Instructions For Use

Allegra X-5

Centrifuge
For In Vitro Diagnostic Use

PN B29071AA
July 2013

Beckman Coulter, Inc.

250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.
Allegra X-5
Centrifuge
PN B29071AA (July 2013)

Copyright ©2013 Beckman Coulter, Inc.

All rights reserved

No part of this document may be reproduced

or transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or
otherwise, without prior written permission from
Beckman Coulter, Inc.

Beckman Coulter, the stylized logo, and Allegra are

trademarks of Beckman Coulter, Inc. and are registered in
the USPTO.

All other trademarks, service marks, products, or services

are trademarks or registered trademarks of their
respective holders.

Find us on the World Wide Web at:

www.beckmancoulter.com

Beckman Coulter Ireland, Inc.

Mervue Business Park, Mervue Galway, Ireland 353 91 774068

Made in Germany
Printed in the U.S.A.
 Revision Status
Initial Issue, 07/2013
Allegra X-5 Centrifuge Instructions For Use version B29071AA

PN B29071AA iii
 Revision Status

iv PN B29071AA
 Safety Notice
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to operate the instrument. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as
to how to proceed in any situation, contact your Beckman Coulter Representative.

Alerts for Danger, Warning, Caution, Important, and Note

DANGER
DANGER indicates an imminently hazardous situation which, if not avoided,
will result in death or serious injury.

WARNING
WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.

CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury. It may also be used to alert against unsafe
practices.

IMPORTANT IMPORTANT is used for comments that add value to the step or procedure being performed.
Following the advice in the Important adds benefit to the performance of a piece of equipment or to a
process.

NOTE NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.

PN B29071AA v
 Safety Notice
Safety During Installation and/or Maintenance

Safety During Installation and/or Maintenance

WARNING
Risk of personal injury or equipment damage. The centrifuge weighs 77 kg/169 lb.
DO NOT attempt to lift or move it without assistance from a lifting device and/or
another person.

WARNING
Risk of personal injury. Any servicing of this equipment that requires removal of
any covers can expose parts which involve the risk of electric shock or personal
injury. Make sure that the power switch is turned off and the instrument is
disconnected from the main power source by removing the Main (power) plug
from the outlet receptacle, and refer such servicing to qualified personnel.

Do not replace any centrifuge components with parts not specified for use on this instrument.

Electrical Safety

To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to
connect this equipment to earth-ground. To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.
Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to
the instrument.
• Never use a three-to-two wire plug adapter.
• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet
receptacle strip.
• Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get
into the instrument and damage electrical or mechanical components.
• Work on the power supply system must be performed by certified electricians.
• Inspect the electrical equipment of the unit regularly. Defects such as loose or burnt cables
must be eliminated immediately.

vi PN B29071AA
 Safety Notice
Safety Against Risk of Fire

Safety Against Risk of Fire

WARNING
Risk of personal injury or equipment damage. This centrifuge is not designed for
use with materials capable of developing flammable or explosive vapors. Do not
centrifuge such materials (such as chloroform or ethyl alcohol) or materials which
could react chemically with sufficient vigor to cause a HAZARD, nor handle or store
them within the 30 cm (1ft) clearance envelope surrounding the centrifuge. Do not
use the centrifuge in hazardous locations.

Mechanical Safety

For safe operation of the equipment, observe the following:

• Use only the rotor and accessories designed for use in this centrifuge.
• Before starting the centrifuge, make sure that the rotor tie-down screw is securely fastened.
• Do not exceed the maximum rated speed of the rotor in use.
• NEVER attempt to slow or stop the rotor by hand.
• Do not lift or move the centrifuge while the rotor is spinning.
• If a glass tube breaks inside the chamber bowl, be careful when examining or cleaning the
gasket or chamber, as sharp glass fragments may be embedded in their surfaces.
• NEVER attempt to override the door interlock system while the rotor is spinning.
• Maintain a 30 cm (12 in.) clearance envelope around the centrifuge while it is running. During
operation you should come within the envelope only to adjust instrument controls, if
necessary. Never bring any flammable substances within the 30 cm (12 in.) area surrounding
the centrifuge. Never lean on the centrifuge or place items on the centrifuge while it is
operating.
• Never operate the instrument without a rotor installed.

Chemical and Biological Safety

Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or
radioactive. Such materials should not be used in this instrument unless all necessary safety
precautions are taken.

• Observe all cautionary information printed on the original solution containers prior to
their use.
• Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that they are free of micro-organisms. Some of the most virulent— Hepatitis (B and
C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi—further emphasize
the need for aerosol protection. Handle other infectious samples according to good laboratory

PN B29071AA vii
 Safety Notice
Chemical and Biological Safety

procedures and methods to prevent spread of disease. Because spills may generate aerosols,
observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or
radioactive materials in this centrifuge without taking appropriate safety precautions. Biosafe
containment should be used when Risk Group II materials (as identified in the World Health
Organization Laboratory Biosafety Manual) are handled; materials of a higher group require
more than one level of protection.
• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.
• Do not centrifuge materials that could result in a hazardous chemical reaction.

It is your responsibility to decontaminate the centrifuge and accessories before requesting service
by Beckman Coulter.

viii PN B29071AA
 Contents

Revision Status, iii

Safety Notice, v
Alerts for Danger, Warning, Caution, Important, and Note, v
Safety During Installation and/or Maintenance, vi
Electrical Safety, vi
Safety Against Risk of Fire, vii
Mechanical Safety, vii
Chemical and Biological Safety, vii

Introduction, xv
Intended Use, xv
Certification, xv
Scope of Manual, xv
Conventions, xvi
Typographic Conventions, xvi
CFC-Free Centrifugation, xvi
Symbols and Labels, xvi
Biohazard, xvii
Caution Symbol, xvii
High Voltage Danger, xvii
Protective Ground, xvii
Recycling Label, xviii
Risk of Fire Warning, xviii
Electric Shock Warning, xviii
Rotor Rotation, xix
China RoHS Caution Label, xix
Australian EMC Compliance Label, xx
CE Label, xx
Consult IFU Label, xx
IVD Label, xx
RoHS Conform Label, xxi

ix
 Contents

TUV-NRTL Label, xxi

CHAPTER 1: Description, 1-1

Introduction, 1-1
Centrifuge Function and Safety Features, 1-1
Centrifuge Function, 1-2
Safety Features, 1-2
Chassis, 1-3
Housing, 1-3
Door, 1-3
Rotor Chamber, 1-3
Drive, 1-4
Controls and Indicators, 1-4
Power Switch, 1-4
Control Panel, 1-5
System Keys, 1-5
Program Keys, 1-6
Digital Displays, 1-7
Name Rating Plate, 1-8
Specifications, 1-8
Rotor, 1-10

CHAPTER 2: Operation, 2-1

Introduction, 2-1
Run Procedure, 2-2
Preparation and Loading, 2-2
Entering Run Parameters, 2-4
Setting Run Speed, 2-5
Setting RPM, 2-5
Setting RCF, 2-5
Setting Run Time, 2-6
Setting Acceleration Rate, 2-6
Setting Deceleration Rate, 2-7
Starting a Run, 2-7
Pulse Function, 2-8
Changing Parameters During a Run, 2-9
Stopping a Run, 2-9
Unloading, 2-10
Enabling or Disabling the Audible Tones, 2-10
Summary of Run Procedures, 2-11

CHAPTER 3: Troubleshooting, 3-1

Introduction, 3-1

x
 Contents

User Messages, 3-1

Other Possible Problems, 3-3
Emergency Door Release, 3-3

CHAPTER 4: Care and Maintenance, 4-1

Introduction, 4-1
Maintenance, 4-1
Preventive Maintenance, 4-1
Cleaning, 4-2
Tube Breakage, 4-3
Decontamination, 4-3
Sterilization and Disinfection, 4-3
Storage and Transport, 4-4
Storage, 4-4
Notes on Transport, 4-4
Transport Safety Device, 4-4
Returning a Centrifuge, 4-5
Supply List, 4-6
Replacement Parts, 4-6

APPENDIX A: Installation, A-1

Introduction, A-1
Installing the Instrument, A-1
Transportation Safety Devices, A-2
Transport Safety Device, A-2
Electrical Requirements, A-3
Test Run, A-5

APPENDIX B: Table of Hazardous Substance’s

Name and Concentration, B-1
, B-1

Beckman Coulter, Inc.

Allegra X-5 Centrifuge Warranty

xi
 Illustrations

Illustrations

1.1 Front View, 1-1

1.2 Back View, 1-2
1.3 Interior View of the Rotor Chamber, 1-4
1.4 The Power Switch, 1-5
1.5 The Control Panel, 1-5
1.6 Digital Displays, 1-7
2.1 Tapered Sleeve Position, 2-3
2.2 Default Parameters, 2-5
3.1 Location of the emergency door release. opening., 3-4
3.2 Insertion of Allen wrench., 3-5
4.1 Locking Screws (Transport Safety Device), 4-5
A.1 Locking Screws (Transport Safety Device), A-3
A.2 Dimensions of the Centrifuge, A-4

xii PN B29071AA DRAFT

 Tables

Tables

2.1 Acceleration/Deceleration Times (in minutes:seconds), 2-7

3.1 Error Message Chart, 3-2
3.2 Troubleshooting Chart, 3-3

PN B29071AA DRAFT xiii

 Tables

xiv PN B29071AA DRAFT

 Introduction

Intended Use

For In Vitro Diagnostic Use.

