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Animal Biotechnology 3095

The document is a question paper for a B.Sc. (H) Zoology course, covering topics such as recombinant DNA technology, gene editing tools, and molecular diagnostics. It includes various types of questions, including explanations, definitions, and short notes on specific subjects. The paper consists of multiple sections, with a total duration of 3 hours and a maximum score of 75 marks.

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sahil1121verma
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45 views2 pages

Animal Biotechnology 3095

The document is a question paper for a B.Sc. (H) Zoology course, covering topics such as recombinant DNA technology, gene editing tools, and molecular diagnostics. It includes various types of questions, including explanations, definitions, and short notes on specific subjects. The paper consists of multiple sections, with a total duration of 3 hours and a maximum score of 75 marks.

Uploaded by

sahil1121verma
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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3095 4 [This question paper contains 4 printed pages.]

(b) Explain calcium chloride method of transformation. o'\· oC-2'-\ [\'\)Your


(5)
Sr. No. of Question Paper : 3095
6. (a) Explain how recombinant Insulin is produced using Unique Paper Code 3223790
recombinant DNA technology. (7)
Name· of the Paper
(b) Describe the use of T ALENS as a tool in gene Name of the Course B.Sc. (H) Zoology
editing. (5) Semester VI (DSE)

7. Write short notes on any three of the following : Duration : 3 Hours Maximum Marks : 7 5
(4x3=12)
(a) Molecular diagnosis of Sickle Cell Anaemia Instructions for Candidates
1. Write your Roll No. on the top immediately on receipt
(b) Production of pharmaceuticals in transgenic
of this question paper.
animals
2. Answer five questions in all.
(c) Microarray 3. Q. No. 1 is compulsory.
(d) Bacteriophage lambda

(e) Scope of Biotechnology

1. (a) Define the following: (5)

( i) Genetically modified organism

(ii) Humulin

(iii) DNA Polymerase

(iv) Gene Therapy

(v) Gene Knockout

(1000) P.T.O.

/
,,

3095 2 30.95 3

(b) Differentiate between any four of the following : (e) Explain any two of the following: (2+2)
(8) (i) Taq polymerase
(i) Adaptors and Linkers
(ii) Transformation efficiency
(ii) Y AC and MAC
(iii) Plaque hybridization

(iii) Plasmid and Ml3


2. (a) Describe type II restriction endonucleases and
(iv) Southern and Northern Blotting
explain their mode of action. (7)

( v) Transformation and Transfection


(b) Describe colony hybridization technique for the

( c) Expand the following : (5) screening of genomic library. (5)

(i) BAC
3. (a) Describe Western Blotting technique in detail with
(ii) NGS the help of appropriate diagrams. (7)

(iii) SCID (b) Define cloning vectors. Describe the essential


features of a cloning vector with suitable examples.
(iv) SCNT
(5)
(v) RFLP
4. (a) Explain the method of producing insect resistant
(d) Write the contributions of the following scientists. transgenic plants by recombinant DNA technology.
(5) (7)
(i) Ian Wilmut and Keith Campbell
(b) How was 'Dolly the sheep' created? Explain.
(ii) Alec Jeffreys
(5)
(iii) Smith, Nathan and Arber
5. (a) What is ex-vivo gene therapy? Describe in
(iv) Mario Capecchi & Oliver Smithies
detail. (7)
(v) Boliver and Rodriquez
P.T.O.

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