UV/VIS Excellence
UV/Vis Excellence
UV/Vis Spectrophotometer
UV5
UV7
UV5Bio
UV5Nano
FastTrack UV/Vis Spectroscopy
Speed up Your Measurements
FastTrack UV/Vis Technology
UV/VIS Excellence
Superior Performance by Design
FastTrack™ UV/Vis technology integrates robust, state-of-the-art components into a
unique spectroscopic system design. Thus, fast reliable measurements with high traceable
accuracy are possible on a compact footprint. FastTrack UV/Vis technology and One Click™
operation comprise the basis for sustainable, trustworthy performance for the fast and
simple operation of the UV/VIS Excellence line.
Measurements within seconds Excellent robustness Clean up your lab bench
FastTrack UV/Vis technology Thanks to the FastTrack UV/Vis UV/VIS Excellence spectropho-
comprises modern fiber optics technology, there are no moving tomers have a strikingly small
in combination with array detec- parts in the instrument, and thus footprint. They can be operated
tion and a Xenon flash lamp. A it reduces the risk of misalign- as standalone without the use
full spectrum scan is performed ment. This not only contributes of a connected PC, thus saving
within just 1 second. Stable lamp to accuracy, not also brings a ample benchtop space. FastTrack
intensity conditions significantly low requirement of maintenance. UV/Vis technology allows for
increase throughput, as multiple an extremely compact layout of
reference measurements are not the optical components without
needed during sample series. compromising performance.
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Light source Light guidance Sample Detector Dispersing Computing
light and display
Reflection
grating
Partially absorbed
light beam
Glass fiber
Polychromatic
light beam
Dispersed
light CPU
Entrance
slitt CCD
sensor
Terminal
Lens
Xenon discharge
lamp
Cuvette containing
sample
Compact modularity
FastTrack UV/Vis Technology
Bring the light on the fast track
• Pulsed Xenon flash lamp and CCD array detection for
full spectrum scan within 1 second.
• State-of-the-art, long-life Xenon lamp for stable, and
repeatable measurement conditions.
• Excellent signal to noise ratio thanks to optimal light
guidance and yield through quartz glass fibers
• Always ready for measurement, no warm up time
needed.
Tailor the instrument to your
needs with smart accessories
and automation devices geared
to meet individual application
demands. The easily accessible
open sample area makes cuvette
handling fast and easy without
impacting on the compact layout
of the instrument.
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Speed up Your Measurements
Fast
Optimize Your Spectroscopic Workflow
The UV/VIS Excellence instruments optimize spectroscopic workflows effectively as the
instruments are always ready for use. Fast measurements can be reliably run by auto-
matic, pre-verified workflow routines and accessories can further automate applications
efficiently. The PC Software LabX® fosters fast spectral data management and seamless
integration into a METTLER TOLEDO based instrument network.
An instrument at the ready Ready-to-use applications Increase workflow efficiency
with automation
FastTrack UV/Vis technology Spectrum scans, fixed wave- Sample loading is easily au-
guarantees that the instrument is length absorption measurements, tomated with the FillPalMini to
ready at all times. No warm up quantification with calibration pump samples safely into the
time is needed for the Xenon flash curves or kinetic analyses are flow cell. It can also be used for
lamp to reach stability, which ready to use as direct measure- sample recovery and cuvette
speeds up measuring time. As the ments: Simply enter parameters, cleaning. Multiple samples are
lamp is only used for real mea- define the preferred workflow, efficiently analyzed using the
surements, its lifetime is greatly store as a shortcut, and start the Plug & Play CuvetteChanger,
increased. measurement with one click. including the measurement of
sample series.
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Manage your spectral data quickly and securely
LabX UV/VIS PC software expands the instrument with a sophisticated graphical
editor for spectra evaluation. Data analysis and management are simplified in
one FDA 21 CFR part 11/EU annex 11 compliant software package. To optimize
and secure your workflow even better, let the task scheduler organize your mea-
surements.
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As Easy as It Gets
Simple
Simple One Click Operation
UV/VIS Excellence instruments include One Click™, an easy and intuitive way to run tasks
right from the terminal. A large, seven inch high-resolution terminal provides clear color
representation of spectra and results at a glance. The user is always securely guided
through step-by-step instructions. UV/Vis spectroscopy has never been so quick to learn
and easy to use.