This Allegra X-5 centrifuge is intended for the separation of components through the use of relative
centrifugal force.

It is designed to separate human samples, including blood and other body fluids, for processing,
analysis, and in vitro diagnostic testing, as well as non-human body samples and chemicals,
including industrial and environmental samples.

This centrifuge should be operated by qualified personnel only.

Certification

The Beckman Coulter Allegra X-5 Centrifuge is manufactured in a facility that maintains
certifications to ISO 9001:2008 or ISO 13485:2003. It has been designed and tested to be compliant
(when used with Beckman Coulter rotors) with the laboratory equipment requirements of
applicable regulatory agencies. Declarations of conformity and certificates of compliance are
available at www.beckmancoulter.com.

Scope of Manual

This manual is designed to familiarize you with the Beckman Coulter Allegra X-5 Centrifuge, its
functions, specifications, operation, and routine operator care and maintenance. Beckman Coulter
recommends that you read this entire manual, especially the Safety Notice section and all safety
related information, before operating the centrifuge or performing instrument maintenance.

The following introductory pages contain the instrument specifications, as well as space,
electrical, and temperature conditions required for optimal centrifuge performance. A list of
available rotors is also included.
• CHAPTER 1, Description provides a brief physical and functional description of the centrifuge
and the operating controls and indicators.
• CHAPTER 2, Operation contains centrifuge operating procedures.

PN B29071AA xv
 Introduction
Conventions

• CHAPTER 3, Troubleshooting lists diagnostic messages and other possible malfunctions,

together with probable causes and suggested corrective actions.
• CHAPTER 4, Care and Maintenance contains procedures for routine operator care and
maintenance, as well as a brief list of supplies and replacement parts.
• APPENDIX A, Installation contains instructions for installing and connecting the centrifuge.

NOTE If the centrifuge is used in a manner other than specified in this manual, the safety and performance
of this equipment could be impaired. Further, the use of any equipment other than that recommended
by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically
recommended in this manual and/or the applicable rotor manual is the sole responsibility of the user.

Conventions

Certain symbols are used in the product labeling to call out safety-related and other important
information. These international symbols may also be displayed on the centrifuge and are
reproduced on the inside of the back cover of this manual.

Typographic Conventions
Certain typographic conventions are used throughout this manual to distinguish names of user
interface components, such as keys and displays.

• Key names (for example, START or ENTER) and display names (for example, TIME or SPEED)
appear in bold type.
• Cursor keys, used to increment values up or down when setting parameters, are shown as up and
down arrows (s or t).

CFC-Free Centrifugation

To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of
Allegra X-5 centrifuges.

Symbols and Labels

This section provides information for some labels and symbols appearing on the Allegra X-5
instrument housing. These labels and symbols may be associated with user-serviceable procedures.
Individual hazards associated with a specific procedure in this manual may use these labels and
symbols, and are included in Warnings or Cautions within the procedures for that task.

xvi PN B29071AA
 Introduction
Symbols and Labels

Biohazard

This caution symbol indicates biohazardous risk from possible patient specimen contamination.

Caution Symbol

This symbol indicates a caution message and appears adjacent to an explanation or other symbols
that define the caution.

High Voltage Danger

This symbol indicates high voltage is present or that there is a risk of electric shock when working
in this area. Operation, replacement or servicing of any components where contact with bare, live
hazardous parts could occur, possibly resulting in electric shock, should only be performed by your
Beckman Coulter representative.

Protective Ground

This symbol is used to indicate a protective ground. This instrument must be properly grounded.
Do not under any circumstances operate the instrument unless it is properly grounded.

PN B29071AA xvii
 Introduction
Symbols and Labels

Recycling Label

A28219-AA

This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of this marking on the product indicates:

• the device was put on the European Market after August 13, 2005 and
• the device is not to be disposed of via the municipal waste collection system of any member
state of the European Union.

It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing
this label, please contact your dealer or local Beckman Coulter office for details on the take-back
program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal
of the device.

Risk of Fire Warning

Before replacing fuses, shut off power and disconnect the power cord. Failure to do so can cause
electric shock and/or equipment damage. Replace fuses only with approved type and rating
replacement fuse.

Electric Shock Warning

Operation, replacement or servicing of any components where contact with electronic

components could occur can result in electric shock, and should only be performed by your
Beckman Coulter representative.

xviii PN B29071AA
 Introduction
Symbols and Labels

Rotor Rotation

This label indicates the direction of the rotor rotation.

China RoHS Caution Label

This label and materials declaration table (APPENDIX B, Table of Hazardous Substance’s Name and
Concentration) are to meet People's Republic of China Electronic Industry Standard SJ/T11364-2006
“Marking for Control of Pollution Caused by Electronic Information Products” requirements.

This label indicates that this electronic information product contains certain toxic or hazardous
elements, and can be used safely during its environmental protection use period. The number in the
middle of the logo is the environmental protection use period for the product.The outer circle
indicates that the product can be recycled. The logo also signifies that the product should be
recycled immediately after its environmental protection use period has expired. The date on the
label indicates the date of manufacture.

PN B29071AA xix
 Introduction
Symbols and Labels

Australian EMC Compliance Label

The C-Tick mark is intended for use on products that comply with Australian Communication
Authority (ACA) EMC Requirements.

CE Label

This label indicates conformance to various directives set forth under European Union law.
The Declaration of Conformity available at www.beckman.com indicates the specific directives or
standards.

Consult IFU Label

This label indicates that the Instructions for Use should be referred to for more information.

IVD Label

This label indicates an In Vitro diagnostic medical device.

xx PN B29071AA
 Introduction
Symbols and Labels

RoHS Conform Label

This label indicates that the product is compliant with the EU RoHS directive (2002/95/EC)
pertaining to the Restriction of Hazardous Substances in Electrical and Electronic Equipment (EEE).

TUV-NRTL Label

This label indicates recognition by a Nationally Recognized Test Laboratory (NRTL) that the
instrument has met the relevant product safety standards.

PN B29071AA xxi
 Introduction
Symbols and Labels

xxii PN B29071AA
 CHAPTER 1

Description

Introduction

This chapter provides a brief physical and functional description of the Beckman Coulter Allegra X-5
Centrifuge. The operating controls and indicators are also described; instructions for their use are in
CHAPTER 2, Operation. Chemical compatibilities of materials listed in this manual can be found in
Chemical Resistances (publication IN-175). Refer to the applicable rotor manuals for rotor descriptions.

Centrifuge Function and Safety Features

Figure 1.1 Front View

1. User Interface
2. Door
3. Main Power Switch

PN B29071AA 1-1
 Description
Centrifuge Function and Safety Features

Figure 1.2 Back View

1. Main Power Switch

2. Ground Screw
3. Mains Power Input

Centrifuge Function
The Beckman Coulter Allegra X-5 bench top centrifuge generates centrifugal forces required for a
variety of applications. Together with the Beckman Coulter rotor designed specifically for use in
this centrifuge, the applications include:

• Routine clinical sample processing of serum, plasma, whole blood and other body fluids.
• Rapid sedimentation of protein precipitates, particulates and cells from clinical samples.
• Processing of samples for in-vitro cytotoxicity studies, receptor binding studies and other
specialized tests.

The centrifuges are microprocessor-controlled, providing interactive operation. The instrument

design features a brushless three-phase drive system, automatic rotor overspeed identification
system, and a choice of acceleration/deceleration rates. User messages and a series of audible tones
alert the operator to conditions that may need attention. (Instructions for disabling the audible
tones are in CHAPTER 2, Operation.)

Safety Features
The Allegra X-5 Centrifuge has been designed and tested to operate safely indoors at altitudes up to
2000 m (6562 ft).

Instrument safety features include:

• An electromechanical door-locking mechanism prevents operator contact with spinning

rotors. When the door is closed it locks automatically. It can be unlocked only by pressing the
OPEN DOOR key, and opened only when the power is on and the rotor is at rest.
• A steel barrier surrounds the rotor chamber to provide full operator protection.

1-2 PN B29071AA
 Description
Chassis 1

• An overspeed system continuously monitors the rotor during centrifugation. The system
includes a magnetic sensor on the drive motor. Throughout the run, checks are made to ensure
that the rotor does not exceed set speed.
• An imbalance detector monitors the rotor during the run, causing automatic shutdown if rotor
loads are severely out of balance. If the Audible Tones are enabled, a sound signal (beep) is also
emitted (see CHAPTER 2, Enabling or Disabling the Audible Tones). At low speeds, an incorrectly
loaded rotor can cause imbalance. Rotor instability can also occur if the centrifuge is moved
while running, or if it is not resting on a level surface.
• The centrifuge feet, made of rubber, have been designed to minimize possible rotation in the
event of a rotor mishap.

