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Bitterness Value Determination

The document outlines methods for determining the bitterness value of plant materials, primarily using quinine hydrochloride as a standard for comparison. It describes various techniques including Threshold Bitter Concentration (TBC) and the use of electronic tongues (E-tongues) to evaluate bitterness. The bitterness value is calculated based on the dilution of the plant extract that matches the TBC of quinine hydrochloride.

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Gogul Ramnath
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404 views3 pages

Bitterness Value Determination

The document outlines methods for determining the bitterness value of plant materials, primarily using quinine hydrochloride as a standard for comparison. It describes various techniques including Threshold Bitter Concentration (TBC) and the use of electronic tongues (E-tongues) to evaluate bitterness. The bitterness value is calculated based on the dilution of the plant extract that matches the TBC of quinine hydrochloride.

Uploaded by

Gogul Ramnath
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Determination of bitterness value

• The bitterness value is expressed in units equivalent to the bitterness of


solution containing 1gm of quinine chloride in 2000ml.The bitterness of plant
material measures by taste.

• Healthy male or female volunteers over the age of 18 years were screened
first for their ability to taste bitterness

• Methods for Determining Bitterness Values:

1. Quinine Hydrochloride Standard:

• The most common method involves comparing the plant extract to a series
of dilutions of quinine hydrochloride, a compound with a known bitter taste.

2. Threshold Bitter Concentration (TBC):

• The TBC is the lowest concentration of a substance that can be reliably


detected as bitter by taste.
3. Bitterness Value Calculation:

• The bitterness value is expressed as the reciprocal of the dilution of the plant
extract that yields the same TBC as a specific dilution of quinine
hydrochloride. For example, the bitterness value of a solution of 1 gram of
quinine hydrochloride in 2000 ml of water is set at 2 x 10^5 units.

4. Electronic Tongues (E-tongues):

• These instruments can mimic human taste perception and are used to
assess the bitterness of plant extracts and other complex samples.
• An electronic tongue (e-tongue) is an analytical instrument that uses a
sensor array to evaluate the taste qualities of liquids, mimicking the human
sense of taste. It's designed to analyze and distinguish between different
tastes in a manner analogous to how the human tongue identifies flavors.

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