Iarjset 2019 61012
Iarjset 2019 61012
Abstract: Jackfruits are very versatile in their uses for food and as a folk medicine. Most parts of the jackfruit
including pulp and seeds are edible. Three cultivars jackfruit (Artocarpus heterophyllus Lam.) namely: SS1, SS2 and
SS3 which cultivated in Aswan, Egypt, were studied for their physical properties, minerals, chemical composition,
polyphenolic compounds, ascorbic acid, alpha tocopherol and beta carotene contents. Also, jams were prepared from
mango pulp and jack fruit pulp, as well as, sensory evaluation, chemical composition and storage for six months for
these jams was determined. The results revealed that TSS (%), TTA (% DW) contents ranged from 24.21-26.23 and
0.37-0.46; respectively. The non-reducing sugars, reducing sugars were found in studied pulps with higher contents
ranging 30.64-33.75%and20.57-22.14%; respectively, while the total sugars ranged between 52.78 to 54.32%. The
concentration of potassium was significantly (P<0.05) higher (1928.84) in SS3 followed by SS2 (1899.82) and SS1
(1897.66). The vitamin C content (mg/100g DW) in three strains of jackfruit pulp was ranged from 30.44 to 30.66.
Jackfruit pulps contained reasonable amounts of anioxidative components especially vitamin A and polyphenolics. The
jam made from jackfruit pulp and mango pulp with ratio 1:1 give a better acceptable product with good properties, it
had the highest in total, reducing sugars with values 89.63 and 26.89 g/100g DW, respectively. The T.S.S and T.T.A
were slightly increased during 6 months with no significance differences between jackfruit and mango jams. There is a
decrease in total and non-reducing contents during storage but reducing sugars was increased in jam samples. The
results showed that consumption of jackfruit can enhance the amounts of phytochemical compounds such as vitamins A
and C consumed. In addition, the importance of this study is to prepare jackfruit jam that will be characteristically
accepted by consumer.
I. INTRODUCTION
The jackfruit (Artocarpus heterophyllus Lam.) is traditionally a tropical fruit used as a nation medicine for the
treatment of tapeworm infection, malarial fever, diabetes, diarrhea and other ailments as well as for food. The
Artocarpus species were found mostly in South-East Asia, particularly in Philippines, India, Thailand, Indonesia and
Malaysia.
Based on their plant structure, 8-15% of the fruit mass is represented by seeds surrounded by brown spermoderm which
it covers the white cotyledon that is high in starch and protein. The fruit of most jackfruit cultivars weights 10–30 kg,
although the full range of known cultivars is 2–36 kg and even heavier. Based on fruit quality, the fruit is mostly
divided into two main varieties the first one have strong smell soft very sweet pulp with thin fibrous texture and the
second is thick, firm, often crisp, less fragrant pulp (Prakash et al., 2009; Dasaesamoh and Seechamnanturakit 2014).
The jackfruit was consists of about 54% crust 29% pulp and 12% seed. The inner part of the ripe fruit has a large sweet,
tasty yellow bulb, clustered between narrow strips and a central pity heart consisting of 25-30% of the total fruit. Edible
bulbs of mature grapefruit are consumed for their wonderful taste and enjoyable smell, which it contain high levels of
thiamine, calcium starch and protein. It is also rich in energy, dietary fiber, minerals and vitamin (Eke-Ejiofor et al.,
2014).
Fresh fruits are a good source of iron, magnesium, potassium and manganese, as well as contain small amounts of
flavonoids and vitamin A which these compounds play vital, an important role in vision and as antioxidants. The
composition (wet weight) of Jack fruit pulp contained average total solids 23.69%, acidity 0.25%, total sugar 17.78%,
protein 1.75%, ash 0.93% and fat 0.22%. Depending on high nutritive value of jackfruit pulp it eaten as fresh and used
in fruit salads. The mature pulp of jackfruit (100 g) contains moisture 77%, carbohydrate 18.9 g, protein 1.9 g, fiber 1.1
g, fat 0.1 g, ash 0.8 g, potassium 191 to 407mg, phosphorus 38 to 41mg, calcium 20 to 37mg, iron 0.50 to 1.70 mg,
vitamin A 175 to 540 I.U and vitamin C 12 to 14 mg with a caloric value of 84 calories (Mondal et al., 2013; Jagdeesh
et al., 2007; Naik et al., 2017).