Chassis

Housing
The centrifuge housing is made of sheet steel, finished with urethane paint. The control panel is
covered by a protective overlay made of coated polycarbonate (Figure 1.1 and Figure 1.2).

Door
The door is made of a solid sheet of steel, encased in foam molding. In the center of the door is a
window for strobe viewing. The door is secured to the housing by two hinges. An electromechanical
door lock system prevents operator contact with spinning rotors and prevents run initiation unless
the door is shut and latched. The door is locked when a run is in progress and can be opened only
when the rotor is stopped. (A light-emitting diode [LED] on the OPEN DOOR key lights up when the
door can be opened.) In the event of a power failure, the door lock can be manually released for
sample recovery (see CHAPTER 3, Troubleshooting).

Rotor Chamber
The rotor chamber is shown in Figure 1.3. The drive shaft, mounting plate, and a rubber boot
surrounding the drive shaft are visible in the chamber bottom. A gasket system around the chamber
opening ensures sealing. (Instrument gaskets have not been designed as bioseals for aerosol
containment.)

PN B29071AA 1-3
 Description
Controls and Indicators

Figure 1.3 Interior View of the Rotor Chamber

1. Drive Shaft 3. Boot

2. Tapered Sleeve 4. Gasket

Drive
The asynchronous three-phase direct-drive motor is brushless for clean, quiet operation. A tie-
down screw is used to attach the rotor to the drive shaft. The resilient suspension ensures that loads
are not disturbed by vibration, and prevents damage to the drive shaft if an imbalance occurs
during centrifugation. Maximum braking may be selected to reduce deceleration time, allowing fast
processing of samples; alternately, delicate gradients may be preserved using slower deceleration.

Controls and Indicators

Power Switch
The power switch is located on the centrifuge right side panel (see Figure 1.4). This two-position
rocker switch (I, on; O, off) controls electrical power to the centrifuge.

1-4 PN B29071AA
 Description
Controls and Indicators 1

Figure 1.4 The Power Switch

1. Power Switch

NOTE The power must be turned on before the chamber door can be opened.

Control Panel
The control panel is mounted at an angle on the front of the centrifuge and includes system keys,
programming keys, and digital displays (see Figure 1.5). The panel also contains an IMBALANCE light
that flashes if rotor loads are severely out of balance.

Figure 1.5 The Control Panel

System Keys
The centrifuge operation is controlled through the system keys. Each key (except the PULSE key)
has an LED in the upper left corner that lights to indicate that the key can be activated.

PN B29071AA 1-5
 Description
Controls and Indicators

START
Pressing the START key causes the centrifuge run to begin. This key can also be used
to abort a deceleration process and restart the centrifuge.

STOP The STOP key can be pressed to end a run. It operates in two modes, depending on how
you press it:
• Normal stop (press and release):
The centrifuge decelerates to a complete stop according to the preselected
deceleration rate. Deceleration can be terminated and the centrifuge restarted by
pressing START again. The centrifuge emits a series of audible tones when the rotor
reaches 0 RPM. (Instructions for disabling the tones are in CHAPTER 2, Operation.)
• Fast stop (press and hold for at least two seconds):
The centrifuge decelerates to a complete stop at the maximum rate. The
deceleration cannot be interrupted; the centrifuge can only be restarted after the
rotor stops and the door is opened and closed.
OPEN DOOR Pressing the OPEN DOOR key unlatches the centrifuge door locks and allows the door
to be opened. The centrifuge accepts this command only when the rotor is completely
stopped and the OPEN DOOR key LED is lit.
PULSE Pressing the PULSE key causes the installed rotor to accelerate at the maximum rate
up to the set speed for short-duration runs (as long as the key is pressed). Deceleration,
at the maximum rate, begins when the key is released.

Program Keys
The program keys are used to set run parameters (a program consists of all of the parameters for a
run). Except for the cursor and ENTER keys, program keys are located beneath the applicable digital
displays, which show the parameters as they are input. Each key (except for the cursor keys) has an
LED in the upper left corner that lights to indicate operational readiness. The LEDs also blink if an
incorrect parameter is entered.

Cursor Keys The cursor keys are up and down arrow keys, which can be pressed to increment values
up or down when setting parameters.

ENTER
Parameter (speed, time, temperature, and acceleration or deceleration profile)
changes made while a run is in progress must be verified by pressing the ENTER key.

RPM When the RPM key is pressed the last digit in the RPM/RCF/ROTOR display (0) flashes,
indicating that the speed can be entered in increments of 100 revolutions per minute
(RPM). After the run starts, the actual RPM of the rotor is displayed.

RCF The RCF key can be used to select the speed setting by required relative centrifugal
field (RCF). The corresponding RPM is automatically calculated and displayed during
the run. If the RCF key is pressed during the run, the RCF value is shown in the RPM/
RCF/ROTOR display.
ROTOR
Pressing the Rotor key displays the rotor number.

1-6 PN B29071AA
 Description
Controls and Indicators 1

TIME The TIME key is used to select the run duration. When the TIME key is pressed, the last
digit on the TIME display flashes, indicating that the time can be entered with the
cursor keys.
• Timed run — Run time up to 9 hours and 59 minutes can be set. If the minutes
parameter exceeds 59, it is automatically converted into hours.
• Continuous run — If a run time of less than 1 minute or more than 9 hours and
59 minutes is selected, continuous operation is activated, and an infinity symbol
(∞) is displayed. Time is not counted down, and the run continues until the STOP
key is pressed.
ACCEL The ACCEL key is used to select acceleration rates that protect delicate gradients.
When the ACCEL key is pressed, the ACCEL/DECEL display flashes, indicating that one
of ten preset rates can be entered with the cursor keys (9 is the fastest rate and 0 is
the slowest rate). Acceleration rates are described in CHAPTER 2, Acceleration/
Deceleration Times (in minutes:seconds).
DECEL The DECEL key is used to select deceleration rates that maintain optimum separation
while protecting delicate gradients. When the DECEL key is pressed, the ACCEL/DECEL
display flashes, indicating that one of ten preset rates can be entered with the cursor
keys (9 is the fastest rate and 0 is a no-brake coast to stop). Deceleration rate
selections are described in CHAPTER 2, Acceleration/Deceleration Times (in
minutes:seconds).

Digital Displays
Digital displays indicate rotor speed, run time, and numbers that represent selected acceleration
and deceleration profiles (see Figure 1.6). When the power is turned on, they show the most
recently entered parameters, before the power was turned off. The displays serve a dual purpose:

• When the run parameters are being set (the input mode), the displays show the set values
(those selected by the operator). When a run parameter key (for example, TIME or RPM) is
pressed, the appropriate display flashes to indicate that data can be entered.
• The actual (real-time) operating conditions of the centrifuge are displayed during the run, after
START is pressed.

NOTE Error messages (see Table 3.1, Error Message Chart) also appear on the displays, when applicable. The
centrifuge emits a series of audible tones to alert the user to an error condition.

Figure 1.6 Digital Displays

PN B29071AA 1-7
 Description
Name Rating Plate

RPM/RCF/ROTOR • In input mode the RPM/RCF/ROTOR display shows the value of the parameter
being set, depending on the programming key pressed (RPM, RCF, or ROTOR).
For example, if the ROTOR programming key is pressed,the rotor number
appears on the RPM/RCF/ROTOR display.
• During centrifugation, the RPM/RCF/ROTOR display shows the speed of the rotor
in RPM. If the RCF key is pressed while the centrifuge is running, the RCF value
is displayed.
TIME • During a timed run (between 1 minute and 9 hours, 59 minutes), the TIME
display begins counting down when the rotor starts to spin and continues the
countdown until deceleration begins. The TIME display indicates the remaining
run time in hours and minutes.
• During a continuous run (less than 1 minute or more than 9 hours, 59 minutes
selected), countdown time is not displayed. Instead, the infinity (¥) symbol,
indicating continuous operation, lights up and the TIME display shows time
elapsed since the run start. After 9 hours and 59 minutes the timer resets to 0
and continues counting elapsed time.
ACCEL/DECEL The ACCEL/DECEL display shows the acceleration profile that was selected for the
run. The deceleration profile number can be displayed by pressing the DECEL key.

Name Rating Plate

The name rating plate is affixed to the right side of the centrifuge. Check that the line voltage agrees
with the voltage listed on this name rating plate before connecting the centrifuge. Always mention
the serial number and the model number shown when corresponding with Beckman Coulter
regarding your centrifuge.

Specifications

Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data,
without guarantee.

