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897.02 Aceite de Algodon en Aceites y Grasas

The document outlines AOAC Official Method 897.02 for qualitatively testing the presence of cottonseed oil in oils and fats using the Halphen Test. A mixture of carbon disulfide and amyl alcohol is combined with the test sample and heated, with a characteristic red or orange-red color indicating the presence of cottonseed oil. The intensity of the color correlates with the amount of cottonseed oil present, although various factors such as heating can affect the reaction.

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11 views1 page

897.02 Aceite de Algodon en Aceites y Grasas

The document outlines AOAC Official Method 897.02 for qualitatively testing the presence of cottonseed oil in oils and fats using the Halphen Test. A mixture of carbon disulfide and amyl alcohol is combined with the test sample and heated, with a characteristic red or orange-red color indicating the presence of cottonseed oil. The intensity of the color correlates with the amount of cottonseed oil present, although various factors such as heating can affect the reaction.

Uploaded by

andreaww1981
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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41.1.

47
AOAC Official Method 897.02
Oil (Cottonseed) in Oils
and Fats
Qualitative Halphen Test
First Action 1897
Final Action

Caution: See Appendix B, safety notes on flammable solvents,


toxic solvents, and carbon disulfide.

Mix CS2 containing 1% S in solution with equal volume amyl


alcohol. Mix equal volumes of this reagent and test sample under
examination, and heat in bath of boiling saturated NaCl solution
1–2 h. Presence of as little as 1% cot tonseed oil pro duces
pronounced characteristic red or orange-red solution. Depth of color
is proportional, to certain extent, to amount of cottonseed oil
present, and comparative tests with known mixtures of cottonseed
oil give approximate amount.
Different oils react with different intensities. Oils that have been
heated to 200°–210°C [Al len (1924) “Commercial Organic
Analysis,” 5th Ed., Vol. 2, p. 177] and hydrogenated oils [Jamieson
(1943) “Vegetable Fats and Oils,” 2nd Ed.] react with greatly
diminished intensity. Heating 10 min at 250°C renders cottonseed
oil incapable of giving reaction [Abs. J. Soc. Chem. Ind. 18,
711(1899)]. Fat of animals fed on cottonseed meal or other
cottonseed products may give positive reaction by this test.
References: J. Pharm. Chim. 6th ser., 6, 390(1897).
Abs. Analyst 22, 326(1897).
Allen (1924) “Commercial Organic Analysis,” 5th
Ed., Vol. 2, p. 177.
Conn. Agric. Expt. Stn. Rep., 1900 (II), p. 143.
Nature 178, 372(1956).
Chem. Rev. 64, 497(1964).

CAS-8001-29-4 (cottonseed oil)

ã 2005 AOAC INTERNATIONAL

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