Configuration and shortcuts Optimized routines and Simple operation mode
made easy unparalleled user guidance selection
One Click UV/Vis Spectroscopy – The task icon and comments Work according to your prefer-
the home screen shortcuts allow on the touchscreen inform you ences. Use either a simple direct
you to start such tasks as direct about the status of your mea- measurement or choose from
measurements or manual opera- surements. Efficient operation is pre-defined METTLER TOLEDO
tions with just one click. Irrespec- secured as each keystroke in the methods for an immediate start.
tive of which submenu you are in, various menus provides you with In both cases, the well-defined
one keystroke takes you directly requisite information. The unclut- method editor allows easy inte-
back to the home screen. With tered home screen only displays gration of automation functions
this intuitive interface, even cus- information relevant to your daily and user-defined calculations to
tomizing shortcuts is easy. work. fit advanced application work-
flows.
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Flexible dual-mode operation
LabX UV/VIS PC software allows you the option to work at the instrument's ter-
minal or at a PC. The PC does not need to be close to the instrument if bench
space is limited. The network ensures all results are securely stored in the
LabX database regardless of where the analysis was started.
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Trust Your Results
Trustworthy
Ensure and Maintain High Performance
UV/VIS Excellence instruments are made to last. FastTrack UV/Vis technology ensures
ruggedness and optical performance while precision can be verified with traceable,
certified reference substances. The correct installation, usage and maintenance of the
instrument are supported by the unique Good UV/Vis Practice (GUVP™) services,
giving you peace of mind in your daily tasks and confidence in your results.
Keeping track of accuracy, High performance, Secure measurement
automatically low maintenance quality
The fully automatic modules Exceptional ruggedness and GUVP provides comprehensive
CertiRef™ and LinSet™ allow reliability, thanks to the lack of services that include: installa-
to test the performance of the moving parts in the optical sec- tion, operational qualification,
instrument in compliance with tion, long-lasting Xenon flash performance qualification recom-
Pharmacopeia (USP or Ph. Eur.). lamp and state-of-the art optical mendation, defined maintenance
Certified reference materials fibers. Their flexible connectivity qualification with calibration, and
traceable to NIST primary stan- to printers, PC, storage devices LabX software validation. GUVP
dards are used and a report doc- and barcode scanners, make covers the entire life cycle of the
uments all results. Performance UV/VIS Excellence instruments a instrument, improving quality
verification has never been so sound, sustainable investment while reducing risks and costs.
easy, efficient and secure. for the future.
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Ready at the start
Each UV/VIS Excellence instrument is shipped ready for immediate use. Fast-
Track technology allows for an impressively simple layout of the optical system
and does not require any adjustment during installation. Simply power up the
system and measure!
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OneDrop UV/Vis Spectroscopy
Micro-volume UV/Vis Spectroscopy
Minimize Sample, Maximize Performance
UV5Nano is the micro-volume spectrophotometry specialist for life science appli-
cations. FastTrack UV/Vis technology makes this instrument a powerful, compact
stand-alone device, which is easy to operate thanks to the One Click user interface.
The automatic pathlength selection allows measurements over a large concen-
tration range with only 1 μL of sample. Just pipette and measure!
Save precious sample Measure wide concentration Double application power
and avoid errors ranges fast and ergonomic design
Micro-volume UV/Vis measure- LockPath™ enables the mea- The UV5Nano combines two
ment is the method of choice surement of wide concentration instruments in one for micro-
when measuring either small ranges from 6 to 15,000 ng/µL volume and cuvette based mea-
sample amounts or high ab- of dsDNA without further dilu- surements. Once the arm is at a
sorption samples. Only 1 μL of tions within 2 seconds per path 90 degree position, the measure-
sample is required for reliable length. The most suitable path ment surface can be easily
measurements. The pure sample length can be automatically accessed with a pipette from
is pipetted on the measuring sur- locked. The arm design prevents either the left or the right. The
face and the arm is locked auto- the sample from drying out dur- curved lid on top of the instru-
matically to a precisely defined ing measurement, significantly ment allows convenient position-
path length. Measurement errors increasing repeatability. ing of the operator's hand to
are avoided, as the sample does securely guide the pipette tip.
not need to be diluted.
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LockPath™
path length adjustment
MAN or AUTO
Secure accuracy with LockPath™
Two path lengths
Sample
of 0.1 or 1 mm LockPath ensures that the available path lengths at
drop
Mirror 0.1 and 1 mm are accurately defined. Thanks to the
Polychromatic Reflected and partially rugged patented design, path length drift is excluded.
light beam absorbed light beam
after having passed the This eliminates expensive recalibration and downtime.
sample drop twice
The arm is securely locked and cannot be opened
until the measurement is completed; measurement er-
rors are minimized and result accuracy is preserved.
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Tailored to Your Needs
Applications
Industry-Specific Applications
UV/VIS Excellence spectrophotometers perform typical direct measurements such as
fixed wavelength, spectrum scan, quantification and kinetics. In addition, pre-verified
METTLER TOLEDO methods are available for applications in pharmaceutical, chemical,
food and beverage, and biotechnology industries, amongst others. The instruments can be
integrated into automated multi-parameter systems together with other METTLER TOLEDO
analytical instruments such as titrators, density meters, and refractometers.