Speed
Set speed 100 to 4700 RPM (in 100-RPM increments)
Speed control actual rotor speed, ±50 RPM of set speed
Speed display actual rotor speed in 100-RPM increments or in RCF (when selected)
Time
Set time 1 min. to 9 hr 59 min, (in 1 min. increments),
Time display time remaining in run (timed run ±1 minute) or
¥ and elapsed time (continuous run)
Acceleration 10 acceleration rates
Deceleration 10 deceleration rates
Ambient temperature range 5 to 40°C
Humidity restrictions <80% (noncondensing)

1-8 PN B29071AA
 Description
Specifications 1

Maximum altitude 2000 m (6562 ft).

Dimensions
Width 49.6 cm (19.5 in.)
Depth 63.4 cm (25.0 in.)
Height, door closed 39.0 cm (15.3 in.)
Height, door open 88.0 cm (34.6 in.)
Weight 77 kg (169 lb)
Clearances (sides) 30 cm (11.8 in.)
Electrical requirements
120-V instrument 120 VAC, 12A, 60 Hz
200 V instrument 200 VAC, 10A, 50/60 Hz
220–240-V instrument 220–240 VAC, 9A, 50/60 Hz
Electrical supply Class I
Maximum heat dissipation into room 3750 BTU/h (1.1 kW)
under steady-state conditions
Noise level at max. speed (approx.) ≤67 dBa
Installation (overvoltage) category II
Pollution degreea 2
a. Normally only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation must be expected.

PN B29071AA 1-9
 Description
Rotor

Rotor

See the SX4700 User Manual for information on rotor use, care and maintenance, and rotor
accessories.

Rotor Profile Description Max RPM Max RCF (x g) Max Capacity Rotor Part
(mL) Number/
Rotor Manual
Number
SX4700 Swinging 4700 4470 100 x 10 mL B30593/
Bucket 140 x 5/7 mL B30343
rmax = 181 mm

1-10 PN B29071AA
 CHAPTER 2

Operation

Introduction

This section contains operating procedures for the centrifuge, using the Beckman Coulter rotor designed
for use in this centrifuge. Refer to the rotor manual for instructions on preparing the rotor for
centrifugation.

NOTE If the centrifuge is used in a manner other than that specified in this manual, the safety and
performance of this equipment could be impaired.

WARNING
Risk of personal injury or contamination. Normal operation may involve the use of
solutions and test samples that are pathogenic, toxic, or radioactive. Operator
error or tube failure may generate aerosols. Do not run potentially hazardous
materials in this centrifuge unless all appropriate safety precautions are taken.
Always use the appropriate rotors and adapters.

Handle all infectious samples according to good laboratory practices and methods
to prevent the spread of disease. Ask your laboratory safety officer to advise you
about the level of containment required for your application and about the proper
decontamination or sterilization procedures to follow if fluids escape from
containers. Biosafe containment should be used when Risk Group II materials (as
identified in the World Health Organization Laboratory Biosafety Manual) are
handled; materials of a higher group require more than one level of protection.
Because spills may generate aerosols, observe proper safety precautions
for aerosol containment.

PN B29071AA 2-1
 Operation
Run Procedure

WARNING
Risk of personal injury or property damage. The centrifuge must not be used in the
vicinity of flammable liquids or vapors, and such materials should not be run in the
centrifuge. During operation you should come within the 30 cm (12 in.) clearance
envelope only to adjust the instrument controls, if necessary. Never bring any
flammable substances within the 30 cm (12 in.) area surrounding the centrifuge.
Do not lean on the centrifuge or place items on the centrifuge while it is operating.

Run Procedure

The following detailed operating procedures are summarized at the end of this section. If you
are an experienced user of this centrifuge, you can turn to the summary for a quick review of
operating steps.

Preparation and Loading

NOTE Before installing the rotor, lubricate it following the instructions in the rotor manual.

1 Check the name rating plate for the correct voltage, then plug the power cord into the wall
receptacle.

2 Press the power switch to on (I).

3 Press the OPEN DOOR key and lift the door up; it remains in the open position.

4 Use the T-handle wrench to turn the rotor tie-down screw to the left (counterclockwise).
a. Remove the tie-down screw.

5 Make sure that the tapered sleeve is in place at the base of the centrifuge drive shaft before
installing the rotor (see Figure 2.1) and wipe the sleeve to be sure that it is clean and dry.
• The rotor rests on the sleeve while spinning, and does not operate properly if the sleeve is
missing.

2-2 PN B29071AA
 Operation
Run Procedure 2

Figure 2.1 Tapered Sleeve Position

1. Tapered Sleeve

NOTE If the tapered sleeve comes off, it must be replaced by a Beckman Coulter Field Service
representative. Call Beckman Coulter Field Service (1-800-742-2345 in the United States; outside the
U.S. contact your local Beckman Coulter office or visit us at www.beckmancoulter.com).

CAUTION
Risk of equipment damage. Do not drop the rotor onto the drive shaft. The shaft
can be damaged if the rotor is forced sideways or dropped onto it. Install the rotor
by centering it over the shaft and carefully lowering it straight down.

6 Install the rotor according to the instructions in the rotor manual.

a. Lower the rotor straight down onto the drive shaft.

b. Tighten the tie-down screw clockwise onto the drive shaft.

PN B29071AA 2-3
 Operation
Run Procedure

c. Seat the filled buckets onto the yoke pins.

• Be sure to fill all positions on the yoke with buckets.

CAUTION
Risk of equipment damage. An improperly loaded rotor can cause severe
vibration. Always run the rotor with a balanced load.

7 Close the centrifuge door and push firmly down on both sides of the door front until you hear
a clicking (latching) sound.

8 Remove the rotor from the centrifuge if a long period between runs is anticipated.

CAUTION
If the rotor is left in the centrifuge between runs, make sure the rotor is seated on
the drive shaft and the tie-down screw is tight before each run.

Entering Run Parameters

When the power is applied for the first time (no previous runs), default values are displayed (see
Figure 2.2). After the initial use, the parameters of the most recent run are displayed when power
is applied.

2-4 PN B29071AA
 Operation
Run Procedure 2

Figure 2.2 Default Parameters

1. Accelerate rotor to 2000 RPM.

2. Continue run for two minutes.
3. Use acceleration profile 8.

Setting Run Speed

Centrifuge speed can be set for up to the maximum rated speed of the rotor. Either revolutions per
minute (RPM) or relative centrifugal field (RCF) can be used to select speed. During centrifugation,
the RPM/RCF/ROTOR display indicates the actual run speed (RPM) of the rotor.

Setting RPM

1 Press the RPM key.

• The last digit on the RPM/RCF/ROTOR display (0) flashes, indicating that the RPM can be
entered (in 100-RPM increments) with the cursor keys.

2 Press the s or t cursor key until the required RPM is displayed.

• The corresponding RCF is automatically calculated by the centrifuge, but the RPM value is
displayed during the run.
• You can check the RCF during the run by pressing the RCF key while the centrifuge is
running.

Setting RCF

1 Press the RCF key.

• The last digit on the RPM/RCF/ROTOR display (0) flashes, indicating that you can enter the
RCF.

PN B29071AA 2-5
 Operation
Run Procedure

2 Press the s or t cursor key until the required RCF is displayed.

• The corresponding RPM is automatically calculated and the centrifuge runs at the
calculated speed.

Setting Run Time

Run time can be set for either a timed run or continuous operation.

Timed Run
Time can be set for up to 9 hours and 59 minutes (if the minutes parameter entered exceeds 59, it is
automatically converted into hours). During centrifugation, the TIME display begins counting down
when the rotor starts to spin, and continues the count-down until deceleration begins. The TIME
display shows the time remaining in the run in hours and minutes. When the time display reaches
zero, the run ends.

Continuous Run
If a run time of less than 1 minute or more than 9 hours and 59 minutes is selected, continuous
operation is activated. Time is not counted down during continuous operation; instead, the infinity
(¥) symbol, indicating continuous operation, lights up and time elapsed from the start of the run is
displayed. The run continues until the STOP key is pressed.

1 Press the TIME key.

The last digit on the TIME display flashes, indicating that the time can be entered with the
cursor keys.

2 Press the s or t cursor key until the required run duration is displayed.

Setting Acceleration Rate

The ACCEL key is used to select acceleration rates that protect delicate gradients. When the ACCEL
key is pressed, the ACC/DEC display flashes, indicating that one of the 10 preset acceleration rates
can be entered with the cursor keys. The selected rate depends on the type of run you are
performing. For pelleting runs, where sample mixing is not a concern, maximum acceleration
(setting 9) can be used. However, if delicate gradients are being run, a lower setting may be needed.
Acceleration rates are listed in Table 2.1.

1 Press the ACCEL key.

• The ACC/DEC display flashes, indicating that the selected rate number can be entered with
the cursor keys.

