Allegra 6 Service Manual PDF
Allegra 6 Service Manual PDF
Rev. AA
ALLEGRA 6 SERIES
SPINCHRON R
Service Manual
COPYRIGHT INFORMATION
This Service Manual is copyrighted and all rights are reserved by Beckman Instruments,
Inc. This document may not, in whole or part, be copied, photocopied, reproduced,
translated, or reduced to any electronic medium or machine-readable form without prior
consent, in writing, from Beckman Instruments, Inc.
SAFETY REMINDERS
The following pages summarize cautionary information basic to the safe operation of
this instrument. However, it is strongly recommended that the user read the entire
manual carefully before attempting to service the instrument. In addition, be sure to
heed all NOTES, CAUTIONS, and WARNINGS, which are specifically defined as
follows:
RELATED DOCUMENTS
This Service Manual does not include the Instruction Manual for this instrument. The
Instruction Manual contains instructions for proper operation of the instrument,
information regarding the limitations, specifications, precautions and hazards
associated with its use.
To obtain a copy of the Instruction Manual, please order it through any Beckman Sales
and Service Office.
IMPORTANT NOTICE
This Service Manual is designed solely for the use of Beckman Field Service
Representatives who have and training and experience in the servicing of Beckman
products. To improve product performance, Beckman may have made changes in the
product to which this Service Manual relates. Such changes may not be reflect in this
manual. Accordingly, Beckman makes no representation or warranty, either express or
implied, that the information contained in this Service Manual is complete or accurate
with respect to the particular product to which this manual pertains, and the purchaser
must assume all risks in the use of this manual for the purpose of performing service
upon such product.
Components of this instrument which are considered user serviceable are discussed in
detail in the instrument's instruction manual.
MECHANICAL SAFETY
• Always use the tools specifically recommended for the job. This not only
reduces the chances of injury, it minimizes damage to the instrument.
• After the mechanical repairs have been completed, always check that all
mounting hardware and safety shields are in place and secure. By doing so,
you will not only ensure safe operation of the instrument, but may reduce
callbacks.
As you can appreciate, all it takes is good common sense, plus good safety techniques
that are basic and easy to remember. With this is mind, you should have no trouble
servicing mechanical devices safely.
ELECTRICAL SAFETY
It pays to take a few precautions before and during servicing to avoid injury from
electrical shock. Here are a few safety tips that can help keep you on the job without a
sudden vacation:
• Be sure to disconnect the power cord before working on any high voltage
circuitry! As power switches disappear from new equipment, this precaution is
very easy to overlook.
• Read and heed ALL caution labels! They are posted for YOUR safety.
• Take care of test equipment. Be sure to use the right probes for the right job.
Measuring high voltages, for example, requires a well-insulated, high voltage
probe. Damaged probes and cables are dangerous and should not be used.
Also, be cautious around test equipment like an oscilloscope. The
oscilloscope case may become live if you connect the cable ground to a live
circuit. Avoid this dangerous situation at all times!
• Always treat cathode ray tubes (CRTs) and uncased photomultiplier tubes
(PMTs) with special care. When carrying them, wear eyeglasses and
protective gloves. If these devices are dropped or struck with a sharp object,
they can implode violently and scatter glass.
These basic tips regarding good electrical safety practices are easy to
remember. Combined with good common sense, they should keep you on the
job for a long time to come.
ELECTROSTATIC DISCHARGE
Static electricity is an electrical charge which can damage circuitry and components
Listed below are important ElectroStatic Discharge guidelines for handling electronic
components. Following these guidelines will avoid needless damage to circuit cards
and components on them.
• Keep hand creams and food away from the conductive work surfaces. If
spilled on the bench top, these materials contaminate and increase the
resistivity of the work area.
• Check the grounding connections on wrist strap and static mats. (Mat must
have proper ground.) Make certain they fit snugly before starting work with
the components and printed circuit cards.
• Do not allow anyone not grounded to touch ESD sensitive components in the
work area. To be grounded, they must be standing on the conductive floor
mat and must touch the conductive bench top mat before touching the
components or printed circuit cards.
• Do not place ESD sensitive components on work areas that are not grounded
properly.
• Do not touch the component by the pins or leads since the most damage is
done at these points by ESD. Handle the components by the cap edges or
body and the printed circuit cards by the edges.
Working with refrigeration systems requires special equipment and training. If you are
not familiar with the operational theory for refrigeration systems and the special
requirements for performing service on a system, do NOT attempt it. Use the following
precautions for working with refrigeration systems in Beckman instruments.
• Use caution when replacing a burnt out compressor. The oil in the
compressor may be very caustic or acidic. Use protective gloves.
• Charge refrigeration systems with gas from the low side only. High side
charging may damage the compressor.
• Always insure that heated parts have cooled completely before handling
them.
Safety Reminder
Centrifuges are not designed for use with materials capable of developing flammable or
explosive vapors. Such materials should not be handled or stored near the instrument.
• Turn the POWER off and disconnect the instrument from the main power
source before performing any maintenance that requires the removal of an
instrument panel.
• Do not place containers holding liquid on or near the chamber door. If they
spill, liquid may get into the instrument and damage electrical or mechanical
components.
• Under no circumstances should you try to slow or stop the rotor by hand.
ALLEGRA 6 SERIES
& SPINCHRON R
INTRODUCTION 1-1
INSTALLATION 2-1
SCHEMATICS 4-1
PARTS 5-1
APPLICATIONS 7-1
SECTION 1
INTRODUCTION
Only values with tolerances or limits are guaranteed data. Values without tolerances
are informative data, without guarantee.
