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3) The idea of spontaneous generation postulated that
A) organisms could evolve into the next generation of organisms.
B) organisms could spontaneously turn into other types of organisms.
C) living organisms could spontaneously arise from non-living material.
D) living organisms could spontaneously arise from other living organisms.
E) living organisms must contain at least ten cells.

Answer: C
Section: 01.01
Topic: History of Microbiology
Bloom's: 2. Understand
Learning Outcome: 01.01 Describe the key experiments of scientists who disproved spontaneous
generation.
ASM Objective: 02.01 The structure and function of microorganisms have been revealed by the
use of microscopy (including bright field, phase contrast, fluorescent, and electron).

4) Which of these scientists were involved in investigating the idea of spontaneous generation?
A) Redi and van Leeuwenhoek
B) Redi and Pasteur
C) van Leeuwenhoek and Pasteur
D) Pasteur and Escherich
E) Escherich and Redi

Answer: B
Section: 01.01
Topic: History of Microbiology
Bloom's: 2. Understand
Learning Outcome: 01.01 Describe the key experiments of scientists who disproved spontaneous
generation.
ASM Objective: 02.01 The structure and function of microorganisms have been revealed by the
use of microscopy (including bright field, phase contrast, fluorescent, and electron).

2
Copyright © 2019 McGraw-Hill Education. All rights reserved.
No reproduction or distribution without the prior written consent of McGraw-Hill Education.
5) The work of Tyndall and Cohn
A) supported the idea of spontaneous generation rather than the idea of biogenesis.
B) explained why some spontaneous generation investigators got different results from those of
Pasteur.
C) showed that all microbes caused disease if they enter the human body.
D) allowed scientists to see microorganisms using a simple microscope.
E) showed that boiling fails to kill vegetative bacteria.

Answer: B
Section: 01.01
Topic: History of Microbiology
Bloom's: 3. Apply
Learning Outcome: 01.01 Describe the key experiments of scientists who disproved spontaneous
generation.
ASM Objective: 02.03 Bacteria and Archaea have specialized structures (e.g., flagella,
endospores, and pili) that often confer critical capabilities.; 02.01 The structure and function of
microorganisms have been revealed by the use of microscopy (including bright field, phase
contrast, fluorescent, and electron).

6) The structures present in the hay infusions used in experiments on spontaneous generation that
made them difficult to sterilize are
A) chloroplasts.
B) endospores.
C) organelles.
D) toxins.
E) nuclei.

Answer: B
Section: 01.01
Topic: History of Microbiology
Bloom's: 1. Remember
Learning Outcome: 01.01 Describe the key experiments of scientists who disproved spontaneous
generation.
ASM Objective: 02.03 Bacteria and Archaea have specialized structures (e.g., flagella,
endospores, and pili) that often confer critical capabilities.; 02.01 The structure and function of
microorganisms have been revealed by the use of microscopy (including bright field, phase
contrast, fluorescent, and electron).

3
Copyright © 2019 McGraw-Hill Education. All rights reserved.
No reproduction or distribution without the prior written consent of McGraw-Hill Education.
7) The contradictory results obtained by different scientists apparently doing the same experiments
in investigating spontaneous generation
A) show that experiments should only be done once.
B) show the importance of exactly duplicating experimental conditions.
C) led to further experiments that ultimately proved spontaneous generation.
D) could not be explained by anyone involved in the work.
E) led to the development of swan-necked flasks.

Answer: B
Section: 01.01
Topic: History of Microbiology
Bloom's: 3. Apply
Learning Outcome: 01.01 Describe the key experiments of scientists who disproved spontaneous
generation.; 01.03 Describe the scientific method, using Pasteur's swan-necked flask experiment
as an example.
ASM Objective: 02.03 Bacteria and Archaea have specialized structures (e.g., flagella,
endospores, and pili) that often confer critical capabilities.; 02.01 The structure and function of
microorganisms have been revealed by the use of microscopy (including bright field, phase
contrast, fluorescent, and electron).

8) If Pasteur had done his experiments investigating spontaneous generation in a horse stable,
A) the results would probably have supported the idea of spontaneous generation.
B) the results would probably not have supported the idea of spontaneous generation.
C) the results would probably been the same as those obtained in a laboratory.
D) the results would probably have supported the idea of spontaneous biogenesis.
E) it would probably have taken several years to obtain any results.