2-6 PN B29071AA
 Operation
Run Procedure 2

2 Press the s or t cursor key until the required number is displayed.

Table 2.1 Acceleration/Deceleration Times (in minutes:secondsa)

4700 RPM

Profile Accel Decel

9 0:38 0:24
8 0:37 0:32
7 0:45 0:52
6 1:23 1:28

5 2:08 2:11
4 2:47 2:50
3 4:22 4:24

2 8:43 8:44
1 11:37 11:38
0 17:27 coast
a. Times are approximate; actual
times vary depending on the
rotor load, run speed, and
voltage fluctuations.

Setting Deceleration Rate

The DECEL key is used to select deceleration rates that maintain optimum separation. When the
DECEL key is pressed, the ACC/DEC display flashes, indicating that one of the ten preset deceleration
rates can be entered with the cursor keys. The selected rate depends on the type of run you are
performing. For pelleting runs, where sample mixing is not a concern, maximum brake (setting 9)
can be used. However, if delicate gradients are being run, a lower brake setting may be needed.
Deceleration rates are listed in Table 2.1.

1 Press the DECEL key.

The ACC/DEC display flashes, indicating that the selected number can be entered with the
cursor keys.

2 Press the s or t cursor key until the required number is displayed.

Starting a Run
The run can be started using the parameters in memory from a previous run, or using new or
changed parameters that you enter using the procedure described above.

PN B29071AA 2-7
 Operation
Run Procedure

1 Check that all parameters are correct and the door is shut and latched.

2 Press the ENTER key, then the START key.

• The instrument displays the rotor speed as it accelerates, until it reaches and displays the
speed entered.
• If the actual speed exceeds the rotor’s maximum permitted speed of 4700 RPM, an error is
displayed and the centrifuge shuts down.
— See CHAPTER 3, Troubleshooting for information on error codes.
— The error must be cleared and an appropriate speed entered before the centrifuge
can be started.
— Throughout the run, checks are made to ensure that the rotor does not exceed
set speed.

• The RPM/RCF/ROTOR display indicates the rotor speed in RPM.

— (The RCF can be checked by pressing the RCF key.)

• A blinking LED at the bottom of the TIME display indicates that the run is in progress.
— This display also shows the time remaining in the run (or ¥ and elapsed time for
continuous operation).

WARNING
Risk of personal injury. Do not attempt to override the door interlock system while
the rotor is spinning or if there is any sound or vibration coming from the drive.
Always wait for the rotor to come to a complete stop before attempting to open
the centrifuge door.

CAUTION
Risk of personal injury or equipment damage. Do not lift or move the centrifuge
while the rotor is spinning. Do not place items on the centrifuge during operation.

Pulse Function
The pulse function, accessed by pressing the PULSE key, is used for short-duration runs.

• When the PULSE key is pressed, the rotor accelerates at maximum rate to the set speed and
continues to spin as long as the PULSE key is pressed. (The current run time, acceleration, and
deceleration settings are overridden by the pulse function.) When the PULSE key is released,
the rotor begins decelerating to 0 RPM using maximum deceleration. Using the pulse function
eliminates the need to press the START and STOP keys.

2-8 PN B29071AA
 Operation
Run Procedure 2

• When the PULSE key is pressed, the TIME display begins displaying the elapsed seconds. When
the PULSE key is released, the seconds stop accumulating. The ACCEL/DECEL display continues
to show the user-entered settings during a pulse run even though the maximum rates are used.
• If the PULSE key is pressed while the rotor is running at set speed, the rotor continues running
at speed until the PULSE key is released. When the PULSE key is released, the rotor begins
decelerating to 0 RPM using maximum deceleration.
• The centrifuge memory retains the parameters of the last run performed before the PULSE key
was pressed. At the end of a pulse run, after the centrifuge door is opened and closed the
previous run parameters are displayed.

Changing Parameters During a Run

While a run is in progress, run parameters (speed, time, and acceleration or deceleration rate) can
be altered without stopping the run. Run duration can also be changed from continuous to a
specified time period, or from a specified time period to continuous.

NOTE The deceleration rate cannot be changed after deceleration starts.

Use the program keys as described under Entering Run Parameters (above) to change parameters.
Parameter changes made during a run must be verified by pressing the ENTER key. For example, to
change run speed during centrifugation:

1 Press the RPM key.

• The last digit on the RPM/RCF/ROTOR display flashes, indicating that the RPM can be raised
or lowered with the cursor keys.

2 Press the s or t cursor key until the required RPM is displayed.

3 Press the ENTER key.

• The current RPM value is displayed, changing to the new value as the rotor accelerates or
decelerates to the new speed selected.
— The corresponding RCF is automatically calculated by the centrifuge.

Stopping a Run
A timed run ends automatically when the TIME display counts down to zero. The centrifuge emits a
series of audible tones when the rotor reaches 0 RPM. (Instructions for disabling the audible tones
are on page 2-10, Enabling or Disabling the Audible Tones.) To end a run in progress for any reason:

1 Press the STOP key for normal deceleration as selected by the deceleration profile.
(or)

PN B29071AA 2-9
 Operation
Unloading

Press and hold the STOP key for deceleration at the maximum rate (see Table 2.1).

NOTE If you hold the STOP key for at least two seconds, the deceleration process cannot be interrupted;
the centrifuge cannot be restarted until the rotor comes to a complete stop and the door is opened
and closed.

2 After the rotor stops spinning and the OPEN DOOR light comes on, press the OPEN DOOR key to
release the door latches, then open the door.

Unloading

NOTE When you remove the rotor, make sure that the tapered sleeve from the centrifuge drive shaft
does not come out with the rotor. If the tapered sleeve is inside the rotor drive hole, call Beckman Coulter
Field Service (1-800-742-2345 in the United States; outside the U.S. contact your local Beckman Coulter
office or visit us at www.beckmancoulter.com).

After completing a run, unload the rotor following the instructions in the rotor manual.

CAUTION
Risk of personal injury or contamination. If disassembly reveals evidence of
leakage, you should assume that some fluid escaped the rotor. Apply appropriate
decontamination procedures to the centrifuge and accessories according to the
procedures outlined by your laboratory safety officer.

Enabling or Disabling the Audible Tones

The audible tones (beeps) that sound at the end of each run and if an error occurs can be turned on
and off by following the steps below (rotor must be at 0 RPM).

1 Press the STOP key and hold for 3 seconds until the word “Beep” appears in the display.

2 Press either the s or t cursor key to turn the beeps on or off.

• (The word “on” or “off” is displayed to indicate the current setting.
— The arrow keys toggle between on and off settings.)

3 Press ENTER to save the selection.

2-10 PN B29071AA
 Operation
Summary of Run Procedures 2

Summary of Run Procedures

1 Press the POWER switch to on (I).

a. Open the centrifuge door (press the OPEN DOOR key and lift the door up).

2 Make sure that the tapered sleeve is in place at the base of the centrifuge drive shaft before
installing the rotor.
• The rotor does not operate properly if the sleeve is missing.

3 Install the rotor according to the instructions in the rotor manual.

• Always run the rotor with a balanced load.

4 Close the centrifuge door and push firmly down on it until you hear the latch engage.

5 Enter run parameters:

a. Set run speed — RPM, s or t; or RCF, s or t
b. Set run duration — TIME, s or t
c. Select acceleration rate (0 through 9)— ACCEL, s or t
d. Select deceleration rate (0 through 9)— DECEL, s or t

6 Check that all parameters are correct and the door is shut and latched, then press ENTER,
then START.

WARNING
Risk of personal injury. Do not attempt to override the door interlock system while
the rotor is spinning or if there is any sound or vibration coming from the drive.
Always wait for the rotor to come to a complete stop before attempting to open
the centrifuge door.

CAUTION
Risk of personal injury or equipment damage. Do not lift or move the centrifuge
while the rotor is spinning. Do not place items on the centrifuge during operation.

7 Wait for the set time to count down to zero, or end the run by pressing or holding the STOP key.

8 After the rotor stops spinning and the OPEN DOOR light comes on, press the OPEN DOOR key to
release the door latch; open the door.

PN B29071AA 2-11
 Operation
Summary of Run Procedures

9 Unload the rotor according to instructions in the rotor manual.

CAUTION
Risk of personal injury or contamination. If disassembly reveals evidence of
leakage, you should assume that some fluid escaped the rotor. Apply appropriate
decontamination procedures to the centrifuge and accessories according to the
procedures outlined by your laboratory safety officer.

2-12 PN B29071AA
 CHAPTER 3

Troubleshooting

Introduction

This section lists possible malfunctions, together with probable causes and corrective actions
required. Maintenance procedures are contained in CHAPTER 4, Care and Maintenance. For any
problems not covered here contact your Beckman Coulter representative.

NOTE It is your responsibility to decontaminate the centrifuge, as well as any rotors and accessories, before
requesting service by Beckman Coulter.

User Messages

CAUTION
If the message SEr appears on the display, do not press any keys while the
message is displayed. Turn the centrifuge power off (O) and back on (I) to clear the
message. This message indicates that you have inadvertently accessed the
service mode. Pressing any keys while in this mode could erase the centrifuge
memory and critically interfere with future operation.

