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142 views111 pages

Test Bank For Microbiology An Introduction 12th Edition Tortora Funke Case 0073402354 9780073402352 Download

newest edition 2025

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Chapter 02 - Tools of the Laboratory: The Methods for Studying Microorganisms

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Chapter 02: Tools of the Laboratory: The Methods for Studying


Microorganisms
Multiple Choice Questions

1. The Five I's of studying microorganisms include all of the following except
A. inoculation.
B. incubation.
C. infection.
D. isolation.
E. identification.

ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I's mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

2-1
Chapter 02 - Tools of the Laboratory: The Methods for Studying Microorganisms

2. The term that refers to the purposeful addition of microorganisms into a laboratory nutrient
medium is
A. isolation.
B. inoculation.
C. immunization.
D. infection.
E. contamination.

ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I's mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

2-2
Chapter 02 - Tools of the Laboratory: The Methods for Studying Microorganisms

3. A pure culture contains


A. only one species of microorganism.
B. only bacteria.
C. a variety of microbes from one source.
D. a variety of species from the same genus.
E. None of the choices is correct.

ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I's mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

4. The correct microbiological term for the tiny sample of specimen that is put into a nutrient
medium in order to produce a culture is the
A. colony.
B. inoculum.
C. streak.
D. loop.
E. None of the choices is correct.

ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I's mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

5. Which of the following is essential for development of discrete, isolated colonies?


A. broth medium
B. differential medium
C. selective medium
D. solid medium
E. assay medium

ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

2-3
Chapter 02 - Tools of the Laboratory: The Methods for Studying Microorganisms

6. Which method often results in colonies developing down throughout the agar along with
some colonies on the surface?
A. streak plate
B. spread plate
C. pour plate
D. All of the choices are correct.
E. None of the choices is correct.

ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I's mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

7. What type of isolation technique is most effective for the majority of applications?
A. pour plate
B. streak plate
C. spread plate
D. loop dilution
E. culture plate

ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I's mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

8. Which of the following will result when 1% to 5% agar is added to nutrient broth, boiled
and cooled?
A. a pure culture B.
a mixed culture C.
a solid medium D.
a liquid medium
E. a contaminated medium

ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

2-4
Chapter 02 - Tools of the Laboratory: The Methods for Studying Microorganisms

9. Agar is an important component of media because


A. bacteria require agar to grow.
B. agar inhibits mold growth.
C. agar provides a solid surface for bacterial growth.
D. agar prevents contamination.
E. All of the choices are correct.

ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

10. The three physical forms of laboratory media are


A. solid, liquid, and gas.
B. solid, semisolid, and liquid.
C. streak plate, pour plate, and broth.
D. aerobic, anaerobic, and micro aerobic.
E. None of the choices is correct.

ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

11. Which of the following is not an inoculating tool?


A. petri dish
B. loop
C. needle
D. pipette
E. swab

ASM Objective: 08.05 Use appropriate microbiological and molecular lab equipment.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I's mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

2-5
Chapter 02 - Tools of the Laboratory: The Methods for Studying Microorganisms

12. Agar is a complex polysaccharide that comes from a/an


A. green plant.
B. fungus.
C. mold.
D. algae.
E. euglena.

ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

13. Which of the following is not a benefit of agar as a solid medium?


A. flexibility
B. holds moisture
C. can be inoculated and poured at a temperature that is not harmful
D. solid at room temperature
E. is digested by most microbes

ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

14. A nutrient medium that has all of its chemical components identified and their precise
concentrations known and reproducible, would be termed
A. complex.
B. reducing.
C. enriched.
D. chemically-defined.
E. None of the choices is correct.

ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

2-6
Chapter 02 - Tools of the Laboratory: The Methods for Studying Microorganisms

15. A nutrient medium that contains at least one ingredient that is NOT chemically definable
would be termed
A. complex.
B. reducing.
C. enriched.
D. synthetic.
E. None of the choices is correct.

ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

16. All of the following are examples of different types of microbiological media except
A. broth.
B. enriched.
C. agar.
D. petri dish.
E. selective.

ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.02 Name and define the three ways to categorize media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

2-7
Chapter 02 - Tools of the Laboratory: The Methods for Studying Microorganisms

17. A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a


culture medium. Following incubation, only the E. coli grows in the culture. What is the most
likely explanation?
A. The microbiologist used too much inoculum.
B. The culture is contaminated.
C. The incubation temperature was incorrect.
D. The culture medium must be selective.
E. The culture medium must be differential.