Fruits contain vitamin A precursor beta-carotene which converted to vitamin A easily. Vitamin A deficiency reduces
the body's resistance to infection. In developing countries, carotenoids (pro-vitamin) in fruits and vegetables are the
main source of vitamin A for 70-90% of people. Among carotenoids, lycopene, lutein and beta-carotene are the highest
in antioxidant properties (Umesh et al., 2010, Swami et al., 2012).
Materials: The studied fruits used in this investigation were selected seed strains of breeding program to improve the
pomological characteristics of these jack-fruit fruits, namely SS1, SS2 and SS3 for the golden black cultivar, which
purchased from the Tropical Farm at Agricultural Research Station - Kom Ombo - Aswan Governorate - Upper Egypt
during season 2018.
Jack fruit pulp preparation; The fruit pulp was collected from the seeds and dried under vacuum at 50 °C for 24
hours. The dried pulp was grinded in a laboratory mill to obtain complete flour from it, then stored at 4 ° C until
analyzed.
Methods
Proximate composition analysis
Physical properties
pH value, T.S.S (total soluble solids) and T.T.A (total titratable acidity) contents were determined as described by
A.O.A.C (2000) methods.
Chemical composition: Moisture, ash, crude oil, crude protein, total sugars, non-reducing sugars and reducing sugars
were determined as described in the AOAC Methods (2000).
Minerals content: Contents of Ca, Mg, Fe, Zn, Cu, Mn, Se, Ni, Cr, Pb, As and Cd in the studied samples were
determined by iCAP6200 (ICP-OES) Inductively Coupled Plasma Emission Spectrometry (Isaac and Johnson. 1985). A
flame photometer corning 400 was used to determine Na and K; while, spectrophotometer was used for determination
of P content (Jackson, 1967) after wet ashing as recording in AOAC (2000).
Polyphenolic compounds: The total, bound and free phenolic compounds contents of samples were determined using
modified Folin-Ciocâlteu colorimetric method (Singleton et al., 1999).
Ascorbic acid content: The method described by Sahlin et al., (2004) was used for ascorbic acid determination. The
results were calculated as mg/100 g dry weight (DW).
(Whatman, Maidstone, England) filter and analysed using a reversed phase HPLC using μ Banda Pak C18 (3.9 x 300
mm) column and acetonitrile-methanol-ethyl acetate (88:10:2) as the mobile phase. The eluted β-carotene eluted was
detected and quantified using a UV-Visible detector attached to the 600 controller model (Waters, Milford, MA, USA)
HPLC. The peak areas of appropriate standard (β-carotene) and retention time (rt) was used to identify and quantify
the isolated β-carotene.
*A = Jack fruit jam made with 50% mango pulp. **B = Jack fruit jam made with 100% jack fruit pulp.
The fruits were washed, peeled and cut into small pieces of similar size. Then, the prepared fruit pulp and a little of
sugar was placed in a cooker and blended well. The blend was cooked under constant stirring for 12-15 minutes during
which the remaining sugar and citric acid was added. Then the mixture was cooked until the T.S.S reached 68° Brix.
After that, the heat was turned off and the Jam was cooled to 87°C, filled in sterilized (autoclaved at 121 ºC for 15 min)
glass dry jars.
Storage of jackfruit and mango jam
The studied jam were stored at refrigerator temperature (8 ±1°C) for six months at one month interval to determine
physico-chemical properties: pH, TSS, TTA, TSS/TTA ratio, total sugars, reducing sugars, non-reducing sugars and
vitamin C according to their own methods as previously mentioned.
Sensory evaluation of jam
Jam products were sensory evaluated by a panel of the judges from the staff of Food science and Technology
department, Faculty of Agriculture, Assiut University. International protocols (ISO 6658: 2005) were used to perform
the descriptive analyses. The parameters estimated using ten points hedonic scales were: consistency (10), intensity of
odour (10), homogeneity in colour (10), intensity of taste (10), free of sugar granules (10) & overall acceptability (10).