Speed
Set (in 200-rpm increments) 0 to 8000 rpm
Display ±30 rpm of actual
Set time
Timed run up to 30 min
Continuous run HOLD ( )
Temperature
Temperature setting
(refrigerated models only) not applicable
Normal operating range
(refrigerated models only) not applicable
Temperature display
(refrigerated models only) not applicable
Ambient temperature range 10 to 35°C
Ambient temperature range
for optimum operation 10 to 25°C
Humidity restrictions <95% (noncondensing)
Dimensions
Width 54.6 cm (21.5 in.)
Depth (overall) 66.0 cm (26.0 in.)
Depth (at base) 58 cm (23 in.)
Height, door closed 40.6 cm (16.0 in.)
Height, door open 96.8 cm (38.1 in.)
Weight 51.7kg (114.0 lb)
Ventilation clearances
(sides and rear) 7.6 cm (3.0 in.)
Only values with tolerances or limits are guaranteed data. Values without tolerances
are informative data, without guarantee.
Speed
Set (in 200-rpm increments) 0 to 8000 rpm
Display ±30 rpm of actual
Set time
Timed run up to 30 min
Continuous run HOLD ( )
Temperature
Temperature setting
(refrigerated models only) –10 to 40°C
Normal operating range
(refrigerated models only) –5 to 25°C
Temperature display
(refrigerated models only) actual chamber temp ±2°C
Ambient temperature range 10 to 35°C
Ambient temperature range
for optimum operation 10 to 25°C
Humidity restrictions <95% (noncondensing)
Dimensions
Width 76 cm (30 in.)
Depth (overall) 66 cm (26 in.)
Depth (at base) 58.4 cm (23.0 in.)
Height, door closed 39.4 cm (15.5 in.)
Height, door open 94 cm (37 in.)
Weight 75.8 kg (167 lb)
Ventilation clearances
(sides and rear) 7.6 cm (3.0 in.)
Only values with tolerances or limits are guaranteed data. Values without tolerances
are informative data, without guarantee.
Speed
Set (in 200-rpm increments) 0 to 8000 rpm
Display ± 30 rpm of actual
Set time
Timed run up to 30 min
Continuous run HOLD ( )
Temperature
Temperature setting
(refrigerated models only) –10 to 40°C
Normal operating range
(refrigerated models only) –5 to 25°C
Temperature display
(refrigerated models only) actual chamber temp ±2°C
Ambient temperature range 10 to 35°C
Ambient temperature range
for optimum operation 10 to 25°C
Humidity restrictions <95% (noncondensing)
Dimensions
Width 54.6 cm (21.5 in.)
Depth (overall) 66.0 cm (26.0 in.)
Depth (at base) 58.4 cm (23.0 in.)
Height, door closed 67.3 cm (26.5 in.)
Height, door open 125.7 cm (49.5 in.)
Weight 90.3 cm (199.0 in.)
Ventilation clearances
(sides and rear) 7.6 cm (3.0 in.)
The Beckman Allegra 6 centrifuge generates centrifugal forces required for a wide
variety of applications. Together with the Beckman rotors designed for use in this
centrifuge.
Available Rotors
Refer to the appropriate rotor manuals for complete rotor descriptions.
Rotor Profile and Description Max Max RCF Max Rotor Manual
RPM (× g) Capacity Number
GH-3.8A Horizontal (4 place) GS6-TB-017
3750 3210 4 × 750 mL
Buckets (rmax = 204 mm)
3250 1924 4 × 192 mL
Carriers (rmax = 163 mm)
GH-3.8 Horizontal (4 place) GS6-TB-003
3750 3210 4 x 750 ml
Buckets (rmax = 204 mm)
3250 1924 4 x 192 ml
Carriers (rmax = 163 mm)
Housing
The centrifuge housing is made of sheet steel over a steel chassis and is finished with
urethane paint. The control panel overlay is made of coated polycarbonate. Polyvinyl
chloride lines the interior walls of the centrifuge housing for sound and temperature
insulation.
Door
The structural steel door has a strobe port for speed verification. In nonrefrigerated
models, the door has two filtered air-intake ports for rotor cooling. An electromechanical
door lock system, together with a manual lock, prevents run initiation unless the door is
closed and latched. When a run is in progress, the door locks automatically and can not
be opened unless the power is on, the rotor is virtually stopped (spinning less than 40
rpm), and the manual lock is in the UNLOCK position. If there is a power failure, the
door lock can be manually tripped for sample recovery.
Rotor Chamber
The aluminum rotor chamber is coated with a chemical-resistant epoxy finish. The drive
shaft and neoprene rubber boot surrounding the drive shaft are visible in the chamber
bottom. A neoprene gasket around the chamber top assures sealing. (Centrifuge
gaskets have not been designed as bioseals for aerosol containment.) The thermistor is
also visible in refrigerated models.
Nonrefrigerated models only—During centrifugation, the rotor movement draws air
through the filtered air-intake ports in the door into the chamber bowl, where it circulates
around the rotor. Holes in the side of the bowl allow air to flow out to the exhaust ports
beneath the centrifuge. This air flow provides cooling for the rotor.
Drive
The direct-drive motor has permanently lubricated, sealed bearings. A tie-down screw
secures the rotor to the drive shaft. The resilient suspension ensures that loads will not
be disturbed by vibration, and prevents damage to the drive shaft if an imbalance
occurs during centrifugation.