ASM Objective: 07.01b Ability to apply the process of science: Analyze and interpret results from a variety of microbiological methods, and
apply these methods to analogous situations.
ASM Topic: Module 07 Scientific Thinking
Bloom's Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

18. A common medium used for growing fastidious bacteria is


A. blood agar.
B. trypticase soy agar.
C. mannitol salt agar.
D. MacConkey medium.
E. a reducing medium.

ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

2-8
Chapter 02 - Tools of the Laboratory: The Methods for Studying Microorganisms

19. A reducing medium contains


A. sugars that can be fermented.
B. extra oxygen.
C. hemoglobin, vitamins, or other growth factors.
D. substances that remove oxygen.
E. inhibiting agents.

ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based, molecular and serological).
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

20. Which type of medium is able to distinguish different species or types of microorganisms
based on an observable change in the colonies or in the medium?
A. differential
B. selective
C. enumeration
D. enriched
E. reducing

ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based, molecular and serological).
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

21. A microbiologist decides to use a nutrient medium that contains thioglycollic acid. What
type of microbe is she attempting to culture?
A. fastidious
B. gram-positive
C. anaerobic
D. gram-negative
E. aerobic

ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based, molecular and serological).
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

2-9
Chapter 02 - Tools of the Laboratory: The Methods for Studying Microorganisms

22. Differential media results in which of the following growth characteristics?


A. different color colonies
B. different media color post incubation
C. precipitates
D. gas bubbles
E. All of the choices are correct.

ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based, molecular and serological).
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

23. A reducing media is used to culture


A. fastidious organisms.
B. aerobic organisms.
C. anaerobic organisms.
D. any pathogenic organisms.
E. None of the choices is correct.

ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based, molecular and serological).
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

24. For which bacterial genus does mannitol salt agar differentiate between species?
A. Salmonella
B. Streptococcus
C. Neisseria
D. Staphylococcus
E. Escherichia

ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based, molecular and serological).
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

2-10
Chapter 02 - Tools of the Laboratory: The Methods for Studying Microorganisms

25. A microbiologist must culture a patient's feces for intestinal pathogens. Which of the
following would likely be present in selective media for analyzing this fecal specimen?
A. NaCl
B. sheep red blood cells
C. bile salts
D. thioglycollic acid
E. peptone

ASM Objective: 08.03 Use appropriate methods to identify microorganisms (media-based, molecular and serological).
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

26. Bacteria that require special growth factors and complex nutrients are termed
A. aerobic.
B. anaerobic.
C. fastidious.
D. microaerophilic.
E. autotrophic.

ASM Objective: 03.03 The survival and growth of any microorganism in a given environment depends on its metabolic characteristics.
ASM Topic: Module 03 Metabolic Pathways
Bloom's Level: 01. Remember
Learning Objective: 02.03 Provide examples for each of the three categories of media.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

2-11
Chapter 02 - Tools of the Laboratory: The Methods for Studying Microorganisms

27. A microbiologist inoculates Staphylococcus aureus into a culture medium. Following


incubation, both Staphylococcus aureus and Staphylococcus epidermidis are determined to be
growing in this culture. What is the most likely explanation?
A. The microbiologist used too much inoculum.
B. The culture is contaminated.
C. The incubation temperature was incorrect.
D. The culture medium must be selective.
E. The culture medium must be differential.

ASM Objective: 07.01b Ability to apply the process of science: Analyze and interpret results from a variety of microbiological methods, and
apply these methods to analogous situations.
ASM Topic: Module 07 Scientific Thinking
Bloom's Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I's mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

28. Newly inoculated cultures must be at a specific temperature to encourage growth.


A. streaked
B. poured
C. incubated
D. contaminated
E. All of the choices are correct.

ASM Objective: 08.02 Use aseptic and pure culture techniques to enrich for and isolate microorganisms.
ASM Topic: Module 08 Microbiology Skills
Bloom's Level: 01. Remember
Learning Objective: 02.01 Explain what the Five I's mean and what each step entails.
Section: 02.01
Topic: Tools and Methods of Culturing, Classifying, and Identifying Microorganisms

2-12
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