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Sensory Shelf Life
Estimation of
Food Products
© 2010 by Taylor and Francis Group, LLC
Sensory Shelf Life
Estimation of
Food Products
Guillermo Hough
Boca Raton London New York
CRC Press is an imprint of the
Taylor & Francis Group, an informa business
© 2010 by Taylor and Francis Group, LLC
CRC Press
Taylor & Francis Group
6000 Broken Sound Parkway NW, Suite 300
Boca Raton, FL 33487-2742
© 2010 by Taylor and Francis Group, LLC
CRC Press is an imprint of Taylor & Francis Group, an Informa business
No claim to original U.S. Government works
Printed in the United States of America on acid-free paper
10 9 8 7 6 5 4 3 2 1
International Standard Book Number: 978-1-4200-9291-2 (Hardback)
This book contains information obtained from authentic and highly regarded sources. Reasonable efforts
have been made to publish reliable data and information, but the author and publisher cannot assume
responsibility for the validity of all materials or the consequences of their use. The authors and publishers
have attempted to trace the copyright holders of all material reproduced in this publication and apologize to
copyright holders if permission to publish in this form has not been obtained. If any copyright material has
not been acknowledged please write and let us know so we may rectify in any future reprint.
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Trademark Notice: Product or corporate names may be trademarks or registered trademarks, and are used
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Library of Congress Cataloging-in-Publication Data
Hough, Guillermo.
Sensory shelf life estimation of food products / author, Guillermo Hough.
p. cm.
“A CRC title.”
Includes bibliographical references and index.
ISBN 978-1-4200-9291-2 (hardcover : alk. paper)
1. Food--Sensory evaluation. 2. Food--Shelf-life dating. 3. Detectors. 4. Food
spoilage--Risk assessment. 5. Food--Testing. 6. Food industry and trade--Quality
control. I. Title.
TX546.H68 2010
664’.072--dc22 2010011677
Visit the Taylor & Francis Web site at
http://www.taylorandfrancis.com
and the CRC Press Web site at
http://www.crcpress.com
© 2010 by Taylor and Francis Group, LLC
DEDICATION
To my parents Mary and Ken,
To my wife Adriana,
To my children Julieta, Natalia and Tomás.
© 2010 by Taylor and Francis Group, LLC
Contents
Preface............................................................................................................... xiii
Acknowledgments............................................................................................xv
Author.............................................................................................................. xvii
Chapter 1 Introduction.................................................................................. 1
1.1 Sensory shelf life definition.................................................................... 1
1.2 Labeling regulations................................................................................ 2
1.3 Shelf life of foods is sensory shelf life................................................... 3
1.4 Importance of the consumer in defining food quality........................ 4
1.5 Books on shelf life of foods..................................................................... 6
1.5.1 Labuza (1982)............................................................................... 6
1.5.2 Gacula (1984)................................................................................ 9
1.5.3 IFST guidelines (1993).............................................................. 10
1.5.4 Man and Jones (1994)................................................................ 11
1.5.5 Taub and Singh (1998).............................................................. 14
1.5.6 Kilcast and Subramaniam (2000a).......................................... 16
1.5.7 Eskin and Robinson (2001)...................................................... 18
1.5.8 Labuza and Szybist (2001)....................................................... 18
References.......................................................................................................... 20
Chapter 2 Principles of sensory evaluation............................................. 23
2.1 Introduction............................................................................................. 23
2.2 Definition of sensory evaluation.......................................................... 23
2.2.1 Analyze and interpret.............................................................. 23
2.2.2 Other materials......................................................................... 24
2.2.3 Sight, touch, and hearing......................................................... 24
2.3 Sensory analysis: Trained panels versus experts............................... 25
2.4 General requirements and conditions for sensory tests................... 28
2.4.1 Testing area................................................................................ 28
2.4.2 Lighting...................................................................................... 30
2.4.3 Time of day................................................................................ 31
2.4.4 Carriers....................................................................................... 32
2.4.5 Temperatures of samples......................................................... 32
vii
© 2010 by Taylor and Francis Group, LLC
viii Contents
2.4.6 Sample size................................................................................ 32
2.4.7 Number of samples.................................................................. 33
2.4.8 Coding and order of presentation.......................................... 33
2.4.9 Palate cleansers......................................................................... 33
2.5 Physiological factors............................................................................... 34
2.6 Psychological factors.............................................................................. 34
2.6.1 Expectation error...................................................................... 34
2.6.2 Error of habituation.................................................................. 35
2.6.3 Stimulus error........................................................................... 36
2.6.4 Logical error.............................................................................. 36
2.6.5 Halo effect.................................................................................. 36
2.6.6 Positional bias............................................................................ 36
2.6.7 Contrast effect and convergence error................................... 37
2.6.8 Mutual suggestion.................................................................... 37
2.6.9 Lack of motivation.................................................................... 37