Answer: A
Section: 01.01
Topic: History of Microbiology; Prokaryotes
Bloom's: 3. Apply
Learning Outcome: 01.01 Describe the key experiments of scientists who disproved spontaneous
generation.; 01.03 Describe the scientific method, using Pasteur's swan-necked flask experiment
as an example.
ASM Objective: 02.03 Bacteria and Archaea have specialized structures (e.g., flagella,
endospores, and pili) that often confer critical capabilities.; 02.01 The structure and function of
microorganisms have been revealed by the use of microscopy (including bright field, phase
contrast, fluorescent, and electron).

4
Copyright © 2019 McGraw-Hill Education. All rights reserved.
No reproduction or distribution without the prior written consent of McGraw-Hill Education.
9) Cellulose is a major component of plants and is only directly digested by
A) carnivores.
B) termites.
C) herbivores.
D) microorganisms.
E) birds.

Answer: D
Section: 01.02
Topic: Environmental Microbiology; Prokaryotes
Bloom's: 2. Understand
Learning Outcome: 01.02 Explain how the successful challenge to the idea of spontaneous
generation led to the Golden Age of Microbiology.
ASM Objective: 02.01 The structure and function of microorganisms have been revealed by the
use of microscopy (including bright field, phase contrast, fluorescent, and electron).; 03.03 The
survival and growth of any microorganism in a given environment depends on its metabolic
characteristics.; 05.04 Microorganisms, cellular and viral, can interact with both human and
nonhuman hosts in beneficial, neutral or detrimental ways.; 06.01 Microbes are essential for life as
we know it and the processes that support life (e.g., in biogeochemical cycles and plant and/or
animal microflora).

10) Plants are dependent on microorganisms for

A) providing oxygen in a usable form.
B) providing water.
C) changing atmospheric nitrogen to a usable form.
D) providing simple carbohydrates.
E) providing complex proteins.

Answer: C
Section: 01.02
Topic: Environmental Microbiology; Prokaryotes
Bloom's: 3. Apply
Learning Outcome: 01.04 Explain the importance of microorganisms in the health of humans and
the surrounding environment.
ASM Objective: 03.01 Bacteria and Archaea exhibit extensive, and often unique, metabolic
diversity (e.g., nitrogen fixation, methane production, anoxygenic photosynthesis).; 05.03
Microorganisms and their environment interact with and modify each other.; 05.04
Microorganisms, cellular and viral, can interact with both human and nonhuman hosts in
beneficial, neutral or detrimental ways.

5
Copyright © 2019 McGraw-Hill Education. All rights reserved.
No reproduction or distribution without the prior written consent of McGraw-Hill Education.
11) Microorganisms are involved in
A) causing disease.
B) curing/treating disease.
C) preparing food.
D) cleaning up pollutants.
E) All of the answer choices are correct.

Answer: E
Section: 01.02
Topic: Applied and Industrial Microbiology
Bloom's: 2. Understand
Learning Outcome: 01.04 Explain the importance of microorganisms in the health of humans and
the surrounding environment.; 01.05 List three commercial benefits of microorganisms.
ASM Objective: 03.03 The survival and growth of any microorganism in a given environment
depends on its metabolic characteristics.; 05.03 Microorganisms and their environment interact
with and modify each other.; 05.04 Microorganisms, cellular and viral, can interact with both
human and nonhuman hosts in beneficial, neutral or detrimental ways.; 06.03 Humans utilize and
harness microorganisms and their products.

12) Bacteria have been used to help produce or modify all of the following food products EXCEPT
A) cheeses.
B) beer and wine.
C) pickled products.
D) bread.
E) peanuts.

Answer: E
Section: 01.02
Topic: Applied and Industrial Microbiology
Bloom's: 1. Remember
Learning Outcome: 01.05 List three commercial benefits of microorganisms.
ASM Objective: 05.04 Microorganisms, cellular and viral, can interact with both human and
nonhuman hosts in beneficial, neutral or detrimental ways.; 06.03 Humans utilize and harness
microorganisms and their products.

6
Copyright © 2019 McGraw-Hill Education. All rights reserved.
No reproduction or distribution without the prior written consent of McGraw-Hill Education.
13) Microorganisms are involved in all of the following EXCEPT
A) production of medicinal products.
B) converting oxygen to a form useful to plants.
C) food production.
D) pollution cleanup.
E) converting nitrogen to a form useful to plants.

