3+growth and Yield of Pechay Brassica Rapa L. With Varying Levels of Golden Snails Amino Acid
3+growth and Yield of Pechay Brassica Rapa L. With Varying Levels of Golden Snails Amino Acid
Abstract
This study was conducted to determine the growth and yield of pechay with varying
levels of golden snails amino acid (GSAA) on a farm lot within the school premises of
Alangalang Agro-Industrial School, Alangalang, Leyte. The research was laid out using
Randomized Complete Block Design (RCBD) for a single factor experiment with five (5)
treatments and three (3) replications. T1 (100% chicken manure) which serves as the control,
T2 (75% chicken manure and 25% GSAA), T3 (50% chicken manure and 50% GSAA), T4
(25% chicken manure and 75% GSAA) and T5 (100% GSAA).
Results showed that after ten (10) days from transplanting no significant differences
were observed on plant height, width of leaves, length of leaves and number of leaves. Similar
observations were noted after 20 days from transplanting. However, after 30 days from
transplanting or at harvest time, it was recorded that except for the number of leaves, pechay
applied with the 100% percent chicken manure produced the highest mean in terms of plant
height, width of leaves, and length of leaves. Significant differences were also recorded in the
yield of pechay.
These findings revealed that replacement or substituting the recommended rate of
chicken manure with varying levels or quantities of GSAA has resulted in a significant
deviation on most of the growth and yield parameters tested. The result is suggesting that
perhaps the amount of GSAA and the frequency of application be increased to compensate the
nutritional requirements of the plants and be comparable with the 100% chicken manure.
Keywords: Pechay, Golden Snails Amino Acid (GSAA), Growth and Yield, Randomized
Complete Block Design (RCBD).
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1. Introduction
Pechay (Brassica rapa L.) is an erect, biennial herb, cultivated as an annual about 15-
30 cm tall in vegetative stage. Ovate leaves are arranged spirally and spreading. The petioles
are enlarged and grow upright forming subcylindrical bundle. With proper management
practices, pechay can be grown in several localities across the country. The types and levels of
fertilizer applied to this crop is very important in its production.
It cannot be denied that many farmers rely heavily on inorganic or chemical fertilizers
as an important factor in meeting the food requirements of a growing population. This is
because these are easy to use, quickly absorbed and utilized by crops. However, these fertilizers
are believed to contribute substantially to human, animal food intoxication and environmental
instability or degradation (Masarirambi, et al.,2010).
The current practice in agriculture is basically chemical-based farming that makes a
considerable contribution to the degradation of our natural resources especially soils. Heavy
application of fertilizers has polluted surface and groundwater resources (dela Cruz, et
al.,2006). In recent years there is a growing trend to reduce the use of mineral fertilizers,
especially soil applied nutrients such as nitrogen (N), phosphorus (P) and potassium (K) and
their use had decreased by seven times. Moreover, there is also increasing demand for
organically-grown farm products. These create preconditions to recognize the importance of
foliar fertilization and the use of organic fertilizers as an alternative to meet plant nutrient
demand during the growing season (Kerin & Berova, 2003 as cited Bander, 2020).
One of the possible areas for study is the utilization of golden snails or kuhol in the
preparation of concoction that can be used as foliar fertilizer to vegetable crops like pechay.
Kuhol is considered an invasive pest to rice plants, but it can be a source or material to make
organic fertilizer. Mask and shell meat of golden snail has many contents such as vitamins,
proteins, fats, carbohydrates, lime and other nutrients that can be absorbed by our plantation so
it is suitable for the manufacture of liquid organic fertilizer called LOF (Siregar & Tulus. 2017).
The idea that liquid organic fertilizer from snails would improve crop production, that
the nutrient content in organic liquid fertilizer is quickly absorbed by the plants, that it would
improve the quality of growth in plants, and it is environmentally friendly has prompted the
researcher to explore this investigation on how golden snails’ amino acid as cheaper alternative
and more efficient organic fertilizer would affect the growth and yield of pechay.
Literature Review
Pechay (Brassica rapa L.) is an erect, biennial herb, cultivated annually about 15-30
cm tall in the vegetative stage. Ovate leaves are arranged spirally and spread. It is composed
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of enlarged petioles and grows upright, forming a sub-cylindrical bundle. Its inflorescence is a
raceme with pale yellow flower having seeds 1 mm in diameter and red to blackish brown
(BDPH 2016). The succulent petioles are often the most preferred main ingredient for soup and
stir-fried dishes using the immature but fully expanded tender leaves. Chinese cuisine uses
green petioles and leaves as garnish (Gonzales, Caraldre & Aban, 2015). Aside from that,
Pechay is a good source of nutrients-calcium, phosphorus, iron and vitamin B. and a healing
ingredient for hemoptysis and coughs (Marvin,2010).
