Evolution Series UV-Vis Instruments

Mercury Lamp
User Guide

269-251401 Revision A October 2013

© 2013 Thermo Fisher Scientific Inc. All rights reserved.
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Contents
Chapter 1 Mercury Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Safety Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Operating Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Installation and Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

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Mercury Lamp
This mercury lamp can be used to calibrate and verify the performance of your UV-Visible
spectrophotometer.

This section covers these topics:

Contents
• Safety Considerations
• Operating Precautions
• Installation and Operation

Safety Considerations
The mercury lamp has a safety interlock to prevent accidental exposure to dangerous levels of
UV radiation during operation. After the lamp is installed, removing the lamp from the port
while the instrument is powered on will activate the interlock to block all radiation from the
lamp.

To avoid defeating the safety interlock, keep all magnets and strong magnetic fields (such as
those produced by a mass spectrometer or various medical instruments) away from the lamp
while the instrument is powered on and the lamp cable is connected.

Related Topics
Operating Precautions

Thermo Scientific Mercury Lamp User Guide 1

1 Mercury Lamp
Operating Precautions

Installation and Operation

Operating Precautions
Handle the accessory carefully:
• Do not allow moisture to leak into the accessory.
• Wipe off spilled chemicals immediately.
• Do not drop the accessory.
• Protect the accessory from mechanical shock.
• Do not touch the exposed lens near the end of the lamp shaft (fingerprints and dirt will
degrade performance.
• Store the accessory in its case.

Related Topics
Safety Considerations

Installation and Operation

Installation and Operation

Use this accessory to perform wavelength and bandwidth tests or a wavelength calibration.
These calibration tests must be run using the main detector for the spectrophotometer.

WARNING Avoid UV radiation hazard in the mercury lamp compartment. Protect eyes
and skin from exposure.

 To install the mercury lamp

1. Remove any sampling accessory from the spectrophotometer if the accessory has a
dedicated detector.
2. Plug the lamp cable into the Hg Lamp connector on the back of the spectrophotometer.
For more information, refer to the “Connectors” section of the Evolution Series User
Guide or the INSIGHT Help system.

WARNING Avoid defeating the safety interlock. Keep magnets and strong magnetic
fields away from the mercury lamp when it is installed and the instrument is running.

2 Mercury Lamp User Guide Thermo Scientific

 1 Mercury Lamp
Installation and Operation

3. Follow these instructions to run the mercury lamp Performance Verification (PV) tests or
to perform a wavelength calibration.
• To run a PV test, in INSIGHT software click Home and then click Performance
Verification. If you have a custom button configuration, click Home, select the
Classic group and then click Performance Verification.
Select the following PV tests (deselect all other tests) and then click Run:
• Wavelength Accuracy - Hg Lines
• Wavelength Reproducibility - Hg 546
For more information, refer to the “Performance Verification” section in the
Evolution Series User Guide or the INSIGHT Help system.
• To run wavelength calibration, in INSIGHT software choose System Settings >
Calibrations. In the Lamps group, under the Hg Lamp heading, click Calibrate.

Note Calibrate only if the instrument fails the Wavelength Accuracy test.

4. When prompted, lift the Mercury Lamp Access Cover and insert the lamp into the port.
Press gently until it is secure.
For more information, refer to the “Mercury Lamp Access Cover” section in the Evolution
Series User Guide or the INSIGHT Help system.

Indicator light

A blue light on the lamp handle indicates when the accessory is properly installed.
5. Click OK to start the selected operation (PV test or calibration).
The lamp requires a few minutes to warm up. When the lamp is ready, the selected
operation starts automatically. A status bar shows the progress. When the operation is
completed, a prompt appears showing the calibration or test results.
If the calibration fails, an error message appears, contact us using the information at the
beginning of this document.

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1 Mercury Lamp
Installation and Operation

If a mercury lamp PV test fails, perform the wavelength calibration and then rerun both
tests. If a test fails again, contact Technical Support.
6. To run another operation with the mercury lamp, leave the lamp in the instrument, click
OK and proceed.
7. When finished, click OK at the prompt, remove the mercury lamp from the port and
secure the access cover.
Samples can be analyzed only when the lamp is not in the port.

 To disconnect the mercury lamp

Unplug the lamp cable and place the lamp in the provided case.

Related Topics
Safety Considerations

Operating Precautions

4 Mercury Lamp User Guide Thermo Scientific