NOTE: To avoid chamber icing, refrigeration is off when the door is open.
The centrifuge door must be closed and latched for the
refrigeration system to begin operating.
Safety Features
Safety features include the following:
• The door has an electromechanical door-locking mechanism and a secondary
(manual) lock to prevent operator contact with spinning rotors. When the door is
closed it locks automatically. It can be unlocked only by pressing the DOOR switch to
OPEN, and opened only when the power is on, the rotor is at rest, and the manual
lock is in the UNLOCK position. Two independent monitoring systems prevent the
door from opening if the rotor is spinning.
• A steel barrier ring surrounds the aluminum rotor chamber to provide full operator
protection.
• An imbalance detector monitors the rotor during the run, causing automatic shutdown
if rotor loads are severely out of balance. At low speeds, an incorrectly loaded rotor
can cause imbalance. Rotor instability can also occur if the centrifuge is moved while
running, or if it is not resting on a level surface.
• An anchoring system is provided to secure the centrifuge in place. The anchoring
system (bolts that anchor a benchtop centrifuge to the laboratory bench; a stationary
tray to roll a kneewell centrifuge into for operation) is designed to minimize the
possibility of injury or damage that could result from centrifuge movement in the
event of a major rotor mishap.
CFC-Free Centrifugation
To ensure minimal environmental impact, no CFCs are used in the manufacture or
operation of Allegra 6 series and Spinchron series centrifuges.
SECTION 2
INSTALLATION
Electrical Safety
To reduce the risk of electrical shock, this equipment uses a three-
wire electrical cord and plug to connect the centrifuge to earth-
ground. To preserve this safety feature:
• Make sure that the matching wall outlet receptacle is properly wired
and earth-grounded. Check that the line voltage agrees with the
voltage listed on the name-rating plate affixed to the centrifuge.
• Never use a three-to-two wire plug adapter.
• Never use a two-wire extension cord or a two-wire non-grounding type
of multiple-outlet receptacle strip.
Do not place containers holding liquid on or near the chamber door. If
they spill, liquid may get into the centrifuge and damage electrical or
mechanical components.
WARNING:
This centrifuge weighs 51.7 kg/114.0 lb (nonrefrigerated benchtop),
75.8 kg/167 lb (refrigerated benchtop), or 90.3 kg/199.0 lb
(refrigerated kneewell). DO NOT attempt to lift or move it without
assistance from This centrifuge weighs 51.7 kg/114.0 lb another
person.
WARNING:
Do not place the centrifuge near areas containing flammabl fluids.
Vapors from these materials could enter the centrifuge’s air system
and be ignited. Never bring any flammable substances within the 30-
cm (1-ft) area surrounding the a clearance envelope of 7.6 cm (3 in.)
around an operating centrifuge. No persons should be within this
clearance envelope while the centrifuge is operating.
Carefully remove the centrifuge and accessories from the shipping container. Save the
container and packing materials for possible future relocation or storage of the
centrifuge.
• Position the centrifuge on a level surface (a sturdy table or laboratory bench for
benchtop models) that can support the weight of the centrifuge (refer to
SPECIFICATIONS in Section 1) and resist vibration.
• Allow a 7.6-cm (3-in.) clearance at each side and the back of the centrifuge to
ensure sufficient air circulation. Dimensions are shown in Figure 2-1.
• Ambient temperature during operation should not exceed 35°C (95°F). Relative
humidity should not exceed 95% (noncondensing).
Use the appropriate anti-rotation kit to secure the centrifuge to the working surface. The
anti-rotation kit is designed to minimize the possibility of injury or damage that could
result from centrifuge movement in the event of a rotor mishap.
• The anchoring kit for a benchtop centrifuge contains bolts to secure the
centrifuge to the table or bench. Complete instructions (SP-TB-007) for installing the
anti-rotation kit are included with the kit, which is shipped with the centrifuge.
• The anchoring kit for a kneewell centrifuge contains a stationary tray to roll the
centrifuge into for operation. Complete instructions (GS6-TB-007) for installing
the anti-rotation kit are included with the kit, which is shipped with the centrifuge.
Electrical Requirements
A 1.8-m (6-ft) power cord with grounded plug is supplied with the centrifuge. In regions
where a different plug is required to meet local electrical and safety requirements,
contact your local Beckman representative.
Make sure the voltage imprinted on the name rating plate affixed to the back of the
centrifuge agrees with the line voltage of the outlet used. With the POWER switch in
the OFF (O) position, plug in both ends of the centrifuge power cord. If there is any
question about voltage, have a qualified service person measure it under load while the
drive is operating.
To reduce the risk of electrical shock, this equipment uses a
three-wire electrical cord and plug to connect the centrifuge to earth-ground. To
preserve this safety feature:
• Make sure that the matching wall outlet receptacle is properly wired and earth-
grounded.
• Never use a three-to-two wire plug adapter.
• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-
outlet receptacle strip.
To ensure safety, the centrifuge should be wired to a remote emergency switch
(preferably outside the room where the centrifuge is housed, or adjacent to the exit from
that room), in order to disconnect the centrifuge from the main power source in case of
a malfunction.
NOTE:
During transport between areas with varying temperatures, condensation may occur
inside the centrifuge. Allow sufficient drying time before running the centrifuge.