Study conducted by Gonzales et al. (2015) showcased the benefits of using organic
fertilizer. Specifically, the response of Pechay (Brassica napus L.) to the varied levels of
compost fertilizer combining 75% Pure Garden Soil and 25% Pure Compost proved to generate
a remarkable growth and yield performance of Pechay in the leaf area and fresh weight. It has
greatly enhanced the growth and yield performance of Pechay. Similarly, a study by Natshen
and Mousa (2014) showed that the application of compost improved soil characteristics and
increased soil productivity and organic matter content. The result of the experiment confirmed
that using organic fertilizers increased crop productivity in cucumber.
Gonzales, Caralde and Aban (2015) study evaluated the growth and yield performance
of pechay with varied levels of compost as organic fertilizer and determined the effects of
organic fertilizer in terms of the plant height, number of leaves per plant, fresh weight per plant,
and leaf area. The result suggests that compost application significantly increased or influenced
the growth and development of pechay plant.
Study of Siregar et al. shows the effectivity of using Golden snail, Pomaceae
canaliculata as an organic fertilizer. This organisms were turned into liquid organic fertilizer
called (LOF) or and microorganisms local (MOL). The golden snail is obtained from a
livestock that is still alive and then washed, boiled and removed from its shell. The golden snail
meat is cut into small pieces, separated from the intestine and other visceral organs. The use of
mashed LOF can be sprayed on the surface of the soil or all parts of the plant. For fertilization
in rice plants the recommended dose of 250 ml/15 liters of water is sprayed on the rice age 10
days after planting and repeated again at interval distance of 15 days. Fertilization on the plant
recommended 200ml /15 liters of water sprayed on leaves and soil for 7 days after planting and
repeated every 7 days. This study revealed that the used of the golden snail as an organic
fertilizer is feasible.
Conceptual Framework
This study made use of the input-process-output model (Fig. 1). In this research, the
independent variable included the treatments of this research such as the following: T1=100%
chicken manure, T2=75% chicken manure and 25% of the volume of GSAA, T3=50% chicken
manure and 50% of the volume of GSAA, T4=25% chicken manure and 75% of the volume of
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This study was conducted to determine the effects of the different levels of golden snails
amino acid as foliar fertilizer on the growth and yield of pechay under Alangalang, Leyte soil
and climatic conditions. Specifically, this was conducted to answer the following questions:
1. What is the growth of pechay sprayed with varying levels of golden snails amino
acid in terms of:
a. Plant height;
b. Width of leaves;
c. Length of leaves;
d. Number of leaves?
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2. What is the yield of pechay due to varying levels of golden snails’ amino acid as
indicated by the weight of the plants?
3. Is there a significant difference on the growth of pechay sprayed with varying levels
of golden snails’ amino acid?
4. Is there significant difference on the yield of pechay sprayed with varying levels of
golden snails’ amino acid?
2. Methodology
This chapter presents and discusses the materials needed in the study, the different
methods employed and the cultural management practices in the conduct of the study.
Materials
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Research Design
This study followed the procedure for experimental research. Specifically, it employed
Randomized Complete Block Design (RCBD). As described by Gomez and Gomez (1984),
this is a type of experimental design characterized by blocks and all treatments are randomly
assigned in each block. In this study, five treatments were set up and this was replicated three
(3) times in order to ensure precision or accuracy of collected data.
Experimental Layout
This experiment was characterized by blocks to conform to the layout suitable for a
randomized complete block design (RCBD). The experimental area measured 65 sq. m. which
was divided into 3 blocks or replications. It was provided with 0.5-meter alleyway between
blocks. Each block was likewise divided into 5 plots with 0.5-meter distance as passage or
alleyway. Plots measured 2 meters x 1 meter. Pechay plants were spaced at 20 cm between
rows and 20 cm between hills. Each row comprised 10 plants or a total of 50 plants/plot.
