manual release 2

Dowel pin 2

Foot 4

Panel rivet 4

PCB mounting pillar / washer 4

Retainer fixing ring 4

Screw - SEM - M3 x 6 pan head pozi 4

Screw - SEM - M3 x 8 pan head pozi 6

Screw - SEM - M4 x 10 pan head pozi 6

Screw - M4 x 12 hex head 2

Spacer - M4 male / female 4

Spring - compression 1

Washer - flat i/d 4.3mm; o/d 12mm 4

Fuse Spares Kit A78310021 1

T5A, 250V 6

T3.15A, 250V 3

T6.3A, 250V 2

Mains Lead

Europe P12033 1

UK P12031 1

Cytospin 4 Service Manual Issue 5 70

 US P12032 1

Operator Guide - English A78310250 1

Packaging AP14378 1

Service Extension Kit (ribbon cable) A78320022 1

Software Download Serial Lead 75220418 1

Cytospin 4 Service Manual Issue 5 71

Chapter 8 – Drawings
This section contains reference drawings that may help when servicing the instrument.

Electrical Drawings
• A783-0105 Service Schematic Diagram
• A783-0106 Display PCB Circuit Diagram
• A783-0107 Processor and Interface PCB Circuit Diagram

Cytospin 4 Service Manual Issue 5 72

Cytospin 4 Service Manual Issue 5 73
Cytospin 4 Service Manual Issue 5 74
Cytospin 4 Service Manual Issue 5 75
Cytospin 4 Service Manual Issue 5 76
Chapter 9 - Routine Service
Instructions
Annual Service
Before starting to service the instrument, carry out standard earth continuity and insulation
resistance tests. If the instrument fails these tests or does not work, do not proceed with the
service until the cause of the failure has been established and the fault corrected

Make sure that the instrument has been decontaminated and the product return safety
declaration, refer to the Routine Service Schedule, has been signed by the customer before
you start work

Earth Continuity and Insulation Resistance Tests

Carry out Earth Continuity and Insulation Resistance Tests. Earth Continuity should measure less than
0.1Ω; Insulation Resistance should measure greater than 2MΩ. Use the PSU frame or the fixing screw
underneath the instrument for the earth connection

General
Check the instrument for damage or any cracks in any part of the casework. Replace any damaged parts
to ensure safe operation. Check that the mains cable is undamaged. Replace if necessary. Check that
the Keyboard and Display operates correctly, refer to Display PCB and Keyboard Test.

Sealed Head
Check the Sealed Head for any cracks. Replace any damaged parts to ensure safe operation.
Replace the three Sealed Head seals, refer to Appendix C.

Lid Seal
Check the Lid Seal for wear or damage. Replace if necessary, refer to Replace the Inner Liner Seal.

Main Instrument
Separate the Case from the Plinth, refer to Separate and re-join the case and plinth section.
Check that the 8 wire rope mount fixings are secure.
Check that the 4 motor mounting screws are secure and are torqued to 1.8 Nm.
Check that the Motor Controller fixings are secure and are torqued to 1.8 Nm.
Check that the fixings that secure the Out-of-Balance sensor brackets are secure and torqued to 0. 5
Nm.
Re-join the Case to the Plinth and refit the Drive Boss, refer to Separate and re-join the case and plinth
section.
Check that the Lid Lock operates correctly, refer to the Lid Lock Test.

Cytospin 4 Service Manual Issue 5 77

Check the Out-of-Balance system operates correctly, refer to the Out-of-Balance Tests.
Check that the Motor operates correctly, refer to the Motor Tests.
Once the instrument has been checked and reassembled, apply a thin film of silicone grease to the Drive
Boss taper (Part number P01913), refer to Appendix C.
Record the Service in the Service Logbook