Statistical analysis:
All data were subjected to statistical analysis using package of SAS. Mean comparisons were done using LSD 5%
(SAS 2000).
Mineral Composition
The mineral composition (mg/100g dry weight) in studied Jackfruit pulp (Table 3) revealed that the concentration of
potassium was significantly (P<0.05) higher (1928.84) in SS3 followed by SS2 (1899.82) and SS1 (1897.66).
Table (3): Mineral composition (mg/100g dry weight) of jack fruit pulp samples.
Analysis Samples LSD 0.05
SS1 SS2 SS3
K 1897.66 1899.82 1928.84 0.22
Ca 134.44 139.42 141.22 0.26
Mg 137.17 125.22 134.00 0.15
P 164.13 172.24 162.45 0.10
Fe 121.23 129.53 130.05 0.06
Na 94.80 88.50 91.60 0.19
Se 1.95 1.98 2.04 0.17
Mn 0.98 0.88 0.92 0.07
Zn 0.87 0.83 0.86 0.05
Cu 0.79 0.78 0.82 0.07
Cr 1.69 1.77 1.73 0.10
Ni 0.72 0.72 0.71 0.05
As 0.15 0.15 0.15 0.08
Pb 0.078 0.082 0.079 0.006
Cd 0.005 0.006 0.005 0.005
There were significant (P<0.05) variations in contents of Ca, Mg, P, Fe which ranged 134.44-141.22, 125.22-137.17,
162.45-172.24 and 121.23-130.05 mg/100g DW; respectively. In addition, the contents of Se, Mn, Zn and Cu were in
the same range with small values. As illustrated in Table (3) the contents of heavy metals in the studied samples was
found in small quantities, which Cr, Ni ranged 1.69-1.77 and 0.71-0.72 mg/100g DW; respectively. The same way was
found for Cd and Pb contents with no significant differences. Similar reports have been made by Manjeshwar et al.,
(2011); Swami et al., (2012) and Tang et al., (2013) in jackfruit pulp. The differences in mineral composition could be
due to environmental conditions and varietal differences.
Table (4): Vitamins (C, A, E) and polyphenolic compounds composition of pulp from three cultivars of jack fruits.
Sample Vit. C (mg/100g Vitamin A Vitamin E Polyphenolic compounds (mg
D.W) (IU)a (IU)b GAE/100g D.W)
Free Bound Total
SS1 30.66 368.08 1.07 34.67 1957.78 1992.45
SS2 30.44 374.28 1.09 64.95 1845.33 1910.28
SS3 30.52 362.82 1.11 51.99 1852.49 1904.48
LSD 0.05 0.16 0.03 0.13 0.07 0.18 0.05
a- One international Unit (IU) of vitamin A = 0.6 mcg. of beta-carotene. b- One international Unit (IU) of vitamin E =
0.67 mg natural alpha-tocopherol.
Table (5) Sensory Evaluation of mango and jack fruit (SS3 based on maturity and taste index) jam.
Sample Consistency Homogeneity Intensity Intensity Free of Over all Total
(10) in colour of of taste sugar acceptability score
(10) odour(10) (10) granules (10) (10) (60)
Control 9.00 9.15 8.80 9.15 9.00 9.00 54.10
A 8.80 8.25 8.50 8.50 9.00 8.30 51.35
B 8.00 7.50 7.50 8.00 9.00 7.50 47.50
LSD 0.05 0.89 0.08 0.19 0.70 0.90 0.18 0.21
Control= Mango jam made with 100% mango pulp. A = Jack fruit jam made with 50% mango pulp. B = Jack fruit jam
made with 100% jack fruit pulp.
jams. The data about jam composition are in accordance with some previous studies in this field (Eke-Ejiofor and
Owuno 2013, Mondal et al., 2013).
Control= Mango jam made with 100% mango pulp. A = Jack fruit jam made with 50% mango pulp. B = Jack fruit jam
made with 100% jack fruit pulp.