2.6.10 Capriciousness versus timidity.............................................. 38
2.7 Sensory evaluation methods................................................................. 39
2.7.1 Discrimination tests................................................................. 39
2.7.1.1 Triangle test.............................................................. 39
2.7.1.2 Example of sensory shelf life (SSL)
determined by a triangle test................................. 40
2.7.1.3 Paired comparison test............................................ 44
2.7.1.4 Difference from control test................................... 44
2.7.2 Descriptive tests........................................................................ 47
2.7.3 Affective tests............................................................................ 54
2.7.3.1 Selecting consumers................................................ 55
2.7.3.2 Test location.............................................................. 58
2.7.3.3 Quantitative affective test methods...................... 59
References.......................................................................................................... 60
Chapter 3 Design of sensory shelf-life experiments............................. 63
3.1 Initial considerations.............................................................................. 63
3.2 Approximations of shelf-life values..................................................... 64
3.2.1 Literature values....................................................................... 64
3.2.2 Values from the Internet.......................................................... 65
3.2.3 Values based on distribution times........................................ 66
3.3 Temperatures and storage times.......................................................... 67
3.3.1 Temperatures............................................................................. 67
3.3.2 Maximum storage time............................................................ 68
3.3.3 Time intervals........................................................................... 69
3.3.4 Critical descriptor..................................................................... 70
3.3.5 Storing fresh samples............................................................... 71
© 2010 by Taylor and Francis Group, LLC
Contents ix
3.3.6 Basic and reversed storage designs........................................ 73
3.3.6.1 Basic design.............................................................. 73
3.3.6.2 Reversed storage design......................................... 75
3.3.7 How much sample should be stored for sensory shelf
life studies?................................................................................ 79
3.3.7.1 Bananas..................................................................... 79
3.3.7.2 Vegetable oil.............................................................. 80
3.3.7.3 Yogurt........................................................................ 81
References.......................................................................................................... 82
Chapter 4 Survival analysis applied to sensory shelf life................... 83
4.1 What is survival analysis?..................................................................... 83
4.2 Censoring................................................................................................. 84
4.2.1 Right-censoring......................................................................... 84
4.2.2 Left-censoring........................................................................... 84
4.2.3 Interval-censoring.................................................................... 85
4.3 Survival and failure functions.............................................................. 86
4.4 Shelf life centered on the product or on its interaction with
the consumer?......................................................................................... 88
4.5 Experimental data used to illustrate the methodology.................... 89
4.6 Censoring in shelf-life data................................................................... 90
4.7 Model to estimate the rejection function............................................ 93
4.8 Calculations using the R statistical package...................................... 96
4.9 Interpretation of shelf-life calculations............................................. 103
4.10 An additional example......................................................................... 105
4.11 Should consumers be informed?.........................................................110
4.12 Is there a way to deal with totally new products?............................110
References.........................................................................................................111
Chapter 5 Survival analysis continued: Number of consumers,
current status data, and covariates....................................... 113
5.1 Number of consumers..........................................................................113
5.2 Current status data................................................................................114
5.2.1 Introduction..............................................................................114
5.2.2 Experimental data...................................................................116
5.2.3 Model and data analysis.........................................................116
5.2.4 Conclusions on current status data...................................... 122
5.3 Introducing covariates in the model.................................................. 124
5.3.1 Consumer demographics...................................................... 124
5.3.1.1 Experimental data.................................................. 124
5.3.1.2 Covariate model..................................................... 124
5.3.1.3 Calculations using R............................................. 126
© 2010 by Taylor and Francis Group, LLC
x Contents
5.3.2 Product formulations............................................................. 132
5.3.2.1 Experimental data.................................................. 133
5.3.2.2 Calculations using R............................................. 133
5.3.3 Quantitative covariates and number of covariates............ 139
5.3.3.1 Experimental data.................................................. 139
5.3.3.2 Calculations using R............................................. 140
5.3.4 Number of covariates............................................................. 145
References........................................................................................................ 145
Chapter 6 Cut-off point (COP) methodology........................................ 147
6.1 When is the survival statistics methodology difficult to apply?.... 147