Cytospin 4 Service Manual Issue 5 78

Routine Service Schedule

Cytospin 4 Service Manual Issue 5 79

Cytospin 4 Service Manual Issue 5 80
Cytospin 4 Service Manual Issue 5 81
Appendices
Place the Operator Guide into the packing box
Appendix A Transportation and place the instrument on top of it.
Instructions
Lay the 'H' shaped packing strip onto the
instrument and the four cardboard
Introduction
strengthening pieces on top.
If you ever need to transport the Cytospin 4
cytocentrifuge, the following packaging Put the mains lead with the Pen and Manual
instructions should be followed. Release Tool into the smaller box and place this
box in the front of the main box.
Repackaging the Cytospin 4 Close and seal the box.
Note
If the Cytospin 4 (and Sealed Head) is to be
serviced or returned to Epredia, they must be
thoroughly cleaned and decontaminated, and
the Product Return Safety Declaration signed,
refer to Routine Service Schedule.
Press the O (OFF) side of the mains power
switch to switch the mains power switch off.

Disconnect the mains cable from the mains

supply and the Power Supply Unit.
Place the Sealed Head into the instrument and
fit the circular foam packing piece onto the top
of the Sealed Head lid. Close the Cytospin lid.
Make sure that the lid is secure.

Cytospin 4 Service Manual Issue 5 82

Appendix B Approved Appendix C Cleaning and
Reagent List Maintenance
Introduction General
This Section lists all the reagents that Epredia Cytospin is designed for easy maintenance and
specify can be used with the Cytospin 4 most fixed components such as the lid, the
cytocentrifuge. bowl liner, the control panel and the outer
housing are cleaned using a proprietary mild
If you want to use a reagent not included in this
detergent solution applied with a soft cloth.
list, contact your Epredia agent for advice.
All components and accessories that are likely
Reagents to become contaminated are also easily cleaned
• Industrial Methylated Spirits (IMS) (up to 5% with proprietary mild detergent solutions after
Methanol in Ethanol) decontamination. It is recommended that you
follow the suggested methods of
• Isopropanol (IPA)
decontamination in parallel with your local
• Ethanol procedures.
• Cytospin® Collection Fluid
For the continued safe and efficient operation of
• Mucolexx® Transport Fluid the instrument, it is important that regular
• CytoRich® Red Collection Fluid inspection of the instrument during the following
• Formal-fixxTM cleaning and maintenance procedures is carried
• Cytoblock® reagents out.

If hazardous material is spilt on, or

Cleaning Agents
inside the instrument, the user
• General purpose household or commercial should carry out the appropriate
detergent (diluted in accordance with the decontamination.
manufacturer’s instructions)
Cleaning or decontamination
• 10% commercial bleach (sodium
methods, other than those
hypochlorite)
recommended in the operator guide,
• Most proprietary disinfectants in common should be checked with an Epredia
laboratory use, such as Clorox®, or agent to ensure that the proposed
commercial disinfectants diluted with 0.3% method will not damage the
bicarbonate buffer at 7.0 to 8.0 pH, should equipment.
be suitable.
• (always test a small hidden area of the Always wear protective gloves when
instrument case first)  Water you clean or decontaminate the
Cytospin to protect yourself against
Do not use xylene, toluene or similar infection or the effects of chemicals
solvent.
Do not clean or decontaminate by
Do not use detergents that are methods that are not recommended
unsuitable for use on non-ferrous by Epredia
metals (for example Decon 90) on Do not use any chemicals that
the sealed head assembly interact with materials of
manufacture. If in doubt, check with
Epredia Service Department

Cytospin 4 Service Manual Issue 5 83

 Phenol and hypochlorites in strong Most proprietary disinfectants in common
solution will damage the instrument laboratory use, such as Clorox®, or commercial
and its accessories disinfectants diluted with 0.3% bicarbonate
buffer at 7.0 to 8.0 pH, should be suitable.
Do not use abrasive compounds or
Allow disinfectant to contact a contaminated
metal components to clean Cytospin
surface for at least one hour, where practicable,
or its components and accessories
to ensure decontamination.
Whenever the instrument has been cleaned,
Any accidental spillage of stains on
make sure that there is a thin film of silicone
the touch control panel should be
grease on the sides of the drive boss. The
removed by immediately wiping with
grease helps to make sure that the sealed head
a cloth and a small amount of
sits securely on the drive boss.
alcohol
If necessary, apply a very small amount of
silicone grease (Epredia part number P01913) Potentially lethal voltages above
onto the sides of the drive boss. 110V a.c. are present inside the unit.
DO not remove any access covers.