Randomization of Treatments
The five (5) treatments of this study were randomly assigned using drawn-by-lot
technique. Small pieces of paper were labeled as T1, T2, T3, T4 and T5. These were rolled and
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placed in a container or box. Subsequently, these were drawn one at a time and represented
the treatment assignment for each plot. The same random technique was followed in the
random distribution of treatments to replications 2 and 3.
3. RESULTS AND DISCUSSION
Plant Height 20 days from transplanting. After 20 days from transplanting the
sample plants were again measured. This time the highest mean was noted in T1 with a mean
value of 21.49 cm, followed by T2 with an average height of 19.41 cm, then T3 with mean
score of 19.39 cm, T4 with a mean value of 18.79 cm and T5 with a mean height of 16.22 cm.
The analysis of variance has generated an F value of 3.32, which is lower than the
tabular value at the 5% level of significance and is indicative of a not significant difference in
the height of pechay due to varying levels of chicken manure and GSAA. Hence, the null
hypothesis is accepted implying that at 20 days from planting the varying amount of chicken
manure and GSAA failed to cause significant variations in the growth of pechay.
The F value of 1.56 for replication was not significant but indicative that experimental
errors within blocks were minimized while the variations between blocks were maximized.
Further, the coefficient of variations (cv) computed from this study was 9.32% that is indicative
that the precision of gathered data was within the acceptable values for fertilizer trials.
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Width of Leaves 10 days from transplanting. With regards to the width of leaves 10
days from transplanting, the highest mean was observed in T1 with 5.49 cm, followed by T5
with a mean height of 5.45 cm, T4 with 5.36 cm, T2 with 5.07 cm and T3 with 5.00 cm. The
F value computed was 2.59 which is lesser than the tabular F value of 3.84 at 5% level of
significance. This means that the treatment means being compared are just equal or
comparable. It can be deduced that after 10 days from transplanting, the width of the leaves of
pechay were statistically equal or comparable regardless of the quantity of chicken manure and
GSAA applied. Thus the null hypothesis stating no significant difference in the width of leaves
of pechay due to variations in the quantity of chicken manure and GSAA could not be rejected.
Width of Leaves 20 days from transplanting. After 20 days from transplanting the
highest mean was observed in T1 with a mean value of 9.27 cm, followed by T4 with an average
measurement of 8.90 cm, then T2 with mean score of 8.72 cm, T3 with a mean value of 8.66
cm and T5 with a mean height of 8.18 cm.
When the analysis of variance was computed, an F value of 3.96 which is lower than
the tabular value at the 5% level of significance was realized that is indicative of a not
significant difference in the width of leaves of pechay due to varying levels of chicken manure
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and GSAA. Hence, the null hypothesis was accepted implying that at 20 days from
transplanting the varying amount of chicken manure and GSAA did not cause significant
differences in the width of leaves of pechay.
The F value of 2.46 for replication was not significant and is indicative that
experimental errors within blocks were minimized while the variations between blocks were
maximized. Further, the coefficient of variations (cv) computed from this study was 3.93%,
suggestive that the precision of gathered data was within the acceptable values for fertilizer
trials.
Length of Leaves 10 days from transplanting. On the length of leaves 10 days from
transplanting, the highest mean was observed in T1 with 6.75 cm, followed by T2 with a mean
height of 6.46 cm, T5 with 6.30 cm, T3 with 6.07 cm and T4 with 6.04 cm. These mean values
were subjected to analysis of variance which has resulted in an F value of 2.65 which is lesser
than the tabular F value of 3.84 at 5% level of significance. This means that the treatment
means being compared are just equal or comparable. It can be deduced that after 10 days from
transplanting the length of the leaves of pechay were statistically equal or comparable
regardless of the quantity of chicken manure and GSAA applied. Thus, the null hypothesis
stating no significant difference in the length of leaves of pechay due to variations in the
quantity of chicken manure and GSAA could not be rejected.
Length of Leaves 20 days from transplanting. Twenty (20) days from transplanting
the highest mean was observed in T2 with a mean value of 11.66 cm, followed by T3 with an
average measurement of 11.63 cm, then T1 with mean score of 11.54 cm, T4 with a mean value
of 11.30 cm and T5 with a mean height of 9.98 cm.
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When the analysis of variance was computed, an F value equivalent to 2.25 which is
lower than the tabular value at the 5% level of significance was realized, which is indicative of
a not significant difference in the length of leaves of pechay due to varying levels of chicken
manure and GSAA. Hence, the null hypothesis is accepted implying that at 20 days from
transplanting the varying amount of chicken manure and GSAA did not cause significant
differences in the length of leaves of pechay.