6.2 Basics of the COP methodology......................................................... 148
6.3 Approaches in establishing a COP..................................................... 149
6.4 Methodology to measure the COP..................................................... 151
6.4.1 Critical descriptors................................................................. 151
6.4.2 Preparation of samples with increasing levels of
sensory defects........................................................................ 152
6.4.3 Determination of intensity levels of samples by a
trained sensory panel............................................................ 152
6.4.4 Determination of acceptability levels of the same
samples by a consumer panel............................................... 153
6.4.5 Calculation of the COP.......................................................... 154
6.5 Introduction to kinetics....................................................................... 159
6.5.1 Zero-order kinetics................................................................. 159
6.5.2 First-order kinetics................................................................. 159
6.5.3 Choosing between zero- and first-order kinetics.............. 160
6.5.4 Sensory properties that present a lag phase........................161
6.6 Using the COP to estimate shelf life...................................................162
6.6.1 Sample storage and trained sensory panel evaluations.....162
6.6.2 Results and calculations........................................................ 163
6.7 Instrumental COPs............................................................................... 166
6.8 Caveats for using COP methodology................................................ 166
References.........................................................................................................167
Chapter 7 Accelerated storage.................................................................. 169
7.1 Introduction........................................................................................... 169
7.1.1 Acceleration factor fallacy..................................................... 169
7.1.2 Methods of acceleration......................................................... 170
7.2 Arrhenius equation and activation energy....................................... 171
7.2.1 Arrhenius equation................................................................ 171
7.2.2 Data for activation energy calculations............................... 172
7.2.3 Simple activation energy calculations................................. 173
7.2.4 Activation energy calculations based on non-linear
regression..................................................................................176
© 2010 by Taylor and Francis Group, LLC
Contents xi
7.3 The use of Q10........................................................................................ 182
7.4 Survival analysis accelerated storage model.................................... 184
7.4.1 Accelerated storage model..................................................... 184
7.4.2 Experimental data.................................................................. 186
7.4.3 Calculations using R.............................................................. 187
7.5 Potential pitfalls of accelerated shelf-life testing.............................. 193
7.5.1 Pitfall 1: Multiple deterioration modes................................ 194
7.5.2 Pitfall 2: Failure in quantifying uncertainty....................... 194
7.5.3 Pitfall 3: Degradation and rejection affected by
unforeseen variables.............................................................. 195
7.5.4 Pitfall 4: Masked rejection mode.......................................... 195
7.5.5 Pitfall 5: Comparisons that do not hold............................... 196
7.5.6 Pitfall 6: Increasing temperature can cause
deceleration.............................................................................. 197
7.5.7 Pitfall 7: Drawing conclusions from pilot-plant
samples..................................................................................... 198
7.6 Conclusion on accelerated testing...................................................... 199
References........................................................................................................ 199
Chapter 8 Other applications of survival analysis in food
quality........................................................................................ 201
8.1 Consumer tolerance limits to a sensory defect................................ 201
8.1.1 Survival analysis model........................................................ 201
8.1.2 Experimental data used to illustrate the methodology.... 203
8.1.3 Rejection probability calculations........................................ 204
8.1.4 Conclusions............................................................................. 208
8.2 Optimum concentration of ingredients in food products.............. 208
8.2.1 Survival analysis model........................................................ 209
8.2.2 Experimental data used to illustrate the methodology.... 212
8.2.3 Optimum color calculations.................................................. 212
8.2.4 Conclusions on optimum color estimations....................... 221
8.3 Optimum salt level in French bread.................................................. 222
8.3.1 Experimental data used to illustrate the methodology.... 222
8.3.2 Survival analysis model........................................................ 224
8.3.3 Optimum salt concentration calculations........................... 225
8.3.4 Conclusions on optimum salt concentration
estimation................................................................................ 228
8.4 Internal cooking temperature of beef................................................ 229
8.5 Optimum ripening times of fruits..................................................... 232
References........................................................................................................ 236
Index................................................................................................................. 239
© 2010 by Taylor and Francis Group, LLC
Preface
In the year 2000 we started a four-year project on sensory shelf life (SSL)
financed by Ciencia y Tecnología para el Desarrollo (CYTED), Madrid,
Spain. The project included participants from Argentina, Chile, Colombia,
Costa Rica, Spain, and Uruguay. Thanks to this project we were able to
develop most of the methodology that is presented in this book. One of
the most valuable collaborations came from a nonfood-science group
from Cataluña in Spain directed by Dr. Guadalupe Gómez. This group
specializes in survival analysis statistics and at the time worked with
data from AIDS infection and had no connection with SSL methodology.