Routine Cleaning and Maintenance

The following table describes the cleaning
instructions for the different areas of the
Cytospin 4.

The following instructions are the

recommendations of Epredia. if
another cleaning method is required,
please contact Epredia.

Recommended Materials Remove the mains plug from the

supply socket before you clean the
If you use any cleaning materials not fixed components of the instrument
recommended in this Manual, please check with
Epredia Service Department first.

Always wipe up any spills

immediately in the event of a major
spillage, disconnect the instrument
from the mains supply without delay
and do not reconnect and switch on
until the instrument has been
thoroughly dried-out and checked by
a service engineer.

Do not use xylene, toluene or any

other similar solvent.

Cytospin 4 Service Manual Issue 5 84

Cytospin 4 Service Manual Issue 5 86
Cytospin 4 Service Manual Issue 5 87
Seal Replacement secure the cone to the base, then remove the
cone and lift the O-ring from its groove.
It is important that the instructions in
this chapter are followed if the seals To Fit the Sealed Head O-Ring Seal. Fit a
are to continue to form an effective replacement O-Ring Seal in its groove in the
biosafety seal cone. Make sure that the O-Ring fits correctly in
its groove then fit the cone to the base. Fit, then
Three flexible seals are fitted in the sealed head tighten, the four screws.
- the Cone Seal, an O-Ring Seal, and a Lid Seal.

All the seals are designed to withstand normal

cleaning and decontamination as part of the
To Remove the Sealed Head Lid Seal, pull the
routine maintenance of the sealed head.
old seal off the rim of the bowl.
However, the seals eventually become worn,
stretched, or degraded by the action of
chemicals over a period of time and should be
replaced annually. The three seals are available
in Sealed Head Spares Kit (Epredia part number
59910019).
To Remove the Sealed Head Cone Seal simply
pull the old seal off the cone.
To Fit the Sealed Head Cone Seal. Stretch the
replacement Cone Seal, with the thin lip
uppermost, onto the collar of the centre cone.
To Fit the Sealed Head Lid Seal. Place the
replacement Lid Seal on the rim of the bowl
then push the lip of the seal over the vertical
edge at the periphery of the bowl (see Lid Seal
Cross Section diagram). Make sure that the seal
fits uniformly around the rim.

To Remove the Sealed Head O-Ring Seal, Use

a screwdriver to undo the four screws that
Appendix D Technical Tips
and Service Bulletins
Introduction
Service Bulletins and Technical Tips are
regularly circulated from Epredia Service
Department.
Use this section to store these publications.

Cytospin 4 Service Manual Issue 5 89

Index
Mains Input and PSU Test..................................18
A Motor Test ..........................................................18
Appendice s .............................................................. 82 Out-Of-Balance Tests.........................................19
Appendix A Transportation Instructions Processor And Interface PCB Test.....................19
Appendix A Transportation Instruc tions ....... 82 Suspension System Te st ....................................19
Appendix A Transportation Instructions
Repackaging the Cytospin 4 ........................... 82
Appendix B Approved Reagent List ............. 83 G
Cleaning Agents............................................. 83 General Safety ............................................................7
Introduction.................................................. 83
Reagents ....................................................... 83
Appendix C Cleaning and Maintenance ....... 83 I
General ......................................................... 83 Introduction to Cytospin 4 ..........................................9
Recommended Materials............................... 84
Introduction to the Cytospin 4 ....................................9
Routine Cleaning and Maintenance................ 84
Operation ............................................................10
Routine Seal Replacement............................. 88
Safety ....................................................................9
Appendix D Technical Tips and Service
Bulletins......................................................... 89 Service ..................................................................9
L Appendix A Transportation Instructions IVD Intende d Use ........................................................9
Appendix A Transportation Instructions
Introduction.................................................. 82
P
Parts Replacement ...................................................36
C Case....................................................................50
Chemical Safety.......................................................... 8 Lid .......................................................................57
Company Information ................................................. 3 Plinth ...................................................................37
Contact address......................................................... 3 Sealed Head .......................................................61
contents...................................................................... 4 To change the lid lock assembly components ...55
To remove and replace the lid ............................58