Explain:
Rotors
Warranty
Maintenance
Responsibility
Cautions
Descriptions of instrument
Functions
Controls
Indicators
Rotor chamber
Temperatures sensing and control
Drive
Safety features
Name rating plate, serial number
Explain:
Troubleshooting
Customer maintenance items
Beckman Coulter Field Service, service agreements, etc.
Accessories and options that are available for the instrument
How to get applications support from Beckman Coulter
SECTION 3
SERVICE PROCEDURES
WARNING:
• Maintenance should be performed only by trained, qualified personnel.
• Turn the power off and disconnect the instrument from main power prior to
performing service.
• Refrigerant gas may cause drowsiness and will displace oxygen in an enclosed
area. Avoid inhalation of this gas.
• Do not place hands or fingers in the instrument while instrument is in operation.
• Use only Beckman or Beckman approved replacement parts.
A. Open lid, TURN POWER SWITCH OFF AND DISCONNECT POWER CORD.
B. Remove the front panel perimeter hardware (do not remove latch mounting screws).
Benchtop (non-refrigerated) 10 Allen screws and 4 hex screws (bottom side).
Benchtop (refrigerated) 10 Allen screws and 5 hex screws (bottom side).
Kneewell: 10 Allen screws and 4 hex screws (bottom side).
NOTE: Prior to removing bowl, remove the black Allen head screw, located inside
the bowl on the left front side.
B. Remove bowl including drive bellows (boot) and door gasket by pulling up. The
rotor torquing bar may be used to pull the bowl upward by inserting the bar into one
of the air flow holes.
NOTE: During re-assembly insure the black Allen head screw in the bowl is
aligned with the arrow on top of housing.
B. Removed large piece of foam from behind the front panel and disconnect motor
harness connectors J/P 1,9 and 12.
C. Remove the six symmetrically spaced motor mounting lock-nuts, washers and
grommets.
D. Pull drive upward, including the blue support ring, while feeding harness through
motor insulating foam.
E. To reinstall drive, place drive on triangular plate, feed harness through the drive
opening then through the slit in the motor insulating foam and up over the ballast
ring. Carefully set drive down over the screws and ensure harness is not pinched.
Lightly coat the six new grommets with vacuum grease. Place grommets over drive
mounting screws followed by flat washers. Tighten the six lock-nuts until they are
flush with top of screws. Reconnect harness connectors J/P 1,9 and 12.
NOTE: The boot must remain completely seated down around the motor cover to
prevent contact with the rotor.
D. Pull drive upward, including the blue support ring, while feeding harness through
motor insulating foam.
screws and ensure harness is not pinched. Lightly coat the six new grommets with
vacuum grease. Place grommets over motor mounting screws followed by flat
washers. Tighten the six lock-nuts until they are flush with top of screws.
Reconnect harness connectors J/P 1,9 and 12.
NOTE: The boot must remain completely seated down around the motor cover to
prevent contact with the rotor.
B. When removing the brushes for inspection, mark each one so they can be
reinstalled in the same orientation if they are not being replaced.
C. The brushes used in the Allegra 6 motor are called Auto-Stop. A wooden dowel will
pop out when the brush wares down to ½ the length of the brush. Replace brushes
if either one is less than 1/2 inch, (13mm) in length.
NOTE: To extend the life of replacement brushes, the new set must be seated prior
to operating the drive at maximum RPM. Ensure commutator is in good condition.
E. Seat new brushes by operating the centrifuge (with rotor only), at 2000 RPM for 4
hours.
C. Remove the hex screws that fasten housing to chassis. This would include the
(5/16”) screws located around the outside edge of the rear chassis and underneath
the chassis on both sides.
B. While supporting the door, remove the (8) hex screws which hold hinges to rear
chassis.
E. Adjust striker (door locking eye bolt) to maintain a good seal between the door and
the bowl gasket (visual observation).
Warning: Refer to the safety notices in the front of this manual for cautionary notes
when working with refrigeration system. Also, heed all brazing safety
notes. Always recover the refrigerant.
B. To pre-cool rotor and chamber, set temperature knob to -2° C, speed knob to 2000
RPM and timer for 30 minutes.
C. After pre-cool, set temperature knob to -10°C and increase speed to 3750 RPM.
Set timer for 15 minutes.
D. At the instant the timer shuts off, record the indicated temperature. The reading
should be -1° C or colder for 60 Hz units and + 1° C or colder for 50 Hz units.
E. When rotor stops use a temperature meter to measure the actual temperature of
the sample (glycerol) and then record reading.
C. Before opening Schroeder valves, purge charging manifold and hoses of air with
R404a refrigerant supply at the low-side and high-side service valves.
D. To pre-cool rotor and chamber, set temperature knob to -2°C, speed knob to 2000
RPM and timer for 15 minutes.
E. After pre-cool, set temperature knob to -10°C and increase speed to maximum RPM
of rotor. Set timer to HOLD.
G. If system pressures are low, add refrigerant R404a as a gas at the low-side to
required pressure. Proceed to step 3.2.3 to check for leaks.
H. After removing refrigeration manifold, and access valves, ensure service valve caps
are tightly replaced. Insulate low-side service valve with "Presstite" insulation tape.
Figure 3-2
400
55
380
340
50
320
300
45
280
260
40 240
20 25 30 35 40
Ambient Temperature at Condenser - Degrees C
Part Number 679054
Rev. AA
50Hz Allegra 6R/KR, Spinchron R With R404A
Running a GH-3.8A Rotor at Max speed, Set Temperature at -10
400
55 320
300
50
260
240
20 25 30 35 40
Ambient Temperature at Condenser - Degreees C
Part Number 679054
Rev. AA
Part Number 679054
Rev. AA
A. Remove front panel and instrument housing as outlined in the mechanical systems
section.