If a problem occurs during operation, the rotor decelerates to a stop, an error code appears on the
RPM/RCF/ROTOR display, and the centrifuge emits a series of audible tones to alert the operator to
the error condition. Such problems may result from incorrect input or from an equipment
malfunction. Refer to Table 3.1 to determine the nature of the problem and recommended actions.
If you are unable to correct the problem, call your Beckman Coulter representative. To help
diagnose and correct the problem, gather as much information about the situation as you can:

• Write down the error number that appears on the display.

• Note the operating situation when the error occurred (speed, load type, etc.).
• Note any unusual environmental and/or operating conditions (ambient temperature, voltage
fluctuations, etc.).
• Add any other information that may be helpful.

NOTE Instructions for disabling the audible tones are in CHAPTER 2, Operation.

PN B29071AA 3-1
 Troubleshooting
User Messages

Table 3.1 Error Message Charta

Error Number Error Type Recommended Action Note

1-9 System Error • Allow to slow down All these errors stop the
• Power off/on centrifuge or cause it to
decelerate brakeless
10-19 Speedometer error • Allow to slow down
• Power off/on
20-29 Motor error • Power off
• Ensure ventilation
30-39 EEPROM error • Allow to slow down With error 34, 35, and 36, the
• Power off/on centrifuge will stop; with error 37
and 38 only an error message will
be given
46-49 Imbalance error • Allow to slow down
• Power off
• Eliminate the
imbalance
50-59 Door error • Press door key With error 50 and 51, the
• Close door centrifuge will stop
• Remove foreign
matter from the
opening of the door
lock device
60-69 Process error • Allow to slow down With error 60 message "power
• Power off/on failure during run", with error 61,
the message "stop after power
on"
70-79 Communication error • Allow to slow down
• Power off/on
80-89 Parameter error • Power off With error 83, error message only
• Allow to cool down
• Provide for better
ventilation
90-99 Other errors • Check connections
a. If the recommended action does not correct the problem, call your Beckman Representative.

3-2 PN B29071AA
 Troubleshooting
Other Possible Problems 3

Other Possible Problems

Possible malfunctions that may not be indicated by diagnostic messages are described in Table 3.2,
along with probable causes and corrective actions required. Possible causes for each problem are
listed in the probable order of occurrence. Perform the recommended corrective action in
sequence, as listed. If you are unable to correct the problem, call your Beckman Coulter
representative.

Table 3.2 Troubleshooting Chart

Problem Problem/Result Recommended Action

Imbalance LED lights 1. Rotor is out of balance 1. Check to be sure the rotor is in good condition and
and rotor decelerates is loaded symmetrically around the center of
to stop rotation, with containers of equal weight and
density opposite each other.
2. Centrifuge is misaligned (tilted) 2. Align the centrifuge on the bench or table.
3. Centrifuge was moved during 3. After the rotor comes to a complete stop, open and
operation close the centrifuge door, then restart.
4. Drive error (mechanical damage) 4. Call your Beckman Coulter representative.

Rotor cannot achieve 1. Line voltage below rating 1. Have a qualified service person measure line
set speed voltage while the instrument is operating.
2. Electrical failure 2. Make sure both ends of the power cord are securely
connected; call your Beckman Coulter
representative.
3. Motor failure 3. Call your Beckman Coulter representative.

Door does not open 1. Rotor spinning 1. Wait until the rotor stops.
2. Power not on 2. Plug in the power cord; turn power on (I).
3. Source power failure 3. See Emergency Door Release below.
4. Latch stuck 4. See Emergency Door Release below.

Displays are blank 1. Power not on 1. Plug in the power cord; turn power on (I).
2. Electrical failure 2. Make sure both ends of the power cord are securely
connected; call your Beckman Coulter
representative.
3. Fuse blown 3. System fuses cannot be replaced by the user. Call
your Beckman Coulter Representative.

Emergency Door Release

If the facility power fails only momentarily, the centrifuge resumes operation when power is
restored and the rotor returns to set speed. However, if the rotor comes to a complete stop you have

PN B29071AA 3-3
 Troubleshooting
Emergency Door Release

to restart the run when the power is restored. In the event of an extended power failure, you may
have to trip the door-locking mechanism manually to remove the rotor and retrieve your sample.

WARNING
Risk of personal injury. Any maintenance procedure requiring removal of a panel
exposes the operator to the possibility of electrical shock and/or mechanical
injury. Turn the power off and disconnect the instrument from the main power
source by removing the Mains (power) plug from the outlet receptacle, and refer
such maintenance to qualified service personnel.

1 Turn the Main power switch to off (O) and disconnect the power cord from the main power
source by removing its power plug from the receptacle.

WARNING
Risk of personal injury. Do not attempt to override the door interlock system while
the rotor is spinning or if there is any sound or vibration coming from the drive.
Always wait for the rotor to come to a complete stop before attempting to open
the centrifuge door.

2 Make sure that the rotor is not spinning.

3 Using a flat blade screwdriver, remove the plug from the opening on the left side of the control
panel (Figure 3.1).

Figure 3.1 Location of the emergency door release. opening.

1. Emergency Release access opening

4 Insert the supplied 5mm T-handle Allen wrench (P/N B31161) wrench horizontally into the
hole (Figure 3.2). The wrench will be guided through a funnel-shaped tube to the shaft of the
door lock motor.

3-4 PN B29071AA
 Troubleshooting
Emergency Door Release 3

Figure 3.2 Insertion of Allen wrench.

5 Turn the Allen wrench counter-clockwise to unlock the door.

WARNING
Risk of personal injury. Do not unlock or open the door unless the rotor is at a
complete stop.

DANGER
Risk of personal injury. Never try to slow or stop the rotor by hand. Always allow
the rotor to come to a complete stop before performing any action inside the rotor
chamber.

6 Remove the Allen wrench and replace the plug.

PN B29071AA 3-5
 Troubleshooting
Emergency Door Release

3-6 PN B29071AA
 CHAPTER 4

Care and Maintenance

Introduction

For maintenance not covered in this manual, contact your Beckman Coulter representative. User messages
are discussed in CHAPTER 3, Troubleshooting. Refer to the rotor manual and to Chemical Resistances
(publication IN-175) for instructions on the care of rotors and their accessories.

NOTE It is your responsibility to decontaminate the centrifuge, as well as any rotors and accessories, before
requesting service by Beckman Coulter Field Service.

WARNING
Risk of personal injury. Any maintenance procedure or servicing of this equipment
that requires removal of any covers can expose parts which involve the risk of
electric shock or personal injury. Make sure that the power switch is off (O) and
the centrifuge is disconnected from the main power source by removing the Mains
(power) plug from the outlet receptacle, and refer such servicing to qualified
service personnel.

CAUTION
Risk of personal injury. Flammability hazard. Do not use Ethanol or other
flammable liquids in or near operating centrifuges.

Maintenance

Preventive Maintenance
The following procedures should be performed regularly to ensure continued performance and
long service life of the centrifuge.

1 Regularly inspect the interior of the rotor chamber for accumulations of sample, dust, or glass
particles from broken sample tubes.

PN B29071AA 4-1
 Care and Maintenance
Cleaning

a. Clean as required (see Cleaning, below), as these accumulations can result in rotor
vibrations.

2 Regularly check the air intake and exhaust vents for obstructions.
a. Keep vents clear and clean.

3 To prevent the rotor from sticking, lubricate the drive shaft with Spinkote at least once a
month, and after each cleaning.

Cleaning

Frequent cleaning is recommended to prolong the life of the centrifuge. Always clean up spills when
they occur to prevent corrosives or contaminants from drying on component surfaces.

NOTE Before using any cleaning or decontamination methods, except those recommended by the
manufacturer, users should check with the manufacturer that the proposed methods will not damage the
equipment.

1 To prevent accumulations of sample, dust, and/or glass particles from broken sample tubes,
keep the interior of the rotor chamber clean and dry by frequent wiping with a cloth or
paper towel.

2 Clean the drive shaft, shaft cavity, threads, and the tie-down screw at least once a week using a
mild detergent such as Solution 555 and a soft brush.
a. Dilute the detergent with water (10 parts water to 1 part detergent).
b. Rinse thoroughly and dry completely.
c. Lubricate the drive shaft with Spinkote after cleaning.

3 Wash the bowl using a mild detergent such as diluted Solution 555.
a. Rinse thoroughly and dry completely.
b. If a cleaning solution other than Solution 555 is used, consult Chemical Resistances
(publication IN-175) or contact the cleaning-solution vendor to verify that the solution
will not damage the centrifuge.

4 Clean the centrifuge case and door by wiping with a cloth dampened with diluted Solution 555.
Do not use acetone or other solvents.

4-2 PN B29071AA
 Care and Maintenance
Tube Breakage 4

Tube Breakage

If a glass tube breaks, and all the glass is not contained in the bucket or rotor, you must thoroughly
clean the interior of the chamber bowl.