Pharmaceutical, Cosmetics Chemical, Environmental Food & Beverage
• Concentration determinations, e.g. • Concentration determinations, e.g. • DOBI (Deterioration of Bleach-
- Active agent content (tablets, - Anions in water (phosphate, ability Index) of palm oil
capsules, ointments, creams) silicate, nitrate) • Browning index of fruit juices
- Content uniformity studies - Metal cations • Purity of olive oil
• Identification, e.g. Alkylamines, - Surfactants • Beer color according to ASBC
Sulphonamides, Alkaloids, - UV absorbing agents in paints and EBC
Heterocycles and varnish • Enzymatic determination of
• Purity by absorption, e.g. • Identification and spectra carbohydrates (glucose in
Peptides, proteins comparison, e.g. Pesticide food)
• Kinetic studies using specified residues, Chlorophyll • Color According to EBC, ASBC,
wavelengths or full spectra • Purity by absorption, e.g. CIE L*a*b*
- Enzyme kinetics Benzene in absolute ethanol
- color according to gardner • Color according to Saybolt,
and Hess-Ives Gardner, CIE L*a*b*, APHA/Pt-
Co/Hazen, Yellowness index
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Petrochemical Life Sciences applications Multiparameter solutions
• UV absorbance of petroleum • Nucleic acid analysis: • Fully automated multi-parame-
products (ASTM D2008) concentration determination, ter measurements e.g.
• Phosphorous in gasoline 260/280 nm ratios (with - Determination of absorbance
(ASTM D3231) background correction at at specific wavelength, pH,
• Duty markers in fuel 320 nm) for nucleic acid purity and acidity of drinking water.
• Hydrogen sulfide in fuel oils • Protein analysis: - Determination of absorbance
• UV absorbance of aromatic Biuret (Modified) Lowry, at specific wavelength, pH,
compounds Bradford, Bicinchoic acid, acidity, Brix (by refractive
• Color according to Saybold, and Trinitrobenzene sulfonate index, acid corrected) of fruit
Gardner, Yellowness Index, protein quantification methods juices.
APHA/Pt-Co/Hazen • Kinetic tests for enzyme
activity determination
• Standard cuvette or microvol-
ume down to 1 µL sample size
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Comparison Table
Model Overview and Accessories
UV5 – UV7 – UV5Bio – UV5Nano
The UV/VIS Excellence line encompasses three models for cuvette measurements and
one dedicated micro-volume instrument for life science applications. Each of the models
provides unique features that are beneficial to their respective industries.
UV5 – fast and compact
The UV5 comes ready-to-use with a 7-inch touchscreen terminal and a
precision 1 cm cuvette holder. Three direct measurement types (fixed
wavelength, scan, quantification) have been pre-installed. FastTrack™
technology allows full spectrum scans from 190 – 1100 nm in 1 second.
It is available in two versions: UV5 includes the precision 1 cm cuvette
holder and the UV5 A includes the 8-position CuvetteChanger for auto-
mated UV/Vis measurements.
UV7 – Pharmacopeia compliant
The UV7 comes with four pre-defined direct measurement types and
METTLER TOLEDO methods. The method editor allows specific adaptation
of the spectroscopic workflow. The UV7 instrument performance is tested
according to the US and European Pharmacopeia concerning photometric
and wavelength accuracy/repeatability, photometric linearity, stray light
and resolution. To learn more, please visit the link:
www.mt.com/UV-VIS-Pharmacopeia
UV5Bio – the Life Science Specialist
The UV5Bio is a dedicated instrument for standard cuvette measurements
in life sciences. Its wide range of pre-defined direct measurement applica-
tions and METTLER TOLEDO methods are typically used in Life Sciences
(biotech and biopharma), e.g. concentration determination of nucleic
acids and proteins. The UV5Bio has the same specifications and delivery
scope as the UV5.
UV5Nano – the Life Science Micro-Volume Master
The UV5Nano allows micro-volume measurements with only 1 μL of
sample, as well as standard cuvette applications. LockPath™ technology
secures path length accuracy and repeatability. It comes with a wide range
of pre-defined direct measurement applications and METTLER TOLEDO
methods typically used in Life Sciences (biotech and biopharma), e.g.
nucleic acid and protein analyses.