C. Purge charging manifold and hoses of air before adding refrigerant to the system.
D. If system pressures are low, add refrigerant R404a as a gas to the low-side with
compressor running.
E. Turn main power off. Allow pressures to equilibrate then test for leaks. (Requires a
leak detector that will detect R404a).
H. Closed both access service valves at the compressor while the vacuum pump
continues to run. Once the valves are closed, turn off the vacuum pump and
remove the evacuation manifold.
I. Connect charging manifold and R404a refrigerant supply per procedure in step
3.2.3.
Vacuum Monitor
(338866)
Compressor
High Side
Service Valve
Evacuation Manifold
(341676)
Compressor
High Vacuum Low side
Pump Service Valve
FIGURE 3 - 3
E. Disconnect harnesses in brazing area and position them to avoid heat damage.
CAUTION: Do not damage or cut the capillary tube located inside the 1/4 inch tubing.
H. Benchtop - Observe brazing cautions. Unbraze refrigeration chamber line at low
side service valve "T". Using a tube cutter, carefully cut the 1/4 inch diameter tubing
approximately 2 inches (5 cm) from chamber foam wall.
Kneewell - Observe brazing cautions. Unbraze refrigeration chamber low-side line
approximately 8 inches (20 cm) from chamber foam wall. Using a tube cutter,
carefully cut the 1/4 inch diameter tubing approximately 8 inches from chamber.
J. Remove chamber.
K. Re-assemble the chamber, by performing the above steps in reverse order. Use a
connecting coupler to braze the 1/4 inch tubing which contains the capillary. DO
NOT CUT OR DAMAGE THE CAPILLARY TUBE. Evacuate, charge and leak test
the refrigeration system per step 3.2.3.
A. Power off, adjust temperature indicator needle to read 15°C by using meter zero
adjustment located below meter face.
C. Remove jumper (U1 TEMP), located on control bd. to the CAL position.
E. Power on, adjust R146 on control board for a reading of + 3.5°C on temperature
meter. Be patient there is a short response time delay.
G. To check temperature knob calibration turn knob above and below 3.5°C.
Compressor should turn on and off at 3.5°C +/- 1°C.
H. Turn power off and move JU1 jumper to the NORM position. Reconnect J/P21.
NOTE: Ensure chamber gasket and door form a good seal prior to performing
refrigeration tests.
Resistance versus Temperature
10 K at 25 C
Temp. C Res. Temp. C Res. Temp. C Res. Temp. C Res.
-50 441.3K 0 29.49K 20 12.26K 40 5592
-40 239.8K 1 28.15K 21 11.77K 41 5389
-30 135.2K 2 26.89K 22 11.29K 42 5193
-20 78.91K 3 25.69K 23 10.84K 43 5006
-10 47.54K 4 24.55K 24 10.41K 44 4827
-1 30.89K 5 23.46K 25 10.00K 45 4655
6 22.43K 26 9605 46 4480
7 21.45K 27 9227 47 4331
8 20.52K 28 8867 48 4179
9 19.63K 29 8523 49 4033
10 18.79K 30 8194 50 3893
11 17.89K 31 7880 51 3758
12 17.22K 32 7579 52 3629
60 2760 13 16.49K 33 7291 53 3504
70 1990 14 15.79K 34 7016 54 3385
80 1458 15 15.13K 35 6752 55 3270
90 1084 16 14.50K 36 6500 56 3160
100 816.8 17 13.90K 37 6258 57 3054
110 623.5 18 13.33K 38 6026 58 2952
120 481.8 19 12.79K 39 5805 59 2854
NOTE: Calibration parameter values are at line voltage of 120 VAC for 60Hz units or
220 VAC for 50Hz.
A. Install rotor and tie-down nut. Blacken one half of the top side of the tie-down nut
with a black ink marker.
B. Close door and set desired speed. Speed can be precisely set using ACCU-SET
button.
D. When the rotor reaches set speed, strobe the rotor through the strobe port in the
instrument door to verify the actual rotor speed with the display.
E. Display versus actual speed should be +/- 30 at 3750 RPM at nominal line voltage.
F. If there is a discrepancy adjust R16 on the display board. To increase value turn pot
clockwise, decrease value by turning counter-clockwise.
NOTE: Maximum speed must be reduced to 3250 RPM when using Micro Plus
Multiwell Carriers.
If set and indicated speed are not in agreement, the speed knob may need adjustment.
Process as follows:
1. Install rotor. Set the speed know to 3000 RPM and the timer to HOLD. When the
rotor speed stabilizes, adjust the speed knob until the display speed is 3000 RPM.
2. Loosen the set screws on the speed knob. Adjust the knob until it indicates 3000
RPM. Tighten the set screws.
Warning: To adjust drive current the screws around the front control panel have to
be removed and the panel leaned forward. This will prevent the latch from
keeping the door locked during drive operation. To operate the centrifuge
safely, a door tie-down strap must be used. The part number for
the door tie-down strap is 964455.
C. To operate the centrifuge safely during drive current calibration, use a tie-down strap
to hold door closed.
