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Cm-1046b Medio Selectivo Cromogenico P-Coliformes.e-Coli 500 G

The document outlines the product specification for Brilliance™ E. coli/Coliform Selective Medium (CM1046), detailing its typical formula, preparation instructions, and physical characteristics. It includes microbiological test results for various strains of Escherichia coli and Klebsiella pneumoniae, indicating expected colony appearances and growth outcomes. A satisfactory performance is defined as recovering positive strains at or above 70% of the control medium.

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Joseph Herrera Morales
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8 views1 page

Cm-1046b Medio Selectivo Cromogenico P-Coliformes.e-Coli 500 G

The document outlines the product specification for Brilliance™ E. coli/Coliform Selective Medium (CM1046), detailing its typical formula, preparation instructions, and physical characteristics. It includes microbiological test results for various strains of Escherichia coli and Klebsiella pneumoniae, indicating expected colony appearances and growth outcomes. A satisfactory performance is defined as recovering positive strains at or above 70% of the control medium.

Uploaded by

Joseph Herrera Morales
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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BT-SPEC-0260

Distribution: Central File Date: 09/11/10


Supersedes: 28/07/08
OXOID QUALITY ASSURANCE
PRODUCT SPECIFICATION

BRILLIANCE ™ E. COLI/COLIFORM SELECTIVE MEDIUM CM1046

Typical Formula*
Peptone grams per litre 8.0
Di-sodium hydrogen phosphate 2.2
Sodium chloride 5.0
Potassium dihydrogen phosphate 1.8
Sodium lauryl sulphate 0.1
Chromogenic mix 0.35
Agar 10.6

* adjusted as required to meet performance standards

Directions
Suspend 28.1g in 1 litre of distilled water. With frequent agitation, bring to the boil to dissolve completely.
Cool to 50°C. Mix well and pour into sterile Petri dishes or hold at 45°C when using the pour plate
technique. DO NOT AUTOCLAVE.

Physical Characteristics
Straw, free-flowing powder
Colour on reconstitution - straw 1-2
Moisture level - less than 7%
pH 6.7 ± 0.2 at 25°C
Clarity - clear
Gel strength - firm, comparable to 10.6g/litre of agar

Microbiological Tests Using Optimum Inoculum Dilution


Control Medium: Tryptone Soya Agar

Reactions after incubation at 37°C for 24 hours

Medium is challenged with 10-100 colony-forming units

Escherichia coli ATCC® 25922 0.5-1.5mm purple colonies, indole positive


Escherichia coli ATCC® 35218 0.5-1.5mm purple colonies, indole positive
Klebsiella pneumoniae ATCC® 29665 1-2mm pink colonies, indole negative
Klebsiella pneumoniae ATCC® 13883 1-2mm pink colonies, indole negative

A satisfactory result is represented by recovery of positive strains equal to or greater than 70% of the control
medium.

Medium is challenged with 1E+04 to 1E+05 colony-forming units

Staphylococcus aureus ATCC® 25923 No growth or pinpoint to 1mm white/cream colonies


Enterococcus faecalis ATCC® 29212 No growth or pinpoint to 1mm white colonies

Negative strains are inhibited or shall produce at least a 2 log (10) reduction when compared to the control
medium.

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