WARNING
Risk of personal injury. In the event of tube breakage, sharp glass fragments may
be embedded in the surfaces of the sealing gasket or chamber. Be careful when
examining or cleaning the sealing gasket or chamber.

1 Examine the door gasket to make sure that no glass particles are retained in it.
a. Carefully remove any glass particles that may remain.

2 Carefully wipe away any glass particles that remain in the bowl.

Decontamination

If the centrifuge and/or accessories are contaminated with radioactive or pathogenic solutions,
perform appropriate decontamination procedures. Refer to Chemical Resistances (IN-175) to be sure
the decontamination method will not damage any part of the centrifuge.

Sterilization and Disinfection

The centrifuge is finished with urethane paint. Ethanol (70%) may be used on this surface. See
Chemical Resistances for more information regarding chemical resistance of centrifuge and
accessory materials.

CAUTION
Risk of personal injury. Flammability hazard. Do not use Ethanol or other
flammable liquids in or near operating centrifuges.

While Beckman Coulter has tested these methods and found that they do not damage the
centrifuge, no guarantee of sterility or disinfection is expressed or implied. When sterilization or
disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

PN B29071AA 4-3
 Care and Maintenance
Storage and Transport

Storage and Transport

Storage
Before storing a centrifuge for an extended period, return it to its original shipping container to
protect it from dust and dirt. Reinsert the shipping foam (removed at time of centrifuge
installation) into the chamber, making sure the drive shaft is stabilized in the hole in the foam.

Temperature and humidity conditions for storage should meet the environmental requirements
described in the specifications given in CHAPTER 1, Description.

Notes on Transport
• Install the Transport Safety Device (see Transport Safety Device)
• Always lift the centrifuge with a lifting device

Transport Safety Device

The Transport Safety Device consists of two Allen screws, located on the bottom of the instrument.

Installation

1 Lift the centrifuge from the front of unit and tilt it backward to expose the bottom of the unit.

2 Stabilize the centrifuge by placing a suitable object, such as a wooden block under the
centrifuge.

3 Insert the two Allen Screws that were removed during the initial instrument installation (see
APPENDIX A, Transportation Safety Devices) into the holes in the bottom of the centrifuge. Align
the holes in the motor to allow the screws to engage the motor.

4 Using a #4 Allen wrench, tighten the two Allen screws in a clockwise direction to secure the
motor.

4-4 PN B29071AA
 Care and Maintenance
Storage and Transport 4

Figure 4.1 Locking Screws (Transport Safety Device)

1. Locking Screws

Returning a Centrifuge
Before returning a centrifuge or accessory for any reason, prior permission must be obtained from
Beckman Coulter, Inc. Contact your local Beckman Coulter office to obtain the authorization form
and for packaging and shipping instructions.

To protect our personnel, it is the customer’s responsibility to ensure that all parts are free from
pathogens and/or radioactivity. Sterilization and decontamination must be done before returning
the parts.

All parts must be accompanied by a signed note, plainly visible on the outside of the box, stating that they
are safe to handle and that they are not contaminated with pathogens or radioactivity. Failure to attach
this notification will result in return or disposal of the items without review of the reported problem.

Install the Transport Safety Device (see Transport Safety Device), and if possible, return in the
original packaging.

PN B29071AA 4-5
 Care and Maintenance
Supply List

Supply List

Contact your Beckman Coulter representative or visit www.beckman.com for information about
ordering parts, supplies, and publications. For your convenience, a partial list is given below.

Refer to the applicable rotor manual for materials and supplies needed for rotors.

Replacement Parts
NOTE For MSDS information, go to the Beckman Coulter website at www.beckmancoulter.com.

Description Part Number

Rotor tie-down screw 368245

T handle rotor wrench, size 13 368246
Allen T wrench, size 5 B31161
emergency door release
Spinkote 306812

4-6 PN B29071AA
 APPENDIX A

Installation

Introduction

This chapter contains instructions for installing and connecting the centrifuge. Check that required
clearances and electrical power are available.

WARNING
Risk of personal injury or equipment damage. This centrifuge weigh 77kg/169 lbs.
DO NOT attempt to lift or move it without assistance from a lifting device and/or
another person.

Installing the Instrument

WARNING
Risk of personal injury or equipment damage. Do not place the centrifuge in or
near areas containing flammable reagents or combustible fluids. Vapors from
these materials could enter the centrifuge air system and be ignited by the motor.

WARNING
Risk of personal injury. Maintain a 30 cm (12 in.) clearance envelope around the
centrifuge while it is running. During operation you should come within the 30 cm
(12 in.) clearance envelope only to adjust the instrument controls, if necessary.
Do not handle or store hazardous materials within the 30 cm (12 in.) area
surrounding the centrifuge.

1 The centrifuge ships in a wooden box on a wooden pallet. For easy access, remove the top of the
box, the foam insert on top of the centrifuge, and then the upper part (sides) of the box and set
them aside.
a. Then, with the help of another person and/or a lifting device, move the centrifuge from the
pallet to its final position.
• (Note the warning above regarding centrifuge weight.)

PN B29071AA A-1
 Installation
Transportation Safety Devices

2 Position the centrifuge on a level surface, such as a sturdy table or laboratory bench that is able
to support the weight of the centrifuge and resist vibration.
• Refer to CHAPTER 1, Specifications for weight.

a. Make sure that the centrifuge front feet are fully supported on the table.
b. Locate the centrifuge in an area with sufficient ventilation to allow for heat dissipation.
c. Check that there are 7.6-cm (3-in.) clearances at the sides of the centrifuge to ensure
sufficient air circulation.
Dimensions are shown in Figure A.2. Additional clearance is required on the right side to
allow access to the power switch.
The centrifuge must have adequate air ventilation to ensure compliance to local
requirements for vapors produced during operation.
Ambient temperatures during operation should not be lower than 5°C (41°F) or higher than
40°C (104°F). Relative humidity should not exceed 80% (non condensing).

NOTE During transport between areas with varying temperatures, condensation may occur inside
the centrifuge. Allow sufficient drying time before running the centrifuge.

Transportation Safety Devices

A sheet of foam rubber is installed in the rotor chamber at the factory. A hole in the center of the
foam stabilizes the drive shaft during transport. On receipt of the centrifuge, remove the foam and
store it in case future relocation of the centrifuge is necessary.

Transport Safety Device

The Transport Safety Device consists of two Allen screws, located on the bottom of the instrument.
These screws lock the rotor in place and must be removed prior to operating the centrifuge.

Removal

1 Lift the centrifuge at the front of unit and tilt it backward to expose the bottom of the unit.

2 Stabilize the centrifuge by placing a suitable object, such as a wooden block, under the
centrifuge.

3 Using a #4 Allen wrench, turn the two Allen screws counter-clockwise to remove them.

A-2 PN B29071AA
 Installation
Electrical Requirements A

Figure A.1 Locking Screws (Transport Safety Device)

1. Locking Screws

4 Save the Transport Safety Device Screws for future transport of the centrifuge.

Electrical Requirements

See the CHAPTER 1, Specifications for centrifuge electrical requirements.

Make sure the voltage and frequency imprinted on the name rating plate affixed to the right side of
the centrifuge agree with the line voltage and frequency of the outlet used. Plug in both ends of the
centrifuge power cord. If there is any question about voltage, have a qualified service person
measure it under load while the drive is operating.

A 1.8-m (6-ft) power cord with grounded plug is supplied with the centrifuge. Make sure that the
matching wall outlet is located near the centrifuge and is easily accessible.

NOTE The power plug serves as the Disconnecting Device and must remain easily accessible.

PN B29071AA A-3
 Installation
Electrical Requirements

Figure A.2 Dimensions of the Centrifuge

NOTE Additional clearance is required on the right side to allow to allow access to the power switch.

WARNING
Risk of personal injury. Improper electrical connection could produce an electric
shock. To reduce the risk of electrical shock, this equipment uses a three-wire
electrical cord and plug to connect the centrifuge to earth-ground. To preserve
this safety feature:
• Make sure that the matching wall outlet receptacle is properly wired and
earth-grounded. Check that the line voltage agrees with the voltage listed on
the name rating plate affixed to the centrifuge.
• Never use a three-to-two wire plug adapter.
• Never use a two-wire extension cord or a two-wire non-grounding type of
multiple-outlet receptacle strip.

A-4 PN B29071AA
 Installation
Test Run A

Test Run

NOTE The centrifuge must be plugged in and the power switch turned to on position (I) before the door
can be opened.

Beckman Coulter recommends that you make a test run to ensure that the centrifuge is in proper
operating condition following shipment. See CHAPTER 2, Operation for instructions on operating
the centrifuge.

After completing the test run, log on to www.beckmancoulter.com to register your centrifuge. This
validates the centrifuge warranty and ensures your receipt of further information regarding new
accessories and/or modifications as they become available.