D. Engage the door latch mechanism by manually closing the jaws on the latch.
E. Connect DVM to TP1 (blk) and TP2 (yel). set DVM to DC volt scale (low range).
I. Verify during acceleration that the voltage representing drive current is .8VDC +
.05VDC. Adjust drive current pot R156 as the drive accelerates between 1000 -
2000 rpm. Voltage will decrease as drive reaches speed.
3.4 Troubleshooting
3.4.1 Resistance's*
120V Compressor
Common to Run 1.3 ohm
Common to Start 6.4 ohm
120V Condenser Fan 54 ohm
220V Compressor
Common to Run 5.9 ohm
Common to Start 26.5 ohm
220V Condenser Fan 235 ohm
Possible
Cause Result Recommended Action
Imbalance Display will - Turn timer OFF then turn power switch OFF.
detector indicate - Turning power switch back ON will clear diagnostic
tripped. "8880". error.
Rotor will - Open chamber door.
coast to - Be sure rotor is properly installed on the drive shaft.
stop, latch - Check to be sure the rotor buckets and adapters are
will not symmetrically loaded around the pivotal axis of the
open. bucket, with containers of equal weight and density
of
each other.
- Be sure the rubber boot is undamaged and is seated
correctly around the motor cover.
- Confirm instrument (Benchtop model) was installed
on a
sturdy laboratory table or counter.
Thermal " - Turn timer OFF then turn power switch OFF.
cutout - Turning power switch back ON will allow door to be
switch opened. Diagnostics will re-occur as long as motor
activated in is hot.
drive - (Non-refrigerated) Check air inlet filters in door for
motor. obstruction. Remove filters and clean.
- Be sure rotor is properly installed on drive shaft and
boot is positioned down around motor cover.
Tacho " - Turn timer OFF then turn power switch OFF.
meter - Turning power switch back ON will clear diagnostic
signal loss error.
- Check Tach signal at TP9. Signal should be
+12VDC
square wave. If you do to have signal, check Tach
pick-up
assembly located on bottom of drive assembly.
- Check Tach voltage at TP10.
- Check ACCU-SET, read set speed voltage at TP10.
- Check display board.
Rotor cannot *Line voltage Check line voltage while instrument is under
achieve set below rating. load.
speed. Replace brush set if worn less than 1/2 inch in
Commutator length.
worn. Replace drive motor assembly.
Motor failure
Replace drive motor assembly.
Door will not Power not on.
open. Rotor spinning Apply power to instrument.
over 40 RPM. Rotor must be spinning less than 40 RPM
Latch stuck. before door can be opened.
See Emergency Access, 3.4.4.
Rotor speed Tach voltage
shows "0000" loss. Check Tach Presence at DS3.
and rotor is Check Tach pulse at TP9, should be +12V.
spinning. Check Tach voltage at TP10, check
(ACCUSET) set speed voltage at TP10, check
Rotor speed display board.
display is Lost power to
blank. display. Check plug J14.
Fuse blow.
Rotor does Check F2 and power supply voltages.
not achieve Line voltage
set speed. below rating. Check Tach voltage while instrument is under
Speed load.
calibration is off.
Check speed verification, and speed knob
adjustment. Per sec 3.3
* Line voltage must be 120 VAC 60Hz or 220 VAC 50Hz to maintain top speed with
maximum load.
If the facility power fails only momentarily, the centrifuge will resume operation when
power is restored and the rotor will return to set speed. However, if the rotor comes to a
complete stop you will have to restart the run when the power is restored. In the event
of an extended power failure, it may be necessary to trip the door-locking mechanism
manually to remove the rotor and retrieve your sample.
1. Press the POWER switch to OFF and unplug the power cord from the power source.
4. Insert a 4-mm (5/32-inch) internal hex wrench into the access opening located on the
left side of the front panel.
5. Turn the hex wrench to the right (clockwise) until you hear a “click” or the door
opens.
3. The call:
A. Perform test run and check instrument operation. This includes display digits,
temperature control (R units), refrigeration system (R unit), motor operation.
B. DISCONNECT INSTRUMENT POWER by unplugging instrument power cord.
C. Remove chamber bowl and motor bellows (non-refrigerated) per step 3.1.4, front
panel per step 3.1.3.
D. Clean instrument. Clean or replace air filters.
E. Inspect and correct all instrument problems such as loose hardware, chaffing
burnt wires, pitted relay contacts, cracked hinges, cross-threaded rotor
F. Inspect rotors to insure they are maintained correctly per rotor instruction
manual.
G. Check instrument door operation to insure it functions correctly.
H. Perform all necessary instrument calibrations.
I. Reassemble instrument and make a final test run.
4. Call acceptance:
SECTION 4
SCHEMATICS
Power - When the power switch is turned on, the AC voltage goes through an auto
transformer, if required, (per wiring diagram for the country that it is being assembled
for) otherwise AC directly powers the input transformer which feeds the power supply
circuit, where +24V, +12V, +5V, and -6.2 DC are generated.
The refrigeration unit receives power through a sold state relay controlled by the low
voltage logic circuit. If the door is opened the refrigeration is turned off.
The door latch system has a solenoid and two switches built in. When the door is
closed with the rotor stopped, the door relay, controlled by low voltage circuits, allows
the front panel “DOOR” switch to energize the door solenoid. When the door latch is
opened, the other internal door latch switch is opened, disconnecting the power to the
high voltage circuit and the SCR bridge.
A power sense circuit detects lack of voltage on the high voltage side and asserts the
reset line on the low voltage circuit side. Once the door is closed the power to the high
voltage circuit is resumed and the reset line releases after one second, therefore the
system could be operated.