Answer: B
Section: 01.02
Topic: Applied and Industrial Microbiology
Bloom's: 3. Apply
Learning Outcome: 01.04 Explain the importance of microorganisms in the health of humans and
the surrounding environment.; 01.05 List three commercial benefits of microorganisms.
ASM Objective: 06.01 Microbes are essential for life as we know it and the processes that
support life (e.g., in biogeochemical cycles and plant and/or animal microflora).; 06.03 Humans
utilize and harness microorganisms and their products.

14) Bioremediation refers to

A) rehabilitating wayward bacteria.
B) using bacteria to clean up pollutants.
C) development of new vaccines.
D) monitoring newly discovered disease organisms.
E) destroying organisms causing emerging infectious diseases.

Answer: B
Section: 01.02
Topic: Applied and Industrial Microbiology
Bloom's: 2. Understand
Learning Outcome: 01.04 Explain the importance of microorganisms in the health of humans and
the surrounding environment.; 01.05 List three commercial benefits of microorganisms.
ASM Objective: 05.01 Microorganisms are ubiquitous and live in diverse and dynamic
ecosystems.; 05.04 Microorganisms, cellular and viral, can interact with both human and
nonhuman hosts in beneficial, neutral or detrimental ways.; 06.03 Humans utilize and harness
microorganisms and their products.

7
Copyright © 2019 McGraw-Hill Education. All rights reserved.
No reproduction or distribution without the prior written consent of McGraw-Hill Education.
15) Which of the following about the Golden Age of Medical Microbiology is FALSE?
A) It started with the development of the first microscopes.
B) It occurred during the late 1800s to the early 1900s.
C) It is a time when the knowledge of bacteria and work with them expanded.
D) It was during this time that people realized that diseases could be caused by invisible agents.
E) It was a time when several major advances were made in microbiology.

Answer: A
Section: 01.02
Topic: History of Microbiology
Bloom's: 3. Apply
Learning Outcome: 01.02 Explain how the successful challenge to the idea of spontaneous
generation led to the Golden Age of Microbiology.; 01.04 Explain the importance of
microorganisms in the health of humans and the surrounding environment.
ASM Objective: 05.04 Microorganisms, cellular and viral, can interact with both human and
nonhuman hosts in beneficial, neutral or detrimental ways.; 06.03 Humans utilize and harness
microorganisms and their products.

16) Which of the following statements about newly emerging or reemerging diseases is FALSE?
A) They may be caused by changing lifestyles.
B) Examples include Lyme disease and toxic shock syndrome.
C) They may result from a breakdown in sanitation and social order.
D) They are all caused by drug-resistant pathogens.
E) They may result when microbes evolve and develop new characteristics.

Answer: D
Section: 01.02
Topic: Infection and Disease
Bloom's: 2. Understand
Learning Outcome: 01.04 Explain the importance of microorganisms in the health of humans and
the surrounding environment.; 01.07 Describe the role of microbes in disease, including examples
of past triumphs and remaining challenges.
ASM Objective: 01.03 Human impact on the environment influences the evolution of
microorganisms (e.g., emerging diseases and the selection of antibiotic resistance).; 05.04
Microorganisms, cellular and viral, can interact with both human and nonhuman hosts in
beneficial, neutral or detrimental ways.

8
Copyright © 2019 McGraw-Hill Education. All rights reserved.
No reproduction or distribution without the prior written consent of McGraw-Hill Education.
17) Lyme disease is an example of a disease that is due to
A) increased interaction between humans and tick-carrying animals.
B) failure to effectively vaccinate children.
C) a mutation in the human genome.
D) climate change leading to a significantly greater mosquito population.
E) an increase in the number of people travelling to Asia and Africa.

Answer: A
Section: 01.02
Topic: Infection and Disease
Bloom's: 3. Apply
Learning Outcome: 01.04 Explain the importance of microorganisms in the health of humans and
the surrounding environment.; 01.07 Describe the role of microbes in disease, including examples
of past triumphs and remaining challenges.
ASM Objective: 01.03 Human impact on the environment influences the evolution of
microorganisms (e.g., emerging diseases and the selection of antibiotic resistance).; 05.04
Microorganisms, cellular and viral, can interact with both human and nonhuman hosts in
beneficial, neutral or detrimental ways.

