Subject: Biology PFY (PB) – Session 2025-26 Time: 50 Minutes
(Test-1)
Date: 29-05-2025 Total Marks: 25
Syllabus: CH. No. 05
Name: Section: Roll No.
(OBJECTIVE TYPE)
Note: Four possible answers A, B, C and D are given. The choice which you think is correct, fill
that circle in front of that question with marker or ink pen in the answer-book. Use of ink
remover, cutting or filling two or more circles will result in zero mark in that question.
Q.1: Each question has four possible answers. Choose the correct answer. (7×1=7)
1. What role does nicotinamide adenine dinucleotide play in oxidative pathways?
(A) Enzymes (B) Coenzyme (C) Prosthetic group (D) Inhibitor
2. The enzymes that act upon lipids and catalyse their breakdown:
(A) Lipases (B) Proteases (C) Nucleases (D) Amylases
3. Enzyme urease works best at pH:
(A) 1.5 (B) 10 (C) 4.6 (D) 7.0
4. The protein part of an enzyme is called:
(A) Apoenzyme (B) Coenzyme (C) Holoenzyme (D) Substrate
5. The substrate binds to the enzyme at the:
(A) Allosteric site (B) Binding site (C) Active site (D) Prosthetic site
6. The minimum energy required to start a chemical reaction is:
(A) Thermal energy (B) Kinetic energy (C) Activation energy (D) Potential energy
7. Enzyme activity drops after optimum point due to:
(A) More substrate (B) Denaturation (C) More cofactors (D) Low product level
==============================================================
SUBJECTIVE TYPE
SECTION I
Q.No.2 Write Short Answer. (5 × 2 = 10)
(i) Why some enzymes secreted in their inactive form?
(ii) Define cofactor and give example.
(iii) Elaborate induced fit model.
(iv) Describe competitive inhibitors with the help of an example.
(v) What are nucleases? Explain with the help of an example.
SECTION II
Q.No.3 Extensive Questions. (4 + 4 = 8)
(a) Compare the optimum temperatures of enzymes of human and thermophilic bacteria. (4)
(b) Describe how the concentration of enzyme effects the rate of enzyme action? (4)