Number of Leaves 10 days from transplanting. With regards to the number of leaves
10 days from transplanting, the highest mean was observed in T5 with 6.33, followed by T2
with a mean height of 6.13, T4 with 5.80, T1 with 5.60 and T3 with 5.57. When these mean
values were subjected to analysis of variance, this has resulted in an F value of 2.86 which is
lesser than the tabular F value of 3.84 at 5% level of significance. This means that the treatment
means being compared are just equal or comparable. It can be deduced that after 10 days from
transplanting the length of the leaves of pechay were statistically equal or comparable
regardless of the quantity of chicken manure and GSAA applied. Thus, the null hypothesis
stating no significant difference in the number of leaves of pechay due to variations in the
quantity of chicken manure and GSAA could not be rejected.
Number of Leaves 20 days from transplanting. After 20 days from transplanting the
highest mean was observed in T1 with a mean value of 8.03 cm, followed by T2 with an average
measurement of 7.74 cm, then T4 with mean score of 7.63 cm, T5 with a mean value of 7.43
cm and T3 with a mean height of 7.00 cm.
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When the analysis of variance was computed, an F value equivalent to 1.41 which is
lower than the tabular value at the 5% level of significance was realized, which is indicative of
a not significant difference in the number of leaves of pechay due to varying levels of chicken
manure and GSAA. Hence, the null hypothesis is accepted implying that at 20 days from
planting the varying amount of chicken manure and GSAA did not cause significant differences
in the number of leaves of pechay.
Number of Leaves 30 days from transplanting. After 30 days from transplanting the
highest mean was observed in T1 with a mean value of 9.28, followed by T2 with an average
number of 8.93, then T4 with mean score of 8.87, then T3 and T5 with the same average number
of leaves of 8.63.
When the analysis of variance was computed, an F value equivalent to 2.32 which is
lower than the tabular value at the 5% level of significance was realized, which is indicative of
a not significant difference in the length of leaves of pechay due to varying levels of chicken
manure and GSAA. Hence, the null hypothesis is accepted implying that at 30 days from
planting the varying amount of chicken manure and GSAA did not cause significant differences
in the number of leaves of pechay.
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Conclusions
Based from the findings of the study, the following conclusions are herein formulated.
1. The gradual replacement of the recommended rate of chicken manure with varying
levels of golden snail amino acid (GSAA) has significantly affected the growth and
yield parameters of pechay specifically on the plant height, width of leaves, length of
leaves and the final yield of the crop. These observations were noted on the 30th day
from transplanting or at harvest time.
2. It can be implied that the golden snails amino acid did not produce a comparable yield
with the 100% chicken manure. Perhaps the quantity of GSAA is not sufficient to
compensate the nutrient requirements of the plants.
3. The frequency of foliar application of GSAA could be a significant factor that affected
the growth and yield performance of the crop.
Recommendations
The findings of the study lead to the formulation of the following recommendations:
1. Though the effect of golden snails’ amino acid is not comparable to chicken manure, it
may still be used by farmers who are cultivating pechay for home consumption where
chicken manure is not available, most especially if the main focus is to produce an
organic vegetable.
2. The same set of experiment maybe pursued by adding the quantity of GSAA that can
be applied to the plants. Also, the frequency of the application or spray maybe
increased so as to compensate the nutrients needed by the plants.
3. Further research maybe conducted in different areas where soil and environmental
characteristics are different. It is likewise suggested that this study maybe conducted
in other months other than summer (April and May) wherein a very warm temperature
is evident.
ACKNOWLEDGMENT
The researcher was supported by the Department of Education, specifically the Alang-
Alang Agro-Industrial School. Also, the researcher thanks the Graduate School of Eastern
Samar State University, Salcedo Campus for the technical expertise provided to the researcher
in conducting her research study. The researcher is also thankful to her family for their care
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and affection. Above all, the researcher is thankful for the divine guidance bestowed on her as
she journeys in this research work.
Policy Note
GROWTH AND YIELD OF PECHAY (Brassica rapa L.) WITH VARYING LEVELS
OF GOLDEN SNAILS AMINO
NOTE: This study focused to determine the growth and yield of pechay (Brassica rapa L.)
with varying levels of golden snails amino acid (GSAA) on a farm lot within the school
premises of Alangalang Agro-Industrial School, Alangalang, Leyte.
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