The exchange with this group allowed the application of survival analy-
sis methodology applied to SSL, which comprises more than three chap-
ters of this book. As a result of the CYTED project, a book was published
(Hough, G. and S. Fiszman, ed. 2005. Estimación de la Vida Util Sensorial de
los Alimentos. Madrid: programa CYTED) which was a worthy predeces-
sor of the present book.
This book starts with an introduction (Chapters 1 and 2) which includes
definitions and a review of books published on the theme of food shelf life
and the basics of sensory analysis and how they apply to shelf life studies.
Experimental design aspects are covered in Chapter 3. Survival analysis
methodology is covered extensively with the basic model (Chapter 4) and
its extensions (Chapter 5). Detailed instructions and software functions are
presented which will allow readers to perform their own SSL estimations.
Data sets used in examples and the R statistical package functions can be
downloaded from the editor’s website (http://www.crcpress.com/). The
cut-off point methodology used to estimate SSL is presented in Chapter
6. Many researchers have a special interest in accelerated shelf-life test-
ing and the methodology is covered in Chapter 7 including R function
to perform a non-linear regression to better estimate activation energy.
Potential pitfalls of accelerated studies are discussed. Finally, Chapter 8
presents extensions of survival analysis �statistics to other areas of food
quality. Optimum concentrations of ingredients and optimum cooking
temperatures are among examples presented.
xiii
© 2010 by Taylor and Francis Group, LLC
Acknowledgments
Thanks to Susana Fiszman for sharing the editing of a book that served
as foundation to this one; Guadalupe Gómez, Malu Calle, Klaus Langohr,
and Carles Serrat for believing survival analysis could be applied to SSL;
and Lorena Garitta, Mercedes López-Osornio, and Miriam Sosa, present
students and collaborators who contributed with experimental work, cal-
culations, and model developments.
xv
© 2010 by Taylor and Francis Group, LLC
Author
Guillermo Hough, Ph.D., is a research scientist with the Comisión de
Investigaciones Científicas and works in the Instituto Superior ExperiÂ�
mental de Tecnología Alimentaria in the small town of Nueve de Julio,
Provincia de Buenos Aires, Argentina.
Dr. Hough conducts his research in the area of sensory science and
has become an expert in the field of sensory shelf life, having published 20
refereed articles on this theme. His courses on sensory science and shelf
life have been very popular in Argentina, Chile, Colombia, Italy, México,
New York, Paris, and Peru. His group is in close contact with local and
regional food companies, performing a wide range of sensory tests and
panel training.
xvii
© 2010 by Taylor and Francis Group, LLC
chapter 1
Introduction
1.1â•…Sensory shelf life definition
Different sources provide different definitions of shelf life. For example
Wikipedia (http://en.wikipedia.org/wiki/Shelf_life, accessed Septem�
ber 5, 2008) says that “shelf life is the time that products can be stored,
during which the defined quality of a specified proportion of the goods
remains acceptable under expected (or specified) conditions of distribu-
tion, storage and display. Shelf life is different from expiration date; the
former relates to food quality, the latter to food safety. A product that has
passed its shelf life might still be safe, but quality is no longer guaranteed.”
