200 Turnpike Rd

Southborough, MA 01772, USA

Phone: +1.508.281.6660
Fax: +1.508.281.6665
Email: [email protected]
Page 1 of 2
10/27/2015 rev1

Certificate of Analysis

Sample Identification:
Batch #: B-14782
BL ID #: 15-0596
Description: Amla Extract powder
Date Received: 10/9/2015

Results:
Analysis Result Units
ORAC against peroxyl radicals 1,062 µmole TE/gram
ORAC against hydroxyl radicals 6,919 µmole TE/gram
ORAC against peroxynitrite 715 µmole TE/gram
ORAC against super oxide anion 5,887 µmole TE/gram
ORAC against singlet oxygen 478 µmole TE/gram
ORAC against hypochlorite 679 µmole TE/gram
ORAC 6.0 (sum of above) 15,740 µmole TE/gram

* The acceptable precision of the ORAC assay is < 15% relative standard deviation.
There are five predominant reactive species found in the body: peroxyl radicals, hydroxyl radicals, peroxynitrite,
super oxide anion, singlet oxygen and hypochlorite. ORAC 6.0 provides a measure of the total antioxidant power of
a food/nutrition product against the six predominant reactive species.
The ORAC result is expressed as micromole Trolox equivalency (µmole TE) per gram.

Released on behalf of Brunswick Laboratories by

Jin Ji, Ph.D.
Chief Technology Officer

REFERENCES:
[1] Ou, B. et al., J Agric and Food Chem, 2001, 49 (10): 4619-4626.
[2] Huang, D. et al., J Agric and Food Chem, 2002, 50 (7): 1815-1821.
[3] Ou, B. et al., J Agric and Food Chem, 2002, 50 (10): 2772-2777.
[4] Zhang, L. et al., Free Radic.Bio Med, 2007, 43 (suppl. 1): S17.
[5] Dubost, N.J. et al., Food Chem, 2007, 105 (2): 727-735
[6] Zhang, L. et al., J Agric and Food Chem, 2009, 57(7): 2661-2667.
[7] Ou, B. et al., Method for assaying the antioxidant capacity of a sample. US Patent 7, 132, 296 B2.
_____________________________
The results shown in this Certificate of Analysis refer only to the sample(s) tested, unless otherwise stated. Attention is drawn to the limitation of liability,
indemnification, and jurisdictional issues. This Certificate of Analysis cannot be reproduced, except in full, without prior written permission of Brunswick
Laboratories, Inc.. Any misrepresentation, unauthorized alteration, or falsification of the content or appearance of this Certificate of Analysis is unlawful. The
Customer agrees and indemnifies Brunswick Laboratories, Inc., its officers, directors, employees, agents, (collectively referred to as Brunswick Labs) and agrees to
hold them harmless from any claims, judgments, actions, or expenses of any kind, including attorneys’ fees, in the event that Brunswick Labs takes action to correct
any such misrepresentation, alteration, forgery, or falsification, understanding that such conduct by Customer or its employees or agents damages the reputation and
therefore the business of Brunswick Labs. Such actions may include, without limitation, litigation or announcements to any component of or the entire relevant
industry of such misbehavior, as described above.

BIOANALYTICAL TESTING AND RESEARCH LABORATORIES

 200 Turnpike Rd
Southborough, MA 01772, USA
Phone: +1.508.281.6660
Fax: +1.508.281.6665
Email: [email protected]
Page 2 of 2
10/27/2015 rev1

Certificate of Analysis

Sample Identification:
Batch #: B-14782
BL ID #: 15-0596
Description: Amla Extract powder
Date Received: 10/9/2015

ORAC 6.0 Method Description:

Oxygen Radical Absorbance Capacity (ORAC) tests are among the most acknowledged methods that
measure antioxidant scavenging activity against oxygen radicals that are known to be involved in the
pathogenesis of aging and many common diseases. ORAC 6.0 consists of six types of ORAC assays that
evaluate the antioxidant capacity of a material against six primary reactive oxygen species (ROSs,
commonly called "oxygen radicals") found in humans: peroxyl radical, hydroxyl radical, superoxide
anion, singlet oxygen, peroxynitrite and hypochlorite. This is a comprehensive panel that evaluates the
antioxidant capacity of a material against oxygen radicals

The ORAC 6.0 tests are based on evaluating the capacity of an interested material to protect a probe (a
fluorescent probe or chromagen) from its damage by ROSs. In all ORAC assays, an ROS inducer is
introduced to the assay system. The ROS inducer triggers the release of a specific ROS, which would
degrade the probe and cause its emission wavelength or intensity change. When an antioxidant material
presents in the environment, the antioxidant absorbs the ROS and preserves the probe from degradation.
The degree of probe preservation indicates the antioxidant capacity of the material. Trolox is used as the
reference standard, and the results are expressed as mole Trolox equivalency per gram (or milliliter) of a
tested material.

The ORAC 6.0 values obtained from various natural and synthetic materials range widely due to the
significantly different natures of these materials including fresh produce, liquid, extract, pure compound,
cream, or oil. Select ORAC 6.0 values of benchmark materials are listed in our website to provide limited
scale. Comparisons between materials from similar categories are more informative and valuable.

_____________________________
The results shown in this Certificate of Analysis refer only to the sample(s) tested, unless otherwise stated. Attention is drawn to the limitation of liability,
indemnification, and jurisdictional issues. This Certificate of Analysis cannot be reproduced, except in full, without prior written permission of Brunswick
Laboratories, Inc.. Any misrepresentation, unauthorized alteration, or falsification of the content or appearance of this Certificate of Analysis is unlawful. The
Customer agrees and indemnifies Brunswick Laboratories, Inc., its officers, directors, employees, agents, (collectively referred to as Brunswick Labs) and agrees to
hold them harmless from any claims, judgments, actions, or expenses of any kind, including attorneys’ fees, in the event that Brunswick Labs takes action to correct
any such misrepresentation, alteration, forgery, or falsification, understanding that such conduct by Customer or its employees or agents damages the reputation and
therefore the business of Brunswick Labs. Such actions may include, without limitation, litigation or announcements to any component of or the entire relevant
industry of such misbehavior, as described above.

BIOANALYTICAL TESTING AND RESEARCH LABORATORIES