Start - The timer controls the start, stop, and time of the run. When the timer is turned
on or switched to the "HOLD" position, the door is locked, the tach confidence is
charged up and the drive relay is energized. When the timer is turned off brake current
is generated, and the rotor brakes or coasts to a stop. Below 40 RPM the door is
unlocked, therefore the latch solenoid can be energized, opening the door latch.
Tach, Speed Control, Error Detection - A crystal controlled oscillator circuit outputs a
fixed pulse width for the each tach pulse received. The fixed width tach pulses charge
up a tach confidence capacitor which maintains tach confidence. If you lose tach
pulses and
the timer is still on, a tach failure is generated after 20 seconds. This signal as well as
the imbalance, drive overtemp, or chamber overtemp (refrigerated models) will trigger
an error detection latch which disables the drive current command, and the drive will
coast to a stop. The door latch cannot be opened until the diagnostic error (8880) is
reset by a power off/on cycle. A frequency to voltage converter generates the tach
voltage which is shown on the display. The actual tach voltage is compared to the set
speed voltage. This signal controls the drive current command.
Brake - There are three possible settings for the brake switch on the front panel:
• Off - Removes braking entirely and allows the rotor to coast to a stop from set
speed.
• Low - Activates low dynamic braking down to 50 rpm, and is used for slower
deceleration to avoid re-mixing or re-suspension of sample.
• High - Activates high dynamic braking that starts immediately after timer is turned
off. When rotor speed reaches 50 rpm, all braking stops and the rotor coasts to a
stop.
During braking, the SCR's are fired but the resulting current flows through the motor
field winding only. The armature becomes a generator dissipating the power through
brake resistors, therefore braking.
Back EMF - If tach fails, there is a window in which the door relay could be energized
allowing the door latch to be opened. To eliminate this condition we monitor the back
EMF voltage from the armature.
The power supply transformer provides a 40 VAC on its secondary which is rectified by
CR8, 9, 10, and 11. The +24VDC (TP4) is filtered by C3 and supplies power to all
relays on the board. This voltage is used to generate the regulated +12V (TP6) and
+5V (TP7) using U8 and U9. The negative side of the bridge generates the -6.2 VDC
using R19 and VR1. It also generates a sync pulse that is taken to the high voltage
circuit, through opto transistor U5, this sync pulse is used in the constant current circuit
for the drive.
Reset
A momentary reset pulse is generated by U12 on power up, and anytime there is a
power interruption over 100 ms. The reset pulse; disables the SCR opto-coupler U6,
and the refrigeration s.s. relay. It discharges C24 and C37 to prevent false triggering of
the tach confidence, drive overtemp, chamber overtemp, and imbalance diagnostics.
Opening the chamber door generates a reset that is always high, turning off the
refrigeration s.s. relay.
Start
To start the instrument the timer has to be on and the chamber door closed. This takes
J14-9 to ground and U15F-6 low. U15F-11 is now high. U15G-10 goes low causing
U10G-10 to go high, charging up C38 through R132. AR6A-1 goes high after three
seconds causing U10A-16 go to low energizing the drive relay K3. When the timer was
turned on, U15E-5 went high and pin 12 low. Whenever the timer is either turned ON
or OFF, C40 will charge or discharge generating a 3 second pulse at the output of
AR6B-7. This pulse will charge up C41 and C32 via R8, CR36, and CR35, putting a
high on pin 13 of AR5D. AR5D-14 and AR4A-1 will go low (low = tach ok). Due to the
discharge rate of C41 and C32, there is enough time for the drive to start turning and
generate tach pulses to re-charge tach confidence capacitor C32. Therefore if there is
no diagnostic error turning on Q2 through CR17, Q2 will be off after the three second
pulse from AR6B-7.
Acceleration
When a run has been started, Q2 turns off and C25 starts charging up through R71, as
the voltage gradually increases, AR6D-14 output which was low starts oscillating.
Eventually its high level duty cycle increases to a solid high output which commands full
current to the drive. A full current causes rotor acceleration. The slope with which C25
gets charged up determines the soft start of the drive current.
Since the set speed is higher than actual speed, the output of AR2D-14 is high. The
high output doesn't effect the soft start operation due to CR15. At speed, AR2D-14
output regulates the voltage across C25 causing proper duty cycle on AR6D-14 to
regulate the current to the drive maintaining the set speed.
Tachometer Circuit
Tach pulses are generated by a magnetic sensor. There are six pulses per each rotor
revolution. The pulses can be viewed on DS3 or at TP9 using an oscilloscope. The
tach pulses trigger a one shot, AR5B, which enables a counter, generating a 572 micro
second pulse for each tach pulse received. Due to using a crystal controlled oscillator
and digital counter (U16), the pulse width is very stable and insensitive to the noisy
environment within the centrifuge. The fixed pulses charge up the tach confidence
capacitor C32 through R85. With C32 charged AR5D-14 stay low, and the system
continues to run. The same pulses charge up tach voltage capacitor C31 through R84.
The voltage across C31 indicates the exact speed of the rotor. Normally the tach
voltage is selected when U13A-10 is high, and displayed. Set speed voltage is selected
when U13A-10 is low, using Accu-set. Tach voltage can be observed at TP10. AR2A
and AR2B are used to buffer the actual speed and set speed voltages from multiplexer
U13A.