Another definition was given by Fu and Labuza (1993): “The shelf life of
a food is the time period for the product to become unacceptable from
sensory, nutritional or safety perspectives.” Ellis (1994) defined shelf life
of a food product as “the time between the production and packaging of
the product and the point at which it becomes unacceptable under defined
environmental conditions.” The IFST Guidelines (1993) defined shelf life
as the time during which the food product will (a) remain safe; (b) retain
desired sensory, chemical, physical and microbiological characteristics;
and (c) comply with any label declaration of nutritional data, when stored
under the recommended conditions. This last definition identifies the
key factors that must be considered when assessing shelf life. The previ-
ous definitions are of shelf life as a whole, that is, covering to a lesser or
larger degree microbiological, physicochemical, nutritional, and sensory
aspects. The ASTM E2454 Standard (2005) defines sensory shelf life (SSL)
as: “… the time period during which the product’s sensory characteris-
tics and performance are as intended by the manufacturer. The product is
consumable or usable during this period, providing the end-user with the
intended sensory characteristics, performance, and benefits.”
The shelf-life limits of some of the factors mentioned in the defini-
tions are defined at a laboratory level without the intervention of the con-
sumer. For example, a central issue in declaring a food to be safe is that it
must be free of pathogenic bacteria and this can be assessed by standard
microbiological analysis. Another example is vitamin D–enriched milk,
which must comply with a certain concentration measured in an analyti-
cal laboratory. In the shelf-life definitions the limit to sensory properties is
© 2010 by Taylor and Francis Group, LLC
2 Sensory shelf life estimation of food products
referred to as acceptable/unacceptable or desired or intended. These lim-
its can be highly ambiguous if not thoroughly researched. Manufacturers
who have to comply with food regulations and, more importantly, comply
with quality standards, need practical and reliable methods to estimate
the shelf lives of their products.
1.2â•…Labeling regulations
Regulations on labeling of shelf-life information on food products vary
from country to country. In Argentina (Código Alimentario Argentino
2005) it is compulsory, though what the label actually says is quite flexible.
Manufacturers can choose from use by, best before, or expires. Some prod-
ucts are exempted from shelf-life labeling: wine, beverages with an alco-
holic content above 10% (v/v), bread that is usually sold and consumed
within 24 hours, vinegar, sugar, sweets, and salt.
In the United States (USDA 2008), except for infant formula and some
baby food, product dating is not generally required by federal regulations.
If a calendar date is shown, immediately adjacent to the date must be a
phrase explaining the meaning of that date such as sell by or use before.
There is no uniform or universally accepted system used for food dating
in the United States. Open dating (use of a calendar date as opposed to a
code) is found primarily on perishable foods such as meat, poultry, eggs,
and dairy products. Coded dating might appear on shelf-stable products
such as cans and boxes of food. Because the expiration date is not indicative
of product quality if storage conditions have been less than optimal, the
Food and Drug Administration (FDA) does not require expiration dates on
most products. An exception to this is that expiration dates are required on
drugs. The dates required on infant formula products are use by dates, not
expiration dates. A consumer using the infant formula product before this
date is assured that the product meets nutritional and quality standards
(http://www.cfsan.fda.gov/~dms/qa-ind7c.html, accessed April 13, 2009).
In the United Kingdom (Food Standards Agency 2008) it is compulsory
for food products to have “an appropriate durability indication.” There is
also a clear distinction between use by and best before dates. Use by means
that any food or drink should not be consumed after the date shown on
the label. Even if it looks and smells fine, using it after this date could put
health at risk. Use by dates are usually placed on foods that go bad quickly,
such as milk, soft cheese, ready-prepared salads, and smoked fish. Best
before dates are usually used on foods that last longer, such as frozen,
dried, or canned foods. It should be safe to eat food after the best before
date, but the food will no longer be at its best. After this date, the food
might begin to lose its sensory quality.
No doubt other countries, or states within countries, have varying reg-
ulations on shelf-life labeling. Gone are the days when food was bought at
© 2010 by Taylor and Francis Group, LLC
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