T.S.S, T.T.A, T.S.S/T.T.A ratio and pH of stored mango and jackfruit jams
The T.S.S, T.T.A, T.S.S/T.T.A ratio and pH of mango and jackfruit jams stored at refrigerator temperature (8±1°C) are
presented in Figures 2-5. The results revealed that the T.S.S (Fig. 2) and T.T.A (Fig. 3) were slightly increased during 6
months with no significance (P<0.05) differences among mango and jackfruit jams.
Control= mango jam made with 100% mango pulp. A = Jack fruit jam made with 50% mango pulp. B = Jack fruit jam
made with 100% jack fruit pulp.
The T.S.S was ranged from 67.20 to 68.30% for the studied jams while; the T.T.A content of jams was increased from
0.50 to 0.68%. The T.S.S/T.T.A (Fig. 4) ratio was decreased significantly (P<0.05) ranging 125.00-100.44, this
decrease depends on T.S.S and T.T.A values increment.
Consequently, the pH (Fig. 5) value was decreased as a result of T.T.A increment, which ranging 3.30-3.48. During
storage some changes was done for example solubilization of jam contents, pectin hydrolysis, decomposition of
ascorbic acid or increase in ionized weakly acid which caused the increased in T.S.S, T.T.A amounts and decreased in
pH. Our results on these properties are in agreement with some studies in this field (Hussain and Shakir 2010; Khan et
al., 2012; Safdar et al., 2012) which confirmed the importance of acidity, sugars and the sugar/acid ratio in determine
taste; give a technological properties of jams.
Control= mango jam made with 100% mango pulp. A = Jack fruit jam made with 50% mango pulp. B = Jack fruit jam
made with 100% jack fruit pulp.
Total sugars, reducing sugars, non-reducing sugars, vitamin C contents of stored mango and jackfruit jams
Total sugars, reducing sugars, non-reducing sugars, vitamin C contents (on dry weight basis) of mango and jackfruit
jams stored at refrigerator temperature (8±1°C) were illustrated in Figures 6-9.
The data for sugars (Fig. 6, 7, 8) revealed significantly (P<0.05) that there is a decrease in total and non-reducing
contents during storage but reducing sugars was increased in jam samples. As a result of non-reducing inversion which
reproduces glucose, fructose, the reducing sugars content was increased with range24.79-28.74 mg glucose/100g DW.
From Fig. 9 it could be noticed that vitamin C was decreased significantly with range 3.61-5.54 mg/100g DW in the
studied jams during storage. This decrease was a result of oxidation of vitamin C to dehydroascorbic acid. On the other
hand, during processing of Jam by traditional method the vitamin C content was decreased significantly compared with
its content in fresh fruit. The above mentioned data were in accordance with previous studies (Damiani et al., 2012;
Khan et al., 2012; Safdar et al., 2012).
Control= mango jam made with 100% mango pulp. A = Jack fruit jam made with 50% mango pulp. B = Jack fruit jam
made with 100% jack fruit pulp.
Control= mango jam made with 100% mango pulp. A = Jack fruit jam made with 50% mango pulp. B = Jack fruit jam
made with 100% jack fruit pulp.
IV. CONCLUSION
The results of this study concluded that the seeds strains jackfruit cultivars contained high moisture and T.S.S contents.
The physical properties of jackfruit pulp are different which SS3 have highest contents for maturity (65.43) and taste
index (3.64) as compared to the others. The reducing sugars, non-reducing sugars were found in studied pulps with
higher contents ranging 20.57-22.14% and 30.64-33.75%; respectively. While the total sugars ranged between 52.78 to
54.32%. There were significant (P<0.05) differences in contents of K, Ca, Mg, P and Fe, this variation could be due to
varietal differences and environmental conditions. The jackfruit pulps have reasonable amounts of anioxidative
components especially vitamin A and polyphenolics. The jam made from mango pulp and jackfruit pulp with ratio 1:1
gave a better acceptable product with good properties comparing with jam made from jackfruit pulp only. The T.T.A
content of stored jams was increased from 0.50 to 0.68%. Consequently, the pH value was decreased as a result of
T.T.A increment, which ranging 3.30-3.48. There is a decrease in total and non-reducing contents during storage but
reducing sugars was increased in jam samples. So, results confirmed the importance of these strains as a nutritional
material having greater antioxidant activity in retarding oxidation during processing and storage periods.
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