The temperature in the chamber is sensed by RT1, a 10K thermistor. The thermistor
output is linearized by resistors R124 and R125. AR3D is used to drive the temperature
meter, M1. The meter is a zero center type and is set up so that at 15°C the voltage is
exactly 1/2 of the bridge supply voltage, hence no zero adjust is required. R146 is used
to calibrate the temperature meter. Temperature setting is done by R1, a 10K variable
resistor. R90, R91, and R92 adjust the span of the set voltage to match the thermistor
output. When the output of AR3B-7 is low U15A-16 will go high turning the compressor
off via the solid state relay. AR3C provides over-temperature sensing. R95 and R96
set a voltage level corresponding to about 50°C. If the temperature exceed 50°C, the
output of AR3C-8 will go high causing AR4B-7 to go high after a time delay determined
by R9 and C24. When AR4B-7 goes high it causes AR6C-8 to go high. At this point
several conditions will follow:
1. This will turn on Q2 via R68, CR17, and R69. AR6D-14 will go low disabling opto-
transistor U2, current command to drive.
4. Q1 will turn on, allowing a high at J14-10. This will generate the diagnostic error
"8880" on the display. The diagnostic can be cleared by turning the power switch
off/on, after the temperature has dropped below 50°C.
The high voltage circuit is referenced to the line potential. An isolation box must be
used when probing this circuit. The high voltage circuit and the opto-SCR (U6) are
protected by F5 (1/4 amp) fuse. VR2 provides the +12 volt reference to AR1. This
circuit controls the firing angle required by the SCR bridge. The sync pulses from the
power supply circuit (U5) provide low going pulses at the output of comparator AR1B-7.
The low going pulses discharge the voltage across C14 through CR4. During the high
level of the pulses, C14 is charged up through R14; this generates a non-linear
sawtooth on the input of the comparator AR1A-3 which is used as a reference to
determine the firing angle of the SCR's. The non-linearity of the sawtooth is to
compensate for the non-linearity of the AC current so the resulting current adjustments
could be linear. U5 is designed in such a way that if it is open or missing from the
circuit, AR1A-3 would stay low and the SCR's would not fire. CR4 is selected to be a
IN4005 so that it could stand the high voltage potential across it in case of component
failures. R14 and R153 are selected 1 watt due to
a high voltage drop cross them not due to high power dissipation. AR1D-14 controls
the firing angle of the SCR bridge hence the current through the motor. Two loops
control this amplifier; the voltage across the current sense resistor, R3, through R5 a
33.2K resistor, and the pulse train, generated by AR6D-14, sent to the high voltage
circuit through opto-transistor U2. Pulses are converted to a voltage level by R25 and
C6.
The currents at the summing node AR1D-13 generate a voltage level at AR1D-14 which
gets compared to the non-linear sawtooth by comparator AR1A, resulting in a high
going pulse at AR1A-1. This high going pulse turns on the opto-transistor U3 which
forces a low going pulse at U11G-10 turning on DS1, the current command LED and
U6, the opto-SCR. This pulse can be observed at TP-8 using an oscilloscope. The
opto-SCR is controlled at different firing angles based on the pulse widths of the trigger
pulse, thereby firing the gates of the SCR bridge for different duration’s. The voltage
that is generated on the + side the of SCR bridge is filtered through a choke to reduce
the RMS line current. When in brake mode the drive relay K3 is de-energized, allowing
current to flow through the motor field winding only. The voltage generated by the
armature is dissipated through the 100 watt brake resistor and at 1000 RPM a 25 watt
brake resistor is paralleled by K4A relay contracts.
If a tach failure happens, the drive starts accelerating for 6 seconds and then goes into
a coast mode. The prevent the operator from activating the door latch, the circuit
disables the door relay K5 by monitoring the back EMF generated by the armature.
This voltage is sensed by AR1C-9, turning the opto-transistor U4 on. This will put a
high U10D-4 causing a low at U10D-13 preventing the door solenoid from energizing.
Back EMF is generated down to 250 RPM, at this time U4 is turned off causing a low at
U10D-4. With U10D-13 open, R5D-14 (tach presents) will control the door latch relay
K5. In normal operation, when the RPM is less than 40, tach confidence discharges
and AR5D-14 goes high, activating the door latch relay K5. This allows the operator to
open the door using the door switch.
Stop
To terminate a run the timer is turned off. U15F-6 will go high and U15F-11 low.
U15G-10 will go high causing U10G-10 to go low discharging C38. Therefore releasing
the drive relay K3 when AR6A-1 goes low after one second. Meanwhile the rising edge
on U15E-12 triggers the one shot AR6B, generating a three second high going pulse
which turns Q2 on . When Q2 is turned on capacitor C25 is discharged, the oscillator
output of AR6D-14 goes low turning of opto-transistor U2 stopping the current to the
motor windings due to K3 being de-energized. The generated armature voltage is
dissipated through the 100W brake resistor. Max. brake relay K4 is energized below
1000 rpm. If the brake is off the rotor will coast to a stop.
Brake Circuit
Braking is performed by either low dynamic braking or high dynamic braking. During
brake mode, K3 is de-energized and SCR voltage is applied to the field windings of the
motor. This results in a generated EMF in the armature which causes current to flow
through the 13 ohm resistor, giving low dynamic braking. At low speeds, < 1000 RPM,
the EMF is lower, which requires an additional resistor in the circuit to maintain braking
down to 50 RPM. When high brake is selected, K6 is energized for additional dynamic
braking. If no brake is selected, the motor field is not energized.