18) The outbreak of measles within the last few years is due to
A) mutation of the measles virus.
B) change in the environment.
C) a decline in vaccination of children in the previous years.
D) increase in sensitivity of detection techniques.
E) emergence of novel measles viruses.

Answer: C
Section: 01.02
Topic: Infection and Disease
Bloom's: 2. Understand
Learning Outcome: 01.04 Explain the importance of microorganisms in the health of humans and
the surrounding environment.; 01.07 Describe the role of microbes in disease, including examples
of past triumphs and remaining challenges.
ASM Objective: 05.04 Microorganisms, cellular and viral, can interact with both human and
nonhuman hosts in beneficial, neutral or detrimental ways.

9
Copyright © 2019 McGraw-Hill Education. All rights reserved.
No reproduction or distribution without the prior written consent of McGraw-Hill Education.
19) Which of the statements regarding smallpox is TRUE?
A) Smallpox has been eliminated as a naturally occurring infection in human beings.
B) Smallpox still occasionally occurs in developing countries.
C) Smallpox outbreaks sometimes occur in chimpanzee populations.
D) Smallpox has been eliminated by effective vaccination.
E) Smallpox has been eliminated as a naturally occurring infection in human beings, AND has
been eliminated by effective vaccination.

Answer: E
Section: 01.02
Topic: History of Microbiology
Bloom's: 2. Understand
Learning Outcome: 01.04 Explain the importance of microorganisms in the health of humans and
the surrounding environment.; 01.07 Describe the role of microbes in disease, including examples
of past triumphs and remaining challenges.
ASM Objective: 05.04 Microorganisms, cellular and viral, can interact with both human and
nonhuman hosts in beneficial, neutral or detrimental ways.

20) Smallpox
A) has occurred in a few countries since 1977.
B) has little potential as a weapon of bioterrorism.
C) has not occurred anywhere in the word since 1977.
D) very seldom kills people, but does scar them.
E) is an emerging infectious disease.

Answer: C
Section: 01.02
Topic: History of Microbiology
Bloom's: 2. Understand
Learning Outcome: 01.04 Explain the importance of microorganisms in the health of humans and
the surrounding environment.; 01.07 Describe the role of microbes in disease, including examples
of past triumphs and remaining challenges.
ASM Objective: 05.04 Microorganisms, cellular and viral, can interact with both human and
nonhuman hosts in beneficial, neutral or detrimental ways.

10
Copyright © 2019 McGraw-Hill Education. All rights reserved.
No reproduction or distribution without the prior written consent of McGraw-Hill Education.
21) Ulcers, previously thought to be caused by stress, are in fact often caused by
A) a bacterial infection.
B) an insufficient diet.
C) a genetic mutation.
D) pathogenic normal microbiota.
E) a viral infection.

Answer: A
Section: 01.02
Topic: Infection and Disease
Bloom's: 2. Understand
Learning Outcome: 01.04 Explain the importance of microorganisms in the health of humans and
the surrounding environment.; 01.07 Describe the role of microbes in disease, including examples
of past triumphs and remaining challenges.
ASM Objective: 05.04 Microorganisms, cellular and viral, can interact with both human and
nonhuman hosts in beneficial, neutral or detrimental ways.

22) Bacteria are useful to study because

A) they produce protein in a simpler manner than more complex organisms.
B) they have the same fundamental metabolic and genetic properties as higher organisms.
C) they produce energy in a simpler manner than more complex organisms.
D) they are resistant to all known antibiotics.
E) they produce peptidoglycan in a simpler manner than more complex organisms.

Answer: B
Section: 01.03
Topic: Tools and Methods of Culturing, Classifying, and Identify Microorganisms
Bloom's: 2. Understand
Learning Outcome: 01.06 Describe why microorganisms are useful research tools.
ASM Objective: 04.02 Although the central dogma is universal in all cells, the processes of
replication, transcription, and translation differ in Bacteria, Archaea, and Eukaryotes.; 05.04
Microorganisms, cellular and viral, can interact with both human and nonhuman hosts in
beneficial, neutral or detrimental ways.

11
Copyright © 2019 McGraw-Hill Education. All rights reserved.
No reproduction or distribution without the prior written consent of McGraw-Hill Education.
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