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Feature Comparison UV/VIS Excellence Line
Feature / Parameter UV5 UV7 UV5Bio UV5Nano
Instrument Wavelength range [nm] 190 −1100 190 −1100 190 −1100 190 −1100
performance
Resolution (toluene in hexane) > 1.5 > 1.9 > 1.5 > 1.7
Wavelength accuracy* (measured with NIST2034 holmium oxide) [nm] ±0.9 ±0.5 ±0.9 ±0.9
Wavelength repeatability (measured with NIST2034 holmium oxide) [nm] < 0.15 < 0.08 < 0.15 −
Photometric accuracy* (measured with NIST935 potassium dichromate) [A] ±0.005 (≤ 1A) ±0.005 (≤ 1A) ±0.005 (≤ 1A) ±0.006 (≤ 1A)
Photometric accuracy* (measured with NIST930/1930 neutral density filter) [A] ±0.005 (≤ 1A) ±0.005 (≤ 1A) ±0.005 (≤ 1A) −
Photometric repeatability (measured with NIST935 potassium dichromate) [A] < 0.002 < 0.002 < 0.002 < 0.003
Photometric repeatability (measured with NIST930/1930 neutral density filter) [A] < 0.003 < 0.003 < 0.003 −
> 2.0 A > 2.3 A > 2.0 A > 1.7A
Stray light at 198 nm (measured with KCl)
(< 1.0% T) (< 0.5% T) (< 1.0% T) (< 2.0% T)
> 3.5 A or > 3.7A or > 3.5 A or > 3.5 A or
Stray light at 220 nm (measured with KI)
< 0.03% T < 0.02% T < 0.03% T < 0.03% T
> 3.7A or > 3.7A or > 3.7A or > 3.7A
Stray light at 340 nm (measured with NaNO2)
< 0.02% T < 0.02% T < 0.02% T (< 0.02% T)
Noise [A] < 0.002 < 0.002 < 0.002 < 0.003
Baseline flatness [A] < 0.002 < 0.002 < 0.002 < 0.003
Tested according to USP and Ph. Eur. no yes no no
Minimal scan time full range [s] 1 1 1 1
One Click UV/Vis
™
Shortcuts per user 24 24 24 24
spectroscopy
Automation Peristaltic pump FillPalMini and SPR200 • • • •
CuvetteChanger • • • −
InMotion Autosampler • • • •
™
CertiRef automatic performance verification • • • −
™
LinSet automatic linearity verification − • − −
Applications & Direct measurements 3 4 5 5
Methods
Pre-defined METTLER TOLEDO methods − 21 22 21
Method editor • • • •
Max. number of methods 50 100 50 50
Support of the following color maps: CIE L*a*b*, CIE Luv, Tristimulus (X,Y,Z),
• • • •
Chromaticity (x,y), Lab according to Hunter
Support of the following color numbers: ASBC, EBC, Gardner, Hess-Ives,
• • • •
APHA/Pt-Co/Hazen, Saybolt, Yellowness Index
Results Number of results stored in instrument 20 100 50 50
Result storage on USB stick • • • •
Result transfer via TCP on remote PC • • • •
PC software ®
LabX UV/VIS software • • • •
Languages English / German / French / Spanish / Italian / Portuguese / Russian / Chinese / Japanese • • • •
Connectivity USB devices (memory stick for result storage, barcode reader, printer) • • • •
Interfaces: Ethernet (PC, network printer), RS232-C • • • •
Terminal 7” QVGA Color TFT 800 x 480 resolution touch sensitive screen • • • •
Instrument Width x depth x height (without terminal) [mm] 208 x 255 x 228 208 x 255 x 217
dimensions
Weight incl. terminal [kg] 6.4 7.2
The data above apply to hardware version 2 and firmware 3.0.1 or later.
*Plus individual test equipment/filter tolerance.
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Accessories
Software Printer
LabX® UV/VIS PC Software USB-P25, -P56, -P58 compact printer
Full instrument control, FDA 21 CFR part and HP and EPSON protocol network
11/EU annex 11 compliance and system printer.
integration.
Performance verification Cuvettes & cuvette holder
CertiRef ™ and LinSet™ modules Cuvettes: 1 cm, 5 cm path length optical
Automated performance verification module and quartz glass, 440 µL flow cell quartz
with certified standards, compliant with glass.
Ph. Eur. or USP. Holders: 1 cm, long path length, solid
sample, tube.
Automation Data input
CuvetteChanger: Up to 8 cuvettes, Handheld barcode reader (USB).
thermostatable.
FillPalMini: Peristaltic pump, to be used
with flow cell cuvette.
InMotion: For automatic sampling.
www.mt.com/uvvis-excellence
For more information
Quality certificate. Development, production
and testing according to ISO 9001.
METTLER TOLEDO Group
Analytical Instruments
Local contact: www.mt.com/contacts Environmental management system
according to ISO 14001.
Subject to technical changes.
© 06/2025 METTLER TOLEDO. All rights reserved. “European conformity”. The CE conformity
30256091D mark provides you with the assurance that
Marketing UV/VIS / MarCom Analytical our products comply with the EU directives.