PN B29071AA A-5
 Installation
Test Run

A-6 PN B29071AA
 APPENDIX B

Table of Hazardous Substance’s

Name and Concentration

This materials declaration table (the Table of Hazardous Susbtance’s Name and Concentration) and
the China RoHS Caution Label are to meet People's Republic of China Electronic Industry Standard
SJ/T11364-2006”Marking for Control of Pollution Caused by Electronic Information Products”
requirements.

PN B29071AA B-1
B-2

ᴹ∂ᴹᇣ⢙䍘਽〠৺ਜ਼䟿Ⲵḷ䇶Ṭᔿ

Table of Hazardous Substance’s Name and Concentration

Table of Hazardous Substance’s Name and Concentration

⭥ᆀؑ᚟ӗ૱ਧ⸱ EIP Part Numbers: B30585, B30588 ӗ૱਽〠 Product Name: Benchtop Centrifuge
ӗ૱රਧProduct Model Number: Allegra X-5

䜘Ԧ਽〠 ᴹ∂ᴹᇣ⢙䍘ᡆ‫ݳ‬㍐
Component Name Hazardous Substances Name

䫵 ⊎ 䭹 ‫ޝ‬ԧ䬜 ཊⓤ㚄㤟 ཊⓤҼ㤟䟊

Pb Hg Cd Cr6+ PBBB PBDE

ঠࡧ⭥䐟ᶯ㓴Ԧ Printed Circuit Board Sub Assemblies O O O O O O

⭥㔶㓴Ԧ Cable Sub Assemblies O O O O O O
ກᯉ䜘Ԧ Plastic Parts O O O O O O
䫓䠁䜘Ԧ Sheet Metal O O O O O O
䘎᧕䜘Ԧ ˄㷪эˈ㷪ṃㅹ˅ Hardware (screws, bolts, etc.) O O O O O O
⭥Ⓚ㓴Ԧ Power Supply Sub Assembly O O O O O O
O: 㺘⽪䈕ᴹ∂ᴹᇣ⢙䍘൘䈕䜘Ԧᡰᴹ൷䍘ᶀᯉѝⲴਜ਼䟿൷൘ SJ/T 11363-2006 ḷ߶㿴ᇊⲴ䲀䟿㾱≲ԕл
X: 㺘⽪䈕ᴹ∂ᴹᇣ⢙䍘㠣ቁ൘䈕䜘ԦⲴḀа൷䍘ᶀᯉѝⲴਜ਼䟿䎵ࠪ SJ/T 11363-2006 ḷ߶㿴ᇊⲴ䲀䟿㾱≲
˄Աъਟ൘↔༴ˈṩᦞᇎ䱵ᛵߥሩк㺘ѝᢃ“X”Ⲵᢰᵟ৏ഐ䘋㹼䘋а↕䈤᰾˅
O: Indicates that the toxic or hazardous substances contained in all of the homogeneous materials for this part is below the limit requirements
in SJ/T 11363-2006.
X: Indicates that the toxic or hazardous substances contained in at least one of the homogeneous materials used in this part is above the limit requirements
in SJ/T 11363-2006.
(Enterprise may further provide in this box technical explanation for marking “X” based on their actual conditions).
PN B29071AA
 Beckman Coulter, Inc.
Allegra X-5 Centrifuge Warranty
Subject to the exceptions and upon the conditions specified below, Beckman Coulter agrees to correct, either
by repair, or, at its election, by replacement, any defects of material or workmanship which develop within
one (1) year after delivery of the Allegra X-5 Centrifuge (the product), to the original Buyer by Beckman
Coulter or by an authorized representative provided that investigation and factory inspection by Beckman
Coulter discloses that such defect developed under normal and proper use.

Some components and accessories by their nature are not intended to and will not function for as long as one
(1) year. A complete list of such components or accessories is maintained at the factory and at each
Beckman Coulter District Sales Office. The lists applicable to the products sold hereunder shall be deemed to
be part of this warranty. If any such component or accessory fails to give reasonable service for a reasonable
period of time, Beckman Coulter will repair or, at its election, replace such component or accessory. What
constitutes either reasonable service and a reasonable period of time shall be determined solely by
Beckman Coulter.

Replacement
Any product claimed to be defective must, if requested by Beckman Coulter, be returned to the factory,
transportation charges prepaid, and will be returned to Buyer with the transportation charges collect unless
the product is found to be defective, in which case Beckman Coulter will pay all transportation charges.

Conditions
Beckman Coulter shall be released from all obligations under all warranties either expressed or implied, if the
product(s) covered hereby are repaired or modified by persons other than its own authorized service
personnel, unless such repair in the sole opinion of Beckman Coulter is minor, or unless such modification is
merely the installation of a new Beckman Coulter plug-in component for such product(s).

Disclaimer
IT IS EXPRESSLY AGREED THAT THE ABOVE WARRANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS
AND OF THE WARRANTY OF MERCHANTABILITY AND THAT NEITHER BECKMAN COULTER, INC. NOR ITS
SUPPLIERS SHALL HAVE ANY LIABILITY FOR SPECIAL OR CONSEQUENTIAL DAMAGES OF ANY KIND
WHATSOEVER ARISING OUT OF THE MANUFACTURE, USE, SALE, HANDLING, REPAIR, MAINTENANCE, OR
REPLACEMENT OF THE PRODUCT.

PN B29071AA Warranty-1
 Beckman Coulter, Inc. Allegra X-5 Centrifuge Warranty

Warranty-2 PN B29071AA
 English / Deutsch / Español / Français / Italiano / Portugués / Русский / 中文 / 日本語 / 국어

Symbol Simbole
Symbol символ
Simbolo 符号 Title / Titel / Titulo / Titre / Titolo / Titulo / Название / 标题 / タイトル / 제목
Symbole 記号
Simbolo 상징
Dangerous voltage Tensão perigosa
Gefährliche electrische Spannung Опасное напряжение тока
Voltaje peligroso 危险电压
Courant haute tension 危険な電圧
Pericolo: alta tensione 위 전압
Caution, consult accompanying documents Cuidado, ulta documentos adjuntos
Vorsicht, konsultieren Begleitdokumente Внимание, советует с сопроводительными документами
Atención, consulta documentos adjuntos 注意, 咨询附属单证
Attention, consultent des documents d'accompagnement 注意, 伴う文書に相談しなさい
Attenzione, consulta i documenti di accompagnamento 주의, 동반 문서를 상담+십시오
Biohazard Material infeccioso potencial
Potentiell infektiösem Material биологической опасности
Riesgo biológico 可能的传染性物
Risque biologiqu 潜在的な感染性物質
Pericolo biologico 전염+는 물자
On (power) Fora (o poder)
Ein (Netzverbindung) На (мощности)
Encendido 开 ?电源A
Marche (mise sous tension) ン ?電源A
Acceso (sotto tensione) 에 (E)
Off (power) Fora de (poder)
Aus (Netzverbindung) С (сила)
Apagado ?电源A
Arrêt (mise sous tension) ン ?電源A
Spento (fuori tensione) 떨어져 (E)
Protective earth (ground) Terra de proteção (terra)
Schutzleiteranschluß Защитное заземление (земля)
Puesta a tierra de protección 保护接地
Liaison à la terre 保護アース ?接地A
Collegamento di protezione a terra 방어적인 지구 (지상)
Earth (ground) Terra
Erde (Masse) Земли
La tierra (suelo) 接地
Terre (sol) アース ?接地A
Scarica a terra 지구 (지상)
In vitro diagnostic medical device In vitro dispositivo médico diagnóstico
In-vitro-diagnosemedizinisches Gerät В медицинской службе диагностики vitro
Aparato médico de diagnóstico in vitro 体外诊断医疗设备
Appareil médical diagnostique in vitro 生体外の診断医療機器
Apparecchio medico diagnostico in vitro 생체외 진단 의료 기기
Manufacturer Fabricante
Hersteller производитель
Fabricante 制造商
Fabricant メーカー
Fabbricante 제조자
Authorized representative in the European Community Representante autorizado na Comunidade Européia
Autorisierter Repräsentant in der Europäischen Gemeinschaft Утверженный представитель в сообществ
Representante autorizado en la Comunidad Europea 在欧共体的授权代表
Représentant autorisé dans le Communauté européen 欧州共同体の承認された代表
Rappresentante autorizzato nella Comunità Europea 유럽 공동체에 있는 †가 대‰자
Consult Instructions for Use Consulte instruções para o uso
Konsultieren Sie Anwendungsvorschriften Советуйте с инструкциями для пользы
Consulte las instrucciones para el uso 咨询使用说明书
Consultez les instructions pour l'usage 使用説明に相談しなさい
Consulti le istruzioni per uso 사용 설명을 상담+십시오
 Related Documents
SX4700 Swinging-Bucket Rotor Instructions
for Use
PN B30343

Chemical Resistances for Beckman Coulter

Centrifugation Products
PN IN-175

Use and Care of Tubes and Bottles

PN IN-192

Available in hard copy or electronic pdf by

request.

Available at www.beckmancoulter.com

www.beckmancoulter.com

© 2013 Beckman Coulter, Inc.

All Rights Reserved