D To remove the splash guard...............................53

To replace the bump stops ................................56
Drawings................................................................... 72
To replace the centralising springs .....................46
Electrical Drawings ............................................. 72
To replace the Display PCB................................51
To replace the inner line r and seal asse mbly......59
E To replace the inner line r seal .............................60

EMC Statement .......................................................... 6 To replace the keyboard.....................................52

Environment................................................................ 8 To replace the lid lock assembly ........................54

To replace the mains inlet assembly ..................48
To replace the motor ..........................................43
F To replace the motor c ontroller ..........................42
Functional Tests ....................................................... 18 To replace the moulded plinth ............................40
Display PCB And Keyboa rd Test ....................... 20 To replace the Out-of-Balance mic roswitches ...41
Lid Lock Test ...................................................... 19
 To replace the Powe r Supply Unit...................... 49 Sound Power Level .......................................63
To replace the Proce ssor and Inte rface PCB .... 47 Switch Convention ........................................64
To replace the Sealed Head Hub ....................... 61
To replace the Sealed Head lock assembly....... 62 T
To replace the suspension frame ....................... 44
Technical Description ...............................................11
To replace the wire rope mounts ....................... 45
Basic Structure ...................................................11
To sepa rate and re-join the case and plinth (1) .. 38
Case and Lid Assembly ................................11
To sepa rate and re-join the case and plinth (2) .. 39
Lid Lock Assembly ........................................12
Tool/Mate rial Requirements ............................... 36 Plinth Assembly.............................................11
Torque settings................................................... 36 Suspension Fra me and Motor ......................11
Produc tion Se rvices ................................................. 15 Electronics Assembly..........................................12
Introduction ........................................................ 15 Autofixation ...................................................14
Mode 1 - Out-of-Balance checks ...................... 15 Display PCB ..................................................13
Mode 2 - Speed Control and Acceleration ........ 17 Keyboard.......................................................13
Produc tion Se rvices ........................................... 15 Mains Input and Power Supply .....................14
Processor and Interface PCB .......................12
Software Updates............................................... 17
General ...............................................................11
Troubleshooting ........................................................22
R General ...............................................................22
Routine Se rvice Instructions..................................... 77 Flowchart 1 - Lid Error ..................................29
Annual Service .................................................... 77 Flowchart 2 - Speed Error.............................30
Earth Continuity and Insulation Resistance Flowchart 3 - Out of Balance Error ...............31
Tests ............................................................. 77 Flowchart 4 - Program Data Corrupted ........32
General ......................................................... 77 Flowchart 5 - Lid Lock Error .........................33
Lid Seal ......................................................... 77 Flowchart 6 - Lid Not C ycled Error ...............34
Main Instrument ............................................ 77 Flowchart 7 - Overspeed Error .....................35
Routine Se rvice Schedule ............................. 79 Table 1 - Instrument Function.......................22
Sealed Head ................................................. 77 Table 2 -Error Code s ....................................23
Table 3 - Unsatisfactory Cytocentrifugation
Results – Quality............................................24
S Table 4 - Unsatisfactory Cytocentrifugation
Results - Quantity (number of cells) .............25
Safety Information ...................................................... 7
Table 5 - Unusual Pattern of Cell Population
Specification, Accessories and Spares .................... 63
Distribution ....................................................27
Accessorie s ........................................................ 65
Table 6 – Summary .......................................28
Spares List.......................................................... 67
Specification ....................................................... 63
Electrical........................................................ 63
U
Environment .................................................. 64 USA Distributor ...........................................................3
Fuses ............................................................ 63
Part Numbers ............................................... 64
Physical......................................................... 63
W
Program Details ............................................ 63 Warranty Statement....................................................8
gg12
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